Traditional Chinese medicine extract with uric acid reducing function as well as preparation method and application of traditional Chinese medicine extract

Abstract

The invention relates to the technical field of traditional Chinese medicine. The invention provides a traditional Chinese medicine extract with a function of reducing uric acid and a preparation method of the traditional Chinese medicine extract. The traditional Chinese medicine extract comprises the following raw materials: chrysanthemum morifolium ramat, radix angelicae, liquorice, perilla leaves and chia seeds. The invention further provides application of the traditional Chinese medicine extract in preparation of functional food and uric acid reducing medicine with the auxiliary uric acid reducing function. The traditional Chinese medicine extract obtained by carrying out water extraction on the medicinal and edible traditional Chinese medicines by utilizing a heating reflux extraction method contains various functional components and has good uric acid reducing activity; and the preparation method is simple in process and relatively good in safety, and has a certain prospect in the aspects of reducing uric acid and treating hyperuricemia.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine extract having the function of reducing uric acid, a preparation method and application thereof. Background Art

[0002] Hyperuricemia is the biochemical basis for gout and uric acid nephropathy, and is a high-risk factor for coronary heart disease, hyperlipidemia, diabetes and other diseases, and can increase the mortality rate of patients with cardiovascular disease and diabetes. According to statistics, the incidence of gout is 0.06-2.68‰, and there is a trend of increasing year by year and the age of onset is getting younger. Therefore, it is of great significance to control hyperuricemia.

[0003] At present, although Western medicine has definite therapeutic effects in the treatment of hyperuricemia, the recurrence rate after discontinuation of the medicine is high, and it has certain toxicity and adverse reactions. It has great limitations for patients with abnormal renal function, and most patients cannot persist in treatment.

[0004] At present, studies have found that traditional Chinese medicine also has a certain effect on the treatment of hyperuricemia, and the recurrence rate is relatively low. Therefore, it is of great clinical value to obtain a traditional Chinese medicine compound with the effect of increasing efficacy and reducing toxicity, and use the traditional Chinese medicine compound to reduce blood uric acid and treat hyperuricemia. Summary of the invention

[0005] The purpose of the present invention is to provide a Chinese medicine extract with the function of lowering uric acid. The Chinese medicine extract obtained by water extraction using the Chinese medicine with medicinal and edible properties by the heating reflux extraction method contains multiple functional ingredients and has good uric acid lowering activity; the process is simple and the safety is good, and it has certain prospects in lowering uric acid and treating hyperuricemia.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides a traditional Chinese medicine extract with the function of reducing uric acid, comprising the following raw materials: chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds.

[0008] Preferably, the mass ratio of the chrysanthemum, angelica dahurica, licorice, perilla leaves and chia seeds is (0.6-1.3):(0.8-1.2):(0.8-1.2):(0.5-1.5):(0.8-1.1).

[0009] The invention provides a method for preparing the Chinese medicine extract, comprising the following steps: mixing chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds to obtain a mixture, mixing the mixture with water at a mass ratio of 1:(10-35), and then extracting to obtain the Chinese medicine extract.

[0010] Preferably, the extraction time is 1-2 h / time, and the number of extractions is 1-3 times.

[0011] The present invention also provides the use of the Chinese medicine extract in preparing functional food with auxiliary uric acid lowering effect.

[0012] The present invention also provides application of the Chinese medicine extract in preparing a uric acid-lowering drug.

[0013] The present invention also provides application of the Chinese medicinal extract in preparing a drug for treating hyperuricemia.

[0014] The present invention also provides a uric acid reducing product containing the Chinese herbal extract, which comprises the following raw materials in parts by weight: 90-110 parts of the Chinese herbal extract, 1-10 parts of α-linolenic acid and 0.5-2 parts of a flavoring agent.

[0015] Preferably, the flavoring agent comprises sucrose, steviol glycoside or glycyrrhizic acid.

