Soybean protein hydrogel containing beta-nicotinamide mononucleotide as well as preparation method and application of soybean protein hydrogel

Abstract

The invention particularly discloses soybean protein hydrogel containing beta-nicotinamide mononucleotide as well as a preparation method and application of the soybean protein hydrogel. The preparation method of the soybean protein hydrogel containing beta-nicotinamide mononucleotide comprises the following steps: adding soybean protein into an alkaline solution A, and uniformly stirring to obtain a dispersion solution A; adding chitosan and beta-nicotinamide mononucleotide into an acetic acid solution, and uniformly stirring to obtain dispersion liquid B; the dispersion liquid A and the dispersion liquid B are mixed, then an alkaline solution B is added, and standing is conducted for 10-30 min after the alkaline solution B is added; and separating the hydrogel, and washing to obtain the soybean protein hydrogel containing the beta-nicotinamide mononucleotide. According to the invention, beta-nicotinamide mononucleotide is loaded in soybean protein hydrogel; the premature degradation of the beta-nicotinamide mononucleotide in the digestive tract is avoided; the important application value is realized. In addition, the soybean protein hydrogel containing the beta-nicotinamide mononucleotide disclosed by the invention also has an effect of reducing blood sugar.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a soybean protein hydrogel containing beta-nicotinamide mononucleotide, and a preparation method and application thereof. Background Art

[0002] β-Nicotinamide mononucleotide, also known as nicotinamide mononucleotide, abbreviated as NMN, is a naturally occurring biologically active nucleotide. It is an inherent substance in the human body, widely involved in many biochemical reactions in the human body, and is closely related to immunity and metabolism. Therefore, it has attracted much attention in the field of health foods.

[0003] However, β-nicotinamide mononucleotide is degraded after entering the oral cavity, making it difficult to reach the small intestine, resulting in low bioavailability; a new dosage form that can sustain the release of β-nicotinamide mononucleotide needs to be developed so that β-nicotinamide mononucleotide can be released and absorbed after reaching the small intestine. Chinese invention patent 201610378420.2 discloses a soybean protein composite chitosan spherical hydrogel, which uses anionic natural polymer soybean protein and cationic natural polymer chitosan as raw materials to prepare soybean protein composite chitosan spherical hydrogel. The spherical hydrogel has obvious pH responsiveness, ionic strength responsiveness and biodegradability, and has good swelling properties. Drug loading and release experiments show that the hydrogel has good sustained release performance for small molecule drugs and a high cumulative release rate, so it has obvious application prospects in the field of oral enteric small molecule drug carriers.

[0004] However, there is no research report on using soybean protein hydrogel as a carrier of β-nicotinamide mononucleotide in the prior art; therefore, improving a soybean protein hydrogel containing β-nicotinamide mononucleotide has important application value. Summary of the invention

[0005] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a soybean protein hydrogel containing β-nicotinamide mononucleotide and a preparation method and application thereof.

[0006] The above technical problems to be solved by the present invention are achieved through the following technical solutions:

[0007] The present invention first provides a method for preparing a soybean protein hydrogel containing β-nicotinamide mononucleotide, which comprises the following steps:

[0008] Add soybean protein to alkaline solution A and stir evenly to obtain dispersion A;

[0009] Add chitosan and β-nicotinamide mononucleotide to the acetic acid solution and stir evenly to obtain dispersion B;

[0010] The dispersion A and the dispersion B are mixed, and then the alkaline solution B is added. After the alkaline solution B is added, the mixture is allowed to stand for 10 to 30 minutes. The hydrogel is separated and washed to obtain the soybean protein hydrogel containing β-nicotinamide mononucleotide.

[0011] The present invention provides a novel method for preparing soybean protein hydrogel containing β-nicotinamide mononucleotide; the method utilizes the principle that soybean protein hydrogel can be used as a sustained-release drug to load β-nicotinamide mononucleotide into the soybean protein hydrogel; the method avoids premature degradation of β-nicotinamide mononucleotide in the digestive tract; and the method has important application value.

[0012] Preferably, the usage ratio of soybean protein to alkaline solution A is 1 g: 10-20 mL.

[0013] Most preferably, the usage ratio of soybean protein to alkaline solution A is 1 g:15 mL.

[0014] Preferably, the alkaline solution A refers to a sodium hydroxide aqueous solution with a mass fraction of 10 to 20%.

