Biosensor strip for measuring sample, electrode structure thereof, and sample measuring method using same

Abstract

Disclosed are a biosensor strip for measuring a sample, an electrode structure thereof, and a sample measuring method using the same. An electrode structure of a biosensor strip according to an embodiment may include a strip recognition electrode for recognizing that the biosensor strip is inserted into a sample measurement device; a sample recognition electrode for recognizing a sample; and a working electrode and a working reference electrode for determining the sample.

Description

Technical Field

[0001] The following embodiments relate to a biosensor strip for measuring a sample, an electrode structure thereof, and a sample measuring method using the same. Background Art

[0002] Quantitative or qualitative analysis of analytes present in biological samples is very important in chemistry and clinical medicine. For example, measuring blood sugar in the blood of diabetic patients or measuring cholesterol, which is a factor of many adult diseases, are representative examples.

[0003] It is known in the art that in electrochemical biosensors using enzyme activity, it is very important to measure the enzyme activity of biological samples (hereinafter referred to as "samples"), such as specific substances in saliva or blood, glucose, uric acid, protein, DNA, sucrose in clinical chemistry tests, GOT (Glutamate-Oxaloacetate Transaminase) or GPT (Glutamate-Pyruvate Transaminase) in liver function tests, etc. quickly and reproducibly. Among them, the biosensor is composed of a recognition site that recognizes the measurement object and a change site that converts it into an electrical signal.

[0004] The recognition site uses biological material, and when the biological material recognizes the object to be measured, a chemical or physical change occurs. The site that converts this change into an electrical signal is the change site, and the recognition site and the change site are collectively referred to as the biosensor electrode.

[0005] The measurement method of the currently commercialized strip biosensor generally utilizes the capillary phenomenon, which is a force stronger than the earth's gravity and is made possible by plasma or chemical surfactant treatment during the manufacturing process, so that the sample contacts and flows into the sample insertion flow path of the biosensor to accumulate the sample in the sample insertion flow path, and then applies the sample measurement signal to the working electrode (Working electrode) and the working reference electrode (Working reference electrode) to measure the response signal to the sample.

[0006] In a conventional biosensor, before measuring a sample, in order to identify whether the sample has contacted the biosensor strip, the sample contact and inflow time points may be identified by applying a sample identification signal to a working electrode and a working reference electrode.

[0007] In this case, since the working electrode and the working reference electrode that should be used in the sample measurement process are also used in the process of identifying whether the sample is in contact, an electric double layer (EDL) may have been formed on the surface of the working electrode and the working reference electrode before the sample is measured. The electric double layer appears when an electric field is applied to the boundary of different substances (electrodes, samples or solutions), and the double layer capacitance (DLC) as the storage capacity of the electric double layer can be included in the response current signal to the sample measured with the application of the sample measurement signal even in a tiny amount. Therefore, the existing technology of forming a double layer on the working electrode and the working reference electrode before measuring the sample has the problem of low sample measurement accuracy.

[0008] Therefore, it is necessary to propose a technology that solves the problems of existing biosensors. Summary of the invention

[0009] Technical issues

[0010] Some embodiments propose an electrode structure of a biosensor strip, which includes a strip identification electrode and a sample identification electrode configured to operate electrically independently from a working electrode and a working reference electrode to prevent the formation of a double electric layer on the surfaces of the working electrode and the working reference electrode before measuring the sample to improve the accuracy of sample measurement.

[0011] In addition, some embodiments provide an electrode structure of a sample sensor strip in which a strip identification electrode and a sample identification electrode both have respective identification reference electrodes to achieve miniaturization of a biosensor strip.

[0012] In addition, one embodiment provides an electrode structure of a biosensor strip, the electrode structure comprising a blank electrode for bringing a sample into contact with a sample insertion channel with a size greater than a preset size.

[0013] However, the problem to be solved by the present invention is not limited to the above-mentioned problem, and various expansions can be made without departing from the technical idea and scope of the present invention.

[0014] Technical Solution

[0015] According to one embodiment, the electrode structure of a biosensor strip for detecting a sample may include: a strip identification electrode for identifying a sample measuring device into which the biosensor strip is inserted; a sample identification electrode for identifying a sample; and a working electrode and a working reference electrode for measuring the sample.

[0016] According to one solution, it may be characterized in that the strip recognition electrode and the sample recognition electrode are configured to work electrically independently from the working electrode and the working reference electrode.

