Multi-environment regulation and control in-situ real-time photosynthetic phenotype imaging system

Abstract

The invention relates to a multi-environment regulation and control in-situ real-time photosynthetic phenotype imaging system applied to the field of photosynthetic phenotype imaging of crop plants, which comprises an environment control system, a photoelectric control system, an image acquisition system, a plant light system, a computer, a touch screen and a plant sample cultivated in a detection box, the box type multi-environment regulation and control in-situ real-time photosynthetic phenotype imaging system realizes automatic regulation and control of test environment factors, realizes in-situ real-time monitoring and imaging of chlorophyll fluorescence parameters of plants in a box body in an experiment process, improves the accuracy of chlorophyll fluorescence parameter detection, improves the efficiency of research work, and has a wide application prospect. The detection angle of the fluorescence imaging CCD camera is parallel to the to-be-detected blade by matching with the adjusting belt, the consistency of the detection distance is ensured, the warping part is smoothed by matching with the smoothing unit, and the accuracy of the detection result is ensured.

Description

Technical Field

[0001] The invention relates to an in-situ real-time photosynthetic phenotype imaging system, in particular to a multi-environment controlled in-situ real-time photosynthetic phenotype imaging system applied in the field of crop plant photosynthetic phenotype imaging. Background Art

[0002] Photosynthesis is the basis of crop physiological metabolism and a decisive factor in the formation of yield and quality. The collection and analysis of photosynthetic efficiency information has become an important part of plant phenomics, which will bring important changes to the breeding of stress-resistant and high-yield varieties and the intelligent regulation of greenhouse growth environment. Among them, chlorophyll fluorescence detection of photosynthetic efficiency has the advantages of simple operation, non-destructiveness and rapidity, and has become a conventional means to reflect plant physiological state and photosynthetic efficiency in plant stress physiology research.

[0003] The specification of Chinese invention patent CN202110475367.9 discloses a device and method for detecting chlorophyll fluorescence parameters of plant leaves. Based on the acquired radiance information, fluorescence spectrum and leaf temperature of the plant leaves, the chlorophyll fluorescence parameters are calculated to achieve real-time, non-destructive, efficient and accurate detection of the chlorophyll fluorescence parameters of plant leaves.

[0004] When the existing plant chlorophyll fluorescence detection device is working, the leaves are removed from the plant for detection. This will cause the light environment during detection to be different from the light environment when the plant is growing. In addition, the measurement area of ​​the detection device is limited, and it is impossible to achieve overall imaging of larger plants or groups. The detection of photosynthetic efficiency of plant groups is even more limited. Summary of the invention

[0005] In view of the above-mentioned prior art, the technical problem to be solved by the present invention is to provide a chlorophyll fluorescence imaging system that can precisely control multiple environments and perform high-throughput and large-area chlorophyll fluorescence imaging, so as to realize in-situ detection of plant experiments or simulate the test environment of test plants during the detection process.

[0006] In order to solve the above problems, the present invention provides a multi-environment controlled in-situ real-time photosynthetic phenotype imaging system, comprising an environmental control system, a photoelectric control system, an image acquisition system, a plant light system, a computer, a touch screen and plant samples cultivated in a detection box, wherein the environmental control system is used to control the temperature, humidity, CO2 concentration and light index of the detection box;

[0007] The plant light system includes an LED light board controlled by PWM, and the LED light board is installed on the inner top wall of the detection box to provide plant growth light, measurement light, actinic light and saturation pulse light for plant samples;

