Insecticidal composition containing abamectin and flufiprole

Abstract

The invention provides a pesticide composition which comprises abamectin and flufiprole, and the weight ratio of the abamectin to the flufiprole is (1: 10)-(10: 1). The pesticide composition disclosed by the invention has a good control effect on citrus page mites, yellow tea mites, aphids, cabbage caterpillars and the like, the dosage can be reduced, the pesticide application cost is reduced, and the pollution of pesticide residues to the environment is reduced.

Description

Technical Field

[0001] The present invention relates to the field of pesticides, in particular to a pesticide composition and application thereof, and in particular to a pesticide composition containing avermectin and butyronitrile and application thereof. Background Art

[0002] Citrus is one of the important fruits in my country and is widely planted around the world. Field pests are one of the main factors affecting citrus yields. The main pests are tea yellow mites, beet armyworms, aphids, cabbage loopers, etc. Pests can cause great damage during the growth period and cause premature death of plants, which in turn leads to reduced production and poor fruit quality.

[0003] At present, the application of chemical pesticides is an effective means of preventing and controlling plant pests, and different pesticides are often used for different types of plant pests.

[0004] However, the pesticides used to control citrus field pests are used in large quantities. In the pest season, there are many cases of overlapping pests, which require multiple applications. Long-term and large-scale use of a single chemical pesticide to control plant pests is prone to drug resistance, which affects the service life of the pesticide. In addition, there are no pesticides specifically targeting citrus tea yellow mites, beet armyworms, aphids, cabbage worms, etc. Therefore, it is urgent to develop a new type of citrus pesticide that is fast-acting, long-lasting, low-toxic, and safe.

[0005] Avermectin is a highly effective, broad-spectrum antibiotic insecticide and acaricide. It is composed of a group of macrolide compounds, and the active substance is AVERMECTIN, which has stomach poison and contact killing effects on mites and insects. It can be quickly decomposed and dissipated when sprayed on the leaf surface, and the active ingredients that penetrate into the plant parenchyma can exist in the tissue for a long time and have a conductive effect, and have a long residual effect on harmful mites and insects that feed and harm in plant tissues.

[0006] Butyclothion is the trade name of the compound 3-cyano-5-methallylamino-1-(2,6-dichloro-4-trifluoromethylphenyl)-4-trifluoromethylsulfinylpyrazole, and its English trade name is flufiprole, with a molecular formula of C 16 H 10 Cl2F6N4OS, with a relative molecular mass of 491.238 and a CAS number of 704886-18-0, is a non-systemic phenylpyrazole insecticide that targets GABA receptors and is used in rice fields, and performs well in controlling a variety of pests.

[0007] The applicant of the present invention has found through research that the combination of avermectin and butyronitrile has a synergistic effect in a certain ratio. Summary of the invention

[0008] Those skilled in the art know that it is unpredictable whether a pesticide composition can achieve a synergistic effect. The present invention provides a pesticide composition with synergistic effect, wherein the active ingredients of the composition include avermectin and butyronitrile.

[0009] A second object of the present invention is to provide a pesticide formulation containing the pesticide composition.

[0010] The present invention also provides the use of the above pesticide composition and pesticide preparation in preventing and controlling crop pests.

[0011] The beneficial effects of the present invention in application are:

[0012] (1) The compound formulation of avermectin and butyronitrile in the present invention has a synergistic effect, which significantly improves the control effect of pests compared with the use of single-dose avermectin or butyronitrile.

[0013] (2) The abamectin and butyric acid nitrate compound of the present invention can be applied once to control multiple pests that occur simultaneously or successively, thereby reducing the amount of pesticides used, lowering the cost of pesticides, and delaying the increase of pest resistance. DETAILED DESCRIPTION

[0014] To make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention. In addition, in order to better illustrate the present invention, numerous specific details are given in the specific embodiments below. It should be understood by those skilled in the art that the present invention can be implemented equally without certain specific details. In certain embodiments, raw materials, methods, means, etc. well known to those skilled in the art are not described in detail to highlight the purport of the present invention.

