Red light OLED (Organic Light Emitting Diode) light-emitting device for enhancing cell activity

Abstract

The invention belongs to the technical field of red-light OLEDs, and particularly relates to a red-light OLED light-emitting device for enhancing cell activity. The red light OLED light-emitting device for enhancing the cell activity comprises an upper base layer, a cathode layer, an EIL electron injection layer, an upper ETL electron transport layer, an upper red light emitting layer, an upper EBL electron barrier layer, an upper HTL hole transport layer, an upper CGL charge generation layer, a middle ETL electron transport layer, a middle red light emitting layer and a middle EBL electron barrier layer which are fully laminated in sequence from top to bottom, the device comprises a middle HTL hole transport layer, a middle CGL charge generation layer, a lower ETL electron transport layer, a lower red light emitting layer, a lower EBL electron barrier layer, a lower HTL hole transport layer, a lower HIL hole transport layer, an anode layer and a lower base layer. The light-emitting device for enhancing the cell activity through the red OLED has a positive effect on cells which emit light through the OLED, and has an effect of irradiating deep cells through a structure with three times of brightness.

Description

Technical Field

[0001] The invention belongs to the technical field of red light OLED, and in particular relates to a red light OLED light emitting device for enhancing cell activity. Background Art

[0002] The original name of OLED is Organic Light Emitting Diode, which means "organic light-emitting display technology" in Chinese. Its principle is to sandwich an organic light-emitting layer between two electrodes. When positive and negative electrons meet in this organic material, they will emit light. Its component structure is simpler than the currently popular TFT LCD, and its production cost is only about 30% to 40% of TFT LCD. In addition to the low production cost, OLED has many advantages, such as its own light-emitting characteristics. Currently, LCDs all need a backlight module (add a lamp behind the liquid crystal), but OLED will emit light by itself after power is turned on, which can save the weight, volume and power consumption of the lamp (the power consumption of the lamp accounts for almost half of the entire LCD screen). Not only does it make the product thickness only about two centimeters, the operating voltage is lower to 2 to 10 volts, and the response time (less than 10ms) and color of OLED are better than TFT LCD, and it has the characteristic of being flexible, which makes its application range extremely wide.

[0003] An existing Chinese patent with publication number CN105492888A discloses a cell culture and tracking system that uses an array of organic light emitting diodes (OLEDs) to illuminate cells and / or other particles in a cell chamber. Compared to conventional light sources, the OLED array consumes very little energy and emits a small amount of waste heat, so it can be arranged near or on the cell chamber. For example, it can be printed on one side of the cell chamber itself. In addition, the OLED array can be patterned into pixels or sub-pixels (individual OLEDs), each pixel or sub-pixel being as small as or smaller than an individual cell or particle. Because the pixels are so small, OLED illumination can be used to obtain images with a spatial resolution equal to or better than that of cells or particles.

[0004] The above-mentioned prior art solutions have the following defects: although OLED arrays can be used to track, monitor, identify and manipulate individual cells in cell culture, there is no mention of the effect of OLED light-emitting devices on cells, and OLED can only illuminate cells on the surface of the culture dish and cannot interfere with deep cells. Summary of the invention

[0005] The purpose of the present invention is to provide a red light OLED light-emitting device that enhances cell activity, so as to solve the technical problem that OLED light-emitting devices have no effect on cells and can only illuminate cells on the inner surface of the culture dish but cannot interfere with deep cells, so as to achieve the purpose of having a positive effect on cells emitted by OLED and illuminating deep cells through a structure with three times the brightness.

[0006] In order to solve the above technical problems, the present invention provides a red light OLED light emitting device for enhancing cell activity, comprising: Upper base layer, cathode layer, EIL electron injection layer, upper ETL electron transport layer, upper red light emitting layer, upper EBL electron blocking layer, upper HTL hole transport layer, upper CGL charge generation layer, middle ETL electron transport layer, middle red light emitting layer, middle EBL electron blocking layer, middle HTL hole transport layer, middle CGL charge generation layer, lower ETL electron transport layer, lower red light emitting layer, lower EBL electron blocking layer, lower HTL hole transport layer, lower HIL hole transport layer, anode layer and lower base layer.