[0015] Most preferably, the alkaline solution A refers to a sodium hydroxide aqueous solution with a mass fraction of 15%.

[0016] Preferably, the usage ratio of chitosan, β-nicotinamide mononucleotide and acetic acid solution is 5-10 g:10-20 g:50-100 mL;

[0017] Most preferably, the usage ratio of chitosan, β-nicotinamide mononucleotide and acetic acid solution is 8g:15g:80mL.

[0018] Preferably, the acetic acid solution refers to an acetic acid aqueous solution with a mass fraction of 0.5% to 2%.

[0019] Most preferably, the acetic acid solution refers to an acetic acid aqueous solution with a mass fraction of 1%.

[0020] Preferably, the volume ratio of the dispersion A, the dispersion B and the alkaline solution B is 1-2:1-3:1-2.

[0021] Most preferably, the volume ratio of the dispersion A, the dispersion B and the alkaline solution B is 1:1.5:1. Preferably, the alkaline solution B is a sodium hydroxide ethanol solution with a mass fraction of 30% to 50%.

[0022] Most preferably, the alkaline solution B is a sodium hydroxide ethanol solution with a mass fraction of 40%. Preferably, a Chinese herbal medicine extract is also added to the dispersion B;

[0023] The Chinese medicine extract is prepared by the following method:

[0024] Take the traditional Chinese medicine kudzu root and polygonatum odoratum, crush them and then extract them with a solvent, separate the extract after the extraction, concentrate and dry the extract to obtain the kudzu root-polygonatum odoratum solvent extract; take the kudzu root-polygonatum odoratum solvent extract to obtain the traditional Chinese medicine extract.

[0025] The inventors found in further research that during the preparation process of the soybean protein hydrogel containing β-nicotinamide mononucleotide, adding the kudzu root-polygonatum odoratum solvent extract prepared from the traditional Chinese medicine kudzu root and polygonatum odoratum as raw materials can make the soybean protein hydrogel containing β-nicotinamide mononucleotide also have a blood sugar lowering effect.

[0026] Preferably, the usage ratio of chitosan, β-nicotinamide mononucleotide, Chinese herbal medicine extract and acetic acid solution is 5-10 g:10-20 g:10-20 g:50-100 mL.

[0027] Most preferably, the usage ratio of chitosan, β-nicotinamide mononucleotide, Chinese herbal medicine extract and acetic acid solution is 8g:15g:15g:80mL.

[0028] Preferably, the weight ratio of the traditional Chinese medicines Pueraria root and Polygonatum odoratum is 1 to 3:1.

[0029] Most preferably, the weight ratio of the Chinese medicines Pueraria lobata and Polygonatum odoratum is 2:1.

[0030] Preferably, the total weight ratio of the traditional Chinese medicines Puerariae Radix and Polygonatum odoratum to the solvent is 1 g: 10-20 mL.

[0031] Most preferably, the total weight ratio of the traditional Chinese medicines Puerariae Radix and Polygonatum odoratum to the solvent is 1 g:15 mL.

[0032] Preferably, the solvent is an ethanol aqueous solution with a volume fraction of 60 to 80%.

[0033] Most preferably, the solvent is an ethanol aqueous solution with a volume fraction of 70%.

[0034] Preferably, the extraction refers to heating reflux extraction.

[0035] Preferably, the temperature of the heating reflux extraction is 70-80° C., and the extraction time is 1-2 hours.

[0036] Most preferably, the temperature of the heating reflux extraction is 75° C. and the extraction time is 1.5 h.

[0037] Preferably, the method for preparing the Chinese medicine extract further comprises the following steps:

[0038] The kudzu root-polygonatum odoratum solvent extract is dispersed in water, then extracted with an organic solvent extractant, the organic solvent extract is separated, concentrated and dried to obtain the traditional Chinese medicine extract.

[0039] Preferably, the ratio of the Pueraria-Polygonatum odoratum solvent extract to water and the organic solvent extractant is 1 g: 20-40 mL: 20-40 mL.

[0040] Most preferably, the ratio of the Pueraria-Polygonatum solvent extract to water and the organic solvent extractant is 1 g:30 mL:30 mL.

[0041] Preferably, the organic solvent extractant consists of tetrachloroethane and diethyl ether.

[0042] Preferably, the volume ratio of tetrachloroethane to diethyl ether is 2-4:1-3.

[0043] Most preferably, the volume ratio of tetrachloroethane to diethyl ether is 3:2.