[0015] Unless explicitly stated otherwise, throughout the specification and claims, the term "comprise" or variations such as "include" or "comprising" etc., will be understood to include the stated components but not to exclude other components.

[0016] A first aspect of an embodiment of the present invention provides a synergistic pesticide composition, wherein the active ingredients of the pesticide composition include avermectin and butyronitrile;

[0017] Preferably, the weight ratio of avermectin to butyric acid is 1:10-10:1.

[0018] More preferably, the weight ratio of avermectin to butyronitrile is 1:10-1:6 and 1:1-10:1.

[0019] Most preferably, the weight ratio of avermectin to butyronitrile is 1:10, 1:6, 1:1, 2:1, 7:3, 6:1 or 10:1.

[0020] Compared with the use of any one of avermectin and butyric acid nitrile, the pesticide composition of the embodiment of the present invention has a more significant control effect; the use ratio of avermectin and butyric acid nitrile has a synergistic effect in a wider range, which can reduce the amount of medicine used, reduce the cost of application, and reduce the pollution of pesticide residues to the environment. At the same time, in the pesticide composition of the present invention, avermectin and butyric acid nitrile are used in combination according to a specific ratio, with a wide insecticide spectrum, and a significant control effect when multiple pests break out. Compared with the use of any one of avermectin and butyric acid nitrile, a significant synergistic effect can be achieved.

[0021] In order to better exert the performance of the pesticide composition and increase storage stability, the pesticide composition in the embodiment of the present invention is added with a suitable pesticide adjuvant to prepare a pesticide preparation. In the preparation, the relative content of the active ingredient and the pesticide adjuvant can vary within a large range, for example, the mass percentage content of the active ingredient in the preparation is 1 to 30%, preferably 1 to 10%, and more preferably 3 to 5%. Within this preferred range, the active ingredient can be quickly released from the pesticide composition and exert its effect, further improving the prevention and control of field diseases.

[0022] The type of pesticide adjuvant in the embodiment of the present invention can be selected within a wide range, for example, it can include one or more of water, emulsifier, wetting and dispersing agent, wetting agent, dispersant, thickener, antifreeze agent, defoaming agent, and organic solvent.

[0023] The organic solvent is selected from one or more of n-butanol, dimethylacetamide, ethyl acetate, cyclohexanone, aromatic hydrocarbon solvent oil and methyl oleate;

[0024] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, polyoxyethylene styrylphenol ether phosphate, sorbitan trioleate, sorbitan monolaurate, polyoxyethylene fatty alcohol ether and polyoxyethylene castor oil ether;

[0025] The wetting and dispersing agent is selected from one or more of calcium lignin sulfonate, acrylic acid-(meth)acrylate copolymer and sodium lauryl sulfate;

[0026] The dispersant is selected from one or more of polyoxyethylene block copolymers, calcium dodecylbenzene sulfonate, sodium lignin sulfonate, fatty alcohol polyoxyethylene ether, sorbitan polyoxyethylene ether, and styrylphenol polyoxyethylene ether;

[0027] The thickener is selected from one or more of magnesium aluminum silicate, xanthan gum, organic bentonite and white carbon black;

[0028] The antifreeze agent is selected from one or more of ethylene glycol, propylene glycol, glycerol, urea and sodium chloride;

[0029] The defoamer is selected from polyether defoamers and / or silicone defoamers; the binder is selected from one or more of sucrose, maltose, dextrin and starch;

[0030] The solid inert carrier may include one or more of diatomaceous earth, kaolin, bentonite, talc, white carbon black and light calcium.

[0031] The pesticide formulations of the embodiments of the present invention can be prepared into various dosage forms, for example, suspensions, microcapsule suspensions, emulsifiable concentrates, oil suspensions, microemulsions, aqueous emulsions, aqueous solutions, wettable powders, tablets and water dispersible granules. The above-mentioned various dosage forms can be prepared by preparation methods known in the art, which will not be described in detail here.

[0032] The pesticide composition of the embodiment of the present invention can be provided in the form of a finished product, that is, the components in the composition have been mixed; or it can be provided in the form of a separate preparation of each component, which only needs to be mixed evenly before use.

