High-stress-resistance black glutinous rice cultivation method

Abstract

The invention discloses a high-stress-resistance black glutinous rice cultivation method, and belongs to the technical field of crop cultivation. The method specifically comprises the following steps: (1) soaking black glutinous rice seeds in concentrated sulfuric acid for 5-10 minutes, carrying out magnetization treatment, then taking out, disinfecting, washing and airing; (2) putting the treated black glutinous rice seeds into adjusting liquid seeds, and soaking for 20-30 hours; (3) soaking the seeds treated in the step (2) in a nutrient solution, and culturing until germination; and (4) after germination, transplanting the seedlings into a black glutinous rice seedling substrate for continuous cultivation, supplementing light with blue light with the illumination intensity of 800-1000Lx for 6-10 hours every day, and transplanting the seedlings 15-20 days later until the seedlings grow maturely. The black glutinous rice cultivation method is improved, and the survival rate, stress resistance and other characteristics of black glutinous rice seedlings can be effectively improved through cooperation of the specific cultivation process and reagents.

Description

Technical Field

[0001] The invention belongs to the technical field of crop cultivation, and particularly relates to a method for cultivating black glutinous rice with high stress resistance. Background Art

[0002] Black glutinous rice is purple rice. Purple rice is a variety of rice and belongs to the glutinous rice category. Purple rice is very fragrant and sticky when cooked. It is known as purple glutinous rice or "medicine valley" as a tonic. The rice porridge made from purple rice is fragrant, shiny, soft and sticky. Because it is rich in nutrients and has a good tonic effect, it is called "blood-replenishing rice" and "longevity rice". Black glutinous rice is known as "black pearl". It is a rice plant with purple-red seed coat. Because the rice is sticky, it is also called blood glutinous rice. The nutritional value of black glutinous rice is very high. In addition to protein, fat and carbohydrates, it is also rich in calcium, phosphorus, iron, vitamin B1, B2, etc. Black glutinous rice has the effect of nourishing blood and qi. Eating it for a long time can help you stay fit and can also be used to soak in wine.

[0003] Gudao black glutinous rice belongs to the indica glutinous black rice, with a growth period of about 118 days, and is planted as medium and late rice in the middle and lower reaches of the Yangtze River. Black glutinous rice is sweet and warm in nature, enters the spleen, kidney and lung meridians, and has the effects of invigorating qi and strengthening the spleen, promoting body fluid and stopping sweating. In addition, black glutinous rice is high in calcium, has the effect of strengthening bones and teeth, and has high nutritional value. Therefore, how to cultivate black glutinous rice with high resistance is particularly critical. Summary of the invention

[0004] In view of the above-mentioned deficiencies in the prior art, the present invention provides a method for cultivating black glutinous rice with high stress resistance, which can effectively improve the stress resistance of black glutinous rice.

[0005] To achieve the above purpose, the technical solution adopted by the present invention to solve the technical problem is:

[0006] The object of the present invention is to provide a method for cultivating black glutinous rice with high stress resistance, comprising the following steps:

[0007] (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 5 to 10 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them;

[0008] (2) placing the treated black glutinous rice seeds in a conditioning solution and soaking them for 20 to 30 hours, wherein the conditioning solution comprises river mussel shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin in a weight ratio of 0.2 to 1: 0.5 to 1.5: 0.05 to 0.2: 0.5 to 1;

[0009] (3) soaking the seeds treated in step (2) in a nutrient solution and cultivating them until they germinate;

[0010] (4) After germination, the seedlings are transplanted into a black glutinous rice seedling medium for further cultivation, and supplemented with blue light with a light intensity of 800 to 1000 Lx for 6 to 10 hours per day. The seedlings are transplanted after 15 to 20 days of cultivation until they grow to maturity.

[0011] Further, the process of magnetization treatment is:

[0012] The magnetization treatment was performed at a magnetic field strength of 1080-1100 G for 80-90 s, and then paused for 90-100 s. The magnetization treatment was performed at a magnetic field strength of 580-600 G for 60-70 s, and at a magnetic field strength of 1450-1470 G for 120-130 s. The above was one cycle, and the cycle treatment was performed twice in total.

[0013] Furthermore, the weight ratio of river clam shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin is 0.2:0.8:0.2:0.5.

[0014] Furthermore, the nutrient solution includes plant nutrient solution, abscisic acid and methylthioisopentenyl adenosine; wherein the volume ratio of the plant nutrient solution, abscisic acid and methylthioisopentenyl adenosine is 1.2-1.5:0.4-0.5:0.5-0.6.

