Self-alignment cluster optical fiber probe for parathyroid gland fluorescence detector

Abstract

The invention discloses a self-aligned cluster optical fiber probe for a parathyroid gland fluorescence detector. The self-aligned cluster optical fiber probe specifically comprises a cluster optical fiber probe, an excitation optical fiber, a collection optical fiber, a metal tube, an optical fiber plug group, a locking ring and a connector guide rail base, wherein the metal tube wraps the excitation optical fiber and the collection optical fibers, the collection optical fibers are uniformly arranged on the circumference of the excitation optical fiber and coaxially placed in the metal tube, and one end of the optical fiber is connected with the quick-release self-locking structure wrapping the light plug group. The rapid assembly and disassembly of the bundled optical fiber probe are realized through the rapid disassembly self-locking structure, and the fluorescence collection efficiency is improved by combining the bundled optical fiber.

Description

Technical Field

[0001] The invention relates to the technical field of optical detection, in particular to a self-aligning clustered optical fiber probe for a parathyroid fluorescence detector. Background Art

[0002] Optical fiber has the advantages of light weight, insulation, resistance to electromagnetic interference and anti-anti-radiation interference, and is increasingly used in the field of sensors. During the operation, a fluorescence intensity detector with optical fiber as the probe was developed based on the principle of parathyroid autofluorescence.

[0003] At present, the traditional structure of the parathyroid fluorescence intensity detector probe is two optical fibers, one for transmitting excitation light and the other for collecting fluorescence. The main purpose of this is to shorten the time for replacing the optical fiber probe and reduce the cumbersome operating procedures by limiting the number of optical fibers, for example: ① Parathyroid eye (PTeye) optical fiber probe produced by Medtronic in the United States; ② PTS series fluorescence detector optical fiber probe produced by Yikangda. The disadvantages of this are also very obvious, for example: ① When the optical fiber probe composed of two optical fibers is connected to the host, the locking method is to tighten the connecting ring of each optical fiber, and the two optical fibers cannot be connected to the host in one operation (each connecting ring needs to be twisted 5 times, and it takes 3 to 5 seconds for a single optical fiber to align from the notch to complete the connection); ② The fluorescence collection efficiency of a single collecting optical fiber is low and the sensitivity is low ( Theoretical sensitivity: PT eye ≥ 97%; PTS-200 ≥ 96%). Although the use of bundled optical fibers can solve the problem of fluorescence collection efficiency, if a single optical fiber is still used for fixing, it will bring more problems, such as: ① It takes longer to replace the optical fiber probe; ② The operation is more complicated; ③ The optical fiber probe is larger in size. Summary of the invention

[0004] In order to solve the problem of self-alignment of the optical fiber probe, the present invention designs a self-aligning optical fiber probe. The structure has an embedded optical fiber plug to improve the coupling efficiency between the optical fiber and the host. The quick-release self-locking structure is provided with a dual-guide mechanical structure, which can play a role of quick alignment when used. After alignment, the optical plug group can be completely pressed into the interface of the host by rotating the locking ring half a circle, and the connection and fixation of the optical fiber probe and the host can be realized, and the operation time is short. The self-aligning clustering optical fiber probe for the parathyroid fluorescence detector specifically includes: a clustering optical fiber probe, an excitation optical fiber, a collecting optical fiber, a metal tube, an optical fiber plug group, a locking ring, and a connector guide base; wherein the metal tube wraps the excitation optical fiber and the collecting optical fiber, and the collecting optical fiber is evenly arranged around the circumference of the excitation optical fiber and coaxially placed in the metal tube, and the quick-release self-locking structure wrapping the optical plug group is connected to one end of the optical fiber;

[0005] The locking ring and the connector guide rail base are connected by a mechanical guide rail fixing method to form a quick-release self-locking structure; the optical fiber plug group embedded in the quick-release self-locking structure is pressed against the interface of the host by rotating the locking ring, thereby fixing the optical fiber probe;

[0006] The excitation light emitted by the host is transmitted from the excitation optical fiber of the bundled optical fiber probe to the sample parathyroid gland through the optical fiber plug group, and the fluorescence excited by the sample parathyroid gland is transmitted from the collection optical fiber of the bundled optical fiber probe to the photoelectric sensor of the host through the light plug group.

