Traditional Chinese medicine preparation for treating vertigo and preparation method thereof

Abstract

The invention provides a traditional Chinese medicine preparation for treating vertigo and a preparation method, and belongs to the field of traditional Chinese medicines. The preparation method comprises the following steps: crushing and grinding into powder; performing ultrasonic-assisted enzymolysis extraction to prepare a fluid extract A; performing microwave-assisted heating and pressurizing reflux extraction to prepare a fluid extract B; mixing to prepare the traditional Chinese medicine preparation. The preparation method comprises the following steps: performing ultrasonic-assisted cellulose enzymolysis extraction on radix puerariae and uncaria to prepare a fluid extract A, performing microwave-assisted reflux extraction on angelica sinensis, radix paeoniae alba, selfheal, scutellaria baicalensis, rhizoma anemarrhenae and caulis bambusae in taeniam to prepare a fluid extract B, and mixing the fluid extract A and the fluid extract B with caulis spatholobi, concha margaritifera, ligusticum chuanxiong hort and agastache rugosus which are ground into powder to prepare the traditional Chinese medicine preparation. The Chinese medicinal preparation takes Chinese angelica, suberect spatholobus stem and Sichuan lovage rhizome as monarch drugs, takes nacre mother of pearl, uncaria, white paeony root, selfheal and kudzuvine root as ministerial drugs, takes baical skullcap root and common anemarrhena rhizome as adjuvant drugs, and takes bamboo shavings and wrinkled gianthyssop herb as conductant drugs, so that the Chinese medicinal preparation can play roles in improving insufficient blood supply of heart and brain, improving dizziness and the like, thereby effectively treating vertigo.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicines, and in particular to a traditional Chinese medicine preparation for treating vertigo and a preparation method thereof. Background Art

[0002] Dizziness and vertigo are common symptoms in neurology. Vertigo is a movement or positional illusion that causes a distorted response in the cerebral cortex in the spatial relationship between a person and the surrounding environment, producing illusions such as rotation, tipping, and ups and downs. It is a common disease in neurology, otolaryngology, psychosomatic medicine, and geriatrics. The three most common types of vertigo symptoms can be divided into: phlegm-dampness blocking the middle type, liver yang hyperactivity type, and qi and blood deficiency type. Surveys show that the prevalence of vertigo in the general population aged 18-79 is 4.9%. The main methods for treating vertigo are drug therapy, surgical treatment, and dietary adjustment.

[0003] At present, drug treatment is mainly based on chemical drugs. Chinese herbal preparations approved in the market for the treatment of vertigo are scarce and far from meeting the medication needs of the sick population. Although chemical drugs are quick to take effect in clinical use, they are of a single variety and a small range of choices. Symptoms recur after discontinuation of the drug. If patients take them for a long time, they are prone to drug resistance and adverse reactions, and their safety is not high.

[0004] Therefore, it is necessary to provide a safe and effective Chinese medicine preparation for treating vertigo and a preparation method thereof. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention aims to provide a Chinese medicine preparation for treating vertigo and a preparation method thereof.

[0006] A method for preparing a Chinese medicine preparation for treating vertigo comprises the following steps: S1: Crushing, grinding and powder making Place Millettia spatholobi, mother-of-pearl, Chuanxiong herb and Patchouli in a grinding and milling machine, grind and grind them, and then pass them through an 80-mesh sieve to obtain Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong herb powder and Patchouli powder; S2: Preparation of fluid extract A by ultrasound-assisted enzymatic extraction Methanol, ethanol and deionized water are prepared into a mixed solution for later use, and Pueraria root and Uncaria rhynchophylla are crushed respectively and added into the mixed solution for uniform dispersion, and then cellulase is added, and heated for enzymolysis to inactivate the enzyme, and then ultrasonic treatment is performed, and the fluid extract A is obtained by suction filtration and concentration; S3: Preparation of fluid extract B by microwave-assisted heating and pressurized reflux extraction The Chinese angelica, white peony root, Prunella vulgaris, scutellaria baicalensis, Rhizoma Anemarrhenae and Rhizoma Anemarrhenae were ground and mixed with deionized water, and then subjected to microwave-assisted heating and pressurized reflux extraction, and filtered and concentrated to obtain fluid extract B; S4: Mixing and preparation of traditional Chinese medicine preparations The above-mentioned Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder are uniformly mixed with the above-mentioned fluid extract A and fluid extract B, and then freeze-dried, pulverized, ground, sieved, filled and packaged to obtain a traditional Chinese medicine preparation.

