PCR amplification device

Abstract

The invention relates to the technical field of DNA detection, and discloses a PCR amplification device which comprises a temperature control box and a heat preservation mechanism, a heating cavity is formed in the upper end of the temperature control box, a sample supporting plate is arranged at the top of the heating cavity, and a high-temperature chamber and a low-temperature chamber are arranged on the side, close to the bottom of the temperature control box, of the heating cavity; the high-temperature chamber is communicated with the heating cavity through a first water outlet formed in the bottom of the heating cavity, the low-temperature chamber is communicated with the heating cavity through a second water outlet formed in the bottom of the heating cavity, a first switch mechanism for controlling the first water outlet to be opened and closed is arranged on the temperature control box, and a second switch mechanism for controlling the second water outlet to be opened and closed is arranged on the temperature control box; the high-temperature chamber is communicated with the heating cavity through a first water pump, the low-temperature chamber is communicated with the heating cavity through a second water pump, the heating cavity is communicated with an air cooling mechanism, and the air cooling mechanism is used for pumping external air into the heating cavity to cool the sample tube. The method has the effect of improving the PCR amplification efficiency.

Description

Technical Field

[0001] The present application relates to the field of DNA detection, and in particular to a PCR amplification device. Background Art

[0002] PCR amplifier is an instrument that uses polymerase chain reaction technology to amplify specific DNA. The principle of PCR technology is similar to the natural replication process of DNA. Its specificity depends on the complementary oligonucleotide primers at both ends of the target sequence and consists of three basic reaction steps: denaturation-annealing-extension.

[0003] After the template DNA is heated to about 93°C for a certain period of time, the double-stranded DNA of the template DNA or the double-stranded DNA formed by PCR amplification is dissociated and becomes a single strand so that it can bind to the primer and prepare for the next round of reaction.

[0004] After the template DNA is heated and denatured into a single strand, the temperature is lowered to about 55°C, and the primers are paired and bound to the complementary sequence of the template DNA single strand.

[0005] Under the action of TaqDNA polymerase, the DNA template-primer complex uses dNTP as the reaction raw material and the target sequence as the template to synthesize a new semiconservative replication chain that is complementary to the template DNA chain according to the principles of base pairing and semiconservative replication.

[0006] By repeating the three processes of denaturation-annealing-extension, more "semi-conservative replication chains" can be obtained, and this new chain can become the template for the next cycle. PCR amplification instruments can amplify specific gene fragments efficiently and quickly.

[0007] With the continuous development of transgenic technology, more and more transgenic seeds are used in agricultural production. In the seed breeding process, researchers can use PCR amplification instruments to amplify target genes, and then use gene sequencing, gene structure analysis and other means to quickly and accurately detect whether the seeds contain transgenic ingredients, as well as the specific type and sequence of the transgenic. This helps to ensure the safety and effectiveness of transgenic seeds and provide strong protection for agricultural production.

[0008] At present, in order to perform the above three steps, the PCR reactants need to be provided with corresponding temperatures and maintained for a certain period of time. Generally, the chip storing the reactants is placed in warm water, and the temperature is changed and maintained by heating the water.

[0009] With respect to the above-mentioned related technologies, the inventors believe that the existing water bath PCR amplification instrument changes the water from one reaction temperature to another by heating or cooling the water. It takes too long to simply heat the liquid to change the water from one reaction temperature to another, and during the annealing process, the water is only cooled to the annealing temperature, and the template DNA cools down slowly at the annealing temperature, resulting in low PCR amplification efficiency. Summary of the invention

[0010] In order to alleviate the problem of low PCR amplification efficiency, the present application provides a PCR amplification device.

[0011] The present application provides a PCR amplification device, which adopts the following technical solution: A PCR amplification device comprises a temperature control box and a heat preservation mechanism, wherein a heating chamber is provided at the upper end of the temperature control box, a sample support plate is provided at the top of the heating chamber, the sample support plate is connected to the temperature control box, and the sample support plate is used to fix the sample tube, a high-temperature chamber and a low-temperature chamber are provided on one side of the heating chamber close to the bottom of the temperature control box, the high-temperature chamber is used to heat the water therein to the temperature required for DNA denaturation, and the low-temperature chamber is used to heat the water therein to the temperature required for DNA annealing, the high-temperature chamber is connected to the heating chamber through a first water outlet provided at the bottom of the heating chamber, and the low-temperature chamber The heating chamber is connected with the heating chamber through a second water outlet provided at the bottom of the heating chamber, a first switch mechanism for controlling the opening and closing of the first water outlet is provided on the temperature control box, and a second switch mechanism for controlling the opening and closing of the second water outlet is provided on the temperature control box. The high-temperature chamber is connected with the heating chamber through a first water pump, and the low-temperature chamber is connected with the heating chamber through a second water pump. The heating chamber is connected with an air cooling mechanism, and the air cooling mechanism is used to draw external air into the heating chamber to cool the sample tube. The heat preservation mechanism is provided at the upper end of the temperature control box, and the heat preservation mechanism is used to separate the space above the temperature control box from the external space.