[0015] Furthermore, the volume ratio of the plant nutrient solution, abscisic acid and methylthioisopentenyl adenosine is 1.2:0.5:0.5.

[0016] Furthermore, the plant nutrient solution is rice plant nutrient solution.

[0017] Furthermore, the culture conditions in step (3) are: 2-4°C, 1000-1500Lx.

[0018] Furthermore, the seedling medium includes humus, vermiculite, ammonium sulfate and modified peat soil; wherein the weight ratio of humus, vermiculite, ammonium sulfate and modified peat soil is 1.2-1.5:1-1.5:0.1-0.5:1-1.5.

[0019] Further, the weight ratio of humus, vermiculite, ammonium sulfate and modified peat soil is 1.2:1.5:0.5:1.5.

[0020] Further, the preparation method of modified peat soil is as follows:

[0021] S1: preparing potassium salt and calcium salt into solutions respectively;

[0022] S2: Spray the potassium salt solution into the peat soil 3 to 5 times. Each time the potassium salt solution is sprayed, leave it to stand and cure at 20 to 40°C for 30 to 60 minutes;

[0023] S3: Spray the calcium salt solution into the peat soil treated by S2 for 3 to 5 times. After each spraying, leave it to stand and cure at 20 to 40°C for 30 to 60 minutes.

[0024] S4: Dry the peat soil treated by S3 at 20-30°C until the moisture content is less than 10%, and then grind it into particles with a particle size not exceeding 2mm.

[0025] Beneficial effects of the present invention:

[0026] 1. There is an innate defense network in the plant body, which can effectively improve the plant body's resistance to diseases and insect pests, as well as in various adverse environments. The present invention uses river clam shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin to prepare a regulating solution, which can effectively activate the innate defense network in the plant body to achieve the purpose of improving the plant body's stress resistance; wherein, 2,6-dichloroisonicotinic acid and betaine are used as the main components for activating the defense network, and 6,7-dihydroxycoumarin and curcumin are used as regulating components to be compatible with 2,6-dichloroisonicotinic acid and river clam shell powder, so that the efficacy is maximized, while the side effects are reduced to an acceptable range.

[0027] 2. In the nutrient solution of the present invention, the plant nutrient solution is used as a nutrient component to provide nutrition for wheat. The compound of abscisic acid and methylthioisopentenyl adenosine can effectively eliminate the negative effects of using a single agent. Among them, abscisic acid effectively promotes the growth of plant roots and overcomes the defect of methylthioisopentenyl adenosine causing excessive growth of plant height. At the same time, the inhibition of abscisic acid on seed germination is effectively eliminated by methylthioisopentenyl adenosine, which can significantly enhance the stress resistance of wheat and has a very significant synergistic effect.

[0028] 3. The present invention also adds modified peat soil to the seedling medium. The peat soil contains organic matter and humus. The peat soil in the present invention is also modified with potassium salt and calcium salt. During the modification, the potassium salt and calcium salt are prepared into a solution, and the potassium salt and calcium salt solution are mixed into the peat soil by spraying. At the same time, the maintenance is carried out at a relatively low temperature. While fully ensuring that potassium ions and calcium ions enter the peat soil, the organic matter of the peat soil will not be destroyed, and the characteristics of the peat soil itself are retained. + It will also improve the charge distribution and hydration film thickness on the surface of peat soil particles, which is conducive to promoting the growth of seedlings; while Ca 2+ It can increase the cohesion between peat soil particles and effectively prevent the loss of water in drilling fluid. The combination of modified peat soil with humus, vermiculite and ammonium sulfate can effectively promote the growth of black glutinous rice seedlings. DETAILED DESCRIPTION

[0029] The specific implementation modes of the present invention are described below so that those skilled in the art can understand the present invention. However, it should be clear that the present invention is not limited to the scope of the specific implementation modes. For those of ordinary skill in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the attached claims, these changes are obvious, and all inventions and creations utilizing the concept of the present invention are protected.

[0030] Example 1

[0031] A method for cultivating black glutinous rice with high stress resistance comprises the following steps:

[0032] (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 10 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them; wherein the magnetization treatment process is as follows:

[0033] The magnetization treatment was carried out under the condition of a magnetic field strength of 1100G for 90s, and then the magnetization treatment was carried out for 100s. The magnetization treatment was carried out under the condition of a magnetic field strength of 600G for 60s, and the magnetization treatment was carried out under the condition of a magnetic field strength of 1450G for 130s. The above was one cycle, and the cycle treatment was carried out twice in total.

