Negative pressure ultrafiltration centrifuge tube device

Abstract

The invention relates to the technical field of biological filtration, and particularly discloses a negative pressure ultrafiltration centrifuge tube device which comprises a negative pressure base, a negative pressure cover and an ultrafiltration bracket which are sequentially stacked and connected from bottom to top, the ultrafiltration support is provided with a plurality of placing holes arranged in a matrix, the placing holes are provided with ultrafiltration centrifuge tubes, a bottom frame is arranged in the negative pressure base, the bottom frame is provided with a plurality of groups of elastic limiting structures corresponding to the placing holes, and the elastic limiting structures clamp collecting tubes. The ultrafiltration centrifugal tubes and the corresponding collecting tubes are coaxially arranged, and at least part of each ultrafiltration centrifugal tube can extend into the corresponding collecting tube in the axial direction of the ultrafiltration centrifugal tube. Experiments such as liquid filtration, concentration, cell separation and desalination can be achieved through negative pressure, the application range is wide, compared with the prior art, the method has the advantages of being short in time and high in efficiency, damage and leakage of an ultrafiltration membrane cannot be caused, and cells can be effectively prevented from being damaged.

Description

Technical Field

[0001] The invention relates to the technical field of biological filtration, in particular to a negative pressure ultrafiltration centrifuge tube device. Background Art

[0002] Ultrafiltration centrifuge tube is a disposable filtration device that combines centrifugal force and ultrafiltration technology. It provides reliable data for the concentration, desalting and buffer exchange of protein samples in biological experiments. Compared with chromatography, dialysis and other methods, ultrafiltration centrifuge tubes are more gentle in handling molecules, do not require organic extraction, will not cause protein denaturation, and are fast, simple and efficient to operate. While separating molecules, the concentration multiple has been greatly improved, and the concentration efficiency has been significantly improved. Ultrafiltration is mainly used for desalting, diafiltration or replacement of buffers; concentration and purification of biological samples, including antigens, antibodies, enzymes, nucleic acids (single or double strain DNA / RNA samples), microorganisms, eluates, etc.; purification of macromolecular components in tissue culture extracts or cell lysates; removal of primers, connections or molecular markers from reaction mixtures, and removal of proteins before HPLC.

[0003] The existing ultrafiltration centrifuge tubes need to use a rotary high-speed centrifuge for centrifugal filtration, which takes a long time, has a limited number of centrifuges each time, and has low efficiency. In addition, high-speed centrifugal filtration can easily lead to damage and leakage of the ultrafiltration membrane, and even cause abnormal phenomena such as cell injury and damage. Summary of the invention

[0004] The technical problem to be solved by the present invention is: how to solve the problems of low efficiency and easy damage and leakage of ultrafiltration membrane in the prior art.

[0005] In order to solve the above technical problems, the present invention provides a negative pressure ultrafiltration centrifuge tube device, comprising a negative pressure base, a negative pressure cover and an ultrafiltration support stacked and connected in sequence from bottom to top;

[0006] The ultrafiltration support is provided with a plurality of placement holes arranged in a matrix, and the placement holes are provided with ultrafiltration centrifuge tubes. A base frame is provided in the negative pressure base, and the base frame is provided with a plurality of groups of elastic limiting structures corresponding to the placement holes, and the elastic limiting structures clamp the collection tube, and the ultrafiltration centrifuge tube is coaxially arranged with the corresponding collection tube, and at least part of the ultrafiltration centrifuge tube can extend into the collection tube along its axial direction; a first chamber is provided on the side of the negative pressure base facing the ultrafiltration support, and at least one protrusion structure for supporting the base frame is provided in the first chamber, and the protrusion structure is configured to form a gap between the base frame and the negative pressure base; a sealing ring is embedded in the placement hole, and the ultrafiltration centrifuge tube can be connected to the sealing ring; a first step structure is provided on the side of the negative pressure cover close to the ultrafiltration support, and a second step structure is provided on the side of the ultrafiltration support close to the negative pressure cover, and the first step structure and the second step structure are coupled to each other.

[0007] Further preferably, the sealing ring comprises a first abutting surface and a second abutting surface, the first abutting surface and the second abutting surface respectively abut against the outer surface and the inner surface of the ultrafiltration support, and one end of the first abutting surface and the second abutting surface are connected to form an embedding groove.