[0012] By adopting the above technical solution, a high-temperature chamber and a low-temperature chamber are provided on one side of the heating chamber close to the bottom of the temperature control box. The high-temperature chamber heats the water therein to the temperature required for DNA denaturation, and the low-temperature chamber heats the water therein to the temperature required for DNA annealing. After the sample tube is loaded onto the sample support plate, the water in the high-temperature chamber is pumped into the heating chamber by a first water pump, thereby heating the DNA in the sample tube. After heating for a specified time, the first switch mechanism controls the first water outlet to open, thereby allowing the water in the heating chamber to flow back into the high-temperature chamber through the first water outlet. The first switch mechanism controls the first water outlet to close, and the wind is used to heat the DNA in the sample tube. The cooling mechanism draws external air into the heating chamber to cool the sample tube. After the sample tube is cooled to the annealing temperature, the second water pump is used to pump the water in the low-temperature chamber into the heating chamber, so that the sample tube maintains the annealing temperature. After a certain period of time, the second switch mechanism is used to control the second water outlet to open, so that the water in the heating chamber flows back into the low-temperature chamber through the second water outlet. The above process is repeated according to the replication requirements. The heat loss is accelerated by air cooling, and the water temperature in the heating chamber is changed in coordination with the high-temperature chamber and the low-temperature chamber, which helps to quickly reach and maintain the required temperature conditions during the PCR amplification process and improve the amplification efficiency and accuracy.

[0013] Optionally, the air cooling mechanism includes a negative pressure fan, which is fixedly connected to the outer wall of the temperature control box, and an air inlet and an air outlet are respectively provided on two opposite side walls of the temperature control box, one end of the air inlet is connected to the heating chamber, and the other end of the air inlet is provided on the outer wall of the temperature control box, the air inlet is arranged near the top of the heating chamber, one end of the air outlet is connected to the heating chamber, and the other end of the air outlet is connected to the air inlet of the negative pressure fan, the air outlet is arranged near the top of the heating chamber, a heating tube is provided in the heating chamber, a temperature detection sensor is provided in the heating chamber, and the temperature detection sensor is electrically connected to the heating tube.

[0014] By adopting the above technical scheme, an air inlet and an air outlet are respectively opened on two opposite side walls of the temperature control box, and a negative pressure fan is connected to the air outlet. The negative pressure fan is used to generate negative pressure to extract the hot air in the heating chamber to achieve rapid heat dissipation; the air inlet and the air outlet are both arranged near the top of the heating chamber, so that the hot air can be more efficiently extracted from the high temperature area and discharged out of the temperature control box through the negative pressure fan. This design helps to reduce the temperature gradient and ensure that the temperature in the heating chamber is more uniform; in addition, the setting of the air inlet and the air outlet is likely to cause changes in the water temperature in the heating chamber. A heating tube is arranged in the heating chamber, and the water temperature in the heating chamber is detected by a temperature detection sensor. The temperature detection sensor is electrically connected to the heating tube. The heating tube heats the water in the heating chamber according to the data detected by the temperature detection sensor, so that the water in the heating chamber is maintained at the set temperature, thereby improving the amplification effect of the PCR device.

[0015] Optionally, there are multiple first water outlets, the first switch mechanism includes a first electric push rod and a first switch plate, the first switch plate is provided with multiple first water holes, the multiple first water holes are arranged in a one-to-one correspondence with the multiple first water outlets, the first electric push rod is fixedly connected to the outer wall of the temperature control box, the piston rod of the first electric push rod passes through the outer wall of the temperature control box and is fixedly connected to the first switch plate, the first electric push rod drives the first switch plate to slide in a horizontal direction, and the first water holes on the first switch plate can be connected to the first water outlet under the drive of the first electric push rod.

[0016] By adopting the above technical solution, a first switch plate is arranged on the top of the high-temperature chamber, and a plurality of first water holes are opened on the first switch plate. The plurality of first water holes and the plurality of first water outlets are arranged in one-to-one correspondence. The first switch plate is driven to slide in the horizontal direction by the first electric push rod, so that the connection state between the first water holes and the first water outlets on the first switch plate can be accurately controlled, thereby controlling the water in the heating chamber to flow into the high-temperature chamber.

