Process and method for extracting chlorogenic acid from honeysuckle
By designing an extraction equipment including a mixing barrel, a crushing barrel and a transmission device, the problem of incomplete crushing of existing equipment is solved, and efficient crushing of honeysuckle and effective extraction of chlorogenic acid are achieved.
Patent Information
- Application Number
- CN202411571301.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-04
- Publication Date
- 2025-02-14
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The crushing device of the existing extraction equipment is too simple to effectively crush honeysuckle, resulting in insufficiency of extraction.
An extraction equipment including a mixing barrel, a crushing barrel and a transmission device was designed. The rotating rod and agitating leaves were driven by a motor to mechanically crush the honeysuckle, and combined with frozen crushing and ultrasonic treatment, further improving the crushing effect.
It significantly improves the crushing effect of honeysuckle, improves the extraction efficiency of chlorogenic acid, and makes the extraction process more efficient and reliable.
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Figure CN119425149A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of plant extraction equipment, and in particular to an extraction process and method for extracting chlorogenic acid from honeysuckle. Background Art
[0002] With the continuous development of society, honeysuckle is a general term for Chinese medicinal materials and plants. Honeysuckle, also known as honeysuckle, is a perennial semi-evergreen twining woody vine of the Caprifoliaceae family. The chlorogenic acid contained in honeysuckle has a good effect on preventing and treating upper respiratory tract infections and gastrointestinal diseases, and has a good effect on eliminating free radicals in the human body, delaying aging, and improving human immune function.
[0003] For example, Publication No. 202022753165.X discloses an efficient extraction device for chlorogenic acid from honeysuckle stems and leaves, including an extraction device body, the extraction device body including a fixed shell, support legs, a bin door and a feed port, the bottom of the side of the fixed shell is fixedly connected to the support legs, the front surface of the fixed shell is fixedly connected to the bin door, the upper end of the fixed shell is provided with a feed port near the left side, and the middle position of the upper end of the fixed shell is fixedly connected to an adjustment device, the adjustment device includes a motor, a stirring rod, a bin, a fixed seat, a battery, a heating resistor, a catalyst box, a water pipe, a support block, a first spring, a baffle, a first fixed plate and a fixed tube. The present invention is provided with an adjustment device, which effectively avoids the phenomenon of excessive artificial addition of leaching solution. The device is provided with a distinguishing and collecting device so that the residue remains on the upper end of the second fixed plate, which is convenient for operators to clean and maintain.
[0004] In the above patent, the residue is left on the upper end of the second fixed plate through the adjusting device and the collecting device, which is convenient for the operator to clean and maintain. However, the extraction equipment cannot efficiently crush the honeysuckle due to the overly simple crushing device. Summary of the invention
[0005] The present invention provides an extraction process and method for extracting chlorogenic acid from honeysuckle, which solves the problem that the extraction equipment in the related art cannot efficiently crush the honeysuckle due to the overly simple crushing device.
[0006] The technical solution of the present invention is as follows:
[0007] A process and method for extracting chlorogenic acid from honeysuckle, comprising an extraction device for extracting chlorogenic acid from honeysuckle, the extraction device also comprising a stirring barrel, a lid is arranged above the stirring barrel, a stirrer and a transmission device are arranged above the lid, a crushing barrel is arranged below the lid, and a discharge pipe is arranged below the stirring barrel;
[0008] The mixing barrel comprises a barrel body, a limiting rod and a groove one. The bottom of the barrel body is arranged in an arc-shaped structure. The limiting rod is fixedly connected to the inner wall of the upper side of the barrel body. The limiting rod is provided with a groove one.
[0009] The present invention further stores the crushed and filtered honeysuckle juice.
[0010] Preferably, the lid includes a cover plate, a liquid inlet pipe, a fixing card and a second groove, the cover plate is engaged with the barrel mouth of the barrel body, the second groove is provided at the bottom of the cover plate, the liquid inlet pipe is fixedly connected to the top of the cover plate, and the liquid inlet pipe is connected to the inner cavity of the barrel body.
[0011] The present invention further seals the barrel body and limits and fixes the crushing barrel.