[0044] The inventors found in further research that in the preparation process of soybean protein hydrogel containing β-nicotinamide mononucleotide, adding a traditional Chinese medicine extract extracted by an organic solvent extractant composed of tetrachloroethane and ether can further significantly improve the hypoglycemic effect of soybean protein hydrogel containing β-nicotinamide mononucleotide, compared with adding an unextracted Pueraria lobata-Polygonatum odoratum solvent extract.

[0045] The inventors found in the study that the selection of the organic solvent extractant of the present invention is very critical; in the preparation process of the soybean protein hydrogel containing β-nicotinamide mononucleotide, adding the Chinese medicine extract obtained by extraction with other organic solvents cannot further significantly improve the hypoglycemic effect of the soybean protein hydrogel containing β-nicotinamide mononucleotide; only by adding the Chinese medicine extract obtained by extraction with an organic solvent extractant composed of tetrachloroethane and ether, can the hypoglycemic effect of the soybean protein hydrogel containing β-nicotinamide mononucleotide be further significantly improved.

[0046] The present invention also provides a soybean protein hydrogel containing β-nicotinamide mononucleotide prepared by the above preparation method.

[0047] The present invention also provides an application of the soybean protein hydrogel in preparing food or medicine.

[0048] Preferably, the food or medicine is a food or medicine having anti-aging and immunity-enhancing effects.

[0049] Preferably, the food or medicine is a food or medicine having a blood sugar lowering effect.

[0050] Beneficial effects: The present invention provides a new soybean protein hydrogel containing β-nicotinamide mononucleotide. The method uses the principle that soybean protein hydrogel can be used as a sustained-release drug to load β-nicotinamide mononucleotide in the soybean protein hydrogel; it avoids the premature degradation of β-nicotinamide mononucleotide in the digestive tract; and has important application value. In addition, the present invention also adds a kudzu root-polygonatum solvent extract prepared from Chinese medicine kudzu root and polygonatum as raw materials during the preparation process of the soybean protein hydrogel containing β-nicotinamide mononucleotide, which can make the soybean protein hydrogel containing β-nicotinamide mononucleotide also have a hypoglycemic effect. DETAILED DESCRIPTION

[0051] The present invention is further explained below in conjunction with specific examples, but the examples do not limit the present invention in any form.

[0052] Example 1 Preparation of soybean protein hydrogel containing β-nicotinamide mononucleotide

[0053] (1) adding soybean protein to alkaline solution A and stirring to obtain dispersion A; wherein the ratio of soybean protein to alkaline solution A is 1 g:15 mL; and alkaline solution A refers to a sodium hydroxide aqueous solution with a mass fraction of 15%;

[0054] (2) adding chitosan and β-nicotinamide mononucleotide to an acetic acid solution and stirring to obtain a dispersion B; the amount ratio of chitosan, β-nicotinamide mononucleotide and acetic acid solution is 8 g:15 g:80 mL; the acetic acid solution refers to an acetic acid aqueous solution with a mass fraction of 1%;

[0055] (3) Dispersion A and dispersion B are mixed, and then alkaline solution B is added (dropped), and after the addition of alkaline solution B, the mixture is allowed to stand for 20 minutes; the hydrogel is separated, and after washing, the soy protein hydrogel containing β-nicotinamide mononucleotide is obtained; wherein the volume ratio of dispersion A, dispersion B and alkaline solution B is 1:1.5:1; and the alkaline solution B is a sodium hydroxide ethanol solution with a mass fraction of 40%.

[0056] Example 2 Preparation of soybean protein hydrogel containing β-nicotinamide mononucleotide

[0057] (1) adding soybean protein to alkaline solution A and stirring to obtain dispersion A; wherein the ratio of soybean protein to alkaline solution A is 1 g:15 mL; and alkaline solution A refers to a sodium hydroxide aqueous solution with a mass fraction of 15%;

[0058] (2) adding chitosan, β-nicotinamide mononucleotide and Chinese herbal medicine extract to acetic acid solution and stirring to obtain dispersion B; the amount ratio of chitosan, β-nicotinamide mononucleotide, Chinese herbal medicine extract and acetic acid solution is 8g:15g:15g:80mL; the acetic acid solution refers to an acetic acid aqueous solution with a mass fraction of 1%;

