Soil sample preservation method and device

Abstract

The invention belongs to the technical field of soil sample preservation, relates to a soil sample preservation method and device, and aims to provide an efficient, reliable and environment-friendly soil sample long-term preservation solution. The method comprises the following steps: preheating a sample container, adding a soil sample, quickly sealing, cooling and storing, keeping out of light in the whole process, and forming 0.7-0.9 atm negative pressure; the device comprises an outer bottle, an inner bottle, a bottle cap, a high-temperature box and a low-temperature box. By accurately controlling the temperature and the negative pressure, the sample storage efficiency and reliability are improved, meanwhile, the environmental pollution and the operation risk are reduced, and the stability and the analysis accuracy of the soil sample are ensured. The method and the device are easy to operate, dependence on professional skills is reduced, material waste and equipment maintenance cost are reduced, and good economical efficiency and practicability are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of soil sample preservation, and relates to a soil sample preservation method and a soil sample preservation device suitable for the method. Background Art

[0002] The long-term preservation of soil samples plays a vital role in soil research, environmental monitoring and agricultural development. The preservation quality of these samples directly affects the accuracy and reliability of subsequent analysis and research. In the third national soil survey and agricultural soil research, a large number of soil specimens need to be collected and preserved for a long time, with a preservation time of no less than 2 years, to facilitate future analysis and research. The traditional preservation method mainly relies on manual wax sealing operations, which includes steps such as melting wax, applying wax seals, cooling and solidifying, checking seals, and marking and recording.

[0003] Although the wax sealing method can achieve the sealed preservation of soil samples to a certain extent, it has many shortcomings. First, the wax sealing process requires manual operation, which is inefficient and time-consuming for large-scale soil sample processing. Secondly, the manual application of wax seals can easily lead to uneven thickness, affecting the consistency of the sealing effect. In addition, wax sealing requires heating to melt the wax material, which poses a risk of burns and fire. At the same time, smoke and volatile substances may be generated during the heating process, which will affect the environment and the health of operators.

[0004] Traditional wax sealing methods also involve the risk of sample contamination. Manual operation may also cause the mouth of the sample bottle to come into contact with contaminants, increasing the possibility of sample contamination. In addition, the sealing effect of the wax seal is affected by many factors, such as the quality of the wax and the application technique, which may lead to a loose seal and affect the long-term preservation of the sample. The process of melting the sealing wax is complicated and requires additional heating equipment and time, which increases the difficulty of sampling. In the process of applying and removing the wax seal, there may be a waste of wax material, and the heating equipment and tools used need to be cleaned and maintained regularly, increasing the workload.

[0005] During manual operation, it is difficult to record and trace the wax seal status of each sample, which is not conducive to sample management. In addition, operators need to be specially trained to ensure that they can perform wax sealing operations correctly. These training and skill requirements increase the overall operation cost and complexity. Summary of the invention

[0006] In view of this, the purpose of the present invention is to provide a new method for long-term preservation of soil samples and a device suitable for the method, so as to solve the above-mentioned problems, improve the sealing efficiency, improve the uniformity of the sealing effect, and make sampling more convenient and quick, while reducing environmental pollution and operational risks, and improving the safety and reliability of sample preservation.

[0007] The inventor has continuously innovated and reformed through long-term exploration and attempts, as well as multiple experiments and efforts. To solve the above technical problems, the present invention provides a method for preserving soil samples, comprising the following steps: S1. Preheat the sample container. The air temperature in the sample container is 50℃~100℃; S2. Take the preheated sample container, add the soil sample, and quickly cover the container to seal the sample container; S3. Cool the sample container containing soil to 0℃~25℃, create negative pressure inside the sample container, and store it at 0℃~25℃ away from light.

[0008] Preferably, in step S1, the air temperature in the sample container is 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C, 90°C, 95°C, two endpoint values ​​in the range of 50°C to 100°C and any temperature value between the two endpoint values.

