Plant incubator for plant abiotic stress experiment

Abstract

The invention discloses a plant incubator for plant abiotic stress experiments, which belongs to the technical field of abiotic stress experiments and comprises a casing, a group of storage plates fixedly connected with the inner wall of the casing, a group of transparent cover plates arranged on the front side of the casing and rotating plates rotationally connected with the upper surfaces of the storage plates. The upper surface of the rotating plate is fixedly connected with a group of culture dishes, and the left side surface of the machine shell is fixedly connected with a group of glass tubes. According to the device, a proper amount of culture solution is added into the glass tube, the absorbed culture solution is different along with the difference of the growth conditions of plants, the influence of external factors on the plants can be judged by observing the balance of the culture solution in the glass tube, and the growth conditions of the plants in the culture dishes in different groups are observed in a matched manner; the influence of the abiotic outside world on plant growth can be more visually judged, meanwhile, the device can synchronously carry out experiments of a plurality of external factor control variables, and the time needed by abiotic stress experiments is shortened.

Description

Technical Field

[0001] The invention belongs to the technical field of plant abiotic stress experiments, and in particular relates to a plant culture device used for plant abiotic stress experiments. Background Art

[0002] The stress caused to plants by changes in external natural conditions are non-biological environmental conditions that are not conducive to plant survival, growth and development, and may even cause injury, destruction and death, including low temperature, high temperature, drought, lack of oxygen, strong winds, damage, and air, soil or water pollution. Plant stress resistance refers to certain traits that plants have to resist adverse environments, such as cold resistance, drought resistance, salt resistance, disease and pest resistance, etc.

[0003] Plant abiotic stress experiments can be used as an important means to cultivate excellent stress-resistant plants. In plant abiotic stress experiments, a variety of plant species are used, aiming to explore the response mechanisms and adaptation strategies of different plants to abiotic stress. Simple abiotic stress experiments are to determine whether they have an impact on plant growth by changing external abiotic factors. When an impact occurs, the response mechanisms and adaptation strategies of plants to external factors are explored.

[0004] The growth of plants is a slow process, which makes it difficult to intuitively judge the growth changes of plants. The method of directly observing and recording through observation holes becomes more time-consuming and laborious. For example, the patent with authorization number CN 216123647U discloses a plant culture device for plant abiotic stress experiments, including a tank body and a tank cover. The tank body is filled with fillers for fixing plant roots. The top of the tank body is open, and the tank cover can be installed at the opening and forms a detachable fixed connection with the tank body. The tank cover is provided with plant cultivation holes, monitoring holes and feeding holes. The plant cultivation holes are used to plant plants, the monitoring holes are used to monitor the physical and chemical properties of the culture solution in the tank body, and the feeding holes are used to fill the tank body with nutrient solution and stress substances. The plant growth is directly observed to judge the influence of abiotic external factors on plants. In order to judge which abiotic external factors affect plants, the experiment is carried out by controlling variables. However, the variables of abiotic external factors are diverse, which makes the abiotic stress experiment take longer. Summary of the invention

[0005] The purpose of the present invention is to solve the problem that it is difficult for existing incubators to intuitively judge the effects of non-biological external factors on plant growth and the control variables, resulting in a long experiment time, and to propose a plant incubator for plant abiotic stress experiments.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A plant culture device for abiotic stress experiments on plants comprises a casing, a group of storage plates fixedly connected to the inner wall of the casing, a group of transparent cover plates arranged on the front of the casing and a rotating plate rotatably connected to the upper surface of each storage plate, a group of culture dishes fixedly connected to the upper surface of the rotating plate, a group of glass tubes fixedly connected to the left side of the casing, a transfer tube embedded in the upper surface of the transfer plate is connected to the inside of the glass tube through a water pipe, a group of absorbent cotton strips are fixedly connected to the upper surface of a cotton block rotatably connected to the inner wall of the transfer tube, each of the absorbent cotton strips extends to the inside of a culture dish, a heating rod, an ultraviolet lamp and a humidifier are fixedly connected to the middle of the upper surfaces of the three transfer plates, and a fan is fixedly connected to the lower part of the right side of the casing.

[0008] As a further description of the above technical solution:

[0009] A group of through tubes are fixedly connected to the upper surface of the cotton block, the upper ends of the through tubes are fixedly connected to the bottom surface of the culture dish, and the absorbent cotton strips penetrate the through tubes and extend to the interior of the culture dish.