[0008] The image acquisition system includes a fluorescent imaging CCD camera, which is used to capture the leaf green fluorescent imaging of the plant samples in the detection box and transmit the captured image data to a computer. The interior of the detection box is formed into a split chamber and a test chamber through a partition. The plant samples are located in the test chamber. A stepper motor is installed inside the split chamber. The output end of the stepper motor is connected to a lead screw that penetrates the test chamber. A moving sleeve is threadedly sleeved on the surface of the lead screw, and a rectangular block is connected to the end of the moving sleeve that is away from the stepper motor. The bottom of the moving sleeve is a control motor, and the output end of the control motor is connected to an electric extension rod, and the power end of the electric extension rod is connected to A frame member, wherein the frame member is internally rotatably connected with a rotating rod, a driving motor is installed on the surface of the frame member, and the output end of the driving motor is connected to one end of the rotating rod, an adjusting belt is wound around the surface of the rotating rod, and the end of the adjusting belt is connected to a fluorescent imaging CCD camera, a smoothing unit is installed at the bottom of the frame member, and the smoothing unit includes a servo motor embedded in the middle of the bottom of the frame member, the output end of the servo motor is connected to a central air pipe through a short shaft, both ends of the central air pipe are connected to elastic air pipes, magnetic sheets are installed on the end surfaces of the two elastic air pipes, and an electromagnetic ring is sleeved on the outer surface of the servo motor;

[0009] The photoelectric control system is used to control the plant light system and the environmental control system, and the touch screen is installed on the front of the detection box.

[0010] In the above-mentioned multi-environment regulation in situ real-time photosynthetic phenotype imaging system, the experimental environmental factors are automatically regulated, and the in situ real-time detection and imaging of plant chlorophyll fluorescence parameters are realized, and intelligent data analysis and image processing are performed simultaneously, thereby improving the accuracy of chlorophyll fluorescence parameter detection and enhancing the efficiency of research work.

[0011] As a further improvement of the present application, the plant growth light is composed of red light in the range of 660±5nm, blue light in the range of 450±5nm and infrared light in the range of 730±5nm, which is used to provide the lighting needs for plant growth. The measured light intensity is 1μmolm -2 s -1 , actinic light intensity is 400~600μmolm -2 s -1 The light energy density of saturation pulse light is 5000~8000μmolm -2 s -1 , and the saturation pulse light is realized through laser and homogenizer during operation.

[0012] As a further improvement of the present application, the actinic light is realized by modulating pulses, and the on time is 0.2 to 1.5 seconds.

[0013] As a further improvement of the present application, a rectangular groove matching the rectangular block is provided inside the partition, the cross-sectional height of the rectangular groove is not less than the sum of the lengths of the movable sleeve, the frame member and the electric extension rod when not started, and the rectangular block passes through the surface of the lead screw.

[0014] As a further improvement of the present application, the interior of the adjustment belt is filled with electrorheological fluid, a distance measuring sensor is installed at the bottom of the fluorescent imaging CCD camera, and the adjustment belt and the driving motor are both connected to the laser distance measuring sensor limit signal.

[0015] As a further improvement of the present application, the surface of the rotating rod is provided with tooth patterns, and the surface of the adjusting belt close to the rotating rod is provided with friction patterns matching the tooth patterns.

[0016] As another improvement of the present application, the working steps of the smoothing unit are as follows:

[0017] S1. First determine the position of the leaf to be tested on the surface of the plant sample, and then move the frame member downward to the top of the leaf to be tested with the cooperation of the electric extension rod;

[0018] S2, using an adjustment belt and a driving motor to adjust the fluorescence imaging CCD camera to be parallel to the leaf to be inspected;

[0019] S3, using a fluorescent imaging CCD camera to capture an image of the surface of the blade to be detected, to detect whether the edge of the blade to be detected is warped, if it is determined that warping occurs;

[0020] S4. If edge warping is determined to have occurred, the image of the blade to be inspected is taken by a fluorescent imaging CCD camera to determine the warping position, the smoothing unit is started, the smoothing direction is adjusted by a servo motor and an electromagnetic ring, and the warped portion is smoothed. Subsequently, the fluorescent imaging CCD camera switches its working state to obtain fluorescent imaging data of the blade to be inspected.

[0021] As another improved supplement of the present application, the elastic air tube is made of elastic and telescopic material, the central air tube is made of rigid material, and air outlets are provided on the surfaces of the elastic air tube and the central air tube.

[0022] As another improved supplement of the present application, the horizontal distance between the bottom of the frame member and the adjustment belt is not less than the horizontal length of the central air pipe, and the top of the magnetic plate is in close contact with the bottom of the frame member.

