Method for improving quality of fermented cigar tobacco leaves by using composite biological enzyme preparation

Abstract

The invention provides a method for improving the quality of fermented cigar tobacco leaves through a composite biological enzyme preparation, and relates to the technical field of tobacco production. According to the method, the accumulation of polyphenol metabolites in the cigar tobacco leaf fermentation process is promoted through common auxiliary fermentation of the fiveleaf gynostemma herb extracting solution, cellulase and neutral protease, so that chemical components in the tobacco leaf fermentation process are more coordinated, and the chemical quality and aroma quality of the fermented tobacco leaves are improved.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco production, and in particular to a method for improving the quality of fermented cigar tobacco leaves using a composite bio-enzyme preparation. Background Art

[0002] Cigar is a tobacco product that is made of whole tobacco leaves and is hand-rolled after a modulation and fermentation process. Compared with other tobacco products, the characteristics of cigars are mainly manifested in rich and deep aroma, mellow smoke and strong sensory satisfaction. Cigarette wrapper, as the outermost wrapping layer of the cigar structure, weighs about 10% of the total weight of the tobacco leaves. The selection standards for cigar wrapper tobacco leaves are extremely high, requiring the tobacco leaves to be complete and undamaged, uniform in color and shiny, and the side veins to be delicate and not prominent. The wrapper tobacco leaves bear the core function of stabilizing and restraining the cigar core tobacco leaves. Its proportion in the total tobacco leaf mass of the cigar is generally 15%. Its combustion efficiency and uniformity largely determine the rhythmicity of the overall burning of the cigar and the compactness of the rolling. The cigar core tobacco leaves are located in the center of the cigar, and its mass can account for up to 75% of the entire cigar, which plays a decisive role in the overall quality of the cigar. The filler tobacco should exhibit the typical flavor characteristics of cigars, be rich in diverse aromas, and have excellent smoldering durability, thus ensuring that the overall smoking experience of the cigar reaches a high level.

[0003] After being harvested and dried, cigar tobacco leaves often have some defects in terms of physical properties and internal quality, which are manifested as appearance problems such as green spots and uneven color on the leaf surface, accompanied by a significant green odor, a strong irritating taste, and the leaves are easy to break and have poor burning properties. Therefore, a certain degree of fermentation and aging is required to achieve the overall optimization of the quality of cigar tobacco leaves and meet the needs of industrial production.

[0004] At present, the quality of tobacco leaves is mainly improved by adding biological enzymes, chemical additives and microorganisms during the baking or purification process. Although the above methods can improve the quality of tobacco leaves, there are still problems such as heavy green smell and lack of aroma. It is necessary to further improve the quality of tobacco leaves after fermentation. Therefore, it is necessary to provide a new method for improving the quality of cigar tobacco leaves. Summary of the invention

[0005] The purpose of the present invention is to provide a method for improving the quality of cigar tobacco leaves after fermentation by using a composite bio-enzyme preparation in view of the deficiencies of the prior art. The present invention treats tobacco leaves with a Gynostemma pentaphyllum water extract and a bio-enzyme, so that the chemical components of the tobacco leaves during fermentation are more coordinated, thereby improving the chemical quality and aroma quality of the tobacco leaves after fermentation.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] A method for improving the quality of fermented cigar tobacco leaves by using a composite bioenzyme preparation comprises: mixing a Gynostemma pentaphyllum extract with cellulase and neutral protease to obtain the composite bioenzyme preparation, and spraying the composite bioenzyme preparation on the surface of the cigar tobacco leaves for treatment.

[0008] Furthermore, the Gynostemma pentaphyllum extract is prepared by the following method:

[0009] Add water to Gynostemma pentaphyllum tea leaves and boil them for extraction. The mass ratio of Gynostemma pentaphyllum tea leaves to water is 1:(150-200). The extraction temperature is 70-80°C. The extraction time is 40-80 minutes. Filter by suction at room temperature to obtain Gynostemma pentaphyllum extract.

