Traditional Chinese medicine preparation for treating hepatopathy and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine preparation for treating hepatopathy and a preparation method thereof, and relates to the technical field of traditional Chinese medicines. According to the traditional Chinese medicine preparation for treating liver diseases and the preparation method thereof, the traditional Chinese medicine preparation comprises the following components in parts by weight: 20-35 parts of cordyceps militaris, 10-25 parts of radix puerariae, 10-18 parts of rhizoma smilacis glabrae, 5-12 parts of prismatomeris tetrandra, 15-25 parts of oxtail, 5-8 parts of lucid ganoderma, 12-24 parts of schisandra chinensis, 4-9 parts of hovenia dulcis thunb and 20-25 parts of salvia miltiorrhiza. The traditional Chinese medicine has a remarkable effect on treating viral hepatitis and alcoholic hepatitis, also plays a role in maintaining the spleen and kidney of a patient in the process of treating liver diseases, has no toxic or side effect after being used for a long time, has the effects of reducing the fat content of the liver and improving the lipid metabolism capability of the liver, and also can play the effects of protecting and nourishing the liver before and after drinking; meanwhile, the added components such as cordyceps militaris, the root of kudzu vine, the root of red-rooted salvia, lucid ganoderma and the like are cooperated to play a certain role in blocking and preventing liver canceration of a user.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine preparation for treating liver diseases and a preparation method thereof. Background Art

[0002] Common symptoms of liver disease include: fatigue, side pain, poor appetite, abdominal distension, bitter taste in the mouth, yellow urine, loose stools, poor sleep, dry eyes, dizziness, yellow tongue coating, greasy tongue coating, dark red tongue coating, cracked tongue, purple tongue, pale red tongue with tongue tooth marks, pale and fat tongue, stringy or thin pulse, dry mouth, dry eyes, yellow eyes, yellow body, etc.

[0003] Common pathogenesis of liver disease: invasion of damp-heat evil, liver depression and spleen deficiency, accumulation of damp-heat, qi stagnation and blood stasis, damp-heat obstruction in the middle, liver-stomach disharmony, liver-kidney yin deficiency, etc. Among them, damp-heat evil is the main cause, damp-heat accumulation or obstruction in the middle jiao, and qi stagnation and blood stasis are pathological factors. The treatment methods of liver disease are relatively rich, and the combination of Chinese and Western medicine has significant effects. Chinese medicine treatment emphasizes the combination of disease differentiation and syndrome differentiation, the use of strengthening the body and eliminating evil at the same time, and the treatment of both the symptoms and the root causes. The treatment should be continuous and long-term, and intermittent treatment is avoided. Western medicine mainly focuses on protecting the liver, protecting the liver, anti-virus and treating corresponding complications. The following is a brief discussion of the five commonly used methods of Chinese medicine to treat liver disease.

[0004] Clearing away heat and detoxifying, strengthening the spleen and removing dampness, soothing the liver and relieving depression, and nourishing the liver and kidneys are important reasons for treating and preventing the occurrence of liver disease. Therefore, the treatment of liver disease focuses on clearing away heat and detoxifying. External damp-heat evil toxins are combined with endogenous damp-heat, and the spleen is strengthened to prevent the spread of liver disease, assist in nourishing kidney yin, and soften the liver. Therefore, it is necessary to protect and maintain the liver and maintain the liver and spleen while treating liver disease with Chinese medicine preparations. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides a Chinese medicine preparation for treating liver disease and a preparation method thereof, which solves the problems raised in the above-mentioned background technology.

[0006] To achieve the above objectives, the present invention is implemented through the following technical scheme. A traditional Chinese medicine preparation for treating liver disease and a preparation method thereof include the following components in parts by weight: 20-35 parts of Cordyceps militaris, 10-25 parts of Pueraria root, 10-18 parts of Smilax glabra, 5-12 parts of Xanthoceras chinensis, 15-25 parts of Oxtail Herb, 5-8 parts of Ganoderma lucidum, 12-24 parts of Schisandra chinensis, 4-9 parts of Hovenia dulcis, and 20-25 parts of Salvia miltiorrhiza.

