Sheep colostrum composite powder composition capable of improving immunity and preparation method of sheep colostrum composite powder composition

Abstract

The invention discloses a sheep colostrum composite powder composition capable of improving immunity and a preparation method of the sheep colostrum composite powder composition, and belongs to the technical field of milk composite powder. Comprising the following components in parts by weight: 15 to 20 parts of compound microcapsule probiotics, 200 to 300 parts of sheep colostrum dry powder, 20 to 25 parts of dissolution promoting polypeptide, 0.005 to 0.009 part of vitamin A, 0.003 to 0.006 part of vitamin D, 0.008 to 0.01 part of ascorbic acid, 0.003 to 0.005 part of vitamin K1, 0.006 to 0.009 part of vitamin B2 and 0.002 to 0.004 part of folic acid. According to the preparation method, the technical effects that the dissolution speed is increased, and caking and precipitation are not prone to occurring are achieved in a mode of preparing the dissolution-promoting polypeptide.

Description

Technical Field

[0001] The invention belongs to the technical field of milk compound powders, and specifically refers to a sheep colostrum compound powder composition for improving immunity and a preparation method thereof. Background Art

[0002] Goat colostrum refers to the milk secreted by the ewe in the first week after giving birth. This milk is rich in immunoglobulins, vitamins, minerals and other nutrients. Goat colostrum contains a large amount of immunoglobulins, such as IgG, which can help build the immune system and resist diseases. Goat colostrum is also rich in protein, fat, carbohydrates and a variety of vitamins and minerals, which can provide comprehensive nutritional support. At the same time, the growth factors in goat colostrum help promote growth and development, are easy to digest, and are suitable for the digestive system of newborns; Lactobacillus rhamnosus has a high intestinal adhesion rate and strong colonization ability. It can play an important physiological and health care function such as regulating intestinal flora, preventing and treating diarrhea, and excreting toxins. It can enhance the function of the immune system and help resist infection; Shiitake mushrooms have thick and tender meat, delicious taste, unique aroma, and rich nutrition. It is a food that is both edible and medicinal, with high nutritional, medicinal and health value. Shiitake mushrooms contain a variety of active ingredients, especially shiitake polysaccharides. Shiitake polysaccharides are polar macromolecular compounds, the main active ingredient of shiitake mushrooms, and a host immune enhancer.

[0003] The existing technology currently has the following main problems: probiotics are easily destroyed by gastric juice, goat colostrum dry powder dissolves slowly in water, is easy to agglomerate, and is easy to precipitate. Summary of the invention

[0004] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a sheep colostrum composite powder composition for improving immunity and a preparation method thereof. In order to solve the problems of slow dissolution speed, easy agglomeration and easy precipitation of sheep colostrum dry powder in water, the present invention proposes to improve the stability of sheep colostrum dry powder in water by preparing a dissolution-promoting polypeptide, thereby achieving the technical effects of increased dissolution speed, less prone to agglomeration and less prone to precipitation. At the same time, Lentinan and whey protein are used to compositely wrap probiotics to make the wrapping tighter and reduce the damage of gastric juice to the probiotics.

[0005] In order to achieve the above-mentioned purpose, the technical scheme adopted by the present invention is as follows: the present invention proposes a sheep colostrum composite powder for improving immunity, and the sheep colostrum composite powder for improving immunity comprises the following components in parts by weight: 15-20 parts of composite microcapsule probiotics, 200-300 parts of sheep colostrum dry powder, 20-25 parts of dissolution-promoting polypeptides, 0.005-0.009 parts of vitamin A, 0.003-0.006 parts of vitamin D, 0.008-0.01 parts of ascorbic acid, 0.003-0.005 parts of vitamin K1, 0.006-0.009 parts of vitamin B2, and 0.002-0.004 parts of folic acid; the composite microcapsule probiotics are lentinan-whey protein microcapsules encapsulating Lactobacillus rhamnosus.

[0006] Preferably, the composite microcapsule probiotics comprises the following components in parts by weight: 30-40 parts of shiitake mushrooms, 2.5-3.5 parts of whey protein and 0.3-0.5 parts of Lactobacillus rhamnosus powder.

[0007] Preferably, the dissolution-promoting polypeptide comprises the following components in parts by weight: 25-30 parts of pea protein powder and 10-12 parts of glucose.

