Method for separating and detecting ginsenoside in ginseng

Abstract

The invention discloses a method for separating and detecting ginsenoside in ginseng, and relates to the technical field of ginsenoside extraction.The method for separating and detecting ginsenoside in ginseng specifically comprises the following steps that 1, ginseng with ginsenoside needing to be separated is selected and cleaned, glabrous greenbrier rhizome is cleaned and sliced, and a ginseng extract is obtained; then the ginseng is fully dried through drying equipment, impurities on the surface of the ginseng are removed, and the cleanliness of the ginseng is guaranteed; step (2), performing enzymolysis on the selected ginseng; according to the method for separating and detecting the ginsenoside in the ginseng, when the ginsenoside in the ginseng is separated and extracted, a ginseng sample is firstly subjected to enzymolysis, and in the enzymolysis process, cellulase can decompose components of ginseng cell walls and destroy ginseng cell structures, so that proteins in cells are released; impurities such as protein and polysaccharide in the ginseng are removed, so that the purity and content of the ginsenoside extracted subsequently can be improved, and the extraction rate of the ginsenoside is increased.

Description

Technical Field

[0001] The invention relates to the technical field of ginsenoside extraction, in particular to a method for separating and detecting ginsenoside in ginseng. Background Art

[0002] Ginseng is a perennial herbaceous plant of the genus Panax. Its fleshy root is widely used in traditional Chinese medicine. It has the effects of greatly replenishing vitality, restoring pulse and strengthening deficiency, nourishing spleen and lung, promoting body fluid and quenching thirst, calming the mind and improving intelligence. It is suitable for adjusting blood pressure, restoring heart function, treating symptoms such as neurasthenia and physical weakness. Ginseng also has the effects of expectorant, stomachic, diuretic, and excitement. Ginseng can be made into red ginseng, ginseng, sugar ginseng and other forms through different processing methods. Its usage includes making tea, stewing soup, making pills, etc. It should be noted that patients with excess syndrome and heat syndrome should avoid taking ginseng, and it is not advisable to drink tea when taking ginseng, so as not to affect the efficacy of the medicine. At the same time, there are a large number of ginsenosides in ginseng, and the ginsenosides in ginseng can be extracted for pharmaceutical production.

[0003] Ginsenoside is an active ingredient extracted from ginseng, which has many significant effects and functions. First, it is outstanding in enhancing immunity, which can improve the body's resistance and help prevent diseases. Secondly, ginsenoside has anti-fatigue effects, can improve physical strength and endurance, and is especially suitable for people who need high-intensity physical and mental work. In addition, it also has a positive impact on cardiovascular health, helping to lower blood pressure and improve blood circulation.

[0004] In terms of anti-oxidation, ginsenosides can remove free radicals, slow down the aging process, and promote cell repair and regeneration. Studies have also found that ginsenosides may have certain effects on fighting tumors, regulating blood sugar, and improving liver function. Therefore, although ginseng itself has certain health benefits, ginsenosides are more widely used in the health care field due to their concentrated active ingredients and stronger biological activity, making them an important ingredient in modern medicine and health products.

[0005] For example, the method for preparing a ginsenoside extract for beverages with application number 201910761154.5 first extracts the ginsenosides in the raw material ginseng in a liquid state, and uses diatomaceous earth to remove the oil in the ginsenoside extraction filtrate, and then passes it through a three-stage filtration system to filter out insoluble tiny impurities, and finally spray-drying is performed. The unstable macromolecular protein is separated by combining the difference between the molecular weight of protein and the molecular weight of ginsenoside and ultrafiltration membrane separation, thereby obtaining a ginsenoside extract with high solution stability. The ginsenoside extract obtained by this method can form a clarified system with the solution, thereby ensuring that the obtained ginsenoside extract has very good solution stability, greatly improving the application concentration and stability of ginsenosides in beverages. However, this method still has certain defects; In the extraction of ginsenosides, there is no method for improving the extraction rate of ginsenosides, which results in a low extraction rate and content of ginsenosides during the extraction process.

[0006] Therefore, we proposed a method for separating and detecting ginsenosides in ginseng in order to solve the above problems. Summary of the invention

[0007] The purpose of the present invention is to provide a method for separating and detecting ginsenosides in ginseng, so as to solve the problem raised by the above background technology that there is no method for improving the extraction rate of ginsenosides in the extraction of ginsenosides in the current market, resulting in a low extraction rate and content of ginsenosides in the extraction process.

