Red repairing and relieving composition as well as preparation method and application thereof

Abstract

The invention discloses a redness repairing and relieving composition as well as a preparation method and application thereof. The red repairing and relieving composition comprises a red repairing and relieving functional agent, wherein the components of the red repairing and relieving functional agent comprise a phenolic compound and a phenolic acid compound; the phenolic compound is selected from at least one of hydroxytyrosol and tyrosol; the phenolic acid compound is selected from at least one of caffeic acid, protocatechuic acid, plateau catechuic acid and ethyl protocatechuate. The composition can inhibit release of inflammatory factors, relieve itching, resist inflammation, calm skin and reduce angiogenesis, and meanwhile can enhance the skin water locking capacity, improve the skin water shortage condition and enable the skin to recover to be moisturized, tender and smooth.

Description

Technical Field

[0001] The present invention relates to the technical field of skin repair, and in particular to a redness-repairing and soothing composition, and a preparation method and application thereof. Background Art

[0002] As the largest organ in the human body, the skin plays an important role in protecting the human body. However, since the skin is in contact with the external environment all the time and is exposed to various stimuli (such as bacterial invasion, sun exposure, etc.), it is easy to develop various skin problems during the stress process.

[0003] At present, people lead a fast pace of life. Overtime, staying up late, fatigue, mental stress, etc. have become the norm. Coupled with irregular eating habits and bad skin care habits, the skin barrier will be seriously damaged, the skin's own repair ability will be reduced, and sensitive skin will be easily formed. Allergic skin is generally fragile. When the skin is exposed to trace or small amounts of irritants and allergens, it will become uncomfortable, intolerant, and inflammatory. The main manifestations are erythema, edema, desquamation, itching, tingling, and tightness on the skin. Therefore, how to provide a composition with soothing and redness repairing effects, so that it can enhance the skin barrier function and repair damaged skin, has become a problem that needs to be solved urgently.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The object of the present invention is to provide a redness repairing and soothing composition and its preparation method and application. The composition increases the skin repairing effect from the aspects of anti-inflammatory, antipruritic, soothing and anti-allergic, and has the characteristics of mild ingredients, safety and non-irritation.

[0006] The present invention is achieved in that:

[0007] In a first aspect, the present invention provides a redness-repairing and soothing composition, the ingredients of which include a redness-repairing and soothing efficacy agent;

[0008] The ingredients of the redness-repairing and soothing agent include phenolic compounds and phenolic acid compounds, with a mass ratio of 0.1-10:0.5-10;

[0009] The phenolic compound is selected from at least one of hydroxytyrosol and tyrosol;

[0010] The phenolic acid compound is selected from at least one of caffeic acid, protocatechuic acid, catechuic acid, and ester derivatives thereof. The ester derivatives may be caffeic acid ethyl ester, caffeic acid methyl ester, protocatechuic acid methyl ester, protocatechuic acid ethyl ester, catechuic acid ethyl ester, and the like.

[0011] Preferably, the redness-removing and soothing agent comprises phenolic compounds and phenolic acid compounds in a mass ratio of (0.1-10):(1-10). More preferably, the redness-removing and soothing agent comprises phenolic compounds and phenolic acid compounds in a mass ratio of (0.1-5):1.

[0012] The functional ingredients contained in the redness-removing and soothing agent of the present invention have significant antipruritic, anti-inflammatory, redness-removing and soothing effects, and after adopting the above preferred ratio, the effect of the redness-removing and soothing agent is better.

[0013] In an optional embodiment, the redness-repairing and soothing agent further comprises terpenoid compounds.

[0014] In an optional embodiment, the terpenoid compound is selected from at least one of squalane and squalene.

[0015] In an optional embodiment, the mass ratio of the terpenoid compounds to the phenolic compounds and phenolic acid compounds is (1-25): (0.1-10).

[0016] Furthermore, the mass ratio of the above-mentioned terpenoid compounds to the phenolic compounds is (10-25): (0.1-10).

[0017] In an optional embodiment, the mass ratio of terpenoid compounds to phenolic compounds and phenolic acid compounds is 1:25:0.1-10:0.5-10.

[0018] In an optional embodiment, the redness-repairing and soothing agent further comprises a glycoside compound;

[0019] In an optional embodiment, the glycoside compound is selected from at least one of gardenia glycoside and coconut glucoside.

[0020] In an optional embodiment, the mass ratio of the glycoside compound to the phenolic compound is (0.1-6): (0.1-10).

[0021] In an optional embodiment, the redness-repairing and soothing agent is selected from one or more of hydroxytyrosol, caffeic acid, protocatechuic acid, squalane, gardenia glycoside and coconut glucoside.

[0022] Preferably, the redness-repairing and soothing agent comprises a combination of hydroxytyrosol, caffeic acid, squalane and gardenia glycoside, or a combination of hydroxytyrosol, protocatechuic acid, squalane and gardenia glycoside, or a combination of hydroxytyrosol, caffeic acid, protocatechuic acid, squalane and gardenia glycoside.