[0007] Furthermore, S2 specifically includes the following steps: S2.1: Methanol, ethanol and deionized water are stirred and mixed uniformly in a volume ratio of 1:(2-4):(5-7) to obtain a mixed solution; S2.2: Grind Puerariae Radix and Uncaria Rhynchophylla Rhizome separately, add them into the above mixed solution at a solid-liquid ratio of 1g:(30-40)mL, and disperse them by ultrasonic for 10-20min to obtain a mixed suspension A; S2.3: Add cellulase to the mixed suspension A, heat at 45-55°C for 1-2h while stirring to perform enzymolysis, and then inactivate the enzyme for 3-5min to obtain an enzymolysis solution; S2.4: Heat the above enzymatic hydrolysate to 60-70°C and perform ultrasonic treatment for 30-40 minutes. After vacuum filtration, place the filtrate in a concentrator for concentration until the volume of the filtrate is concentrated to 1 / 10 of the original volume to obtain fluid extract A.

[0008] Furthermore, S3 specifically includes the following steps: S3.1: Grind angelica sinensis, white peony root, selfheal, scutellaria baicalensis, anemarrhena asphodeloides and bamboo shavings respectively to obtain angelica sinensis powder, white peony root powder, selfheal powder, scutellaria baicalensis powder, anemarrhena asphodeloides powder and bamboo shavings powder; S3.2: Add the above-mentioned angelica powder, white peony powder, selfheal powder, scutellaria powder, anemarrhena powder and bamboo shavings powder into a microwave extractor, and then add deionized water at a material-liquid ratio of 1g: (20-30)mL, stir and mix evenly to obtain a mixed suspension B; S3.3: pressurizing the microwave extractor, heating the mixed suspension B at 100-120°C for 20-30 minutes, subjecting it to microwave treatment, and then continuing to heat for 1-2 hours, subjecting it to microwave-assisted heating and pressurized reflux extraction, and naturally cooling it to room temperature to obtain a mixed extract; S3.4: filtering the mixed extract, and subjecting the residue to microwave-assisted heating and pressurized reflux extraction again, filtering, combining the filtrates, and obtaining a mixed extract; S3.5: Place the mixed extract in a concentrator and heat and concentrate until the volume of the mixed extract is concentrated to 1 / 10 of the original volume, thereby obtaining fluid extract B.

[0009] Furthermore, S4 specifically includes the following steps: S4.1: Add the Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder prepared in step S1, the fluid extract A prepared in step S2.4 and the fluid extract B prepared in step S3.5 into a mixer, mix them thoroughly and evenly to obtain a mixed wet material; S4.2: placing the above mixed wet material in a freeze dryer for freeze drying, and then placing it in a crushing and grinding machine for crushing and grinding, and passing through an 80-mesh sieve to obtain a mixed powder; S4.3: Fill the above mixed powder into hard capsules according to the required amount, and then package them to obtain a Chinese medicine preparation.

[0010] Furthermore, the added amount of cellulase is 0.4-0.6wt%.

[0011] Furthermore, the enzyme inactivation temperature is 90-95°C.

[0012] Furthermore, the pressure of microwave-assisted heating and pressurized extraction is 1-3 MPa.

[0013] Furthermore, the microwave treatment power is 200-300W and the treatment time is 20-30min.

[0014] Furthermore, the Chinese medicine preparation consists of the following components, in parts by weight: 60-200 parts of angelica sinensis, 50-180 parts of Millettia spatholobi, 50-150 parts of Ligusticum chuanxiong, 30-80 parts of mother of pearl, 40-100 parts of Uncaria rhynchophylla, 30-120 parts of white peony root, 20-90 parts of Prunella vulgaris, 20-80 parts of Pueraria root, 10-50 parts of Scutellaria baicalensis, 5-45 parts of Anemarrhena asphodeloides, 10-100 parts of Bamboo Shavings, and 10-70 parts of Patchouli.

[0015] A Chinese medicinal preparation for treating vertigo, which is prepared by any of the above-mentioned methods for preparing a Chinese medicinal preparation for treating vertigo.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention performs ultrasound-assisted cellulose enzymatic hydrolysis and extraction on Pueraria root and Uncaria rhynchophylla to prepare fluid extract A, then performs microwave-assisted reflux extraction on Angelica sinensis, White Peony Root, Herba Scutellariae, Rhizoma Anemarrhenae and Rhizoma Anemarrhenae to prepare fluid extract B, and then mixes the fluid extract A and fluid extract B with Millettia spatholobi, Mother of Pearl, Rhizoma Chuanxiong and Herba Patchouli that are ground into powder to prepare a traditional Chinese medicine preparation. Since the traditional Chinese medicine preparation uses Angelica sinensis, Millettia spatholobi and Rhizoma Chuanxiong as monarch drugs, Mother of Pearl, Uncaria rhynchophylla, White Peony Root, Herba Prunellae and Pueraria root as ministerial drugs, Scutellariae baicalensis and Rhizoma Anemarrhenae as adjuvant drugs, and Rhizoma Anemarrhenae and Herba Patchouli as guiding drugs, it can promote blood circulation and remove blood stasis, improve insufficient blood supply to the heart and brain, improve dizziness and the like, thereby effectively treating vertigo.

