Musca domestica larva attractant, musca domestica larva trapping and killing device, preparation method and application

Abstract

The invention provides a housefly larva attractant and a preparation method and application thereof, and belongs to the technical field of biological control. The invention relates to an attractant for housefly larvae and a preparation method of the attractant, and aims to provide an efficient and environment-friendly housefly pest prevention and control solution. The musca domestica larva attractant can specifically attract musca domestica larvae, can be applied to factory-like efficient production of maggots, is developed into a phagostimulant, and is beneficial to improving the feed feeding rate and the feed nutrition conversion efficiency in musca domestica larva breeding production, saving the breeding cost, improving the quality of the maggots and improving the production conversion efficiency and the economic benefits; and the composition can be combined with any reagent with insecticidal effect to achieve the effect of intensively trapping and killing the housefly larvae.

Description

Technical Field

[0001] The invention belongs to the technical field of biological control, and in particular relates to a housefly larvae attractant, a housefly larvae trapping and killing device, and a preparation method and application thereof. Background Art

[0002] The housefly (Musca domestica) belongs to the family Muscidae in the order Diptera of the class Insecta. It is a holometabolous insect that goes through four different life stages: egg, larva, pupa and adult. It has a short life cycle, strong reproductive ability, omnivorous diet and strong adaptability to the environment. It is a widely distributed urban health pest in the world. It likes to crawl and forage in dirty environments such as human and animal feces, urine, and garbage. It is an important disseminator of disease pathogens such as various bacteria, viruses and parasites.

[0003] Housefly larvae are rich in nutrients such as protein and amino acids. Large-scale factory-based fly maggot farming can provide a cheap and high-quality protein source for economic products such as livestock feed. In addition, fly maggots play a very important purification role in the ecological cycle system, and can efficiently convert feces, domestic garbage, fermented crop straw, etc. into organic matter. Improving the efficiency of fly maggot farming has far-reaching environmental and ecological benefits and social and economic value.

[0004] Feeding is one of the important life activities of housefly larvae, which is of great significance to the vitality of adults, the maintenance of population size and the ability to spread pathogens. In addition, housefly larvae have weak mobility and poor migration, which is an effective stage for integrated pest control. Manipulating their olfactory recognition and chemical perception through behavioral regulators such as attractants and repellents, and then killing them in a concentrated manner is one of the green and environmentally friendly strategies for pest control. However, the existing housefly attractants are all targeted at adults, and there are no reports on attractants for larvae, and there are no reports on trapping and killing devices for housefly larvae. Summary of the invention

[0005] The purpose of the present invention is to provide a housefly larvae attractant, a housefly larvae trapping and killing device, and a preparation method and application. The housefly larvae attractant of the present invention can specifically lure housefly larvae. The housefly larvae trapping and killing device of the present invention can achieve the luring effect of the attractant and achieve the purpose of trapping and killing housefly larvae.

[0006] The invention provides a housefly larvae attractant, comprising an active ingredient and a solvent, wherein the active ingredient comprises methyl thiobutyrate and / or ethyl n-hexanoate.

[0007] Preferably, the solvent comprises paraffin oil.

[0008] Preferably, the mass concentration of the active ingredient in the housefly larvae attractant includes 10 -3 mg / μL~10 - 2 mg / μL.

[0009] The present invention also provides a method for preparing the housefly larvae attractant described in the above technical solution, comprising the following steps:

[0010] The active ingredient and the solvent are mixed to obtain a housefly larvae attractant.

[0011] The present invention also provides the use of the housefly larvae attractant described in the above technical solution or the housefly larvae attractant prepared by the preparation method described in the above technical solution in concentrated trapping and killing of housefly larvae and / or in luring housefly larvae.

[0012] The present invention also provides a product containing the housefly larvae attractant. The product comprises the housefly larvae attractant and auxiliary materials, and the auxiliary materials comprise feed.