[0008] To achieve the above object, the present invention provides the following technical solution: a method for separating and detecting ginsenosides in ginseng, the method for separating and detecting ginsenosides in ginseng specifically comprising the following steps: Step (1), selecting ginseng from which ginsenosides need to be separated, washing the ginseng, washing the ginseng and slicing the ginseng, and then fully drying the ginseng through a drying device to remove impurities on the surface of the ginseng to ensure the cleanliness of the ginseng; Step (2), enzymolyzing the selected ginseng, using cellulase during enzymolysis, placing the ginseng slices in an enzymolysis tank, then adding 2% cellulase to the enzymolysis tank, heating the enzymolysis tank, allowing the cellulase and ginseng slices in the enzymolysis tank to react at a temperature of 45° C., and the reaction time is between 1 hour and 2 hours; Step (3), after the ginseng is hydrolyzed by cellulase, the liquid and solid in the hydrolysis tank are separated to obtain a hydrolysis liquid and a hydrolysis solid, the hydrolysis liquid and the hydrolysis solid are separated, the hydrolysis solid is chopped in one step, and then the hydrolysis solid is placed in an aqueous solution, the aqueous solution is heated to 60°C-80°C, and the hydrolysis solid is soaked and extracted for 2 hours-3 hours; In step (4) and step (3), the enzymatic hydrolysis solid extract is obtained, the enzymatic hydrolysis solid extract is filtered, and impurities in the extract are filtered to separate the extract from the impurities, thereby obtaining a relatively pure enzymatic hydrolysis solid extract; Step (5), placing the enzymatic solid extract and the enzymatic hydrolysis solution in an extraction tank at the same time, adding ethanol solvent to the extraction tank at the same time, accelerating the dissolution of ginsenosides in the enzymatic solid extract and the enzymatic hydrolysis solution by the ethanol solvent, thereby forming a ginsenoside extract, and recycling the ginsenoside extract 2-3 times by a reflux extraction method; Step (6), concentrating the ginsenoside extract, and drying the ginsenoside extract by vacuum drying to remove moisture. During vacuum drying, the interior of the dryer is in a negative pressure state. When the water content of the ginsenoside extract meets the standard requirements, the drying is stopped and the dried ginsenoside extract is taken out; Step (7), detecting ginsenosides in the ginsenoside sample by ultraviolet spectrophotometry, taking a small amount of sample from the ginsenoside extract, weighing and recording the ginsenoside sample, and then reflux extracting and soaking the ginsenoside sample for 6 hours to 8 hours, and then evaporating it in a water bath, extracting and diluting it after evaporation, establishing a standard curve, and measuring it on the machine.

[0009] In the step (1), when selecting ginseng for separation, the root of ginseng is selected for cutting and cleaning. Ginsenosides are mainly present in the root of ginseng, thereby increasing the subsequent extraction amount of ginsenosides.

[0010] In the step (2), during the enzymatic hydrolysis process, cellulase decomposes the components of the ginseng cell wall and destroys the ginseng cell structure, thereby releasing the protein in the cell and removing impurities such as protein and polysaccharides in ginseng, thereby improving the purity and content of ginsenosides extracted subsequently and improving the extraction rate of ginsenosides.

[0011] In the step (3), an alkaline extraction solvent is simultaneously added to the enzymatically hydrolyzed solid to further improve the extraction rate of ginsenosides.

[0012] The enzymatically hydrolyzed solid extract in step (4) can be extracted again to extract the ginsenosides in the enzymatically hydrolyzed solid extract, thereby making the extraction of ginsenosides more thorough.

[0013] In the step (5), the ginsenoside extract is subjected to multiple cycles of extraction, thereby improving the purity of the ginsenoside extract and the extraction rate of the ginsenoside.

[0014] In the step (6), the dried ginsenoside extract can be tableted or granulated according to actual needs.

[0015] In step (6), the drying of the ginsenoside extract is carried out under vacuum conditions. The boiling point of the solvent can be reduced by lowering the pressure, thereby accelerating the drying process of the ginsenoside extract. The vacuum drying method is suitable for heat-sensitive substances, thereby avoiding the destruction of ginsenosides by high temperature.

[0016] In the step (7), the ginsenosides in the ginsenoside sample are detected without filtering the ginsenoside sample.

