Method for judging migration flight state of cnaphalocrocis medinalis by combining sex pheromone and flower fragrance trapping

Abstract

The invention discloses a method for judging the migratory flight state of Cnaphalocrocis medinalis by combining sex pheromone and flower fragrance trapping, and belongs to the technical field of biology.The method comprises the steps that a sex pheromone trapping core is prepared; preparing a floral lure; placing and recording a trap; processing by a sex luring intelligent forecasting system; processing by an intelligent moth-expelling recognition system; and judging whether the rice leaf folders are immigration generations or local breeding generations and emigration generations according to the quantity proportion of sex-induced rice leaf folders and flower-induced rice leaf folders. The invention provides a method for judging the migratory flight state of field rice leaf folders, calculating the moth quantity of the field rice leaf folders and predicting the occurrence time of next-generation eggs and one-year-old larvae by combining sex pheromones and flower fragrance trapping, so that the method can replace the current methods of ovary dissection, moth expelling in the early morning in the field and field investigation by grass-roots plant protectors. The working efficiency of basic plant protection technicians is greatly improved, and the accuracy and scientificity of prediction and forecasting are greatly improved.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and more particularly to a method for judging the migration state of rice leaf folders and predicting the number of field populations by combining sex pheromones with flower scent trapping. Background Art

[0002] The rice leaf folder, Cnaphalocrocis medinalis, is an important migratory pest of rice. It causes leaf damage and significantly affects rice yield when it occurs in large numbers. The rice leaf folder is not very phototactic to the monitoring lamp. The method of catching moths in the early morning is generally adopted in various places for monitoring, but this method has a large workload and large errors. In addition, the ovary needs to be dissected to determine its migration status (Zhang Xiaoxi et al., 1981). The development level of the ovary and the proportion of mating are obtained based on the ovarian dissection. The migration status (three migration states: immigration generation, local reproduction generation and migration generation) is determined by the percentage of female moths with ovary development level I, mating rate, and population growth and decline. This is a difficult problem for grassroots plant protection stations. There are not many professionals at the grassroots level who can operate in this way. Therefore, how to simply and accurately monitor the population dynamics of rice leaf folders in the field has always been a difficult problem that urgently needs to be solved in production. The target of sex pheromone trapping is the sexually mature male moth that is seeking mates. The sex pheromones released by female adults of the rice leaffolder are Z11-18:Ald, Z13-18:Ald, Z11-18:OH and Z13-18:OH (11:100:24:36) (Kawazu et al., 2002; Wu et al., 2013). However, in actual field trapping, the instability of sex pheromones is a significant weakness of sex pheromone detection. Migratory insects such as the rice leaffolder, the oriental armyworm Mythimna separata, and the meadow moth Loxostegesticticalis are generally believed to have immature ovaries after eclosion, partially mate during migration, lay eggs after landing, and then need to supplement nutrition. Therefore, the strong smell of flowers attracts the migrating generation of rice leaffolders (Guo Qianshuang et al., 2022). There are many reports on the use of floral scent to attract Lepidoptera and Diptera (Meagher, 2002; Meagher & Landolt, 2010). However, because the active compounds based on floral scent do not have the specificity of sex pheromones, the attraction response to non-target pests, pollinating beneficial insects, and even natural enemies is also very significant and strong (Meagher & Mitchell, 1999; Rohrig et al., 2008). Based on these negative effects in the ecosystem, floral scent trapping is gradually being used for the purpose of biodiversity surveys (Landolt et al., 2007). The anatomical characteristics of the ovaries of different insect sources are shown in the table below:

[0003]

[0004] N 0 = weighted average number of remaining larvae and pupae of the previous generation; N = actual number of moths driven away in the previous generation.