[0013] The present invention also provides a housefly attractant, which comprises an attractant and an agent with insecticidal effect; the attractant comprises the housefly larvae attractant described in the above technical solution or the housefly larvae attractant prepared by the preparation method described in the above technical solution.

[0014] Preferably, the active ingredients of the insecticide include bifenthrin and / or triflumuron.

[0015] The present invention also provides a housefly larvae trapping and killing device, comprising a tube body (5) and a sealing cover (6) arranged at the tube mouth of the tube body; the tube body is provided with an opening (7) for releasing the larvae; the sealing cover is made of agarose gel, and a filter paper (8) for adding an attractant is arranged inside the sealing cover; and an insecticidal trap (9) for adding an insecticidal agent is arranged at a position of the tube body close to the sealing cover.

[0016] The present invention also provides the use of the housefly larvae attractant described in the above technical solution or the housefly larvae attractant prepared by the preparation method described in the above technical solution and / or the housefly larvae trapping and killing device described in the above technical solution in urban health pest control.

[0017] The present invention provides a housefly larvae attractant. The housefly larvae attractant of the present invention can specifically attract housefly larvae, can be applied to the factory production of fly maggots, and is developed into an attractant, which is beneficial to improving the feed intake rate and feed nutrient conversion efficiency in the breeding production of housefly larvae, saving breeding costs, improving the quality of fly maggots, and improving production conversion efficiency and economic benefits, thereby providing theoretical guidance and basis for the factory production of fly maggots.

[0018] The present invention also provides a housefly larvae trapping device. Urban health pests are mostly within the range of human activities. Large-scale and wide-range application of insecticidal agents may affect the safety of humans and livestock and the safety of the ecological environment. The use of attractants can help improve the safety of urban health pest control. The application of the present invention can indirectly enhance the effect of chemical control, help increase the effectiveness and reduce the amount of insecticidal agents and reduce the application range; it can also directly attract and kill them in a concentrated manner, enhance the effects and functions of other control methods, and achieve green, environmentally friendly and pollution-free pest control. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 A schematic diagram of a housefly larvae attracting device provided by the present invention;

[0021] Figure 2 A comparison chart of the preference index of housefly larvae for different ester compounds provided by the present invention;

[0022] Figure 3 A schematic diagram of a housefly larvae trapping and killing device provided by the present invention;

[0023] Figure 4 This is a comparison chart of the housefly larvae selection preference index and trapping index of different ester compounds provided by the present invention. DETAILED DESCRIPTION

[0024] The present invention provides a housefly larvae attractant, comprising an active ingredient and a solvent, wherein the active ingredient comprises methyl thiobutyrate and / or ethyl n-hexanoate. In the present invention, the solvent preferably comprises paraffin oil. In the present invention, the mass concentration of the active ingredient in the housefly larvae attractant is 10 -3 mg / μL~10 -2 mg / μL, more preferably 10 -3 mg / μL.

[0025] In the present invention, when the active ingredients include methyl thiobutyrate and ethyl hexanoate, the mass ratio of methyl thiobutyrate to ethyl hexanoate is preferably 1 to 9:1 to 9, more preferably 1:1 or 3:7. In the present invention, when the active ingredients include methyl thiobutyrate and ethyl hexanoate, the total mass concentration of methyl thiobutyrate and ethyl hexanoate in the housefly larvae attractant is preferably 2×10 -3In the present invention, when the active ingredient is methyl thiobutyrate or ethyl hexanoate, the mass concentration of methyl thiobutyrate in the housefly larvae attractant is preferably 10 -3 mg / μL; the mass concentration of ethyl hexanoate in the housefly larvae attractant is preferably 10 -3 mg / μL.

[0026] The present invention also provides a method for preparing the housefly larvae attractant described in the above technical solution, comprising the following steps:

[0027] The active ingredient and the solvent are mixed to obtain a housefly larvae attractant.