[0012] Preferably, a fixing card is symmetrically fixedly connected to the upper surface of the cover plate, and a second gear is rotatably connected in the middle of one side of the fixing card, and the second gear passes through the cover plate.
[0013] Furthermore, the cover plate limits and fixes the gear 2.
[0014] Preferably, the transmission device includes gear 1, a rotating shaft and bevel gear 2, the rotating shaft is rotatably connected to the other side of the fixed card, one side of the rotating shaft is fixedly connected to gear 1, the gear 1 passes through the cover plate, and the other side of the rotating shaft is fixedly connected to bevel gear 2.
[0015] Furthermore, the present invention, the rotation of the second bevel gear drives the rotating shaft to rotate, and further drives the first gear to rotate.
[0016] Preferably, the agitator comprises a rotating rod, a stirring blade and a bevel gear 1, the upper side of the rotating rod is rotatably connected to the cover plate, the upper side of the rotating rod is fixedly connected to the bevel gear 1, and the bevel gear 1 is meshedly connected to the bevel gear 2.
[0017] Furthermore, the present invention, the rotation of the rotating rod drives the bevel gear 1 to rotate, and the rotation of the bevel gear 1 drives the bevel gear 2 to rotate.
[0018] Preferably, the upper end of the rotating rod is fixedly connected to the output end of the motor 2, the side of the motor 2 is fixedly connected to the cover plate, and a plurality of groups of stirring blades are fixedly connected at equal angles on the rotating rod.
[0019] The present invention further comprises that the second motor drives the rotating rod to rotate, and then drives the stirring blade to rotate, so as to grind the honeysuckle in the grinding barrel.
[0020] Preferably, the crushing barrel includes a filter barrel, an annular rack, an annular rod, groove three and a rotating wheel. The filter barrel is embedded in the inner cavity of the barrel body. The barrel mouth of the filter barrel is fixedly connected with an annular rack, and the annular rack is meshed with gear one and gear two.
[0021] The present invention further comprises gear 1 and gear 2 which limit and fix the filter barrel, and gear 1 rotates on the annular rack to drive the filter barrel to rotate in the opposite direction.
[0022] Preferably, the outer wall of the filter barrel is fixedly connected with an annular rod, and four grooves three are opened at equal angles on the annular rod. There are movable wheels in the grooves three. The two sides of the wheel are rotatably connected to the annular rod, and the lower side of the wheel is slidably connected to the groove one.
[0023] Furthermore, the present invention has a rotating wheel and a groove that are slidably connected to limit and fix the filter barrel while assisting the filter barrel to rotate.
[0024] Preferably, the discharge pipe includes a pipeline and an auger, the upper end of the pipeline is fixedly connected to the bottom of the barrel body, and the inner cavity of the pipeline is communicated with the inner cavity of the barrel body.
[0025] The present invention further provides that the pipeline discharges the juice produced by the crushed honeysuckle.
[0026] Preferably, an auger is movable in the pipeline, one end of the auger is rotatably connected to the pipeline wall, the edge of the auger is fit-connected to the inner wall of the pipeline, and one end of the auger passes through the pipeline and is fixedly connected to the output end of motor 1.
[0027] Furthermore, the motor drives the auger to rotate, so as to stably transport the produced juice.