[0016] By adopting the above technical solution, the present invention has the following beneficial effects:

[0017] 1. In the technical solution of the present invention, chrysanthemum, angelica dahurica, licorice, perilla leaves and chia seeds work synergistically to prepare a traditional Chinese medicine extract which has the effect of reducing blood uric acid concentration and relieving gout. The ingredients in the extract have the effect of promoting the excretion of uric acid in the body and accelerating the metabolism of uric acid in the body, thereby stabilizing the blood uric acid level in the body, providing a new idea for the treatment of hyperuricemia.

[0018] 2. The Chinese medicine extract provided by the present invention uses chrysanthemum, angelica dahurica, licorice, perilla leaves, and chia seeds as raw materials. The raw materials are widely available, and the preparation process is simple and easy to obtain. It has the characteristics of low production cost. The raw materials are medicinal and edible materials, and can be widely used in the preparation of food, health products and medicines, and have strong market competitiveness. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The standard curve is drawn with rutin mass concentration as the horizontal axis and absorbance as the vertical axis. DETAILED DESCRIPTION

[0020] The invention provides a traditional Chinese medicine extract with the function of reducing uric acid, comprising the following raw materials: chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds.

[0021] In the present invention, the mass ratio of the chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds is preferably (0.6-1.3):(0.8-1.2):(0.8-1.2):(0.5-1.5):(0.8-1.1), further preferably (0.8-1.1):(0.9-1.1):(0.9-1.1):(0.7-1.3):(0.9-1.05), and further preferably 1:1:1:1:1.

[0022] The present invention also provides a method for preparing the Chinese medicine extract, comprising the following steps: mixing chrysanthemum, angelica dahurica, licorice, perilla leaves and chia seeds to obtain a mixture, mixing the mixture with water at a mass ratio of 1:(10-35), and then extracting to obtain the Chinese medicine extract.

[0023] In the present invention, chrysanthemum, angelica dahurica, liquorice, perilla leaf and chia seeds are mixed to obtain a mixture, and then the mixture is mixed with water for extraction to obtain a Chinese medicine extract. The mass ratio of the mixture to water in the present invention is preferably 1: (10-35), further preferably 1: (15-32), and further preferably 1: 30. The extraction method of the present invention is preferably heated reflux extraction, and the heating temperature is preferably 95-100 ° C, and further preferably 98-100 ° C; the extraction time is preferably 1-2h / time, further preferably 1.2-1.8h / time, and further preferably 1.5h / time; the number of extractions is preferably 1-3 times.

[0024] In the present invention, the Chinese herbal medicine extract is preferably a thick paste-like Chinese herbal medicine extract or a powdered Chinese herbal medicine extract. When the Chinese herbal medicine extract of the present invention is a thick paste-like Chinese herbal medicine extract, it is concentrated after the extraction is completed. The present invention has no special restrictions on the concentration method, and the concentration method well known in the art can be used. When the Chinese herbal medicine extract of the present invention is a powdered Chinese herbal medicine extract, it is concentrated and dried in sequence after the extraction is completed. The present invention has no special restrictions on the concentration and drying methods, and the concentration and drying methods well known in the art can be used.

[0025] The present invention also provides the use of the Chinese medicine extract in preparing functional food with auxiliary uric acid lowering effect.

[0026] The present invention also provides application of the Chinese medicine extract in preparing a uric acid-lowering drug.

[0027] The present invention also provides application of the Chinese medicinal extract in preparing a drug for treating hyperuricemia.

[0028] The present invention also provides a uric acid lowering product comprising the Chinese herbal extract, preferably comprising the following weight portions: 90-110 parts of the raw Chinese herbal extract, 1-10 parts of α-linolenic acid and 0.5-2 parts of the flavoring agent, further preferably comprising 95-105 parts of the Chinese herbal extract, 2-8 parts of α-linolenic acid and 0.8-1.5 parts of the flavoring agent, and even more preferably comprising 100 parts of the Chinese herbal extract, 5 parts of α-linolenic acid and 1 part of the flavoring agent.

[0029] In the present invention, the flavoring agent preferably includes sucrose, steviol glycoside or glycyrrhizic acid.

[0030] In the present invention, the uric acid lowering product is preferably a granule, a compressed candy, an oral liquid or a beverage.