[0028] The present invention mixes an active ingredient and a solvent to obtain a housefly larvae attractant. In the present invention, the solvent preferably includes paraffin oil. In the present invention, when the active ingredient includes methyl thiobutyrate and ethyl hexanoate, the mass ratio of methyl thiobutyrate to ethyl hexanoate is preferably 1 to 9:1 to 9, more preferably 1:1 or 3:7. In the present invention, when the active ingredient includes methyl thiobutyrate and ethyl hexanoate, the total mass concentration of methyl thiobutyrate and ethyl hexanoate in the housefly larvae attractant is preferably 2×10 -3 In the present invention, when the active ingredient is methyl thiobutyrate or ethyl hexanoate, the mass concentration of methyl thiobutyrate in the housefly larvae attractant is preferably 10 -3 mg / μL; the mass concentration of ethyl hexanoate in the housefly larvae attractant is preferably 10 -3 mg / μL.

[0029] The present invention also provides the use of the housefly larvae attractant described in the above technical solution or the housefly larvae attractant prepared by the preparation method described in the above technical solution in concentrated trapping and killing of housefly larvae and / or in luring housefly larvae.

[0030] The present invention also provides a product containing the housefly larvae attractant, the product comprising the housefly larvae attractant and auxiliary materials, the auxiliary materials comprising feed. In the present invention, the attractant can be used as an attractant to increase the feeding rate and feed nutrient conversion efficiency of housefly larvae and the like as industrial high-protein economic beneficial insects, save breeding costs, improve the quality of fly maggots, and improve production conversion efficiency and economic benefits.

[0031] The present invention also provides a housefly attractant, which comprises an attractant and an agent with insecticidal effect; the attractant comprises the housefly larvae attractant described in the above technical solution or the housefly larvae attractant prepared by the preparation method described in the above technical solution.

[0032] In the present invention, the active ingredient of the agent having an insecticidal effect is preferably any agent having an insecticidal effect, and more preferably includes bifenthrin and / or triflumuron.

[0033] In the present invention, when the active ingredient of the insecticidal agent is bifenthrin or triflumuron, the solvent for preparing the insecticidal agent preferably includes dimethyl sulfoxide. In the present invention, the active ingredient of the insecticidal agent and the solvent are preferably mixed to obtain an insecticidal agent suitable for use in combination with a housefly larvae attractant.

[0034] The present invention also provides a housefly larvae trapping and killing device, comprising a tube body (5) and a sealing cover (6) arranged at the tube mouth of the tube body; the tube body is provided with an opening (7) for releasing the larvae; the sealing cover is made of agarose gel, and a filter paper (8) for adding an attractant is arranged inside the sealing cover; and an insecticidal trap (9) for adding an insecticidal agent is arranged at a position of the tube body close to the sealing cover.

[0035] In the present invention, the insecticidal trap (9) and the pipe body (5) are preferably connected by gluing with a hot melt glue gun.

[0036] In the present invention, the active ingredients of the insecticide preferably include bifenthrin and / or triflumuron.

[0037] In the present invention, it is preferred that the active ingredient of the insecticidal agent and a solvent are mixed to obtain an insecticidal agent suitable for a housefly larvae trap.

[0038] In the present invention, the opening (7) is preferably covered with a sealing film during use.

[0039] In the present invention, the agarose gel is preferably prepared from 1% agarose solution.

[0040] A 1% agarose solution is poured into a cylinder with the same inner diameter as the tube body (5) of the housefly larvae trapping device to shape the cylinder, thereby obtaining a cylindrical agarose gel block as a sealing cover.

[0041] The present invention also provides the use of the housefly larvae attractant described in the above technical solution or the housefly larvae attractant prepared by the preparation method described in the above technical solution and / or the housefly larvae trapping and killing device described in the above technical solution in urban health pest control.

[0042] In order to further illustrate the present invention, the housefly larvae attractant, housefly larvae trapping and killing device, preparation method and application provided by the present invention are described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention. Unless otherwise specified, the reagents in the embodiments of the present invention can be purchased through commercial channels.