[0059] (3) mixing dispersion A and dispersion B, and then adding (dropping) alkaline solution B, and standing for 20 minutes after the addition of alkaline solution B; separating the hydrogel, and washing to obtain the soy protein hydrogel containing β-nicotinamide mononucleotide; wherein the volume ratio of dispersion A, dispersion B and alkaline solution B is 1:1.5:1; and the alkaline solution B is a sodium hydroxide ethanol solution with a mass fraction of 40%;

[0060] The Chinese medicine extract is prepared by the following method: taking Chinese medicines Pueraria root and Polygonatum odoratum, crushing them, and then heating and refluxing them with a solvent at 75°C for 1.5 hours, separating the extract after the extraction, and concentrating and drying the extract to obtain a Pueraria root-Polygonatum odoratum solvent extract; taking the Pueraria root-Polygonatum odoratum solvent extract to obtain the Chinese medicine extract;

[0061] The weight ratio of the traditional Chinese medicine Pueraria root and Polygonatum odoratum is 2:1; the total weight of the traditional Chinese medicine Pueraria root and Polygonatum odoratum and the amount of the solvent is 1g:15mL; the solvent refers to an ethanol aqueous solution with a volume fraction of 70%.

[0062] Example 3 Preparation of soybean protein hydrogel containing β-nicotinamide mononucleotide

[0063] (1) adding soybean protein to alkaline solution A and stirring to obtain dispersion A; wherein the ratio of soybean protein to alkaline solution A is 1 g:15 mL; and alkaline solution A refers to a sodium hydroxide aqueous solution with a mass fraction of 15%;

[0064] (2) adding chitosan, β-nicotinamide mononucleotide and Chinese herbal medicine extract to acetic acid solution and stirring to obtain dispersion B; the amount ratio of chitosan, β-nicotinamide mononucleotide, Chinese herbal medicine extract and acetic acid solution is 8g:15g:15g:80mL; the acetic acid solution refers to an acetic acid aqueous solution with a mass fraction of 1%;

[0065] (3) mixing dispersion A and dispersion B, and then adding (dropping) alkaline solution B, and standing for 20 minutes after the addition of alkaline solution B; separating the hydrogel, and washing to obtain the soy protein hydrogel containing β-nicotinamide mononucleotide; wherein the volume ratio of dispersion A, dispersion B and alkaline solution B is 1:1.5:1; and the alkaline solution B is a sodium hydroxide ethanol solution with a mass fraction of 40%;

[0066] The Chinese medicine extract is prepared by the following method:

[0067] (1) Taking Chinese medicine kudzu root and polygonatum odoratum, crushing them, and then heating and refluxing them with a solvent at 75° C. for 1.5 hours, separating the extract after the extraction, and concentrating and drying the extract to obtain a kudzu root-polygonatum odoratum solvent extract; wherein the weight ratio of the Chinese medicine kudzu root and polygonatum odoratum is 2:1; the total weight ratio of the Chinese medicine kudzu root and polygonatum odoratum to the solvent is 1g:15mL; the solvent refers to an ethanol aqueous solution with a volume fraction of 70%;

[0068] (2) dispersing the Pueraria root-Polygonatum odoratum solvent extract in water, then extracting with an organic solvent extractant, separating the organic solvent extract, concentrating and drying to obtain the Chinese medicine extract; wherein the amount ratio of the Pueraria root-Polygonatum odoratum solvent extract to water and the organic solvent extractant is 1g:30mL:30mL; the organic solvent extractant is composed of tetrachloroethane and ether in a volume ratio of 3:2.

[0069] Comparative Example 1 Preparation of soybean protein hydrogel containing β-nicotinamide mononucleotide

[0070] The difference between Comparative Example 1 and Example 3 is that the preparation method of the Chinese herbal medicine extract is different.

[0071] The Chinese medicine extract described in Comparative Example 1 was prepared by the following method:

[0072] (1) Taking Chinese medicine kudzu root and polygonatum odoratum, crushing them, and then heating and refluxing them with a solvent at 75° C. for 1.5 hours, separating the extract after the extraction, and concentrating and drying the extract to obtain a kudzu root-polygonatum odoratum solvent extract; wherein the weight ratio of the Chinese medicine kudzu root and polygonatum odoratum is 2:1; the total weight ratio of the Chinese medicine kudzu root and polygonatum odoratum to the solvent is 1g:15mL; the solvent refers to an ethanol aqueous solution with a volume fraction of 70%;

[0073] (2) dispersing the Pueraria root-Polygonatum odoratum solvent extract in water, then extracting with an organic solvent extractant, separating the organic solvent extract, concentrating and drying to obtain the Chinese medicine extract; wherein the amount ratio of the Pueraria root-Polygonatum odoratum solvent extract to water and the organic solvent extractant is 1g:30mL:30mL; and the organic solvent extractant is tetrachloroethane.