[0033] The second aspect of the embodiment of the present invention provides the use of the above pesticide composition in preventing and controlling field diseases of crops. The crops are citrus, and the pests include leaf mites, tea yellow mites, etc.

[0034] The above pesticide composition is used for field pest control, especially in the pest season, when multiple pests break out, the control effect is remarkable, the cost of application is reduced, the environmental pollution caused by pesticide residues is reduced, one drug can treat multiple diseases, the labor force is reduced, and the generation of drug resistance is delayed. The above pesticide composition provides a new choice for field diseases.

[0035] The specific embodiments of the present invention are described in detail below. It should be understood that the examples of the specific embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0036] Example 1

[0037] Preparation of 1% avermectin·10% butyronitrile SC (1:10)

[0038] Calculated by mass percentage, the distribution ratio of each group is 1% avermectin, 10% butyronitrile, 3% phosphate, 3% carboxylate, 0.1% xanthan gum, 0.2% silicone defoamer, 0.2% kason, 5% urea, and the balance is made up with water.

[0039] Example 2

[0040] Preparation of 1% avermectin·6% butyronitrile SC (1:6)

[0041] Calculated by mass percentage, the distribution ratio of each group is 1% avermectin, 6% butyronitrile, 3% phosphate, 3% carboxylate, 0.1% xanthan gum, 0.2% silicone defoamer, 0.2% kason, 5% urea, and the balance is made up with water.

[0042] Example 3

[0043] Preparation of 1% Avermectin·1% Butyronitrile WP (1:1)

[0044] Calculated by mass percentage, the distribution ratio of each group is 1% avermectin, 3% butyronitrile, 8% sodium lignin sulfonate, 4% carboxylate, and kaolin makes up the balance.

[0045] Example 4

[0046] Preparation of 2% Avermectin·1% Butyronitrile EC (2:1)

[0047] The distribution ratio of each group is 2% avermectin, 1% butyronitrile, 20% cyclohexanone, 6% polyoxyethylene polyoxypropylene block copolymer, 5% calcium dodecylbenzene sulfonate, and xylene is added, and the mixture is stirred evenly.

[0048] Example 5

[0049] Preparation of 7% Avermectin·3% ​​Butyronitrile EC (7:3)

[0050] The distribution ratio of each group is 2% avermectin, 1% butyronitrile, 30% cyclohexanone, 6% polyoxyethylene polyoxypropylene block copolymer, 5% calcium dodecylbenzene sulfonate, and xylene is supplemented, and the mixture is stirred evenly.

[0051] Example 6

[0052] Preparation of 6% Avermectin·1% Butyronitrile EC (6:1)

[0053] The distribution ratio of each group is 2% avermectin, 1% butyronitrile, 20% cyclohexanone, 6% polyoxyethylene polyoxypropylene block copolymer, 5% calcium dodecylbenzene sulfonate, and xylene is added, and the mixture is stirred evenly.

[0054] Example 7

[0055] Preparation of 10% Avermectin·1% Butyronitrile WP (10:1)

[0056] Calculated by mass percentage, the distribution ratio of each group is 10% avermectin, 1% butyronitrile, 8% sodium lignin sulfonate, 4% carboxylate, and kaolin makes up the balance.

[0057] Comparative Example 1

[0058] Preparation of 5% Avermectin SC

[0059] Calculated by mass percentage, the distribution ratio of each group is 5% avermectin, 3% phosphate, 2% carboxylate, 0.1% xanthan gum, 0.3% silicone defoamer, 0.2% kason, 5% ethylene glycol, and the balance is made up with water.

[0060] Comparative Example 2

[0061] Preparation of 5% butyronitrile SC

[0062] Calculated by mass percentage, the distribution ratio of each group is 5% butyric acid nitrile, 3% phosphate, 2% carboxylate, 0.1% xanthan gum, 0.3% silicone defoamer, 0.2% kason, 5% ethylene glycol, and the balance is made up with water.

[0063] Comparative Example 3

[0064] Preparation of 1% Avermectin·5% Butyronitrile WP (1:5)

[0065] Calculated by mass percentage, the distribution ratio of each group is 1% avermectin, 5% butyronitrile, 8% sodium lignin sulfonate, 4% carboxylate, and kaolin makes up the balance.