[0043] Example 1

[0044] Using paraffin oil as solvent, weigh 100 mg of methyl thiobutyrate original drug, dilute to a total volume of 1000 μL, mix well, and obtain a concentration of 10 -1 mg / μL methyl thiobutyrate solution. Then measure 10 -1 10 μL of methyl thiobutyrate solution with a concentration of 10 mg / μL was added to make the total volume 1000 μL and mixed evenly to obtain a methyl thiobutyrate solution with a concentration of 10 -3 mg / μL of housefly larvae attractant.

[0045] Example 2

[0046] Using paraffin oil as solvent, weigh 100 mg of ethyl hexanoate original drug, dilute to a total volume of 1000 μL, mix well, and obtain a concentration of 10 -1 mg / μL ethyl hexanoate solution. Then measure 10 -1 10 μL of ethyl hexanoate solution with a concentration of 10 mg / μL was added to make the total volume 1000 μL and mixed evenly to obtain a 10 mg / μL ethyl hexanoate solution. -3 mg / μL of housefly larvae attractant.

[0047] Example 3

[0048] Using paraffin oil as solvent, weigh 100 mg of methyl thiobutyrate original drug, dilute to a total volume of 1000 μL, mix well, and obtain a concentration of 10 -1 mg / μL methyl thiobutyrate solution. Then measure 10 -1 10 μL of methyl thiobutyrate solution with a concentration of 10 mg / μL was added, and the total volume was adjusted to 100 μL. The mixture was mixed evenly to obtain a methyl thiobutyrate solution with a concentration of 10 -2 mg / μL of housefly larvae attractant.

[0049] Example 4

[0050] Using paraffin oil as solvent, weigh 100 mg of ethyl hexanoate original drug, dilute to a total volume of 1000 μL, mix well, and obtain a concentration of 10 -1 mg / μL ethyl hexanoate solution. Then measure 10 -1 10 μL of ethyl hexanoate solution with a concentration of 10 mg / μL was added to make the total volume 100 μL and mixed evenly to obtain a 10 mg / μL ethyl hexanoate solution. -2 mg / μL of housefly larvae attractant.

[0051] Example 5

[0052] Using paraffin oil as solvent, weigh 100 mg of methyl thiobutyrate and ethyl hexanoate, and dilute them to a total volume of 1000 μL to obtain a concentration of 10 -1 mg / μL of methyl thiobutyrate and ethyl hexanoate solution. Then measure 10 -1 10 μL of each of the methyl thiobutyrate and ethyl hexanoate solutions at 1000 mg / μL were mixed and the total volume was adjusted to 1000 μL. The mixture was vortexed and mixed to obtain a total mass concentration of 2×10 -3 mg / μL, and the mass ratio of methyl thiobutyrate to ethyl hexanoate was 1:1. Similarly, the attractants with the mass ratio of methyl thiobutyrate to ethyl hexanoate were 9:1, 1:9, 7:3, and 3:7, and the total mass concentration was 2×10 -3 mg / μL.

[0053] Example 6

[0054] Weigh 100 μL of methyl thiobutyrate and / or 100 mg of ethyl hexanoate and add them to 100 mL of distilled water solution containing 0.1% Tween-80. At the same time, weigh 100 g of fresh wheat bran and 5 g of milk powder and mix them evenly with the above aqueous solution to prepare a housefly larvae attractant feed.

[0055] Application Example 1

[0056] Two-way selection and attraction experiment for housefly larvae

[0057] 1. Preparation of the Experimental Apparatus for Attracting Housefly Larvae

[0058] A custom acrylic round tube (3) with a total length of 20 cm, an outer diameter of 2.5 cm, and an inner diameter of 2.1 cm is made, and an opening (2) with a length of 2 cm and a width of 1 cm is opened in the middle of the round tube;

[0059] Pour 1% agarose solution (Agarose, full gold) into a cylinder with an inner diameter of 2.1 cm and a height of 1 cm to shape it, thereby obtaining a cylindrical agarose gel block (4);

[0060] The obtained cylindrical agarose gel block was inserted into both sides of the custom acrylic round tube to close the openings on both sides of the custom PE tube to obtain a housefly larvae attracting device, such as Figure 1 shown.