[0007] Further, the thickness of the cathode layer is 2245-2255 nanometers; The thickness of the anode layer is 130-140 nanometers, the transmittance is greater than 85%, and the square cathode is 8-12 ohms.

[0008] Furthermore, the thickness of the EIL electron injection layer is 10-20 nanometers.

[0009] Furthermore, the thickness of the upper ETL electron transport layer, the middle ETL electron transport layer and the lower ETL electron transport layer are all 90-110 nanometers.

[0010] Furthermore, the thickness of the upper EBL electron blocking layer, the middle EBL electron blocking layer and the lower EBL electron blocking layer is 90-110 nanometers.

[0011] Furthermore, the thickness of the upper red light emitting layer, the middle red light emitting layer and the lower red light emitting layer is 295-305 nanometers.

[0012] Furthermore, the thickness of the upper EBL electron blocking layer, the middle EBL electron blocking layer and the lower EBL electron blocking layer is 90-110 nanometers.

[0013] Furthermore, the thickness of the upper HTL hole transport layer, the middle HTL hole transport layer and the lower HTL hole transport layer is 145-155 nanometers.

[0014] Furthermore, the thickness of the upper CGL charge generation layer and the middle CGL charge generation layer is 195-205 nanometers.

[0015] Furthermore, the upper base layer and the lower base layer are made of light-transmitting glass with a thickness of 0.1-2 nanometers, and are made of one or more of sodium-lime white material, medium aluminum or high aluminum.

[0016] The beneficial effects of the present invention are: 1. Through three layers of red light emitting layers, it forms three times the brightness, irradiates deep, and stimulates deeper cells.

[0017] 2. OLED red light is closer to the solar spectrum. Photons of different wavelengths reach each layer of subcutaneous cells directly, allowing mitochondria to absorb better. The energy conversion rate is high, which can better activate cell regeneration and promote blood circulation.

[0018] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 It is a schematic structural diagram of a red light OLED light emitting device for enhancing cell activity according to the present invention; Figure 2 It is a schematic diagram of the red light wavelength and color tolerance of the present invention.

[0021] In the figure: 1. Upper grassroots; 2. Cathode layer; 3. EIL electron injection layer; 4. Upper ETL electron transport layer; 5. Upper red light emitting layer; 6. Upper EBL electron blocking layer; 7. Upper HTL hole transport layer; 8. Upper CGL charge generation layer; 9. Middle ETL electron transport layer; 10. Middle red light emitting layer; 11. Middle layer EBL electron blocking layer; 12. Middle HTL hole transport layer; 13. Middle CGL charge generation layer; 14. Lower ETL electron transport layer; 15. Lower red light emitting layer; 16. Lower layer EBL electron blocking layer; 17. Lower HTL hole transport layer; 18. Lower HIL hole transport layer; 19. Anode layer; 20. Lower level grass-roots. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example: Figure 1 to Figure 2 As shown, a red light OLED light-emitting device for enhancing cell activity includes: Upper base layer 1, cathode layer 2, EIL electron injection layer 3, upper ETL electron transport layer 4, upper red light emitting layer 5, upper EBL electron blocking layer 6, upper HTL hole transport layer 7, upper CGL charge generation layer 8, middle ETL electron transport layer 9, middle red light emitting layer 10, middle EBL electron blocking layer 11, middle HTL hole transport layer 12, middle CGL charge generation layer 13, lower ETL electron transport layer 14, lower red light emitting layer 15, lower EBL electron blocking layer 16, lower HTL hole transport layer 17, lower HIL hole transport layer 18, anode layer 19 and lower base layer 20.

