Hermetia illucens sand organic fertilizer rich in indole-3-acetic acid and preparation method thereof
By using Lactobacillus plantarum and Chlorella nucleus in the process of treating kitchen waste by black soldier flies, the problems of low efficiency of insect sand and insufficient nutrients are solved, and efficient kitchen waste resource treatment and insect sand products are achieved. high-quality production.
Patent Information
- Application Number
- CN202411603512.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-02-18
AI Technical Summary
Among the existing methods of black soldier flies to treat kitchen waste, insect sand has low fertilizer effect and insufficient phytonutrient content, resulting in unstable fertilizer efficiency or seedling burning.
During the treatment of black soldier fly, add Lactobacillus plantarum CCTCCM2021576 and Chlorella nucleus liquor to the kitchen waste, promote the thorough decomposition and utilization of kitchen waste by black soldier fly larvae, and improve nitrogen, phosphorus and potassium in insect sand. The saving content of the elements and produces indole-3-acetic acid in insect sand.
It significantly improves the fertilizer effect of worm sand, increases the content of nitrogen, phosphorus and potassium, and produces indole-3-acetic acid in worm sand, improving the plant nutritional effect and economic value of worm sand.
Smart Images
Figure SMS_1 
Figure HDA0005128935440000011 
Figure HDA0005128935440000012
Abstract
Description
Technical Field
[0001] The invention relates to a technology for resource processing of environmental solid waste, and in particular to a black soldier fly sand organic fertilizer rich in indole-3-acetic acid and a preparation method thereof. Background Art
[0002] Kitchen waste is a type of solid waste with a large output and serious pollution in urban life. If not handled properly, it will cause varying degrees of pollution and damage to the soil, water sources, and the atmosphere. Using black soldier flies to treat kitchen waste is a new type of solid waste resource utilization method that breaks through traditional composting and anaerobic biogas fermentation. After kitchen waste is transformed by black soldier flies, black soldier fly larvae can be obtained, which can be used as animal feed. Residues containing insect feces can also be obtained, which can be used as soil fertilizer, thereby realizing the conversion of kitchen waste into feed and fertilizer.
[0003] The residue obtained after black soldier flies process food waste is usually called insect sand, which is an organic fertilizer containing nitrogen, phosphorus and potassium. However, food waste insect sand often has problems such as insufficient fertilizer efficiency, instability or burning seedlings, which are mainly caused by incomplete degradation of food waste and insufficient content of plant nutrients. Summary of the invention
[0004] The purpose of the present invention is to solve the problems of low fertilizer effect of insect sand and insufficient content of plant nutrients in the existing method for treating kitchen waste with black soldier flies, and to propose a method for preparing black soldier fly insect sand organic fertilizer with good fertilizer effect and rich in indole-3-acetic acid. The method uses kitchen solid waste as a substrate for black soldier flies, uses plant lactobacillus CCTCCM2021576 as an inoculated microorganism, and uses protein nucleus chlorella as an inoculated algae. By inoculating bacterial liquid twice in the early and middle stages of the treatment, and supplementing protein nucleus chlorella algae liquid, the degradation of kitchen waste is assisted and protein nutrition is provided to black soldier flies, the growth and development of black soldier flies larvae is promoted, the content of nitrogen, phosphorus and potassium elements in the insect sand product is increased, and indole-3-acetic acid is produced in the insect sand. The obtained insect sand product is rich in nitrogen, phosphorus, potassium elements and indole-3-acetic acid, wherein the indole-3-acetic acid content reaches 20-110 mg / kg, which provides an effective method for improving the fertilizer effect quality and economic value of black soldier flies insect sand.
[0005] It should be noted that, in the present invention, unless otherwise specified, the specific meaning of "including" in relation to composition limitations and descriptions includes both open-ended "including", "comprising", etc. and similar meanings, as well as closed-ended "composed of", "composed of", etc. and similar meanings.