[0008] Preferably, the method for preparing the composite microcapsule probiotics specifically comprises the following steps:

[0009] S1, washing, freeze-drying, crushing and passing through a 100-mesh sieve to obtain mushroom powder, adding the mushroom powder to a 60wt% ethanol solution, transferring the solution to an ultrasonic-microwave extractor, performing ultrasonic-microwave extraction, and centrifuging to obtain a supernatant and a precipitate;

[0010] S2, adding the precipitate obtained in S1 into petroleum ether, reflux extraction, drying, adding into deionized water, ultrasonic extraction, centrifugation, taking the supernatant, concentrating, and ethanol precipitation to obtain Lentinan;

[0011] S3. Add Lactobacillus rhamnosus powder to the supernatant obtained in S1 to obtain a bacterial solution, add whey protein to deionized water, add Lentinan to obtain a cross-linked solution, add the bacterial solution to the cross-linked solution, stir at 700-800 rpm for 1-1.5 h, spray dry, and obtain a composite microcapsule probiotic.

[0012] Preferably, in S1, the shiitake mushroom is added to a 60 wt % ethanol solution in an amount of 0.04-0.06 g / mL.

[0013] Preferably, in S1, the ultrasonic microwave extraction has a temperature of 40-50°C, an acoustic power of 50-60W, a microwave power of 600-700W, and a time of 10-12min.

[0014] Preferably, in S2, the amount of the precipitate obtained in S1 added to petroleum ether is 0.1-0.12 g / mL.

[0015] Preferably, in S2, the reflux extraction temperature is 65-70°C and the time is 2-3h.

[0016] Preferably, in S2, the defatted dry powder is added to deionized water in an amount of 0.2-0.4 g / mL.

[0017] Preferably, in S2, the ultrasonic extraction temperature is 50-60°C, the ultrasonic power is 150-160w, and the time is 30-40min.

[0018] Preferably, in S3, the amount of whey protein added to deionized water is 0.05-0.08 g / mL.

[0019] Preferably, the method for preparing the dissolution-promoting polypeptide comprises the following steps:

[0020] (1) adding pea protein powder to deionized water to obtain a 7-10 wt% pea protein solution, adjusting the pH to 8-9 with a 1 mol / L NaOH solution, and adding alkaline protease at a mass fraction of 1.0% of the pea protein solution to obtain a mixed solution;

[0021] (2) The mixed solution obtained in step (1) was hydrolyzed by shaking in a water bath at 50-60° C. for 3-4 hours, the pH value was maintained constant with 1 mol / L NaOH solution, the pH was adjusted to 7.0, and the enzyme was inactivated at 95° C. for 10 minutes to obtain pea polypeptide;

[0022] (3) Add pea polypeptide to deionized water and glucose, mix well, stir at 60-120 rpm in an oil bath at 90-95°C for 4-6 hours, cool and dry to obtain a dissolution-promoting polypeptide.

[0023] The present invention also provides a method for preparing a sheep colostrum composite powder for improving immunity, which specifically comprises the following steps:

[0024] Add composite microcapsule probiotics, goat colostrum dry powder, dissolution-promoting polypeptide, vitamin A, vitamin D, ascorbic acid, vitamin K1, vitamin B2 and folic acid into a milk powder mixing tank and mix them evenly to obtain goat colostrum composite powder for improving immunity.

[0025] The beneficial effects achieved by the present invention are as follows: the present invention uses lentinan and whey protein as coating materials to attack rhamnosus bacteria, thereby improving the gastric juice survival rate of rhamnosus bacteria. Lentinan is a polar macromolecule that can enhance immunity. At the same time, rhamnosus bacteria is beneficial to the stability of intestinal flora and resists infection. Lentinan is compounded with whey protein to improve the cross-linking network of whey protein and enhance its gastric juice resistance, thereby enhancing the protection of probiotics and lentinan alcohol extracts and maintaining their activity; pea egg polypeptide is glycosylated and modified with glucose to enhance the water solubility of pea polypeptide. The hydrophilicity of the glycosylated part is enhanced, which is beneficial to promoting the dissolution speed of sheep colostrum dry powder in water, improving the dissolution rate, and reducing the generation of lumps. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The results of digestion experiments of Examples 1-3 and Comparative Examples 1-2 of the present invention are shown in FIG.

