Method for increasing collagen content in donkey hide

Abstract

The invention provides a method for increasing the collagen content in donkey skin, and belongs to the technical field of feed processing, and the method comprises the following steps: S1, selecting 7-8-month-old female donkeys with similar body mass and fetal number, and carrying out empty stomach for 12 hours; s2, feeding each donkey in a semi-open single fence for 130 days; s3, feeding two times of concentrated feed and three times of coarse feed every day, and freely drinking water; s4, weighing with an empty stomach on the 130th day, and calculating the feed intake of the concentrated feed; the method is used for solving the technical problems that a research method for increasing the collagen content in the donkey hide does not exist in the prior art, and the collagen content in the donkey hide is not considered in the current nutritional requirement parameters.

Description

Technical Field

[0001] The invention belongs to the technical field of feed processing, and in particular relates to a method for increasing the collagen content in donkey skin. Background Art

[0002] The skin is the largest organ in the body and is also an important barrier that protects the body from external damage. The barrier function of the skin requires the skin to have a certain mechanical strength and toughness to prevent damage to the skin caused by external impact. The collagen fibers contained in the dermis are an important source of the skin's mechanical strength and toughness. Therefore, the skin contains a large amount of collagen fibers, which can account for 70-80% of the dry weight of the skin. Collagen fibers are mainly composed of collagen, which determines the high content of collagen in the skin.

[0003] The main component of donkey skin tissue is protein, which accounts for about 80%, of which collagen accounts for 55.4% of the total protein. There is no research method for increasing the collagen content in donkey skin in the existing technology, and the current nutritional requirement parameters do not take into account the collagen content in donkey skin. Summary of the invention

[0004] (I) Purpose of the invention

[0005] In order to overcome the above disadvantages, the object of the present invention is to provide a method for increasing the collagen content in donkey skin to solve the above technical problems.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the technical solutions provided by this application are as follows:

[0008] A method for increasing the collagen content in donkey skin comprises the following steps:

[0009] S1: Select 7-8 month old female donkeys with similar body weight and parity, fasting for 12 hours;

[0010] S2: Each donkey was raised in a semi-open single stall and fed for 130 days;

[0011] S3: Feeding 2 times of concentrate and 3 times of roughage per day, wherein concentrate is fed at 1.5% of body weight, the concentrate is the basic diet, the basic diet mainly includes corn, soybean meal, rice bran meal, wheat, second meal, stone powder, calcium bicarbonate, salt, premix, glycine, vitamin C and residual feed, the roughage is fed at 2.0% of body weight, the roughage is straw, and drinking water is free;

[0012] S4: Weigh the pigs on an empty stomach on the 130th day and calculate the concentrate feed intake.

[0013] Preferably, the formula ingredients of the basic daily feed specifically include: 30% corn, 21% soybean meal, 14% rice bran meal, 12% wheat, 11.8% second-grade flour, 4% stone powder, 1.6% calcium bicarbonate, 0.6% salt, 2% premix, 1.8% glycine, and 0.1% vitamin C.

[0014] Preferably, the purity of the premix is ​​2%.

[0015] Preferably, the purity of the glycine is 99%.

[0016] Preferably, the purity of the vitamin C is 95%.

[0017] Preferably, the basal diet also includes zinc sulfate.

[0018] Beneficial effects:

[0019] The present invention can effectively increase the content of collagen in donkey skin by adding glycine and vitamin C on the basis of basic diet. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that these descriptions are exemplary only and are not intended to limit the scope of the present invention. In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.

[0021] The present invention provides a method for increasing the collagen content in donkey skin, comprising the following steps:

[0022] S1: Select 7-8 month old female donkeys with similar body weight and parity, fasting for 12 hours;

[0023] S2: Each donkey was raised in a semi-open single stall and fed for 130 days;

[0024] S3: fed 2 times of concentrated feed and 3 times of roughage per day, with free access to water;

[0025] S4: Weigh on an empty stomach on the 130th day and calculate the concentrate feed intake;

[0026] The experimental donkeys fasted for 12 hours the day before the start of the experiment, and their body weight was measured uniformly in repeated units. Their body weight was weighed on an empty stomach on the 130th day of the experimental period, and their concentrate feed intake was calculated.

[0027] Average daily feed intake (ADFI) = daily concentrate feed intake + daily roughage feed intake Average daily weight gain (ADG) = (average final test body - average initial test body) / test days Feed-to-weight ratio (F / G) = average daily feed intake / average daily weight gain

[0028] The concentrate feed is fed at 1.5% of body weight, and the concentrate feed is the basic diet, which mainly includes corn, soybean meal, rice bran meal, wheat, second meal, stone powder, calcium bicarbonate, salt, premix, glycine, vitamin C and residual feed. The roughage is grain straw, which is fed at 2.0% of body weight.

[0029] The formula ingredients of the basic daily feed specifically include 30% corn, 21% soybean meal, 14% rice bran meal, 12% wheat, 11.8% second-grade flour, 4% stone powder, 1.6% calcium bicarbonate, 0.6% salt, 2% premix, 1.8% glycine, and 0.1% vitamin C.