[0017] According to another scheme, it can be characterized in that a working protrusion formed in the working electrode in an area corresponding to the sample insertion flow path of the biosensor strip and at least two working reference protrusions formed in the working reference electrode in an area corresponding to the sample insertion flow path protrude in a direction orthogonal to the direction in which the working electrode and the working reference electrode extend and are alternately arranged.

[0018] According to another embodiment, the identification reference electrode for each of the strip identification electrode and the sample identification electrode is provided to be shared by the strip identification electrode and the sample identification electrode.

[0019] According to another embodiment, the characteristic is that the sample recognition protrusion formed in the area of ​​the sample recognition electrode corresponding to the sample insertion flow path of the biosensor strip and the recognition reference protrusion formed in the area of ​​the recognition reference electrode corresponding to the sample insertion flow path of the biosensor strip protrude in a direction orthogonal to the direction in which the sample recognition electrode and the recognition reference electrode extend and are arranged opposite to each other.

[0020] According to another embodiment, the biosensor strip may further include a blank electrode disposed on one side of the sample insertion flow path of the biosensor strip.

[0021] According to another embodiment, the strip recognition electrode, the sample recognition electrode, the working electrode and the working reference electrode are parallel to each other and spaced apart to extend in one direction.

[0022] According to one embodiment, a biosensor strip for measuring a sample may include: a lower plate having an electrode structure including a strip identification electrode for identifying a biosensor strip inserted into a sample measuring device, a sample identification electrode for identifying a sample, and a working electrode and a working reference electrode for measuring the sample; a middle plate, arranged on the upper portion of the lower plate and including a sample insertion flow path for inserting an enzyme compound that reacts with the sample; and an upper plate, arranged on the upper portion of the middle plate and including an insertion port for inserting the sample into the sample insertion flow path.

[0023] According to one aspect, it may be characterized in that in the lower plate, the strip recognition electrode and the sample recognition electrode are configured to operate electrically independently from the working electrode and the working reference electrode.

[0024] According to another solution, it may be characterized in that in the lower plate, an identification reference electrode for each of the strip identification electrode and the sample identification electrode is provided to be shared by the strip identification electrode and the sample identification electrode.

[0025] According to another embodiment, the lower plate may further include a vacant electrode disposed on one side of the sample insertion flow path of the biosensor strip.

[0026] According to one embodiment, a sample measurement system may include: a biosensor strip having an electrode structure including a strip recognition electrode for identifying that the biosensor strip is inserted into a sample measurement device, a sample recognition electrode for identifying a sample, and a working electrode and a working reference electrode for measuring the sample; and a sample measurement device for identifying whether the biosensor strip is inserted, identifying whether the sample is in contact with the biosensor strip when the biosensor strip is inserted into the sample measurement device, and applying a sample measurement signal to the working electrode and the working reference electrode to measure a response signal to the sample when the sample is in contact with the biosensor strip.

[0027] According to one embodiment, a sample measurement method performed by a sample measurement system including a biosensor strip and a sample measurement device may include: a step of using a strip recognition electrode included in the biosensor strip to identify whether the biosensor strip is inserted into the sample measurement device; when the biosensor strip is inserted into the sample measurement device, a step of using a sample recognition electrode included in the biosensor strip to identify whether a sample is in contact with the biosensor strip; and when the sample is in contact with the biosensor strip, a step of applying a sample measurement signal to a working electrode and a working reference electrode included in the biosensor strip to measure a response signal to the sample.

[0028] According to one embodiment, it may be characterized in that in the step of determining a response signal to the sample, the strip identification electrode and the sample identification electrode are configured to operate electrically independently from the working electrode and the working reference electrode, and the step of identifying whether the biosensor strip is inserted into the sample determination device and the step of identifying whether the sample is in contact with the biosensor strip are performed independently.

[0029] According to another embodiment, the method may further include using a blank electrode disposed on one side of the sample insertion channel of the biosensor strip to make the size of the sample in contact with the sample insertion channel of the biosensor strip larger than a preset size.

[0030] Effects of the Invention

[0031] Some embodiments propose an electrode structure of a biosensor strip, which includes a strip identification electrode and a sample identification electrode that are configured to work electrically independently from a working electrode and a working reference electrode, thereby achieving the technical effect of preventing the formation of a double electric layer on the surfaces of the working electrode and the working reference electrode before measuring the sample, thereby improving the accuracy of sample measurement.