[0007] The locking ring is provided with a spiral groove at a symmetrical position, wherein the groove width is 2mm to 3mm, the inclination angle of the spiral groove is 30° to 45°, the end of the spiral groove is a semicircular fixing groove, and the radius of the semicircular fixing groove is 1mm to 2mm; on the inner surface of the connector guide rail base, there is a protrusion with a length of 8mm to 10mm, a width of 1mm to 2mm, and a height of 1mm to 2mm, which is aligned with the groove of the host, and the spiral groove of the locking ring and the protrusion of the connector guide rail base form a dual guiding mechanical structure of a quick-release self-locking structure.

[0008] The inner diameter of the connector guide rail base is 28mm~30mm, and the wall thickness is 1mm~2mm; the inner diameter of the locking ring is 30mm~32mm, and the wall thickness is 2mm~3mm, and grooves with a depth of 1mm~2mm are equidistantly engraved on its outer surface to increase friction; the diameter of the quick-release self-locking structure is 34mm~36mm and the length is 40mm~45mm.

[0009] The diameter of the excitation optical fiber is 0.8 mm to 1 mm, and the diameter of the collection optical fiber is 0.6 mm to 0.8 mm.

[0010] The excitation optical fiber is coaxially placed with the metal tube, wherein the length of the excitation optical fiber is 350mm to 400mm; the collection optical fiber is coaxially placed with the metal tube, wherein the length of the collection optical fiber is 350mm to 400mm, and the collection optical fibers are regularly arranged around the excitation optical fiber.

[0011] The self-aligning bundled fiber optic probe is installed in a parathyroid fluorescence detector.

[0012] Due to the adoption of the above-mentioned technical scheme, the present invention provides a self-aligning clustered fiber optic probe for a parathyroid fluorescence detector. The present invention realizes the rapid installation and disassembly of the clustered fiber optic probe through a quick-release self-locking structure, and combines the clustered optical fiber to improve the fluorescence collection efficiency. The quick-release self-locking structure designed by the present invention solves the problem of inconvenience caused by cumbersome operation when connecting the fiber optic probe to the host. In addition, the connector guide base is designed with a dual guide mechanical structure to solve the problem of rapid self-alignment when the fiber optic probe is connected to the host. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0014] Figure 1 It is a schematic structural diagram of a self-aligning clustered optical fiber probe for a parathyroid fluorescence detector of the present invention;

[0015] Figure 2 A schematic diagram of the structure of the connection between the self-aligning optical fiber probe and the host in an embodiment of the present invention;

[0016] Figure 3 This is the signal response spectrum of the detector in Example 1. DETAILED DESCRIPTION

[0017] In order to make the technical solutions and advantages of the present invention more clear, the technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention:

[0018] like Figure 1 The self-aligning clustered fiber optic probe for a parathyroid fluorescence detector shown in the figure comprises a clustered fiber optic probe 1, an excitation fiber 2, a collection fiber optic 3, a metal tube 4, a light plug set 6, a locking ring 8, and a connector guide base 9.

[0019] The locking ring 8 and the connector guide rail base 9 are fixed by mechanical guide rails to form a quick-release self-locking structure 5. A spiral groove is opened at a symmetrical position of the locking ring 8. The groove width is 2mm~3mm, the inclination angle of the spiral groove is 30°~45°, and the end of the spiral groove is a semicircular fixing groove with a radius of 1mm~2mm; a protrusion with a length of 8mm~10mm, a width of 1mm~2mm, and a height of 1mm~2mm is provided on the inner surface of the connector guide rail base 9; the spiral groove of the locking ring 8 and the protrusion of the connector guide rail base 9 form a dual-guide mechanical structure of the quick-release self-locking structure 5.

[0020] Align the raised part of the connector guide base 9 with the groove of the main unit 7, and by rotating the locking ring 8, press the light plug group 6 embedded in the quick-release self-locking structure 5 and the interface of the main unit to fix the fiber optic probe; the excitation light emitted by the main unit 7 is transmitted to the sample through the light plug group 6 by the excitation optical fiber 2 of the bundled optical fiber probe 1, and the fluorescence excited by the sample is transmitted to the photoelectric sensor of the main unit 7 through the light plug group 6 by the collection optical fiber 3 of the bundled optical fiber probe 1.

[0021] The metal tube 4 wraps the excitation optical fiber 2 and the collection optical fiber 3. The collection optical fiber 3 is evenly arranged around the circumference of the excitation optical fiber 2 and coaxially placed in the metal tube 4. A quick-release self-locking structure 5 wrapping a light plug group 6 is connected to one end of the optical fiber.