[0017] 2. The bamboo shavings and Patchouli used in the present invention have a synergistic effect. When bamboo shavings and Patchouli are used at the same time, they can play the role of removing dampness and reducing turbidity, raising clearness, and improving dizziness. The synergy of the two can effectively improve the therapeutic effect of the prepared traditional Chinese medicine preparation on vertigo.

[0018] 3. The raw materials used in the present invention are all natural plant raw materials, do not contain any chemical components, are non-toxic and have no side effects, are highly safe, well tolerated, and have no adverse reactions. DETAILED DESCRIPTION

[0019] The following is a detailed description of a Chinese medicine preparation for treating vertigo and a preparation method provided by the present invention in conjunction with specific embodiments. At the same time, it is explained here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and those skilled in the art may also adopt other alternatives to implement some known technologies.

[0020] Embodiment 1: A method for preparing a Chinese medicine preparation for treating vertigo, comprising the following steps: S1: Crushing, grinding and powder making 50 parts of Millettia spatholobi, 30 parts of mother-of-pearl, 50 parts of Chuanxiong and 10 parts of Patchouli are placed in a grinding machine, crushed and ground, and then passed through an 80-mesh sieve to obtain Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder; S2: Preparation of fluid extract A by ultrasound-assisted enzymatic extraction Methanol, ethanol and deionized water were stirred and mixed evenly in a volume ratio of 1:2:5 to obtain a mixed solution, and then 20 parts of Pueraria root and 40 parts of Uncaria rhynchophylla were crushed respectively, and then added to the mixed solution in a solid-liquid ratio of 1g:30mL, and ultrasonically dispersed for 10min to obtain a mixed suspension A, and then 0.4wt% of cellulase was added to the mixed suspension A, and heated at 45°C for 1h while stirring for enzymolysis, and then the enzyme was inactivated at 90°C for 3min to obtain an enzymolysis solution, and finally, the enzymolysis solution was heated to 60°C and ultrasonically treated for 30min, and after vacuum filtration, the filtrate was placed in a concentrator for concentration until the volume of the filtrate was concentrated to 1 / 10 of the original volume to obtain a fluid extract A; S3: Preparation of fluid extract B by microwave-assisted heating and pressurized reflux extraction 60 parts of Chinese angelica, 30 parts of white peony root, 20 parts of selfheal, 10 parts of scutellaria, 5 parts of rhizoma anemarrhenae and 10 parts of bamboo shavings were crushed to obtain Chinese angelica powder, white peony root powder, selfheal powder, scutellaria powder, rhizoma anemarrhenae powder and bamboo shavings powder, then the Chinese angelica powder, white peony root powder, selfheal powder, scutellaria powder, rhizoma anemarrhenae powder and bamboo shavings powder were added into a microwave extractor, and deionized water was added at a material-liquid ratio of 1g:20mL, and the mixture was stirred and mixed to obtain a mixed suspension B. Subsequently, the microwave extractor was pressurized to 1MPa, and the mixed suspension was heated at 100℃. B was heated for 20 minutes, and then subjected to microwave treatment at a power of 200 W for 20 minutes, and then continued to be heated for 1 hour, and microwave-assisted heating and pressurized reflux extraction was performed, and after natural cooling to room temperature, a mixed extract was obtained, and then the mixed extract was filtered, and the filter residue was subjected to microwave-assisted heating and pressurized reflux extraction again, and after filtration, the filtrate was combined to obtain a mixed extract, and finally, the mixed extract was placed in a concentrator, and heated and concentrated until the volume of the mixed extract was concentrated to 1 / 10 of the original volume, to obtain a fluid extract B; S4: Mixing and preparation of traditional Chinese medicine preparations The above-mentioned Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder are added into a mixer together with the above-mentioned fluid extract A and the above-mentioned fluid extract B, and are fully mixed to obtain a mixed wet material. The mixed wet material is then placed in a freeze dryer for freeze drying, and then placed in a crushing grinder for crushing and grinding. After passing through an 80-mesh sieve, a mixed powder is obtained. Finally, the mixed powder is filled into hard capsules according to the required amount, and then packaged to obtain a Chinese medicine preparation.