[0031] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0032] Example 1

[0033] A Chinese herbal medicine extract with the function of reducing uric acid, comprising chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds in a mass ratio of 1.3:1.2:0.8:1.5:1.1;

[0034] The preparation method of the Chinese medicine extract comprises the following steps: mixing chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds to obtain a mixture, mixing the mixture with water at a mass ratio of 1:30, then heating and refluxing for extraction, extracting at 98° C. for 3 times, each time for 1.5 hours; and then concentrating to obtain a thick paste-like Chinese medicine extract.

[0035] Example 2

[0036] A Chinese herbal medicine extract with the function of reducing uric acid, comprising chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds in a mass ratio of 0.6:0.8:1.2:0.5:0.8;

[0037] The preparation method of the traditional Chinese medicine extract comprises the following steps: mixing chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds to obtain a mixture, mixing the mixture with water in a mass ratio of 1:10, and then heating and refluxing for extraction, extracting once at 100° C. for 1 hour each time; and then concentrating and drying in sequence to obtain a powdered traditional Chinese medicine extract.

[0038] Example 3

[0039] A Chinese herbal medicine extract with the function of reducing uric acid, comprising chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds in a mass ratio of 0.8:0.9:1.1:0.7:1.1;

[0040] The preparation method of the Chinese medicine extract comprises the following steps: mixing chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds to obtain a mixture, mixing the mixture with water at a mass ratio of 1:35, then heating and refluxing for extraction, extracting twice at 100° C., each time for 2 hours; and then concentrating and drying in sequence to obtain a powdered Chinese medicine extract.

[0041] Example 4

[0042] A granule comprising the Chinese herbal extract described in Example 1, wherein the raw materials are 100 parts of the Chinese herbal extract described in Example 1, 5 parts of α-linolenic acid and 1 part of sucrose.

[0043] Example 5

[0044] A compressed candy comprising the Chinese herbal extract described in Example 1, wherein the raw materials are 90 parts of the Chinese herbal extract described in Example 1, 1 part of α-linolenic acid and 2 parts of sucrose.

[0045] Example 6

[0046] An oral liquid comprising the Chinese herbal extract described in Example 1, wherein the raw materials are 110 parts of the Chinese herbal extract described in Example 2, 10 parts of α-linolenic acid and 0.5 parts of sucrose.

[0047] Example 7

[0048] A granule comprising the Chinese herbal extract described in Example 1, wherein the raw materials are 100 parts of the Chinese herbal extract described in Example 2, 5 parts of α-linolenic acid and 1 part of steviol glycoside.

[0049] Example 8

[0050] A granule comprising the Chinese herbal extract described in Example 1, wherein the raw materials are 100 parts of the Chinese herbal extract described in Example 3, 5 parts of α-linolenic acid and 1 part of glycyrrhizic acid.

[0051] Test Example 1 Preparation of uric acid-lowering Chinese herbal extract

[0052] Take 20g of chrysanthemum, 20g of angelica, 20g of licorice, 20g of perilla leaves, and 20g of chia seeds, put them into a 3L round-bottom flask, add A times the mass volume of purified water, heat until boiling, start timing C hours, filter the medicinal solution through a 200-mesh sieve, collect the filtrate, continue to add A times the amount of water for boiling extraction, boil and extract B times, combine the filtrate, concentrate to 500mL, take 50mL, place it in a water bath and concentrate it to a paste, transfer it to a vacuum drying oven and dry it for 24h (the temperature of the vacuum drying oven is set to 70℃), and obtain a dry paste of traditional Chinese medicine extract. After crushing, obtain powder, and calculate the paste rate.