[0061] 2. Preparation of ester compound carrier

[0062] Clean filter paper is cut into several square filter paper pieces (1) with a side length of 1 cm and used as carriers for loading ester compounds.

[0063] 3. Pre-starvation treatment of housefly larvae for experiments

[0064] The housefly larvae from the late second to early third instar were picked out from the conventional larval culture medium, cleaned, transferred to a culture dish containing 1% agarose gel to move freely, and used in the experiment after being starved for 2 hours.

[0065] 4. Experiment on two-way selection of housefly larvae

[0066] The experiment was conducted in the insect breeding room 319 of the Nankai University Student Testing Building with a temperature of 26±1°C, a relative humidity of 50±3%, and a photoperiod of 14 h light / 10 h dark.

[0067] The control group was paraffin oil, and the experimental group was methyl thiobutyrate, ethyl hexanoate, and a combination of the two ester compounds in a mass ratio of 1:1, 9:1, 1:9, 7:3, and 3:7.

[0068] Control group: Place a cut filter paper (1) on the agarose gel block formed in the housefly larvae attracting device, use a pipette to draw 10 μL of paraffin oil solvent and drop it on the filter paper, then insert the agarose gel block with the filter paper (4) back into the two sides of the acrylic tube. Figure 1 As shown, 10 housefly larvae were released from the middle opening (2) each time, and the number of larvae on both sides of the acrylic tube was observed. The number of larvae was recorded with the release point as the center, either on the left or on the right. The time was 15 minutes, and the number of biological replicates was ≥12.

[0069] Experimental group 1: One side of the acrylic tube was a filter paper with 10 μL paraffin oil solvent added, and the other side was a filter paper with 10 μL ethyl hexanoate (10 -3 mg / μL) filter paper. Figure 1 As shown, 10 housefly larvae were released from the middle opening (2) each time, and the number of larvae on both sides of the acrylic tube was observed. The number of larvae on the left or right was counted with the release point as the center. The timing was 15 minutes. The positions of the solvent and the ester compound were randomly changed between different biological replicates. The number of biological replicates was ≥12.

[0070] Experimental group 2: One side of the acrylic tube was a filter paper with 10 μL paraffin oil solvent added, and the other side was a filter paper with 10 μL methyl thiobutyrate (10 -3 mg / μL) filter paper. Figure 1 As shown, 10 housefly larvae were released from the middle opening (2) each time, and the number of larvae on both sides of the acrylic tube was observed. The number of larvae on the left or right was counted with the release point as the center. The timing was 15 minutes. The positions of the solvent and the ester compound were randomly changed between different biological replicates. The number of biological replicates was ≥12.

[0071] Experimental group 3: One side of the acrylic tube was a filter paper with 10 μL paraffin oil solvent added, and the other side was a combination of two ester compounds with a mass ratio of 1:1, 9:1, 1:9, 7:3, and 3:7 (total mass concentration of 2×10 -3 mg / μL) filter paper. Figure 1 As shown, 10 housefly larvae were released from the middle opening (2) each time, and the number of larvae on both sides of the acrylic tube was observed. The number of larvae on the left or right was counted with the release point as the center. The timing was 15 minutes. The positions of the solvent and the ester compound were randomly changed between different biological replicates. The number of biological replicates was ≥12.

[0072] Calculate the preference index = (number of insects on the ester compound side - number of insects on the control side) / total number of insects. One-way ANOVA LSD test was used for significant difference analysis and graphing. The results are shown in Figure 2 As shown in Table 1, the same letters indicate that p>0.05, i.e., there is no significant difference between the different tested ester compounds; different lowercase letters indicate that p<0.05, i.e., there is a significant difference between the different compounds.