[0021] Embodiment 2: A method for preparing a Chinese medicine preparation for treating vertigo, comprising the following steps: S1: Crushing, grinding and powder making 115 parts of Millettia spatholobi, 55 parts of mother-of-pearl, 100 parts of Chuanxiong and 40 parts of Patchouli are placed in a grinding and milling machine, and then sieved through an 80-mesh sieve to obtain Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder; S2: Preparation of fluid extract A by ultrasound-assisted enzymatic extraction Methanol, ethanol and deionized water were stirred and mixed evenly in a volume ratio of 1:3:6 to obtain a mixed solution, and then 50 parts of Pueraria root and 70 parts of Uncaria rhynchophylla were crushed respectively, and then added to the mixed solution in a solid-liquid ratio of 1g:35mL, and ultrasonically dispersed for 15min to obtain a mixed suspension A, and then 0.5wt% cellulase was added to the mixed suspension A, and heated at 50°C for 1.5h while stirring for enzymolysis, and then the enzyme was inactivated at 90°C for 4min to obtain an enzymolysis solution, and finally, the enzymolysis solution was heated to 65°C and ultrasonically treated for 35min, and after vacuum filtration, the filtrate was placed in a concentrator for concentration until the volume of the filtrate was concentrated to 1 / 10 of the original volume to obtain a fluid extract A; S3: Preparation of fluid extract B by microwave-assisted heating and pressurized reflux extraction 130 parts of Chinese angelica, 75 parts of white peony root, 55 parts of selfheal, 30 parts of scutellaria, 25 parts of rhizoma anemarrhenae and 55 parts of bamboo shavings were crushed to obtain Chinese angelica powder, white peony root powder, selfheal powder, scutellaria powder, rhizoma anemarrhenae powder and bamboo shavings powder, then the Chinese angelica powder, white peony root powder, selfheal powder, scutellaria powder, rhizoma anemarrhenae powder and bamboo shavings powder were added into a microwave extractor, and deionized water was added at a material-liquid ratio of 1g:25mL, and the mixture was stirred and mixed to obtain a mixed suspension B. Subsequently, the microwave extractor was pressurized to 2MPa, and the mixed suspension was heated at 110°C. B was heated for 25 min, and then subjected to microwave treatment at a power of 250 W for 25 min, and then continued to be heated for 1.5 h, and subjected to microwave-assisted heating and pressurized reflux extraction, and naturally cooled to room temperature to obtain a mixed extract, and then the mixed extract was filtered, and the filter residue was subjected to microwave-assisted heating and pressurized reflux extraction again, and after filtration, the filtrate was combined to obtain a mixed extract, and finally, the mixed extract was placed in a concentrator, and heated and concentrated until the volume of the mixed extract was concentrated to 1 / 10 of the original volume, to obtain a fluid extract B; S4: Mixing and preparation of traditional Chinese medicine preparations The above-mentioned Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder are added into a mixer together with the above-mentioned fluid extract A and the above-mentioned fluid extract B, and are fully mixed to obtain a mixed wet material. The mixed wet material is then placed in a freeze dryer for freeze drying, and then placed in a crushing grinder for crushing and grinding. After passing through an 80-mesh sieve, a mixed powder is obtained. Finally, the mixed powder is filled into hard capsules according to the required amount, and then packaged to obtain a Chinese medicine preparation.