[0028] A method for extracting chlorogenic acid from honeysuckle, comprising the following steps:
[0029] Step S1, crushing:
[0030] Step S11, mechanical crushing:
[0031] Step S111, pour honeysuckle into the filter barrel, embed the filter barrel into the barrel body, and drive the rotating wheel on the annular rod to move, so that the rotating wheel is slidably connected with the groove three, and the filter barrel is limited and fixed while assisting the rotation of the filter barrel;
[0032] Step S112, the second motor drives the rotating rod to rotate, the rotating rod drives the stirring blade to rotate, the honeysuckle in the filter barrel is stirred and crushed, the rotating rod drives the bevel gear 1 to rotate, the bevel gear 1 drives the bevel gear 2 to rotate, the bevel gear 2 drives the rotating shaft to rotate, the rotating shaft drives the gear 1 to rotate on the annular rack, the annular rack is fixedly connected to the barrel mouth of the filter barrel, the gear 1 drives the filter barrel to rotate in the opposite direction to the stirring blade, so as to crush the honeysuckle;
[0033] Step S12, freezing and crushing: fresh or dried honeysuckle material is placed in a sealed container made of stainless steel, and liquid nitrogen is passed through the container to freeze the material, so that the temperature of the honeysuckle material drops to -196°C and becomes a glass brittle state; a supersonic nitrogen gas flow is then passed through the sealed container, so that the low-temperature honeysuckle material is rapidly crushed under the impact and friction of the supersonic gas flow, and cell-level honeysuckle powder is obtained;
[0034] Step S2, enzyme treatment: add deionized water with a pH of 6.5 to the honeysuckle powder and stir evenly, soak for 1 to 2 hours, and the amount of deionized water added is 10 to 15 times the weight of the honeysuckle powder; then add exoglucanase to the soaking liquid and transfer it to a closed container, and then stir evenly;
[0035] Step S3, ultrasonic treatment: the mixture after enzyme treatment is subjected to ultrasonic treatment at room temperature and at an ultrasonic frequency of 60 to 100 KHz for 25 to 30 minutes;
[0036] Step S4, filtering: filtering the mixture after ultrasonic treatment with a high-speed centrifuge or filtering out the filtrate with a plate and frame filter press;
[0037] Step S5, extraction: the filtered residue is forced to reflux with deionized water 6 to 12 times the weight of the residue for 1 to 1.5 hours, and the extraction is repeated 3 to 6 times to obtain an extract;
[0038] Step S6, concentration: combining the filtrate of step S4 and the extract of step S5 to obtain a chlorogenic acid initial solution, concentrating the chlorogenic acid initial solution under a vacuum condition of 0.05 MPa, adding chitosan 3% times the weight of the concentrated solution to the concentrated solution, stirring, and flocculating; then filtering, and decolorizing the filtrate with activated carbon to obtain a decolorized concentrated solution;
[0039] Step S7, purification: the concentrated solution after decolorization is saturatedly adsorbed by a strong polar macroporous macroporous resin column, eluted with ethanol water after adsorption, and the eluate is collected, concentrated, and dried to obtain a chlorogenic acid extract;
[0040] Step S8, chlorogenic acid extract liquid output: inject water into the bucket through the liquid inlet pipe to drive the juice in the filter bucket to filter and flow, and the motor drives the auger to rotate in the pipeline to stably transport the juice;
[0041] Step S9, cleaning: open the cover plate, open the barrel body, and move the filter barrel to disassemble the filter barrel, so as to facilitate the cleaning of the waste residue in the filter barrel.
[0042] The working principle and beneficial effects of the present invention are:
[0043] 1. In the present invention, motor 2 drives the rotating rod to rotate, and the rotating rod drives the stirring blade to rotate, so that the honeysuckle in the filter barrel is stirred and crushed. The rotation of the rotating rod drives the bevel gear 1 to rotate, the bevel gear 1 drives the bevel gear 2 to rotate, the bevel gear 2 drives the rotating shaft to rotate, the rotating shaft drives the gear 1 to rotate on the annular rack, the annular rack is fixedly connected to the barrel mouth of the filter barrel, and the gear 1 drives the filter barrel to rotate in the opposite direction to the stirring blade, thereby greatly improving the crushing effect of the honeysuckle.
[0044] 2. In the present invention, the cover plate is opened, the barrel body is opened, and the filter barrel can be disassembled by moving the filter barrel, so that the waste residue in the filter barrel can be cleaned conveniently. BRIEF DESCRIPTION OF THE DRAWINGS
[0045] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0046] Figure 1 This is a front view of the three-dimensional structure of the present invention;
[0047] Figure 2 It is a cross-sectional view of the three-dimensional structure of the present invention;
[0048] Figure 3 For the present invention Figure 2 A is an enlarged view of the three-dimensional structure.