[0010] Preferably, in the preparation process of the Gynostemma pentaphyllum extract, the mass ratio of Gynostemma pentaphyllum tea leaves to water is 1:200, the extraction temperature is 75° C., and the extraction time is 60 min.

[0011] Furthermore, the contents of the components in the composite bio-enzyme preparation are as follows: 40-80 g / mL of Gynostemma pentaphyllum extract, 70-170 U / g of cellulase, and 70-170 U / g of neutral protease.

[0012] Furthermore, in the composite bio-enzyme preparation, the content of Gynostemma pentaphyllum extract in the Gynostemma pentaphyllum extract can be selected from 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 60, 70, 80 or any range therebetween; the content of cellulase can be selected from 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170 U / g or any range therebetween; the content of neutral protease can be selected from 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170 U / g or any range therebetween.

[0013] Preferably, the contents of the components in the composite bio-enzyme preparation are: 50-70 g / mL of Gynostemma pentaphyllum extract, 80-170 U / g of cellulase, and 80-160 U / g of neutral protease.

[0014] More preferably, the contents of the components in the composite bio-enzyme preparation are: 50 g / mL of Gynostemma pentaphyllum extract, 140 U / g of cellulase, and 120 U / g of neutral protease.

[0015] Furthermore, the mass ratio of the composite bio-enzyme preparation to cigar tobacco is 1:(5-15).

[0016] Furthermore, the method for improving the quality of cigar tobacco leaves after fermentation by using the composite bio-enzyme preparation also includes the following steps: after the cigar tobacco leaves sprayed with the composite bio-enzyme preparation are moistened, they are piled up and fermented.

[0017] Preferably, the temperature of the stacking fermentation is room temperature and the humidity is 65-80%.

[0018] Preferably, the stacking fermentation time is 50 to 60 days.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The present invention provides a method for improving the quality of cigar tobacco leaves after fermentation by using a composite bio-enzyme preparation, which promotes the accumulation of polyphenol metabolites during the fermentation of cigar tobacco leaves by co-fermentation with a Gynostemma pentaphyllum extract, cellulase and neutral protease, thereby making the chemical composition of the tobacco leaves more coordinated during the fermentation process and improving the chemical quality and aroma quality of the tobacco leaves after fermentation.

[0021] 2. The method of improving the quality of cigar tobacco leaves after fermentation by using a composite bio-enzyme preparation of the present invention, wherein the Gynostemma pentaphyllum extract interacts with cellulase and neutral protease, which helps to activate the activity of the inherent enzymes in the cigar tobacco leaves, thereby slowing down the degradation and conversion of polyphenols during tobacco leaf fermentation, increasing the activity of key enzymes PPO, POD and PAL of polyphenol metabolites during tobacco leaf fermentation, slowing down the consumption of carbohydrate compounds, and increasing the degradation and conversion rate of nitrogen-containing compounds; increasing the accumulation of polyphenols, malic acid and non-volatile organic acids in the fermented tobacco leaves. The total sugar, rutin, chlorogenic acid, total flavonoids, total phenols and malic acid in the tobacco leaves are increased, the nicotine, total nitrogen, protein and citric acid content are reduced, the chemical quality of the tobacco leaves is improved, and the chemical composition is more coordinated.

[0022] 3. The method for improving the quality of cigar tobacco leaves after fermentation by using the composite bio-enzyme preparation of the present invention helps to increase the content of neutral aroma substances in tobacco leaves (except phytol) by controlling the preparation conditions of the Gynostemma pentaphyllum extract, and increases the content of phenylalanine degradation products, carotenoid degradation products, cypermethrin-like degradation products, chlorophyll degradation products, etc.; among them, the contents of phenylacetaldehyde, indole, and β-damasconone are the highest, and the total content of megastigmatrienone, solanone, phytol, and other aroma substances are significantly increased. DETAILED DESCRIPTION

[0023] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is only an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the invention of the present invention based on the disclosed content, and they should also fall within the scope of the present invention.