[0022] Embodiment 3: A method for preparing a Chinese medicine preparation for treating vertigo, comprising the following steps: S1: Crushing, grinding and powder making 180 parts of Millettia spatholobi, 80 parts of mother-of-pearl, 150 parts of Chuanxiong and 70 parts of Patchouli are placed in a grinding and milling machine, and then sieved through an 80-mesh sieve to obtain Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder; S2: Preparation of fluid extract A by ultrasound-assisted enzymatic extraction Methanol, ethanol and deionized water were stirred and mixed evenly in a volume ratio of 1:4:7 to obtain a mixed solution, and then 80 parts of Pueraria root and 100 parts of Uncaria rhynchophylla were crushed respectively, and then added to the mixed solution in a solid-liquid ratio of 1g:40mL, and ultrasonically dispersed for 20min to obtain a mixed suspension A, and then 0.6wt% of cellulase was added to the mixed suspension A, and heated at 55°C for 2h while stirring for enzymolysis, and then the enzyme was inactivated at 95°C for 5min to obtain an enzymolysis solution, and finally, the enzymolysis solution was heated to 70°C and ultrasonically treated for 40min, and after vacuum filtration, the filtrate was placed in a concentrator for concentration until the volume of the filtrate was concentrated to 1 / 10 of the original volume to obtain a fluid extract A; S3: Preparation of fluid extract B by microwave-assisted heating and pressurized reflux extraction 200 parts of Chinese angelica, 120 parts of white peony root, 90 parts of selfheal, 50 parts of scutellaria, 45 parts of rhizoma anemarrhenae and 100 parts of bamboo shavings were crushed to obtain Chinese angelica powder, white peony root powder, selfheal powder, scutellaria powder, rhizoma anemarrhenae powder and bamboo shavings powder, then the Chinese angelica powder, white peony root powder, selfheal powder, scutellaria powder, rhizoma anemarrhenae powder and bamboo shavings powder were added into a microwave extractor, and deionized water was added at a material-liquid ratio of 1g:30mL, and the mixture was stirred and mixed to obtain a mixed suspension B. Subsequently, the microwave extractor was pressurized to 3MPa, and the mixture was heated at 100-120℃. The combined suspension B is heated for 30 minutes, and then subjected to microwave treatment at a power of 300 W for 30 minutes, and then continued to be heated for 2 hours, and subjected to microwave-assisted heating and pressurized reflux extraction, and naturally cooled to room temperature to obtain a mixed extract, and then the mixed extract is filtered, and the filter residue is subjected to microwave-assisted heating and pressurized reflux extraction again, and after filtration, the filtrate is combined to obtain a mixed extract, and finally, the mixed extract is placed in a concentrator, and heated and concentrated until the volume of the mixed extract is concentrated to 1 / 10 of the original volume, to obtain a fluid extract B; S4: Mixing and preparation of traditional Chinese medicine preparations The above-mentioned Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder are added into a mixer together with the above-mentioned fluid extract A and the above-mentioned fluid extract B, and are fully mixed to obtain a mixed wet material. The mixed wet material is then placed in a freeze dryer for freeze drying, and then placed in a crushing grinder for crushing and grinding. After passing through an 80-mesh sieve, a mixed powder is obtained. Finally, the mixed powder is filled into hard capsules according to the required amount, and then packaged to obtain a Chinese medicine preparation.

[0023] Comparative Example 1: This comparative example is basically the same as Example 1, except that Patchouli is replaced with an equal amount of Bambusa rugosa.

[0024] Comparative Example 2: This comparative example is basically the same as Example 1, except that the bamboo shavings are replaced with an equal amount of Patchouli.

[0025] Experimental Example 1: Case Source: A total of 300 patients admitted to the Department of Neurology of Baotou Central Hospital with the main symptoms of vascular dizziness and vertigo were selected. According to the inclusion and exclusion criteria, a total of 300 patients were enrolled, 6 patients were excluded due to various reasons, and finally 294 patients were enrolled and randomly divided into treatment group 1 (73 cases), treatment group 2 (73 cases), treatment group 3 (73 cases) and control group (75 cases).

[0026] Case inclusion criteria: (1) Vascular dizziness / vertigo is defined as: the main cause is ischemic stroke. Ischemic stroke includes cerebral infarction and transient ischemic attack. Common causes include cardioembolism, atherosclerosis and dissection. (2) Age range is 18-80 years old. (3) Onset time is within 48 hours.

[0027] Case exclusion criteria: (1) Patients with severe vertebrobasilar artery thrombosis (NIHSS ≥ 17 points, GCS ≤ 8 points). (2) Patients with severe dysfunction of other organs, such as acute myocardial infarction, heart failure, liver and kidney failure, severe infection, coagulation dysfunction, etc. (3) Patients who cannot cooperate with treatment and follow-up. (4) Patients receiving thrombolytic therapy; (5) Exclude diseases that cause dizziness and vertigo symptoms due to other causes.

[0028] Treatment: (1) The control group adopted basic treatment, using 450 mg of Xueshuantong added to 250 mL of 0.9% normal saline, and taking regular oral medications such as Minshilang, Sibelium, and drugs for controlling blood sugar and blood pressure. (2) Treatment group 1 took the Chinese medicine preparation prepared in Example 1 on top of the basic treatment regimen, treatment group 2 took the Chinese medicine preparation prepared in Comparative Example 1 on top of the basic treatment regimen, and treatment group 3 took the Chinese medicine preparation prepared in Comparative Example 2 on top of the basic treatment regimen, 3 capsules each time, 3 times a day, 14 days as a course of treatment.

[0029] Method for determining efficacy: Cure: Clinical symptoms disappear and the body returns to normal; Markedly effective: clinical symptoms are significantly alleviated and do not affect basic life; Ineffective: If the clinical symptoms are not significantly alleviated or aggravated, it is judged as ineffective.

[0030] Comparison of efficacy: After one course of treatment, the treatment effects of the four groups were determined according to the efficacy criteria as shown in Table 1.