[0023] In summary, the beneficial effects of this application are as follows:

[0024] 1. To achieve consistency between the experimental treatment environment and the detection environment. When conducting chlorophyll fluorescence imaging detection, precise control of light (light quality, light intensity and illumination time), temperature, humidity and carbon dioxide concentration can be achieved within a certain range to ensure the accuracy of photosynthetic phenotype detection under the conditions of multiple environmental factors of plants;

[0025] 2. Through intelligent control, dark processing and other processes can be automated during chlorophyll fluorescence detection, and the accuracy of chlorophyll fluorescence parameters such as F0 can be improved. At the same time, intelligent control makes it possible to analyze the fluorescence induction curve of variable actinic light (light quality, light intensity), and the relationship between plant growth and development stages and rhythms and chlorophyll fluorescence, which can promote the development of chlorophyll fluorescence analysis technology and the development of different applied disciplines.

[0026] 3. The plant test growth light, measurement light and actinic light share the same LED light board, and the different functions are realized through PWM control, which can ensure the consistency of light quality, light intensity and other light environments during plant test and measurement, and reduce hardware investment costs;

[0027] 4. The test equipment and testing instruments are unified, plant testing and measurement can be carried out simultaneously, achieving in-situ testing without moving, reducing damage to test samples and improving research work efficiency.

[0028] 5. Cooperate with the adjustment belt to make the detection angle of the fluorescent imaging CCD camera parallel to the blade to be detected, ensure the consistency of the detection distance, and cooperate with the smoothing unit to smooth the warped part to ensure the accuracy of the detection result. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a system composition diagram of the first implementation mode of the present application;

[0030] Figure 2 This is a schematic diagram of the external structure of the detection box according to the first embodiment of the present application;

[0031] Figure 3 This is a diagram showing the internal structure of the detection box according to the first embodiment of the present application;

[0032] Figure 4 This is a diagram showing the surface structure installation of the moving sleeve of the first embodiment of the present application;

[0033] Figure 5 This is a schematic diagram of adjusting the angle of a fluorescence imaging CCD camera with an adjustment belt according to the first embodiment of the present application;

[0034] Figure 6 This is a schematic diagram of the tooth pattern and friction pattern of the first embodiment of the present application;

[0035] Figure 7This is a diagram showing a warped leaf edge on the surface of a plant sample according to the second embodiment of the present application;

[0036] Figure 8 This is a schematic diagram of the installation of the central air tube, the elastic air tube and the magnetic plate of the second embodiment of the present application;

[0037] Fig. 9 This is a diagram of the second embodiment of the present application in which the central trachea and the elastic trachea are simultaneously exhausted and smoothed;

[0038] Fig.10 This is a state diagram of the elastic trachea contracting and the central trachea turning for exhaust and smoothing in the second embodiment of the present application.

[0039] Description of the numbers in the figure:

[0040] 1. Detection box; 2. LED light board; 3. Stepper motor; 4. Lead screw; 5. Electric extension rod; 6. Driving motor; 7. Adjustment belt; 8. Fluorescence imaging CCD camera; 9. Control motor; 10. Servo motor; 11. Magnetic plate; 12. Elastic air tube; 13. Centering air tube. DETAILED DESCRIPTION

[0041] Two implementation modes of the present application are described in detail below with reference to the accompanying drawings.

[0042] The first implementation method:

[0043] Figure 1 A multi-environment controlled in-situ real-time photosynthetic phenotype imaging system is shown, comprising an environmental control system, a photoelectric control system, an image acquisition system, a plant light system, a computer, a touch screen, and plant samples cultivated in a detection box 1, wherein the environmental control system is used to control the temperature, humidity, CO2 concentration, and light index of the detection box 1;

[0044] The plant light system includes an LED light board 2 controlled by PWM, and the LED light board 2 is installed on the inner top wall of the detection box 1 to provide plant growth light, measurement light, actinic light and saturated pulse light for plant samples;