[0010] As a further description of the above technical solution:

[0011] A gear ring is fixedly connected to the outer surface of the rotating plate, a motor is embedded on the right side of the upper surface of the storage plate, and a gear meshing with the gear ring is fixedly connected to the output end of the motor.

[0012] As a further description of the above technical solution:

[0013] The right side of the housing is fixedly connected with a first cover plate, the middle of the right side of the first cover plate is fixedly connected with a carbon dioxide generator, and a round hole is opened on the left side of the first cover plate.

[0014] As a further description of the above technical solution:

[0015] The left side of the casing is fixedly connected with a second cover plate, the middle part of the left side of the second cover plate is fixedly connected with an induced draft fan, and the air outlet end of the induced draft fan is fixedly connected with an air collecting pipe.

[0016] As a further description of the above technical solution:

[0017] A set of one-way valves is arranged at the fixed connection point between the second cover plate and the casing, the air inlet end of the one-way valve is connected to the interior of the casing, and the air outlet end of the one-way valve is connected to the interior of the second cover plate.

[0018] As a further description of the above technical solution:

[0019] A bracket is fixedly connected to the upper portion of the left side of the second cover plate, a culture bottle is clamped on the upper surface of the bracket, and an illuminating lamp is fixedly connected to the inner top wall of the housing and the bottom surface of the storage plate.

[0020] As a further description of the above technical solution:

[0021] A rubber plug is clamped on the upper surface of the glass tube, a group of through holes connected to the glass tube are opened on the upper surface of the rubber plug, and a slide plate is slidably connected to the inner wall of the slide groove opened on the left side of the rubber plug.

[0022] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0023] In the present invention, by adding a proper amount of culture solution to the inside of the glass tube, the plants to be cultured are placed inside each culture dish. The culture solution absorbed by the plants varies with their growth conditions. The influence of external factors on the plants can be judged by observing the remaining amount of culture solution inside the glass tube. In combination with observing the growth conditions of the plants inside the culture dishes in different groups, the influence of the non-biological external environment on the growth of the plants can be judged more intuitively. At the same time, the device can simultaneously carry out experiments on multiple external factor control variables, thereby shortening the time required for non-biological stress experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of a front cross-sectional structure of a plant culture device for plant abiotic stress experiments proposed by the present invention;

[0025] Figure 2 This is a schematic diagram of the front cross-sectional structure of a first cover plate of a plant culture device for plant abiotic stress experiments proposed by the present invention;

[0026] Figure 3 This is a schematic diagram of the front view of a plant culture device for plant abiotic stress experiments proposed by the present invention;

[0027] Figure 4 A schematic diagram of a top view of a rotating plate of a plant culture chamber for plant abiotic stress experiments proposed by the present invention;

[0028] Figure 5 A plant culture device for plant abiotic stress experiments proposed by the present invention Figure 1 A schematic diagram of the structure enlargement in the middle;

[0029] Figure 6 A plant culture device for plant abiotic stress experiments proposed by the present invention Figure 1 Enlarged schematic diagram of the structure at point B in the middle.