[0031] Table 1: Comparison of treatment effects among the four groups

[0032] As can be seen from Table 1, the therapeutic effect of treatment group 1 is the most prominent, and the therapeutic effects of treatment groups 2, 3 and the control group are all lower than that of treatment group 1. It can be seen that the ultrasonic-assisted cellulose enzymatic hydrolysis and extraction of Pueraria lobata and Uncaria rhynchophylla are used to prepare fluid extract A, and then the microwave-assisted reflux extraction of Angelica sinensis, White Peony Root, Herba Prunellae, Baical Skullcap Root, Rhizoma Anemarrhenae and Rhizoma Anemarrhenae is used to prepare fluid extract B, and then the fluid extract A and fluid extract B are mixed with the ground Millettia spatholobi, Mother of Pearl, Rhizoma Chuanxiong and Herba Patchouli to prepare the traditional Chinese medicine preparation. Since the traditional Chinese medicine preparation uses Angelica sinensis, Millettia spatholobi and Rhizoma Chuanxiong as the main drugs, uses Mother of Pearl, Uncaria rhynchophylla, White Peony Root, Rhizoma Prunellae and Pueraria lobata as the ministerial drugs, uses Baical Skullcap Root and Rhizoma Anemarrhenae as the adjuvant drugs, and uses Rhizoma Anemarrhenae and Herba Patchouli as the guiding drugs, it can play the role of promoting blood circulation and removing blood stasis, improving insufficient blood supply to the heart and brain, and improving dizziness, thereby effectively treating vertigo; In addition, the difference between Treatment Group 2 and Treatment Group 3 and Treatment Group 1 is that in Treatment Group 2, Patchouli was replaced with an equal amount of Bamboo Shavings, that is, only Bamboo Shavings were used; in Treatment Group 3, Bamboo Shavings were replaced with an equal amount of Patchouli, that is, only Patchouli were used; while Treatment Group 1 used both Bamboo Shavings and Patchouli. From the therapeutic effects in Table 1, it can be seen that the therapeutic effect of Treatment Group 1 was higher than that of Treatment Group 2 and Treatment Group 3. It can be seen that Bamboo Shavings and Patchouli have a synergistic effect. When Bamboo Shavings and Patchouli are used at the same time, they can play the role of removing dampness and turbidity, raising clearness, and improving dizziness. The synergy of the two can effectively improve the therapeutic effect of the prepared Chinese medicine preparation on vertigo.

[0033] Experimental Example 2: Research subjects: 12 healthy volunteers; half male and half female.

[0034] Inclusion criteria: age 18-60 years old, body mass index 18.5-24.9, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cre), urea (Urea), fasting blood glucose (Glu), routine blood, urine and stool tests, electrocardiogram and other examination indicators within the normal range.

[0035] Exclusion criteria: pregnant or lactating women (menstrual period depends on the specific medication); patients with mental or physical disabilities due to important primary diseases; suspected or confirmed history of alcohol or drug abuse; those with low possibility of inclusion in the group (such as frailty); allergic constitution (to more than two drugs, food, pollen); those with diseases that may reduce compliance; other subjects who the researchers believe cannot be included in the group.

[0036] Exit criteria: poor compliance of the subjects, failure to take medication on time and in the prescribed dosage; use of other drugs or foods that affect tolerance judgment; subjects unwilling to continue the clinical trial and voluntarily requesting to withdraw; loss to follow-up.

[0037] Exclusion criteria: those who do not meet the inclusion criteria; those who meet the exclusion criteria; those who have not taken medication; those who have no records.

[0038] Medication method: Volunteers were divided into three groups, each with 4 people, including 2 males and 2 females. Group 1 was given 1 tablet of the Chinese medicine prepared in Example 1 each time, twice a day; Group 2 was given 2 tablets of the Chinese medicine prepared in Example 1 each time, twice a day; Group 3 was given 4 tablets of the Chinese medicine prepared in Example 1 each time, twice a day. After taking the medicine for 1 day, the subjects were observed for 48 hours. Re-examination was conducted on the 4th day.

[0039] Statistical methods: SPSS 25.0 statistical software was used for analysis. Count data were expressed as mean ± standard deviation. T test was used for comparison of two groups of data. Multiple comparisons were used for three or more groups of data. ''*'' or ''**'' indicates P < 0.05 or P < 0.01, indicating statistical significance, see Table 2-5 for details.