[0007] The pharmacological effects of the components used in the treatment of liver diseases are further disclosed herein:

[0008] Cordyceps militaris is rich in vitamins, minerals, proteins, amino acids and other ingredients, and has a high tonic effect. Taking it in moderation can strengthen the body's body and improve its immunity. Therefore, cordycepic acid and cordycepin in Cordyceps militaris can dilate and soften blood vessels and lower blood pressure. Cordyceps polysaccharides can also improve the liver's detoxification ability, thus playing a role in lowering blood pressure and protecting the liver.

[0009] Pueraria root contains soybean glycosides, which can decompose the alcohol content in the blood, reduce liver fat content, improve liver lipid metabolism, and has the effects of scavenging oxygen free radicals and resisting lipid peroxidation, which can protect the liver, improve the energy metabolism of liver cells, and prevent liver cell damage.

[0010] Smilax glabra can assist in the treatment of liver cirrhosis by promoting diuresis, reducing swelling, detoxifying, strengthening the spleen and stomach, relaxing muscles and tendons, and promoting blood circulation, thereby preventing liver cell necrosis and achieving antibacterial effects.

[0011] Because the root of yellow pine contains 2-methylanthraquinone, methyl isorubiacin, methyl isorubiacin-1-methyl ether, thorn aldehyde, sitosterol, anthraquinone glycoside compounds and other ingredients, and it has a cooling effect and can relieve heat, it has a significant effect in relieving hepatitis.

[0012] Oxtail grass contains oleanolic acid and ursolic acid, which can play an anti-hepatitis, liver protection, liver protection, and liver detoxification effect. It can play an auxiliary therapeutic effect in the treatment of chronic viral hepatitis and relieve the condition. Therefore, it plays an auxiliary role in the treatment of liver disease.

[0013] Lingzhi contains polysaccharides and nucleosides, which can repair damaged liver cells, promote liver cell regeneration, and restore liver function. Lingzhi can also increase the activity of SOD enzymes in patients, eliminate free radicals, and block their damage to liver cells.

[0014] Hovenia dulcis contains rich water and organic acids, which can promote alcohol metabolism and reduce the time alcohol stays in the body, thereby preventing liver damage, enhancing the ability of liver cells to resist damage, and helping to prevent and improve liver disease;

[0015] The main components of Danshen, tanshinone and bioactive substances, can improve the activity of the patient's body cells on the one hand, and on the other hand, similar to Pueraria root, remove excess free radicals in the body to prevent free radical peroxidation, improve the energy metabolism of liver cells, and prevent liver cell damage;

[0016] Therefore, in summary, the Chinese medicine preparation for treating liver disease of the present application has significant effects on the treatment of viral hepatitis and alcoholic hepatitis, and also plays a role in maintaining the spleen and kidneys of patients in the process of treating liver disease. It has no toxic side effects when used for a long time, and reduces the fat content in the liver and improves the lipid metabolism capacity of the liver. It can also protect and nourish the liver before and after drinking. At the same time, the added components such as Cordyceps sinensis, Pueraria lobata, Salvia miltiorrhiza and Ganoderma lucidum cooperate synergistically to play a certain blocking and preventing effect on liver cancer in users.

[0017] A further improvement of the technical solution of the present invention is that its preparation forms include injection, powder injection, freeze-dried powder injection, tablets, capsules, granules, oral solution, pills or sustained-release preparations.