[0032] In addition, some embodiments provide an electrode structure of a sample sensor strip in which the strip identification electrode and the sample identification electrode both have their own identification reference electrodes, thereby achieving the technical effect of miniaturizing the biosensor strip.

[0033] In addition, an embodiment provides an electrode structure of a biosensor strip, which includes an empty electrode of a preset size or larger for contacting a sample with a sample insertion channel, thereby achieving the technical effect of improving the ramet diffusion defect related to the interface material characteristics.

[0034] However, the effects of the present invention are not limited to the above effects, and various extensions can be made within the scope of the technical idea and field of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 is a schematic diagram of a sample measurement system according to an embodiment;

[0036] Figure 2 yes Figure 1 a schematic diagram of the sample collection device shown;

[0037] Figure 3 yes Figure 1 Schematic diagram of the biosensor strip shown;

[0038] Figure 4 yes Figure 3 A schematic diagram of the electrode structure of the lower plate of the biosensor strip shown;

[0039] Figure 5 is a flow chart showing a sample measurement method according to an embodiment;

[0040] Figure 6 It is to illustrate Figure 5 A diagram illustrating an example of the sample measurement method shown. DETAILED DESCRIPTION

[0041] The embodiments of the present invention are described in detail below with reference to the accompanying drawings. However, the present invention is not limited or restricted to the embodiments. In addition, the same reference numerals given in each figure represent the same components.

[0042] In addition, the terminology used in this specification is used to appropriately express the preferred embodiments of the present invention, which may vary according to the intention of the viewer, the user or the practice of the field to which the present invention belongs. Therefore, the definition of these terms should be based on the overall content of this specification. For example, in this specification, the singular also includes the plural, unless specifically mentioned in the sentence. In addition, for the "comprises" and / or "comprising" used in this specification, the components, steps, operations and / or elements mentioned do not exclude the existence or addition of one or more other components, steps, operations and / or elements. In addition, in this specification, the terms first, second, etc. are used to describe various fields, directions, shapes, etc., but these fields, directions, shapes should not be limited by these terms. These terms are only used to distinguish a predetermined area, direction or shape from other areas, directions or shapes. Therefore, the part described as the first part in any embodiment can also be described as the second part in another embodiment.

[0043] In addition, it should be understood that the various embodiments of the present invention are different from each other, but do not need to be mutually exclusive. For example, the specific shapes, structures and characteristics described herein can be implemented by other embodiments on the basis of one embodiment without exceeding the technical concept and scope of the present invention. In addition, it should be understood that the position, configuration or composition of individual components in the scope of each embodiment proposed can be changed without departing from the technical concept and scope of the present invention.

[0044] Hereinafter, a biosensor strip, an electrode structure thereof, a sample measuring system including the same, and a sample measuring method using the same according to embodiments will be described with reference to the accompanying drawings.

[0045] Figure 1 is a schematic diagram of a sample measurement system according to an embodiment, Figure 2 yes Figure 1 A schematic diagram of a sample collection device is shown, Figure 3 yes Figure 1 Schematic diagram of the biosensor strip shown, Figure 4 yes Figure 3 A schematic diagram of the electrode structure of the lower plate of the biosensor strip is shown.

[0046] The sample measuring system 100 according to an embodiment may include a sample measuring device 110 , a biosensor strip 120 , and a sample collection device 160 .

[0047] The sample collection device 160 includes a sample collection portion 161 having a sample collection swab 161 - 1 for collecting a sample, a filter 162 and a compression tube 163 . Figure 2 shown.

[0048] At this time, when the sample is saliva, the sample collecting part 161 can collect saliva through the sample collecting swab 161-1. The saliva collected by the sample collecting part 161 can be provided to the outside world, that is, the biosensor strip 120, through the compression tube 163 after the filter 162 inserted into the compression tube 163 removes interfering substances in the saliva.

[0049] That is, after the sample collection device 160 collects saliva, the sample collection part 161 can be compressed in the compression tube 163 toward the compression tube 163 , and interfering substances contained in the saliva can be removed through the filter 162 .

[0050] The filter 162 may be composed of at least one layer. In particular, in the filter 162, the thickness of the manufactured layer or the number of layers may be adjusted in consideration of the degree of removal of the interfering substance to be removed from the sample, and the degree of filtration may be adjusted in consideration of the size of the interfering substance to be removed from the sample. For example, when it is desired to remove larger molecules and foreign substances present in saliva as interfering substances, the filter 162 may be formed to have a filtration degree capable of removing the larger molecules and foreign substances.