[0008] Further preferably, the inner surface of the ultrafiltration support is further provided with at least one first skeleton and at least one second skeleton, and the first skeleton and the second skeleton are arranged to intersect.

[0009] Further preferably, at least two protrusions are provided on the edge of the negative pressure base close to the negative pressure cover, and at least one cylindrical hole and at least one elliptical hole are provided on the edge of the negative pressure cover close to the negative pressure base, and the at least two protrusions are respectively plugged into the cylindrical hole and the elliptical hole.

[0010] Further preferably, the inner corners of the negative pressure cover are provided with hollowed-out rounded corners.

[0011] Further preferably, a sealing sheet is provided between the first step structure and the second step structure.

[0012] Further preferably, the sealing sheet has a thickness of 1-2 mm.

[0013] Further preferably, a second chamber is provided on the side of the base frame facing the ultrafiltration support, and a plurality of groups of the elastic limiting structures are arranged in a matrix in the second chamber, each group of the elastic limiting structures includes at least two arc columns, and the at least two arc columns are placed on the same circumference so as to be able to limit and clamp the collection tube.

[0014] Further preferably, the base frame is further provided with reinforcing ribs, and the reinforcing ribs are connected to the convex surface of the arc column.

[0015] Further preferably, the base frame is provided with a through hole connecting the first chamber and the second chamber.

[0016] Compared with the prior art, the negative pressure ultrafiltration centrifuge tube device provided by the present invention has the following beneficial effects:

[0017] The present invention provides a negative pressure base, a negative pressure cover and an ultrafiltration support which are stacked and connected in sequence from bottom to top. The placement holes arranged in a matrix can meet the filtering needs of multiple ultrafiltration centrifuge tubes at the same time, thereby improving the operating efficiency. After the ultrafiltration centrifuge tubes are placed in the placement holes accordingly, an independent and closed space is formed inside the negative pressure ultrafiltration centrifuge tube device. Under the action of negative pressure, the collection tube can independently collect the solution of each ultrafiltration centrifuge tube, thereby reducing the difference that may be caused by each tube and affecting the result, and facilitating independent observation and inspection. In addition, the elastic limiting structure provided on the bottom frame can fasten the collection tube to the bottom frame under negative pressure. At the same time, when the collection tube is inserted and pulled out, the elastic limiting structure will not break and will not cause the collection tube to be squeezed and broken. The convex structure can effectively The height of the base frame is raised to enhance the internal gas fluidity and improve the filtering effect; the sealing ring can fit with the ultrafiltration bracket to form a good sealing effect under negative pressure, and when the ultrafiltration centrifuge tube is pulled out, the sealing ring will not detach from the ultrafiltration bracket along with the ultrafiltration centrifuge tube; by setting the first step structure and the second step structure, the ultrafiltration bracket and the negative pressure cover are more convenient and flexible during installation, and can remain unchanged under negative pressure; the present invention can realize liquid filtration, concentration, cell separation, desalination and other experiments through negative pressure, and has a wide range of applications. Compared with the existing method of centrifugal filtration through a rotary high-speed centrifuge, the present invention has the advantages of short time and high efficiency, and will not cause damage or leakage of the ultrafiltration membrane, and can effectively prevent cells from being injured or damaged. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a stereoscopic diagram of the negative pressure ultrafiltration centrifuge tube device of the present invention.

[0019] Figure 2 It is a schematic diagram of an explosion of the negative pressure ultrafiltration centrifuge tube device of the present invention.

[0020] Figure 3 It is a structural schematic diagram of the negative pressure base of the present invention.

[0021] Figure 4 It is a structural schematic diagram of the negative pressure hood of the present invention.

[0022] Figure 5 It is a schematic diagram of the assembly of the negative pressure base and the negative pressure cover of the present invention.

[0023] Figure 6 It is a schematic structural diagram of the ultrafiltration support of the present invention.

[0024] Figure 7 It is a schematic structural diagram of the ultrafiltration support of the present invention from another perspective.

[0025] Figure 8 It is a cross-sectional view of the ultrafiltration support of the present invention.