[0017] Optionally, there are multiple second water outlets, the second switch mechanism includes a second electric push rod and a second switch plate, the second switch plate is provided with multiple second water holes, the multiple second water holes are arranged in a one-to-one correspondence with the multiple second water outlets, the second electric push rod is fixedly connected to the outer wall of the temperature control box, the piston rod of the second electric push rod passes through the outer wall of the temperature control box and is fixedly connected to the second switch plate, the second electric push rod drives the second switch plate to slide in a horizontal direction, and the second water holes on the second switch plate can be connected to the second water outlet under the drive of the second electric push rod.

[0018] By adopting the above technical solution, a second switch plate is arranged on the top of the low-temperature chamber, and a plurality of second water holes are opened on the second switch plate. The plurality of second water holes and the plurality of second water outlets are arranged in one-to-one correspondence. The second switch plate is driven to slide in the horizontal direction by the second electric push rod, and the connection state between the second water holes and the second water outlets on the second switch plate can be accurately controlled, thereby controlling the water in the heating chamber to flow into the low-temperature chamber.

[0019] Optionally, the sample support plate is provided with a plurality of loading holes for allowing sample tubes to pass through, and a plurality of rubber rings are fixedly connected to a side of the sample support plate away from the heating chamber. The plurality of rubber rings are arranged in one-to-one correspondence with the plurality of loading holes, the rubber rings are coaxially arranged with the corresponding loading holes, the inner diameter of the rubber rings is smaller than the inner diameter of the loading holes, and the sample tubes are interference connected with the rubber rings.

[0020] By adopting the above technical solution, a plurality of rubber rings are fixedly connected to the side of the sample support plate away from the heating chamber, and the plurality of rubber rings are arranged in one-to-one correspondence with the plurality of loading holes. When the sample tube is loaded onto the sample support plate, the rubber ring first passes through the rubber ring and then passes through the loading hole. The friction between the inner wall of the rubber ring and the outer wall of the sample tube is used to limit the sample tube, thereby reducing the possibility of the sample tube floating when heated, thereby improving the stability of heating the sample tube.

[0021] Optionally, the insulation mechanism includes an upper cover and a cover plate, the upper cover is hinged to the temperature control box, the upper cover is used to separate the space above the temperature control box from the external space, the cover plate is connected to the inner side of the temperature control box, and the upper cover is used to close the tube mouth of the sample tube.

[0022] By adopting the above technical solution, a cover plate is arranged on the inner side of the upper cover, and the cover plate is used to seal the tube mouth of the sample tube, thereby reducing the heat loss of the sample tube at the tube mouth, thereby improving the heating efficiency of the sample tube.

[0023] Optionally, the cover plate is elastically connected to the upper cover via a spring, a plurality of temperature detection probes are arranged on the cover plate, the plurality of temperature detection probes are arranged in one-to-one correspondence with a plurality of sample tubes, the temperature detection probes are used to detect the temperature in the corresponding sample tubes, a controller is arranged on the outside of the cover plate, the plurality of temperature detection probes are electrically connected to the controller, and the temperature detection sensor, the first water pump, the second water pump, the first electric push rod, the second electric push rod and the heating tube are electrically connected to the controller.

[0024] By adopting the above technical solution, multiple temperature detection probes are set on the cover plate, and the temperature in the sample tube is detected by the temperature detection probes. The temperature detection probes are electrically connected to the controller, which controls the first water pump, the second water pump, the first electric push rod and the second electric push rod to work according to the data detected by the temperature detection probes.

[0025] Optionally, an electromagnet is fixedly connected to the inner side of the cover plate, a ferromagnetic metal block is correspondingly provided on the side of the upper cover close to the cover plate, a first guide column is fixedly connected to the inner side of the cover plate, a second guide column is fixedly connected to the side of the upper cover close to the cover plate, a connecting hole is provided at the end of the first guide column away from the cover plate, and an end of the second guide column away from the cover plate is inserted into the connecting hole, a trigger switch is provided at the upper end of the temperature control box, the trigger switch is used to detect whether the upper cover is closed on the top of the temperature control box, and the trigger switch is electrically connected to the electromagnet.

[0026] By adopting the above technical scheme, an electromagnet is fixedly connected to the inner side of the cover plate, and a ferromagnetic metal block is correspondingly arranged on the side of the upper cover close to the cover plate. Before the cover plate contacts the trigger switch, the electromagnet is used to adsorb the ferromagnetic metal block, so that the upper cover moves in the direction close to the cover plate, thereby reducing the influence of the temperature detection probe on the closing of the upper cover. When the upper cover is closed on the temperature control box, the upper cover contacts the trigger switch, and the trigger switch controls the electromagnet to cut off the power, and the cover plate moves in the direction away from the upper cover under the elastic force of the spring. During the movement of the cover plate, the temperature detection probe is inserted into the corresponding sample tube, and the sample tube is closed by the cover plate at the same time. The movement of the cover plate is guided by the cooperation of the first guide column and the second guide column, thereby improving the stability of the movement of the cover plate. In addition, the sample tube is protected by a rubber ring arranged on the sample support plate, thereby reducing the possibility of the sample tube being squeezed and damaged.