[0024] Figure 1 In the figure, the arrows indicate the irradiation directions of the red light emitted by the upper red light emitting layer 5 , the middle red light emitting layer 10 and the lower red light emitting layer 15 .

[0025] The functions of the upper EBL electron blocking layer 6 , the middle EBL electron blocking layer 11 and the lower EBL electron blocking layer 16 are to prevent electron leakage.

[0026] In this embodiment, the upper base layer 1 and the lower base layer 20 are made of light-transmitting glass with a thickness of 0.1-2 nanometers, and are made of one or more of soda-lime white material, medium aluminum or high aluminum.

[0027] The thickness of the cathode layer 2 is 2245-2255 nanometers, the thickness of the anode layer 19 is 130-140 nanometers, the light transmittance is greater than 85%, and the square cathode is 8-12 ohms.

[0028] The thickness of the EIL electron injection layer 3 is 10-20 nanometers. The thickness of the upper ETL electron transport layer 4, the middle ETL electron transport layer 9 and the lower ETL electron transport layer 14 are all 90-110 nanometers.

[0029] The thickness of the upper EBL electron blocking layer 6, the middle EBL electron blocking layer 11 and the lower EBL electron blocking layer 16 is 90-110 nanometers.

[0030] like Figure 1 As shown, the thickness of the upper red light emitting layer 5, the middle red light emitting layer 10 and the lower red light emitting layer 15 is 295-305 nanometers.

[0031] like Figure 1 and Figure 2 As shown, the dominant wavelength of the upper red light emitting layer 5, the middle red light emitting layer 10 and the lower red light emitting layer 15 is 640 nanometers, the color purity is Pur=100.08, and the centroid wavelength is 650nm, wherein, the chromaticity coordinates: x=0.7013y=0.2985, the chromaticity coordinates: u'=0.5416v'=0.5187 (duv=-9.41e-02), the correlated color temperature: Tc=1001K, the dominant wavelength: λd=625.5nm, the color purity: Pur=100.08, the centroid wavelength: 649.0n, the color ratio: R=96.4% G=3.6%, B=0.0% peak wavelength: λp=640.0nm, and the half width: ΔÀp=43.7nm.

[0032] The thickness of the upper EBL electron blocking layer 6, the middle EBL electron blocking layer 11 and the lower EBL electron blocking layer 16 is 90-110 nanometers.

[0033] The thickness of the upper HTL hole transport layer 7, the middle HTL hole transport layer 12 and the lower HTL hole transport layer 17 is 145-155 nanometers. The thickness of the upper CGL charge generation layer 8 and the middle CGL charge generation layer 13 is 195-205 nanometers.

[0034] The red light OLED light-emitting device for enhancing cell activity of the present application has a low lighting temperature and is in a touchable state. At the same time, the OLED light source is not dazzling, does not need to be hidden, and has a simple structure. The OLED light source has a variety of forms and can be flexible and transparent. The present application is a surface light source, the light is soft and not dazzling, and the OLED light source has good color rendering, restoring the most real color of the object.

[0035] This application is a full-band red light, with multiple bands reaching different areas of cell divisions with high conversion rates, which can better activate cell regeneration and promote blood circulation, thereby achieving the purpose of stimulating cells. At the same time, Photoelectric detection of the OLED of this application:

[0036] As shown above, the actual luminous area is reduced by ~10mm compared to the external size. The spectral range is at half-peak height, and the radiation energy is measured with an integrating sphere at maximum power, so that the red light band can reach cells at different depths.

[0037] The following is the experimental data of this application: 1 Materials 1.1 Cells HaCaT (human immortalized skin keratinocyte cell line) was purchased from the Cell Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

[0038] HFF (human dermal fibroblasts) were purchased from the Cell Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

[0039] 1.2 Cell culture related reagents DMEM medium (Gbico, USA, 11995073): fetal bovine serum (FBS) (Gbico, USA, 10099158); penicillin-streptomycin-amphotericin B supplement (Sevier, China, G4015); phosphate buffered saline (PBS) (Sevier, China, G4207): CCK8 (APExBIO, USA, K1018): SYBR (Aikerui, China, AG11740): RNAisoPlus (Takara, China, 9108Q); reverse transcription reagent (Novozyme, China, R423-01).