[0006] To achieve the above object, the technical solution adopted by the present invention is: a method for preparing black soldier fly sand organic fertilizer rich in indole-3-acetic acid, comprising the following steps:
[0007] Step 1: Put the food waste into the black soldier fly treatment box, add wheat bran and stir evenly, then add chlorella algae liquid and Lactobacillus plantarum liquid and stir evenly, and finally add black soldier fly larvae to the food waste;
[0008] Step 2: Cultivate black soldier fly larvae at room temperature and stir once a day;
[0009] Step 3: On the 5th to 9th day of the culture, add the Chlorella pyrenoidosa liquid and the Lactobacillus plantarum liquid to the black soldier fly treatment box for a second time and stir evenly;
[0010] Step 4: On the 15th to 22nd day of breeding, the breeding is stopped, the black soldier fly larvae and the insect sand are separated by a sieve, and the insect sand is dried to obtain the insect sand organic fertilizer containing indole-3-acetic acid.
[0011] Furthermore, the food waste is food waste after cooking.
[0012] Furthermore, the weight of the wheat bran added in step 1 is 5-20% of the weight of the kitchen waste, preferably 10-15%.
[0013] Furthermore, the pyrenoid chlorella algae solution in step 1 is added in an amount of 30-100 ml / kg of food waste; preferably 40-60 ml / kg of food waste; the concentration of the pyrenoid chlorella algae solution is not less than 1×10 8 Pieces / liter.
[0014] Furthermore, the amount of the Lactobacillus plantarum liquid added in step 1 is 30-100 ml / kg of food waste; preferably 40-60 ml / kg of food waste; the concentration of Lactobacillus plantarum in the liquid is not less than 1×10 8 The Lactobacillus plantarum is Lactobacillus plantarum CCTCCM2021576, which is deposited in the China Center for Type Culture Collection, the name of the deposited culture is Lactobacillus plantarum RS72, the deposit number is CCTCC No.M2021576, and the deposit date is May 24, 2021.
[0015] Furthermore, the size of the black soldier fly larvae in step 1 is 0.01-0.04 g / larvae, preferably 0.02-0.04 g / larvae; the amount of black soldier fly larvae added is 1000-5000 / kg of food waste, preferably 2000-3000 / kg of food waste.
[0016] Furthermore, the room temperature in step 2 is 20-35°C, preferably 25-32°C.
[0017] Further, in step 3, 30-100 ml of pyrenoid Chlorella algae solution is added to each kilogram of food waste; preferably, 40-60 ml of pyrenoid Chlorella algae solution is added to each kilogram of food waste; the concentration of the pyrenoid Chlorella algae solution is not less than 1×10 8 Pieces / liter.
[0018] Further, in step 3, on the 5th to 9th day of cultivation, 30-100 ml of Lactobacillus plantarum liquid is added to each kilogram of food waste, and preferably, on the 6th to 8th day of cultivation, 40-60 ml of Lactobacillus plantarum liquid is added to each kilogram of food waste; the concentration of Lactobacillus plantarum in the liquid is not less than 1×10 8 The concentration of Lactobacillus plantarum in the bacterial solution is not less than 1×10 8 The Lactobacillus plantarum is Lactobacillus plantarum CCTCCM2021576, which is deposited in the China Center for Type Culture Collection, the name of the deposited culture is Lactobacillus plantarum RS72, the deposit number is CCTCCNo.M2021576, and the deposit date is May 24, 2021.
[0019] Furthermore, in step 4, the culture is stopped on the 16th to 21st day of culture.
[0020] Furthermore, in step 4, the insect sand is dried in a ventilated place for 2-4 days, preferably 3-4 days.
[0021] The pyrenoid chlorella used in the present invention has the ability to synthesize proteins using small molecules, and the plant lactobacillus has the ability to decompose tryptophan to generate indole-3-acetic acid. The present invention promotes the thorough digestion and degradation of food waste by black soldier fly larvae by adding pyrenoid chlorella and plant lactobacillus in the treatment of food waste, improves the storage capacity of insect sand for nitrogen, phosphorus and potassium elements, and produces indole-3-acetic acid substances in the insect sand. Indole-3-acetic acid is a plant growth hormone, which can be produced by plants themselves, or produced by microorganisms decomposing tryptophan, or synthesized by the chemical industry. The present invention strengthens the retention of nitrogen, phosphorus and potassium elements in the food waste treatment system by combining pyrenoid chlorella and plant lactobacillus, and promotes the generation and conversion of indole-3-acetic acid, and finally produces an insect sand organic fertilizer product rich in indole-3-acetic acid with a significant fertilizer effect, providing a solution for the stability and high quality of the fertilizer effect of insect sand.