[0049] In the figure:
[0050] 1. Mixing barrel; 101. Barrel body; 102. Limit rod; 103. Groove 1;
[0051] 2. Cover; 201. Cover plate; 202. Liquid inlet pipe; 203. Fixing card; 204. Groove 2;
[0052] 3. Agitator; 301. Rotating rod; 302. Agitating blade; 303. Bevel gear 1;
[0053] 4. Transmission device; 401. Gear 1; 402. Rotating shaft; 403. Bevel gear 2;
[0054] 5. Gear 2;
[0055] 6. Discharge pipe; 601. Pipeline; 602. Auger;
[0056] 7. Motor 1; 8. Motor 2:
[0057] 9. Crushing barrel; 901. Filter barrel; 902. Ring rack; 903. Ring rod; 904. Groove three; 905. Rotating wheel. DETAILED DESCRIPTION
[0058] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0059] like Figure 1 and Figure 2 As shown, this embodiment proposes an extraction process and method for extracting chlorogenic acid from honeysuckle, including an extraction device for extracting chlorogenic acid from honeysuckle, the extraction device also includes a stirring barrel 1, a lid 2 is arranged above the stirring barrel 1, a stirrer 3 and a transmission device 4 are arranged above the lid 2, a crushing barrel 9 is arranged below the lid 2, and a discharge pipe 6 is arranged below the stirring barrel 1;
[0060] The mixing barrel 1 includes a barrel body 101, a limiting rod 102 and a groove 103. The bottom of the barrel body 101 is arranged in an arc-shaped structure. The limiting rod 102 is fixedly connected to the inner wall of the upper side of the barrel body 101. The limiting rod 102 is provided with a groove 103.
[0061] like Figure 1 and Figure 2 As shown, the lid 2 includes a cover plate 201, a liquid inlet pipe 202, a fixing card 203 and a second groove 204. The cover plate 201 is engaged with the barrel mouth of the barrel body 101, and a second groove 204 is provided at the bottom of the cover plate 201. The liquid inlet pipe 202 is fixedly connected to the top of the cover plate 201, and the liquid inlet pipe 202 is connected to the inner cavity of the barrel body 101.
[0062] like Figure 1 and Figure 2 As shown, a fixing card 203 is symmetrically fixedly connected to the cover plate 201 , and a gear 2 5 is rotatably connected to the middle of one side of the fixing card 203 , and the gear 2 5 passes through the cover plate 201 .
[0063] like Figure 2 As shown, the transmission device 4 includes a gear 1 401, a rotating shaft 402 and a bevel gear 2 403. The rotating shaft 402 is rotatably connected to the other side of the fixed card 203. One side of the rotating shaft 402 is fixedly connected to the gear 1 401. The gear 1 401 passes through the cover plate 201. The other side of the rotating shaft 402 is fixedly connected to the bevel gear 2 403.
[0064] like Figure 2 As shown, the stirrer 3 includes a rotating rod 301, a stirring blade 302 and a bevel gear 1 303. The upper side of the rotating rod 301 is rotatably connected to the cover plate 201, and the upper side of the rotating rod 301 is fixedly connected to the bevel gear 1 303. The bevel gear 1 303 is meshed with the bevel gear 2 403.
[0065] like Figure 2As shown, the upper end of the rotating rod 301 is fixedly connected to the output end of the motor 2 8 , the side of the motor 2 8 is fixedly connected to the cover plate 201 , and multiple groups of stirring blades 302 are fixedly connected at equal angles on the rotating rod 301 .
[0066] like Figure 2 As shown, the crushing barrel 9 includes a filter barrel 901, an annular rack 902, an annular rod 903, a groove three 904 and a rotating wheel 905. The filter barrel 901 is embedded in the inner cavity of the barrel body 101. The barrel mouth of the filter barrel 901 is fixedly connected with an annular rack 902, and the annular rack 902 is meshed with gear one 401 and gear two 5.
[0067] like Figure 2 and Figure 3 As shown, the outer wall of the filter barrel 901 is fixedly connected to a ring rod 903, and four grooves 904 are opened at equal angles on the ring rod 903. There are movable wheels 905 in the grooves 904. The two sides of the wheel 905 are rotatably connected to the ring rod 903, and the lower side of the wheel 905 is slidably connected to the groove 103.
[0068] like Figure 2 As shown, the discharge pipe 6 includes a pipe 601 and an auger 602 . The upper end of the pipe 601 is fixedly connected to the bottom of the barrel body 101 , and the inner cavity of the pipe 601 is communicated with the inner cavity of the barrel body 101 .