[0027] In summary, the present application includes at least one of the following beneficial technical effects: A high temperature chamber and a low temperature chamber are arranged on one side of the heating chamber near the bottom of the temperature control box. The high temperature chamber heats the water therein to the temperature required for DNA denaturation, and the low temperature chamber heats the water therein to the temperature required for DNA annealing. After the sample tube is loaded onto the sample support plate, the water in the high temperature chamber is pumped into the heating chamber by a first water pump, so as to heat the DNA in the sample tube. After heating for a specified time, the first switch mechanism controls the first water outlet to open, so that the water in the heating chamber flows back into the high temperature chamber through the first water outlet. The first switch mechanism controls the first water outlet to close, and the air cooling mechanism is used to External air is drawn into the heating chamber to cool the sample tube. After the sample tube is cooled to the annealing temperature, the water in the low-temperature chamber is pumped into the heating chamber by the second water pump, so that the sample tube is kept at the annealing temperature. After a certain period of time, the second switch mechanism is used to control the second water outlet to open, so that the water in the heating chamber flows back into the low-temperature chamber through the second water outlet. The above process is repeated according to the replication requirements. The heat loss is accelerated by air cooling, and the water temperature in the heating chamber is changed by coordinating the high-temperature chamber and the low-temperature chamber, which helps to quickly reach and maintain the required temperature conditions during the PCR amplification process, thereby improving the amplification efficiency and accuracy. By respectively providing an air inlet and an air outlet on two opposite side walls of the temperature control box, a negative pressure fan is connected to the air outlet, and the negative pressure fan is used to generate negative pressure to extract the hot air in the heating chamber to achieve rapid heat dissipation. The air inlet and the air outlet are both arranged near the top of the heating chamber, so that the hot air can be more efficiently extracted from the high-temperature area and discharged out of the temperature control box through the negative pressure fan. This design helps to reduce the temperature gradient and ensure that the temperature in the heating chamber is more uniform. In addition, the arrangement of the air inlet and the air outlet is likely to cause changes in the water temperature in the heating chamber. A heating tube is arranged in the heating chamber, and the water temperature in the heating chamber is detected by a temperature detection sensor. The temperature detection sensor is electrically connected to the heating tube. The heating tube heats the water in the heating chamber according to the data detected by the temperature detection sensor, so that the water in the heating chamber is maintained at the set temperature, thereby improving the amplification effect of the PCR device. By fixing the electromagnet on the inner side of the cover, a ferromagnetic metal block is correspondingly arranged on the side of the upper cover close to the cover. Before the cover contacts the trigger switch, the electromagnet is used to adsorb the ferromagnetic metal block, so that the upper cover moves in the direction close to the cover, thereby reducing the influence of the temperature detection probe on the closing of the upper cover. When the upper cover is closed on the temperature control box, the upper cover contacts the trigger switch, the trigger switch controls the electromagnet to cut off the power, and the cover moves in the direction away from the upper cover under the elastic force of the spring. During the movement of the cover, the temperature detection probe is inserted into the corresponding sample tube, and the sample tube is closed by the cover at the same time. The movement of the cover is guided by the cooperation of the first guide column and the second guide column, thereby improving the stability of the movement of the cover. In addition, the sample tube is protected by a rubber ring arranged on the sample support plate, thereby reducing the possibility of the sample tube being squeezed and damaged. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings: Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present application; Figure 2 is a schematic diagram of the structure of the first switch mechanism part and the second switch mechanism part in the embodiment of the present application; Figure 3 It is a structural schematic diagram of the air cooling mechanism part and the sample support plate part in the embodiment of the present application; Figure 4 It is a structural schematic diagram of the heat preservation mechanism part in the embodiment of the present application.

[0029] Figure numerals: 100, temperature control box; 110, heating chamber; 111, air inlet; 112, air outlet; 120, sample support plate; 121, loading hole; 130, high temperature chamber; 131, first water outlet; 140, low temperature chamber; 141, second water outlet; 150, first switch mechanism; 151, first electric push rod; 152, first switch plate; 153, first water hole; 160, second switch mechanism; 161, second electric push rod; 162, second switch Plate; 163, second water hole; 170, first water pump; 180, second water pump; 190, heating tube; 200, heat preservation mechanism; 210, upper cover; 220, cover plate; 230, spring; 240, temperature detection probe; 250, electromagnet; 260, ferromagnetic metal block; 270, first guide column; 280, second guide column; 300, air cooling mechanism; 310, negative pressure fan; 400, temperature detection sensor; 500, rubber ring; 600, sample tube. DETAILED DESCRIPTION

[0030] In order to more clearly explain the overall concept of this application, the following Figure 1-Figure 4 This application is described in further detail.