[0051] When the sample collected by the sample collection device 160 is contacted and inserted by the sample collection device 160, the biosensor strip 120 performs the function of sensing the information to be measured (for example, glucose) from the sample from which the interfering substances have been removed, and can provide the sample measurement device 110 with a response signal in response to the strip identification signal received from the sample measurement device 110, a response signal in response to the sample identification signal received from the sample measurement device 110, and a response signal in response to the sample measurement signal received from the sample measurement device 110 when inserted into the sample measurement device 110. Therefore, the sample measurement device 110 can recognize the insertion of the biosensor strip 120 and the contact of the sample based on the response signal received from the biosensor strip 120, and measure the sample.

[0052] The biosensor strip 120 as described above may be composed of a lower plate 130, a middle plate 140 and an upper plate 150. Figure 3 More specifically, the biosensor strip 120 may include a Figure 4 The electrode structure shown includes a lower plate 130, a middle plate 140 disposed on the upper portion of the lower plate 130 and including a sample insertion path 141 for inserting an enzyme compound that reacts with the sample, and an upper plate 150 disposed on the upper portion of the middle plate 140 and including an insertion port 151 for inserting the sample into the sample insertion path 141 and an exhaust port 152 for exhausting air.

[0053] The sample insertion flow path 141 is self-evident to those skilled in the art, and thus a detailed description thereof is omitted.

[0054] The enzyme compound 142 may include an enzyme for selectively reacting glucose contained in a sample, a polymer for attaching the enzyme to an electrode disposed on the lower plate 130 , and a catalyst for promoting the reaction of the enzyme with glucose.

[0055] At this time, the catalyst may include 1 to 10% of at least one of ferrocene, ferrocene derivatives, quinone, quinone derivatives, hexamethyleneruthenium (III) chloride, Prussian Blue and ferricyanide (when Prussian Blue is used, the catalyst may contain 0.001% to 5% of Prussian Blue), the enzyme may include 0.01 to 10% by weight of at least one of glucose oxidase and glucose dehydrogenase (GDH; glucose dehydrogenase), and the polymer may include 1 to 30 units of at least one of chitosan, PVP, Nafion, polyethylene glycol, polyvinyl pyrrolidone, polyvinyl alcohol, agarose and trehalose.

[0056] The lower plate 130 may be formed with an electrode structure including a strip identification electrode 131 , a sample identification electrode 132 , an identification reference electrode 133 , a working electrode 134 , a working reference electrode 135 , a blank electrode 136 , and spare electrodes 137 , 138 .

[0057] The strip recognition electrode 131 can be used to recognize whether the biosensor strip 120 is inserted into the sample measurement device 110. As an example, a strip recognition signal is applied from the sample measurement device 110 through the strip recognition electrode 131 and the recognition reference electrode 133, and a response signal in response thereto can be provided from the biosensor strip 120 to the sample measurement device 110 through the strip recognition electrode 131 and the recognition reference electrode 133. Therefore, the sample measurement device 110 can recognize whether the biosensor strip 120 is inserted into the sample measurement device 110 using the strip recognition electrode 131. Whether the biosensor strip 120 is inserted into the sample measurement device 110 can be determined based on the strength of the response signal in response to the strip recognition signal or whether the response signal is generated and provided.

[0058] The sample recognition electrode 132 can be used to recognize the sample. More specifically, the sample recognition electrode 132 can be used to recognize whether the sample is in contact with the biosensor strip 120. For example, a sample recognition signal is applied from the sample measurement device 110 through the sample recognition electrode 132 and the recognition reference electrode 133, and a response signal in response thereto can be provided to the sample measurement device 110 from the biosensor strip 120 through the sample recognition electrode 132 and the recognition reference electrode 133. Therefore, the sample measurement device 110 can recognize whether the sample is in contact with the biosensor strip 120 using the sample recognition electrode 132. Whether the sample is in contact with the biosensor strip 120 can be determined based on the strength of the response signal in response to the sample recognition signal or whether the response signal is generated and provided.

[0059] The sample recognition protrusion 132-1 formed in the area of ​​the sample recognition electrode 132 corresponding to the sample insertion flow path 141 of the biosensor strip 120 and the recognition reference protrusion 133-1 formed in the area of ​​the recognition reference electrode 133 corresponding to the sample insertion flow path 141 of the biosensor strip 120 can be spaced apart from each other and arranged opposite to each other in a state of protruding in a direction orthogonal to the direction in which the sample recognition electrode 132 and the recognition reference electrode 133 extend.