[0018] A further improvement of the technical solution of the present invention is that a method for preparing a traditional Chinese medicine preparation for treating liver disease is also provided, wherein the pretreatment of Pueraria lobata granules comprises the following specific steps:

[0019] S1: Weigh the kudzu root according to the formula, cut the kudzu root to be used into slices and dry them, add 500 ml of water to the dried kudzu root into a decoction pot, decoct for 1.5 hours, and repeat the decoction for 3 times until the kudzu root decoction in the pot is clear, filter the obtained medicinal liquid under negative pressure, concentrate it under reduced pressure at an ambient temperature of 50°C to a viscous state, place it in a drying oven for vacuum drying, and finally grind the obtained product to obtain kudzu root extract powder.

[0020] A further improvement of the technical solution of the present invention is that the smilax glabra, yellow root and oxtail grass are uniformly pre-treated, including the following specific steps:

[0021] Q1: Weigh smilax glabra, yellow root and oxtail grass according to the formula, mix them and add them into a treatment tank filled with an appropriate amount of water, extract them under slight boiling reflux at 48°C, and centrifuge the extract at 3000r / min for 5min. Filter the supernatant with a Buchner funnel, and dry it in a drying oven under vacuum. Finally, grind the product to obtain mixed extract powder A.

[0022] A further improvement of the technical solution of the present invention is that the Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum are uniformly pretreated as particles, comprising the following specific steps:

[0023] T1: According to the formula, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum were weighed and ground into coarse powder in a grinder. 80%-90% ethanol was added and immersed for 24 hours. After reflux extraction for 3 times, the obtained liquid was filtered under negative pressure, and concentrated under reduced pressure at an ambient temperature of 50°C to a viscous state. An appropriate amount of water was added, heated at 30-60°C, stirred and dissolved, and the ethanol was recovered, concentrated and dried to obtain mixed extract powder B.

[0024] A further improvement of the technical solution of the present invention is that the pretreatment of the kudzu root solution comprises the following specific steps:

[0025] S2: Weigh Pueraria root according to the formula, add the corresponding amount of water for reflux extraction, centrifuge the extract for 10 minutes, filter the supernatant with a Buchner funnel, collect and filter through a 0.3um microporous filter membrane, discard the initial filtrate to obtain the Pueraria root solution.

[0026] A further improvement of the technical solution of the present invention is that the smilax glabra, yellow root and oxtail grass are pretreated with a unified solution, comprising the following specific steps:

[0027] Q2: Weigh Smilax glabra, Rhizoma Coptidis and Herba Cynoglossi according to the formula, add corresponding times of water for reflux extraction, centrifuge the extract for 10 minutes, filter the supernatant with a Buchner funnel, collect and filter through a 0.3um microporous filter membrane, discard the initial filtrate to obtain mixed solution A.

[0028] A further improvement of the technical solution of the present invention is that Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum are uniformly pretreated with a solution, comprising the following specific steps:

[0029] T2: Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum were weighed according to the formula, and the corresponding amount of water was added for reflux extraction. The extract was centrifuged for 10 minutes, and the supernatant was filtered with a Buchner funnel. After collection, the supernatant was filtered through a 0.3um microporous membrane, and the initial filtrate was discarded to obtain a mixed solution B.

[0030] A further improvement of the technical solution of the present invention is that the preparation of the powder injection, freeze-dried powder injection, tablet, capsule and granule of the traditional Chinese medicine comprises the following specific steps: taking the kudzu root extract powder, mixed extract powder A and mixed extract powder B obtained by S1, Q1 and T1 and fully mixing them, further heating and drying them at 63°C, adding cross-linked starch at a mass ratio of 1:1, using an appropriate amount of 75°C ethanol to make a soft material, reducing the pressure at 60°C for 24 hours, filtering and sieving with a sieve to obtain traditional Chinese medicine preparation particles, and further processing them to obtain freeze-dried powder injection, tablets, capsules and granules;

[0031] The preparation of injection, oral liquid, pill and sustained-release preparation includes the following specific steps: taking the kudzu root solution, mixed solution A and mixed solution B obtained from S2, Q2 and T2, mixing them, stirring while heating until uniform, cooling to room temperature, and further processing to obtain injection, oral liquid, pill and sustained-release preparation.

