Biological fertilizer and application thereof

Abstract

The invention relates to a biological fertilizer, in particular to a fish protein oligopeptide capable of nourishing roots, rejuvenating and enhancing a plant immune system, which is composed of the following components in concentration: fish protein oligopeptide (llt; 3000 Da > 120 g / L, organic matter 100 g / L and free amino acid 40 g / L. The biological fertilizer provided by the invention contains rich protein molecules, polypeptides and other organic nutrient substances, and has the effects of enhancing plant stress resistance, improving the effective utilization rate of the fertilizer and improving the rhizosphere beneficial microorganism ecological environment, and the fish protein oligopeptide also has an antiviral activity function.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological fertilizers, and in particular relates to a fish protein oligopeptide biological fertilizer that can nourish roots and strengthen plants and increase the immune system of plants. Background Art

[0002] Due to the long-term and large-scale use of chemical fertilizers, the soil structure, nutrient balance and microbial diversity have been seriously damaged, resulting in poor tobacco growth, increased diseases and pests, difficulty in increasing yields, and reduced tobacco leaf quality.

[0003] The enhancement of health and environmental awareness and the improvement of living standards have led to changes in the agricultural product consumption market, and people's demand for green food is growing. In order to make agricultural products meet the requirements of green safety, it is necessary to change the original unreasonable fertilization methods. Biofertilizer is an effective way to change the drawbacks of the current use of chemical fertilizers. Summary of the invention

[0004] The purpose of the present invention is to address the deficiencies of the prior art and provide a fish protein oligopeptide that can provide more nutrients while having the advantages of biological fertilizers, and has the capabilities of improving soil structure, improving the ecological environment of beneficial microorganisms in the rhizosphere, ensuring the sustainability of the tobacco field ecosystem, inducing tobacco to produce resistance, regulating physiological functions to improve immunity, regulating crop respiration, and improving tobacco leaf quality.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] A biological fertilizer comprises three components, namely, fish protein oligopeptide, organic matter and free amino acid, wherein the concentration of each component is 120 g / L of fish protein oligopeptide, 100 g / L of organic matter and 40 g / L of free amino acid.

[0007] Preferably, as a preferred embodiment, the molecular weight of the fish protein oligopeptide is less than 3000Da.

[0008] Another object of the present invention is to provide the use of the above-mentioned biological fertilizer for improving tobacco stress resistance and preventing and treating tobacco virus diseases.

[0009] Preferably, as a preferred embodiment, the tobacco virus disease is caused by tobacco mosaic virus (TMV).

[0010] Preferably, as a preferred embodiment, the control effect of the biofertilizer on TMV is achieved by foliar spraying.

[0011] Preferably, as a better embodiment, the foliar spraying concentration is diluted 1000 times, with an interval of 7-10 days per time, and it is best to apply it twice in a row.

[0012] Another object of the present invention is to provide the use of the biological fertilizer in improving tobacco yield and tobacco leaf quality.

[0013] Preferably, as a preferred embodiment, the application of the biofertilizer to improve tobacco yield and tobacco leaf quality is achieved through root irrigation.

[0014] Preferably, as a preferred embodiment, the root irrigation of the biological fertilizer is best diluted 500-800 times, and the specific amount can be appropriately adjusted according to the actual fertility of the soil and the fertilizer application of tobacco.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The fish protein oligopeptide contained in the present invention is a small molecule protein, which is more easily absorbed by plants, has a significantly higher antiviral effect than fish protein, and has an antagonistic effect on TMV. After adding organic matter and free amino acids, its rich protein molecules and organic nutrients such as polypeptides can enhance the stress resistance of plants, and it has the advantages of biological fertilizers while providing more nutrient elements. It can improve soil structure, loosen soil, improve air permeability, and improve fertilizer and water retention capacity, effectively promote the decomposition and release of insoluble and solidified nutrients in the soil, increase the supply of effective nutrients in the soil, and improve the effective utilization rate of fertilizers. It has good market application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0018] Figure 1 The effects of different treatments on the amount of viral expression are shown;

[0019] Figure 2 The effects of different treatments on tobacco diseases are shown. DETAILED DESCRIPTION

[0020] The present invention will now be further described in detail in conjunction with the accompanying drawings and embodiments. These drawings are simplified schematic diagrams that only illustrate the basic concept of the present invention in a schematic manner, and therefore only show the structures related to the present invention.

[0021] Unless otherwise specified, the reagents and materials used in the examples of the present invention are commercially available, wherein the chemical reagents and organic materials used are purchased from Qingdao Keshang Biotechnology Co., Ltd., fish protein is purchased from Ningbo Jifeng Biotechnology Development Co., Ltd., and fish protein oligopeptides are purchased from Ningbo Zhouyu Marine Technology Co., Ltd.

[0022] 1. Specific Example A biological fertilizer comprises 3 components, wherein the concentration of each component is: fish protein oligopeptide (<3000Da) 120g / L, organic matter 100g / L, free amino acid 40g / L.

[0024] 1. Embodiment 1

[0025] A biological fertilizer comprises 3 parts, and the formula comprises three parts in total. The formula components are as follows: 120g / L fish protein, 100g / L organic matter, and 40g / L free amino acids.

[0026] 2. Embodiment 2

[0027] A biological fertilizer has the following formula: its components include 3 parts, and the formula has three parts in total, and the formula components are as follows: 120g / L fish protein oligopeptide, 100g / L organic matter, and 40g / L free amino acids.

[0028] 3. Comparative Example 1

[0029] The clear water treatment was used as blank control.