[0009] Preferably, in step S3, the sample container is cooled to 0°C, 4°C, 5°C, 10°C, 15°C, 20°C, 25°C, and any temperature value between the two endpoints of 0°C-25°C.

[0010] Compared with the prior art, the present invention has the following beneficial effects: Compared with the prior art, the beneficial effect of the present invention is that it provides an efficient, reliable and environmentally friendly soil sample preservation method. Utilizing the ideal gas law PV=nRT, by controlling the temperature change in the sample container to form a negative pressure environment, this method not only improves the sealing efficiency and effect, but also ensures the uniformity of the sealing effect. In addition, this method avoids the problems of low efficiency, inconsistent sealing effect, potential safety hazards, environmental pollution, and sample contamination in traditional wax sealing operations. By storing in the dark within a temperature range of 0°C to 25°C, the present invention further ensures the stability and integrity of the soil sample, reduces sample degradation and changes, and thus provides more accurate and reliable data for subsequent analysis and research. In addition, this method simplifies the sampling process, reduces the difficulty of operation, reduces dependence on professional skills, and also reduces material waste and equipment maintenance costs, and has good economy and practicality.

[0011] On the basis of the above technical solution, the present invention can also be improved as follows: Further: It also includes the sampling steps: S4. Take the sample container stored under negative pressure, reheat it, eliminate the negative pressure inside the sample container, open the lid, and take a sample for sample testing.

[0012] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: The present invention further realizes rapid and convenient sampling of soil samples, greatly improving the working efficiency of sample detection. The negative pressure inside the sample container is eliminated through the reheating step, making the bottle cap easy to open, thereby avoiding the complicated sampling pre-treatment process in the traditional method, such as melting the sealing wax, etc., reducing the sampling time and labor intensity. In addition, this improved method reduces the risk of contamination to the sample due to the complicated sampling process, ensuring the purity of the sample and the accuracy of the analysis results.

[0013] On the basis of the above technical solution, the present invention can also be improved as follows: Further: The sample container comprises an outer bottle, an inner bottle and a bottle cap; an outer cap matched with the outer bottle and an inner cap matched with the inner bottle are arranged on the same surface of the bottle cap.

[0014] Preferably, the soil sample is first placed in an inner bottle, which is sealed with a lid and stored at room temperature or at a low temperature of 0°C to 25°C.

[0015] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: The present invention further significantly improves the safety and sealing of soil sample storage. By designing a sample container including an outer bottle, an inner bottle, and an outer cover and an inner cover respectively matched therewith, the present invention realizes a double-layer sealing structure, thereby enhancing the sealing effect of the sample container and effectively preventing the sample from being disturbed and contaminated by the external environment. The inner bottle design significantly improves the efficiency and safety of soil sample storage. This design allows the operator to pre-load the soil sample into the inner bottle and seal it at room temperature or low temperature, avoiding direct processing of the sample at high temperature. At the same time, the use of the inner bottle speeds up the speed of placing the sample into the outer bottle. The use of the inner bottle effectively protects the soil sample from the direct influence of the high temperature of the outer bottle, reduces the possible influence of temperature changes on the sample, and maintains the stability of the sample. At the same time, this design reduces the risk of contamination of the sample during the transfer process, because the bottle cap of the inner bottle plays a protective role before being loaded into the outer bottle.

[0016] On the basis of the above technical solution, the present invention can also be improved as follows: Further: The outer bottle is a transparent glass container or a metal container, and the inner bottle is an opaque or translucent glass container; the volume of the outer bottle is 10% to 40% greater than the volume of the inner bottle.

[0017] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: By designing the outer bottle as a transparent glass container, the operator can intuitively observe the status of the inner bottle and soil samples, which is convenient for monitoring the preservation of the samples and performing necessary operations. At the same time, the inner bottle uses an opaque or translucent glass container, which helps protect the soil samples from light, especially for light-sensitive samples, this design can maintain their stability and integrity. In addition, the volume of the outer bottle is 10% to 40% larger than that of the inner bottle. This design provides sufficient space for thermal expansion and contraction. At the same time, this volume difference also helps to form and maintain a negative pressure environment, making the sample container more tightly sealed and more effectively protecting the soil samples from the external environment.