[0032] Compared with the prior art, the beneficial effects of the present invention are that it is significantly effective in treating viral hepatitis and alcoholic hepatitis, and it also maintains the spleen and kidneys of patients during the treatment of liver disease. It has no toxic side effects when used for a long time, and it can reduce the fat content in the liver and improve the lipid metabolism capacity of the liver. It can also protect and nourish the liver before and after drinking. At the same time, the added components such as Cordyceps sinensis, Pueraria lobata, Salvia miltiorrhiza and Ganoderma lucidum cooperate synergistically to play a certain blocking and preventing role in liver cancer in users. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 A schematic diagram of the preparation of granule preparations in the preparation method of traditional Chinese medicine for treating liver diseases;

[0034] Figure 2 This is the overall SOD and MDA data table of Experiments A, B, and C in Example 1 after 12 weeks;

[0035] Figure 3 This is a table of various liver function test data of 40 clinical staff in Example 2;

[0036] Figure 4 This is a schematic diagram of the preparation of liquid preparations in the method for preparing traditional Chinese medicine preparations for treating liver diseases. DETAILED DESCRIPTION

[0037] Various exemplary embodiments, features and aspects of the present application will be described in detail below with reference to the accompanying drawings. The same reference numerals in the accompanying drawings represent elements with the same or similar functions. Although various aspects of the embodiments are shown in the accompanying drawings, the drawings are not necessarily drawn to scale unless otherwise specified.

[0038] The word “exemplary” is used exclusively herein to mean “serving as an example, example, or illustration.” Any embodiment described herein as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments.

[0039] In addition, in order to better illustrate the present application, numerous specific details are provided in the specific embodiments below. It should be understood by those skilled in the art that the present application can also be implemented without certain specific details. In some examples, methods, means, and elements well known to those skilled in the art are not described in detail in order to highlight the subject matter of the present application.

[0040] The invention provides a traditional Chinese medicine preparation for treating liver disease and a preparation method thereof. The traditional Chinese medicine preparation comprises the following components in parts by weight: 20-35 parts of cordyceps militaris, 10-25 parts of kudzu root, 10-18 parts of smilax glabra, 5-12 parts of yellow root, 15-25 parts of oxtail grass, 5-8 parts of ganoderma lucidum, 12-24 parts of schisandra chinensis, 4-9 parts of hovenia dulcis, and 20-25 parts of salvia miltiorrhiza.

[0041] The pharmacological effects of the components used in the treatment of liver diseases are further disclosed herein:

[0042] Cordyceps militaris is rich in vitamins, minerals, proteins, amino acids and other ingredients, and has a high tonic effect. Taking it in moderation can strengthen the body's body and improve its immunity. Therefore, cordycepic acid and cordycepin in Cordyceps militaris can dilate and soften blood vessels and lower blood pressure. Cordyceps polysaccharides can also improve the liver's detoxification ability, thus playing a role in lowering blood pressure and protecting the liver.

[0043] Pueraria root contains soybean glycosides, which can decompose the alcohol content in the blood, reduce liver fat content, improve liver lipid metabolism, and has the effects of scavenging oxygen free radicals and resisting lipid peroxidation, which can protect the liver, improve the energy metabolism of liver cells, and prevent liver cell damage.

[0044] Smilax glabra can assist in the treatment of liver cirrhosis by promoting diuresis, reducing swelling, detoxifying, strengthening the spleen and stomach, relaxing muscles and tendons, and promoting blood circulation, thereby preventing liver cell necrosis and achieving antibacterial effects.

[0045] Because the root of yellow pine contains 2-methylanthraquinone, methyl isorubiacin, methyl isorubiacin-1-methyl ether, thorn aldehyde, sitosterol, anthraquinone glycoside compounds and other ingredients, and it has a cooling effect and can relieve heat, it has a significant effect in relieving hepatitis.