[0026] Fig. 9The present invention Figure 8 A magnified schematic diagram of center A.

[0027] Fig.10 It is an exploded schematic diagram of the negative pressure cover and the ultrafiltration support of the present invention.

[0028] Fig.11 It is a schematic diagram of the assembly of the base frame and the collecting pipe of the present invention.

[0029] Fig.12 It is an exploded schematic diagram of the base frame and the collecting pipe of the present invention.

[0030] Fig.13 It is a top view of the base frame of the present invention.

[0031] Fig.14 It is a top view of the negative pressure ultrafiltration centrifuge tube device of the present invention.

[0032] Fig.15 The present invention Fig.14 Cross-sectional view of section BB.

[0033] Fig.16 The present invention Fig.14 Cross-sectional view of section CC.

[0034] Reference numerals:

[0035] 10. Negative pressure base; 101. First chamber; 102. Connecting hole; 103. Boss; 104. Protruding structure;

[0036] 20. Negative pressure cover; 201. Cylindrical hole; 202. Elliptical hole; 203. Hollowed-out fillet; 204. First step structure;

[0037] 30, ultrafiltration support; 301, placement hole; 302, sealing ring; 302a, first abutting surface; 302b, second abutting surface; 302c, embedded groove; 303, first frame; 304, second frame; 305, second step structure;

[0038] 40. Ultrafiltration centrifuge tube;

[0039] 50, base frame; 501, second chamber; 502, arc column; 503, through hole; 504, reinforcing rib;

[0040] 60. Collection tube;

[0041] 70. Sealing piece. DETAILED DESCRIPTION

[0042] The specific implementation of the present invention is further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0043] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like used in the present invention to indicate the orientation or position relationship is based on the orientation or position relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention.

[0044] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0045] In addition, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0046] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0047] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation method.

[0048] like Figure 1-Figure 16 As shown, this embodiment provides a negative pressure ultrafiltration centrifuge tube device, including a negative pressure base 10, a negative pressure cover 20, an ultrafiltration bracket 30, an ultrafiltration centrifuge tube 40, a base frame 50 and a collection tube 60, wherein the negative pressure base 10 is connected to the negative pressure system. This embodiment can realize liquid filtration, concentration, cell separation, desalination and other experiments through negative pressure.

[0049] In some embodiments, the negative pressure base 10, the negative pressure cover 20 and the ultrafiltration support 30 are stacked and connected in sequence from bottom to top, the base frame 50 is arranged in the negative pressure base 10, the ultrafiltration support 30 is used to fix the ultrafiltration centrifuge tube 40, and the base frame 50 is used to fix the collection tube 60.

[0050] In some embodiments, a first chamber 101 is provided on the side of the negative pressure base 10 facing the ultrafiltration support 30, and a connecting hole 102 connected to the first chamber 101 is provided on the side wall of the negative pressure base 10. The connecting hole 102 is connected to the negative pressure system. When the negative pressure system is configured to be started, a negative pressure state can be formed in the first chamber 101, thereby enabling experiments such as liquid filtration, concentration, cell separation, and desalination in the ultrafiltration centrifuge tube 40.

[0051] In some embodiments, the ultrafiltration support 30 is provided with a plurality of placement holes 301 arranged in a matrix, and the ultrafiltration centrifuge tube 40 can be fixed in the placement hole 301 along the axial direction, thereby satisfying the filtration use of a plurality of ultrafiltration centrifuge tubes 40 at the same time and improving the operation efficiency.

[0052] In some embodiments, the base frame 50 is provided with a plurality of groups of elastic limiting structures corresponding to the placement holes 301, and the elastic limiting structures clamp the collection tube 60. The elastic limiting structures can fasten the collection tube 60 to the base frame 50 under a negative pressure state. At the same time, when the collection tube 60 is inserted and pulled out, the elastic limiting structure will not break, nor will it cause the collection tube 60 to be squeezed; the ultrafiltration centrifuge tube 40 is coaxially arranged with the corresponding collection tube 60, and at least a portion of the ultrafiltration centrifuge tube 40 can extend into the collection tube 60 along its axial direction, so that the collection tube 60 can effectively collect the substances filtered out by the ultrafiltration centrifuge tube 40.