[0023] After a large number of experimental screenings, it was found that phenolic compounds, phenolic acid compounds, terpenoid compounds, and glycoside compounds have synergistic effects, can relieve itching, anti-inflammatory, calm the skin, reduce vascular proliferation, repair redness and soothe the skin. When the phenolic compound is hydroxytyrosol and the phenolic acid compound is caffeic acid and / or protocatechuic acid, the repair effect of the prepared redness-removing and soothing agent is better.

[0024] In an optional embodiment, the redness-repairing and soothing composition further comprises amino acid compounds, fatty acids or their ester compounds, alcohol-soluble proteins, cosmetic excipients and solvents.

[0025] In an optional embodiment, the solvent includes an organic solvent and water.

[0026] In an optional embodiment, the moisturizing organic solvent is selected from at least one of mannitol, glycerol, and ethanol.

[0027] In an optional embodiment, the amino acid compound is selected from at least one of glycine, histidine, and cystine.

[0028] In the present invention, amino acid compounds are used because they are easily absorbed by the skin and interact with other components in the skin to keep the skin surface intact and moisturized, prevent moisture loss in the stratum corneum, and improve the anti-allergic ability of sensitive skin.

[0029] In an optional embodiment, the fatty acid or its ester compound is selected from at least one of myristic acid, palmitic acid, oleic acid, linolenic acid, linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid.

[0030] In the present invention, fatty acids or their ester compounds are used, and the fatty acids include saturated fatty acids and unsaturated fatty acids; among them, unsaturated fatty acids such as myristic acid, linolenic acid, and linoleic acid can resist oxidation, improve the condition of the skin, enhance skin resistance, and make the skin smooth, moisturized, and maintain elasticity.

[0031] In an optional embodiment, the alcohol-soluble protein is selected from at least one of wheat alcohol-soluble protein and zein.

[0032] In an optional embodiment, the cosmetic excipients include surfactants, stabilizers, and pH adjusters.

[0033] In an optional embodiment, the cosmetic auxiliary material further includes a preservative.

[0034] In an optional embodiment, the stabilizer is selected from at least one of Brunei gum and glucomannan.

[0035] In the present invention, alcohol-soluble protein and stabilizer can encapsulate active ingredients such as hydroxytyrosol, caffeic acid, protocatechuic acid, gardenia glycoside, etc., thereby improving the stability of the active ingredients. When not in use, the active ingredients can be stably present; when in use, after being applied to the skin, the active ingredients can be better absorbed through the skin with the cooperation of other components, and fully exert their effects, thereby achieving the effects of redness repair, soothing, moisturizing and nourishing.

[0036] In an optional embodiment, the surfactant is selected from at least one of lauryl alcohol polyoxyethylene ether and coconut acid diethanolamide.

[0037] In an optional embodiment, the pH adjuster is selected from one or more of citric acid, sodium citrate, sodium lactate, malic acid, lactic acid, tartaric acid, phosphoric acid, and triethanolamine.

[0038] In an optional embodiment, the preservative is selected from one or more of benzyl alcohol, benzoic acid, methyl paraben and 2,4-hexadienoic acid.

[0039] In an optional embodiment, the ingredients of the redness-repairing and soothing composition include, by weight: 0.1-10 parts of phenolic compounds, 0.5-10 parts of phenolic acid compounds, 0.1-6 parts of glycoside compounds, 1-25 parts of terpenoid compounds, 0.05-20 parts of amino acid compounds, 0.1-10 parts of fatty acids or their ester compounds, 1-20 parts of surfactants, 1-20 parts of organic solvents, 0.1-5 parts of stabilizers, 5-10 parts of alcohol-soluble proteins, and 20-500 parts of solvents.

[0040] On the basis of the above ratios, the inventors further optimized the ratios of the ingredients of the Xiuhong Soothing Composition. Measured by weight, the ingredients of the Xiuhong Soothing Composition include: 0.1-10 parts of phenolic compounds, 0.5-10 parts of phenolic acid compounds, 0.1-6 parts of glycoside compounds, 10-25 parts of terpenoid compounds, 5-15 parts of amino acid compounds, 1-8 parts of fatty acids or their ester compounds, 5-20 parts of surfactants, 1-20 parts of organic solvents, 0.5-2 parts of stabilizers, 5-10 parts of alcohol-soluble proteins, and 20-500 parts of water.

[0041] In a second aspect, the present invention also provides a method for preparing the above-mentioned redness-removing and soothing composition, which comprises uniformly mixing the redness-removing and soothing efficacy agent, glycoside compounds, amino acid compounds, fatty acids or their ester compounds, alcohol-soluble proteins, cosmetic excipients and solvents according to a proportion to obtain the redness-removing and soothing composition.

[0042] In an optional embodiment, the preparation method comprises:

[0043] The phenolic compound, the glycoside compound and the amino acid compound are dissolved in water and mixed to obtain a mixture I;

[0044] The phenolic acid compound, the terpenoid compound, the fatty acid or its ester compound and the organic solvent are mixed, heated, kept warm and stirred, and then cooled to room temperature to obtain a mixture II;

[0045] Then, the mixture II is added to the mixture I, and the mixture III is obtained after mixing; then, the alcohol-soluble protein and the stabilizer are added to the mixture III, and the mixture IV is obtained after mixing;

[0046] Add the surfactant to the mixture IV, mix well, add the preservative and the pH adjuster, mix well again to obtain the redness-removing and soothing composition.