[0027] Figure 2 The results of the enteric coating experiments of Examples 1-3 of the present invention are shown in FIG.

[0028] Figure 3 The results of the precipitation experiments of Examples 1-3 and Comparative Example 3 of the present invention are shown in FIG.

[0029] Figure 4 The results of the dissolution experiments of Examples 1-3 and Comparative Example 3 of the present invention are shown in FIG.

[0030] Figure 5 The results of the dissolution experiments of Examples 1-3 and Comparative Example 3 of the present invention are shown in FIG.

[0031] Figure 6 This is an electron microscope image of the composite microcapsule probiotics in Example 1 of the present invention.

[0032] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0034] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0035] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0036] Example 1

[0037] A sheep colostrum composite powder for improving immunity comprises the following components in parts by weight: 20 parts of composite microcapsule probiotics, 300 parts of sheep colostrum dry powder, 25 parts of dissolution-promoting polypeptides, 0.009 parts of vitamin A, 0.006 parts of vitamin D, 0.01 parts of ascorbic acid, 0.005 parts of vitamin K1, 0.009 parts of vitamin B2, and 0.004 parts of folic acid.

[0038] The composite microcapsule probiotics comprises the following components in parts by weight: 40 parts of shiitake mushrooms, 3.5 parts of whey protein and 0.5 parts of lactobacillus rhamnosus powder.

[0039] The dissolution-promoting polypeptide comprises the following components in parts by weight: 30 parts of pea protein powder and 12 parts of glucose.

[0040] The preparation method of composite microcapsule probiotics specifically comprises the following steps:

[0041] S1. Wash, freeze-dry, crush and sieve the mushrooms through a 100-mesh sieve to obtain mushroom powder. Add 0.06 g / mL of the mushroom powder to a 60 wt% ethanol solution, transfer the mixture into an ultrasonic-microwave extractor, extract at 60°C, 60 W, and 700 W for 12 min, and centrifuge to obtain an alcohol extract and a precipitate.

[0042] S2, adding the precipitate obtained in S1 to petroleum ether at an addition amount of 0.12 g / mL, reflux extraction at 70°C for 3 h, drying to obtain defatted dry powder, adding the defatted dry powder to deionized water at an addition amount of 0.4 g / mL, ultrasonic extraction at 60°C and 160W for 40 min, centrifuging, taking the supernatant, concentrating, and precipitating with alcohol to obtain Lentinan;

[0043] S3. Add Lactobacillus rhamnosus powder to the supernatant obtained in S1 to obtain a bacterial solution, add whey protein at an addition amount of 0.08 g / mL to deionized water, add Lentinan to obtain a cross-linked solution, add the bacterial solution to the cross-linked solution, stir at 800 rpm for 1.5 h, spray dry, and obtain a composite microcapsule probiotic.

[0044] The preparation method of the dissolution-promoting polypeptide specifically comprises the following steps:

[0045] (1) adding pea protein powder to deionized water to prepare a 10 wt% pea protein solution, adjusting the pH to 9 with a 1 mol / L NaOH solution, and adding alkaline protease at a mass fraction of 1.0% of the pea protein solution to obtain a mixed solution;

[0046] (2) The mixed solution obtained in step (1) was hydrolyzed by shaking in a water bath at 60° C. for 4 h, the pH value was maintained constant with 1 mol / L NaOH solution, the pH was adjusted to 7.0, and the enzyme was inactivated at 95° C. for 10 min to obtain pea polypeptide;

[0047] (3) Add pea polypeptide to deionized water, add glucose, mix well, stir at 95°C oil bath at 120 rpm for 6 h, cool and dry to obtain dissolution-promoting polypeptide.

[0048] The present invention also provides a method for preparing a sheep colostrum composite powder for improving immunity, which specifically comprises the following steps:

[0049] Add composite microcapsule probiotics, goat colostrum dry powder, dissolution-promoting polypeptide, vitamin A, vitamin D, ascorbic acid, vitamin K1, vitamin B2 and folic acid into a milk powder mixing tank and mix them evenly to obtain goat colostrum composite powder for improving immunity.