[0018] On the basis of the above technical solution, the present invention can also be improved as follows: Further: A soil sample information label is arranged on the outer wall of the inner bottle.

[0019] Preferably, the soil sample information identifier includes but is not limited to key information such as sample name, collection location, collection date, and sample number.

[0020] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: By adopting this further improved technical solution, the soil sample information label is directly placed on the outer wall of the inner bottle, that is, inside the outer bottle. This design has significant benefits compared to the prior art. First, placing the information label inside the outer bottle avoids the risk of information loss or damage due to external environmental factors (such as weather, physical damage, chemical oxidation, etc.), ensuring the long-term preservation and traceability of sample information. Secondly, this built-in information identification method reduces the reliance on external labels or records, reduces confusion caused by label shedding or recording errors, and improves the accuracy and efficiency of sample management. In addition, the design of the built-in information label also makes the appearance of the sample container neater, which is convenient for unified management and identification. This improved technical solution not only enhances the stability and reliability of sample information by embedding the soil sample information label inside the outer bottle, but also improves the convenience and safety of overall sample storage and management.

[0021] On the basis of the above technical solution, the present invention can also be improved as follows: Further: A sealing ring is arranged inside the outer cover.

[0022] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: Providing a sealing ring inside the outer cover can effectively improve the sealing performance of the sample container and reduce the intrusion of external gases and pollutants, thereby ensuring the long-term stability and analysis accuracy of the soil sample.

[0023] On the basis of the above technical solution, the present invention can also be improved as follows: Further: The outer wall of the inner bottle is also provided with a heat insulation layer.

[0024] Preferably, the heat insulating layer includes but is not limited to one of a foam material layer, a microporous polymer material layer, a glass fiber layer, and a natural fiber layer.

[0025] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: An insulating layer is arranged on the outer wall of the inner bottle, which can effectively isolate the hot air in the outer bottle from directly affecting the soil sample when the inner bottle is placed in the outer bottle, thereby reducing the interference of temperature fluctuations on the stability of the sample.

[0026] On the basis of the above technical solution, the present invention can also be improved as follows: Further: The negative pressure is 0.7~0.9atm.

[0027] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: The negative pressure range is set to 0.7~0.9atm, which significantly increases the force required to open the sample container, thereby enhancing the safety of the sample container. Since a larger force is required to break the negative pressure and open the container, this effectively prevents unauthorized access or accidental opening, ensuring the integrity and safety of the soil sample.

[0028] The present invention also provides a device for the soil sample preservation method, comprising: The sample container comprises an outer bottle, an inner bottle and a bottle cap, wherein an outer cap matching with the outer bottle and an inner cap matching with the inner bottle are arranged on the same surface of the bottle cap; a sealing ring is arranged inside the outer cap; the inner bottle is used to hold the soil sample, and the outer bottle and the outer cap constitute a negative pressure storage assembly; A high temperature box, having a first inner cavity for accommodating at least one outer bottle, for preheating or reheating the outer bottle of the sample container, so that the air temperature in the bottle is heated to 50°C to 100°C; The low temperature box has a second inner cavity for accommodating at least one inner bottle, and is used to cool the sample container containing the soil sample to 0°C~25°C, so that a negative pressure of 0.7~0.9atm is formed in the outer bottle.

