Application of dihydroquercetin as wheat seed initiator and initiation method

Abstract

The invention provides application of dihydroquercetin as a wheat seed initiator and an initiation method, and belongs to the technical field of crop cultivation. The initiation method provided by the invention comprises the following steps: preparing 0.1-1.0 mmol.L <-1 > of a dihydroquercetin solution initiator, and mixing seeds and the initiator according to a ratio of 1g: (0.5-1.5) mL for initiation. Dihydroquercetin can effectively prevent and control absorption and accumulation of heavy metal cadmium by wheat and reduce the content of the heavy metal cadmium in grains, so that the content of the cadmium in the grains meets the standard (GB 2762-2022, the grain Cd limit value is 0.1 mg.kg <-1 >) of National Food Safety Standard Limit of Pollutants in Pollutants in Food.

Description

Technical Field

[0001] The invention belongs to the technical field of crop cultivation, and particularly relates to the application of dihydroquercetin as a wheat seed initiator and an initiation method. Background Art

[0002] Cadmium (Cd) is a heavy metal with high biotoxicity among farmland pollutants. It has strong biomobility, non-degradability, high toxicity, and enrichment. It is easily absorbed by plants and enters the food chain, affecting human and animal health. The concentration of Cd in food varies, but plant foods such as cereals and vegetables are usually the largest source of chronic Cd intake for humans. Wheat is one of the most important food crops in my country and has a strong ability to accumulate Cd, which increases human health risks through the food chain. Therefore, in order to ensure food safety and control health risks, effective measures need to be taken to reduce the accumulation of Cd in wheat.

[0003] The existing control technologies are mainly passivation and foliar control. At present, there are no reports in China on seed-induced technology to control wheat Cd accumulation. For example, patent CN109971482A provides a composite conditioner suitable for Cd contamination of winter wheat in alkaline soil and its preparation method, which includes 2-6 parts of manganese oxide, 15-30 parts of zinc sulfate, 2-4 parts of magnesium sulfate, 2-4 parts of iron oxide, 3-6 parts of boric acid and 100-200 parts of apatite by weight; the conditioner can improve the soil, passivate the soil heavy metal Cd and inhibit wheat Cd absorption. Although soil passivators can effectively control the migration of Cd from soil to crops in the short term, as the soil passivators age, the Cd that was passivated earlier will be released again, which will once again pose a threat to food safety and human health. For example, patent CN115872815A discloses a heavy metal foliar barrier for wheat, which specifically includes 20-50g / L selenium salt, 15-35g / L silicon fertilizer, 10-20g / L potassium salt, 1.5-4g / L zinc salt; it also includes: 10-15g / L r-polyglutamic acid, 20-50g / L chitosan oligosaccharide, 20-40g / L Panax notoginseng total saponin and 25-35g / L salicylic acid; the foliar barrier can significantly reduce the heavy metal content in wheat grains, and the Cd content is reduced by 28-45%. However, according to the description of the preparation method of the foliar barrier in the patent, the formula of the solution is relatively complex and the raw material cost is high, which is not conducive to its promotion and use in actual production. Summary of the invention

[0004] In view of this, the purpose of the present invention is to provide an application of dihydroquercetin as a wheat seed initiator and an initiation method, which can prevent and control the accumulation of heavy metal cadmium in wheat and reduce the cadmium content in grains, and the raw materials are sufficient, the price is low, and the operation is simple.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] Application of dihydroquercetin as a wheat seed priming agent.

[0007] Preferably, the effective concentration of dihydroquercetin is 0.1-1.0 mmol·L -1 .

[0008] Preferably, the dihydroquercetin blocks the accumulation of heavy metal cadmium in wheat and reduces the cadmium content in grains.

[0009] The present invention also provides a wheat seed priming method, comprising the following steps: preparing 0.1-1.0 mmol·L -1 The dihydroquercetin solution initiator is mixed at a ratio of 1g of seeds to 0.5-1.5mL for initiation.

[0010] Preferably, the wheat seeds are disinfected before initiation, disinfected with 1% sodium hypochlorite solution for 5-15 minutes, washed with clean water, and dried for later use.

[0011] Preferably, the initiation is performed under light-protected conditions.

[0012] Preferably, the initiating day and night temperature is 26-30°C / 24-26°C, and the humidity is 70%-75%.