[0053] Extraction rate (%) = (extract powder mass / total mass of decoction pieces) × 100%

[0054] Table 1 Orthogonal experiment table of extraction process

[0055]

[0056] As can be seen from Table 1, the solid-liquid ratio, the number of extractions and the extraction time can all affect the yield of the extract. Compared with the extraction time, the number of extractions has a greater impact on the yield, and the larger the solid-liquid ratio and the number of extractions, the higher the yield. When the solid-liquid ratio is 10-30, the number of extractions is 3 times, and the extraction time is 1.5-2h, the yield can reach about 40%; among them, the highest yield is 41.8% when the solid-liquid ratio is 30, the number of extractions is 3 times, and the extraction time is 1.5h. Considering the actual production needs, the suboptimal process is selected with a solid-liquid ratio of 30, a number of extractions of 2 times, and an extraction time of 1.5h.

[0057] Test Example 2 Determination of total flavonoids content in uric acid-lowering Chinese herbal extracts

[0058] (I) Preparation of reference solution

[0059] Accurately weigh 5.0 mg of rutin reference substance, place it in a 10 mL volumetric flask, add 75% ethanol and sonicate for 30 minutes to dissolve. After cooling, add 75% ethanol to the volume and shake the mixture to prepare a 500 μg / mL reference solution.

[0060] (II) Preparation of test solution

[0061] Accurately weigh 0.5 g of the dry paste samples of the extracts obtained by the 9 orthogonal extraction processes and put them into a 10 mL centrifuge tube, add 9 mL of 70% ethanol, ultrasonicate in a 60°C water bath for 30 min, and use an ultrasonic power of 300 W. Centrifuge the extract at 4000 rpm for 10 min. Transfer the supernatant to a 25 mL volumetric flask. Perform two extractions in total, combine the supernatants, and make up to 25 mL with 70% ethanol.

[0062] (III) Drawing of standard curve

[0063] Get 10mL volumetric flask, accurately pipette 0.25,0.50,0.75,1.0,1.25,1.50mL of rutin reference substance solution respectively, add above-mentioned volumetric flask respectively, then add 50mg / mL successively to obtain sodium nitrite solution 0.3mL, 100mg / mL aluminum nitrate solution 0.3mL, 100mg / mL sodium hydroxide solution 4mL, all need to shake to make mixing after adding above-mentioned solution at each time, add next solution again after standing for 5min, finally use 70% ethanol solution to be settled to 10mL. With rutin blank as contrast, measure its absorbance at 510nm wavelength. With rutin mass concentration (mg / mL) abscissa and absorbance as ordinate drawing standard curve.

[0064] (IV) Determination of total flavonoid content

[0065] Accurately measure 1.0mL of the sample test solution, add it to a 10mL volumetric flask, and add 0.3mL of 50mg / mL sodium nitrite solution, 0.3mL of 100mg / mL aluminum nitrate solution, and 4mL of 100mg / mL sodium hydroxide solution in sequence. Shake well after adding each of the above solutions, and finally make up to 10mL with 70% ethanol solution. Measure the absorbance value on a multifunctional microplate reader and draw a standard curve ( Figure 1 ), and the total flavonoids content of each sample was calculated by regression equation (Table 2).

[0066] Table 2 Total flavonoid content in extracts from different extraction processes

[0067]

[0068]

[0069] It can be seen from Table 2 that the total flavonoids content in the extract is the highest, reaching 3.03 mg / g, when the solid-liquid ratio of the extraction process is 30, the number of extractions is 3 times, and the extraction time is 1.5 h.

[0070] Test Example 3 Determination of glycyrrhizic acid content in uric acid-lowering Chinese herbal extracts

[0071] (I) Chromatographic conditions and system suitability test

[0072] Octadecylsilane bonded silica gel is used as filler; acetonitrile is used as mobile phase A, 0.05% phosphoric acid solution is used as mobile phase B, and gradient elution is performed according to the provisions in the following table; the detection wavelength is 237nm. The theoretical plate number calculated based on the ammonium glycyrrhizinate peak should be no less than 5000.

[0073] Table 3 Elution program

[0074]

[0075] (II) Preparation of reference solution

[0076] Take an appropriate amount of ammonium glycyrrhizinate reference substance, weigh it accurately, and add 70% ethanol to prepare a solution containing 0.2 mg of ammonium glycyrrhizinate per 1 ml.