[0024] The present invention is further described below by way of specific examples. Unless otherwise specified, the various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels.

[0025] Examples 1-12

[0026] A method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation comprises the following steps:

[0027] The compound bio-enzyme preparation is prepared by mixing the Gynostemma pentaphyllum extract with cellulase and neutral protease, and then spraying the mixture on the surface of cigar tobacco leaves at a mass ratio of 1:10 for treatment. After the cigar tobacco leaves are moistened, they are piled up and fermented at room temperature and a humidity of 70% for 60 days.

[0028] The specific components and dosage of the composite bio-enzyme preparation are shown in Table 1 below.

[0029] The Gynostemma pentaphyllum extract was prepared by the following method:

[0030] Add water to Gynostemma pentaphyllum tea leaves and boil them for extraction. The mass ratio of Gynostemma pentaphyllum tea leaves to water is 1:200. The extraction temperature is 75°C and the extraction time is 60 minutes. Filter with suction at room temperature to obtain Gynostemma pentaphyllum extract.

[0031] Table 1

[0032]

[0033]

[0034] Comparative Examples 1-4

[0035] The difference from Example 2 is that the composition of the composite bio-enzyme preparation is different, as shown in Table 2 below.

[0036] Table 2

[0037] Group Gynostemma pentaphyllum extract g / mL Cellulase U / g Neutral protease U / g α-Amylase Comparative Example 1 —— 140 120 —— Comparative Example 2 50 —— 120 —— Comparative Example 3 50 140 —— —— Comparative Example 4 50 140 —— 120

[0038] Test example

[0039] The present invention takes QX103 middle tobacco leaves from Tonggousi Town, Mian County, Hanzhong City, Shaanxi Province as an example, and treats them by the methods provided in the above embodiments and comparative examples, and uses untreated tobacco leaves as a control to determine the changes in the main chemical components, polyphenols, non-volatile organic acids, free amino acids, neutral aroma substances and enzyme activities in the treated tobacco leaves and untreated tobacco leaves (CK group). The test results are shown in Tables 3-9 below.

[0040] Table 3. Content of main chemical components

[0041]

[0042]

[0043] The above table shows that the content of the main chemical components of cigar tobacco leaves fermented by the method of the present invention has changed significantly: compared with CK, the total sugar, reducing sugar, total nitrogen, and potassium content of Example 2 increased significantly, the nicotine content decreased, and the chlorine content changed significantly. The treatment effect of this group is better. In Comparative Examples 1-3, only two of the components were added to the composite enzyme biological preparation, and the content of the main chemical components of the cigar tobacco leaves after treatment changed, but the treatment effect was obviously not as good as that of Example 2. In Comparative Example 4, the neutral protease in the composite enzyme biological preparation was replaced with α-amylase, and its effect on cigar tobacco leaves was not obvious.

[0044] In Examples 1-4, Example 2 has the best effect, followed by Example 3. It can be seen that when the content of the Gynostemma pentaphyllum water extract is 50-70 g / mL, the effect on cigar tobacco leaves is better. In Examples 5-8, the total sugar, reducing sugar, total nitrogen, and potassium contents of Example 7 are significantly increased, the nicotine content is reduced, and the chlorine content changes significantly. The treatment effect of Example 7 is better, and the treatment effect of Example 6 is second, indicating that when the cellulase content is in the range of 80-160 U / g, the effect on cigar tobacco leaves is better. In Examples 9-12, the total sugar, reducing sugar, total nitrogen, and potassium contents of Example 11 are significantly increased, the nicotine content is reduced, and the chlorine content changes significantly. The treatment effect of Example 11 is better, and Example 10 is second. It can be seen that when the cellulase content is in the range of 80-160 U / g, the effect on cigar tobacco leaves is better.