[0005] The olfactory response of some long-lived insects to sex pheromones is closely related to their physiological state (Evened & Gries, 2008). Age is an important factor. For example, the newly emerged male cutworm Agrotis ipsilon does not respond to sex pheromones, and its olfactory response ability increases with age and sexual maturity (Gemeno & Haynes, 2000). The same is true for the rice leaf roller. Mating is another important factor (Reyes et al., 2015). Because of the differences in the migration state, larval source and habitat of the rice leaf roller, the physiological state of the adult nutrition, mating and ovarian development is relatively complex, and the adult can mate multiple times, and migration and mating can be carried out at the same time. Therefore, it is common for rice leaf rollers in the same place at the same time to have different lifespans, ages, sexual development levels, mating states, etc. Obviously, the migration, seasonality, ovary and testis development of rice leaf rollers are closely related to the sexual attraction of rice leaf rollers (Feng Bo et al., 2017). The basic migrating rice leaf folder may have mated during the migration process. After landing, there is a period of behavioral inactivity between mating intervals. After that, it first responds to sex pheromones, and then responds to the scent of flowers in the later stage of egg-laying. The female moths induced by flower scent have a relatively high ovarian level, and the proportion of ovarian level V and egg-laying is much higher than that of female moths caught in nets. The local breeding type rice leaf folder has a strong response to sex pheromones, but the effect of flower scent trapping is poor. For the local breeding type population, the number of mating times of female moths trapped by flower scent is significantly higher than that of those caught in the field. Most of the migrating types have a low response to trapping with sex pheromones and floral scents. The size of the testes of male moths is the largest in field nets, and the size of those induced by sex pheromones is larger than that of those induced by floral scents (Guo Qianshuang et al., 2022). At present, the methods for predicting and forecasting rice leaf folders in production are: 1) the moth-chasing method to estimate the number of moths in the field; 2) dissecting the ovaries of female moths, observing the ovarian level and mating status, and judging the migration status. These two methods are difficult to implement at the grassroots level, and there are fewer and fewer plant protection stations that can do these two tasks.

[0006] Therefore, providing a method for determining the migration status of rice leaf folder by combining sex pheromone and floral scent trapping is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0007] In view of this, the present invention provides a method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping.

[0008] In order to achieve the above object, the present invention adopts the following technical solution:

[0009] A method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping comprises the following steps:

[0010] 1) Sex pheromone lure: Place the sex pheromone of rice leaf folder in the capillary liquid filling structure lure to form the sex pheromone lure;

[0011] 2) Flower scent attractant core: Add the flower scent attractant core into a black ventilated modified polyethylene plastic bag;

[0012] 3) Traps: Use a new type of moth trap with an inverted funnel. Install a sex pheromone lure or a floral scent lure in each trap. Collect the rice leaf rollers in the trap every morning and count and record.

[0013] 4) Sex-induced intelligent monitoring and reporting system: Sex pheromones are used to trap rice leaf folders in the field, and infrared sensors are used to sense and record the number of trapped rice leaf folders in real time; the data of trapped rice leaf folders are transmitted to the server through a wireless network, and the occurrence time of the next generation of eggs and first-instar larvae is predicted in combination with the future temperature data of the Meteorological Bureau;

[0014] 5) Intelligent moth-chasing and identification system: fully automatically collects and analyzes the number of rice leaf rollers in the field, the rotating moth-chasing device automatically chases away the moths, and uses industrial cameras to record pest activity videos in real time. After uploading to the server, accurate counting and analysis are performed based on image recognition algorithms;

[0015] 6) The ratio of the number of rice leaf folders attracted by sex and fragrance can be used to determine whether it is the immigrant generation, local breeding generation, or the emigrant generation.

[0016] Furthermore, the sex pheromone in step 1) is composed of cis-13-octadecenal, cis-11-octadecenal, cis-13-octadecenol and cis-11-octadecenol in a mass ratio of 50-1000:5-100:12-240:9-180;

[0017] The capillary liquid filling structure wick is made of polyvinyl chloride or polyethylene.

[0018] Furthermore, the floral scent attractant in step 2) is a plant-derived non-sex pheromone compound;

[0019] Each floral scent attractant contains 20g of plant-derived non-sex pheromone compounds.