[0031] In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present application is not limited by the specific embodiments disclosed below. It should be noted that the embodiments of the present application and the features in each embodiment may be combined with each other without conflict.

[0032] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0033] The present application embodiment discloses a PCR amplification device. Figure 1 , Figure 2 and Figure 3A PCR amplification device includes a temperature control box 100, a heat preservation mechanism 200, an air cooling mechanism 300 and a controller. A heating chamber 110 is provided at the upper end of the temperature control box 100, and a sample support plate 120 is provided at the top of the heating chamber 110. The sample support plate 120 is connected to the temperature control box 100 and is used to fix the sample tube 600. A high-temperature chamber 130 and a low-temperature chamber 140 are provided on one side of the heating chamber 110 near the bottom of the temperature control box 100. The high-temperature chamber 130 is used to heat the water therein to the temperature required for DNA denaturation, and the low-temperature chamber 140 is used to heat the water therein to the temperature required for DNA annealing. The high-temperature chamber 130 is connected to the heating chamber 110 through a first water outlet 131 provided at the bottom of the heating chamber 110, and the low-temperature chamber 140 is connected to the heating chamber 110 through a second water outlet 141 provided at the bottom of the heating chamber 110. The temperature control box 100 is provided with a first switch mechanism 150 for controlling the opening and closing of the first water outlet 131, and the temperature control box 100 is provided with a second switch mechanism 160 for controlling the opening and closing of the second water outlet 141. The high-temperature chamber 130 is connected to the heating chamber 110 through the first water pump 170, and the low-temperature chamber 140 is connected to the heating chamber 110 through the second water pump 180. The first switch mechanism 150, the second switch mechanism 160, the first water pump 170 and the second water pump 180 are all electrically connected to the controller.

[0034] The air cooling mechanism 300 includes a negative pressure fan 310, which is fixedly connected to the outer wall of the temperature control box 100 and is electrically connected to the controller. An air inlet 111 and an air outlet 112 are respectively provided on the two opposite side walls of the temperature control box 100, one end of the air inlet 111 is connected to the heating chamber 110, and the other end of the air inlet 111 is provided on the outer wall of the temperature control box 100, the air inlet 111 is arranged near the top of the heating chamber 110, one end of the air outlet 112 is connected to the heating chamber 110, and the other end of the air outlet 112 is connected to the air inlet 111 of the negative pressure fan 310, and the air outlet 112 is arranged near the top of the heating chamber 110. A heating pipe 190 is arranged in the heating chamber 110, and a temperature detection sensor 400 is arranged in the heating chamber 110, and both the temperature detection sensor 400 and the heating pipe 190 are electrically connected to the controller. The heat preservation mechanism 200 is disposed at the upper end of the temperature control box 100 , and the heat preservation mechanism 200 is used to separate the space above the temperature control box 100 from the external space.

[0035] By arranging a high temperature chamber 130 and a low temperature chamber 140 on one side of the heating chamber 110 near the bottom of the temperature control box 100, the high temperature chamber 130 heats the water therein to the temperature required for DNA denaturation, and the low temperature chamber 140 heats the water therein to the temperature required for DNA annealing. After the sample tube 600 is loaded onto the sample support plate 120, the water in the high temperature chamber 130 is pumped into the heating chamber 110 by the first water pump 170, so as to heat the DNA in the sample tube 600. After heating for a predetermined time, the first switch mechanism 150 controls the first water outlet 131 to open, so that the water in the heating chamber 110 flows back into the high temperature chamber 130 through the first water outlet 131, and the first switch mechanism 150 controls the first water outlet 131 to close. The negative pressure fan 310 is connected to the air outlet 112, and the negative pressure fan 310 is used to generate negative pressure to extract the hot air in the heating chamber 110. After the external air enters the heating chamber 110, the sample tube 600 is cooled. After the sample tube 600 is cooled to the annealing temperature, the second water pump 180 is used to pump the water in the low temperature chamber 140 into the heating chamber 110, so that the sample tube 600 maintains the annealing temperature. After a certain period of time, the second switch mechanism 160 is used to control the second water outlet 141 to open, so that the water in the heating chamber 110 flows back to the low temperature chamber 140 through the second water outlet 141, and the above process is repeated according to the replication requirements. The heat loss is accelerated by air cooling, and the water temperature in the heating chamber 110 is changed in coordination with the high temperature chamber 130 and the low temperature chamber 140, which helps to quickly reach and maintain the required temperature conditions during the PCR amplification process, thereby improving the amplification efficiency and accuracy.