[0029] Compared with the prior art, the present invention has the following beneficial effects: Compared with the prior art, the device provided by the present invention realizes precise temperature control during the preservation of soil samples through a high temperature box and a low temperature box, thereby significantly improving the efficiency and reliability of sample preservation. The high temperature box is used to preheat or reheat the outer bottle of the sample container to ensure that the air temperature in the bottle reaches 50°C~100°C, so that the air expands; while the low temperature box is used to quickly cool the sample container containing the soil sample to 0°C~25°C, so that the air shrinks and forms the required 0.7~0.9atm negative pressure environment. This temperature control method not only simplifies the operation process and reduces human errors, but also improves the consistency and repeatability of sample preservation. In addition, by precisely controlling the negative pressure, the present invention enhances the sealing of the sample container, effectively prevents the sample from being damp, oxidized or contaminated by microorganisms, and ensures the long-term stability of the soil sample and the accuracy of the analysis. At the same time, the design of this device also improves the safety of the sample container, because a large force is required to break the negative pressure and open the container, thereby preventing unauthorized access or accidental opening, and protecting the safety of the sample and the operator. In summary, the device of the present invention provides an efficient, safe and reliable solution for the long-term preservation of soil samples through its unique design and function. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0031] Figure 1 It is a structural schematic diagram of a preferred embodiment of the soil sample storage device of the present invention.

[0032] Figure 2 It is a schematic diagram of the front view structure of the sample container in a preferred embodiment of the soil sample storage device of the present invention.

[0033] Figure 3 yes Figure 2 Schematic diagram of the top view structure.

[0034] Figure 4 yes Figure 2 Schematic diagram of the three-dimensional structure.

[0035] Figure 5 yes Figure 2 Schematic diagram of the AA cross-section in FIG.

[0036] The marks in the figure are: 100 sample containers, 110 inner bottles, 111 insulation layer, 112 Information identification, 120 outer bottles, 130 bottle caps, 131 inner cover, 132 outer cover, 133 sealing ring, 200 high temperature box, 300 cryogenic chamber, 400 soil samples. DETAILED DESCRIPTION

[0037] The following is a description with reference to the accompanying drawings and a specific embodiment.

[0038] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention.

[0039] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and thus, once an item is defined in one drawing, it may not be further defined or explained in the subsequent drawings.

[0040] Example 1 This embodiment describes a soil sample preservation method using a conventional soil sample container, which is simple, practical, and suitable for most laboratory environments. Conventional sample containers are such as brown glass containers.

[0041] Step S1: Preheating the sample container In this embodiment, the conventional sample container needs to be preheated first. Use a common drying oven or constant temperature oven in the laboratory to preheat the sample container to a temperature range of 50°C to 100°C. The purpose of this step is to heat and expand the air in the sample container to prepare for the formation of negative pressure in the subsequent steps.

[0042] Step S2: Add soil sample and seal the sample container After preheating is completed, take out the preheated sample container from the high temperature box and quickly add the soil sample into the inner bottle. To ensure the purity of the sample and avoid contamination, the operation should be carried out under sterile conditions. After adding the soil sample, immediately tighten the bottle cap to close the sample container.

[0043] Step S3: Cool down to form negative pressure and save After sealing the sample container, place it in a cryogenic box for cooling. The temperature of the cryogenic box is set between 0℃ and 25℃ to ensure that the sample container cools down quickly. The faster the cooling rate, the less thermal impact on the soil sample. As the outer bottle cools, the air inside the bottle contracts, forming a negative pressure environment of 0.7~0.9atm. During this process, the sample container should be kept away from light to protect the soil sample from light. The formation of negative pressure helps to enhance the sealing of the sample container and reduce the impact of the external environment on the soil sample.

[0044] Through the above steps, this embodiment provides a soil sample preservation method using a conventional sample container. The method is easy to operate, can effectively maintain the stability and integrity of the soil sample, and is suitable for occasions where soil samples need to be preserved for a long time.

[0045] Example 2 Based on Example 1, this example adds a step of reheating the sample container.

[0046] When sampling is required, the sample container stored under negative pressure is taken out from the low temperature box and placed in the high temperature box for reheating. The purpose of reheating is to eliminate the negative pressure inside the sample container and restore the air pressure in the outer bottle to normal atmospheric pressure. This step is usually carried out at a temperature range of 50℃~100℃ until the pressure inside the container returns to 1atm. After reheating, the outer bottle cap can be easily opened to perform soil sample sampling.