[0053] Compared with centrifugal filtration using a rotary high-speed centrifuge, this embodiment has the advantages of short time and high efficiency, and will not cause damage or leakage of the ultrafiltration membrane, thus effectively preventing cells from being injured or damaged.

[0054] In some embodiments, in order to enable the negative pressure ultrafiltration centrifuge tube device to form an independent and closed space inside when it is working, for this purpose, Figure 6-Figure 9 As shown, a sealing ring 302 is embedded in the placement hole 301, and the ultrafiltration centrifuge tube 40 can be connected to the sealing ring 302, and the ultrafiltration centrifuge tube 40 and the sealing ring 302 are interference connected. Therefore, when the ultrafiltration centrifuge tube 40 is connected to the sealing ring 302, the placement hole 301 can be completely sealed to prevent external gas from entering the interior and affecting the experimental results.

[0055] In other embodiments, the sealing ring 302 is preferably a medical-grade silicone ring to avoid contamination of the specimen.

[0056] In addition, during the insertion and removal process, the sealing ring 302 can produce elastic deformation without damaging the ultrafiltration centrifuge tube 40, thereby avoiding damage caused by friction between the ultrafiltration centrifuge tube 40 and the ultrafiltration bracket 30 during the insertion and removal process, and the sealing ring 302 can be interference connected with the ultrafiltration centrifuge tube 40, and the sealing ring 302 holds the ultrafiltration centrifuge tube 40 tightly, further improving the reliability of the experimental results.

[0057] In some embodiments, the sealing ring 302 includes a first abutting surface 302a and a second abutting surface 302b, and the first abutting surface 302a and the second abutting surface 302b are respectively abutted against the outer surface and the inner surface of the ultrafiltration support 30, and one end of the first abutting surface 302a and the second abutting surface 302b are connected to form an embedding groove 302c; in this way, the sealing ring 302 adopts an embedded structure to be more firm and durable. When the interior of the negative pressure ultrafiltration centrifuge tube device is in a negative pressure state, the outer pressure of the sealing ring 302 is greater than the inner pressure. At this time, the first abutting surface 302a fits with the outer surface of the ultrafiltration support 30 to form a good sealing effect. When the ultrafiltration centrifuge tube 40 is pulled out, the second abutting surface 302b fits with the inner surface of the ultrafiltration support 30, which plays a role in limiting the sealing ring 302. The sealing ring 302 will not detach from the ultrafiltration support 30 along with the ultrafiltration centrifuge tube 40. Replacing a new ultrafiltration centrifuge tube 40 can perform negative pressure filtration again without replacing the sealing ring 302, which is convenient and quick.

[0058] In some embodiments, the inner surface of the ultrafiltration support 30 is also provided with at least one first skeleton 303 and at least one second skeleton 304, and the first skeleton 303 and the second skeleton 304 are arranged to intersect each other; by setting the first skeleton 303 and the second skeleton 304, the structural strength of the ultrafiltration support 30 can be effectively enhanced. On the one hand, the ultrafiltration support 30 will not be deformed under the action of negative pressure. On the other hand, the ultrafiltration support 30 will not be damaged when the ultrafiltration centrifuge tube 40 is inserted and removed.

[0059] In other embodiments, the first skeleton 303 and the second skeleton 304 intersect perpendicularly.

[0060] In other embodiments, the ultrafiltration support 30 is an aluminum alloy support, which can improve the structural strength of the ultrafiltration support 30 when combined with the skeleton.

[0061] In some embodiments, Figure 3 As shown, at least one protruding structure 104 for supporting the base frame 50 is provided in the first chamber 101 of the negative pressure base 10. The protruding structure 104 is configured to form a gap between the base frame 50 and the negative pressure base 10, which can effectively raise the height of the base frame 50, enhance the internal gas flowability and improve the filtering effect.

[0062] In some embodiments, a second chamber 501 is provided on the side of the base frame 50 facing the ultrafiltration support 30, and the base frame 50 is provided with a through hole 503 connecting the first chamber 101 and the second chamber 501, so as to more effectively form air pressure circulation and further enhance the internal gas fluidity.

[0063] In some embodiments, in order to further improve the effect of smooth air circulation, a hollowed-out rounded corner 203 is provided at the inner corner of the negative pressure cover 20 .