[0074] Comparative Example 2 Preparation of soybean protein hydrogel containing β-nicotinamide mononucleotide

[0075] The difference between Comparative Example 2 and Example 3 is that the preparation method of the Chinese herbal medicine extract is different.

[0076] The Chinese medicine extract described in Comparative Example 2 was prepared by the following method:

[0077] (1) Taking Chinese medicine kudzu root and polygonatum odoratum, crushing them, and then heating and refluxing them with a solvent at 75° C. for 1.5 hours, separating the extract after the extraction, and concentrating and drying the extract to obtain a kudzu root-polygonatum odoratum solvent extract; wherein the weight ratio of the Chinese medicine kudzu root and polygonatum odoratum is 2:1; the total weight ratio of the Chinese medicine kudzu root and polygonatum odoratum to the solvent is 1g:15mL; the solvent refers to an ethanol aqueous solution with a volume fraction of 70%;

[0078] (2) dispersing the Pueraria root-Polygonatum odoratum solvent extract in water, then extracting with an organic solvent extractant, separating the organic solvent extract, concentrating and drying to obtain the Chinese medicine extract; wherein the amount ratio of the Pueraria root-Polygonatum odoratum solvent extract to water and the organic solvent extractant is 1g:30mL:30mL; and the organic solvent extractant is ether.

[0079] Comparative Example 3 Preparation of soybean protein hydrogel containing β-nicotinamide mononucleotide

[0080] The difference between Comparative Example 3 and Example 3 is that the preparation method of the Chinese medicine extract is different.

[0081] The Chinese medicine extract described in Comparative Example 3 was prepared by the following method:

[0082] (1) Taking Chinese medicine kudzu root and polygonatum odoratum, crushing them, and then heating and refluxing them with a solvent at 75° C. for 1.5 hours, separating the extract after the extraction, and concentrating and drying the extract to obtain a kudzu root-polygonatum odoratum solvent extract; wherein the weight ratio of the Chinese medicine kudzu root and polygonatum odoratum is 2:1; the total weight ratio of the Chinese medicine kudzu root and polygonatum odoratum to the solvent is 1g:15mL; the solvent refers to an ethanol aqueous solution with a volume fraction of 70%;

[0083] (2) dispersing the Pueraria root-Polygonatum odoratum solvent extract in water, then extracting with an organic solvent extractant, separating the organic solvent extract, concentrating and drying to obtain the Chinese medicine extract; wherein the amount ratio of the Pueraria root-Polygonatum odoratum solvent extract to water and the organic solvent extractant is 1g:30mL:30mL; and the organic solvent extractant is cyclohexane.

[0084] Comparative Example 4 Preparation of Soy Protein Hydrogel Containing β-Nicotinamide Mononucleotide

[0085] The difference between Comparative Example 4 and Example 3 is that the preparation method of the Chinese herbal medicine extract is different.

[0086] The Chinese medicine extract described in Comparative Example 4 was prepared by the following method:

[0087] (1) Taking Chinese medicine kudzu root and polygonatum odoratum, crushing them, and then heating and refluxing them with a solvent at 75° C. for 1.5 hours, separating the extract after the extraction, and concentrating and drying the extract to obtain a kudzu root-polygonatum odoratum solvent extract; wherein the weight ratio of the Chinese medicine kudzu root and polygonatum odoratum is 2:1; the total weight ratio of the Chinese medicine kudzu root and polygonatum odoratum to the solvent is 1g:15mL; the solvent refers to an ethanol aqueous solution with a volume fraction of 70%;

[0088] (2) dispersing the Pueraria root-Polygonatum odoratum solvent extract in water, then extracting with an organic solvent extractant, separating the organic solvent extract, concentrating and drying to obtain the Chinese medicine extract; wherein the amount ratio of the Pueraria root-Polygonatum odoratum solvent extract to water and the organic solvent extractant is 1g:30mL:30mL; and the organic solvent extractant is ethyl acetate.