[0034] (2) placing the treated black glutinous rice seeds in a conditioning solution and soaking them for 24 hours; wherein the conditioning solution comprises river clam shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin in a weight ratio of 0.2:0.8:0.2:0.5;

[0035] (3) soaking the seeds treated in step (2) in a nutrient solution and culturing them until germination; wherein the nutrient solution comprises a plant nutrient solution, abscisic acid, and methylthioisopentenyl adenosine in a volume ratio of 1.2:0.5:0.5;

[0036] (4) After germination, the seedlings were transplanted into a black glutinous rice seedling medium for further cultivation, supplemented with blue light with a light intensity of 1000 Lx for 10 h every day, and transplanted in the manner recorded in DB43 / T 2098-2021 Technical Specifications for Cultivation of High Resistant Starch Rice after 15 days of seedling cultivation, and subsequently cultivated with water and fertilizer management, pest control, etc. until they grew and matured.

[0037] Example 2

[0038] A method for cultivating black glutinous rice with high stress resistance comprises the following steps:

[0039] (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 10 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them; wherein the magnetization treatment process is as follows:

[0040] The magnetization treatment was performed under the condition of a magnetic field strength of 1080G for 90s, and then the magnetization treatment was performed for 90s. The magnetization treatment was performed under the condition of a magnetic field strength of 600G for 70s, and the magnetization treatment was performed for 130s under the condition of a magnetic field strength of 1470G. The above was one cycle, and the cycle treatment was performed twice in total.

[0041] (2) placing the treated black glutinous rice seeds in a conditioning solution and soaking them for 30 hours; wherein the conditioning solution comprises river clam shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin in a weight ratio of 1:1.5:0.2:0.5;

[0042] (3) soaking the seeds treated in step (2) in a nutrient solution and culturing them until germination; wherein the nutrient solution comprises a plant nutrient solution, abscisic acid and methylthioisopentenyl adenosine in a volume ratio of 1.5:0.5:0.6;

[0043] (4) After germination, the seedlings were transplanted into a black glutinous rice seedling medium for further cultivation, supplemented with blue light with a light intensity of 1000 Lx for 10 h every day, and transplanted in the manner recorded in DB43 / T 2098-2021 Technical Specifications for Cultivation of High Resistant Starch Rice after 20 days of seedling cultivation, and subsequently cultivated with water and fertilizer management, pest control, etc. until they grew and matured.

[0044] Example 3

[0045] A method for cultivating black glutinous rice with high stress resistance comprises the following steps:

[0046] (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 5 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them; wherein the magnetization treatment process is as follows:

[0047] The magnetization treatment was performed under the condition of a magnetic field strength of 1100G for 80s, and then paused for 100s. The magnetization treatment was performed under the condition of a magnetic field strength of 580G for 70s, and the magnetization treatment was performed under the condition of a magnetic field strength of 1470G for 130s. The above was one cycle, and the cycle treatment was performed twice in total.

[0048] (2) placing the treated black glutinous rice seeds in a conditioning solution and soaking them for 30 hours; wherein the conditioning solution comprises river clam shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin in a weight ratio of 1:0.5:0.2:0.5;

[0049] (3) soaking the seeds treated in step (2) in a nutrient solution and culturing them until germination; wherein the nutrient solution comprises a plant nutrient solution, abscisic acid and methylthioisopentenyl adenosine in a volume ratio of 1.2:0.4:0.5;

[0050] (4) After germination, the seedlings were transplanted into a black glutinous rice seedling medium for further cultivation, supplemented with blue light with a light intensity of 1000 Lx for 10 h every day, and transplanted in the manner recorded in DB43 / T 2098-2021 Technical Specifications for Cultivation of High Resistant Starch Rice after 20 days of seedling cultivation, and subsequently cultivated with water and fertilizer management, pest control, etc. until they grew and matured.

[0051] Comparative Example 1

[0052] Compared with Example 1, the magnetization process is missing, and the remaining processes are the same as those in Example 1, which are specifically as follows:

[0053] (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 10 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them; wherein the magnetization treatment process is as follows:

[0054] The magnetization treatment was carried out under the condition of a magnetic field strength of 1100G for 90s, and then the magnetization treatment was carried out for 100s. The magnetization treatment was carried out under the condition of a magnetic field strength of 600G for 60s, and the magnetization treatment was carried out under the condition of a magnetic field strength of 1450G for 130s. The above was one cycle, and the cycle treatment was carried out twice in total.