[0050] Example 2

[0051] A sheep colostrum composite powder for improving immunity comprises the following components in parts by weight: 15 parts of composite microcapsule probiotics, 200 parts of sheep colostrum dry powder, 20 parts of dissolution-promoting polypeptides, 0.05 parts of vitamin A, 0.003 parts of vitamin D, 0.008 parts of ascorbic acid, 0.003 parts of vitamin K1, 0.006 parts of vitamin B2, and 0.002 parts of folic acid.

[0052] The composite microcapsule probiotics comprises the following components in parts by weight: 30 parts of shiitake mushrooms, 2.5 parts of whey protein and 0.3 parts of lactobacillus rhamnosus powder.

[0053] The dissolution-promoting polypeptide comprises the following components in parts by weight: 25 parts of pea protein powder and 10 parts of glucose.

[0054] The preparation method of composite microcapsule probiotics specifically comprises the following steps:

[0055] S1. Wash, freeze-dry, crush and sieve the mushrooms through a 100-mesh sieve to obtain mushroom powder. Add 0.04 g / mL of the mushroom powder to a 60 wt% ethanol solution, transfer the mixture into an ultrasonic-microwave extractor, perform 50°C 50 W ultrasonic and 600 W microwave extraction for 10 min, and centrifuge to obtain an alcohol extract and a precipitate.

[0056] S2, adding the precipitate obtained in S1 to petroleum ether at an addition amount of 0.1 g / mL, reflux extraction at 65°C for 2 h, drying to obtain defatted dry powder, adding the defatted dry powder to deionized water at an addition amount of 0.2 g / mL, ultrasonic extraction at 50°C and 150W for 30 min, centrifuging, taking the supernatant, concentrating, and precipitating with alcohol to obtain Lentinan;

[0057] S3. Add Lactobacillus rhamnosus powder to the supernatant obtained in S1 to obtain a bacterial solution, add whey protein at an addition amount of 0.05 g / mL to deionized water, add Lentinan to obtain a cross-linked solution, add the bacterial solution to the cross-linked solution, stir at 700 rpm for 1 h, spray dry, and obtain a composite microcapsule probiotic.

[0058] The preparation method of the dissolution-promoting polypeptide specifically comprises the following steps:

[0059] (1) adding pea protein powder to deionized water to obtain a 7 wt% pea protein solution, adjusting the pH to 8 with a 1 mol / L NaOH solution, and adding alkaline protease at a mass fraction of 1.0% of the pea protein solution to obtain a mixed solution;

[0060] (2) The mixed solution obtained in step (1) was hydrolyzed by shaking in a water bath at 50° C. for 3 h, the pH value was maintained constant with 1 mol / L NaOH solution, the pH was adjusted to 7.0, and the enzyme was inactivated at 95° C. for 10 min to obtain pea polypeptide;

[0061] (3) Add pea polypeptide to deionized water and glucose, mix well, stir in a 90°C oil bath at 60 rpm for 4 h, cool and dry to obtain a dissolution-promoting polypeptide.

[0062] The present invention also provides a method for preparing a sheep colostrum composite powder for improving immunity, which specifically comprises the following steps:

[0063] Add composite microcapsule probiotics, goat colostrum dry powder, dissolution-promoting polypeptide, vitamin A, vitamin D, ascorbic acid, vitamin K1, vitamin B2 and folic acid into a milk powder mixing tank and mix them evenly to obtain goat colostrum composite powder for improving immunity.

[0064] Example 3

[0065] A sheep colostrum composite powder for improving immunity comprises the following components in parts by weight: 18 parts of composite microcapsule probiotics, 250 parts of sheep colostrum dry powder, 22.5 parts of dissolution-promoting polypeptides, 0.007 parts of vitamin A, 0.005 parts of vitamin D, 0.009 parts of ascorbic acid, 0.004 parts of vitamin K1, 0.008 parts of vitamin B2, and 0.003 parts of folic acid.

[0066] The composite microcapsule probiotic comprises the following components in parts by weight: 35 parts of shiitake mushrooms, 3 parts of whey protein and 0.3-0.5 parts of lactobacillus rhamnosus powder.

[0067] The dissolution-promoting polypeptide comprises the following components in parts by weight: 28 parts of pea protein powder and 11 parts of glucose.