[0030] 2. Inhibitory effects of different treatments on tobacco virus diseases

[0031] Take 0.5g of fresh infected TMV leaves and grind them to homogenate, add 20mL inoculation buffer, take the supernatant after centrifugation, and prepare 40 times of virus juice. The biofertilizer of the present invention and the virus juice are mixed in equal volumes for 10min, and the treatment of mixing the same amount of clean water with the virus juice is used as the positive control. By conventional friction inoculation, select Sanshengyan-NN with consistent growth at the 4-5 leaf stage, adopt the half-leaf method, the right half leaf is the blank control, and the left half leaf is the treatment. Each treatment has 4-5 leaves, and it is repeated 3 times. The results showed that in the anti-TMV test, the number of TMV necrotic spots in the right half of the leaf in the fish protein treatment group was 100-120, and the number of necrotic spots in the left half of the leaf was only 40-50, and the TMV inhibition rate of fish protein treatment was more than 58%; in the fish protein oligopeptide treatment group, the number of TMV necrotic spots in the right half of the leaf was 90-110, and the number of necrotic spots in the left half of the leaf was only 6-8. The TMV inhibition rate of fish protein oligopeptide reached more than 93%, with significant inhibitory activity, which was 60.3% higher than that of the fish protein treatment group.

[0032] 3. Determination of antiviral activity of different treatments

[0033] After grinding and homogenizing fresh virus source leaves, the virus was inoculated by rubbing on Nicotiana benthamiana leaves. After 24 hours, 10 mL of 120 g / L fish protein and fish protein oligopeptide were sprayed respectively, with conventional antiviral agent Ningnanmycin as the control and an equal amount of deionized water as the blank control. After 72 hours, the total RNA of the system leaves was extracted, and the qRT-PCR virus amount was detected to determine the antiviral activity of different treatments. The results showed that after inoculation with TMV, the virus content of the fish protein oligopeptide treatment group was significantly lower than that of the fish protein and Ningnanmycin treatment groups, and the relative prevention effect reached 76.4%, which was 21.1% and 27.8% higher than that of the fish protein treatment group (63.1%) and Ningnanmycin group (59.8%), respectively. This shows that fish protein oligopeptide has a strong inhibitory effect on the infection and replication of TMV, and spraying fish protein oligopeptide has a good prevention and control potential for tobacco virus diseases.

[0034] 4. Effects of different treatments on tobacco leaf yield

[0035] In 2023, a field experiment was carried out in Weizhou Town, Zhangjiakou City, Hebei Province. The local conventional fertilization was used as the control, and fish protein oligopeptide biofertilizer was used as treatment 1 (T1). The biofertilizer ratio was: fish protein oligopeptide 120g / L, organic matter 100g / L, free amino acid 40g / L, 3-5kg / 667m2 root irrigation at the seedling stage, 5-10kg / 667m2 at the cluster stage. 2 Root irrigation once; fish protein biofertilizer was used as treatment 2 (T2), with the ratio of fish protein 120g / L, organic matter 100g / L, free amino acid 40g / L, 3-5kg / 667m at the seedling stage 2 Root irrigation once, 5-10kg / 667m during the clustering period 2Root irrigation once; the control and treatment used the single factor same field comparison method, only different in fertilizer use (CK), all other aspects were consistent, no repetition. The row spacing, plant spacing and planting density of tobacco in the experimental field were consistent with the local flue-cured tobacco field production. After 30 days of field test, the agronomic traits such as plant height, stem girth, maximum leaf length, maximum leaf width and number of effective leaves of the treatment and control flue-cured tobacco were investigated by a 5-point survey method in accordance with the YC / T 142-2002 industry standard, with 30 plants at each point. The proportion of medium and high-quality tobacco in each treatment was calculated. The results showed that the agronomic traits of flue-cured tobacco treated with biofertilizer containing fish protein oligopeptides were improved to varying degrees at different growth stages compared with those of fish protein biofertilizer and the control group. At the capping stage, plant height, stem girth, number of effective leaves, maximum leaf width and maximum leaf length increased by 5.61%, 8.42%, 1.66%, 2.62% and 1.92% respectively compared with those of the fish protein biofertilizer group, and increased by 11.26%, 13.19%, 3.37%, 3.83% and 6.48% respectively compared with those of the control group, among which plant height and stem girth increased most significantly. At the same time, the proportion of high-quality tobacco also increased significantly, increasing by 9.02% compared with the fish protein biofertilizer group and 15.4% compared with the control group. The yield and output value were both improved, mainly by increasing the proportion of high-quality tobacco to increase the average price and thus increase the output value.

[0036] Table 1 Effects of biofertilizers on agronomic traits of flue-cured tobacco

[0037]

[0038] Table 2 Effects of biofertilizer on economic characteristics of flue-cured tobacco

[0039]

[0040] The above is based on the ideal embodiment of the present invention. Through the above description, relevant personnel can make various changes and modifications without departing from the technical concept of the present invention. The technical scope of the present invention is not limited to the content in the specification, and the technical scope must be determined according to the scope of the claims.

Claims

1. A biological fertilizer, characterized in that: It includes three components: fish protein oligopeptides, organic matter and free amino acids.

2. A biological fertilizer according to claim 1, characterized in that: The molecular weight of the fish protein oligopeptide is less than 3000Da.

3. A biological fertilizer according to claim 1, characterized in that: It contains 3 components, and the concentration of each component is 120g / L fish protein oligopeptide, 100g / L organic matter, and 40g / L free amino acid.

4. Use of the biofertilizer according to claim 3 for improving tobacco stress resistance and preventing and treating tobacco virus diseases.

5. The use according to claim 4, characterized in that: The control effect of the biological fertilizer on TMV is achieved by foliar spraying.

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