[0020] Furthermore, the plant-derived non-sex pheromone compound in step 2) is a mixture of any one or more of benzaldehyde, phenylacetaldehyde, phenylethanol, methyl salicylate and linalool.

[0021] Furthermore, in the technical solution of the present invention, the black breathable modified polyethylene plastic bag can be made of any breathable polyethylene material prepared by existing technology, or by directly purchasing a breathable polyethylene material. It is not limited to a specific modified polyethylene material, and any breathable polyethylene material can be used.

[0022] Furthermore, the black ventilated modified polyethylene plastic bag in step 2) has a specification of 86 mm in length, 72 mm in width and 0.15 mm in thickness.

[0023] Furthermore, in step 3), the traps for installing the sex pheromone lure core are 0.8-1.0 m above the ground, and there are 6 of them;

[0024] The traps with floral scent lures are 1-1.2m above the ground and there are 6 of them;

[0025] The sex pheromone attractant core and the floral scent attractant core are replaced once every two months.

[0026] Further, the intelligent moth-chasing identification system in step 5) includes a rotating moth-chasing device;

[0027] The rotating moth-chasing device comprises a base, a support rod, a motor, a metal pipe, a camera, an electrical box and a solar panel;

[0028] Wherein, the bottom end of the support rod is fixedly connected to the base.

[0029] The motor is fixed at the middle position of the support rod, the metal tube is fixedly connected to the motor, and the metal tube is perpendicular to the support rod;

[0030] The camera is fixedly connected to the support rod, and the camera is located directly above the motor;

[0031] The electrical box is fixedly connected to the support rod, and the electrical box and the camera are symmetrical with the support rod as the center;

[0032] The solar panel is fixedly connected to the support rod via a solar panel bracket;

[0033] The motor, the camera and the solar panel are all electrically connected to the electrical box.

[0034] Further, the working mode of the intelligent moth-chasing identification system in step 5) is as follows:

[0035] When the long pole finishes rotating to drive away the moth, the video recording ends, and the system transmits the video data to the server via the wireless network;

[0036] On the server side, after reading the video frame, first perform histogram equalization on its brightness channel to complete preprocessing; then, use the large model SAM to segment the image, obtain the mask of each image part, calculate the area of ​​each mask through edge detection, remove the mask that does not contain rice leaf folder, and the remaining masks are the rice leaf folder targets. Fit the remaining masks with an external rectangle to obtain the detection frame and count them. Finally, draw the detection results on the original video, and count the number of rice leaf folders that were driven away during this moth-chasing operation.

[0037] Further, in step 6), the method of interrupting whether it is the immigration generation or the local reproduction generation and the migration generation is:

[0038] When the number of sexually attracted fish is significantly lower than half of that of fish trapped by scent of flowers, this period is the immigration generation; when the number of sexually attracted fish is significantly higher than half of that of fish trapped by scent of flowers, this period is the local breeding generation.

[0039] The beneficial effects of the present invention are:

[0040] Technical principle: In every rice-growing area in my country, the first population of rice leaf rollers migrated from other places (immigrant generation), followed by local reproduction generation after mating and reproduction, and then the outmigrant generation (from September to October when the rice is ripe). Figure 1 ). Since the sexual attractants are young male moths that need courtship, or even unmated, the female moths are also in a corresponding state, while the floral scent attractants are female moths that have mated and laid eggs at least once, and male moths that have mated once. Therefore, the incoming generation generally lands multiple times during the migration process, and many of them have mature ovaries and have mated during this process, and lay eggs after landing. Therefore, the incoming generation has a high ovary level, and those that have already mated have a mating dormant period. These female and male moths react strongly to floral scent attractants, but because they have already mated, they react less to sexual attractants. Therefore, the sexual attractant of the incoming generation is much lower than that of floral scent attractants. The local breeding generation is different and does not need to migrate. Therefore, the number of sexually attracted male moths is greater than that of floral scent attractants. The adults of the outgoing generation need to migrate out, and both males and females are not mature, so they cannot be attracted by sexual attractants or floral scent attractants.