[0040] 1.3 Experimental instruments Microplate reader (Tecan, Spark, Switzerland), real-time fluorescence quantitative PCR instrument (Roche, LightCycler480, Switzerland); Experimental Method 2 2.1 Cell culture Resuscitate HaCaT cells in DMEM medium containing 10% fetal bovine serum and 1% triple antibody at 37°C within 1 minute: Resuscitate HFF cells in DMEM medium containing 15% fetal bovine serum and 1% triple antibody at 37°C within 1 minute. Culture in an incubator at 37°C and 5% CO2, subculture once every 48h-72h, with a subculture ratio of 1.3.

[0041] 2.2 Detection of the effects of different red light doses on cell proliferation (1) Collect cells in the exponential growth phase and count them according to 1x10 4 HaCaT and HFF cells were plated in 96-well plates with 200 μL complete DMEM medium per well. Each group had 3 replicate wells: (2) After 24 h of culture, the culture medium was replaced with phenol red-free culture medium, and the cells were placed 5 cm below the red light source and the intensity gradient (0 J / cm 2 , 15J / cm 2 , 30J / cm 2 , 45J / cm 2 , 60J / cm 2 , 75J / cm 2 , 90J / cm 2 , 105J / cm 2 , 120J / cm 2 ) for red light irradiation; (3) After red light irradiation, complete DMEM medium was added again, and after culturing at 37°C for 24 h, 10 μL CCK8 reagent was added to each well to detect cell proliferation ability.

[0042] In summary: through three layers of red light emitting layers, triple brightness is achieved. In addition, OLED red light is closer to the solar spectrum, and photons of different wavelengths directly reach each layer of subcutaneous cells for better absorption by mitochondria. The energy conversion rate is high, which can better activate cell regeneration and promote blood circulation.

[0043] 2.3 Detection of the effects of red light irradiation on skin barrier and inflammation-related genes (1) Collect cells in the exponential growth phase and calculate 2x10 5 HaCaT cells were inoculated into 3.5 cm culture dishes at 1 cell / dish (2 ml culture medium) and irradiated with red light according to the dose selected in 2.2.

[0044] (2) The cells were cultured at 37°C for 24 h, and cellular RNA was extracted using the TRIzol method.

[0045] (4) Use a kit to reverse transcribe RNA to obtain cDNA.

[0046] (5) qPCR was used to detect the expression of keratin-related gene KRT and skin inflammation-related genes IL1, IL6, and TNF-a in HaCaT cells.

[0047] 2.4 Detection of the effect of red light irradiation on genes related to skin aging (1) Collect cells in the exponential growth phase and calculate 2x10 5 HFF cells were inoculated into 3.5 cm culture dishes at a concentration of 1 cell / dish (2 ml culture medium) and irradiated with red light according to the dose selected in 2.2.

[0048] (2) The cells were cultured at 37°C for 24 h, and cellular RNA was extracted using the TRIzol method.

[0049] (4) Use a kit to reverse transcribe RNA to obtain cDNA.

[0050] (5) qPCR was used to detect the expression of skin aging-related genes Col-1a1 and MMP in HFF cells.

[0051] 3 Experimental results 3.1 Effects of different red light doses on cell proliferation 3.1. With the increase of red light irradiation dose, the activity of HaCaT cells did not decrease significantly, indicating that the red light OLED light-emitting device of the present application can enhance the activity of cells at 120 J / cm 2 The irradiation dose range is safe for HaCaT cells. As the red light irradiation dose increases, the activity of HFF cells does not decrease significantly, indicating that the red light OLED light-emitting device of the present application for enhancing cell activity is safe for HaCaT cells at 120 J / cm 2 The irradiation dose range is safe for HFF cells. 2 Red light irradiation within the dose range significantly promoted the activity of HFF cells. Therefore, the subsequent experiments used 60J / cm 2 (medium dose) and 120J / cm 2 HaCaT cells and HFF cells were treated with two irradiation doses (higher dose).