[0045] The above results show that the combination of Gynostemma pentaphyllum extract, cellulase and neutral protease has a certain synergistic effect, which makes the chemical composition of tobacco leaves more coordinated during fermentation and improves the quality of tobacco leaves; the appropriate proportion of Gynostemma pentaphyllum water extract and biological enzyme application can slow down the degradation and conversion of total sugar in cigar tobacco fermentation, and has an effect on improving the total sugar content of tobacco leaves after cigar fermentation. The effect is better when the formula of composite enzyme biological preparation is: 50g / mL Gynostemma pentaphyllum + 140U / g cellulase + 120U / g neutral protease.

[0046] Table 4. Polyphenol content (mg / g)

[0047] Group Chlorogenic acid Rutin Total flavonoids Total phenols CK group 0.175 0.351 2.210 5.201 Example 1 0.197 0.374 2.74 6.212 Example 2 0.204 0.462 3.00 6.413 Example 3 0.198 0.464 2.84 6.304 Example 4 0.192 0.478 2.78 6.327 Example 5 0.180 0.425 2.32 5.781 Example 6 0.188 0.442 2.41 5.991 Example 7 0.211 0.451 2.94 6.225 Example 8 0.194 0.457 2.92 6.203 Example 9 0.183 0.379 2.23 5.845 Example 10 0.186 0.441 2.74 6.019 Embodiment 11 0.192 0.466 3.14 6.377 Example 12 0.187 0.459 3.10 6.244 Comparative Example 1 0.197 0.366 2.47 6.184 Comparative Example 2 0.181 0.401 2.25 5.662 Comparative Example 3 0.185 0.357 2.20 5.743 Comparative Example 4 0.177 0.355 2.22 5.379

[0048] The above table shows the changes in the content of polyphenols in cigar tobacco leaves after fermentation by the method of the present invention: compared with CK, the total chlorogenic acid, rutin, total flavonoids, and total phenol content of Example 2 are significantly increased, and the treatment effect of Example 2 is better. Analysis of Comparative Examples 1-4 shows that the addition of two components in Gynostemma pentaphyllum extract, cellulase, and neutral protease can significantly change the content of polyphenols, but the content of polyphenols is significantly lower than that of Example 2, and in Comparative Example 4, the neutral protease is replaced by α-amylase, and its polyphenol content has no significant change compared with the CK group, that is, its treatment effect on cigar tobacco leaves is not obvious.

[0049] In Examples 1-4, the total chlorogenic acid, rutin, total flavonoids, and total phenol contents of Example 2 were significantly increased, and Example 2 had the best treatment effect, followed by Example 3. In Examples 5-8, the total chlorogenic acid, rutin, total flavonoids, and total phenol contents of Example 7 were significantly increased, followed by Example 6. In Examples 9-12, the total chlorogenic acid, rutin, total flavonoids, and total phenol contents of Example 11 were significantly increased, and Example 11 had the best treatment effect, followed by Example 10.

[0050] The above results show that the combination of Gynostemma pentaphyllum extract, cellulase and neutral protease has a certain synergistic effect, especially when applied in a specific compound ratio, it can slow down the degradation of total chlorogenic acid, rutin, total flavonoids and total phenols during cigar tobacco fermentation, and increase the content of polyphenols in tobacco leaves after cigar fermentation. The effect is better when 50g / mL Gynostemma pentaphyllum + 140U / g cellulase + 120U / g neutral protease are applied.

[0051] Table 5. Non-volatile organic acid content (unit: mg / g)

[0052] Group Malic Acid Malonic acid Citric Acid Succinic acid Total CK group 110.44 2.01 81.60 0.514 199.514 Example 1 111.30 2.14 84.34 0.542 203.092 Example 2 119.72 2.45 87.72 0.507 214.717 Example 3 115.20 2.12 90.70 0.546 213.516 Example 4 112.51 2.37 84.07 0.434 204.154 Example 5 110.54 2.28 84.71 0.486 202.336 Example 6 111.72 2.35 83.96 0.557 203.537 Example 7 117.36 2.39 106.17 0.550 231.24 Example 8 115.91 2.34 94.62 0.491 217.681 Example 9 111.76 2.31 96.38 0.521 215.921 Example 10 113.20 2.42 95.72 0.518 216.628 Embodiment 11 117.37 2.54 98.64 0.536 223.406 Example 12 116.32 2.47 97.86 0.527 222.127 Comparative Example 1 110.97 2.22 80.33 0.531 198.821 Comparative Example 2 110.48 2.13 82.19 0.487 199.607 Comparative Example 3 111.46 2.29 88.66 0.519 207.879 Comparative Example 4 110.46 2.11 82.04 0.518 199.898