[0047] In an optional embodiment, the heating temperature during the preparation of mixture II is 30-80°C; the speed of the heat-insulating stirring is 5000-10000 rpm, and the time is 1-30 min.

[0048] In an alternative embodiment, the pH of the redness-reducing and soothing composition is 6-8.

[0049] In a third aspect, the present invention also provides the use of the above redness-removing and soothing composition in the preparation of redness-removing and soothing products, and the above products include cosmetics and medicines for improving or treating skin redness and inflammation.

[0050] In an optional embodiment, the dosage form of the above product is any one of ointment, paste, lotion, cream, gel, solution, suspension, emulsion, patch or spray.

[0051] The present invention has the following beneficial effects:

[0052] The present invention combines phenolic compounds (such as hydroxytyrosol), phenolic acid compounds (such as caffeic acid and protocatechuic acid), glycoside compounds (such as gardenia glycoside) and terpenoid compounds (such as squalane) through strict screening to obtain an agent with synergistic redness-removing and soothing effects, and then adds other active ingredients on the basis of the agent to prepare a redness-removing and soothing composition. The components in the composition cooperate with each other synergistically to inhibit the release of inflammatory factors, relieve itching, resist inflammation, calm the skin, reduce vascular expansion, and at the same time enhance the skin's ability to lock in water, improve the skin's dehydration condition, and restore the skin to moisture and tenderness. DETAILED DESCRIPTION

[0053] To make the purpose, technical scheme and advantages of the embodiment of the present invention clearer, the technical scheme in the embodiment of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiment, it is carried out according to the conditions recommended by normal conditions or manufacturers. If the manufacturer is not specified in the reagents or instruments used, they are all conventional products that can be purchased commercially. Specifically, in the following experiments, the hydroxytyrosol, caffeic acid, protocatechuic acid, cocoyl diethanolamide, eicosapentaenoic acid, gardenia glycosides, cocoyl glucoside, squalane, squalene, histidine, cystine, myristic acid, mannitol, Brunei gum, wheat alcohol-soluble protein, zein, ethanol, and glycerol content used are all greater than or equal to 99%, and the olive leaf extract content is greater than or equal to 98%.

[0054] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0055] Example 1

[0056] A redness-repairing and soothing composition, comprising the following components (A), (B), (C), (D), (F), (G), (H), (I), (J), and (K) in parts by weight:

[0057] (A) is hydroxytyrosol, 5 parts by weight;

[0058] (B) is caffeic acid, 5 parts by weight;

[0059] (C) is geniposide, 3 parts by weight;

[0060] (D) is coconut oil diethanolamide, weight parts are 10 parts;

[0061] (F) is squalane, 15 parts by weight;

[0062] (G) is histidine, with a weight ratio of 15 parts;

[0063] (H) is myristic acid, 5 parts by weight;

[0064] (I) is 5 parts by weight of mannitol and 10 parts by weight of ethanol;

[0065] (J) is Brunei rubber, 0.5 parts by weight;

[0066] (K) is wheat prolamin, with a weight portion of 5 parts.

[0067] Furthermore, the above composition also includes water in an amount of 60 parts by weight.

[0068] A method for preparing a redness-repairing and soothing composition comprises the following steps:

[0069] (1) Add components (A), (C) and (G) into water and stir at 1000 rpm to obtain mixture I;

[0070] (2) Mix (B), (F), (H) and (I), heat to 50°C, stir at 5000 rpm for 5 min, and cool to room temperature to obtain mixture II;

[0071] (3) adding mixture II to mixture I and stirring to obtain mixture III;

[0072] (4) adding (K) and (J) to mixture III in sequence and stirring to obtain mixture IV;

[0073] (5) Component (D) was added to mixture III, and stirred at 8000 rpm for 10 min. Then, a preservative was added (in this embodiment, the amount of preservative added was 0.1 part), and the mixture was stirred. The pH was adjusted to 7. High-pressure homogenization was performed at 80 MPa for 15 min to obtain a composition.

[0074] Example 2

[0075] A redness-repairing and soothing composition, comprising the following components (A), (B), (C), (D), (F), (G), (H), (I), (J), and (K) in parts by weight:

[0076] (A) is hydroxytyrosol, 7 parts by weight;

[0077] (B) is caffeic acid, 2 parts by weight;

[0078] (C) is geniposide, with a weight portion of 1 part;

[0079] (D) is coconut oil diethanolamide, which has a weight ratio of 20 parts;

[0080] (F) is squalane, 5 parts by weight;

[0081] (G) histidine, 5 parts by weight;

[0082] (H) is myristic acid, 5 parts by weight;

[0083] (I) is mannitol and ethanol, the mannitol weight parts are 5 parts, and the ethanol weight parts are 5 parts;

[0084] (J) is Brunei rubber, 1 part by weight;

[0085] (K) is wheat prolamin, with a weight portion of 6 parts.

[0086] Furthermore, the above composition also includes water in an amount of 100 parts by weight.

[0087] The preparation method of a redness-repairing and soothing composition is the same as that of Example 1.

[0088] The other implementation methods and conditions in this embodiment are the same as those in Embodiment 1.