[0047] Example 3 This embodiment will use the Figures 1 to 5 The sample container 100 shown is used to perform the preservation method of the soil sample 400. These sample containers 100 are designed to optimize the preservation conditions of the soil sample 400, and include an outer bottle 120, an inner bottle 110 and a bottle cap 130 with special functions.

[0048] Step 1: Preheat the outer bottle to 120 Using the high temperature box 200 provided by the present invention, the outer bottle 120 of the sample container 100 is placed in the high temperature box 200 for preheating. The outer bottle 120 is heated to 50°C to 100°C in the high temperature box 200, so that the air in the bottle expands to prepare for the formation of negative pressure.

[0049] Step 2: Load soil sample 400 The soil sample 400 is placed in the inner bottle 110, which is sealed with the inner cap 131 and stored at room temperature or 0° C. to 25° C. The inner bottle 110 is designed as an opaque or translucent glass container, preferably a brown glass bottle, and the outer wall is provided with an insulation layer 111 and a soil sample 400 information label 112 for easy recording and tracing.

[0050] Step 3: Add soil sample 400 and seal sample container 100 First, take out the inner bottle 110. After the outer bottle 120 is preheated, take out the outer bottle 120 from the high temperature box 200, and immediately put the inner bottle 110 containing the soil sample 400 into the outer bottle 120, and tighten the outer cover 132. A sealing ring 133 is provided inside the outer cover 132 to ensure a good sealing effect.

[0051] Step 4: Cool down to create negative pressure and save After the sample container 100 is sealed, it is placed in a low temperature box 300 for cooling. The temperature of the low temperature box 300 is set between 0°C and 25°C to quickly cool the sample container 100 and form a negative pressure environment of 0.7 to 0.9 atm. During this process, the sample container 100 should be kept away from light to protect the soil sample 400 from being affected by light.

[0052] In some embodiments, it also includes Step 5: Sampling When sampling is required, the sample container 100 stored under negative pressure is taken out from the low temperature box 300 and placed in the high temperature box 200 for reheating to eliminate the internal negative pressure. After reheating, the air pressure in the outer bottle 120 returns to normal atmospheric pressure, so that the cover of the outer bottle 120 can be easily opened to perform the sampling operation of the soil sample 400.

[0053] By using the sample container 100 provided by the present invention, this embodiment not only ensures the stability and integrity of the soil sample 400 during the storage process, but also improves the convenience and safety of operation. This improved method is suitable for soil samples 400 that need long-term storage and frequent sampling, and improves the efficiency and reliability of soil sample 400 processing.

[0054] Example 4 This embodiment describes in detail a soil sample 400 storage device, see Figure 1~Figure 5 , the device is designed to achieve long-term preservation of soil samples 400 while ensuring the stability and safety of the samples.

[0055] Sample container 100: It includes an outer bottle 120 and an inner bottle 110, and an integrated bottle cap 130 system.

[0056] The inner bottle 110 is designed as an opaque or translucent glass container for containing the soil sample 400. The outer wall is provided with an information label 112 of the soil sample 400, including the sample name, collection location, collection date, sample number, etc. The outer wall is also provided with a heat insulation layer 111 to reduce the impact of temperature changes on the soil sample 400.

[0057] The outer bottle 120 is a transparent glass container, which is used to cover the inner bottle 110 and form a negative pressure storage environment.

[0058] The bottle cap 130 system is provided with an outer cap 132 matched with the outer bottle 120 and an inner cap 131 matched with the inner bottle 110 on the same surface, and the outer cap 132 is coaxially arranged with the inner cap 131. A sealing ring 133 is arranged inside the outer cap 132 to ensure the sealing of the container.