[0053] The above table shows the changes in the non-volatile organic acid content of cigar tobacco leaves after fermentation by the method of the present invention: compared with CK, the malic acid, malonic acid, citric acid, and succinic acid contents of Example 2 are significantly increased, and the treatment effect of this group is better. In Examples 1-3, two components of Gynostemma pentaphyllum extract, cellulase, and neutral protease are added respectively, and several components of malic acid, malonic acid, citric acid, or succinic acid in the tobacco leaves after treatment increase, and the total content of non-volatile organic acids is significantly lower than that of Example 2; while in Comparative Example 4, the neutral protease is replaced by α-amylase, and its non-volatile organic acid content does not change significantly compared with the CK group.

[0054] In Examples 1-4, Example 2 had the best treatment effect, followed by Example 3. In Examples 5-8, the contents of malic acid, malonic acid, citric acid, and succinic acid in Example 7 increased significantly, followed by Example 6. In Examples 9-12, the contents of malic acid, malonic acid, citric acid, and succinic acid in Example 11 increased significantly, with Example 11 having the best treatment effect, followed by Example 10.

[0055] The above results show that the combination of Gynostemma pentaphyllum extract, cellulase and neutral protease has a certain synergistic effect, especially when applied in a specific compound ratio, it can increase the content of non-volatile organic acids in cigar tobacco leaves.

[0056] Table 6. Content of neutral aroma substances in Examples 1-6 (in μg / g)

[0057]

[0058]

[0059] Table 7. Content of neutral aroma substances in Examples 7-12 (in μg / g)

[0060]

[0061]

[0062] Table 8. Content of neutral aroma substances in comparative examples 1-4 (unit: μg / g)

[0063]

[0064]

[0065] The above table shows the changes in the content of neutral aroma substances in cigar tobacco leaves fermented by the method of the present invention: Compared with CK, in Examples 1-4, the contents of phenylalanine degradation products, carotenoid degradation products, cypermethrin degradation products, and chlorophyll degradation products of Examples 1 and 2 were significantly increased, and the treatment effect of Example 2 was better. In Examples 5-8, the contents of phenylalanine degradation products, carotenoid degradation products, cypermethrin degradation products, and chlorophyll degradation products of Examples 5 and 7 were significantly increased, and the treatment effect of Example 7 was better. In Examples 9-12, the contents of phenylalanine degradation products, carotenoid degradation products, cypermethrin degradation products, and chlorophyll degradation products of Examples 10 and 11 were significantly increased, and the treatment effect of Example 10 was better, followed by Example 11. Comparative Examples 1, 2 and 3 were analyzed. Adding Gynostemma pentaphyllum extract, cellulase and neutral protease respectively can significantly change the content of neutral aroma substances. The contents of phenylacetaldehyde, indole and β-damasketone are the highest, and the total content of megastigmatrienone, solanone, phytol and other aroma substances are significantly increased. However, when α-amylase is added to Comparative Example 4, the treatment effect is not obvious. Based on the above results, the appropriate proportion of Gynostemma pentaphyllum water extract and biological enzyme application can improve the content of neutral aroma substances in cigar tobacco leaves.