[0036] By arranging the air inlet 111 and the air outlet 112 near the top of the heating chamber 110, hot air can be more efficiently drawn out of the high temperature area and discharged from the temperature control box 100 through the negative pressure fan 310. This design helps to reduce the temperature gradient and ensure that the temperature in the heating chamber 110 is more uniform. In addition, the arrangement of the air inlet 111 and the air outlet 112 easily leads to changes in the water temperature in the heating chamber 110. By arranging a heating tube 190 in the heating chamber 110, the water temperature in the heating chamber 110 is detected by a temperature detection sensor 400, and the temperature detection sensor 400 is electrically connected to the heating tube 190. The heating tube 190 heats the water in the heating chamber 110 according to the data detected by the temperature detection sensor 400, so that the water in the heating chamber 110 is maintained at the set temperature, thereby improving the amplification effect of the PCR device.

[0037] Reference Figure 2 and Figure 3, a plurality of first water outlets 131 are provided. The first switch mechanism 150 includes a first electric push rod 151 and a first switch plate 152. A plurality of first water holes 153 are provided on the first switch plate 152. The plurality of first water holes 153 are arranged in a one-to-one correspondence with the plurality of first water outlets 131. The first electric push rod 151 is fixedly connected to the outer wall of the temperature control box 100. The piston rod of the first electric push rod 151 passes through the outer wall of the temperature control box 100 and is fixedly connected to the first switch plate 152. The first electric push rod 151 drives the first switch plate 152 to slide in the horizontal direction. The first water holes 153 on the first switch plate 152 can be connected to the first water outlet 131 under the drive of the first electric push rod 151.

[0038] Reference Figure 2 and Figure 3 , the second water outlet 141 is provided with a plurality of them. The second switch mechanism 160 includes a second electric push rod 161 and a second switch plate 162, and the second switch plate 162 is provided with a plurality of second water holes 163, and the plurality of second water holes 163 are arranged in a one-to-one correspondence with the plurality of second water outlets 141. The second electric push rod 161 is fixedly connected to the outer wall of the temperature control box 100, and the piston rod of the second electric push rod 161 passes through the outer wall of the temperature control box 100 and is fixedly connected to the second switch plate 162, and the second electric push rod 161 drives the second switch plate 162 to slide in the horizontal direction, and the second water holes 163 on the second switch plate 162 can be connected to the second water outlet 141 under the drive of the second electric push rod 161.

[0039] Reference Figure 1 , Figure 2 and Figure 4 The heat preservation mechanism 200 includes an upper cover 210 and a cover plate 220. The upper cover 210 is hinged to the temperature control box 100, and the upper cover 210 is used to separate the space above the temperature control box 100 from the external space. The cover plate 220 is connected to the inner side of the temperature control box 100, and the upper cover 210 is used to seal the tube opening of the sample tube 600.

[0040] Reference Figure 1 , Figure 2 and Figure 4 The cover plate 220 is elastically connected to the upper cover 210 through a spring 230. A plurality of temperature detection probes 240 are arranged on the cover plate 220. The plurality of temperature detection probes 240 are arranged one by one corresponding to the plurality of sample tubes 600. The temperature detection probes 240 are used to detect the temperature in the corresponding sample tube 600. The controller is fixedly connected to the outer side of the cover plate 220, and the plurality of temperature detection probes 240 are electrically connected to the controller. By arranging the cover plate 220 on the inner side of the upper cover 210, the cover plate 220 is used to seal the tube mouth of the sample tube 600, thereby reducing the heat loss of the sample tube 600 at the tube mouth, thereby improving the heating efficiency of the sample tube 600.

[0041] Reference Figure 1 , Figure 2 and Figure 4 The cover plate 220 is elastically connected to the upper cover 210 through a spring 230, an electromagnet 250 is fixedly connected to the inner side of the cover plate 220, and a ferromagnetic metal block 260 is correspondingly provided on the side of the upper cover 210 close to the cover plate 220. A first guide column 270 is fixedly connected to the inner side of the cover plate 220, and a second guide column 280 is fixedly connected to the side of the upper cover 210 close to the cover plate 220. A connection hole is provided at one end of the first guide column 270 away from the cover plate 220, and one end of the second guide column 280 away from the cover plate 220 is plugged into the connection hole. A trigger switch is provided at the upper end of the temperature control box 100, and the trigger switch is used to detect whether the upper cover 210 is covered on the top of the temperature control box 100, and the trigger switch is electrically connected to the electromagnet 250.