[0022] Another object of the present invention is to disclose a black soldier fly sand organic fertilizer rich in indole-3-acetic acid, which is prepared by the above method.
[0023] Furthermore, in the black soldier fly sand organic fertilizer rich in indole-3-acetic acid, the content of each nutrient based on the dry weight of the sand is as follows: indole-3-acetic acid content is 20-110 mg / kg, nitrogen content is 22-35 mg / g, phosphorus content is 10-25 mg / g, and potassium content is 25-45 mg / g.
[0024] Another object of the present invention is to disclose a method for preparing a tea bag type black soldier fly sand organic fertilizer rich in indole-3-acetic acid, comprising the following steps:
[0025] The insect sand organic fertilizer containing indole-3-acetic acid is crushed and stirred in a blender and packed into transparent nylon bags of tea bag size, 0.5-2g per bag, for plant cultivation.
[0026] Another object of the present invention is to disclose a tea bag type organic fertilizer containing black soldier fly sand and rich in indole-3-acetic acid, which is prepared by the above method. The obtained sand is dried and crushed, and then put into a tea bag type nylon cloth bag, which can be used as an organic fertilizer for plants.
[0027] The black soldier fly sand organic fertilizer rich in indole-3-acetic acid and the preparation method thereof have the following advantages compared with the prior art:
[0028] 1) The present invention promotes the thorough decomposition and utilization of kitchen waste by black soldier fly larvae by adding protein core chlorella algae liquid and plant lactobacillus liquid to kitchen waste at the same time, and at the same time increases the storage content of nitrogen, phosphorus and potassium elements in insect sand. The nutrient element content of the insect sand organic fertilizer is as follows: nitrogen content is 22-35 mg / g, phosphorus content is 10-25 mg / g, and potassium content is 25-45 mg / g, which exceeds the national standard NY252-2012 for organic fertilizers, that is, the total content of nitrogen, phosphorus and potassium elements is higher than 50 mg / g. Since the macromolecular organic matter is fully degraded and the nitrogen, phosphorus and potassium elements are rich, this organic fertilizer does not have the phenomenon of burning seedlings or low fertilizer efficiency, and has good plant nutrition.
[0029] 2) The present invention adopts a plant lactobacillus CCTCCM2021576, which can convert tryptophan into organic substances such as indole-3-acetic acid and indole during growth and metabolism, as shown in the following formula.
[0030]
[0031] 3) The present invention adds plant lactobacillus CCTCCM2021576 and pyrenoid chlorella to the kitchen waste, which can not only promote the growth and development of black soldier fly larvae, but also further enhance the generation and yield of indole-3-acetic acid in the sand of black soldier flies. Among them, plant lactobacillus CCTCCM2021576 can promote the metabolism of carbohydrates in kitchen waste, and hydrolyze protein to convert tryptophan into indole-3-acetic acid. Pyrenoid chlorella has the ability to reproduce by utilizing ammonia nitrogen and phosphate, and can also serve as a protein source for black soldier flies, promoting the digestion of protein by black soldier flies, and digesting and metabolizing tryptophan into indole-3-acetic acid.
[0032] 4) The obtained insect sand is dried and crushed, then packed into tea bag-type nylon cloth bags and can be used as organic fertilizer for plants.
[0033] In summary, the method for preparing the black soldier fly sand organic fertilizer rich in indole-3-acetic acid of the present invention increases the decomposition efficiency of kitchen waste, improves the storage content of nitrogen, phosphorus and potassium elements in the sand, and promotes the decomposition and metabolism of tryptophan by black soldier flies and their microorganisms, thereby producing sand rich in indole-3-acetic acid, which helps to improve the fertilizer quality and economic value of black soldier fly sand. BRIEF DESCRIPTION OF THE DRAWINGS
[0034] Figure 1 The figures are the liquid chromatography test results of the standard sample, the embodiment and the comparative example; wherein A is the high performance liquid chromatography test spectrum of the standard samples of indole-3-acetic acid, p-cresol, indole and indole-3-methyl; B is the high performance liquid chromatography test spectrum of the insect sand to which Lactobacillus plantarum and Chlorella pyrenoidosa are added (Example 3); and C is the high performance liquid chromatography test spectrum of the insect sand to which Lactobacillus plantarum and Chlorella pyrenoidosa are not added (Comparative Example 1).