[0041] It can be seen from the above technical solutions that, compared with the prior art, the present invention discloses a method for combining sex pheromone and flower scent trapping to judge the migration status of rice leaf folders in the field, estimate the amount of rice leaf folders in the field, and predict the occurrence time of the next generation of eggs and first-instar larvae, thereby replacing the current methods of ovarian dissection, field early morning moth driving and field investigation by grassroots plant protection workers, greatly reducing the work efficiency of grassroots plant protection technicians and the accuracy and scientificity of prediction and forecasting. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 This is a comparison chart of the sex lure and the floral scent lure of the rice leaf folder;

[0043] Figure 2 A comparison of sex-attractant and floral-scented trapping for the incoming rice leaf folder population.

[0044] Figure 3 Comparison of sex- and scent-based traps for local populations of rice leaf folder;

[0045] Figure 4 This is the relationship between the amount of moths attracted by sex pheromone and the amount of moths migrating into the field;

[0046] Figure 5 This is the relationship between the amount of moths attracted by the flower scent and the amount of moths migrating into the field;

[0047] Figure 6 This is a graph showing the relationship between the amount of moths attracted by sex pheromones and floral scents and the amount of moths per mu that reproduce locally in the field;

[0048] Figure 7 This is the structural diagram of the double funnel trap in the sex-attracting intelligent detection and reporting system;

[0049] Figure 8 This is the structural diagram of the camera of the insect collector on the upper part of the double funnel trap in the sex-attracting intelligent monitoring and reporting system;

[0050] Fig. 9 This is the overall structure diagram of the intelligent moth-chasing and identification system;

[0051] Fig.10 This is a video recognition flow chart for the automatic moth-catching and counting system in the field;

[0052] Fig.11 This is a photo illustration of the video of automatic moth-chasing in the field. DETAILED DESCRIPTION

[0053] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0054] Example 1

[0055] A method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping comprises the following steps:

[0056] 1) Sex pheromone attractant: Place the sex pheromone of rice leaf folder in the capillary liquid structure attractant to form the sex pheromone attractant; the sex pheromone consists of 50-1000 μg of cis-13-octadecenal, 5-100 μg of cis-11-octadecenal, 12-240 μg of cis-13-octadecenol and 9-180 μg of cis-11-octadecenol; the capillary liquid structure attractant is polyvinyl chloride or polyethylene.

[0057] 2) Flower scent attractant core: add the flower scent attractant core into a black ventilated modified polyethylene plastic bag; the plant-derived non-sex pheromone compound in the flower scent attractant core is one of benzaldehyde, phenylacetaldehyde, phenylethanol, methyl salicylate and linalool, and each flower scent attractant core contains 20g of the plant-derived non-sex pheromone compound; the specifications of the black ventilated modified polyethylene plastic bag are 86mm in length, 72mm in width and 0.15mm in thickness;

[0058] 3) Traps: Use a new type of inverted funnel moth trap, each of which is equipped with a sex pheromone lure or a floral scent lure. Collect the rice leaf rollers in the trap every morning and count and record; the trap equipped with the sex pheromone lure is 0.8-1.0m above the ground, and there are 6 traps; the trap equipped with the floral scent lure is 1-1.2m above the ground, and there are 6 traps; the sex pheromone lure and the floral scent lure are replaced once every 2 months.