[0040] Table 2: Comparison of indicators of volunteers in group 1 before and after taking the medicine AST(U / L) ALT(U / L) Cre (umol / L) Urea (mmol / L) Glu (mmol / L) <![CDATA[WBC(10 9 / L)]]> <![CDATA[RBC(10 12 / L)]]> <![CDATA[PLT(10 9 / L)]]> Urinalysis abnormality ECG abnormalities Before taking medication 29.02±1.02 22.02±1.02 78.32±5.43 7.05±0.87 5.22±0.47 6.50±0.32 4.38±0.99 191.56±22.67 0 0 After taking the medicine 28.04±0.99 21.30±1.05 81.04±5.82 7.11±0.79 4.98±0.35 6.03±0.55 4.69±0.48 208.44±20.71 0 0 Table 3: Comparison of indicators of volunteers in group 2 before and after taking the medicine AST(U / L) ALT(U / L) Cre (umol / L) Urea (mmol / L) Glu (mmol / L) <![CDATA[WBC(10 9 / L)]]> <![CDATA[RBC(10 12 / L)]]> <![CDATA[PLT(10 9 / L)]]> Urinalysis abnormality ECG abnormalities Before taking medication 31.45±1.68 23.28±0.95 80.25±3.43 6.84±0.36 4.98±0.21 7.03±0.47 4.29±0.85 216.43±21.89 0 0 After taking the medicine 29.04±0.85 22.45±0.85 81.76±4.31 7.09±0.63 5.32±0.47 6.55±0.54 4.04±0.51 209.40±21.48 0 0 Table 4: Comparison of indicators of volunteers in group 3 before and after taking the medicine AST(U / L) ALT(U / L) Cre (umol / L) Urea (mmol / L) Glu (mmol / L) <![CDATA[WBC(10 9 / L)]]> <![CDATA[RBC(10 12 / L)]]> <![CDATA[PLT(10 9 / L)]]> Urinalysis abnormality ECG abnormalities Before taking medication 30.11±2.67 24.26±1.11 77.38±4.37 6.83±0.45 4.92±0.25 5.98±0.25 4.00±0.32 189.32±20.48 0 0 After taking the medicine 29.38±1.03 23.09±1.26 79.25±4.36 6.70±0.52 5.06±0.29 6.12±0.47 4.32±0.35 202.42±18.23 0 0 Table 5: Comparison of indicators of the three groups of volunteers after taking the medicine AST(U / L) ALT(U / L) Cre (umol / L) Urea (mmol / L) Glu (mmol / L) <![CDATA[WBC(10 9 / L)]]> <![CDATA[RBC(10 12 / L)]]> <![CDATA[PLT(10 9 / L)]]> Urinalysis abnormality rate ECG abnormality rate 1 group 28.04±0.99 21.30±1.05 81.04±5.82 7.11±0.79 4.98±0.35 6.03±0.55 4.69±0.48 208.44±20.71 0 0 2 groups 29.04±0.85 22.45±0.85 81.76±4.31 7.09±0.63 5.32±0.47 6.55±0.54 4.04±0.51 209.40±21.48 0 0 3 groups 29.38±1.03 23.09±1.26 79.25±4.36 6.70±0.52 5.06±0.29 6.12±0.47 4.32±0.35 202.42±18.23 0 0 As can be seen from Tables 2-5, there was no statistically significant difference in the main biochemical indicators before and after medication in each group (P>0.05), indicating that the Chinese medicine preparation prepared in Example 1 had no toxic side effects on healthy people and had good safety; pairwise comparisons of biochemical indicators after medication in each group showed no statistically significant difference (P>0.05), indicating that after healthy people took three doses of Chinese medicine preparations, no toxic side effects were produced to the body and the tolerance to the drugs was good.

[0041] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for preparing a Chinese medicine preparation for treating vertigo, characterized in that: The steps include: S1: Crushing, grinding and powder making Place Millettia spatholobi, mother-of-pearl, Chuanxiong herb and Patchouli in a grinding and milling machine, grind and grind them, and then pass them through an 80-mesh sieve to obtain Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong herb powder and Patchouli powder; S2: Preparation of fluid extract A by ultrasound-assisted enzymatic extraction Methanol, ethanol and deionized water are prepared into a mixed solution for later use, and Pueraria root and Uncaria rhynchophylla are crushed respectively and added into the mixed solution for uniform dispersion, and then cellulase is added, and heated for enzymolysis to inactivate the enzyme, and then ultrasonic treatment is performed, and the fluid extract A is obtained by suction filtration and concentration; S3: Preparation of fluid extract B by microwave-assisted heating and pressurized reflux extraction The Chinese angelica, white peony root, Prunella vulgaris, scutellaria baicalensis, Rhizoma Anemarrhenae and Rhizoma Anemarrhenae were ground and mixed with deionized water, and then subjected to microwave-assisted heating and pressurized reflux extraction, and filtered and concentrated to obtain fluid extract B; S4: Mixing and preparation of traditional Chinese medicine preparations The above-mentioned Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder are uniformly mixed with the above-mentioned fluid extract A and fluid extract B, and then freeze-dried, pulverized, ground, sieved, filled and packaged to obtain a traditional Chinese medicine preparation.