[0022] Furthermore, the inner diameter of the connector guide rail base 9 is 28mm-30mm, and the wall thickness is 1mm-2mm; the inner diameter of the locking ring 8 is 30mm-32mm, and the wall thickness is 2mm-3mm, and grooves with a depth of 1mm-2mm are equidistantly engraved on the outer surface to increase friction; the diameter of the quick-release self-locking structure 5 is 34mm-36mm, and the length is 40mm-45mm;

[0023] Furthermore, the diameter of the excitation optical fiber 2 is 0.8 mm-1 mm, and the diameter of the collection optical fiber 3 is 0.6 mm-0.8 mm;

[0024] Furthermore, the excitation optical fiber 2 is 350mm-400mm long and is coaxially placed with the metal tube 4; the collection optical fiber 3 is 350mm-400mm long and is coaxially placed with the metal tube 4, and is regularly arranged around the excitation optical fiber 2;

[0025] Further, the self-aligning optical fiber probe is installed on a parathyroid fluorescence detector;

[0026] Furthermore, when the optical plug set 6 is connected to each optical fiber constituting the bundled optical fiber probe 1, a refractive compensation liquid (RI matching fluid, RIMF. Xu J, et al. A glycerol assisted light-emitting diode-induced fluorescence detector for capillary flow systems. Talanta 2008, 75 (4), 885-889) is applied on the surface of the optical fiber;

[0027] Furthermore, the various components of the optical fiber probe are sealed and connected using medical UV glue.

[0028] Example 1

[0029] A self-aligning clustered fiber optic probe, which consists of 6 400mm long collection optical fibers evenly arranged around a 395mm long collection optical fiber. The diameter of the fiber optic probe is 5mm. The tail of the optical fiber is connected to the FC fiber optic plug, which is coaxially placed inside the self-locking quick-release structure. The various parts of the fiber optic probe are sealed and connected by UV glue. Figure 1 The components of a bundled optical fiber are shown.

[0030] The probe of the present invention is connected to the handheld parathyroid fluorescence detector host developed by Dalian Institute of Chemical Physics, Chinese Academy of Sciences, and used. Figure 2 The connection between the fiber optic probe and the detector is demonstrated. The CF790 fluorescent dye is placed in a cuvette with a volume of 3 mL and tested in a dark room. The central wavelength of the excitation light source of the parathyroid fluorescence detector is 785 nm, and the central wavelength of the excitation filter is 785 nm; a lens with a focal length of 5 mm is used as a collimating lens; the reflectivity of the dichroic mirror for light with a wavelength less than 810 nm is greater than 99%; the central wavelength of the emission filter is 825 nm; the light trap can absorb more than 99% of the Rayleigh scattered light, and the photoelectric detection device is a photodiode integrated amplifier circuit component (low-light detector) developed by the Dalian Institute of Chemical Physics, Chinese Academy of Sciences.

[0031] Experimental results:

[0032] Figure 3 The signal response spectrum is shown in the concentration range of CF790 fluorescent dye: 1×10 -8 M-2.5×10 -11 M, detection limit is 3.1×10 -11 M. Under the same experimental conditions, the detection limit of the integrated parathyroid fluorescence detector for CF790 was 2.9×10 -11 M. By comparison, it can be seen that the present invention realizes the self-alignment and detachability of the optical fiber probe and the influence on the sensitivity of the detector can be ignored.

[0033] Example 2

[0034] According to the detector conditions of Example 1, the object to be tested is changed to simulated human tissue (hereinafter referred to as "phantom"), and the phantom configuration process is as follows: ① Weigh 40 mg of agar and place it in a centrifuge tube with a volume of 20 mL, add an appropriate amount of deionized water, and vortex to mix; ② Weigh 25 mg of titanium dioxide (simulating tissue scattering characteristics) and 15 mg of hemoglobin (simulating tissue absorption coefficient) and place them in centrifuge tubes respectively, add an appropriate amount of deionized water, and vortex to mix; ③ Place the agar solution in a water bath, set the water bath temperature to 90°C, and after the agar melts, add CF790 solution, titanium dioxide solution, and hemoglobin solution respectively, and vortex to mix; ④ Pour the solution into a culture dish and wait for solidification; ⑤ Embed the phantom containing the fluorescent agent into the phantom without the fluorescent agent.

[0035] The same fluorescent agent concentration (CF790 concentration is 1×10 -10 M) was placed into a phantom without a fluorescent agent at different depths to test the effect of different depths on the collection of fluorescent signals.