[0052] 3.2 Effects of red light irradiation on keratin-related genes Keratin is the main structural protein in keratinocytes. It provides structural support as a cytoskeleton, protects cells from mechanical stress, and provides a platform for cell signal transduction. The basal cells of the epidermis mainly express proliferation-specific keratins KRT14-KRT5, and the cells in the upper basal layer begin to mainly express differentiation-specific keratins KRT10-KRT1. KRT10-KRT1 is an early marker of epidermal differentiation. Red light irradiation 60J / cm 2 Afterwards, the expression of genes related to skin keratin was detected. 2 After treatment, the expression level of KRT5 gene increased by 23.71% compared with that of the control group, and the difference was not statistically significant (P>0.05). The expression level of KRT10 gene increased significantly by 218.92% compared with that of the control group (P<0.01).

[0053] 3.3 Effects of red light irradiation on genes related to skin inflammation When exogenous irritants penetrate the epidermis, they first contact keratinocytes, promoting keratinocytes to produce and release various soluble molecules to cause inflammatory responses, such as cytokines (such as IL-1, IL-6 and TNF-α), chemokines (such as IL-8) and antimicrobial peptides (such as defensins). Red light irradiation 120J / cm 2 Afterwards, the expression of genes related to skin inflammatory factors was detected. Red light irradiation 120J / cm 2 After treatment, the expression of I-1B gene decreased by 60.84% ​​(P<0.05) compared with the control group; the expression of TNF-a decreased by 58.68% compared with the control group, and the difference was not statistically significant.

[0054] 3.4 Effects of red light irradiation on genes related to skin aging Matrix metalloproteinases (MMPs) play a key role in the degradation of collagen. Their main biological characteristics are that the function of fibroblasts in the dermis is damaged, the ability to synthesize and secrete collagen is reduced, and they specifically degrade extracellular matrix components, destroying the normal structure of collagen fibers, and ultimately leading to skin photoaging. Red light irradiation 120J / cm 2 Then the expression of matrix metalloproteinases MMP1 and MMP3 was detected. Red light irradiation 120J / cm 2 After treatment, the expression of MMP1 gene decreased significantly by 26.87% (P<0.05) compared with the control group; the expression of MMP3 gene decreased significantly by 11.31% (P<0.05) compared with the control group.

[0055] Red light irradiation 60J / cm 2 , 120J / cm 2 After that, the gene expression of type I collagen Col-lal was detected. Red light irradiation 60J / cm 2 After treatment, the expression of Col-1a1 gene increased by 33.62% compared with the control group, and the difference was not statistically significant.

[0056] Red light irradiation 120J / cm 2 After treatment, the expression of Col-1a1 gene increased significantly by 82.07% compared with the control group (P<0.05).

[0057] 4 Experimental conclusions Based on the results of in vitro cell experiments under current test conditions, this study found that the red light OLED light-emitting device of this application can enhance cell activity at 120 J / cm 2 The dose range had no significant inhibitory effect on the activity of HaCaT cells and HFF cells, and it had a certain degree of safety. 2The activity of HFF cells can be significantly promoted within the dosage range. The red light OLED light-emitting device for enhancing cell activity of the present application can significantly increase the gene expression of keratin KRT10 in HaCaT cells and significantly down-regulate the gene expression of inflammatory factor IL-1β, indicating that the red light OLED light-emitting device for enhancing cell activity of the present application has the effect of repairing and soothing the skin. The red light OLED light-emitting device for enhancing cell activity of the present application can significantly down-regulate the gene expression of MMP1 / 3 and significantly increase the gene expression of Col-1a1 in HFF cells, indicating that the red light OLED light-emitting device for enhancing cell activity of the present application has the effect of firming and anti-aging on the skin.