[0059] High temperature box 200: The outer bottle 120 used to preheat or reheat the sample container 100 is heated to 50°C~100°C. The high temperature box 200 is designed with at least one inner cavity for accommodating the outer bottle 120 to ensure that the outer bottle 120 can be completely heated. The box body is usually made of heat-resistant material to ensure safety and durability at high temperatures. As a preferred embodiment, the high temperature box 200 is also provided with a temperature control system that can accurately control and display the temperature in the box to ensure that the temperature remains within the required range. The high temperature box 200 is further equipped with safety features such as overheating protection and automatic power-off functions to prevent damage caused by excessive temperature. The box door or cover is designed to be transparent so that the situation inside the box can be observed without opening the door. In order to ensure the long-term and effective operation of the high temperature box 200, regular maintenance and cleaning are required to check whether the heating elements (such as heating resistors) and the temperature control system are working properly.

[0060] Low temperature box 300: It has a second inner cavity for accommodating at least one inner bottle 110, which is used to cool the sample container 100 containing the soil sample 400 to 0℃~25℃, so that a negative pressure of 0.7~0.9atm is formed in the outer bottle 120. The box body of the low temperature box 300 is usually made of high-quality thermal insulation materials, such as polyurethane foam or polyisobutylene, to ensure the stability of the internal low temperature environment and reduce the intrusion of external heat. The low temperature box 300 is also provided with a precise temperature control system, which can adjust and maintain the temperature in the box within the range of 0℃~25℃ to meet the temperature conditions required for the preservation of the soil sample 400. The box body has a built-in refrigeration system, including a compressor, an evaporator and a condenser, which is used to reduce the temperature in the box and maintain the required low temperature environment. Preferably, a circulating air duct and a fan are also designed in the box body to ensure uniform air flow in the box, uniform temperature distribution, and avoid local overheating or overcooling. A good sealing strip is designed between the door and the box body of the low temperature box 300 to reduce the loss of cold air and the entry of external air, and ensure the stability of the negative pressure and low temperature environment in the box. The box is also designed with a user-friendly operating interface, including temperature display, setting and adjustment buttons, and possible fault diagnosis and alarm systems. Alternatively, the low temperature box 300 can be selected from a refrigerator or an incubator, and equipment with similar structures and functions.

[0061] Steps: Step 1: Preheat the sample container 100 First, the outer bottle 120 of the sample container 100 is preheated using the high temperature box 200 provided by the present invention. The outer bottle 120 is placed in the high temperature box 200 and heated to a temperature range of 50°C to 100°C, so that the air inside the bottle expands, preparing for the subsequent formation of a negative pressure environment. This step is critical because it creates conditions for the formation of negative pressure.

[0062] Step 2: Load soil sample 400 At room temperature or in an environment of 0°C to 25°C, the soil sample 400 is placed in an inner bottle 110 designed as an opaque or translucent glass container. The inner bottle 110 is preferably made of brown glass to reduce the impact of light on the sample. The outer wall of the inner bottle 110 is provided with an insulating layer 111, and a soil sample 400 information label 112 containing information such as the sample name, collection location, collection date, and sample number, for easy recording and tracing. These information labels can be made into bar codes, QR codes, or electronic tags, etc. After the sample is loaded, the inner bottle 110 is closed with an inner cover 131.

[0063] Step 3: Add soil sample 400 and close sample container 100 After the outer bottle 120 is preheated, it is taken out of the high temperature box 200, and the sealed inner bottle 110 containing the soil sample 400 is immediately placed in the outer bottle 120. Then, the outer cover 132 is tightened to ensure the sealing of the sample container 100. The outer cover 132 is designed to have an outer cover 132 and an inner cover 131 on the same surface, and a sealing ring 133 is arranged inside the outer cover 132, which enhances the sealing effect of the container and maintains the negative pressure state in the container.