[0060] At this time, the identification reference electrode 133 for each of the strip identification electrode 131 and the sample identification electrode 132 may be provided in common to the strip identification electrode 131 and the sample identification electrode 133. That is, the strip identification electrode 131 and the sample identification electrode 132 may share one identification reference electrode 133. Therefore, unlike the case where the strip identification electrode 131 and the sample identification electrode 132 are provided with their own identification reference electrodes, the biosensor strip 120 may be miniaturized.

[0061] The working electrode 134 and the working reference electrode 135 can be used to measure the sample. Specifically, a sample measurement signal is applied from the sample measurement device 110 through the working electrode 134 and the working reference electrode 135, and a response signal about the sample in response to this can be provided to the sample measurement device 110 from the biosensor strip 120 through the working electrode 134 and the working reference electrode 135. Therefore, the sample measurement device 110 can measure the sample by measuring the response signal to the sample using the working electrode 134 and the working reference electrode 135. The sample measurement result can be judged according to the intensity of the response signal in response to the sample measurement signal or whether the response signal is generated and provided. For example, the sample measurement result can show measurement values ​​of multiple stages according to the intensity of the response signal in response to the sample measurement signal.

[0062] The working protrusion 134-1 formed in the area of ​​the working electrode 134 corresponding to the sample insertion flow path 141 of the biosensor strip 120 and the two or more working reference protrusions 135-1, 135-2 formed in the area of ​​the working reference electrode 135 corresponding to the sample insertion flow path 141 can be alternately arranged in a state of protruding in a direction orthogonal to the direction in which the working electrode 134 and the working reference electrode 135 extend.

[0063] As described above, one working protrusion 134-1 is configured between two or more working reference protrusions 135-1 and 135-2 and arranged alternately, and the area of ​​one working protrusion 134-1 is smaller than the area of ​​each of the two or more working reference protrusions 135-1 and 135-2, so that the sample measurement signal and the response signal in response to the sample measurement signal can be accurately measured in the case of sample contact. Therefore, the accuracy of sample measurement can be improved.

[0064] The strip recognition electrode 131 , the sample recognition electrode 132 , the recognition reference electrode 133 , the working electrode 134 , and the working reference electrode 135 may be formed to be parallel to each other and spaced apart and extend in one direction.

[0065] In the electrode structure described above, the strip recognition electrode 131 and the sample recognition electrode 132 can be separately provided so as to electrically work separately with the working electrode 134 and the working reference electrode 135. Therefore, the sample measurement accuracy can be improved by preventing the formation of a double electric layer on the surface of the working electrode 134 and the working reference electrode 135 before measuring the sample.

[0066] The empty electrode 136 is used to make the sample contact the sample insertion flow path 141 with a large area larger than a preset size to improve the branch diffusion defect related to the interface material characteristics, and can be set on one side of the area corresponding to the sample insertion flow path 141 in the lower plate 130.

[0067] The spare electrodes 137 and 138 are spare electrodes in the electrode structure of the biosensor strip 120 and can be used as any of the strip recognition electrode 131, the sample recognition electrode 132, the recognition reference electrode 133, the working electrode 134 and the working reference electrode 135 as needed.

[0068] Although not shown separately, the lower plate 130 formed with the electrode structure may be formed by overlapping a plurality of metal layers. As an example, the lower plate 130 may be implemented as a quadruple structure in which an insulating substrate layer (not shown), a first metal layer (not shown) formed of copper on the insulating substrate layer, a second metal layer (not shown) formed of nickel on the first metal layer, and a third metal layer (not shown) formed of gold on the second metal layer are overlapped. In this case, the electrode structure may be formed on the first metal layer, the second metal layer, the third metal layer, and the fourth metal layer, respectively.

[0069] The sample measuring device 110 can sequentially apply a strip identification signal, a sample identification signal, and a sample measuring signal to the biosensor strip 120 as the sample contacts and flows into the biosensor strip 120, and receive a response signal to each of the strip identification signal, the sample identification signal, and the sample measuring signal, thereby recognizing that the biosensor strip 120 has been inserted and the sample contacts the biosensor strip 120 and measuring the sample.