[0064] In some embodiments, Figure 3-Figure 5 As shown, at least two bosses 103 are provided on the edge of the negative pressure base 10 close to the negative pressure cover 20, and at least one cylindrical hole 201 and at least one elliptical hole 202 are provided on the edge of the negative pressure cover 20 close to the negative pressure base 10. The at least two bosses 103 are respectively plugged into the cylindrical hole 201 and the elliptical hole 202, so that the negative pressure base 10 and the negative pressure cover 20 can be accurately plugged into each other, and the rotation pair between the negative pressure base 10 and the negative pressure cover 20 can be limited.

[0065] Specifically, the present embodiment preferably has two bosses 103, and the two bosses 103 are respectively arranged on opposite sides of the negative pressure base 10, and the cylindrical hole 201 and the elliptical hole 202 are also respectively arranged on opposite sides of the negative pressure cover 20. When the negative pressure base 10 and the negative pressure cover 20 are connected, one of the bosses 103 is plugged into the cylindrical hole 201, and the other boss 103 is plugged into the elliptical hole 202. The setting of the elliptical hole 202 enables the negative pressure cover 20 to adapt to the negative pressure base 10 more flexibly, thereby improving assembly efficiency.

[0066] In some embodiments, Fig.10As shown, a first step structure 204 is provided on the side of the negative pressure cover 20 close to the ultrafiltration support 30, and a second step structure 305 is provided on the side of the ultrafiltration support 30 close to the negative pressure cover 20. The first step structure 204 and the second step structure 305 are coupled to each other. By setting the first step structure 204 and the second step structure 305, the ultrafiltration support 30 and the negative pressure cover 20 are more convenient and flexible to install, and can remain unchanged under negative pressure.

[0067] In some embodiments, in order to further improve the airtightness of the connection between the negative pressure cover 20 and the ultrafiltration support 30, a sealing sheet 70 is provided between the first step structure 204 and the second step structure 305, so that under the negative pressure state, a downward force is applied to the ultrafiltration support 30, and the first step structure 204 and the second step structure 305 are tightly connected under the action of the sealing sheet 70 to form a sealed state.

[0068] In other embodiments, the sealing sheet 70 is preferably a medical silicone sheet to avoid contamination of the specimen.

[0069] In some embodiments, the thickness of the sealing sheet 70 is 1-2 mm, preferably 1.5 mm.

[0070] In some embodiments, the negative pressure cover 20 is a plexiglass negative pressure cover, which has the advantages of transparency and toughness, which is convenient for observation and can improve the coordination effect between the negative pressure cover 20 and the ultrafiltration support 30.

[0071] In some embodiments, Figure 11-13 As shown, a second chamber 501 is provided on the side of the base frame 50 facing the ultrafiltration support 30, and a plurality of groups of elastic limiting structures are arranged in a matrix in the second chamber 501, wherein each group of elastic limiting structures can independently fix a collecting tube 60 to avoid displacement of the collecting tube 60, and at the same time can reduce the difference that may be caused by each collecting tube 60 and affect the results, thereby facilitating independent observation and inspection.

[0072] In some embodiments, each group of elastic limiting structures includes at least two arc columns 502, and the present embodiment preferably includes three arc columns 502. The three arc columns 502 are placed on the same circumference, and the three arc columns 502 are surrounded to be able to limit and clamp the collection tube 60, so that the collection tube 60 can be fastened to the base frame 50 under negative pressure.

[0073] In some embodiments, when inserting and removing the collecting tube 60, in order to effectively prevent the arc column 502 from breaking or the collecting tube 60 from being squeezed, the base frame 50 is also provided with a reinforcing rib 504, which is connected to the convex surface of the arc column 502, thereby enhancing the structural strength of the arc column 502.