[0046] Oxtail grass contains oleanolic acid and ursolic acid, which can play an anti-hepatitis, liver protection, liver protection, and liver detoxification effect. It can play an auxiliary therapeutic effect in the treatment of chronic viral hepatitis and relieve the condition. Therefore, it plays an auxiliary role in the treatment of liver disease.

[0047] Lingzhi contains polysaccharides and nucleosides, which can repair damaged liver cells, promote liver cell regeneration, and restore liver function. Lingzhi can also increase the activity of SOD enzymes in patients, eliminate free radicals, and block their damage to liver cells.

[0048] Hovenia dulcis contains rich water and organic acids, which can promote alcohol metabolism and reduce the time alcohol stays in the body, thereby preventing liver damage, enhancing the ability of liver cells to resist damage, and helping to prevent and improve liver disease;

[0049] The main components of Danshen, tanshinone and bioactive substances, can improve the activity of the patient's body cells on the one hand, and on the other hand, similar to Pueraria root, remove excess free radicals in the body to prevent free radical peroxidation, improve the energy metabolism of liver cells, and prevent liver cell damage;

[0050] Therefore, in summary, the Chinese medicine preparation for treating liver disease of the present application has significant effects on the treatment of viral hepatitis and alcoholic hepatitis, and also plays a role in maintaining the spleen and kidneys of patients in the process of treating liver disease. It has no toxic side effects when used for a long time, and reduces the fat content in the liver and improves the lipid metabolism capacity of the liver. It can also protect and nourish the liver before and after drinking. At the same time, the added components such as Cordyceps sinensis, Pueraria lobata, Salvia miltiorrhiza and Ganoderma lucidum cooperate synergistically to play a certain blocking and preventing effect on liver cancer in users.

[0051] Including injection, powder injection, lyophilized powder injection, tablets, capsules, granules, oral solution, pills or sustained-release preparations.

[0052] The present application also provides a specific preparation method of a Chinese medicine preparation applied to the above components, wherein the pretreatment of Pueraria lobata particles comprises the following specific steps:

[0053] S1: Weigh the kudzu root according to the formula, cut the kudzu root to be used into slices and dry them, add 500 ml of water to the dried kudzu root into a decoction pot, decoct for 1.5 hours, and repeat the decoction for 3 times until the kudzu root decoction in the pot is clear, filter the obtained medicinal liquid under negative pressure, concentrate it under reduced pressure at an ambient temperature of 50°C to a viscous state, place it in a drying oven for vacuum drying, and finally grind the obtained product to obtain kudzu root extract powder.

[0054] Among them, the smilax glabra, yellow root and oxtail grass are uniformly pre-treated, including the following specific steps:

[0055] Q1: Weigh smilax glabra, yellow root and oxtail grass according to the formula, mix them and add them into a treatment tank filled with an appropriate amount of water, extract them under slight boiling reflux at 48°C, and centrifuge the extract at 3000r / min for 5min. Filter the supernatant with a Buchner funnel, and dry it in a drying oven under vacuum. Finally, grind the product to obtain mixed extract powder A.

[0056] The Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum are uniformly pre-treated, including the following specific steps:

[0057] T1: According to the formula, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum were weighed and ground into coarse powder in a grinder. 80%-90% ethanol was added and immersed for 24 hours. After reflux extraction for 3 times, the obtained liquid was filtered under negative pressure, and concentrated under reduced pressure at an ambient temperature of 50°C to a viscous state. An appropriate amount of water was added, heated at 30-60°C, stirred and dissolved, and the ethanol was recovered, concentrated and dried to obtain mixed extract powder B.

[0058] Wherein the Pueraria solution pretreatment comprises the following specific steps:

[0059] S2: Weigh Pueraria root according to the formula, add the corresponding amount of water for reflux extraction, centrifuge the extract for 10 minutes, filter the supernatant with a Buchner funnel, collect and filter through a 0.3um microporous filter membrane, discard the initial filtrate to obtain the Pueraria root solution.