[0058] The various devices selected in this application are all universal standard parts or parts known to those skilled in the art, and their structures and principles can be known to those skilled in the art through technical manuals or conventional experimental methods.

[0059] In the description of the embodiments of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0060] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0061] Based on the above ideal embodiments of the present invention, the relevant staff can make various changes and modifications without departing from the technical concept of the present invention through the above description. The technical scope of the present invention is not limited to the contents of the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A red light OLED light emitting device for enhancing cell activity, characterized in that: Including OLED evaporation stacking architecture from top to bottom: An upper base layer (1), a cathode layer (2), an EIL electron injection layer (3), an upper ETL electron transport layer (4), an upper red light emitting layer (5), an upper EBL electron blocking layer (6), an upper HTL hole transport layer (7), an upper CGL charge generation layer (8), a middle ETL electron transport layer (9), a middle red light emitting layer (10), a middle EBL electron blocking layer (11), a middle HTL hole transport layer (12), a middle CGL charge generation layer (13), a lower ETL electron transport layer (14), a lower red light emitting layer (15), a lower EBL electron blocking layer (16), a lower HTL hole transport layer (17), a lower HIL hole transport layer (18), an anode layer (19) and a lower base layer (20).

2. A red light OLED light emitting device for enhancing cell activity as claimed in claim 1, characterized in that: The thickness of the cathode layer (2) is 2245-2255 nanometers; The thickness of the anode layer (19) is 130-140 nanometers, the light transmittance is greater than 85%, and the square cathode is 8-12 ohms.

3. A red light OLED light emitting device for enhancing cell activity as claimed in claim 2, characterized in that: The thickness of the EIL electron injection layer (3) is 10-20 nanometers.

4. A red light OLED light emitting device for enhancing cell activity as claimed in claim 3, characterized in that: The thickness of the upper ETL electron transport layer (4), the middle ETL electron transport layer (9) and the lower ETL electron transport layer (14) are all 90-110 nanometers.

5. A red light OLED light emitting device for enhancing cell activity as claimed in claim 4, characterized in that: The thickness of the upper EBL electron blocking layer (6), the middle EBL electron blocking layer (11) and the lower EBL electron blocking layer (16) is 90-110 nanometers.

6. A red light OLED light emitting device for enhancing cell activity as claimed in claim 5, characterized in that: The thickness of the upper red light emitting layer (5), the middle red light emitting layer (10) and the lower red light emitting layer (15) is 295-305 nanometers; The main wavelength of the upper red light emitting layer (5), the middle red light emitting layer (10) and the lower red light emitting layer (15) is 640 nanometers, the color purity is Pur=100.08, and the centroid wavelength is 650 nm.

7. A red light OLED light emitting device for enhancing cell activity as claimed in claim 6, characterized in that: The thickness of the upper EBL electron blocking layer (6), the middle EBL electron blocking layer (11) and the lower EBL electron blocking layer (16) is 90-110 nanometers.

8. A red light OLED light emitting device for enhancing cell activity as claimed in claim 7, characterized in that: The thickness of the upper HTL hole transport layer (7), the middle HTL hole transport layer (12) and the lower HTL hole transport layer (17) is 145-155 nanometers.

9. A red light OLED light emitting device for enhancing cell activity as claimed in claim 8, characterized in that: The thickness of the upper CGL charge generation layer (8) and the middle CGL charge generation layer (13) is 195-205 nanometers.

10. A red light OLED light emitting device for enhancing cell activity as claimed in claim 9, characterized in that: The upper base layer (1) and the lower base layer (20) are made of light-transmitting glass with a thickness of 0.1-2 nanometers, and are made of one or more of soda-lime white material, medium aluminum or high aluminum.

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