[0089] Example 3

[0090] A redness-repairing and soothing composition, comprising the following components (A), (B), (C), (D), (F), (G), (H), (I), (J), and (K) in parts by weight:

[0091] (A) is hydroxytyrosol, which has a weight portion of 0.5 parts;

[0092] (B) is caffeic acid, 8 parts by weight;

[0093] (C) is geniposide, with a weight ratio of 6 parts;

[0094] (D) is coconut oil diethanolamide, which has a weight ratio of 20 parts;

[0095] (F) is squalane, with a weight ratio of 25 parts;

[0096] (G) histidine, 20 parts by weight;

[0097] (H) is myristic acid, 8 parts by weight;

[0098] (I) is 5 parts by weight of mannitol and 15 parts by weight of ethanol;

[0099] (J) is Brunei rubber, 2 parts by weight;

[0100] (K) is wheat prolamin, with a weight portion of 8 parts.

[0101] Furthermore, the above composition also includes water in an amount of 80 parts by weight.

[0102] The preparation method of a redness-repairing and soothing composition is the same as that of Example 1.

[0103] The other implementation methods and conditions in this embodiment are the same as those in Embodiment 1.

[0104] Example 4

[0105] A redness-repairing and soothing composition, comprising the following components (A), (B), (C), (D), (F), (G), (H), (I), (J), and (K) in parts by weight:

[0106] (A) is tyrosol, 5 parts by weight;

[0107] (B) is protocatechuic acid, 5 parts by weight;

[0108] (C) is cocoyl glucoside, 3 parts by weight;

[0109] (D) is coconut oil diethanolamide, weight parts are 10 parts;

[0110] (F) is squalane, 15 parts by weight;

[0111] (G) is cystine, 15 parts by weight;

[0112] (H) is myristic acid, 5 parts by weight;

[0113] (I) is 5 parts by weight of mannitol and 10 parts by weight of ethanol;

[0114] (J) is Brunei rubber, 0.5 parts by weight;

[0115] (K) is wheat prolamin, with a weight portion of 5 parts.

[0116] Furthermore, the above composition also includes water in an amount of 60 parts by weight.

[0117] The preparation method of a redness-repairing and soothing composition is the same as that of Example 1.

[0118] The other implementation methods and conditions in this embodiment are the same as those in Embodiment 1.

[0119] Example 5

[0120] A redness-repairing and soothing composition, comprising the following components (A), (B), (C), (D), (F), (G), (H), (I), (J), and (K) in parts by weight:

[0121] (A) is hydroxytyrosol, 5 parts by weight;

[0122] (B) is caffeic acid, 5 parts by weight;

[0123] (C) is geniposide, 3 parts by weight;

[0124] (D) is coconut oil diethanolamide, weight parts are 10 parts;

[0125] (F) is squalane, 15 parts by weight;

[0126] (G) is cystine, 15 parts by weight;

[0127] (H) is eicosapentaenoic acid, 5 parts by weight;

[0128] (I) is glycerol, 15 parts by weight;

[0129] (J) is Brunei rubber, 0.5 parts by weight;

[0130] (K) is zein, with a weight portion of 5 parts.

[0131] Furthermore, the above composition also includes water in an amount of 60 parts by weight.

[0132] The preparation method of a redness-repairing and soothing composition is the same as that of Example 1.

[0133] The other implementation methods and conditions in this embodiment are the same as those in Embodiment 1.

[0134] Example 6

[0135] A redness-repairing and soothing composition, comprising the following components (A), (B), (C), (D), (F), (G), (H), (I), (J), and (K) in parts by weight:

[0136] (A) is hydroxytyrosol, 5 parts by weight;

[0137] (B) is 3 parts by weight of caffeic acid; 2 parts by weight of protocatechuic acid;

[0138] (C) is geniposide, 3 parts by weight;

[0139] (D) is coconut oil diethanolamide, weight parts are 10 parts;

[0140] (F) is squalane, 15 parts by weight;

[0141] (G) is cystine, 15 parts by weight;

[0142] (H) is 1 part by weight of eicosapentaenoic acid and 4 parts by weight of linolenic acid;

[0143] (I) is glycerol, 15 parts by weight;

[0144] (J) is Brunei rubber, 0.5 parts by weight;

[0145] (K) is zein, with a weight portion of 5 parts.

[0146] Furthermore, the above composition also includes water in an amount of 60 parts by weight.

[0147] The preparation method of a redness-repairing and soothing composition is the same as that of Example 1.

[0148] The other implementation methods and conditions in this embodiment are the same as those in Embodiment 1.

[0149] Comparative Example 1

[0150] In this comparative example, a redness-removing and soothing composition does not contain hydroxytyrosol and caffeic acid, and other components are the same as those in Example 1.

[0151] The preparation method of a redness-repairing and soothing composition is the same as that of Example 1.

[0152] The other implementation methods and conditions in this embodiment are the same as those in Embodiment 1.

[0153] Comparative Example 2

[0154] In this comparative example, a redness-repairing and soothing composition does not contain caffeic acid and gardenia glycoside, and the other components are the same as those in Example 1.

[0155] The preparation method of a redness-repairing and soothing composition is the same as that of Example 1.

[0156] The other implementation methods and conditions in this embodiment are the same as those in Embodiment 1.