[0074] The working process of the present invention is: please combine Figure 1-Figure 16When in use, first fix the collection tube 60 on the elastic limiting structure of the base frame 50, then place the base frame 50 on the negative pressure base 10, plug the cylindrical hole 201 and the elliptical hole 202 of the negative pressure cover 20 into the convex columns 103 at both ends of the negative pressure base 10 respectively, connect the ultrafiltration centrifuge tube 40 to the sealing ring 302, connect the second step structure 305 of the ultrafiltration support 30 to the first step structure 204 of the negative pressure cover 20, adjust the position of the ultrafiltration centrifuge tube 40 so that at least part of it can extend axially into the collection tube 60, connect the external negative pressure system to the connecting hole 102, and after starting the negative pressure system, the substance in the ultrafiltration centrifuge tube 40 can realize liquid filtration, concentration, cell separation, desalination and other experiments under negative pressure.

[0075] In summary, the negative pressure ultrafiltration centrifuge tube device provided by the present invention can realize experiments such as liquid filtration, concentration, cell separation, and desalination through negative pressure, and has a wide range of applications. Compared with the existing method of centrifugal filtration through a rotary high-speed centrifuge, the present invention has the advantages of short time and high efficiency, and will not cause damage or leakage of the ultrafiltration membrane, and can effectively prevent cell damage.

[0076] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and substitutions can be made without departing from the technical principles of the present invention, and these improvements and substitutions should also be regarded as the protection scope of the present invention. The basic principles, main features and advantages of the present invention are shown and described above. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above preferred embodiments. The embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, so it is intended to include all changes within the meaning and scope of the equivalent elements of the claims in the present invention.

[0077] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in the embodiments may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A negative pressure ultrafiltration centrifuge tube device, characterized in that: It includes a negative pressure base, a negative pressure cover and an ultrafiltration support which are stacked and connected in sequence from bottom to top; The ultrafiltration support is provided with a plurality of placement holes arranged in a matrix, and the placement holes are provided with ultrafiltration centrifuge tubes. A base frame is provided in the negative pressure base, and the base frame is provided with a plurality of groups of elastic limiting structures corresponding to the placement holes, and the elastic limiting structures clamp the collection tube, and the ultrafiltration centrifuge tube is coaxially arranged with the corresponding collection tube, and at least part of the ultrafiltration centrifuge tube can extend into the collection tube along its axial direction; a first chamber is provided on the side of the negative pressure base facing the ultrafiltration support, and at least one protrusion structure for supporting the base frame is provided in the first chamber, and the protrusion structure is configured to form a gap between the base frame and the negative pressure base; a sealing ring is embedded in the placement hole, and the ultrafiltration centrifuge tube can be connected to the sealing ring; a first step structure is provided on the side of the negative pressure cover close to the ultrafiltration support, and a second step structure is provided on the side of the ultrafiltration support close to the negative pressure cover, and the first step structure and the second step structure are coupled to each other.

2. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: The sealing ring comprises a first abutting surface and a second abutting surface, the first abutting surface and the second abutting surface respectively abut against the outer surface and the inner surface of the ultrafiltration support, and one end of the first abutting surface and the second abutting surface are connected to form an embedding groove.

3. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: The inner surface of the ultrafiltration support is also provided with at least one first frame and at least one second frame, and the first frame and the second frame are arranged to intersect.

4. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: The edge of the negative pressure base close to the negative pressure cover is provided with at least two protrusions, and the edge of the negative pressure cover close to the negative pressure base is provided with at least one cylindrical hole and at least one elliptical hole, and the at least two protrusions are respectively plugged into the cylindrical hole and the elliptical hole.

5. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: The inner corners of the negative pressure cover are provided with hollowed-out rounded corners.

6. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: A sealing sheet is provided between the first step structure and the second step structure.

7. A negative pressure ultrafiltration centrifuge tube device according to claim 6, characterized in that: The thickness of the sealing sheet is 1-2 mm.

8. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: A second chamber is provided on the side of the base frame facing the ultrafiltration support, and multiple groups of elastic limiting structures are arranged in a matrix in the second chamber, each group of the elastic limiting structures includes at least two arc columns, and the at least two arc columns are placed on the same circumference to be able to limit and clamp the collection tube.

9. A negative pressure ultrafiltration centrifuge tube device according to claim 8, characterized in that: The base frame is also provided with reinforcing ribs, and the reinforcing ribs are connected to the convex surface of the arc column.

10. The negative pressure ultrafiltration centrifuge tube device according to claim 8, characterized in that: The base frame is provided with a through hole communicating with the first cavity and the second cavity.

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