[0055] (2) placing the treated black glutinous rice seeds in a conditioning solution and soaking them for 24 hours; wherein the conditioning solution comprises river clam shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin in a weight ratio of 0.2:0.8:0.2:0.5;

[0056] (3) soaking the seeds treated in step (2) in a nutrient solution and culturing them until germination; wherein the nutrient solution comprises a plant nutrient solution, abscisic acid, and methylthioisopentenyl adenosine in a volume ratio of 1.2:0.5:0.5;

[0057] (4) After germination, the seedlings were transplanted into a black glutinous rice seedling medium for further cultivation, and supplemented with blue light with a light intensity of 1000 Lx for 10 h every day. The seedlings were transplanted after 15 days of cultivation and cultivated according to conventional methods until they grew and matured.

[0058] Comparative Example 2

[0059] Compared with Example 1, the regulating solution is replaced with a common nutrient solution, and the rest of the process is the same as that of Example 1, as follows:

[0060] (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 10 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them; wherein the magnetization treatment process is as follows:

[0061] The magnetization treatment was carried out under the condition of a magnetic field strength of 1100G for 90s, and then the magnetization treatment was carried out for 100s. The magnetization treatment was carried out under the condition of a magnetic field strength of 600G for 60s, and the magnetization treatment was carried out under the condition of a magnetic field strength of 1450G for 130s. The above was one cycle, and the cycle treatment was carried out twice in total.

[0062] (2) placing the treated black glutinous rice seeds in Yoshida rice nutrient solution and soaking them for 24 hours;

[0063] (3) soaking the seeds treated in step (2) in a nutrient solution and culturing them until germination; wherein the nutrient solution comprises a plant nutrient solution, abscisic acid, and methylthioisopentenyl adenosine in a volume ratio of 1.2:0.5:0.5;

[0064] (4) After germination, the seedlings were transplanted into a black glutinous rice seedling medium for further cultivation, and supplemented with blue light with a light intensity of 1000 Lx for 10 h every day. The seedlings were transplanted after 15 days of cultivation and cultivated according to conventional methods until they grew and matured.

[0065] Comparative Example 3

[0066] Compared with Example 1, the regulating solution lacks curcumin and river mussel shell powder, and the rest of the process is the same as Example 1, as follows:

[0067] (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 10 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them; wherein the magnetization treatment process is as follows:

[0068] The magnetization treatment was carried out under the condition of a magnetic field strength of 1100G for 90s, and then the magnetization treatment was carried out for 100s. The magnetization treatment was carried out under the condition of a magnetic field strength of 600G for 60s, and the magnetization treatment was carried out under the condition of a magnetic field strength of 1450G for 130s. The above was one cycle, and the cycle treatment was carried out twice in total.

[0069] (2) placing the treated black glutinous rice seeds in a conditioning solution and soaking them for 24 hours; the conditioning solution comprises 2,6-dichloroisonicotinic acid and 6,7-dihydroxycoumarin in a weight ratio of 0.8:0.2;

[0070] (3) soaking the seeds treated in step (2) in a nutrient solution and culturing them until germination; wherein the nutrient solution comprises a plant nutrient solution, abscisic acid, and methylthioisopentenyl adenosine in a volume ratio of 1.2:0.5:0.5;

[0071] (4) After germination, the seedlings were transplanted into a black glutinous rice seedling medium for further cultivation, and supplemented with blue light with a light intensity of 1000 Lx for 10 h every day. The seedlings were transplanted after 15 days of cultivation and cultivated according to conventional methods until they grew and matured.

[0072] Comparative Example 4

[0073] Compared with Example 1, the nutrient solution only contains plant nutrient solution and methylthioisopentenyl adenosine, and the rest of the process is the same as Example 1, specifically as follows:

[0074] (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 10 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them; wherein the magnetization treatment process is as follows:

[0075] The magnetization treatment was carried out under the condition of a magnetic field strength of 1100G for 90s, and then the magnetization treatment was carried out for 100s. The magnetization treatment was carried out under the condition of a magnetic field strength of 600G for 60s, and the magnetization treatment was carried out under the condition of a magnetic field strength of 1450G for 130s. The above was one cycle, and the cycle treatment was carried out twice in total.

[0076] (2) placing the treated black glutinous rice seeds in a conditioning solution and soaking them for 24 hours; the conditioning solution comprises 2,6-dichloroisonicotinic acid and 6,7-dihydroxycoumarin in a weight ratio of 0.8:0.2;

[0077] (3) soaking the seeds treated in step (2) in a nutrient solution and culturing them until germination; wherein the nutrient solution comprises a plant nutrient solution and methylthioisopentenyl adenosine in a volume ratio of 1.2:0.5;

[0078] (4) After germination, the seedlings were transplanted into a black glutinous rice seedling medium for further cultivation, and supplemented with blue light with a light intensity of 1000 Lx for 10 h every day. The seedlings were transplanted after 15 days of cultivation and cultivated according to conventional methods until they grew and matured.