[0068] The preparation method of composite microcapsule probiotics specifically comprises the following steps:

[0069] S1. Wash, freeze-dry, crush and sieve the mushrooms through a 100-mesh sieve to obtain mushroom powder. Add 0.05 g / mL of the mushroom powder to a 60 wt% ethanol solution, transfer the mixture into an ultrasonic-microwave extractor, extract at 55°C, 55 W, and 650 W for 11 min, and centrifuge to obtain an alcohol extract and a precipitate.

[0070] S2, adding the precipitate obtained in S1 to petroleum ether at an addition amount of 0.11 g / mL, reflux extraction at 68°C for 2.5 h, drying to obtain defatted dry powder, adding the defatted dry powder to deionized water at an addition amount of 0.3 g / mL, ultrasonic extraction at 55°C 155w for 35 min, centrifuging, taking the supernatant, concentrating, and precipitating with alcohol to obtain Lentinan;

[0071] S3. Add Lactobacillus rhamnosus powder to the supernatant obtained in S1 to obtain a bacterial solution, add whey protein at an addition amount of 0.07 g / mL to deionized water, add Lentinan to obtain a cross-linked solution, add the bacterial solution to the cross-linked solution, stir at 750 rpm for 1.3 h, spray dry, and obtain a composite microcapsule probiotic.

[0072] The preparation method of the dissolution-promoting polypeptide specifically comprises the following steps:

[0073] (1) adding pea protein powder to deionized water to prepare an 8 wt% pea protein solution, adjusting the pH to 8.3 with a 1 mol / L NaOH solution, and adding alkaline protease at a mass fraction of 1.0% of the pea protein solution to obtain a mixed solution;

[0074] (2) The mixed solution obtained in step (1) was hydrolyzed by shaking in a water bath at 55°C for 3.5 hours, the pH value was maintained constant with 1 mol / L NaOH solution, the pH was adjusted to 7.0, and the enzyme was inactivated at 95°C for 10 minutes to obtain pea polypeptide;

[0075] (3) Add pea polypeptide to deionized water and glucose, mix well, stir at 90 rpm in an oil bath at 92.5°C for 5 h, cool and dry to obtain a dissolution-promoting polypeptide.

[0076] The present invention also provides a method for preparing a sheep colostrum composite powder for improving immunity, which specifically comprises the following steps:

[0077] Add composite microcapsule probiotics, goat colostrum dry powder, dissolution-promoting polypeptide, vitamin A, vitamin D, ascorbic acid, vitamin K1, vitamin B2 and folic acid into a milk powder mixing tank and mix them evenly to obtain goat colostrum composite powder for improving immunity.

[0078] Comparative Example 1

[0079] This comparative example provides a sheep colostrum composite powder, which differs from Example 1 only in that the powder does not contain Lentinan, and the remaining components and component contents are the same as those in Example 1.

[0080] No comparison example 2

[0081] This comparative example provides a sheep colostrum composite powder, which differs from Example 1 only in that it does not contain lentinan and whey protein, and the remaining components and component contents are the same as those in Example 1.

[0082] Comparative Example 3

[0083] This comparative example provides a sheep colostrum composite powder, which differs from Example 1 only in that it does not contain glucose, and the other components and component contents are the same as those in Example 1.

[0084] Experimental example

[0085] 1. Digestion experiment

[0086] Artificial gastric juice was prepared by dissolving 3 g of pepsin in 1000 mL of 0.5% (w / v) NaCl solution and adjusting the pH to 2;

[0087] The composite powders obtained in Examples 1-3 and Comparative Examples 1-2 were added to artificial gastric juice, and the gastric emptying time was 4 hours, and the mixture was shaken at 100 rpm under 37° C. water bath conditions. The rhamnosus content was measured at 0 hours and 4 hours, respectively. The content at 0 hours was taken as the reference value, and 4 hours was taken as the final value. The preservation rate was calculated using the following formula:

[0088] Preservation rate = final value / baseline value × 100%.

[0089] Figure 1 This is a result diagram of the digestion experiment of Examples 1-3 of the present invention and Comparative Example 1-2. As shown in the figure, the preservation rates of Bacillus rhamnosus of Examples 1-3 and Comparative Example 1-2 are 93.6%, 92.5%, 93.3%, 76.3% and 56.4%, respectively; the preservation rate of Bacillus rhamnosus of Example 1-3 is significantly higher than that of Comparative Example 1-2, indicating that the use of Lentinan and the preparation of microcapsules reduce the damage of gastric juice to Bacillus rhamnosus and play a protective role.