[0157] Comparative Example 3

[0158] In this comparative example, a redness-repairing and soothing composition does not contain mannitol, Brunei gum, and wheat prolamin, and the other components are the same as those in Example 1.

[0159] The preparation method of a redness-repairing and soothing composition is the same as that of Example 1.

[0160] The other implementation methods and conditions in this embodiment are the same as those in Embodiment 1.

[0161] Comparative Example 4

[0162] In this comparative example, squalane and gardenia glycoside are not contained, and other implementation methods and conditions in this example are the same as those in Example 1.

[0163] Comparative Example 5

[0164] In this comparative example, only olive leaf extract and water are contained, wherein the mass fraction of olive leaf extract is consistent with the mass fraction of hydroxytyrosol in Example 1. Other embodiments of this example are the same as those in Example 1.

[0165] The ingredients of Examples 1-6 and Comparative Examples 1-5 are listed as shown in Table 1:

[0166] Table 1 Comparison of ingredients of Examples 1-6 and Comparative Examples 1-5

[0167]

[0168] Experimental example

[0169] Performance measurement

[0170] 1. Stability test

[0171] The stability of the products obtained in the above examples 1-6 and comparative examples 1-5 was tested, wherein the stability test was as follows: the products obtained in the above examples 1-6 and comparative examples 1-5 were placed at high temperature (50° C.) and low temperature (15° C.) for 24 hours, then returned to room temperature, and the morphology of the products was observed;

[0172] After high temperature and low temperature tests, the samples were restored to room temperature. There was no oil-water separation in Examples 1-6 and Comparative Examples 1-2, 4-5. Oil-water separation occurred in Comparative Example 3. This indicates that the product prepared by the composition of the present invention has good stability.

[0173] After the products obtained in Examples 1-6 were placed at room temperature for 6 months, the products in Examples 1-6 were still stable uniform emulsions without oil-water separation.

[0174] 2. Safety test

[0175] With reference to the human skin closed patch test method of the "Technical Specification for Safety of Cosmetics" (2015 edition), the samples in Examples 1 to 6 were taken for human patch tests, with 10 volunteers / group. None of the testers showed skin redness or allergic phenomena such as erythema, papules, and edema, which shows that the compositions and the prepared emulsions in the present invention are safe and do not cause any adverse phenomena.

[0176] 3. Inflammatory factor test

[0177] (1) Mouse mononuclear macrophage RAW 264.7 cell line was cultured in DMEM high glucose medium containing 10% FBS at 37°C and 5% CO2 in a constant temperature incubator. The cells grew logarithmically, and the medium was replaced every 24 hours. The cells were passaged when 80% of the cells were grown. After the cells reached the third generation and were stable, they were used for subsequent experiments.

[0178] (2) Test sample preparation:

[0179] A test sample is prepared using a cell culture medium, wherein:

[0180] Experimental group: The composition of Example 1 was prepared with DMEM culture medium into test samples with volume fractions of 1.0%, 2.0%, 5.0%, 10.0%, and 20.0%.

[0181] (3) Detection of RAW 264.7 cell viability by CCK8 assay

[0182] Effect of the composition of test example 1 on the activity of mouse mononuclear macrophage RAW 264.7 cells: macrophages in the logarithmic growth phase were taken and scraped, and pipetted until they were dispersed into single cells. Cell counts were taken, and a certain number of cells were added to the corresponding culture medium at 4×10 4The cells were inoculated into a 96-well plate at a density of 100 μL / mL, and cultured for 24 h.

[0183] The culture medium on the 96-well plate was discarded, and 10 μL of the test sample of different concentrations was added to the culture plate. (The experimental group was added with the culture medium containing the composition of Example 1 of different concentrations, and the control group was added with the culture medium without the composition of Example 1). The culture plate was cultured in an incubator (37° C., 5% CO2) for 24 hours. There were 5 replicate wells in each experimental group and control group.

[0184] 10 μL CCK8 reagent was added, and the cells were cultured in a 37°C, 5% CO2 cell culture incubator for 40 min. The absorbance (OD value) at 450 nm was measured using an ELISA reader. The cell survival rate of each group was calculated as follows: cell survival rate (%) = [(As-Ab) / (Ac-Ab)] × 100%.

[0185] As: absorbance of the experimental group (containing cells, culture medium, CCK-8 solution and the composition of Example 1);

[0186] Ac: absorbance of the control group (containing cells, culture medium, CCK-8 solution, but not containing the composition of Example 1); Ab: absorbance of the blank group (containing culture medium, CCK-8 solution, but not containing cells or the composition of Example 1).

[0187] When the test sample contained 20% of the composition of Example 1 by volume, the cell survival rate was 81.52%, and the cell survival rates of other experimental groups were all greater than 85%. Therefore, when the addition amount of the composition of Example 1 of the present invention is less than or equal to 20%, it is a safe addition amount. Subsequent experiments selected the composition of Example 1 of the present invention to be added at 5%.

[0188] Similarly, after replacing the composition of Example 1 in the above experimental group with the compositions of Example 4 and Comparative Examples 1-5, tests were performed to calculate the cell survival rate of each group.