[0045] Figure 2-4As shown, the image acquisition system includes a fluorescent imaging CCD camera 8, which is used to capture the leaf green fluorescent imaging of the plant sample in the detection box 1, and transmit the captured image data to the computer. The interior of the detection box 1 is formed into a split chamber and a test chamber through a partition, and the plant sample is located in the test chamber. A stepper motor 3 is installed inside the split chamber, and the output end of the stepper motor 3 is connected to a lead screw 4 that penetrates into the test chamber. A moving sleeve is threadedly sleeved on the surface of the lead screw 4, and a rectangular block is connected to the end of the moving sleeve that is away from the stepper motor 3. The bottom of the moving sleeve controls a motor 9, and the output end of the control motor 9 is connected to an electric extension rod 5. The power end of the electric extension rod 5 is connected to a frame member, and the internal rotation of the frame member is connected to a rotating rod. A drive motor 6 is installed on the surface of the frame member, and the output end of the drive motor 6 is connected to one end of the rotating rod. An adjusting belt 7 is wound around the surface of the rotating rod, and the end of the adjusting belt 7 is connected to the fluorescent imaging CCD camera 8;

[0046] The photoelectric control system is used to control the plant light system and the environmental control system, and the touch screen is installed on the front of the detection box 1.

[0047] Specifically, when performing multi-environment controlled in-situ real-time photosynthetic phenotype imaging operations, since the plant samples are planted inside the detection box 1, in-situ detection can be achieved, shortening the process of sampling - transportation - laboratory - detection, ensuring the effect of real-time detection and the accuracy of detection;

[0048] In addition, the environmental control system is used to adjust the temperature, humidity, light intensity and CO2 concentration in the test box 1 to simulate the environment of the normal growth state of the plant, and the touch screen is used to adjust and control the test environment factor parameters in the test box 1;

[0049] In order to prevent the humidity in the test chamber from interfering with the high-precision stepper motor 3 and related electrical components in the non-testing state, the stepper motor 3 can be started to control the moving sleeve to move on the surface of the lead screw 4 to the split chamber. In this state, the rectangular block can block the rectangular groove that runs through the split chamber and the test chamber, thereby isolating the influence of moisture in the test chamber;

[0050] During detection, the stepper motor 3 is started to drive the fluorescent imaging CCD camera 8 to move through the rectangular slot into the test chamber, and then the electric extension rod 5 is started to move the fluorescent imaging CCD camera 8 downward, and the direction of the fluorescent imaging CCD camera 8 is adjusted by the control motor 9 in coordination with the orientation of the leaves to be detected. After that, the fluorescent imaging CCD camera 8 is horizontally suspended directly above the leaves to be detected. Since the leaves on the surface of the plant are in a non-horizontal placement state when growing (the cucumber plant is taken as an example in this embodiment), after the fluorescent imaging CCD camera 8 is horizontally suspended, the drive motor 6 can be started to control the rotation of the rotating rod, thereby driving the adjustment belt 7 to adjust the length of the two ends, thereby achieving an angle adjustment effect on the fluorescent imaging CCD camera 8 (such as Figure 5 As shown), the inclination angle of the fluorescent imaging CCD camera 8 is kept parallel to the blade to be detected, thereby ensuring the consistency of the detection distance and the reliability of the detection result;

[0051] In addition, the blade area is relatively large, and the moving sleeve and the fluorescent imaging CCD camera 8 need to be driven to move with the cooperation of the stepping motor 3, so as to realize multi-site detection and separate imaging and then splicing, so as to obtain a relatively complete detection image.

[0052] Plant growth light is composed of red light in the range of 660±5nm, blue light in the range of 450±5nm and infrared light in the range of 730±5nm, which is used to meet the lighting needs of plant growth. The measured light intensity is 1μmolm -2 s -1 , actinic light intensity is 400~600μmolm -2 s -1 The light energy density of saturation pulse light is 5000~8000μmolm -2 s -1 , and the saturation pulse light is realized through laser and homogenizer during operation.

[0053] Actinic light is achieved by modulating pulses, with a switching time of 0.2 to 1.5 seconds.

[0054] A rectangular groove matching the rectangular block is provided inside the partition, the cross-sectional height of the rectangular groove is not less than the sum of the lengths of the movable sleeve, the frame member and the electric extension rod 5 when not started, and the rectangular block passes through the surface of the lead screw 4.