[0070] The sample measurement result may be provided in the form of multiple stages of measurement values ​​according to the strength of the response signal in response to the sample measurement signal, and different graphic effects may be applied according to the measurement values, and displayed through the display device of the sample measurement device 110 .

[0071] Figure 5 is a flow chart showing a sample measurement method according to an embodiment, Figure 6 It is to illustrate Figure 5 The following sample measurement method is based on the execution subject as shown in the figure. Figures 1 to 4 The sample measurement system 100 is described as a premise.

[0072] Before steps S510 to S530, the sample measurement system 100 (more specifically, the sample collection device 160 ) may be as follows: Figure 6 (a) and (b) use the sample collection part 161 of the sample collection device 160 to collect the subject's saliva and insert it into the compression tube 163 with the filter 162 to remove interfering substances from the saliva sample. Figure 6 (c) inserting the biosensor strip 120 into the sample measuring device 110, and Figure 6 (d) The sample is inserted from the sample collection device 160 into the insertion port 151 of the biosensor strip 120 and flows into the sample insertion flow path 141 of the biosensor strip 120 .

[0073] At this time, the sample inserted and introduced into the biosensor strip 120 may contact the sample insertion path 141 of the biosensor strip 120 over a large area larger than a preset size through the empty electrode 136 disposed at one side of the sample insertion path 141 of the biosensor strip 120 .

[0074] In step S510, the sample measurement system 100 (more precisely, the sample measurement device 110) can use the strip recognition electrode 131 included in the biosensor strip 120 to recognize whether the biosensor strip 120 is inserted into the sample measurement device 110. More specifically, the sample measurement device 110 can apply a strip recognition signal through the strip recognition electrode 131 and the recognition reference electrode 133, and as a response signal in response to this is provided from the biosensor strip 120 to the sample measurement device 110 through the strip recognition electrode 131 and the recognition reference electrode 133, it is recognized and determined whether the biosensor strip 120 is inserted into the sample measurement device 110.

[0075] In step S520, when the biosensor strip 120 is inserted into the sample measuring device 110 (when the biosensor strip 120 is identified in step S510 as being inserted into the sample measuring device 110), the sample measuring system 100 (more precisely, the sample measuring device 110) can use the sample identification electrode 132 included in the biosensor strip to identify whether the sample is in contact with the biosensor strip 120. Specifically, the sample measuring device 110 can apply a sample identification signal through the sample identification electrode 132 and the identification reference electrode 133, and as a response signal in response to this is provided from the biosensor strip 120 to the sample measuring device 110 through the sample identification electrode 132 and the identification reference electrode 133, it can identify and determine whether the sample is in contact with the biosensor strip 120.

[0076] In step S530, the sample measurement system 100 (more precisely, the sample measurement device 110) may, when the sample is in contact with the biosensor strip 120 (when it is identified in step S520 that the sample is in contact with the biosensor strip 120), perform the following steps: Figure 6 (e) applying a sample measurement signal to the working electrode 134 and the working reference electrode 135 included in the biosensor strip 120 to measure a response signal to the sample. More specifically, the sample measurement device 110 may apply the sample measurement signal through the working electrode 134 and the working reference electrode 135, and as a response signal in response to this is provided from the biosensor strip 120 through the working electrode 134 and the working reference electrode 135 to the sample measurement device 100, a response signal in response to the sample measurement signal is measured to measure the sample.

[0077] In particular, since the strip recognition electrode 131 and the sample recognition electrode 132 are configured to work electrically independently from the working electrode 134 and the working reference electrode 135, the step S530 of determining the response signal to the sample can be performed independently of the step S510 of identifying whether the biosensor strip 120 is inserted into the measuring device 100 and the step S520 of identifying whether the sample is in contact with the biosensor strip 120.

[0078] Although the embodiments are described by limited embodiments and figures, a person skilled in the art may make various modifications and variations from the description. For example, even if the described techniques are performed in a different order than the described methods and / or the described systems, structures, devices, circuits, etc. are combined or combined in a different form than the described methods, or are replaced or substituted by other components or equivalents, appropriate results may be achieved.

[0079] Therefore, other implementations, other embodiments and equivalents to the patent claims also fall within the scope of the appended claims.

Claims

1. An electrode structure of a biosensor strip, which is an electrode structure of a biosensor strip used for measuring a sample, comprising: a strip recognition electrode for identifying a sample assay device into which the biosensor strip is inserted; a sample identification electrode for identifying the sample; as well as A working electrode and a working reference electrode are used to measure the sample.