[0073] Table 1 The preference index of housefly larvae for different ester compounds

[0074] Esters Preference index (mean ± standard error) Paraffin oil (solvent control) <![CDATA[0.03±0.08 c <!-- 5 -->]]> Methyl thiobutyrate <![CDATA[0.45±0.11 ab ]]> Ethyl hexanoate <![CDATA[0.36±0.10 b ]]> 1:1 Composition <![CDATA[0.66±0.08 a ]]> 9:1 ​​Composition <![CDATA[0.48±0.11 ab ]]> 1:9 composition <![CDATA[0.51±0.09 ab ]]> 7:3 Composition <![CDATA[0.62±0.10 a ]]> 3:7 Composition <![CDATA[0.57±0.09 ab ]]>

[0075] from Figure 2 As shown in Table 1, 10 -3 When ethyl hexanoate or methyl thiobutyrate was used as an attractant at a concentration of 100 mg / μL, the preference index of housefly larvae was significantly different from that of the control paraffin oil. The preference index of the binary combination of methyl thiobutyrate and ethyl hexanoate was higher than that of the two ester compounds alone. The higher the preference index, the better the attractant effect, indicating that the binary combination of the two ester compounds has a certain synergistic effect.

[0076] The above results show that methyl thiobutyrate, ethyl hexanoate and the combination of the two ester compounds in a mass ratio of 1:1, 9:1, 1:9, 7:3, and 3:7 all have an attracting effect on housefly larvae, and the combination of the two ester compounds in a mass ratio of 1:1 and 3:7 has a better attracting effect, with a synergistic effect. On this basis, behavior regulators such as attractants or food attractants can be developed to provide theoretical guidance and basis for the green control of houseflies and the factory production of fly maggots.

[0077] Application Example 2

[0078] Experiment on two-way selection trapping and killing of housefly larvae

[0079] 1. Preparation of housefly larvae trap

[0080] An acrylic tube body (5) with a total length of 9 cm and an inner diameter of 2.3 cm was customized; there was a circular opening with a diameter of 1 cm at the center of the top for releasing larvae (7); there were circular openings with a diameter of 1 cm at the left and right sides 1 cm away from the ends as insecticidal traps (9) (a cylindrical trap with an inner diameter of 1 cm was glued with a hot melt glue gun for adding insecticidal reagents); during the experiment, a piece of agarose gel with a diameter of 2.3 cm and a height of 1 cm was placed on each side of the acrylic tube as a sealing cover (6) to block both sides of the tube opening; a square filter paper sheet with a side length of 1 cm (8) was glued to the inside of the gel for adding attractants.

[0081] Pour 1% agarose solution (Agarose, full gold) into a cylinder with an inner diameter of 2.3 cm and a height of 1 cm to shape it, and obtain a cylindrical agarose gel block sealing cap (6);

[0082] The obtained cylindrical agarose gel block is inserted into both sides of the custom acrylic tube (5) to close the openings on both sides of the tube, and the obtained Figure 3 The housefly larvae trap shown.

[0083] 2. Preparation of agents with insecticidal effects

[0084] Using dimethyl sulfoxide as solvent, weigh 10 mg of bifenthrin original drug, dilute to a total volume of 1000 μL, vortex and shake to mix, and obtain a bifenthrin solution with a concentration of 10000 mg / L. Then measure 100 μL of the 10000 mg / L bifenthrin solution, dilute to a total volume of 10 mL, vortex and shake to mix, and obtain an insecticidal reagent containing bifenthrin at a concentration of 100 mg / L.

[0085] Using dimethyl sulfoxide as solvent, weigh 10 mg of trifluoxetine original drug, dilute to a total volume of 1000 μL, vortex and shake to mix, and obtain a trifluoxetine solution with a concentration of 10000 mg / L. Then, measure 100 μL of the 10000 mg / L trifluoxetine solution, dilute to a total volume of 10 mL, vortex and shake to mix, and obtain an insecticidal reagent containing trifluoxetine at a concentration of 100 mg / L.