[0189] When the test sample contained less than or equal to 10% of Example 4 and Comparative Examples 1-5 by volume fraction, the relative cell activity of each group was higher than 85%, which was a safe addition amount. Subsequent experiments were conducted with Example 4 and Comparative Examples 1-5 with a composition addition amount of 5%.

[0190] (4) Nitrate reductase method to detect NO content

[0191] First, the compositions of Example 1, Example 4 and Comparative Examples 1-5 of the present invention were prepared into a test sample solution with a volume percentage of 5.0% using complete culture medium (MEM+10% FBS).

[0192] Take the cells from step (1) above and inoculate them in a 96-well plate at a density of 4×10 4 / mL, incubate for 24h.

[0193] The culture plate was taken out, the original culture medium in the wells was discarded, the above test samples were added, and cultured for 1 hour.

[0194] Then, LPS (final concentration of 1 μg / mL) was added to the experimental group and the model group, and complete culture medium was added to the blank group. The culture was continued for 24 h, and the supernatant was collected. The specific steps were carried out according to the instructions of the NO kit.

[0195] The absorbance was measured at 540 nm, and the NO content and inhibition rate were calculated.

[0196] Calculation formula: NO content [c / (μmol·L 1 )] = (average OD value of the assay tube × average OD value of the blank tube) / (average OD value of the standard tube × average OD value of the blank tube) × concentration of the standard × dilution factor of the sample.

[0197] NO inhibition rate (%) = (average NO content in model group × average NO content in experimental group) / average NO content in model group × 100%.

[0198] Table 2 Effects of different groups on LPS-induced NO release from macrophages

[0199]

[0200] As shown in Table 2, the composition of the present invention can significantly inhibit the inflammatory factor NO, making the NO content close to the normal level (blank group). The compositions in Comparative Examples 1-5 also have a certain inhibitory effect on the inflammatory factor NO, but the effect is significantly weaker than that of Example 1 and Examples 4-6.

[0201] (5) ELISA method to detect IL-6 content

[0202] First, the compositions of Example 1, Example 4 and Comparative Examples 1-5 of the present invention were prepared into a test sample solution with a volume percentage of 5.0% using complete culture medium (MEM+10% FBS).

[0203] Take the cells from step (1) above and add 4×10 5 The cells were seeded in 24-well plates at a density of 100 / mL and incubated for 24 h.

[0204] The culture plate was taken out, the original culture medium in the wells was discarded, and the above test samples were added and cultured for 1 hour.

[0205] Then, LPS (final concentration of 1 μg / mL) was added to the experimental group and the model control group, and complete medium was added to the blank group. The cells were cultured for 20 h, and the cell supernatant was collected. The specific steps were operated according to the instructions of the ELISA kit, and the absorbance OD value was measured at 450 nm. The standard curve was drawn to calculate the IL 6 content and inhibition rate. IL 6 inhibition rate (%) = (average IL 6 content of model control group IL 6 average content of experimental group IL 6) / average IL 6 content of model control group × 100%.

[0206] Table 3 Effects of different groups on LPS-induced macrophage release of IL-6

[0207]

[0208] As shown in Table 3, the composition of the present invention can significantly inhibit the inflammatory factor IL 6. The comparison of the experimental group shows that when the composition of Example 1 of the present invention is added at 5%, the content of the inflammatory factor IL 6 is relatively low, indicating that the composition of Example 1 has a better anti-inflammatory effect.

[0209] 4. Skin irritation repair effect test:

[0210] 1) 110 volunteers (aged 20-55 years old) who were sensitive to capsaicin were selected and randomly divided into 11 groups, with 10 people in each group. The inner side of the left arm of the volunteers was used as the test site, and a square area of ​​2 cm × 2 cm was selected as the test area. There were no skin diseases in the test area.

[0211] Specifically, the first group of 10 people smeared the sample in Example 1, the second group of 10 people smeared the sample in Example 2, the third group of 10 people smeared the sample in Example 3, the fourth group of 10 people smeared the sample in Example 4, the fifth group of 10 people smeared the sample in Example 5, the sixth group of 10 people smeared the sample in Example 6, the seventh group of 10 people smeared the sample in Comparative Example 1, the eighth group of 10 people smeared the sample in Comparative Example 2, and the ninth group of 10 people smeared the sample in Comparative Example 3, and the average value of each group of test results was taken. The tenth group of 10 people smeared the sample in Comparative Example 4, and the average value of each group of test results was taken. The eleventh group of 10 people smeared the sample in Comparative Example 5, and the average value of each group of test results was taken.

[0212] 2) Wash the test area with clean water, wait for 10 minutes, and then use the German CK multifunctional skin tester to measure the skin redness value as T0, and test three times in a row to take the average value; then stimulate the test area with a 50% mass fraction capsaicin aqueous solution (about 0.01mL), and continue to stimulate for 15 minutes before collecting data;

[0213] 3) Apply the samples prepared in Examples 1-6 and Comparative Examples 1-5 to the test area, collect data after 10 minutes and 30 minutes, test three times in a row, take the average value, count the values ​​measured each time on the test area of ​​the volunteer, and calculate the change in redness value. The greater the change in redness value, the greater the degree of skin redness, and the worse the redness repairing and soothing effect of the sample.