[0030] Legend: 1. Casing; 2. Storage plate; 3. Turntable; 4. Culture dish; 5. Glass tube; 6. Transfer tube; 7. Water pipe; 8. Cotton block; 9. Through pipe; 10. Water-absorbing cotton strip; 11. Heating rod; 12. Ultraviolet lamp; 13. Humidifier; 14. Fan; 15. Gear ring; 16. Motor; 17. Gear; 18. First cover plate; 19. Carbon dioxide generator; 20. Second cover plate; 21. Draft fan; 22. Gas collecting pipe; 23. One-way valve; 24. Bracket; 25. Culture bottle; 26. Rubber stopper; 27. Through hole; 28. Slide groove; 29. ​​Slide plate; 30. Transparent cover plate; 31. Lighting lamp; 32. Round hole. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] See also Figure 1-6 The present invention provides a technical solution: a plant culture device for abiotic stress experiments of plants, comprising a housing 1, a group of storage plates 2 fixedly connected to the inner wall of the housing 1, a group of transparent cover plates 30 arranged on the front of the housing 1, and a rotating plate 3 rotatably connected to the upper surface of each storage plate 2, the transparent cover plate 30 is hinged to the housing 1 and the front of the storage plate 2, and the outer surface of the transparent cover plate 30 is snap-fitted to the inner wall of the housing 1, a bracket 24 is fixedly connected to the upper part of the left side of the second cover plate 20, and a culture bottle 25 is snap-fitted to the upper surface of the bracket 24, and the culture bottle 25 is used as a reference object to facilitate observation of the effects of humidity, temperature, ultraviolet rays and wind speed on the plants inside the housing 1 as a control For reference, the inner top wall of the casing 1 and the bottom surface of the storage plate 2 are fixedly connected with a lighting lamp 31, which provides lighting for the plants inside the casing 1, and the outer surface of the rotating plate 3 is fixedly connected with a gear ring 15. A motor 16 is embedded on the right side of the upper surface of the storage plate 2, and the output end of the motor 16 is fixedly connected with a gear 17 meshing with the gear ring 15. The gear 17 and the gear ring 15 are not in contact with the inner wall of the casing 1 to prevent unnecessary friction when the motor 16 drives the rotating plate 3 to rotate. The motor 16 drives the gear 17 to rotate, so that the gear ring 15 meshing with the gear 17 drives the rotating plate 3 to rotate, which is convenient for observing the growth conditions of plants at different positions in each culture dish 4.

[0033] The right side of the casing 1 is fixedly connected to a first cover plate 18, and the middle part of the right side of the first cover plate 18 is fixedly connected to a carbon dioxide generator 19. There are many types of carbon dioxide generators 19, and their working principles and required energy are also different. Some generators, such as simple devices that produce carbon dioxide through chemical reactions, may not require an external power source. These devices usually rely on the chemical reaction produced when two or more chemical substances are mixed to release carbon dioxide. However, there are also some more complex carbon dioxide generators 19, such as those large equipment used in industry or laboratories, which may require an external power source to drive their internal mechanical components, such as compressors, pumps or heating elements, to ensure the stable generation and output of carbon dioxide. The carbon dioxide generator 19 of the present application uses a chemical reaction to produce carbon dioxide, and a circular hole 32 is opened on the left side of the first cover plate 18. The first cover plate 18 and the carbon dioxide generator 19 are arranged to provide carbon dioxide for the plants inside the casing 1. The left side of the casing 1 is fixedly connected to a second cover plate 20 The middle part of the left side of the second cover plate 20 is fixedly connected with an induced draft fan 21, and the air outlet end of the induced draft fan 21 is fixedly connected with an air collecting pipe 22. The operation of the induced draft fan 21 ensures the fluidity of the air inside the casing 1. The left end of the air collecting pipe 22 is connected to the external gas receiving device. The air collecting pipe 22 discharges and collects the inhaled air. A group of one-way valves 23 are provided at the fixed connection between the second cover plate 20 and the casing 1. The air inlet end of the one-way valve 23 is connected to the interior of the casing 1, and the air outlet end of the one-way valve 23 is connected to the interior of the second cover plate 20. The one-way valve 23 is provided at the fixed connection between the second cover plate 20 and the casing 1. The arrangement ensures the flow direction of the air inside the casing 1, and prevents the air with different humidity or temperature inside the second cover plate 20 from affecting the plants on the upper surface of the storage plate 2 inside the casing 1. A group of culture dishes 4 are fixedly connected to the upper surface of the rotating plate 3. The setting of multiple culture dishes 4 increases the experimental group for reference and reduces misjudgment. A group of glass tubes 5 are fixedly connected to the left side of the casing 1. An appropriate amount of culture solution of the same height is added to the inside of each glass tube 5. Plants to be cultured are placed in the inside of each culture dish 4. As the growth conditions of the plants differ, the culture solutions absorbed by them are also different. The influence of external factors on the plants can be judged by observing the remaining amount of culture solution inside the glass tube 5. That is, if the remaining amount of culture solution inside the glass tube 5 is small, it means that the plant growth is relatively good, and the external factors have a small influence or a positive influence on the plant growth. On the contrary, when the remaining amount of culture solution inside the glass tube 5 is large, it means that the plant grows slowly, and the external factors have a large influence or a negative influence on the plant growth. By observing the growth conditions of the plants inside multiple culture dishes 4 in different groups, the influence of non-biological external factors on plant growth can be more intuitively judged.