[0086] 3. Preparation of ester compound carrier

[0087] Clean filter paper is cut into several square filter paper pieces (8) with a side length of 1 cm and used as carriers for loading ester compounds.

[0088] 4. Pre-starvation treatment of housefly larvae for experiments

[0089] Housefly larvae at the end of the second instar or the beginning of the third instar were picked out from the conventional larval culture medium, cleaned, transferred to a culture dish (9 cm in diameter, with a lid) containing 1% agarose gel and allowed to move freely. They were starved for 2 hours before use in the experiment.

[0090] 5. Experiment on two-way selection trapping of housefly larvae

[0091] The experiment was conducted in the insect breeding room 319 of the Nankai University Student Testing Building with a temperature of 26±1°C, a relative humidity of 50±3%, and a photoperiod of 14 h light / 10 h dark.

[0092] Control group: Use a pipette to draw 400 μL of bifenthrin or triflumuron (100 mg / L) and add it to the insect traps on both sides of the housefly larvae trap (9). Place a cut filter paper (8) on the shaped agarose gel block, use a pipette to draw 20 μL of paraffin oil solvent and drop it on the filter paper, and then insert the agarose gel block with the filter paper back into the two sides of the acrylic tube. Figure 3 As shown, 30 housefly larvae were released from the middle opening (7) each time, and the total number of larvae on both sides of the acrylic tube and the number of larvae that died at the place where the insecticide was added (in the trap) were observed and recorded. The time was 30 minutes, and the number of biological replicates was ≥12.

[0093] Experimental group 1: 400 μL of bifenthrin or triflumuron (100 mg / L) was added to the insecticidal traps (9) on both sides of the housefly larvae trap. A cut piece of filter paper (8) was placed on the shaped agarose gel block. 20 μL of paraffin oil solvent and ethyl hexanoate (10 -3 mg / μL) were dripped onto the filter paper sheets (8) on both sides, and then the agarose gel sealing caps (6) with the filter paper sheets were inserted back into the two sides of the acrylic tube. Figure 3 As shown, 30 housefly larvae were released from the middle opening (7) each time, and the total number of larvae on both sides of the acrylic tube and the number of larvae that died in the insecticidal trap (9) were observed and recorded. The timing was 30 minutes, and the number of biological replicates was ≥12.

[0094] Experimental group 2: 400 μL of bifenthrin or triflumuron (100 mg / L) was pipetted into the insecticidal traps (9) on both sides of the housefly larvae trap. A cut piece of filter paper was placed on the shaped agarose gel block. 20 μL of paraffin oil solvent and methyl thiobutyrate (10 -3 mg / μL) were dripped onto the filter paper sheets (8) on both sides, and then the agarose gel sealing caps (6) with the filter paper sheets were inserted back into the two sides of the acrylic tube. Figure 3As shown, 30 housefly larvae were released from the middle opening (7) each time, and the total number of larvae on both sides of the acrylic tube and the number of larvae that died in the insecticidal trap (9) were observed and recorded. The timing was 30 minutes, and the number of biological replicates was ≥12.

[0095] Experimental group 3: 400 μL of bifenthrin or triflumuron (100 mg / L) was pipetted into the insecticidal traps (9) on both sides of the housefly larvae trapping device, a cut piece of filter paper (8) was placed on the shaped agarose gel block, and 20 μL of a combination of paraffin oil solvent and two ester compounds (methyl thiobutyrate and ethyl hexanoate) in a mass ratio of 1:1 (total mass concentration of 2×10 -3 mg / μL) were dripped onto the filter paper sheets (8) on both sides, and then the agarose gel sealing caps (6) with the filter paper sheets were inserted back into the two sides of the acrylic tube. Figure 3 As shown, 30 housefly larvae were released from the middle opening (7) each time, and the total number of larvae on both sides of the acrylic tube and the number of larvae that died in the insecticidal trap (9) were observed and recorded. The timing was 30 minutes, and the number of biological replicates was ≥12.