[0079] 300 seeds were taken and cultivated for 30 to 40 days according to the methods described in Examples 1 to 3 and Comparative Examples 1 to 4, and the germination rate, survival rate, plant height and other index parameters of the black glutinous rice seeds were tested. The results are shown in Table 1.

[0080] Table 1 Black glutinous rice index detection

[0081]

[0082] As can be seen from the data in Table 1, the germination rate, survival rate, plant height and other indicators of Comparative Examples 1 to 4 cannot be compared with the technical solutions described in Examples 1 to 3 of the present application due to the replacement and modification of the cultivation methods and reagents used in the cultivation process. This shows that only with the cooperation of the method and reagents of the present invention can black glutinous rice seedlings with excellent performance be cultivated.

[0083] Finally, it should be noted that the above specific implementation methods are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to examples, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. A method for cultivating black glutinous rice with high stress resistance, characterized in that: The following steps are involved: (1) Soaking black glutinous rice seeds in concentrated sulfuric acid for 5 to 10 minutes, performing magnetization treatment, and then taking them out for disinfection, washing them, and drying them; (2) placing the treated black glutinous rice seeds in a conditioning solution and soaking them for 20 to 30 hours, wherein the conditioning solution comprises river mussel shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin in a weight ratio of 0.2 to 1: 0.5 to 1.5: 0.05 to 0.2: 0.5 to 1; (3) soaking the seeds treated in step (2) in a nutrient solution and cultivating them until they germinate; (4) After germination, the seedlings are transplanted into a black glutinous rice seedling medium for further cultivation, and supplemented with blue light with a light intensity of 800 to 1000 Lx for 6 to 10 hours per day. The seedlings are transplanted after 15 to 20 days of cultivation until they grow to maturity.

2. The method for cultivating black glutinous rice with high stress resistance according to claim 1, characterized in that: The process of magnetization is: The magnetization treatment was performed at a magnetic field strength of 1080-1100 G for 80-90 s, and then paused for 90-100 s. The magnetization treatment was performed at a magnetic field strength of 580-600 G for 60-70 s, and at a magnetic field strength of 1450-1470 G for 120-130 s. The above was one cycle, and the cycle treatment was performed twice in total.

3. The method for cultivating black glutinous rice with high stress resistance according to claim 1, characterized in that: The weight ratio of river clam shell powder, 2,6-dichloroisonicotinic acid, 6,7-dihydroxycoumarin and curcumin is 0.2:0.8:0.2:0.

5.

4. The method for cultivating black glutinous rice with high stress resistance according to claim 1, characterized in that: The nutrient solution includes plant nutrient solution, abscisic acid and methylthioisopentenyl adenosine; wherein the volume ratio of the plant nutrient solution, abscisic acid and methylthioisopentenyl adenosine is 1.2-1.5:0.4-0.5:0.5-0.

6.

5. The method for cultivating black glutinous rice with high stress resistance according to claim 4, characterized in that: The volume ratio of plant nutrient solution, abscisic acid and methylthioisopentenyl adenosine is 1.2:0.5:0.

5.

6. The method for cultivating black glutinous rice with high stress resistance according to claim 1, characterized in that: The culture conditions in step (3) are: 2-4°C, 1000-1500Lx.

7. The method for cultivating black glutinous rice with high stress resistance according to claim 1, characterized in that: The seedling medium includes humus, vermiculite, ammonium sulfate and modified peat soil; wherein the weight ratio of the humus, vermiculite, ammonium sulfate and modified peat soil is 1.2-1.5:1-1.5:0.1-0.5:1-1.

5.

8. The method for cultivating black glutinous rice with high stress resistance according to claim 7, characterized in that: The weight ratio of humus, vermiculite, ammonium sulfate and modified peat soil is 1.2:1.5:0.5:1.

5.

9. The method for cultivating black glutinous rice with high stress resistance according to claim 7, characterized in that: The preparation method of modified peat soil is as follows: S1: preparing potassium salt and calcium salt into solutions respectively; S2: Spray the potassium salt solution into the peat soil 3 to 5 times. Each time the potassium salt solution is sprayed, leave it to stand and cure at 20 to 40°C for 30 to 60 minutes; S3: Spray the calcium salt solution into the peat soil treated by S2 for 3 to 5 times. After each spraying, leave it to stand and cure at 20 to 40°C for 30 to 60 minutes. S4: Dry the peat soil treated by S3 at 20-30°C until the moisture content is less than 10%, and then grind it into particles with a particle size not exceeding 2mm.

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