[0077] Glycyrrhizic acid weight = ammonium glycyrrhizinate weight / 1.0207

[0078] (III) Preparation of test solution

[0079] Take 0.2 g of the powder (passed through No. 3 sieve), weigh accurately, put it in a stoppered conical flask, add 100 ml of 70% ethanol accurately, stopper it, weigh it, treat it with ultrasound (power 250 W, frequency 40 kHz) for 30 minutes, let it cool, weigh it again, make up the lost weight with 70% ethanol, shake it well, filter it, and take the filtrate to obtain the product.

[0080] (IV) Determination

[0081] Accurately pipette 10 μl of the reference solution and the test solution respectively, inject into the liquid chromatograph, measure, and record the chromatogram for 40 minutes.

[0082] Table 4 Glycyrrhizic acid content in extracts from different extraction processes

[0083]

[0084] It can be seen from Table 4 that the glycyrrhizic acid content in the extract is the highest, reaching 0.513 mg / g, when the solid-liquid ratio of the extraction process is 30, the number of extractions is 3 times, and the extraction time is 1.5 h.

[0085] Test Example 4 Evaluation of the efficacy of uric acid-lowering Chinese herbal extracts

[0086] The uric acid lowering dry paste powder obtained in Example 1 was taken, and ten male volunteers were recruited. The dry paste powder was dissolved in warm water at a dose of 8 g / person, and the blood uric acid level of each person was measured on an empty stomach and 1 hour after taking the uric acid lowering Chinese medicine extract. The results are shown in Table 5.

[0087] Table 5 Results of uric acid lowering function test

[0088]

[0089]

[0090] As shown in Table 5, the uric acid levels of 9 people decreased after taking the uric acid-lowering Chinese herbal extract, of which 6 people's blood uric acid levels decreased by more than 5%, 3 people's decreased by more than 10%, 4 people's blood uric acid levels decreased by less than 5%, and 1 person's blood uric acid level did not change. This shows that the uric acid-lowering Chinese herbal extract of the present invention has a significant uric acid-lowering effect.

[0091] From the above, it can be seen that the Chinese medicine extract obtained by water extraction using the heating reflux extraction method through the Chinese medicine with medicinal and edible properties in the present invention contains a variety of functional ingredients and has good uric acid lowering activity; the process is simple and the safety is good, and it has certain prospects in lowering uric acid and treating hyperuricemia.

[0092] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Chinese herbal extract having the function of reducing uric acid, characterized in that: Including the following ingredients: chrysanthemum, angelica root, licorice root, perilla leaf and chia seed; The mass ratio of the chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds is (0.6-1.3):(0.8-1.2):(0.8-1.2):(0.5-1.5):(0.8-1.1).

2. The method for preparing the Chinese herbal extract according to claim 1, characterized in that: The method comprises the following steps: mixing chrysanthemum, angelica dahurica, liquorice, perilla leaves and chia seeds to obtain a mixture, mixing the mixture with water at a mass ratio of 1:(10-35), and then extracting to obtain a traditional Chinese medicine extract.

3. The preparation method according to claim 2, characterized in that: The extraction time is 1-2h / time, and the number of extractions is 1-3 times.

4. Use of the Chinese herbal extract obtained by the preparation method according to claim 2 or 3, or the Chinese herbal extract according to claim 1, in the preparation of functional foods having auxiliary uric acid lowering effects.

5. Use of the Chinese herbal extract obtained by the preparation method according to claim 2 or 3, or the Chinese herbal extract according to claim 1, in the preparation of uric acid-lowering drugs.

6. Use of the Chinese medicinal extract obtained by the preparation method according to claim 2 or 3, or the Chinese medicinal extract according to claim 1, in the preparation of a drug for treating hyperuricemia.

7. A uric acid lowering product comprising the Chinese herbal extract obtained by the preparation method of claim 2 or 3, or the Chinese herbal extract of claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 90-110 parts of Chinese herbal medicine extract, 1-10 parts of alpha-linolenic acid and 0.5-2 parts of flavoring agent.

8. The product according to claim 7, characterized in that The flavoring agent includes sucrose, steviol glycoside or glycyrrhizic acid.

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