[0214] Skin redness value change rate (%) = (T T0) / T0×100%.

[0215] The skin irritation repair effects of the samples prepared in Examples 1-6 and Comparative Examples 1-5 are shown in Table 4.

[0216] Table 4: Effect of samples on skin irritation and repair

[0217]

[0218]

[0219] As can be seen from Table 4, the samples in Examples 1-6 all have a certain degree of repair effect on the skin after stimulation by the capsaicin aqueous solution. Among them, the product in Example 1 has the best skin repair effect. After 30 minutes of application, the redness value change rate is 0.6%, and the skin almost returns to normal. This shows that the components in Example 1 work synergistically, can better play the redness repairing and soothing effect, and quickly repair damaged skin.

[0220] Relatively speaking, there is no hydroxytyrosol and caffeic acid component in Comparative Example 1, and the skin repair effect is poor; there is a lack of caffeic acid and gardenia glycoside components in Comparative Example 2, and the repair effect is not good; the sample in Comparative Example 3 has a certain repair effect, but lacks mannitol, ethanol, Brunei gum, and wheat alcohol-soluble protein components, and the active ingredients such as hydroxytyrosol and caffeic acid are not protected by wheat alcohol-soluble protein, and due to the lack of mannitol, ethanol, etc., the active ingredients cannot be evenly dispersed, and a stable emulsion cannot be formed. The active ingredients such as hydroxytyrosol and caffeic acid are easily oxidized and ineffective, so the effect is not significantly improved. In Comparative Example 4, squalane and gardenia glycoside are lacking, and the components cannot work well together, and the effect is not good; because squalane can promote the transdermal absorption of active ingredients (hydroxytyrosol, caffeic acid, amino acids), the effective ingredients are fully absorbed and play a role, and gardenia glycoside can promote the mutual dissolution between oily components and water-soluble components, and mix evenly. In Comparative Example 5, the olive leaf extract has a certain redness-repairing effect, but lacks the compatibility of other components, so the effect of the sample prepared in Comparative Example 5 is inferior to that of the sample in the embodiment of the present invention.

[0221] 5. Sensitive skin test

[0222] 70 volunteers with sensitive skin (thin skin stratum corneum, many subcutaneous red blood streaks, sensitive to cold and heat stimuli) were recruited and randomly divided into 7 groups, with 10 people in each group.

[0223] Sensitive skin volunteers used typical representative examples 1 and 4 of the present invention and samples of comparative examples 1-5 to test. After facial cleansing, the samples were applied to the face twice a day. After continuous use for 3 months, scores were given based on the actual effects. When the scores were finally counted, the average value was taken, and the score results are shown in Table 5 below.

[0224] Table 4 Scoring criteria

[0225]

[0226] Table 5 Volunteers' experience rating results

[0227]

[0228] As shown in Table 5, after three months, the volunteers who used the samples in Examples 1 and 4 had significantly relieved subcutaneous capillary dilation, thickened stratum corneum, significantly improved redness, and significantly reduced skin sensitivity. The volunteers who used the samples in Comparative Examples 1 and 2 had slightly improved skin sensitivity after three months, and the volunteers who used the samples in Comparative Examples 3-5 had slightly improved skin sensitivity after three months. After comprehensive comparison, it is shown that only the synergistic effect of the components of the composition of the present invention can have a better redness repair and soothing effect, enhance skin toughness, enhance skin barrier, and better resist external stimuli.

[0229] 6. Skin moisture and elasticity test

[0230] For the 40 volunteers mentioned above, the skin moisture and elasticity before and after using the samples of Examples 1, 4 and Comparative Examples 1-2 were tested using an intelligent skin detector, model MLB D0028. The skin moisture increase rate and elasticity increase rate were calculated and the average values ​​were taken. The results are shown in Table 6.

[0231] Table 6 Results of skin moisture increase rate and elasticity increase rate

[0232]

[0233] As shown in Table 6, the samples in Examples 1 and 4 can effectively increase the moisture content of the skin and improve the elasticity of the skin. The samples in Comparative Examples 1-2 have no obvious effect. This shows that the samples prepared by the composition of the present invention have a moisturizing effect, can increase the moisture content of the skin, lock in moisture, prevent moisture loss, and increase the elasticity of the skin.

[0234] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A redness-repairing and soothing composition, characterized in that: Includes redness-removing and soothing agents; The redness-repairing and soothing agent comprises phenolic compounds and phenolic acid compounds in a mass ratio of (0.1-10):(0.5-10); The phenolic compound is selected from at least one of hydroxytyrosol and tyrosol; The phenolic acid compound is selected from at least one of caffeic acid, protocatechuic acid, plateau catechuic acid and their ester derivatives.

2. The redness-removing and soothing composition according to claim 1, characterized in that: The mass ratio of the phenolic compound to the phenolic acid compound in the redness-repairing and soothing agent is (0.1-10):(1-10); Preferably, the mass ratio of phenolic compounds to phenolic acid compounds in the redness-repairing and soothing agent is (0.1-5):

1.