[0089] Comparative Example 5 Preparation of soybean protein hydrogel containing β-nicotinamide mononucleotide

[0090] The difference between Comparative Example 5 and Example 3 is that the preparation method of the Chinese herbal medicine extract is different.

[0091] The Chinese medicine extract described in Comparative Example 5 was prepared by the following method:

[0092] (1) Taking Chinese medicine kudzu root and polygonatum odoratum, crushing them, and then heating and refluxing them with a solvent at 75° C. for 1.5 hours, separating the extract after the extraction, and concentrating and drying the extract to obtain a kudzu root-polygonatum odoratum solvent extract; wherein the weight ratio of the Chinese medicine kudzu root and polygonatum odoratum is 2:1; the total weight ratio of the Chinese medicine kudzu root and polygonatum odoratum to the solvent is 1g:15mL; the solvent refers to an ethanol aqueous solution with a volume fraction of 70%;

[0093] (2) dispersing the Pueraria root-Polygonatum odoratum solvent extract in water, then extracting with an organic solvent extractant, separating the organic solvent extract, concentrating and drying to obtain the Chinese medicine extract; wherein the amount ratio of the Pueraria root-Polygonatum odoratum solvent extract to water and the organic solvent extractant is 1g:30mL:30mL; the organic solvent extractant is composed of cyclohexane and ethyl acetate in a volume ratio of 3:2.

[0094] Experimental Example 1

[0095] Kunming mice weighing 20±2g were taken and fed with a high-sugar and high-fat diet for 1 month; then STZ solution prepared with citric acid-sodium citrate buffer with a pH of 4.5 was intraperitoneally injected at a rate of 20mg / kg twice a day; after continuing to feed with a high-sugar and high-fat diet for 1 month, mice with fasting blood glucose values ​​≥11.1mmol / L were selected as diabetic model mice.

[0096] 80 diabetic model mice were randomly divided into 8 groups, namely, a model group and experimental groups 1 to 7; in addition, 10 normal mice were taken as a normal group; the experimental groups 1 to 7 were intragastrically gavaged with the soybean protein hydrogel containing β-nicotinamide mononucleotide prepared in Examples 2 to 3 or Comparative Examples 1 to 5 at a gavage dose of 500 mg / (kg·d) per day; the model group and the normal group were gavaged with an equal amount of physiological saline; after continuous gavage for 1 month, the fasting blood glucose levels of the mice in each group were tested; the test results are shown in Table 1.

[0097] Table 1. Results of hypoglycemic experiments

[0098]

[0099]

[0100] It can be seen from the experimental results in Table 1 that the soybean protein hydrogel containing β-nicotinamide mononucleotide in Example 2 can significantly reduce the blood sugar level of diabetic mice; this shows that: in the preparation process of the soybean protein hydrogel containing β-nicotinamide mononucleotide, the addition of the kudzu root-polygonatum odoratum solvent extract prepared from the traditional Chinese medicine kudzu root and polygonatum odoratum as raw materials can make the soybean protein hydrogel containing β-nicotinamide mononucleotide have a blood sugar lowering effect.

[0101] From the experimental results in Table 1, it can be seen that the blood glucose values ​​of mice in Experimental Group 2 are significantly lower than those of mice in Experimental Group 1. This indicates that, in the preparation process of soybean protein hydrogel containing β-nicotinamide mononucleotide, the addition of a Chinese medicine extract extracted with an organic solvent extractant composed of tetrachloroethane and ether can further significantly enhance the hypoglycemic effect of soybean protein hydrogel containing β-nicotinamide mononucleotide, compared with the addition of an unextracted Pueraria-Polygonatum solvent extract.

[0102] It can be seen from the experimental results in Table 1 that, compared with the experimental group 1, the blood glucose levels of the mice in the experimental groups 3 and 4 were lower, but the reduction was not large, and the reduction was much smaller than that in the experimental group 2; this indicates that: in the preparation process of the soybean protein hydrogel containing β-nicotinamide mononucleotide, the addition of the Chinese herbal extract obtained by extraction with an organic solvent extractant consisting of tetrachloroethane and ether can significantly improve the hypoglycemic effect of the soybean protein hydrogel containing β-nicotinamide mononucleotide than the addition of the Chinese herbal extract obtained by extraction with an organic solvent extractant consisting of tetrachloroethane alone or ether alone; in the preparation process of the soybean protein hydrogel containing β-nicotinamide mononucleotide, the addition of the Chinese herbal extract obtained by extraction with an organic solvent extractant consisting of tetrachloroethane and ether can synergistically improve the hypoglycemic effect of the soybean protein hydrogel containing β-nicotinamide mononucleotide.