[0060] Among them, the Chinese smilax glabra, yellow root and oxtail grass are pretreated with a unified solution, including the following specific steps:

[0061] Q2: Weigh Smilax glabra, Rhizoma Coptidis and Herba Cynoglossi according to the formula, add corresponding times of water for reflux extraction, centrifuge the extract for 10 minutes, filter the supernatant with a Buchner funnel, collect and filter through a 0.3um microporous filter membrane, discard the initial filtrate to obtain mixed solution A.

[0062] The Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum are uniformly pretreated with the solution, including the following specific steps:

[0063] T2: Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum were weighed according to the formula, and the corresponding amount of water was added for reflux extraction. The extract was centrifuged for 10 minutes, and the supernatant was filtered with a Buchner funnel. After collection, the supernatant was filtered through a 0.3um microporous membrane, and the initial filtrate was discarded to obtain a mixed solution B.

[0064] The preparation of the powder injection, freeze-dried powder injection, tablet, capsule and granule of the Chinese medicine comprises the following specific steps: taking the kudzu root extract powder, mixed extract powder A and mixed extract powder B obtained from S1, Q1 and T1 and fully mixing them, further heating and drying them at 63°C, adding cross-linked starch at a mass ratio of 1:1, using an appropriate amount of 75°C ethanol to make a soft material, reducing the pressure at 60°C for 24 hours, filtering and sieving with a sieve to obtain Chinese medicine preparation particles, and further treating them to obtain freeze-dried powder injection, tablets, capsules and granules;

[0065] The preparation of injection, oral liquid, pill and sustained-release preparation includes the following specific steps: taking the kudzu root solution, mixed solution A and mixed solution B obtained from S2, Q2 and T2, mixing them, stirring while heating until uniform, cooling to room temperature, and further processing to obtain injection, oral liquid, pill and sustained-release preparation.

[0066] Example 1: In this example, 4 groups of control experiments were set up to explore and verify the effect of the Chinese medicine preparation proposed in this application on the treatment of liver disease, and KM mice were selected as experimental carriers;

[0067] Experimental group A selected traditional Chinese medicine preparations and 36 experimental mice. Preparation of traditional Chinese medicine preparations Pueraria extract powder, mixed extract powder A and mixed extract powder B obtained from S1, Q1 and T1 were fully mixed, further heated and dried at 63°C, and cross-linked starch was added at a mass ratio of 1:1. A proper amount of 75°C ethanol was used to make a soft material, and the mixture was decompressed at 60°C for 24h. The filter sieve was used for double screening to obtain traditional Chinese medicine preparation granules. The traditional Chinese medicine granules were mixed in the experimental mouse food to ensure that there was no residue each time it was eaten;

[0068] Experimental group B, no therapeutic drugs were selected, with 36 experimental mice;

[0069] Experiment C group selected a preparation prepared with Smilax glabra, yellow root and cowtail grass as the basic components. The preparation was prepared by weighing Smilax glabra, yellow root and cowtail grass, mixing them into a treatment tank filled with an appropriate amount of water, extracting them under a slight boiling reflux at 48°C, and centrifuging the extract at 3000r / min for 5min. The supernatant was filtered with a Buchner funnel and placed in a drying oven for vacuum drying. The final product was crushed and mixed into the food of the experimental mice to ensure that there was no residue each time it was eaten.

[0070] All three experimental groups were gavaged with 30% edible alcohol, with the specific amount controlled at 0.5±0.1ml, twice a day, once before meals and once after meals. After continuous gavage for 4, 8, and 12 weeks, 12 mice were killed for testing indicators.