2. The method for preparing a Chinese medicine preparation for treating vertigo according to claim 1, characterized in that: S2 specifically includes the following steps: S2.1: Methanol, ethanol and deionized water are stirred and mixed uniformly in a volume ratio of 1:(2-4):(5-7) to obtain a mixed solution; S2.2: Grind Puerariae Radix and Uncaria Rhynchophylla Rhizome separately, add them into the above mixed solution at a solid-liquid ratio of 1g:(30-40)mL, and disperse them by ultrasonic for 10-20min to obtain a mixed suspension A; S2.3: Add cellulase to the mixed suspension A, heat at 45-55°C for 1-2h while stirring to perform enzymolysis, and then inactivate the enzyme for 3-5min to obtain an enzymolysis solution; S2.4: Heat the above enzymatic hydrolysate to 60-70°C and perform ultrasonic treatment for 30-40 minutes. After vacuum filtration, place the filtrate in a concentrator for concentration until the volume of the filtrate is concentrated to 1 / 10 of the original volume to obtain fluid extract A.

3. The method for preparing a Chinese medicine preparation for treating vertigo according to claim 2, characterized in that: S3 specifically includes the following steps: S3.1: Grind angelica sinensis, white peony root, selfheal, scutellaria baicalensis, anemarrhena asphodeloides and bamboo shavings respectively to obtain angelica sinensis powder, white peony root powder, selfheal powder, scutellaria baicalensis powder, anemarrhena asphodeloides powder and bamboo shavings powder; S3.2: Add the above-mentioned angelica powder, white peony powder, selfheal powder, scutellaria powder, anemarrhena powder and bamboo shavings powder into a microwave extractor, and then add deionized water at a material-liquid ratio of 1g: (20-30)mL, stir and mix evenly to obtain a mixed suspension B; S3.3: pressurizing the microwave extractor, heating the mixed suspension B at 100-120°C for 20-30 minutes, subjecting it to microwave treatment, and then continuing to heat for 1-2 hours, subjecting it to microwave-assisted heating and pressurized reflux extraction, and naturally cooling it to room temperature to obtain a mixed extract; S3.4: filtering the mixed extract, and subjecting the residue to microwave-assisted heating and pressurized reflux extraction again, filtering, combining the filtrates, and obtaining a mixed extract; S3.5: Place the mixed extract in a concentrator and heat and concentrate until the volume of the mixed extract is concentrated to 1 / 10 of the original volume, thereby obtaining fluid extract B.

4. The method for preparing a Chinese medicine preparation for treating vertigo according to claim 3, characterized in that: S4 specifically includes the following steps: S4.1: Add the Millettia spatholobi powder, mother-of-pearl powder, Chuanxiong powder and Patchouli powder prepared in step S1, the fluid extract A prepared in step S2.4 and the fluid extract B prepared in step S3.5 into a mixer, mix them thoroughly and evenly to obtain a mixed wet material; S4.2: placing the above mixed wet material in a freeze dryer for freeze drying, and then placing it in a crushing and grinding machine for crushing and grinding, and passing through an 80-mesh sieve to obtain a mixed powder; S4.3: Fill the above mixed powder into hard capsules according to the required amount, and then package them to obtain a Chinese medicine preparation.

5. The method for preparing a Chinese medicine preparation for treating vertigo according to claim 2, characterized in that: The added amount of cellulase is 0.4-0.6wt%.

6. The method for preparing a Chinese medicine preparation for treating vertigo according to claim 2, characterized in that: The enzyme inactivation temperature is 90-95°C.

7. The method for preparing a Chinese medicine preparation for treating vertigo according to claim 3, characterized in that: The pressure of microwave-assisted heating and pressurized extraction is 1-3MPa.

8. The method for preparing a Chinese medicine preparation for treating vertigo according to claim 3, characterized in that: The microwave treatment power is 200-300W and the treatment time is 20-30min.

9. The method for preparing a Chinese medicine preparation for treating vertigo according to claim 4, characterized in that: The Chinese medicine preparation is composed of the following components by weight: 60-200 parts of angelica sinensis, 50-180 parts of Millettia spatholobi, 50-150 parts of Chuanxiong, 30-80 parts of mother of pearl, 40-100 parts of Uncaria rhynchophylla, 30-120 parts of white peony root, 20-90 parts of Prunella vulgaris, 20-80 parts of Pueraria root, 10-50 parts of Scutellaria baicalensis, 5-45 parts of Anemarrhena asphodeloides, 10-100 parts of Bambusa rhizoma, and 10-70 parts of Patchouli.

10. A Chinese medicine preparation for treating vertigo, characterized in that: The preparation is prepared by the method for preparing a traditional Chinese medicine preparation for treating vertigo as described in any one of claims 1 to 9.

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