[0017] Compared with the prior art, the invention has the following beneficial effects: the separation and detection method of ginsenosides in ginseng: When separating and extracting ginsenosides from ginseng, the ginseng sample is first enzymatically hydrolyzed. During the enzymatic hydrolysis process, cellulase will decompose the components of the ginseng cell wall and destroy the ginseng cell structure, thereby releasing the protein in the cell and removing impurities such as protein and polysaccharides from ginseng, thereby improving the purity and content of the subsequently extracted ginsenosides and improving the extraction rate of ginsenosides; Placing the enzymatic solid extract and the enzymatic hydrolysis solution in an extraction tank at the same time, adding an ethanol solvent to the extraction tank at the same time, accelerating the dissolution of ginsenosides in the enzymatic solid extract and the enzymatic hydrolysis solution by the ethanol solvent, thereby forming a ginsenoside extract, and recycling the ginsenoside extract 2-3 times by a reflux extraction method, and recycling the ginsenoside extract for multiple times, thereby improving the purity of the ginsenoside extract and the extraction rate of the ginsenoside; The extraction of ginsenosides is accelerated by using ethanol solvent for dissolution assistance. The extraction method is simple and convenient and does not affect the extraction rate of ginsenosides. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the work flow structure of the present invention; Figure 2 This is a graph showing the test results of ginsenoside extraction before and after enzymatic hydrolysis of ginseng in the present invention. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] See also Figure 1-Figure 2 The present invention provides a technical solution: a method for separating and detecting ginsenosides in ginseng, the method for separating and detecting ginsenosides in ginseng specifically comprises the following steps: Step (1), selecting ginseng from which ginsenosides need to be separated, washing the ginseng, washing the ginseng and slicing the ginseng, and then fully drying the ginseng through a drying device to remove impurities on the surface of the ginseng to ensure the cleanliness of the ginseng; Step (2), enzymolyzing the selected ginseng, using cellulase during enzymolysis, placing the ginseng slices in an enzymolysis tank, then adding 2% cellulase to the enzymolysis tank, heating the enzymolysis tank, allowing the cellulase and ginseng slices in the enzymolysis tank to react at a temperature of 45° C., and the reaction time is between 1 hour and 2 hours; Step (3), after the ginseng is hydrolyzed by cellulase, the liquid and solid in the hydrolysis tank are separated to obtain a hydrolysis liquid and a hydrolysis solid, the hydrolysis liquid and the hydrolysis solid are separated, the hydrolysis solid is chopped in one step, and then the hydrolysis solid is placed in an aqueous solution, the aqueous solution is heated to 60°C-80°C, and the hydrolysis solid is soaked and extracted for 2 hours-3 hours; In step (4) and step (3), the enzymatic hydrolysis solid extract is obtained, the enzymatic hydrolysis solid extract is filtered, and impurities in the extract are filtered to separate the extract from the impurities, thereby obtaining a relatively pure enzymatic hydrolysis solid extract; Step (5), placing the enzymatic solid extract and the enzymatic hydrolysis solution in an extraction tank at the same time, adding ethanol solvent to the extraction tank at the same time, accelerating the dissolution of ginsenosides in the enzymatic solid extract and the enzymatic hydrolysis solution by the ethanol solvent, thereby forming a ginsenoside extract, and recycling the ginsenoside extract 2-3 times by a reflux extraction method; Step (6), concentrating the ginsenoside extract, and drying the ginsenoside extract by vacuum drying to remove moisture. During vacuum drying, the interior of the dryer is in a negative pressure state. When the water content of the ginsenoside extract meets the standard requirements, the drying is stopped and the dried ginsenoside extract is taken out; Step (7), detecting ginsenosides in the ginsenoside sample by ultraviolet spectrophotometry, taking a small amount of sample from the ginsenoside extract, weighing and recording the ginsenoside sample, and then reflux extracting and soaking the ginsenoside sample for 6 hours to 8 hours, and then evaporating it in a water bath, extracting and diluting it after evaporation, establishing a standard curve, and measuring it on the machine.

[0021] In step (1), when selecting ginseng for separation, the root of ginseng is selected for cutting and cleaning. Ginsenosides are mainly present in the root of ginseng, thereby increasing the subsequent extraction amount of ginsenosides.

[0022] In step (2), during the enzymatic hydrolysis process, cellulase will decompose the components of the ginseng cell wall and destroy the ginseng cell structure, thereby releasing the protein in the cell and removing impurities such as protein and polysaccharides in ginseng, thereby improving the purity and content of the subsequently extracted ginsenosides and improving the extraction rate of ginsenosides.

[0023] In step (3), an alkaline extraction solvent is simultaneously added to the enzymatically hydrolyzed solid to further improve the extraction rate of ginsenosides.

[0024] The enzymatically hydrolyzed solid extract in step (4) can be extracted again to extract the ginsenosides in the enzymatically hydrolyzed solid extract, thereby making the extraction of ginsenosides more thorough.

[0025] In step (5), the ginsenoside extract is subjected to multiple cycles of extraction, thereby improving the purity of the ginsenoside extract and the extraction rate of ginsenoside.

[0026] In step (6), the dried ginsenoside extract can be tableted or granulated according to actual needs.

[0027] In step (6), the drying of the ginsenoside extract is carried out under vacuum conditions. The boiling point of the solvent can be reduced by lowering the pressure, thereby accelerating the drying process of the ginsenoside extract. The vacuum drying method is suitable for heat-sensitive substances, thereby avoiding the destruction of ginsenosides by high temperature.

[0028] In step (7), ginsenosides in the ginsenoside sample are detected without filtering the ginsenoside sample.