[0055] Specifically, the rectangular groove is used to pass through the test chamber and the split chamber, and when the electric extension rod 5 is not started, the rectangular groove corresponding to the rectangular block can drive the moving sleeve and related components connected on the surface to pass through the rectangular groove smoothly to realize object transfer.

[0056] The interior of the adjustment belt 7 is filled with electrorheological fluid, a distance measuring sensor is installed at the bottom of the fluorescent imaging CCD camera 8, and the adjustment belt 7 and the driving motor 6 are both connected to the laser distance measuring sensor limit signal.

[0057] Specifically, when the adjustment belt 7 is used to adjust the angle of the fluorescent imaging CCD camera 8, the distance values ​​of multiple position points on the surface of the fluorescent imaging CCD camera 8 (that is, the position points where the ranging sensor is located) and the surface of the blade to be measured are used. When the distance values ​​of multiple position points are consistent, the electrorheological fluid inside the adjustment belt 7 is energized, which can solidify the adjustment belt 7 and have a solidifying and restraining effect on the fluorescent imaging CCD camera 8, thereby avoiding subsequent shaking that may cause changes in the detection area of ​​the fluorescent imaging CCD camera 8, thereby ensuring detection accuracy.

[0058] Figure 6 As shown, the surface of the rotating rod is provided with tooth patterns, and the surface of the adjusting belt 7 close to the rotating rod is provided with friction patterns matching the tooth patterns.

[0059] Specifically, the design of the tooth pattern and the friction pattern can enable the adjusting belt 7 to move stably and synchronously when the rotating rod rotates, thereby achieving an effect of adjusting the end length.

[0060] The second implementation method:

[0061] Figure 8 It is shown that a smoothing unit is installed at the bottom of the frame member, and the smoothing unit includes a servo motor 10 embedded in the middle position of the bottom of the frame member. The output end of the servo motor 10 is connected to a central air pipe 13 through a short shaft. Both ends of the central air pipe 13 are connected to elastic air pipes 12. Magnetic plates 11 are installed on the end surfaces of the two elastic air pipes 12. The outer surface of the servo motor 10 is sleeved with an electromagnetic ring.

[0062] The elastic air tube 12 is made of elastic and telescopic material, the central air tube 13 is made of rigid material, and air outlets are arranged on the surfaces of the elastic air tube 12 and the central air tube 13 .

[0063] The horizontal distance between the bottom of the frame and the adjustment belt 7 is not less than the horizontal length of the central air pipe 13, and the top of the magnetic plate 11 is in close contact with the bottom of the frame.

[0064] The working steps of the smoothing unit are as follows:

[0065] S1, first determine the position of the leaf to be tested on the surface of the plant sample, and then move the frame member downward to the top of the leaf to be tested with the cooperation of the electric extension rod 5;

[0066] S2, using the adjustment belt 7 and the drive motor 6 to adjust the fluorescent imaging CCD camera 8 to be parallel to the leaf to be detected;

[0067] S3, using the fluorescent imaging CCD camera 8 to capture an image of the surface of the blade to be detected, to detect whether the edge of the blade to be detected is warped, if it is determined that warping occurs;

[0068] S4. If it is determined that edge warping occurs, the image of the blade to be inspected is taken by the fluorescent imaging CCD camera 8 to determine the warping position, the smoothing unit is started, the servo motor 10 and the electromagnetic ring are used to adjust the smoothing direction, and the warped part is smoothed. Then the fluorescent imaging CCD camera 8 switches the working state to obtain the fluorescent imaging data of the blade to be inspected.

[0069] Different from the first implementation, Figure 7It is shown that this embodiment improves the phenomenon that the edge parts of the leaves of plants under drought and pathological conditions are warped, which leads to the overlap of the warped parts when the subsequent fluorescent imaging CCD camera 8 detects the fluorescent imaging inside the leaves. In this embodiment, the surface of the frame member is connected to an air pipe with a valve (which is a prior art, not shown, and the airflow introduced into the air pipe is non-high-speed airflow, so as to avoid excessive impact of the high-speed airflow, which may cause impact damage to the warped part of the leaf during smoothing).