[0066] Comparative Example 4

[0067] Preparation of 1% avermectin·4% butyronitrile SC (1:4)

[0068] Calculated by mass percentage, the distribution ratio of each group is 1% avermectin, 4% butyronitrile, 3% phosphate, 3% carboxylate, 0.1% xanthan gum, 0.2% silicone defoamer, 0.2% kason, 5% urea, and the balance is made up with water.

[0069] Comparative Example 5

[0070] Preparation of 1% Avermectin·3% ​​Butyronitrile WP (1:3)

[0071] Calculated by mass percentage, the distribution ratio of each group is 1% avermectin, 3% butyronitrile, 8% sodium lignin sulfonate, 4% carboxylate, and kaolin makes up the balance.

[0072] The inventors conducted a study on the synergistic effect of compounding an insecticidal composition of avermectin and butyric acid nitrate. The experiment used citrus tea yellow mite as the test object. Unless otherwise specified, the ratios (including percentages) mentioned below are all weight ratios. The specific method is as follows:

[0073] 1. Indoor bio-test screening

[0074] 1.1 Test method:

[0075] The indoor toxicity determination was carried out according to the Guidelines for Indoor Bioassay Tests of Pesticides Part 14: Leaf Dipping Method (NY / T1154.14-2008). The determination steps are as follows: immerse fresh citrus leaves in the solution for 5 seconds, take out and absorb the excess solution with absorbent paper, dry naturally, place in a culture dish with a diameter of 7 cm, and inoculate about 50 adult citrus tea yellow mites, seal with plastic wrap (pierce several holes with an insect pin), and repeat each concentration treatment and control treatment 4 times. After the treatment, each culture dish was placed in an insect breeding room with a temperature of (25±1)℃, a relative humidity of (60±10)%, and a light intensity of L:D=14h:10h.

[0076] 1.2 Data statistics and analysis:

[0077] 48 hours after treatment, check the death of the test insects and calculate the mortality rate (the criterion for judging the death of the test insects is the absence of spontaneous reaction when the test insects are lightly pricked with a needle), and record the total number of insects and the number of dead insects.

[0078] According to the survey data, the corrected mortality of each treatment was calculated, and the co-toxicity coefficient (CTC value) of the mixture was calculated using the Sun Yunpei method in accordance with NY / T1154.7-2006.

[0079] The adjusted mortality rate is calculated according to formula (1) and formula (2) in percentage (%), and the calculation result is rounded to two decimal places:

[0080]

[0081] Where:

[0082] P1---mortality rate;

[0083] K---Number of dead insects;

[0084] N---Total number of insects treated.

[0085]

[0086] Where:

[0087] P2---adjusted mortality rate;

[0088] Pt---treatment mortality;

[0089] P0---blank control mortality rate.

[0090] If the control mortality rate is less than 5%, no correction is made; if the control mortality rate is between 5% and 20%, correction is made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0091] The logarithm of the drug concentration (mg / L) was used as the independent variable X, and the probability value of the corrected mortality rate was used as the dependent variable Y. The toxicity regression equations were established respectively, and the LC50 of the single agent and the mixture of each ratio and the co-toxicity coefficient of the mixture were calculated using DPS software to compare the synergy. The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method. The co-toxicity coefficient CTC is calculated as follows:

[0092] (A is avermectin as the standard agent, its toxicity index is 100, B is butyronitrile): Use DPS statistical analysis software to perform statistical analysis, calculate the LC50 of each agent, and calculate the co-toxicity coefficient (CTC value) of the mixture according to Sun Yunpei's method.

[0093] Measured toxicity index (ATI) = (LC50 of standard agent / LC50 of test agent) × 100.

[0094] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture.

[0095] Co-toxicity coefficient (CTC) = [actual toxicity index (ATI) of the mixture / theoretical toxicity index (TTI) of the mixture] × 100.

[0096] According to the NY / T11547.7-2006 pesticide joint action classification standard: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect; and a co-toxicity coefficient (CTC) 80 < 120 indicates an additive effect. The results are shown in Table 1.