[0034] There are many plant species commonly used in plant abiotic stress experiments. Millet is a pioneer plant that is resistant to stress and is also a smart food crop in the future. In research, millet is often used to explore its response mechanism to alkaline stress, drought stress, etc. Arabidopsis is a commonly used model plant, and its genome has been completely sequenced. In plant abiotic stress research, Arabidopsis is often used to explore stress signal transduction pathways, gene expression regulation and other mechanisms; corn is one of the important food crops in the world. In plant abiotic stress research, corn is often used to explore its adaptation mechanism to stress such as drought and salinity. Through genetic engineering and other means, new corn varieties with stress resistance can be cultivated; rice is one of the main food crops in Asia. In plant abiotic stress research, rice is often used to explore its adaptation mechanism to flooding, salinity and other stresses. For example, some studies have explored the role of aerenchyma formation in coping with waterlogging environments through rice. In summary, the plant species used in plant abiotic stress experiments are diverse, aiming to explore the response mechanisms and adaptation strategies of different plants to abiotic stress. By selecting appropriate plant species and experimental designs, we can gain a deeper understanding of the survival strategies and adaptation mechanisms of plants in adversity, and provide theoretical and technical support for the cultivation of new stress-resistant plant varieties. Adjusting the pH of plant culture requires comprehensive consideration of the soil, nutrient solution, and plant needs. Ferrous sulfate is a common soil pH regulator that can lower the pH value of the soil while supplementing the iron required by plants; sodium hydroxide or potassium hydroxide, in the acidic water areas in the south, the pH of the soil can be adjusted by adding sodium hydroxide or potassium hydroxide. When using, dilute it with water in a certain proportion and then irrigate the soil. By selecting suitable soil, using regulators, homemade solutions, and regular testing, you can ensure that plants grow healthily in a suitable pH environment.

[0035] A rubber plug 26 is clamped on the upper surface of the glass tube 5. A group of through holes 27 connected to the glass tube 5 are opened on the upper surface of the rubber plug 26. A slide plate 29 is slidably connected to the inner wall of the slide groove 28 opened on the left side of the rubber plug 26. The slide plate 29 can be pulled to adjust the speed of the culture solution in the glass tube 5 flowing downward to prevent the culture solution in the glass tube 5 from flowing too fast and causing the culture solution in the culture dish 4 to overflow. The transfer tube 6 embedded on the upper surface of the rotating plate 3 is connected to the interior of the glass tube 5 through the water pipe 7, that is, the left end of the water pipe 7 is connected to the glass tube 5, and the right end of the water pipe 7 is fixedly connected to the inner wall of the transfer tube 6. A group of absorbent cotton strips 10 are fixedly connected to the upper surface of the cotton block 8 rotatably connected to the inner wall of the transfer tube 6. Each absorbent cotton strip 10 extends to the interior of a culture dish 4 respectively to provide nutrients required for the growth of plants cultured in the culture dish 4. A group of through pipes are fixedly connected to the upper surface of the cotton block 8. 9, the through tube 9 is rotatably connected to the inner wall of the transfer tube 6, the upper end of the through tube 9 is fixedly connected to the bottom surface of the culture dish 4, the absorbent cotton strip 10 passes through the through tube 9 and extends to the inside of the culture dish 4, the through tube 9 protects the absorbent cotton strip 10 and slows down the evaporation of the absorbent cotton strip 10 during the diversion process of the culture solution, the middle of the upper surface of the three rotating plates 3 are respectively fixedly connected with a heating rod 11, an ultraviolet lamp 12 and a humidifier 13, and the lower part of the right side of the casing 1 is fixedly connected with a fan 14, the coordinated arrangement of the heating rod 11, the ultraviolet lamp 12, the humidifier 13 and the fan 14 enables the device to simultaneously carry out the influence of temperature, humidity, ultraviolet rays and wind speed on the plants inside the culture dish 4, ignoring the influence of the heating rod 11 on the evaporation of the culture solution in the culture dish 4 and the natural evaporation phenomenon of the culture solution in the device culture dish, the device can simultaneously carry out experiments of multiple external factors controlling variables, shortening the time required for abiotic stress experiments.