3. The redness-removing and soothing composition according to claim 2, characterized in that: The ingredients of the redness-repairing and soothing agent also include terpenoid compounds; Preferably, the terpenoid compound is selected from at least one of squalane and squalene; Preferably, the mass ratio of the terpenoid compound to the phenolic compound is (1-25):(0.1-10); Preferably, the mass ratio of the terpenoid compound to the phenolic compound is (10-25):(0.1-10).

4. The redness-removing and soothing composition according to claim 3, characterized in that: The ingredients of the redness-repairing and soothing agent also include glycoside compounds; Preferably, the glycoside compound is selected from at least one of gardenia glycoside and cocoyl glucoside; Preferably, the mass ratio of the glycoside compound to the phenolic compound is (0.1-6):(0.1-10).

5. The redness-removing and soothing composition according to claim 4, characterized in that: The redness-repairing and soothing agent is selected from one or more of hydroxytyrosol, caffeic acid, protocatechuic acid, squalane, squalene, gardenia glycoside and coconut glucoside; Preferably, the ingredients of the redness-repairing and soothing agent are hydroxytyrosol, caffeic acid, squalane and gardenia glycoside; Preferably, the ingredients of the redness-repairing and soothing agent are hydroxytyrosol, protocatechuic acid, squalane and gardenia glycoside; Preferably, the redness-removing and soothing agent comprises hydroxytyrosol, caffeic acid, protocatechuic acid, squalane and gardenia glycoside.

6. The redness-removing and soothing composition according to claim 5, characterized in that: The ingredients of the redness-repairing and soothing composition also include amino acid compounds, fatty acids or their ester compounds, alcohol-soluble proteins, cosmetic excipients and solvents; Preferably, the amino acid compound is selected from at least one of glycine, histidine and cystine; Preferably, the fatty acid or its ester compound is at least one selected from myristic acid, palmitic acid, oleic acid, linolenic acid, linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid; Preferably, the alcohol-soluble protein is selected from at least one of wheat alcohol-soluble protein and zein. Preferably, the cosmetic excipients include surfactants, stabilizers, and pH adjusters; Preferably, the surfactant is selected from at least one of lauryl alcohol polyoxyethylene ether and coconut acid diethanolamide; Preferably, the stabilizer is selected from at least one of Brunei gum and glucomannan; Preferably, the pH regulator is selected from one or more of citric acid, sodium citrate, sodium lactate, malic acid, lactic acid, tartaric acid, phosphoric acid, and triethanolamine; Preferably, the solvent comprises an organic solvent and water; Preferably, the organic solvent is selected from at least one of mannitol, glycerol and ethanol.

7. The redness-removing and soothing composition according to claim 6, characterized in that: The ingredients of the redness-repairing and soothing composition include, by weight: 0.1-10 parts of phenolic compounds, 0.5-10 parts of phenolic acid compounds, 0.1-6 parts of glycoside compounds, 1-25 parts of terpenoid compounds, 0.05-20 parts of amino acid compounds, 0.1-10 parts of fatty acids or their ester compounds, 1-20 parts of surfactants, 5-10 parts of alcohol-soluble proteins, 1-20 parts of organic solvents, 0.1-5 parts of stabilizers, and 20-500 parts of water.

8. The redness-removing and soothing composition according to claim 7, characterized in that: The ingredients of the redness-repairing and soothing composition include, by weight: 0.1-10 parts of phenolic compounds, 0.5-10 parts of phenolic acid compounds, 0.1-6 parts of glycoside compounds, 10-25 parts of terpenoid compounds, 5-15 parts of amino acid compounds, 1-8 parts of fatty acids or their ester compounds, 5-20 parts of surfactants, 5-10 parts of alcohol-soluble proteins, 1-20 parts of organic solvents, 0.5-2 parts of stabilizers, and 20-500 parts of water.

9. The method for preparing the redness-removing and soothing composition according to any one of claims 1 to 8, characterized in that: The redness-removing and soothing agent, glycoside compounds, amino acid compounds, fatty acids or ester compounds thereof, alcohol-soluble proteins, cosmetic excipients and solvents are mixed uniformly according to a proportion to obtain the redness-removing and soothing composition; Preferably, the preparation method comprises: The phenolic compound, the glycoside compound and the amino acid compound are dissolved in water and mixed to obtain a mixture I; The phenolic acid compound, the terpenoid compound, the fatty acid or its ester compound and the organic solvent are mixed, heated, kept warm and stirred, and then cooled to room temperature to obtain a mixture II; Then, the mixture II is added to the mixture I, and the mixture III is obtained after mixing; then, the alcohol-soluble protein and the stabilizer are added to the mixture III, and the mixture IV is obtained after mixing; Adding a surfactant to the mixture IV, mixing well, adding a preservative and a pH adjuster, and mixing well again to obtain the redness-removing and soothing composition; Preferably, the heating temperature during the preparation of the mixture II is 30-80°C; the speed of the heat preservation and stirring is 5000-10000 rpm, and the time is 1-30 min; Preferably, the pH of the redness-removing and soothing composition is 6-8.

10. Use of the redness-removing and soothing composition according to any one of claims 1 to 8 in preparing a product for skin, characterized in that: The products include cosmetics and medicines that improve or treat skin redness and inflammation; Preferably, the dosage form of the product is any one of ointment, paste, lotion, cream, gel, solution, suspension, emulsion, patch or spray.

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