[0070] Specifically, when the fluorescent imaging CCD camera 8 descends, the surface morphology of the blade to be detected is first scanned. Then, when it is determined that there is edge warping on the surface of the blade to be detected, if the warped part is on both sides of the frame, the valve on the surface of the air pipe is directly activated, and the exhausted gas is discharged through the central air pipe 13 and the elastic air pipe 12, which has a smoothing effect on the warped parts on both sides of the frame during the subsequent fluorescent imaging detection process of the fluorescent imaging CCD camera 8 (such as Fig. 9 As shown), when it is necessary to reset later, the magnetic force between the electromagnetic ring and the magnetic plate 11 is switched to repel, which can drive the magnetic plate 11 and the elastic air tube 12 to reset;

[0071] If the warped part is in an asymmetric design, the electromagnetic ring can be started first according to the needs to generate an attraction force between the electromagnetic ring and the magnetic plate 11, so that the magnetic plates 11 are close to each other and drive the elastic air tube 12 to compress, and then the servo motor 10 is started to drive the central air tube 13 to rotate a certain angle to the angle required for smoothing, and then the valve is opened. At this time, since the elastic air tube 12 is in a folded and compressed state, the exhaust airflow is limited and mainly concentrated in the central air tube 13, so that the adjustment of the smoothing airflow direction can be achieved (such as Fig.10 shown).

[0072] In summary, the present application is a box-type multi-environment control in situ real-time photosynthetic phenotype imaging system, which realizes the automatic control of experimental environmental factors (light, temperature, humidity, CO2 concentration), and during the experiment, it realizes the in situ real-time monitoring and imaging of the chlorophyll fluorescence parameters of plants in the box, improves the accuracy of chlorophyll fluorescence parameter detection, improves the efficiency of research work, and cooperates with the adjustment belt 7 to make the detection angle of the fluorescence imaging CCD camera 8 parallel to the leaves to be detected, ensuring the consistency of the detection distance, and cooperates with the smoothing unit to realize the smoothing of the warped part to ensure the accuracy of the detection results.

[0073] In view of current practical needs, the above-mentioned implementation mode adopted in this application is not limited to the scope of protection. Various changes made within the knowledge scope of technical personnel in this field without departing from the concept of this application still fall within the scope of protection of the present invention.

Claims

1. A multi-environment controlled in situ real-time photosynthetic phenotype imaging system, characterized in that: It comprises an environmental control system, a photoelectric control system, an image acquisition system, a plant light system, a computer, a touch screen and a plant sample cultivated in a detection box (1), wherein the environmental control system is used to control the temperature, humidity, CO2 concentration and light index of the detection box (1); The plant light system comprises an LED light board (2) controlled by PWM, and the LED light board (2) is installed on the inner top wall of the detection box (1) to provide plant growth light, measurement light, actinic light and saturation pulse light for plant samples; The image acquisition system comprises a fluorescent imaging CCD camera (8) for photographing the leaf green fluorescent imaging of the plant sample in the detection box (1) and transmitting the photographed image data to a computer. The interior of the detection box (1) is formed into a split chamber and a test chamber by a partition. The plant sample is located in the test chamber. A stepper motor (3) is installed inside the split chamber. The output end of the stepper motor (3) is connected to a lead screw (4) that penetrates into the test chamber. The surface of the lead screw (4) is threadedly sleeved with a moving sleeve, and the end of the moving sleeve that is away from the stepper motor (3) is connected to a rectangular block. The bottom of the moving sleeve is controlled by a motor (9). The output end of the control motor (9) is connected to an electric extension rod (5). The power end of the electric extension rod (5) is connected to a frame member. The frame member is internally rotatably connected to a rotating rod, a driving motor (6) is mounted on the surface of the frame member, and the output end of the driving motor (6) is connected to one end of the rotating rod, an adjusting belt (7) is wound around the surface of the rotating rod, and the end of the adjusting belt (7) is connected to a fluorescent imaging CCD camera (8), a smoothing unit is mounted on the bottom of the frame member, and the smoothing unit includes a servo motor (10) mounted in the middle of the bottom of the frame member, the output end of the servo motor (10) is connected to a central air tube (13) via a short shaft, both ends of the central air tube (13) are connected to elastic air tubes (12), and magnetic plates (11) are mounted on the end surfaces of the two elastic air tubes (12), and an electromagnetic ring is sleeved on the outer surface of the servo motor (10); The photoelectric control system is used to control the plant light system and the environmental control system, and the touch screen is installed on the front of the detection box (1).