[0035] Figure 2 It is the actual product, usage scenarios and effects of the tea bag-type black soldier fly sand organic fertilizer rich in indole-3-acetic acid. DETAILED DESCRIPTION
[0036] The present invention is further described below with reference to the examples. The description of the technical features described below is based on representative embodiments and specific examples of the present invention, but the present invention is not limited to these embodiments and specific examples. It should be noted that:
[0037] Unless otherwise stated, the units used in this specification are all international standard units, and the numerical values and numerical ranges appearing in the present invention should be understood to include the inevitable systematic errors in industrial production.
[0038] In this specification, the numerical range expressed using "a numerical value A to a numerical value B" means a range including the endpoints numerical values A and B.
[0039] In the present specification, a numerical range expressed using "above" or "below" means a numerical range including the number.
[0040] In this specification, the word "may" means both performing a certain process and not performing a certain process.
[0041] In this specification, the use of "optional" or "optional" indicates that certain substances, components, execution steps, application conditions and other factors are used or not used.
[0042] In this specification, when "normal temperature" or "room temperature" is used, the temperature may be 15-25°C.
[0043] In this manual, the reagents or instruments used without indicating the manufacturer are all conventional products that can be obtained through commercial purchase.
[0044] Example 1
[0045] This embodiment discloses a tea bag type black soldier fly sand organic fertilizer rich in indole-3-acetic acid and a preparation method thereof, which is implemented by the following steps:
[0046] Step 1: Put the food waste into the black soldier fly treatment box, add wheat bran according to 5% of the weight of the food waste, stir evenly, and continue to add 30 ml of 5.2×10 8 / L of pyrenoid Chlorella algae liquid, and 30 ml of 3.1×10 8 1000 black soldier fly larvae with a size of 0.01-0.03 g were added to each kilogram of food waste after stirring evenly.
[0047] Step 2: Cultivate black soldier fly larvae at a room temperature of 20° C. and stir once a day;
[0048] Step 3: On the fifth day of culture, add 30 ml of 1.8×10 8 / L of Chlorella pyrenoidosa liquid, and 30 ml of 2.2×10 8 100ml / L of Lactobacillus plantarum CCTCCM2021576 bacterial solution, stir evenly;
[0049] Step 4: On the 15th day of culture, the culture was stopped, the black soldier fly larvae and the insect sand were separated by a sieve, and the insect sand was dried in a ventilated place for 2 days;
[0050] Step 5: Recover the insect sand obtained in step 4 to obtain an indole-containing insect sand organic fertilizer, crush and stir the insect sand in a blender, and put it into a transparent nylon bag of the size of a tea bag to obtain a tea bag-sized black soldier fly insect sand organic fertilizer rich in indole-3-acetic acid that can be used for plant cultivation.
[0051] After testing, the nutrient content of black soldier fly sand based on the dry weight of the sand is: indole-3-acetic acid 27.8 mg / kg, nitrogen 25.1 mg / g, phosphorus 14.2 mg / g, and potassium 27.7 mg / g.
[0052] Example 2
[0053] This embodiment discloses a tea bag type black soldier fly sand organic fertilizer rich in indole-3-acetic acid and a preparation method thereof, which is implemented by the following steps:
[0054] Step 1: Put the food waste into the black soldier fly treatment box, add wheat bran according to 10% of the weight of the food waste, stir evenly, and continue to add 50 ml of 5.2×10 8 / L of Chlorella pyrenoidosa liquid, and 50 ml of 3.1×10 8 100 g / L of plant lactobacillus CCTCCM2021576 bacterial solution was stirred evenly, and then 3000 black soldier fly larvae with a size of 0.01-0.02 g were added to each kilogram of food waste;
[0055] Step 2: Cultivate black soldier fly larvae at a room temperature of 27° C. and stir once a day;
[0056] Step 3: On the 7th day of culture, add 50 ml of 1.8×10 8 50 ml of 2.2×10 8 100ml / L of Lactobacillus plantarum CCTCCM2021576 bacterial solution, stir evenly;
[0057] Step 4: On the 17th day of culture, the culture was stopped, the black soldier fly larvae and the insect sand were separated by a sieve, and the insect sand was dried in a ventilated place for 3 days;
[0058] Step 5: Recover the insect sand obtained in step 4 to obtain an indole-containing insect sand organic fertilizer, crush and stir the insect sand in a blender, and put it into a transparent nylon bag of the size of a tea bag to obtain a tea bag-sized black soldier fly insect sand organic fertilizer rich in indole-3-acetic acid that can be used for plant cultivation.