[0013] Preferably, the initiation time is ≥16 h, until the wheat seeds are fully imbibed.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The present invention provides an application of dihydroquercetin as a wheat seed initiator and an initiation method thereof. Dihydroquercetin can effectively prevent wheat from absorbing and accumulating heavy metal cadmium, reduce the content of heavy metal cadmium in grains, and make the cadmium content in grains meet the standards of the National Food Safety Standard Limits of Contaminants in Food (GB 2762-2022, Cereal Cd Limit 0.1 mg kg -1 ); and the raw materials of dihydroquercetin are green, environmentally friendly, abundant and cheap. The seed-induced method is convenient to operate, economical and effective, and is not likely to cause secondary pollution to the soil environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The effect of different concentrations of dihydroquercetin solution on Cd content in wheat grains. DETAILED DESCRIPTION

[0017] The invention provides application of dihydroquercetin as a wheat seed initiator.

[0018] In the present invention, the effective concentration of dihydroquercetin is preferably 0.1-1.0 mmol·L -1 , more preferably 0.4-0.9mmol·L -1 , more preferably 0.8 mmol·L -1 Dihydroquercetin can prevent the accumulation of heavy metal cadmium in wheat, reduce the cadmium content in grains, and make the cadmium content in grains meet the standards of the National Food Safety Standard for Limits of Contaminants in Food (GB 2762-2022, Cereal Cd Limit 0.1 mg kg -1 ).

[0019] The present invention also provides a wheat seed priming method, comprising the following steps: preparing 0.1-1.0 mmol·L -1 The initiator is a dihydroquercetin solution, and the initiator is mixed at a ratio of 1g of seed to 0.5-1.5mL for initiation. It is further preferred that the concentration of the dihydroquercetin solution is 0.4-0.9mmol·L -1 , more preferably 0.8 mmol·L -1 ; It is further preferred that the mixing ratio of seeds to initiators is 1g:0.8-1.2mL, and more preferably 1g:1mL. As an implementation method, a culture dish is selected, two layers of filter paper are laid on the bottom, seeds are laid, and evenly placed on the filter paper, and then a layer of plastic wrap is covered on the top of the culture dish, and an appropriate amount of initiator is added to the culture medium for initiation.

[0020] In the present invention, the wheat seeds are preferably disinfected before initiation, disinfected with 1% sodium hypochlorite solution for 5-15 minutes, washed with clean water, and dried for later use; more preferably, the disinfection is performed for 10 minutes. As an implementation method, the moisture is absorbed with absorbent paper (filter paper) for later use.

[0021] In the present invention, the initiation is preferably carried out under light-proof conditions; the preferred day and night temperature for initiation is 26-30°C / 24-26°C, more preferably 28 / 25°C; the preferred initiation humidity is 70%-75%, more preferably 72-73%. As an embodiment, seed initiation is carried out in an incubator.

[0022] In the present invention, the initiation time is preferably ≥ 16 h, until the wheat seeds are fully imbibed.

[0023] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0024] Example 1

[0025] A wheat seed priming method, comprising the following steps:

[0026] Configuration 0.8mmol·L -1The dihydroquercetin solution was used as the wheat seed initiator; uniform and plump wheat seeds were selected, disinfected with 1% sodium hypochlorite solution for 10 minutes, washed repeatedly with deionized water, and the moisture was absorbed with filter paper for later use; the wheat seeds were mixed with the dihydroquercetin solution in a ratio of 1g:1mL for initiation, placed in an incubator, and the day and night temperature was set to 28℃ / 25℃ and the humidity was 73%; initiation was performed for ≥16h until the wheat seeds were fully imbibed and the initiation was completed.

[0027] Example 2

[0028] A wheat seed priming method, comprising the following steps:

[0029] Configuration 0.1mmol·L -1 The dihydroquercetin solution was used as the wheat seed initiator; uniform and plump wheat seeds were selected, disinfected with 1% sodium hypochlorite solution for 5 minutes, washed repeatedly with deionized water, and the moisture was absorbed with filter paper for later use; the wheat seeds were mixed with the dihydroquercetin solution in a ratio of 1g:1mL for initiation, placed in an incubator, and the day and night temperature was set to 26℃ / 24℃ and the humidity was 75%; initiation was performed for ≥16h until the wheat seeds were fully imbibed and the initiation was completed.