[0036] Experimental results:

[0037] As shown in Table 1, it was measured that when the phantom coverage was within 5 mm in depth, the signal of the phantom containing the fluorescent agent was more than 3 times that of the phantom without the fluorescent agent. Based on this, it can be judged that the phantom containing the fluorescent agent can be distinguished from the phantom without the fluorescent agent (judgment basis: in actual use, the intensity of the parathyroid fluorescence signal is 2 to 11 times that of the signal intensity of other surrounding tissues, Constantine Paras, et al. Near-infrared autofluorescence for the detection of parathyroid glands. Journal of Biomedical Optics 2011, 16 (6), 067012). When the phantom containing the fluorescent agent covers a depth of more than 5 mm, the signal difference is within 3 times, and the two cannot be effectively distinguished.

[0038] Table 1 Relationship between the depth of placement of the phantom with fluorescent agent and the signal

[0039]

[0040]

[0041] Compared with the integrated detector, the effective fluorescence signal value collected by the present invention is basically consistent with that of the integrated detector, and has the advantage that the optical fiber probe can be quickly replaced during use, thereby simplifying the operating procedure.

[0042] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A self-aligning clustered optical fiber probe for parathyroid fluorescence detector, characterized in that include: A bundled optical fiber probe (1), an excitation optical fiber (2), a collection optical fiber (3), a metal tube (4), an optical fiber plug group (6), a locking ring (8), and a connector guide rail base (9); wherein the metal tube (4) encloses the excitation optical fiber (2) and the collection optical fiber (3); the collection optical fiber (3) is evenly arranged around the circumference of the excitation optical fiber (2) and coaxially placed in the metal tube (4); and a quick-release self-locking structure (5) enclosing the optical fiber plug group (6) is connected to one end of the optical fiber; The locking ring (8) and the connector guide rail base (9) are connected by a mechanical guide rail fixing method to form a quick-release self-locking structure (5); the optical fiber plug group (6) embedded in the quick-release self-locking structure (5) is pressed against the interface of the host by rotating the locking ring (8), thereby fixing the optical fiber probe; The excitation light emitted by the host (7) is transmitted to the parathyroid glands through the excitation optical fiber (2) of the bundled optical fiber probe (1) via the optical fiber plug group (6), and the fluorescence excited by the parathyroid glands is transmitted to the photoelectric sensor of the host (7) through the collection optical fiber (3) of the bundled optical fiber probe (1) via the light plug group (6).

2. The self-aligning clustered optical fiber probe for parathyroid fluorescence detector according to claim 1, characterized in that: The locking ring (8) is provided with a spiral groove at a symmetrical position, wherein the groove width is 2 mm to 3 mm, the inclination angle of the spiral groove is 30° to 45°, the end of the spiral groove is a semicircular fixing groove, and the radius of the semicircular fixing groove is 1 mm to 2 mm; on the inner surface of the connector guide rail base (9), there is a protrusion with a length of 8 mm to 10 mm, a width of 1 mm to 2 mm, and a height of 1 mm to 2 mm, the protrusion is aligned with the groove of the host (7), and the spiral groove of the locking ring (8) and the protrusion of the connector guide rail base (9) form a double guiding mechanical structure of the quick-release self-locking structure (5).

3. The self-aligning clustered optical fiber probe for parathyroid fluorescence detector according to claim 1, characterized in that: The inner diameter of the connector guide rail base (9) is 28 mm to 30 mm, and the wall thickness is 1 mm to 2 mm; the inner diameter of the locking ring (8) is 30 mm to 32 mm, and the wall thickness is 2 mm to 3 mm, and grooves with a depth of 1 mm to 2 mm are equidistantly engraved on its outer surface to increase friction; the diameter of the quick-release self-locking structure (5) is 34 mm to 36 mm, and the length is 40 mm to 45 mm.

4. The self-aligning clustered optical fiber probe for parathyroid fluorescence detector according to claim 1, characterized in that: The diameter of the excitation optical fiber (2) is 0.8 mm to 1 mm, and the diameter of the collection optical fiber (3) is 0.6 mm to 0.8 mm.

5. The self-aligning clustered optical fiber probe for parathyroid fluorescence detector according to claim 1, characterized in that: The excitation optical fiber (2) is coaxially arranged with the metal tube (4), wherein the length of the excitation optical fiber (2) is 350 mm to 400 mm; the collection optical fiber (3) is coaxially arranged with the metal tube (4), wherein the length of the collection optical fiber (3) is 350 mm to 400 mm, and the collection optical fiber (3) is regularly arranged around the excitation optical fiber (2).

6. An application of a self-aligning clustered optical fiber probe for a parathyroid fluorescence detector, characterized in that: The self-aligning bundled fiber optic probe is installed in a parathyroid fluorescence detector.

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