[0029] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0030] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for separating and detecting ginsenosides in ginseng, characterized in that: The separation and detection method of ginsenosides in ginseng specifically comprises the following steps: Step (1), selecting ginseng from which ginsenosides need to be separated, washing the ginseng, washing the ginseng and slicing the ginseng, and then fully drying the ginseng through a drying device to remove impurities on the surface of the ginseng to ensure the cleanliness of the ginseng; Step (2), enzymolyzing the selected ginseng, using cellulase during enzymolysis, placing the ginseng slices in an enzymolysis tank, then adding 2% cellulase to the enzymolysis tank, heating the enzymolysis tank, allowing the cellulase and ginseng slices in the enzymolysis tank to react at a temperature of 45° C., and the reaction time is between 1 hour and 2 hours; Step (3), after the ginseng is hydrolyzed by cellulase, the liquid and solid in the hydrolysis tank are separated to obtain a hydrolysis liquid and a hydrolysis solid, the hydrolysis liquid and the hydrolysis solid are separated, the hydrolysis solid is chopped in one step, and then the hydrolysis solid is placed in an aqueous solution, the aqueous solution is heated to 60°C-80°C, and the hydrolysis solid is soaked and extracted for 2 hours-3 hours; In step (4) and step (3), the enzymatic hydrolysis solid extract is obtained, the enzymatic hydrolysis solid extract is filtered, and impurities in the extract are filtered to separate the extract from the impurities, thereby obtaining a relatively pure enzymatic hydrolysis solid extract; Step (5), placing the enzymatic solid extract and the enzymatic hydrolysis solution in an extraction tank at the same time, adding ethanol solvent to the extraction tank at the same time, accelerating the dissolution of ginsenosides in the enzymatic solid extract and the enzymatic hydrolysis solution by the ethanol solvent, thereby forming a ginsenoside extract, and recycling the ginsenoside extract 2-3 times by a reflux extraction method; Step (6), concentrating the ginsenoside extract, and drying the ginsenoside extract by vacuum drying to remove moisture. During vacuum drying, the interior of the dryer is in a negative pressure state. When the water content of the ginsenoside extract meets the standard requirements, the drying is stopped and the dried ginsenoside extract is taken out; Step (7), detecting ginsenosides in the ginsenoside sample by ultraviolet spectrophotometry, taking a small amount of sample from the ginsenoside extract, weighing and recording the ginsenoside sample, and then reflux extracting and soaking the ginsenoside sample for 6 hours to 8 hours, and then evaporating it in a water bath, extracting and diluting it after evaporation, establishing a standard curve, and measuring it on the machine.

2. The method for separating and detecting ginsenosides in ginseng according to claim 1, characterized in that: In the step (1), when selecting ginseng for separation, the root of ginseng is selected for cutting and cleaning. Ginsenosides are mainly present in the root of ginseng, thereby increasing the subsequent extraction amount of ginsenosides.

3. The method for separating and detecting ginsenosides in ginseng according to claim 1, characterized in that: In the step (2), during the enzymatic hydrolysis process, cellulase decomposes the components of the ginseng cell wall and destroys the ginseng cell structure, thereby releasing the protein in the cell and removing impurities such as protein and polysaccharides in ginseng, thereby improving the purity and content of ginsenosides extracted subsequently and improving the extraction rate of ginsenosides.

4. The method for separating and detecting ginsenosides in ginseng according to claim 1, characterized in that: In the step (3), an alkaline extraction solvent is simultaneously added to the enzymatically hydrolyzed solid to further improve the extraction rate of ginsenosides.

5. The method for separating and detecting ginsenosides in ginseng according to claim 1, characterized in that: The enzymatically hydrolyzed solid extract in step (4) can be extracted again to extract the ginsenosides in the enzymatically hydrolyzed solid extract, thereby making the extraction of ginsenosides more thorough.

6. The method for separating and detecting ginsenosides in ginseng according to claim 1, characterized in that: In the step (5), the ginsenoside extract is subjected to multiple cycles of extraction, thereby improving the purity of the ginsenoside extract and the extraction rate of the ginsenoside.

7. The method for separating and detecting ginsenosides in ginseng according to claim 1, characterized in that: In the step (6), the dried ginsenoside extract can be tableted or granulated according to actual needs.

8. The method for separating and detecting ginsenosides in ginseng according to claim 1, characterized in that: In step (6), the drying of the ginsenoside extract is carried out under vacuum conditions. The boiling point of the solvent can be reduced by lowering the pressure, thereby accelerating the drying process of the ginsenoside extract. The vacuum drying method is suitable for heat-sensitive substances, thereby avoiding the destruction of ginsenosides by high temperature.

9. The method for separating and detecting ginsenosides in ginseng according to claim 1, characterized in that: In the step (7), the ginsenosides in the ginsenoside sample are detected without filtering the ginsenoside sample.

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