2. The electrode structure of the biosensor strip according to claim 1, characterized in that: The strip recognition electrode and the sample recognition electrode are configured to operate electrically independently from the working electrode and the working reference electrode.

3. The electrode structure of the biosensor strip according to claim 1, characterized in that: The working protrusion formed in the area of ​​the working electrode corresponding to the sample insertion flow path of the biosensor strip and at least two working reference protrusions formed in the area of ​​the working reference electrode corresponding to the sample insertion flow path protrude in a direction orthogonal to the direction in which the working electrode and the working reference electrode extend and are alternately arranged.

4. The electrode structure of the biosensor strip according to claim 1, characterized in that: An identification reference electrode for each of the strip identification electrode and the sample identification electrode is provided to be shared by the strip identification electrode and the sample identification electrode.

5. The electrode structure of the biosensor strip according to claim 4, characterized in that: The sample recognition protrusion formed in the sample recognition electrode in the area corresponding to the sample insertion flow path of the biosensor strip and the recognition reference protrusion formed in the recognition reference electrode in the area corresponding to the sample insertion flow path of the biosensor strip protrude in a direction orthogonal to the direction in which the sample recognition electrode and the recognition reference electrode extend and are arranged opposite to each other.

6. The electrode structure of the biosensor strip according to claim 1, characterized in that: Also included is a blank electrode disposed on one side of the sample insertion flow path of the biosensor strip.

7. The electrode structure of the biosensor strip according to claim 1, characterized in that: The strip recognition electrode, the sample recognition electrode, the working electrode, and the working reference electrode are parallel to and spaced apart from each other and extend in one direction.

8. A biosensor strip for measuring a sample, comprising: a lower plate having an electrode structure including a strip recognition electrode for recognizing a biosensor strip inserted into a sample determination device, a sample recognition electrode for recognizing a sample, and a working electrode and a working reference electrode for determining the sample; a middle plate, disposed on the upper portion of the lower plate and comprising a sample insertion flow path into which an enzyme compound that reacts with the sample is inserted; as well as The upper plate is disposed on the upper portion of the middle plate and includes an insertion port for inserting the sample into the sample insertion flow path.

9. The biosensor strip according to claim 8, characterized in that: In the lower plate, the strip recognition electrode and the sample recognition electrode are arranged to operate electrically independently from the working electrode and the working reference electrode.

10. The biosensor strip according to claim 8, characterized in that: In the lower plate, an identification reference electrode for each of the strip identification electrode and the sample identification electrode is provided to be shared by the strip identification electrode and the sample identification electrode.

11. The biosensor strip according to claim 8, characterized in that: The lower plate further includes a blank electrode disposed on one side of the sample insertion flow path of the biosensor strip.

12. A sample measurement system, comprising: A biosensor strip having an electrode structure including a strip recognition electrode for recognizing a sample measurement device into which the biosensor strip is inserted, a sample recognition electrode for recognizing a sample, and a working electrode and a working reference electrode for measuring the sample; as well as The sample determination device identifies whether the biosensor strip is inserted, identifies whether the sample is in contact with the biosensor strip when the biosensor strip is inserted into the sample determination device, and applies a sample determination signal to the working electrode and the working reference electrode to determine a response signal to the sample when the sample is in contact with the biosensor strip.

13. A sample measurement method, performed by a sample measurement system including a biosensor strip and a sample measurement device, comprising: a step of identifying whether the biosensor strip is inserted into the sample measuring device using a strip identification electrode included in the biosensor strip; When the biosensor strip is inserted into the sample measuring device, a step of using a sample identification electrode included in the biosensor strip to identify whether the sample is in contact with the biosensor strip; as well as When the sample contacts the biosensor strip, a sample measurement signal is applied to a working electrode and a working reference electrode included in the biosensor strip to measure a response signal to the sample.

14. The sample measurement method according to claim 13, characterized in that: In the step of determining a response signal to the sample, the strip identification electrode and the sample identification electrode are configured to operate electrically independently from the working electrode and the working reference electrode, and the steps of identifying whether the biosensor strip is inserted into the sample determination device and identifying whether the sample is in contact with the biosensor strip are performed independently.

15. The sample measurement method according to claim 13, characterized in that: Also includes: The step of using a dummy electrode disposed on one side of the sample insertion flow path of the biosensor strip to make the sample contact with the sample insertion flow path of the biosensor strip to a size greater than a preset size.

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