[0066] Table 9. Enzyme activity

[0067]

[0068] The above table shows the changes in the activities of enzymes related to polyphenol metabolism in cigar tobacco leaves fermented by the method of the present invention: Compared with CK, in Examples 1-4, the activities of PPO, PAL and POD in Examples 2 and 4 were significantly improved, with Example 4 having a better treatment effect, followed by Example 2; Compared with Comparative Example 1, adding Gynostemma pentaphyllum extract can significantly change the activities of enzymes related to polyphenol metabolism. In Examples 5-8, the activities of PPO, PAL and POD in Examples 6, 7 and 8 were significantly improved, with Example 7 having a better treatment effect, followed by Example 6. In Examples 9-12, the activities of PPO, PAL and POD in Examples 11 and 12 were significantly improved, with Example 11 having a better treatment effect, followed by Example 12. Comparative Examples 1, 2 and 3 were analyzed, and the addition of Gynostemma pentaphyllum extract, cellulase and neutral protease respectively can significantly change the conversion of polyphenols, and the activities of PPO, PAL and POD are significantly improved, while the addition of α-amylase in Comparative Example 3 has no obvious treatment effect.

[0069] In summary, the present invention can improve the activity of key enzymes PPO, POD and PAL of polyphenol metabolites in the process of tobacco leaf fermentation by treating tobacco leaves with gynostemma pentaphyllum extract, cellulase and neutral protease, and improve the degradation and conversion rate of nitrogen-containing compounds; increase the accumulation of polyphenols, malic acid and total amount of non-volatile organic acids in tobacco leaves after fermentation; increase the content of total sugar, rutin, chlorogenic acid, total flavonoids, total phenols and malic acid in tobacco leaves, reduce nicotine and total nitrogen, increase flavor substances, make flue gas more fragrant and mellow, and have rich taste. Comprehensively considering the content of main chemical components, polyphenols, non-volatile organic acids and other data of tobacco leaves after fermentation, when cigar tobacco leaves are treated with 50g / mL gynostemma pentaphyllum extract + 140U / g cellulase + 120U / g neutral protease, the effect is optimal.

[0070] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation, characterized in that: include: The composite bio-enzyme preparation prepared by mixing the Gynostemma pentaphyllum extract with cellulase and neutral protease is sprayed on the surface of cigar tobacco leaves for treatment.

2. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 1, characterized in that: The Gynostemma pentaphyllum extract is prepared by the following method: Add water to Gynostemma pentaphyllum tea leaves and boil them for extraction. The mass ratio of Gynostemma pentaphyllum tea leaves to water is 1:(150-200). The extraction temperature is 70-80°C. The extraction time is 40-80 minutes. Filter by suction at room temperature to obtain Gynostemma pentaphyllum extract.

3. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 2, characterized in that: In the preparation process of the Gynostemma pentaphyllum extract, the mass ratio of Gynostemma pentaphyllum tea leaves to water is 1:200, the extraction temperature is 75° C., and the extraction time is 60 min.

4. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 1, characterized in that: The contents of the components in the composite bio-enzyme preparation are as follows: 40-80 g / mL of gynostemma pentaphyllum extract, 70-170 U / g of cellulase, and 70-170 U / g of neutral protease.

5. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 4, characterized in that: The content of each component in the composite biological enzyme preparation is as follows: 50-70 g / mL of Gynostemma pentaphyllum extract, 80-170 U / g of cellulase, and 80-160 U / g of neutral protease.

6. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 5, characterized in that: The contents of the components in the composite bio-enzyme preparation are as follows: 50 g / mL of Gynostemma pentaphyllum extract, 140 U / g of cellulase, and 120 U / g of neutral protease.

7. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 1, characterized in that: The mass ratio of the composite bio-enzyme preparation to cigar tobacco leaves is 1:(5-15).

8. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 1, characterized in that: The method for improving the quality of fermented cigar tobacco leaves by using the composite bio-enzyme preparation also includes the following steps: after the cigar tobacco leaves sprayed with the composite bio-enzyme preparation are moistened, they are piled up and fermented.

9. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 8, characterized in that: The temperature of the stacking fermentation is room temperature, and the humidity is 65-80%.

10. The method for improving the quality of cigar tobacco leaves after fermentation using a composite bio-enzyme preparation according to claim 9, characterized in that: The stacking fermentation time is 50 to 60 days.

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