[0059] After testing, the content of various nutrients in black soldier fly sand based on the dry weight of the sand is: indole-3-acetic acid 55.1 mg / kg, nitrogen 28.9 mg / g, phosphorus 16.7 mg / g, and potassium 31.8 mg / g.
[0060] Example 3
[0061] This embodiment discloses a tea bag type black soldier fly sand organic fertilizer rich in indole-3-acetic acid and a preparation method thereof, which is implemented by the following steps:
[0062] Step 1: Put the food waste into the black soldier fly treatment box, add wheat bran according to 20% of the weight of the food waste, stir evenly, and continue to add 100 ml of 5.2×10 8 100 ml of 3.1×10 8 100 g / L of plant lactobacillus CCTCCM2021576 bacterial solution was stirred evenly, and then 5,000 black soldier fly larvae with a size of 0.02-0.04 g were added to each kilogram of food waste;
[0063] Step 2: Cultivate black soldier fly larvae at a room temperature of 35° C. and stir once a day;
[0064] Step 3: On the 9th day of culture, add 100 ml of 1.8×10 8 100 ml of 2.2×10 8 100ml / L of Lactobacillus plantarum CCTCCM2021576 bacterial solution, stir evenly;
[0065] Step 4: On the 22nd day of culture, the culture was stopped, the black soldier fly larvae and the insect sand were separated by a sieve, and the insect sand was dried in a ventilated place for 4 days;
[0066] Step 5: Recover the insect sand obtained in step 4 to obtain an insect sand organic fertilizer containing indole-3-acetic acid, crush and stir the insect sand in a blender, and put it into a transparent nylon bag of the size of a tea bag to obtain a tea bag-sized black soldier fly insect sand organic fertilizer rich in indole-3-acetic acid that can be used for plant cultivation.
[0067] After testing, the content of various nutrients in black soldier fly sand based on the dry weight of the sand is: indole-3-acetic acid 102.9 mg / kg, nitrogen 32.6 mg / g, phosphorus 18.1 mg / g, potassium 40.2 mg / g.
[0068] Comparative Example 1
[0069] This comparative example discloses a tea bag type black soldier fly sand organic fertilizer and a preparation method thereof, which is implemented by the following steps:
[0070] Step 1: Put the food waste into a black soldier fly treatment box, add wheat bran according to 20% of the weight of the food waste, stir evenly, and add 5,000 black soldier fly larvae with a size of 0.02-0.04g per kilogram of food waste;
[0071] Step 2: Cultivate black soldier fly larvae at a room temperature of 35° C. and stir once a day;
[0072] Step 3: On the 22nd day of culture, the culture is stopped, the black soldier fly larvae and the insect sand are separated by a sieve, the insect sand is dried in a ventilated place for 4 days, and the black soldier fly insect sand is recovered.
[0073] After testing, the content of various nutrients in black soldier fly sand based on the dry weight of the sand is: indole-3-acetic acid 0 mg / kg, nitrogen 23.3 mg / g, phosphorus 12.1 mg / g, and potassium 10.1 mg / g.
[0074] Figure 1 The figures are the liquid chromatography test results of the standard sample, the embodiment and the comparative example; wherein A is the high performance liquid chromatography test spectrum of the standard samples of indole-3-acetic acid, p-cresol, indole and indole-3-methyl; B is the high performance liquid chromatography test spectrum of the insect sand sample obtained in Example 3 of the present invention (using Lactobacillus plantarum and Chlorella pyrenoidosa additives); and C is the high performance liquid chromatography test spectrum of the insect sand sample obtained in Comparative Example 1 of the present invention (not using Lactobacillus plantarum and Chlorella pyrenoidosa additives).
[0075] Figure 2 The actual product and usage scenario of the tea bag-style black soldier fly sand organic fertilizer rich in indole-3-acetic acid.