[0090] 2. Enteric Coating Test

[0091] Artificial intestinal fluid was prepared by dissolving 10 g of trypsin and 3 g of ox bile salt in 1 L of 0.5% NaCl solution and adjusting the pH to 7.5 with 0.1 mol / L NaOH solution.

[0092] The composite powders obtained in Examples 1-3 were respectively subjected to gastric juice digestion experiments, and then added to artificial intestinal juice, shaken at 37°C and 100 rpm for 6 hours to simulate the intestinal digestion process. The rhamnosus content was measured at 0 hours and 6 hours, respectively. The content at 0 hours was taken as the reference value, and 6 hours was taken as the final value. The release rate of rhamnosus was calculated by the following formula:

[0093] Release rate = (baseline value - final value) / baseline value × 100%.

[0094] Figure 2 This is a graph showing the results of the enteric coating experiment of Examples 1-3 of the present invention. As shown in the figure, the release rates of Examples 1-3 are 88.1%, 88.2%, and 86.3%, respectively; the release rates of Bacillus rhamnosus in Examples 1-3 are all greater than 85%, indicating that the Bacillus rhamnosus in the microcapsules has a good release effect in the intestine.

[0095] 3. Precipitation Experiment

[0096] The composite powders obtained in Examples 1-3 and Comparative Example 3 were added into deionized water at 40° C. at an addition amount of 0.1 g / ml, stirred at 80 rpm for dissolution, and the precipitation time was recorded;

[0097] Figure 3 It is a result diagram of the precipitation experiment of Examples 1-3 and Comparative Example 3 of the present invention. As shown in the figure, the time for precipitation to occur in Examples 1-3 and Comparative Example 3 is 7.6 min, 7.5 min, 7.6 min, and 3.8 min, respectively; the use of glucose in Examples 1-3 prolongs the time for precipitation to occur.

[0098] 4. Dissolution experiment

[0099] The composite powders obtained in Examples 1-3 and Comparative Example 3 were added to deionized water at 40°C at an addition amount of 0.1 g / ml, shaken evenly, centrifuged at 3000 r / min for 5 min, and 25 mL of the supernatant was taken and dried at 105°C. The mass of dry matter in the supernatant was recorded as the dissolved mass, and the mass of the composite powder added to the solution was taken as the total mass. The dissolution rate was calculated using the following formula:

[0100] Dissolution rate = dissolved mass / total mass × 100%.

[0101] Figure 4It is a result diagram of the dissolution experiment of Examples 1-3 and Comparative Example 3 of the present invention. As shown in the figure, the dissolution rates of Examples 1-3 and Comparative Example 3 are 79.6%, 78.9%, 79.3%, and 63.1%, respectively; the use of glucose in Examples 1-3 improves the dissolution rate.

[0102] 5. Agglomeration Experiment

[0103] The composite powder obtained in Examples 1-3 and Comparative Example 3 was added to deionized water at 40° C. in an amount of 0.2 g / ml, stirred in one direction at 10 rpm to dissolve, and filtered through a 60-mesh sieve. The dry weight of the substance obtained by filtration was taken as the mass of the agglomerates, and the mass of the composite powder added to the solution was taken as the total mass. The agglomeration rate was calculated by the following formula:

[0104] Agglomeration rate = agglomeration mass / total mass × 100%.

[0105] Figure 5 It is a result diagram of the dissolution experiment of Examples 1-3 and Comparative Example 3 of the present invention. As shown in the figure, the agglomeration rates of Examples 1-3 and Comparative Example 3 are 13.5%, 13.9%, 12.1%, and 28.7%, respectively; the use of glucose in Examples 1-3 improves the dissolution rate.

[0106] Figure 6 This is an electron microscope picture of the composite microcapsule probiotics in Example 1 of the present invention. As shown in the figure, the microcapsules have good morphology, uniform size, and complete encapsulation.

[0107] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention.