[0069] like Figure 2 As shown, an auger 602 moves inside the pipe 601, one end of the auger 602 is rotatably connected to the pipe wall of the pipe 601, the edge of the auger 602 is fitted and connected to the inner wall of the pipe 601, and one end of the auger 602 passes through the pipe 601 and is fixedly connected to the output end of the motor 7.
[0070] like Figure 1-3 As shown, the extraction method of the extraction device for extracting chlorogenic acid from honeysuckle is characterized by comprising the following steps:
[0071] Step S1, crushing:
[0072] Step S11, mechanical crushing:
[0073] Step S111, pour honeysuckle into the filter barrel 901, embed the filter barrel 901 into the barrel body 101, and the filter barrel 901 drives the rotating wheel 905 on the annular rod 903 to move, so that the rotating wheel 905 is slidably connected with the groove 3 904, and the filter barrel 901 is limited and fixed while assisting the rotation of the filter barrel 901;
[0074] Step S112, the motor 28 drives the rotating rod 301 to rotate, the rotating rod 301 drives the stirring blade 302 to rotate, and the honeysuckle in the filter barrel 901 is stirred and crushed, the rotating rod 301 drives the bevel gear 1 303 to rotate, the bevel gear 1 303 drives the bevel gear 2 403 to rotate, the bevel gear 2 403 drives the rotating shaft 402 to rotate, the rotating shaft 402 drives the gear 1 401 to rotate on the annular rack 902, the annular rack 902 is fixedly connected to the barrel mouth of the filter barrel 901, and the gear 1 401 drives the filter barrel 901 to rotate in the opposite direction to the stirring blade 302 to crush the honeysuckle;
[0075] Step S12, freezing and crushing: fresh or dried honeysuckle material is placed in a sealed container made of stainless steel, and liquid nitrogen is passed through the container to freeze the material, so that the temperature of the honeysuckle material drops to -196°C and becomes a glass brittle state; a supersonic nitrogen gas flow is then passed through the sealed container, so that the low-temperature honeysuckle material is rapidly crushed under the impact and friction of the supersonic gas flow, and cell-level honeysuckle powder is obtained;
[0076] Step S2, enzyme treatment: add deionized water with a pH of 6.5 to the honeysuckle powder and stir evenly, soak for 1 to 2 hours, and the amount of deionized water added is 10 to 15 times the weight of the honeysuckle powder; then add exoglucanase to the soaking liquid and transfer it to a closed container, and then stir evenly;
[0077] Step S3, ultrasonic treatment: the mixture after enzyme treatment is subjected to ultrasonic treatment at room temperature and at an ultrasonic frequency of 60 to 100 KHz for 25 to 30 minutes;
[0078] Step S4, filtering: filtering the mixture after ultrasonic treatment with a high-speed centrifuge or filtering out the filtrate with a plate and frame filter press;
[0079] Step S5, extraction: the filtered residue is forced to reflux with deionized water 6 to 12 times the weight of the residue for 1 to 1.5 hours, and the extraction is repeated 3 to 6 times to obtain an extract;
[0080] Step S6, concentration: combining the filtrate of step S4 and the extract of step S5 to obtain a chlorogenic acid initial solution, concentrating the chlorogenic acid initial solution under a vacuum condition of 0.05 MPa, adding chitosan 3% times the weight of the concentrated solution to the concentrated solution, stirring, and flocculating; then filtering, and decolorizing the filtrate with activated carbon to obtain a decolorized concentrated solution;
[0081] Step S7, purification: the concentrated solution after decolorization is saturatedly adsorbed by a strong polar macroporous macroporous resin column, eluted with ethanol water after adsorption, and the eluate is collected, concentrated, and dried to obtain a chlorogenic acid extract;
[0082] Step S8, chlorogenic acid extract liquid discharge: inject water into the bucket through the liquid inlet pipe 202, drive the juice in the filter barrel 901 to filter and flow, and the motor 7 drives the auger 602 to rotate in the pipeline 601 to stably transport the juice;
[0083] Step S9, cleaning: open the cover plate 201, open the barrel body 101, and move the filter barrel 901 to disassemble the filter barrel 901, so as to facilitate cleaning of the waste residue in the filter barrel 901.