[0030] Example 3

[0031] A wheat seed priming method, comprising the following steps:

[0032] Configuration 1.0mmol·L -1 The dihydroquercetin solution was used as the wheat seed initiator; uniform and plump wheat seeds were selected, disinfected with 1% sodium hypochlorite solution for 15 minutes, washed repeatedly with deionized water, and the moisture was absorbed with filter paper for later use; the wheat seeds were mixed with the dihydroquercetin solution in a ratio of 1g:1mL for initiation, placed in an incubator, and the day and night temperature was set to 30℃ / 26℃ and the humidity was 70%; initiation was performed for ≥16h until the wheat seeds were fully imbibed and the initiation was completed.

[0033] Example 4

[0034] 1. Initiator preparation

[0035] The molar mass of dihydroquercetin (DHQ) is 304.25 g·mol -1 , weigh 0.015g, 0.061g, and 0.122g (the error does not exceed ±0.0002g) and add them into 500mL (25℃) water, stir evenly, and prepare the concentration to 0.1mmol·L -1 , 0.4mmol·L -1 and 0.8mmol·L -1 DHQ initiator.

[0036] 2. Experimental Design

[0037] The field plot experiment was conducted in Aoping Town, Pengzhou City, Sichuan Province. The background value of Cd in the soil was 0.53 mg kg -1 The experiment set up four treatments: CK, T1, T2, and T3, with corresponding initiation concentrations of 0, 0.1, 0.4, and 0.8 mmol·L -1 . Select full and uniform wheat seeds, and sow them in each plot after seed priming treatment (Example 1). Apply 0.75 kg of compound fertilizer (total nutrients ≥ 51%, NP 2 O 5 -K 2 O=17-17-17) was used as base fertilizer and evenly spread in each plot. After wheat matured, it was harvested according to the 5-point sampling method.

[0038] 3. Data Collection

[0039] After the wheat matured, the grain samples of each treatment were harvested and dried to constant weight. Each sample was crushed and sieved using a small grinder, and 1 g was weighed. A mixed acid (8 mL HNO 3 and 2 mL HClO 4 ) for 12 h and placed on a graphene hot plate at 220°C until the sample was digested and clarified. After cooling to room temperature, 2% HClO 4 The digestion solution was diluted to 25 mL and then filtered (0.45 μm) into a clean 10 mL centrifuge tube. The Cd content in the filtrate was determined using an inductively coupled plasma mass spectrometer (ICP-MS).

[0040] 4. Test results

[0041] Effects of different concentrations of DHQ seed priming on Cd content in wheat grains are shown in Figure 1 . Compared with CK, DHQ seed priming treatment can significantly reduce the Cd content in wheat grains, and with the increase of treatment concentration, the Cd content in the grains showed a trend of gradual decrease. The Cd content of grains under T1, T2, and T3 treatments decreased by 51.45%, 74.25%, and 95.41%, respectively, which was significantly different from CK (P<0.05). In addition, when the DHQ priming concentration was ≥T2, the Cd content in the grains met the standards of the National Food Safety Standard Limits of Contaminants in Food (GB 2762-2022, Cereal Cd Limit 0.1 mg kg -1 ).

[0042] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Application of dihydroquercetin as a wheat seed initiator.

2. The use according to claim 1, characterized in that: The effective concentration of dihydroquercetin is 0.1-1.0 mmol·L -1 .

3. The use according to claim 1, characterized in that: The dihydroquercetin blocks the accumulation of heavy metal cadmium in wheat and reduces the cadmium content in grains.

4. A wheat seed priming method, characterized in that: The following steps are involved: Configuration 0.1-1.0mmol·L -1 The dihydroquercetin solution initiator is mixed at a ratio of 1g of seeds to 0.5-1.5mL for initiation.

5. The initiation method according to claim 4, characterized in that: The wheat seeds are disinfected before initiation, disinfected with 1% sodium hypochlorite solution for 5-15 minutes, washed with clean water, and air-dried for later use.

6. The initiation method according to claim 4, characterized in that: The initiation was carried out under light-protected conditions.

7. The initiation method according to claim 4, characterized in that: The initiating day and night temperature is 26-30°C / 24-26°C, and the humidity is 70%-75%.

8. The initiation method according to claim 4, characterized in that: The initiation time is ≥16h, until the wheat seeds are fully imbibed.

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