[0030] Zinc sulfate is also included in the basal diet. Zinc sulfate improves zinc deposition in the skin and promotes collagen deposition in the donkey's neck skin.

[0031] Among them, the purity of the premix is ​​2%, the purity of glycine is 99%, and the purity of vitamin C is 95%.

[0032] Example 1

[0033] Fourteen healthy Texas donkeys aged 7-8 months with similar body weight, age and parity were randomly selected and divided into two treatment groups according to the principle of similar body weight, with 7 replicates in each treatment group and one donkey in each replicate. The experimental period was 130 days.

[0034] Each donkey was raised in a semi-open single stall and fed with concentrated feed twice and grass feed three times a day; the concentrated feed was fed at 1.5% of body weight, and the roughage was straw at 2.0% of body weight; and the donkeys were given free access to water.

[0035] At the end of the experiment, 3 donkeys were selected from each group for slaughter. After slaughter, the donkey skin with a length and width of about 4 cmX1.5 cm on the neck and back was cut with surgical scissors, divided into 6 parts and placed in 2 mL cryotubes, temporarily stored in a liquid nitrogen tank, and then stored in a -80 ° C refrigerator for use. The content of type I and type III collagen was determined using the corresponding kit (Nanjing Jiancheng Bioengineering Institute, type I collagen kit serial number H142-1-2, type III collagen kit serial number H144-1-2), and the determination steps were carried out according to the instructions. The detection instrument was a Multiskan MK3 microplate reader.

[0036] As shown in Table 2 , the total amount of collagen in group B was higher than that in group A, indicating that the addition of glycine can increase the content of collagen.

[0037] Table 1 Experimental design

[0038]

[0039] Table 2 Effect of glycine on collagen content in donkey skin and donkey meat of Texas donkey

[0040]

[0041]

[0042] Example 2

[0043] Fourteen healthy Texas donkeys aged 7-8 months with similar body weight, age and parity were randomly selected and divided into two treatment groups according to the principle of similar body weight, with 7 replicates in each treatment group and one donkey in each replicate. The experimental period was 130 days.

[0044] Each donkey was raised in a semi-open single stall and fed with concentrated feed twice and grass feed three times a day; the concentrated feed was fed at 1.5% of body weight, and the roughage was straw at 2.0% of body weight; and the donkeys were given free access to water.

[0045] At the end of the experiment, 3 donkeys were selected from each group for slaughter. After slaughter, the donkey skin with a length and width of about 4 cmX1.5 cm on the neck and back was cut with surgical scissors, divided into 6 parts and placed in 2 mL cryotubes, temporarily stored in a liquid nitrogen tank, and then stored in a -80 ° C refrigerator for use. The content of type I and type III collagen was determined using the corresponding kit (Nanjing Jiancheng Bioengineering Institute, type I collagen kit serial number H142-1-2, type III collagen kit serial number H144-1-2), and the determination steps were carried out according to the instructions. The detection instrument was a Multiskan MK3 microplate reader.

[0046] As shown in Table 4, the collagen content of group B was higher than that of group A, indicating that the addition of vitamin C to the basal diet can increase the collagen content.

[0047] Table 3 Experimental design

[0048]

[0049] Table 4 Effect of vitamin C on collagen content in donkey skin and donkey meat of Texas donkey

[0050]

[0051] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0052] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for increasing the collagen content in donkey skin, characterized in that: The steps include: S1: Select 7-8 month old female donkeys with similar body weight and parity, fasting for 12 hours; S2: Each donkey was raised in a semi-open single stall and fed for 130 days; S3: Feeding 2 times of concentrate and 3 times of roughage per day, wherein concentrate is fed at 1.5% of body weight, the concentrate is the basic diet, the basic diet mainly includes corn, soybean meal, rice bran meal, wheat, second meal, stone powder, calcium bicarbonate, salt, premix, glycine, vitamin C and residual feed, the roughage is fed at 2.0% of body weight, the roughage is straw, and drinking water is free; S4: Weigh the pigs on an empty stomach on the 130th day and calculate the concentrate feed intake.

2. A method for increasing the collagen content in donkey skin according to claim 1, characterized in that: The formula ingredients of the basic daily feed specifically include: 30% corn, 21% soybean meal, 14% rice bran meal, 12% wheat, 11.8% second-grade flour, 4% stone powder, 1.6% calcium bicarbonate, 0.6% salt, 2% premix, 1.8% glycine, and 0.1% vitamin C.

3. A method for increasing the collagen content in donkey skin according to claim 1, characterized in that: The purity of the premix is ​​2%.

4. A method for increasing the collagen content in donkey skin according to claim 1, characterized in that: The purity of the glycine is 99%.

5. A method for increasing the collagen content in donkey skin according to claim 1, characterized in that: The purity of the vitamin C is 95%.

6. A method for increasing the collagen content in donkey skin according to claim 1, characterized in that: Zinc sulfate was also included in the basal diet.

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