Claims
1. A process and method for extracting chlorogenic acid from honeysuckle, characterized in that: The invention relates to an extraction device for extracting chlorogenic acid from honeysuckle, the extraction device also comprising a stirring barrel (1), a lid (2) being arranged above the stirring barrel (1), a stirrer (3) and a transmission device (4) being arranged above the lid (2), a crushing barrel (9) being arranged below the lid (2), and a discharge pipe (6) being arranged below the stirring barrel (1); The mixing barrel (1) comprises a barrel body (101), a limiting rod (102) and a groove one (103); the bottom of the barrel body (101) is arranged in an arc-shaped structure; the upper inner wall of the barrel body (101) is fixedly connected to the limiting rod (102); and the limiting rod (102) is provided with a groove one (103).
2. The extraction equipment for extracting chlorogenic acid from honeysuckle according to claim 1, characterized in that: The cover (2) comprises a cover plate (201), a liquid inlet pipe (202), a fixing clip (203) and a second groove (204); the cover plate (201) is engaged with the barrel mouth of the barrel body (101); the second groove (204) is provided on the bottom of the cover plate (201); the liquid inlet pipe (202) is fixedly connected to the top of the cover plate (201); and the liquid inlet pipe (202) is communicated with the inner cavity of the barrel body (101).
3. The extraction equipment for extracting chlorogenic acid from honeysuckle according to claim 2, characterized in that: A fixing card (203) is symmetrically fixedly connected to the cover plate (201), and a second gear (5) is rotatably connected in the middle of one side of the fixing card (203), and the second gear (5) passes through the cover plate (201).
4. The extraction equipment for extracting chlorogenic acid from honeysuckle according to claim 1, characterized in that: The transmission device (4) comprises a gear 1 (401), a rotating shaft (402) and a bevel gear 2 (403); the rotating shaft (402) is rotatably connected to the other side of the fixing card (203); one side of the rotating shaft (402) is fixedly connected to the gear 1 (401); the gear 1 (401) passes through the cover plate (201); and the other side of the rotating shaft (402) is fixedly connected to the bevel gear 2 (403).
5. The extraction equipment for extracting chlorogenic acid from honeysuckle according to claim 1, characterized in that: The stirrer (3) comprises a rotating rod (301), a stirring blade (302) and a bevel gear 1 (303); the upper side of the rotating rod (301) is rotatably connected to the cover plate (201); the upper side of the rotating rod (301) is fixedly connected to the bevel gear 1 (303); and the bevel gear 1 (303) is meshedly connected to the bevel gear 2 (403).
6. The extraction equipment for extracting chlorogenic acid from honeysuckle according to claim 5, characterized in that: The upper end of the rotating rod (301) is fixedly connected to the output end of the second motor (8), the side of the second motor (8) is fixedly connected to the cover plate (201), and a plurality of groups of stirring blades (302) are fixedly connected at equal angles on the rotating rod (301).
7. The extraction equipment for extracting chlorogenic acid from honeysuckle according to claim 1, characterized in that: The crushing barrel (9) comprises a filter barrel (901), an annular rack (902), an annular rod (903), a groove three (904) and a rotating wheel (905); the filter barrel (901) is embedded in the inner cavity of the barrel body (101); the barrel mouth of the filter barrel (901) is fixedly connected with an annular rack (902); the annular rack (902) is meshed with a gear one (401) and a gear two (5).
8. The extraction equipment for extracting chlorogenic acid from honeysuckle according to claim 7, characterized in that: The outer wall of the filter barrel (901) is fixedly connected to a ring rod (903), and four grooves (904) are formed at equal angles on the ring rod (903). A rotating wheel (905) is movable in each of the grooves (904). Both sides of the rotating wheel (905) are rotatably connected to the ring rod (903), and the lower side of the rotating wheel (905) is slidably connected to the groove (103).