[0108] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A sheep colostrum composite powder composition for improving immunity, characterized in that: The invention comprises the following components in parts by weight: 15-20 parts of composite microcapsule probiotics, 200-300 parts of goat colostrum dry powder, 20-25 parts of dissolution-promoting polypeptides, 0.005-0.009 parts of vitamin A, 0.003-0.006 parts of vitamin D, 0.008-0.01 parts of ascorbic acid, 0.003-0.005 parts of vitamin K1, 0.006-0.009 parts of vitamin B2, and 0.002-0.004 parts of folic acid; the composite microcapsule probiotics are lentinan-whey protein microcapsules encapsulating rhamnosus lactobacillus; the composite microcapsule probiotics comprises the following components in parts by weight: 30-40 parts of shiitake mushrooms, 2.5-3.5 parts of whey protein, and 0.3-0.5 parts of rhamnosus lactobacillus powder; the dissolution-promoting polypeptide comprises the following components in parts by weight: 25-30 parts of pea protein powder and 10-12 parts of glucose.

2. A method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 1, characterized in that: The method specifically comprises the following steps: adding composite microcapsule probiotics, goat colostrum dry powder, dissolution-promoting polypeptide, vitamin A, vitamin D, ascorbic acid, vitamin K1, vitamin B2 and folic acid into a milk powder mixing tank and mixing them evenly to obtain goat colostrum composite powder for improving immunity.

3. The method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 2, characterized in that: The preparation method of the composite microcapsule probiotics specifically comprises the following steps: S1, washing, freeze-drying, crushing and passing through a 100-mesh sieve to obtain mushroom powder, adding the mushroom powder to a 60wt% ethanol solution, transferring the solution to an ultrasonic-microwave extractor, performing ultrasonic-microwave extraction, and centrifuging to obtain a supernatant and a precipitate; S2, adding the precipitate obtained in S1 into petroleum ether, reflux extraction, drying, adding into deionized water, ultrasonic extraction, centrifugation, taking the supernatant, concentrating, and ethanol precipitation to obtain Lentinan; S3. Add Lactobacillus rhamnosus powder to the supernatant obtained in S1 to obtain a bacterial solution, add whey protein to deionized water, add Lentinan to obtain a cross-linked solution, add the bacterial solution to the cross-linked solution, stir at 700-800 rpm for 1-1.5 h, spray dry, and obtain a composite microcapsule probiotic.

4. The method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 3, characterized in that: In S1, the shiitake mushroom is added to a 60 wt % ethanol solution in an amount of 0.04-0.06 g / mL.

5. The method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 4, characterized in that: In S1, the ultrasonic microwave extraction has a temperature of 40-50°C, an acoustic power of 50-60W, a microwave power of 600-700W, and a time of 10-12min.

6. The method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 5, characterized in that: In S2, the amount of the precipitate obtained in S1 added to petroleum ether is 0.1-0.12 g / mL.

7. The method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 6, characterized in that: In S2, the reflux extraction temperature is 65-70°C and the time is 2-3h.

8. The method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 7, characterized in that: In S2, the amount of the defatted dry powder added to deionized water is 0.2-0.4 g / mL; the ultrasonic extraction temperature is 50-60° C., the ultrasonic power is 150-160 w, and the time is 30-40 min.

9. The method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 8, characterized in that: In S3, the whey protein is added in an amount of 0.05-0.08 g / mL in deionized water.

10. The method for preparing the immunity-enhancing sheep colostrum composite powder composition according to claim 9, characterized in that: The preparation method of the dissolution-promoting polypeptide specifically comprises the following steps: (1) adding pea protein powder to deionized water to obtain a 7-10 wt% pea protein solution, adjusting the pH to 8-9 with a 1 mol / L NaOH solution, and adding alkaline protease at a mass fraction of 1.0% of the pea protein solution to obtain a mixed solution; (2) The mixed solution obtained in step (1) was hydrolyzed by shaking in a water bath at 50-60° C. for 3-4 hours, the pH value was maintained constant with 1 mol / L NaOH solution, the pH was adjusted to 7.0, and the enzyme was inactivated at 95° C. for 10 minutes to obtain pea polypeptide; (3) Add pea polypeptide to deionized water, add glucose, mix well, stir at 60-120 rpm in an oil bath at 90-95°C for 4-6 hours, cool and dry to obtain a dissolution-promoting polypeptide.

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