[0042] By fixing the electromagnet 250 on the inner side of the cover plate 220 , a ferromagnetic metal block 260 is correspondingly provided on one side of the upper cover 210 close to the cover plate 220 . In this embodiment, the ferromagnetic metal block 260 is an iron block. Before the cover 220 contacts the trigger switch, the electromagnet 250 is used to adsorb the ferromagnetic metal block 260, so that the upper cover 210 moves toward the direction close to the cover 220, reducing the influence of the temperature detection probe 240 on the closing of the upper cover 210. When the upper cover 210 is closed on the temperature control box 100, the upper cover 210 contacts the trigger switch, and the trigger switch controls the electromagnet 250 to cut off the power. The cover 220 moves away from the upper cover 210 under the elastic force of the spring 230. During the movement of the cover 220, the temperature detection probe 240 is inserted into the corresponding sample tube 600. At the same time, the cover 220 is used to close the sample tube 600. The movement of the cover 220 is guided by the cooperation of the first guide column 270 and the second guide column 280, thereby improving the stability of the movement of the cover 220.

[0043] Reference Figure 1 , Figure 2 and Figure 4 The sample support plate 120 is provided with a plurality of loading holes 121 for the sample tube 600 to pass through, and a plurality of rubber rings 500 are fixedly connected to the side of the sample support plate 120 away from the heating chamber 110. The plurality of rubber rings 500 are arranged one by one corresponding to the plurality of loading holes 121, and the rubber rings 500 are coaxially arranged with the corresponding loading holes 121, and the inner diameter of the rubber ring 500 is smaller than the inner diameter of the loading hole 121, and the sample tube 600 is interference-connected with the rubber ring 500.

[0044] By fixing a plurality of rubber rings 500 on the side of the sample support plate 120 away from the heating chamber 110, and the plurality of rubber rings 500 are arranged one by one corresponding to the plurality of loading holes 121, when the sample tube 600 is loaded on the sample support plate 120, the rubber ring 500 first passes through the rubber ring 500 and then passes through the loading hole 121, and the friction between the inner wall of the rubber ring 500 and the outer wall of the sample tube 600 is used to limit the sample tube 600, reducing the possibility of the sample tube 600 floating up during heating, thereby improving the heating stability of the sample tube 600. In addition, the rubber ring 500 arranged on the sample support plate 120 protects the sample tube 600, reducing the possibility of the sample tube 600 being squeezed and damaged.

[0045] Anything not described in this application can be achieved by adopting or drawing on existing technologies.

[0046] The various embodiments in this specification are described in a progressive manner, and the same or similar parts between the various embodiments can be referenced to each other, and each embodiment focuses on the differences from other embodiments.

[0047] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A PCR amplification device, characterized in that: The invention comprises a temperature control box (100) and a heat preservation mechanism (200); a heating chamber (110) is provided at the upper end of the temperature control box (100); a sample support plate (120) is provided at the top of the heating chamber (110); the sample support plate (120) is connected to the temperature control box (100); the sample support plate (120) is used to fix the sample tube (600); a high-temperature chamber (130) and a low-temperature chamber (140) are provided on one side of the heating chamber (110) close to the bottom of the temperature control box (100); the high-temperature chamber (130) is used to heat water therein to a temperature required for DNA denaturation; the low-temperature chamber (140) is used to heat water therein to a temperature required for DNA annealing; the high-temperature chamber (130) is connected to the heating chamber (110) via a first water outlet (131) provided at the bottom of the heating chamber (110); and the low-temperature chamber (140) is connected to the heating chamber (110) via a first water outlet (131) provided at the bottom of the heating chamber (110); ) is connected to the heating chamber (110); a first switch mechanism (150) for controlling the opening and closing of the first water outlet (131) is provided on the temperature control box (100); a second switch mechanism (160) for controlling the opening and closing of the second water outlet (141) is provided on the temperature control box (100); the high-temperature chamber (130) is connected to the heating chamber (110) via a first water pump (170); the low-temperature chamber (140) is connected to the heating chamber (110) via a second water pump (180); the heating chamber (110) is connected to an air cooling mechanism (300); the air cooling mechanism (300) is used to draw external air into the heating chamber (110) to cool the sample tube (600); the heat preservation mechanism (200) is provided at the upper end of the temperature control box (100); the heat preservation mechanism (200) is used to separate the space above the temperature control box (100) from the external space.