[0076] By comparing Example 3 with Comparative Example 1, it can be seen that the method of the present invention provides a method for producing black soldier fly sand organic fertilizer rich in indole-3-acetic acid by strengthening Lactobacillus plantarum and Chlorella pyrenoidosa, which provides a new idea for promoting the resource utilization of black soldier flies to treat solid waste and improve the value and quality of products.
[0077] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.
Claims
1. A method for preparing black soldier fly sand organic fertilizer rich in indole-3-acetic acid, characterized in that: The following steps are involved: Step 1: Put the food waste into the black soldier fly treatment box, add wheat bran and stir evenly, then add chlorella algae liquid and Lactobacillus plantarum liquid and stir evenly, and finally add black soldier fly larvae to the food waste; Step 2: Cultivate black soldier fly larvae at room temperature and stir once a day; Step 3: On the 5th to 9th day of the culture, add the Chlorella pyrenoidosa liquid and the Lactobacillus plantarum liquid to the black soldier fly treatment box for a second time and stir evenly; Step 4: On the 15th to 22nd day of breeding, the breeding is stopped, the black soldier fly larvae and the insect sand are separated by a sieve, and the insect sand is dried to obtain the insect sand organic fertilizer containing indole-3-acetic acid.
2. The method for preparing the black soldier fly sand organic fertilizer rich in indole-3-acetic acid according to claim 1, characterized in that: The weight of the wheat bran added in step 1 is 5-20% of the weight of the kitchen waste; And / or, the amount of the pyrenoid chlorella algae liquid added in step 1 is 30-100 ml / kg of food waste; the concentration of the pyrenoid chlorella algae liquid is not less than 1×10 8 Pieces / liter; And / or, the amount of the bacterial solution added in step 1 is 30-100 ml / kg of food waste; the plant lactobacillus is plant lactobacillus CCTCCM2021576; the concentration of plant lactobacillus in the bacterial solution is not less than 1×10 8 Pieces / liter; And / or, the size of the black soldier fly larvae in step 1 is 0.01-0.04 g / larvae; the amount of black soldier fly larvae added is 1000-5000 / kg of food waste.
3. The method for preparing the black soldier fly sand organic fertilizer rich in indole-3-acetic acid according to claim 1, characterized in that: The room temperature in step 2 is 20-35°C.
4. The method for preparing the black soldier fly sand organic fertilizer rich in indole-3-acetic acid according to claim 1, characterized in that: In step 3, the amount of the Chlorella pyrenoidosa liquid added is 30-100 ml / kg of kitchen waste; the concentration of the Chlorella pyrenoidosa liquid is not less than 1×10 8 Pieces / liter; And / or, the amount of the plant lactobacillus liquid added in step 3 is 30-100 ml / kg of food waste; the plant lactobacillus is plant lactobacillus CCTCCM2021576, and the concentration of plant lactobacillus in the liquid is not less than 1×10 8 Pieces / liter.
5. The method for preparing the black soldier fly sand organic fertilizer rich in indole-3-acetic acid according to claim 1, characterized in that: Step 4: Stop the culture on the 16th to 21st day of culture; And / or, in step 4, the insect sand is dried in a ventilated place for 2-4 days.
6. A black soldier fly sand organic fertilizer rich in indole-3-acetic acid, characterized in that: The method is prepared by any one of claims 1 to 5.
7. The black soldier fly sand organic fertilizer rich in indole-3-acetic acid according to claim 6, characterized in that: The black soldier fly sand organic fertilizer rich in indole-3-acetic acid has an indole-3-acetic acid content of 20-110 mg / kg, a nitrogen content of 22-35 mg / g, a phosphorus content of 10-25 mg / g, and a potassium content of 25-45 mg / g.
8. A method for preparing a tea bag type black soldier fly sand organic fertilizer rich in indole-3-acetic acid, characterized in that: The following steps are involved: The insect sand organic fertilizer containing indole-3-acetic acid as claimed in claim 6 or 7 is crushed and stirred in a blender, and put into transparent nylon bags of tea bag size, 0.5-2g per bag, to obtain tea bag-sized black soldier fly insect sand organic fertilizer rich in indole-3-acetic acid that can be used for plant cultivation.
9. A tea bag type black soldier fly sand organic fertilizer rich in indole-3-acetic acid, characterized in that The method according to claim 8 is used for preparation.