[0059] 4) Sex-induced intelligent monitoring and reporting system: Sex pheromones are used to trap rice leaf folders in the field, and infrared sensors are used to sense and record the number of trapped rice leaf folders in real time; the data of trapped rice leaf folders are transmitted to the server through a wireless network, and the occurrence time of the next generation of eggs and first-instar larvae is predicted in combination with the future temperature data of the Meteorological Bureau;

[0060] 5) Intelligent moth-chasing and identification system: fully automatically collects and analyzes the number of rice leaf rollers in the field, the rotating moth-chasing device automatically chases away the moths, and uses industrial cameras to record pest activity videos in real time. After uploading to the server, accurate counting and analysis are performed based on image recognition algorithms;

[0061] 6) The ratio of the number of rice leaf folders attracted by sex and scent was used to determine whether it was the incoming generation, the local reproductive generation, or the outgoing generation; if the number of sex-lured insects was significantly lower than 1 / 2 of those attracted by scent, this period was the incoming generation; if the number of sex-lured insects was significantly higher than 1 / 2 of those attracted by scent, this period was the local reproductive generation (outgoing generation).

[0062] In some embodiments, the sex-induced intelligent monitoring system is used to automatically monitor the number of rice leaf rollers and predict the number of eggs and larvae.

[0063] The core of the system is an inverted reverse double funnel trap ( Figure 7, Ningbo Newcom Biotechnology Co., Ltd.'s SPT-R-02S trap, which is designed based on the behavioral characteristics of the rice leaf roller's multi-sensory channels to design the color, reflectivity, shape and structure of the trap to maximize the lure of rice leaf rollers. The upper and lower insect collectors of the trapping system each have a three-layer 4-pair infrared counting device, which automatically counts the insects entering the insect collector by matching the target insect's surface area, movement speed and trajectory through a multi-channel sensing algorithm. The design of the multi-layer infrared counting device can avoid repeated counting and missing counting, and can also cope with the high concurrency during the peak period of rice leaf rollers to ensure the accuracy of the data.

[0064] There is a camera on the top of the insect collector ( Figure 8 , Ningbo Newcom Biotechnology Co., Ltd.'s SPT-R-02S trap), the camera takes pictures at regular intervals every day and transmits the image data to the server. The main function of the camera is to use the image data for automatic calibration when the number of traps increases suddenly, avoiding the error caused by relying solely on infrared counting. At the same time, when there are too many pests in the trap, the system will issue a timely cleaning reminder to ensure the continuous and efficient operation of the system.

[0065] The pest data obtained by the trapping system is transmitted to the server via wireless network. On the server side, the system not only counts the current number of pests, but also combines these data with the temperature data of the next 10 days obtained from the Meteorological Bureau. Based on the functional relationship between temperature and the development rate of rice leaf rollers, the system automatically predicts the eggs and larvae of rice leaf rollers in the future and automatically generates a trend chart.

[0066] In some embodiments, the intelligent moth-repelling identification system includes a rotating moth-repelling device;

[0067] The rotating moth-chasing device comprises a base, a support rod, a motor, a metal pipe, a camera, an electrical box and a solar panel;

[0068] Wherein, the bottom end of the support rod is fixedly connected to the base.

[0069] The motor is fixed at the middle position of the support rod, the metal tube is fixedly connected to the motor, and the metal tube is perpendicular to the support rod;

[0070] The camera is fixedly connected to the support rod, and the camera is located directly above the motor;

[0071] The electrical box is fixedly connected to the support rod, and the electrical box and the camera are symmetrical with the support rod as the center;

[0072] The solar panel is fixedly connected to the support rod via a solar panel bracket;

[0073] The motor, the camera and the solar panel are all electrically connected to the electrical box.

[0074] In other embodiments, the intelligent moth-repelling identification system works as follows:

[0075] The rotating moth-chasing device automatically starts at a preset time, and the motor drives the long pole to rotate, simulating the traditional manual moth-chasing method. The rotation range and speed of the long pole are precisely designed to effectively cover a large area of ​​the field. When the long pole starts to rotate, the industrial camera fixed on the top of the long pole automatically starts to record the entire moth-chasing process in real time, ensuring that the movement trajectory and group situation of the rice leaf roller are fully captured.

[0076] When the long pole is rotated to drive away the moths, a short video delay mechanism is set to ensure that all dynamic behaviors of the rice leaf roller are fully recorded. When the video recording is finished, the system transmits the video data to the server through the wireless network.