[0064] Step 4: Cool down to create negative pressure and save After the sample container 100 is sealed, it is quickly placed in a cryogenic box 300 for cooling. The temperature of the cryogenic box 300 is set between 0°C and 25°C to quickly cool the sample container 100 and form a negative pressure environment of 0.7 to 0.9 atm. During the entire process, the soil sample 400 is kept away from light to protect the soil sample 400 from light and maintain its stability and integrity.

[0065] Step 5: Sampling When sampling is required, the sample container 100 stored under negative pressure is taken out from the low temperature box 300 and placed in the high temperature box 200 for reheating to eliminate the internal negative pressure.

[0066] After reheating, the air pressure in the outer bottle 120 returns to normal atmospheric pressure, so that the cover of the outer bottle 120 can be easily opened to perform the sampling operation of the soil sample 400.

[0067] The device ensures the quality and safety of 400 soil samples during long-term storage by precisely controlling temperature and creating a negative pressure environment.

[0068] The designed sealing system and negative pressure environment reduce the impact of the external environment on the soil sample 400 and improve the stability of the sample.

[0069] The device is easy to operate and improves the efficiency and reliability of soil sample 400 preservation and sampling.

[0070] In some embodiments, both the inner bottle 110 and the outer bottle 120 are glass bottles, and the preservation effect of the soil sample is optimized by selecting glass materials with different thermal conductivity coefficients.

[0071] The outer bottle 120 is made of a glass material with high thermal conductivity, which can respond to external temperature changes more quickly and quickly reach the temperature required for preheating or cooling, thereby improving overall work efficiency. Specifically, the outer bottle 120 can be made of a glass material with high thermal conductivity (about 1.3~1.5 W / m·K) such as borosilicate glass to quickly transfer heat.

[0072] Choosing a glass material with low thermal conductivity for the inner bottle helps maintain a stable temperature of the soil sample inside and reduce internal temperature fluctuations caused by external temperature changes. Specifically, the inner bottle can be made of glass materials with low thermal conductivity (about 1.0~1.2 W / m·K) such as soda-lime glass to reduce heat transfer. The low thermal conductivity of the inner bottle can act as a barrier to protect the soil sample from external temperature changes and maintain the stability and integrity of the sample.

[0073] Example 6 This embodiment is an alternative embodiment of Embodiment 5. Figure 1~Figure 5 In order to improve the temperature response speed of the air in the outer bottle, metal material is selected to make the outer bottle 120. Due to its good thermal conductivity, the metal material can respond to temperature changes more quickly, which is also important for application scenarios that require fast preheating and cooling.

[0074] The metal outer bottle can absorb and release heat faster, which means that during the preheating process in the high temperature box and the cooling process in the low temperature box, the metal outer bottle can reach the required temperature more quickly, thereby improving the efficiency of the entire preservation process or sampling process.

[0075] When choosing metal materials, commonly used metal materials include stainless steel, aluminum or copper.

[0076] Since the outer bottle 120 is made of metal, and the metal material is opaque, it is necessary to mark or label the metal outer bottle.

[0077] Since the inner bottle 110 is made of glass, the material of the outer bottle 120 does not affect the preservation properties of the soil sample 400 .

[0078] The present invention relies on the basic principle of the ideal gas law pV=nRT, and achieves efficient and standardized preservation of soil samples by accurately controlling the temperature change in the sample container 100 and then controlling the gas pressure change. This method expands the air in the bottle by preheating the outer bottle 120, and then cools it through the low temperature box 300 to form a negative pressure environment. It not only simplifies the traditional complex soil sample preservation process, but also significantly improves the sealing effect, and effectively prevents the sample from being damp, oxidized or contaminated by microorganisms. In addition, the technical solution achieves energy saving by reducing energy consumption, reduces operating costs, avoids the use of chemical reagents, reduces environmental pollution, and embodies environmental friendliness. The design of the negative pressure container increases the safety of the sample container 100, prevents unauthorized access or accidental opening, and protects the safety of samples and operators. In addition, the negative pressure environment helps to maintain the original state of the soil sample, reduce the degradation and change of the sample during the preservation process, and ensures the accuracy of subsequent analysis. Therefore, the present invention is not only based on a simple scientific principle, but also shows outstanding technical effects in practical applications, and provides an efficient, safe, economical and environmentally friendly solution for the long-term preservation of soil samples, which has wide applicability and significant social and economic value.