[0036] Working principle: When in use, the heating rod 11, the ultraviolet lamp 12, the humidifier 13, the fan 14, the motor 16 and the induced draft fan 21 are connected to the power supply, and an appropriate amount of culture solution of the same height is added to the inside of each glass tube 5. The plants to be cultured are placed in the inside of each culture dish 4. The culture solution absorbed by the plants varies with the growth conditions of the plants. The influence of external factors on plant growth can be judged by observing the remaining amount of culture solution in the glass tube 5. That is, if the remaining amount of culture solution in the glass tube 5 is small, it means that the plant growth is relatively good, and the external factors have little effect on the plant growth or have a positive effect. On the contrary, when the remaining amount of culture solution in the glass tube 5 is large, it means that the plant grows slowly, and the external factors have a greater impact on the plant growth or have a negative impact. By observing the growth conditions of plants in multiple culture dishes 4 in different groups, the influence of non-biological external factors on plant growth can be judged more intuitively. At the same time, the device can simultaneously carry out experiments on multiple external factor control variables to shorten the time required for non-biological stress experiments.

[0037] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A plant incubator for abiotic stress experiments on plants, comprising a housing (1), a set of storage plates (2) fixedly connected to the inner wall of the housing (1), a set of transparent cover plates (30) arranged on the front of the housing (1), and a rotating plate (3) rotatably connected to the upper surface of each storage plate (2), characterized in that: A group of culture dishes (4) are fixedly connected to the upper surface of the rotating plate (3); a group of glass tubes (5) are fixedly connected to the left side of the casing (1); a transfer tube (6) embedded in the upper surface of the rotating plate (3) is connected to the inside of the glass tube (5) through a water pipe (7); a group of absorbent cotton strips (10) are fixedly connected to the upper surface of a cotton block (8) rotatably connected to the inner wall of the transfer tube (6); each absorbent cotton strip (10) extends to the inside of a culture dish (4); a heating rod (11), an ultraviolet lamp (12) and a humidifier (13) are fixedly connected to the middle of the upper surfaces of the three rotating plates (3); and a fan (14) is fixedly connected to the lower part of the right side of the casing (1).

2. A plant incubator for plant abiotic stress experiments according to claim 1, characterized in that: A group of through tubes (9) are fixedly connected to the upper surface of the cotton block (8), the upper ends of the through tubes (9) are fixedly connected to the bottom surface of the culture dish (4), and the absorbent cotton strips (10) pass through the through tubes (9) and extend to the interior of the culture dish (4).

3. A plant incubator for plant abiotic stress experiments according to claim 1, characterized in that: The outer surface of the rotating plate (3) is fixedly connected with a gear ring (15), the right side of the upper surface of the storage plate (2) is inlaid with a motor (16), and the output end of the motor (16) is fixedly connected with a gear (17) meshing with the gear ring (15).

4. A plant incubator for plant abiotic stress experiments according to claim 1, characterized in that: The right side of the housing (1) is fixedly connected to a first cover plate (18), the middle of the right side of the first cover plate (18) is fixedly connected to a carbon dioxide generator (19), and a circular hole (32) is provided on the left side of the first cover plate (18).

5. A plant incubator for plant abiotic stress experiments according to claim 1, characterized in that: The left side of the casing (1) is fixedly connected to a second cover plate (20), the middle part of the left side of the second cover plate (20) is fixedly connected to an induced draft fan (21), and the air outlet end of the induced draft fan (21) is fixedly connected to an air collecting pipe (22).

6. A plant incubator for plant abiotic stress experiments according to claim 5, characterized in that: A set of one-way valves (23) are provided at the fixed connection point between the second cover plate (20) and the casing (1); the air inlet end of the one-way valve (23) is connected to the interior of the casing (1), and the air outlet end of the one-way valve (23) is connected to the interior of the second cover plate (20).

7. A plant incubator for plant abiotic stress experiments according to claim 6, characterized in that: A bracket (24) is fixedly connected to the upper portion of the left side of the second cover plate (20), a culture bottle (25) is clamped on the upper surface of the bracket (24), and an illumination lamp (31) is fixedly connected to the inner top wall of the housing (1) and the bottom surface of the storage plate (2).

8. A plant incubator for plant abiotic stress experiments according to claim 1, characterized in that: A rubber plug (26) is clamped on the upper surface of the glass tube (5), and a group of through holes (27) connected to the glass tube (5) are opened on the upper surface of the rubber plug (26). A slide plate (29) is slidably connected to the inner wall of a sliding groove (28) opened on the left side of the rubber plug (26).

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