[0077] On the server side, after reading the video frame, first perform histogram equalization on its brightness channel to complete preprocessing; then, use the large model SAM to perform image segmentation, obtain the mask of each image part, and calculate the area of ​​each mask through edge detection. Since the area of ​​rice leaf folder in the video frame is small, the mask without rice leaf folder can be eliminated, and the remaining mask is the target of rice leaf folder. The remaining mask is fitted with an external rectangle to obtain the detection frame and count it. Finally, the detection result is drawn on the original video, and the number of rice leaf folders in this moth expulsion is counted.

[0078] Example 2

[0079] According to the method of Example 1, the migration status (immigrant generation, local reproduction generation and emigrant generation) of rice leaf folder in the field is determined.

[0080] Every morning, the rice leaf rollers in the trap were collected and counted. The ratio of the number of sexual lures and the number of floral scent lures was used to determine whether it was the immigrant generation, the local breeding generation, or the out-migration generation. The number of sexual lures was significantly lower than 1 / 2 of the floral scent lures, and this period was the immigrant generation ( Figure 2 The number of sexual traps was significantly higher than that of scent traps by half, and this period was the time of local reproduction ( Figure 3 ).

[0081] Example 3

[0082] According to the method of Example 1, the number of adult insects in the field was estimated using the data of sexual attraction and floral fragrance attraction.

[0083] 1) Calculation method of the number of moths in the field during the migration

[0084] The average number of moths caught per day by the influx of pheromone was 12.6±6.7, and the number of moths caught by sex pheromone was only 5.1±2.8. The Pearson correlation coefficient was calculated, i.e., r=0.768, P=0.004, indicating that there was a significant correlation between the number of moths caught by sex pheromone and the number of moths caught in the field. The fitting curve was y=-0.4058x 2 +23.759x+84.478. There was no correlation between flower scent trapping and the number of moths caught in the field (r=0.441, P=0.151).

[0085] 2) Calculation method of the number of moths in the local breeding field

[0086] The average number of moths trapped per day by flower scent was 2.3±0.5, and that by sex pheromone was 17.5±5.2. The Pearson correlation coefficient was calculated, i.e., r=0.582, P=0.018, indicating that there was a significant correlation between sex pheromone trapping and the number of moths driven away in the field. The fitting curve was y=-0.0438x 2 +5.5435x+99.793. There was no correlation between flower scent trapping and the number of moths caught in the field (r=0.101, P=0.708).

[0087] Example 4 Predicting the hatching time of eggs using sex inducement according to the method of Example 1

[0088] According to the time of trapping rice leaf folder with sex pheromones in the field, the temperature data of the next 10 days from the Meteorological Bureau was downloaded and imported. Using the functional relationship between temperature and development rate obtained in the experiment, the time range of the egg stage and larval development to the first instar of rice leaf folder in the field could be calculated.

[0089] Time interval from mating to egg laying (d) y＝1.1 Relationship between egg development rate and temperature (d) <![CDATA[y=-0.0008x 2 +0.0627x-0.8513]]> <![CDATA[R 2 =0.998]]> Relationship between larval development rate and temperature (d) y=0.0055x-0.094 <![CDATA[R 2 =0.934]]>

[0090] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping, characterized in that: The following steps are involved: 1) Sex pheromone lure: Place the sex pheromone of rice leaf folder in the capillary liquid filling structure lure to form the sex pheromone lure; 2) Flower scent attractant core: Add the flower scent attractant core into a black breathable modified polyethylene plastic bag; 3) Traps: Use a new type of moth trap with an inverted funnel. Install a sex pheromone lure or a floral scent lure in each trap. Collect the rice leaf rollers in the trap every morning and count and record. 4) Sex-induced intelligent monitoring and reporting system: Sex pheromones are used to trap rice leaf folders in the field, and infrared sensors are used to sense and record the number of trapped rice leaf folders in real time; the data of trapped rice leaf folders are transmitted to the server through a wireless network, and the occurrence time of the next generation of eggs and first-instar larvae is predicted in combination with the future temperature data of the Meteorological Bureau; 5) Intelligent moth-chasing and identification system: fully automatically collects and analyzes the number of rice leaf rollers in the field, the rotating moth-chasing device automatically chases away the moths, and uses industrial cameras to record pest activity videos in real time. After uploading to the server, accurate counting and analysis are performed based on image recognition algorithms; 6) The ratio of the number of rice leaf folders attracted by sex and fragrance can be used to determine whether it is the immigrant generation, local breeding generation, or the emigrant generation.