[0079] In the description of the present invention, it should be understood that "-" and "~" represent the range between two values, and the range includes the endpoints. For example: "AB" represents a range greater than or equal to A and less than or equal to B. "A~B" represents a range greater than or equal to A and less than or equal to B.

[0080] In the description of the present invention, the term "and / or" herein is merely a description of the association relationship of associated objects, indicating that three relationships may exist. For example, A and / or B may represent three situations: A exists alone, A and B exist at the same time, and B exists alone.

[0081] In the description of the invention, the numerical values ​​of time, temperature, ratio, mass, etc. involved may be based on actual measurements, standard parameters of equipment, simplified rounding results, or within an acceptable error range, ensuring the practicability and repeatability of the invention.

[0082] In the description of the present invention, the term "about" or "approximately" is used to express the approximate value of a numerical value or range, allowing a certain error to ensure the flexibility and practicality of the description while remaining within an acceptable error range, with the maximum error range not exceeding 10% of the corresponding numerical value or numerical range.

[0083] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A soil sample preservation method, characterized in that: The steps include: S1. Preheat the sample container. The air temperature in the sample container is 50℃~100℃; S2. Take the preheated sample container, add the soil sample, and quickly cover the container to seal the sample container; S3. Cool the sample container containing soil to 0℃~25℃, create negative pressure inside the sample container, and store it at 0℃~25℃ away from light.

2. The soil sample preservation method according to claim 1, characterized in that: It also includes the sampling steps: S4. Take the sample container stored under negative pressure, reheat it, eliminate the negative pressure inside the sample container, open the lid, and take a sample for sample testing.

3. The soil sample preservation method according to claim 1 or 2, characterized in that: The sample container comprises an outer bottle, an inner bottle and a bottle cap; an outer cap matched with the outer bottle and an inner cap matched with the inner bottle are arranged on the same surface of the bottle cap.

4. The soil sample preservation method according to claim 3, characterized in that: The soil sample is first placed in an inner bottle, which is sealed with a lid and stored at room temperature or at a low temperature of 0°C to 25°C.

5. The soil sample preservation method according to claim 3, characterized in that: The outer bottle is a transparent glass container or a metal container, the inner bottle is an opaque or translucent glass container, and the volume of the outer bottle is 10% to 40% greater than the volume of the inner bottle.

6. The soil sample preservation method according to claim 5, characterized in that: A soil sample information label is arranged on the outer wall of the inner bottle.

7. The soil sample preservation method according to claim 3, characterized in that: A sealing ring is arranged inside the outer cover.

8. The soil sample preservation method according to claim 1, characterized in that: The outer wall of the inner bottle is also provided with a heat insulation layer.

9. The soil sample preservation method according to claim 1, characterized in that: The negative pressure is 0.7~0.9atm.

10. A device for the soil sample preservation method according to any one of claims 1 to 9, characterized in that: include: The sample container comprises an outer bottle, an inner bottle and a bottle cap, wherein an outer cap matching with the outer bottle and an inner cap matching with the inner bottle are arranged on the same surface of the bottle cap; a sealing ring is arranged inside the outer cap; the inner bottle is used to hold the soil sample, and the outer bottle and the outer cap constitute a negative pressure storage assembly; A high temperature box, having a first inner cavity for accommodating at least one outer bottle, for preheating or reheating the outer bottle of the sample container, so that the air temperature in the bottle is heated to 50°C to 100°C; The low temperature box has a second inner cavity for accommodating at least one inner bottle, and is used to cool the sample container containing the soil sample to 0°C~25°C, so that a negative pressure of 0.7~0.9atm is formed in the outer bottle.

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