[0103] It can be seen from the experimental results in Table 1 that, although the blood glucose levels of mice in experimental groups 5 to 7 were lower than those in experimental group 1, the reduction was not large and was much smaller than that in experimental group 2. This indicates that the selection of the organic solvent extractant of the present invention is very critical. In the preparation process of the soybean protein hydrogel containing β-nicotinamide mononucleotide, the addition of the Chinese herbal extract obtained by extraction with other organic solvents cannot further significantly improve the hypoglycemic effect of the soybean protein hydrogel containing β-nicotinamide mononucleotide. Only by adding the Chinese herbal extract obtained by extraction with an organic solvent extractant composed of tetrachloroethane and ether can the hypoglycemic effect of the soybean protein hydrogel containing β-nicotinamide mononucleotide be further significantly improved.

Claims

1. A method for preparing a soybean protein hydrogel containing β-nicotinamide mononucleotide, characterized in that: The following steps are included: Add soybean protein to alkaline solution A and stir evenly to obtain dispersion A; Chitosan and β-nicotinamide mononucleotide were added to the acetic acid solution and stirred evenly to obtain dispersion B; The dispersion A and the dispersion B are mixed, and then the alkaline solution B is added. After the alkaline solution B is added, the mixture is allowed to stand for 10 to 30 minutes. The hydrogel is separated and washed to obtain the soybean protein hydrogel containing β-nicotinamide mononucleotide.

2. The method for preparing the soybean protein hydrogel containing β-nicotinamide mononucleotide according to claim 1, characterized in that: The dosage ratio of soybean protein to alkaline solution A is 1 g: 10-20 mL; Most preferably, the usage ratio of soybean protein to alkaline solution A is 1 g:15 mL.

3. The method for preparing the soybean protein hydrogel containing β-nicotinamide mononucleotide according to claim 1, characterized in that: Alkaline solution A refers to a sodium hydroxide aqueous solution with a mass fraction of 10 to 20%; Most preferably, the alkaline solution A refers to a sodium hydroxide aqueous solution with a mass fraction of 15%.

4. The method for preparing the soybean protein hydrogel containing β-nicotinamide mononucleotide according to claim 1, characterized in that: The dosage ratio of chitosan, β-nicotinamide mononucleotide and acetic acid solution is 5-10 g:10-20 g:50-100 mL; Most preferably, the usage ratio of chitosan, β-nicotinamide mononucleotide and acetic acid solution is 8g:15g:80mL.

5. The method for preparing the soybean protein hydrogel containing β-nicotinamide mononucleotide according to claim 1, characterized in that: The acetic acid solution refers to an acetic acid aqueous solution with a mass fraction of 0.5% to 2%; Most preferably, the acetic acid solution refers to an acetic acid aqueous solution with a mass fraction of 1%.

6. The method for preparing the soybean protein hydrogel containing β-nicotinamide mononucleotide according to claim 1, characterized in that: The volume ratio of the dispersion A, the dispersion B mixture and the alkaline solution B is 1-2:1-3:1-2. Most preferably, the volume ratio of the dispersion A, the dispersion B mixture and the alkaline solution B is 1:1.5:

1.

7. The method for preparing the soybean protein hydrogel containing β-nicotinamide mononucleotide according to claim 1, characterized in that: The alkaline solution B is a sodium hydroxide ethanol solution with a mass fraction of 30% to 50%; Most preferably, the alkaline solution B is a sodium hydroxide ethanol solution with a mass fraction of 40%.

8. The method for preparing the soybean protein hydrogel containing β-nicotinamide mononucleotide according to claim 1, characterized in that: A Chinese herbal medicine extract is also added to dispersion B; The Chinese medicine extract is prepared by the following method: Take Chinese medicine kudzu root and polygonatum odoratum, crush them and then extract them with a solvent, separate the extract after the extraction, concentrate and dry the extract to obtain kudzu root-polygonatum odoratum solvent extract; take the kudzu root-polygonatum odoratum solvent extract to obtain the Chinese medicine extract.

9. The soybean protein hydrogel containing β-nicotinamide mononucleotide prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the soybean protein hydrogel according to claim 9 in preparing food or medicine.

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