[0097] Table 1 Indoor combined toxicity test of avermectin and butyric acid nitrate against citrus tea yellow mite

[0098]

[0099]

[0100] As can be seen from Table 1, when the mixing ratio of avermectin and butyric acid nitrate is in the range of 1:10-1:6 and 1:1-10:1 (mass ratio), the co-toxicity coefficient (CTC) is 122.94-159.11, which shows a synergistic effect on citrus tea yellow mite; however, when the mixing ratio is in the range of 1:5-1:3 (mass ratio), the co-toxicity coefficient (CTC) is 73.48-77.84, which shows an antagonistic effect on citrus tea yellow mite. This shows that the combination of avermectin and butyric acid nitrate is reasonable and feasible within a certain range.

[0101] 2. Application test example 1

[0102] The effects of the pesticide compositions of Examples 1-7 and Comparative Examples 1-5 on citrus pests were tested in citrus planting fields. The specific steps included spraying the pesticide compositions of Examples 1-6 and Comparative Examples 1-5 in different planting areas. The dosage of the active ingredient was 1.0 g / mu. The planting area of ​​each plant tested was 40 m2. 2 , diluted with water to a spray volume of 40L per mu during application, and water was used to replace the pesticide composition as a blank treatment. Except for the application of the pesticide composition according to the above scheme, the other planting conditions in each planting area were exactly the same. Three days after spraying the pesticide composition, the control effect of citrus tea yellow mites in the planting area was tested. The specific test method was carried out in accordance with the "Guidelines for Field Efficacy Tests of Pesticides". Random sampling was performed at 5 points in each treatment area, and 10 citrus trees were investigated in each treatment area. The control effect of citrus tea yellow mites in each treatment area was investigated. The mortality rate was calculated according to formula (4) and the corrected mortality rate was calculated according to formula (5). The specific results are shown in Table 2:

[0103]

[0104]

[0105] Table 2

[0106]

[0107]

[0108] It can be seen from the above table that the two-component compound pesticide composition containing abamectin and butyronitrile in the embodiment of the present invention is more effective in controlling insect pests of citrus than single-component abamectin and butyronitrile; both abamectin and butyronitrile have a synergistic effect within a wider range of dosage ratios, but show antagonism in the range of 1:5 to 1:3, and the effect is reduced. The pesticide composition disclosed in the present invention further improves the effect of controlling diseases of citrus.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A pesticide composition, characterized in that: The active ingredients include avermectin and butyronitrile, and the weight ratio of avermectin to butyronitrile is 1:10-10:

1.

2. The pesticide composition according to claim 1, characterized in that The weight ratio of avermectin to butyric acid nitrite is 1:10-1:6 and 1:1-10:

1.

3. The pesticide composition according to claim 2, characterized in that The weight ratio of avermectin to butyric acid nitrite is 1:10, 1:6, 1:1, 2:1, 7:3, 6:1 or 10:

1.

4. A pesticide preparation, characterized in that: The pesticide composition comprises the pesticide composition according to any one of claims 1 to 3 and a suitable pesticide adjuvant, preferably, the mass percentage content of the active ingredient in the preparation is 1 to 30%, more preferably 1 to 10%, and most preferably 3 to 5%.

5. The pesticide formulation according to claim 4, characterized in that: The pesticide adjuvant is one or more of a dispersant, an emulsifier, a solvent, a thickener, a stabilizer, a defoamer, an antifreeze agent, a preservative and water.

6. The preparation according to claim 4 or 5, characterized in that: The invention can be prepared into a suitable dosage form, which is one or more of a suspension concentrate, a seed treatment suspension concentrate, an emulsifiable concentrate, a microcapsule suspension concentrate, an aqueous emulsion, and a microemulsion.

7. Use of the pesticide composition according to any one of claims 1 to 3 or the pesticide preparation according to any one of claims 4 to 6 in preventing and controlling field insect pests of crops, preferably, the crops are citrus.

8. The use according to claim 7, characterized in that: The insect pests are one or more of leaf mites and tea yellow mites.

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