2. A PCR amplification device according to claim 1, characterized in that: The air cooling mechanism (300) comprises a negative pressure fan (310), the negative pressure fan (310) being fixedly connected to the outer wall of the temperature control box (100), and an air inlet (111) and an air outlet (112) are respectively provided on two opposite side walls of the temperature control box (100), one end of the air inlet (111) is connected to the heating chamber (110), and the other end of the air inlet (111) is provided on the outer wall of the temperature control box (100), and the air inlet (111) is provided near the top of the heating chamber (110). The heating chamber (110) is provided with a heating pipe (190) disposed inside the heating chamber (110), and a temperature detection sensor (400) is disposed inside the heating chamber (110), wherein the temperature detection sensor (400) is electrically connected to the heating pipe (190).

3. A PCR amplification device according to claim 1, characterized in that: A plurality of first water outlets (131) are provided, the first switch mechanism (150) comprises a first electric push rod (151) and a first switch plate (152), a plurality of first water holes (153) are provided on the first switch plate (152), the plurality of first water holes (153) are arranged in a one-to-one correspondence with the plurality of first water outlets (131), the first electric push rod (151) is fixedly connected to the outer wall of the temperature control box (100), a piston rod of the first electric push rod (151) passes through the outer wall of the temperature control box (100) and is fixedly connected to the first switch plate (152), the first electric push rod (151) drives the first switch plate (152) to slide in a horizontal direction, and the first water holes (153) on the first switch plate (152) can be connected to the first water outlet (131) under the drive of the first electric push rod (151).

4. A PCR amplification device according to claim 3, characterized in that: The second water outlets (141) are provided with a plurality of them. The second switch mechanism (160) comprises a second electric push rod (161) and a second switch plate (162). The second switch plate (162) is provided with a plurality of second water holes (163). The plurality of second water holes (163) are arranged in a one-to-one correspondence with the plurality of second water outlets (141). The second electric push rod (161) is fixedly connected to the outer wall of the temperature control box (100). The piston rod of the second electric push rod (161) passes through the outer wall of the temperature control box (100) and is fixedly connected to the second switch plate (162). The second electric push rod (161) drives the second switch plate (162) to slide in a horizontal direction. The second water holes (163) on the second switch plate (162) can be connected to the second water outlets (141) under the drive of the second electric push rod (161).

5. A PCR amplification device according to claim 1, characterized in that: The sample support plate (120) is provided with a plurality of loading holes (121) for allowing the sample tubes (600) to pass through; a plurality of rubber rings (500) are fixedly connected to a side of the sample support plate (120) away from the heating chamber (110); the plurality of rubber rings (500) are arranged in a one-to-one correspondence with the plurality of loading holes (121); the rubber rings (500) are coaxially arranged with the corresponding loading holes (121); the inner diameter of the rubber rings (500) is smaller than the inner diameter of the loading holes (121); and the sample tubes (600) are interference-connected with the rubber rings (500).

6. A PCR amplification device according to claim 1, characterized in that: The heat preservation mechanism (200) comprises an upper cover (210) and a cover plate (220); the upper cover (210) is hinged to the temperature control box (100); the upper cover (210) is used to separate the space above the temperature control box (100) from the external space; the cover plate (220) is connected to the inner side of the temperature control box (100); and the upper cover (210) is used to seal the tube opening of the sample tube (600).

7. A PCR amplification device according to claim 6, characterized in that: The cover plate (220) is elastically connected to the upper cover (210) via a spring (230); a plurality of temperature detection probes (240) are arranged on the cover plate (220); the plurality of temperature detection probes (240) are arranged in a one-to-one correspondence with the plurality of sample tubes (600); the temperature detection probes (240) are used to detect the temperature in the corresponding sample tube (600); a controller is arranged on the outer side of the cover plate (220); the plurality of temperature detection probes (240) are all electrically connected to the controller; and the temperature detection sensor (400), the first water pump (170), the second water pump (180), the first electric push rod (151), the second electric push rod (161) and the heating tube (190) are all electrically connected to the controller.

8. A PCR amplification device according to claim 7, characterized in that: An electromagnet (250) is fixedly connected to the inner side of the cover plate (220); a ferromagnetic metal block (260) is correspondingly arranged on the side of the upper cover (210) close to the cover plate (220); a first guide column (270) is fixedly connected to the inner side of the cover plate (220); a second guide column (280) is fixedly connected to the side of the upper cover (210) close to the cover plate (220); a connection hole is provided at one end of the first guide column (270) away from the cover plate (220); one end of the second guide column (280) away from the cover plate (220) is plugged into the connection hole; a trigger switch is arranged at the upper end of the temperature control box (100); the trigger switch is used to detect whether the upper cover (210) is covered on the upper side of the temperature control box (100); and the trigger switch is electrically connected to the electromagnet (250).

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