9. The extraction equipment for extracting chlorogenic acid from honeysuckle according to claim 8, characterized in that: The discharge pipe (6) comprises a pipe (601) and an auger (602); the upper end of the pipe (601) is fixedly connected to the bottom of the barrel (101); the inner cavity of the pipe (601) is in communication with the inner cavity of the barrel (101); An auger (602) is movable in the pipe (601), one end of the auger (602) is rotatably connected to the pipe wall of the pipe (601), the edge of the auger (602) is closely connected to the inner wall of the pipe (601), and one end of the auger (602) passes through the pipe (601) and is fixedly connected to the output end of the motor 1 (7).
10. The method for extracting chlorogenic acid from honeysuckle according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step S1, crushing: Step S11, mechanical crushing: Step S111, pour honeysuckle into the filter barrel (901), embed the filter barrel (901) into the barrel body (101), and drive the rotating wheel (905) on the annular rod (903) to move, so that the rotating wheel (905) is slidably connected with the groove three (904), and the filter barrel (901) is limited and fixed while the filter barrel (901) is assisted to rotate; Step S112, the motor 2 (8) drives the rotating rod (301) to rotate, the rotating rod (301) drives the stirring blade (302) to rotate, and the honeysuckle in the filter barrel (901) is stirred and crushed, the rotating rod (301) drives the bevel gear 1 (303) to rotate, the bevel gear 1 (303) drives the bevel gear 2 (403) to rotate, the bevel gear 2 (403) drives the rotating shaft (402) to rotate, the rotating shaft (402) drives the gear 1 (401) to rotate on the annular rack (902), the annular rack (902) is fixedly connected to the barrel mouth of the filter barrel (901), and the gear 1 (401) drives the filter barrel (901) to rotate in the opposite direction to the stirring blade (302) to crush the honeysuckle; Step S12, freezing and crushing: fresh or dried honeysuckle material is placed in a sealed container made of stainless steel, and liquid nitrogen is passed through the container to freeze the material, so that the temperature of the honeysuckle material drops to -196°C and becomes a glass brittle state; a supersonic nitrogen gas flow is then passed through the sealed container, so that the low-temperature honeysuckle material is rapidly crushed under the impact and friction of the supersonic gas flow, and cell-level honeysuckle powder is obtained; Step S2, enzyme treatment: add deionized water with a pH of 6.5 to the honeysuckle powder and stir evenly, soak for 1 to 2 hours, and the amount of deionized water added is 10 to 15 times the weight of the honeysuckle powder; then add exoglucanase to the soaking liquid and transfer it to a closed container, and then stir evenly; Step S3, ultrasonic treatment: the mixture after enzyme treatment is subjected to ultrasonic treatment at room temperature and at an ultrasonic frequency of 60 to 100 KHz for 25 to 30 minutes; Step S4, filtering: filtering the mixture after ultrasonic treatment with a high-speed centrifuge or filtering out the filtrate with a plate and frame filter press; Step S5, extraction: the filtered residue is forced to reflux with deionized water 6 to 12 times the weight of the residue for 1 to 1.5 hours, and the extraction is repeated 3 to 6 times to obtain an extract; Step S6, concentration: combining the filtrate of step S4 and the extract of step S5 to obtain a chlorogenic acid initial solution, concentrating the chlorogenic acid initial solution under a vacuum condition of 0.05 MPa, adding chitosan 3% times the weight of the concentrated solution to the concentrated solution, stirring, and flocculating; then filtering, and decolorizing the filtrate with activated carbon to obtain a decolorized concentrated solution; Step S7, purification: the concentrated solution after decolorization is saturatedly adsorbed by a strong polar macroporous macroporous resin column, eluted with ethanol water after adsorption, and the eluate is collected, concentrated, and dried to obtain a chlorogenic acid extract; Step S8, chlorogenic acid extract discharge: inject water into the bucket through the liquid inlet pipe (202), drive the juice in the filter barrel (901) to filter and flow, and the motor 1 (7) drives the auger (602) to rotate in the pipeline (601) to stably transport the juice; Step S9, cleaning: open the cover plate (201), open the barrel body (101), and move the filter barrel (901) to disassemble the filter barrel (901), so as to facilitate cleaning of the waste residue in the filter barrel (901).
Citation Information
Patent Citations
Efficient extraction equipment for chlorogenic acid in stems and leaves of honeysuckle
CN213698900U