[0071] In the experimental group D, no therapeutic drugs were used. 36 experimental mice were gavaged with normal saline. The specific amount was controlled at 0.5±0.1ml, twice a day, once before meals and once after meals. After continuous gavage for 4 weeks, 8 weeks, and 12 weeks, 12 mice were killed and the indicators were tested.

[0072] The SOD activity and MAD content in the liver tissue of the sacrificed experimental mice were detected, and the liver tissue of the experimental mice was fixed with 4% paraformaldehyde for 24 hours, routine tissue sections were made, HE staining was performed, and observation was performed under a light microscope.

[0073] The results of the experimental group B were as follows: the liver was enlarged and grayish yellow, the cytoplasm of the liver cells was loose at 4 weeks, and there were a small number of lipid droplets and vacuoles in the cytoplasm; at 8 weeks, the liver cells of the experimental mice were arranged in disorder, and there were a large number of lipid droplets and vacuoles in the cytoplasm; at 12 weeks, a large number of liver cells were degenerated, and lipid droplets and vacuoles accounted for more than 85% of all cells in the entire field of view, and the proportion of fatty degeneration cells was significantly higher than that of groups A, C, and D.

[0074] The observation results of experimental group A were as follows: at 4 weeks, the surface of the experimental mouse liver was smooth and dark red, and the liver lobule structure was complete and clear under the microscope; at 12 weeks, the surface of the mouse liver was smooth and slightly yellowish red, and a small amount of lipid droplets and vacuoles were visible in the cytoplasm;

[0075] The results of the experimental group C were as follows: at 4 weeks, the surface of the experimental rat liver was smooth and slightly yellowish red, and the liver lobules were slightly blurred under the microscope; at 12 weeks, the surface of the rat liver was smooth and slightly yellowish, and the proportion of fatty cells was significantly higher than that of group A and lower than that of group B;

[0076] The observation results of experimental group D were as follows: the surface of the experimental mouse liver was smooth and dark red, and under the microscope, the liver lobule structure was complete and clear, the liver cells were arranged in hepatic cords, the cells were polygonal and of relatively uniform size.

[0077] Example 2: A clinical trial of alcoholic hepatitis was conducted using the above-mentioned Chinese medicine preparation for treating liver disease. There were 20 men and women, with an average age of 25-60 years old. The medicine was taken once after breakfast, lunch and dinner for 1 month. After taking the medicine, the patients' serum albumin, globulin, γ-globulin, A / G, and serum ALT all returned to normal, all meeting the standard of marked efficacy. No obvious adverse reactions were found in the follow-up observation of these 40 people. The results are shown in the attached figure.

[0078] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0079] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese medicine preparation for treating liver disease, characterized in that: The invention comprises the following components in parts by weight: 20-35 parts of cordyceps militaris, 10-25 parts of kudzu root, 10-18 parts of smilax glabra, 5-12 parts of yellow root, 15-25 parts of oxtail grass, 5-8 parts of ganoderma lucidum, 12-24 parts of schisandra chinensis, 4-9 parts of hovenia dulcis, and 20-25 parts of salvia miltiorrhiza.

2. The Chinese medicinal preparation for treating liver disease according to claim 1, characterized in that: Its preparation forms include injection, powder injection, lyophilized powder injection, tablets, capsules, granules, oral solution, pills or sustained-release preparations.

3. A method for preparing a Chinese medicine preparation for treating liver disease, applied to the Chinese medicine preparation for treating liver disease as claimed in any one of claims 1-2, characterized in that: The pretreatment of Pueraria lobata particles includes the following specific steps: S1: Weigh the kudzu root according to the formula, cut the kudzu root to be used into slices and dry them, add 500 ml of water to the dried kudzu root into a decoction pot, decoct for 1.5 hours, and repeat the decoction for 3 times until the kudzu root decoction in the pot is clear, filter the obtained medicinal liquid under negative pressure, concentrate it under reduced pressure at an ambient temperature of 50°C to a viscous state, place it in a drying oven for vacuum drying, and finally grind the obtained product to obtain kudzu root extract powder.