[0096] Calculate the preference index and trapping index. Preference index = (total number of insects on the ester compound side - total number of insects on the control side) / total number of insects. Trapping index = (number of dead insects in traps on the ester compound side - number of dead insects in traps on the control side) / (number of dead insects in traps on the ester compound side + number of dead insects in traps on the control side). One-way ANOVA LSD test was used for significant difference analysis and graphing. The results are shown in Figure 4 As shown in Table 2, the same letters indicate that p>0.05, i.e., there is no significant difference among the different tested ester compounds; different lowercase letters indicate that p<0.05, i.e., there is a significant difference among the different tested ester compounds.

[0097] Table 2 The preference index and trapping index of housefly larvae of different ester compounds

[0098]

[0099] Depend on Figure 4 From A and C in Table 2, we can see that 10 -3 When methyl thiobutyrate or ethyl hexanoate were present alone or as a binary combination at a concentration of 100 mg / μL as attractants, the preference index of housefly larvae was significantly different from that of paraffin oil solvent. Figure 4 From B and D in Table 2, we can see that 10 -3 When methyl thiobutyrate and ethyl hexanoate were present alone or as a binary combination at a concentration of 100 mg / μL as attractants, and combined with cypermethrin or triflumuron, the killing index of housefly larvae was significantly different from that of paraffin oil solvent.

[0100] The above results show that methyl thiobutyrate, ethyl hexanoate or a combination of the two ester compounds in a mass ratio of 1:1 all have an attractant effect on housefly larvae. When methyl thiobutyrate, ethyl hexanoate or a combination of the two ester compounds in a mass ratio of 1:1 is used as an attractant, combined with bifenthrin or triflumuron, it has a trapping and killing effect on housefly larvae. On this basis, attractants or trapping devices can be developed to provide theoretical guidance and basis for the green control of houseflies and the factory production of fly maggots.

[0101] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creative work, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A housefly larvae attractant, characterized in that: The invention comprises an active ingredient and a solvent, wherein the active ingredient comprises methyl thiobutyrate and / or ethyl n-hexanoate.

2. The housefly larvae attractant according to claim 1, characterized in that: The solvent includes paraffin oil.

3. The housefly larvae attractant according to claim 1, characterized in that: The mass concentration of the active ingredient in the housefly larvae attractant includes 10 -3 mg / μL~10 -2 mg / μL.

4. A method for preparing the housefly larvae attractant according to any one of claims 1 to 3, comprising the following steps: The active ingredient and the solvent are mixed to obtain a housefly larvae attractant.

5. Use of the housefly larvae attractant according to any one of claims 1 to 3 or the housefly larvae attractant prepared by the preparation method according to claim 4 in concentrated trapping and killing of housefly larvae and / or in luring housefly larvae.

6. A product containing the housefly larvae attractant, characterized in that: The product comprises a housefly larvae attractant and auxiliary materials, wherein the auxiliary materials comprise feed.

7. A housefly larvae attractant, characterized in that: The trapping agent includes an attractant and an agent having an insecticidal effect; The attractant includes the housefly larvae attractant according to any one of claims 1 to 3 or the housefly larvae attractant prepared by the preparation method according to claim 4.

8. The housefly larvae attractant according to claim 7, characterized in that: The active ingredients of the insecticide include bifenthrin and / or triflumuron.

9. A housefly larvae trapping and killing device, characterized in that: The housefly larvae trapping device comprises a tube body (5) and a sealing cover (6) arranged at the tube mouth of the tube body; the tube body is provided with an opening (7) for releasing the larvae; the sealing cover is made of agarose gel, and a filter paper (8) for adding an attractant is arranged inside the sealing cover; and an insecticidal trap (9) for adding an insecticidal agent is arranged at a position of the tube body close to the sealing cover.

10. Use of the housefly larvae attractant according to any one of claims 1 to 3 or the housefly larvae attractant prepared by the preparation method according to claim 4 and / or the housefly larvae trapping device according to claim 9 in pest control.

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