2. The multi-environment controlled in situ real-time photosynthetic phenotype imaging system according to claim 1, characterized in that: The plant growth light is composed of red light in the range of 660±5nm, blue light in the range of 450±5nm and infrared light in the range of 730±5nm, which is used to meet the lighting needs of plant growth. The intensity of the measured light is 1μmolm -2 s -1 The actinic light intensity is 400-600 μmol / m -2 s -1 The light energy density of the saturation pulse light is 5000-8000 μmolm -2 s -1 , and the saturation pulse light is realized through laser and homogenizer during operation.

3. The multi-environment controlled in situ real-time photosynthetic phenotype imaging system according to claim 1, characterized in that: The actinic light is realized by modulating pulses, and the on time is 0.2 to 1.5 seconds.

4. The multi-environment controlled in situ real-time photosynthetic phenotype imaging system according to claim 1, characterized in that: A rectangular groove matching the rectangular block is provided inside the partition, the cross-sectional height of the rectangular groove is not less than the sum of the lengths of the moving sleeve, the frame member and the electric extension rod (5) when not started, and the rectangular block penetrates the surface of the lead screw (4).

5. The multi-environment controlled in situ real-time photosynthetic phenotype imaging system according to claim 1, characterized in that: The interior of the adjustment belt (7) is filled with electrorheological fluid, a distance measuring sensor is installed at the bottom of the fluorescent imaging CCD camera (8), and the adjustment belt (7) and the driving motor (6) are both connected to the laser distance measuring sensor limit signal.

6. The multi-environment controlled in situ real-time photosynthetic phenotype imaging system according to claim 1, characterized in that: The surface of the rotating rod is provided with tooth patterns, and the surface of the adjusting belt (7) close to the rotating rod is provided with friction patterns matching the tooth patterns.

7. The multi-environment controlled in situ real-time photosynthetic phenotype imaging system according to claim 1, characterized in that: The working steps of the smoothing unit are as follows: S1, first determine the position of the leaf to be tested on the surface of the plant sample, and then move the frame member downward to above the leaf to be tested with the cooperation of the electric extension rod (5); S2, using an adjustment belt (7) and a drive motor (6) to adjust the fluorescent imaging CCD camera (8) to be parallel to the leaf to be detected; S3, using a fluorescent imaging CCD camera (8) to capture an image of the surface of the blade to be inspected, to detect whether the edge of the blade to be inspected is warped, and if it is determined that warping occurs; S4. If it is determined that edge warping occurs, the image of the blade to be inspected taken by the fluorescent imaging CCD camera (8) is used to determine the warping position, the smoothing unit is started, the smoothing direction is adjusted by using the servo motor (10) and the electromagnetic ring, and the warped part is smoothed. Subsequently, the fluorescent imaging CCD camera (8) switches the working state to obtain the fluorescent imaging data of the blade to be inspected.

8. The multi-environment controlled in situ real-time photosynthetic phenotype imaging system according to claim 1, characterized in that: The elastic air tube (12) is made of an elastic and telescopic material, the central air tube (13) is made of a rigid material, and air outlets are provided on the surfaces of the elastic air tube (12) and the central air tube (13).

9. The multi-environment controlled in situ real-time photosynthetic phenotype imaging system according to claim 1, characterized in that: The horizontal distance between the bottom of the frame member and the adjustment belt (7) is not less than the horizontal length of the central air pipe (13), and the top of the magnetic plate (11) is in close contact with the bottom of the frame member.

Images