2. The method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping according to claim 1, characterized in that: The sex pheromone in step 1) is composed of cis-13-octadecenal, cis-11-octadecenal, cis-13-octadecenol and cis-11-octadecenol in a mass ratio of 50-1000:5-100:12-240:9-180; The capillary liquid filling structure wick is made of polyvinyl chloride or polyethylene.

3. The method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping according to claim 1, characterized in that: The floral scent attractant in step 2) is a plant-derived non-sex pheromone compound; Each floral scent attractant contains 20g of plant-derived non-sex pheromone compounds.

4. The method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping according to claim 3, characterized in that: The plant-derived non-sex pheromone compound in step 2) is a mixture of any one or more of benzaldehyde, phenylacetaldehyde, phenylethanol, methyl salicylate and linalool.

5. The method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping according to claim 1, characterized in that: The black ventilated modified polyethylene plastic bag in step 2) has a length of 86 mm, a width of 72 mm, and a thickness of 0.15 mm.

6. The method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping according to claim 1, characterized in that: In step 3), the traps with sex pheromone lure cores are installed 0.8-1.0m above the ground, and there are 6 of them; The traps with floral scent lures are 1-1.2m above the ground and there are 6 of them; The sex pheromone attractant core and the floral scent attractant core are replaced once every two months.

7. The method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping according to claim 1, characterized in that: The intelligent moth-chasing identification system in step 5) includes a rotating moth-chasing device; The rotating moth-chasing device comprises a base, a support rod, a motor, a metal pipe, a camera, an electrical box and a solar panel; Wherein, the bottom end of the support rod is fixedly connected to the base. The motor is fixed at the middle position of the support rod, the metal tube is fixedly connected to the motor, and the metal tube is perpendicular to the support rod; The camera is fixedly connected to the support rod, and the camera is located directly above the motor; The electrical box is fixedly connected to the support rod, and the electrical box and the camera are symmetrical with the support rod as the center; The solar panel is fixedly connected to the support rod via a solar panel bracket; The motor, the camera and the solar panel are all electrically connected to the electrical box.

8. The method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping according to claim 7, characterized in that: The working mode of the intelligent moth-chasing identification system described in step 5) is as follows: When the long pole finishes rotating to drive away the moth, the video recording ends, and the system transmits the video data to the server via the wireless network; On the server side, after reading the video frame, first perform histogram equalization on its brightness channel to complete preprocessing; Then, the large model SAM is used for image segmentation to obtain the mask of each image part. The area of ​​each mask is calculated through edge detection, and the masks that do not contain rice leaf folders are eliminated. The remaining masks are the targets of rice leaf folders. The circumscribed rectangles are fitted on the remaining masks to obtain the detection frames and count them. Finally, the detection results are drawn on the original video, and the number of rice leaf folders caught in this moth-chasing operation is counted.

9. The method for judging the migration status of rice leaf folder by combining sex pheromone and flower scent trapping according to claim 1, characterized in that: Step 6) The method of interrupting the immigration generation, local reproduction generation and emigration generation is: When the number of sexually attracted fish is significantly lower than half of that of fish trapped by scent of flowers, this period is the immigration generation; when the number of sexually attracted fish is significantly higher than half of that of fish trapped by scent of flowers, this period is the local breeding generation.

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