4. The method for preparing a Chinese medicine preparation for treating liver disease according to claim 3, characterized in that: Among them, the smilax glabra, yellow root and oxtail grass are uniformly pre-treated, including the following specific steps: Q1: Weigh smilax glabra, yellow root and oxtail grass according to the formula, mix them and add them into a treatment tank filled with an appropriate amount of water, extract them under slight boiling reflux at 48°C, and centrifuge the extract at 3000r / min for 5min. Filter the supernatant with a Buchner funnel, and dry it in a drying oven under vacuum. Finally, grind the product to obtain mixed extract powder A.

5. The method for preparing a Chinese medicine preparation for treating liver disease according to claim 4, characterized in that: The Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum are uniformly pre-treated, including the following specific steps: T1: According to the formula, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum were weighed and ground into coarse powder in a grinder. 80%-90% ethanol was added and immersed for 24 hours. After reflux extraction for 3 times, the obtained liquid was filtered under negative pressure, and concentrated under reduced pressure at an ambient temperature of 50°C to a viscous state. An appropriate amount of water was added, heated at 30-60°C, stirred and dissolved, and the ethanol was recovered, concentrated and dried to obtain mixed extract powder B.

6. The method for preparing a Chinese medicine preparation for treating liver disease according to claim 5, characterized in that: Wherein the Pueraria solution pretreatment comprises the following specific steps: S2: Weigh Pueraria root according to the formula, add the corresponding amount of water for reflux extraction, centrifuge the extract for 10 minutes, filter the supernatant with a Buchner funnel, collect and filter through a 0.3um microporous filter membrane, discard the initial filtrate to obtain the Pueraria root solution.

7. The method for preparing a Chinese medicine preparation for treating liver disease according to claim 6, characterized in that: Among them, the Chinese smilax glabra, yellow root and oxtail grass are pretreated with a unified solution, including the following specific steps: Q2: Weigh Smilax glabra, Rhizoma Coptidis and Herba Cynoglossi according to the formula, add corresponding times of water for reflux extraction, centrifuge the extract for 10 minutes, filter the supernatant with a Buchner funnel, collect and filter through a 0.3um microporous filter membrane, discard the initial filtrate to obtain mixed solution A.

8. The method for preparing a Chinese medicine preparation for treating liver disease according to claim 7, characterized in that: The Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum are uniformly pretreated with the solution, including the following specific steps: T2: Cordyceps militaris, Schisandra chinensis, Hovenia dulcis, Salvia miltiorrhiza and Ganoderma lucidum were weighed according to the formula, and the corresponding amount of water was added for reflux extraction. The extract was centrifuged for 10 minutes, and the supernatant was filtered with a Buchner funnel. After collection, the supernatant was filtered through a 0.3um microporous membrane, and the initial filtrate was discarded to obtain a mixed solution B.

9. The method for preparing a Chinese medicinal preparation for treating liver disease according to claim 8, characterized in that: The preparation of the powder injection, freeze-dried powder injection, tablet, capsule and granule of the Chinese medicine comprises the following specific steps: taking the kudzu root extract powder, mixed extract powder A and mixed extract powder B obtained from S1, Q1 and T1 and fully mixing them, further heating and drying them at 63°C, adding cross-linked starch at a mass ratio of 1:1, using an appropriate amount of 75°C ethanol to make a soft material, reducing the pressure at 60°C for 24 hours, filtering and sieving with a sieve to obtain Chinese medicine preparation particles, and further treating them to obtain freeze-dried powder injection, tablets, capsules and granules; The preparation of injection, oral liquid, pill and sustained-release preparation includes the following specific steps: taking the kudzu root solution, mixed solution A and mixed solution B obtained from S2, Q2 and T2, mixing them, stirring while heating until uniform, cooling to room temperature, and further processing to obtain injection, oral liquid, pill and sustained-release preparation.

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