Preparation method and application of wax gourd biomass hydrogel film

Abstract

The invention discloses a preparation method and application of a white gourd biomass hydrogel film. The preparation method comprises the following steps: building a biomass hydrogel film by using a natural three-dimensional porous structure of white gourd, obtaining white gourd aerogel by a mild dehydration technology, and introducing macromolecular hydrogel to obtain the white gourd biomass hydrogel film. The hydrogel film is simple to form, strong in mechanical property and good in biodegradability, can be applied to preparation of tissue engineering scaffolds, postoperative adhesion prevention mucous membranes, drug-loaded pasting films, facial masks and the like, is high in added value of products and is suitable for large-scale popularization and application.

Description

Technical Field

[0001] The invention belongs to the processing technology of biomass film materials, and specifically relates to a preparation method and application of wax gourd biomass hydrogel film. Background Art

[0002] Biomass membrane technology is a technology for preparing membranes using natural biomass materials. These membranes have good biocompatibility, degradability and environmental friendliness, and are widely used in wastewater treatment, air purification, medical health and food packaging. The preparation methods of biomass membranes mainly include solution casting, phase inversion, electrostatic spinning, etc. Commonly used biomass materials include cellulose, chitosan, sodium alginate, gelatin, etc. These materials have good film-forming properties and functional properties, such as antibacterial properties, adsorption and biodegradability. CN202311493993.6 discloses a biomass-based nanofiltration membrane, a preparation method and an application. The main materials of nanocellulose and nanochitin are prepared by vacuum filtration or natural drying combined with chemical cross-linking to obtain a biomass-based nanofiltration membrane, which can be widely used in organic solvent filtration, drug filtration, virus filtration and wastewater treatment. CN202311601790.4 discloses a high-toughness biomass composite membrane and its preparation method, which is made of konjac powder and dialdehyde starch as raw materials to make a solution and then dried, and can be applied to the field of food packaging. CN202210924666.0 discloses a high-strength biomass material and its preparation method and biomass film, which are composed of modified plant fiber, modified gelatin, and modified calcium lactate, and have excellent tensile properties and ductility, and can also have good heat resistance, high moisture permeability and good hydrophobicity. The above-mentioned biomass membranes are all solidified and formed by biological macromolecular solutions, and the preparation efficiency is low. There are still many defects in the structure and performance of the membrane. Directly using the structure evolved from nature to prepare biomass membranes can achieve twice the result with half the effort. Among them, the wax gourd has a large body, a network porous structure inside, and is cut into a sheet-like form, which can further prepare biomass membranes, and can also be given special functions to the membrane material as needed, greatly simplifying the preparation process of biomass membranes, and also expanding the application field of wax gourd. Therefore, the technology of the present invention provides innovative ideas and methods for developing biomass membranes. Summary of the invention

[0003] The present invention aims to develop a biomass hydrogel membrane using wax gourd, give full play to the three-dimensional network structure of the wax gourd hydrogel, and construct a hydrogel membrane with good permeability and strong mechanical properties. The prepared membrane can be used to prepare high-value materials such as tissue engineering scaffolds, anti-postoperative mucosal membranes, drug-loaded patches, and facial masks. In order to achieve this purpose, the preparation method provided by the present invention comprises the following steps: S1. Cut the wax gourd into continuous slices of equal thickness along the circumference, and wash the wax gourd slices in a baking soda solution; S2. The washed wax gourd slices were immersed in a heated polyethylene glycol solution for dehydration, then immersed in an ethanol solution for washing, and vacuum dried to obtain a wax gourd aerogel film; S3. Immerse the wax gourd aerogel film in a polymer solution, or then transfer it to an ethanol or glycerol solution to obtain a wax gourd biomass hydrogel film, wherein the polymer is one or more of chitosan, sodium alginate, gelatin, silk fibroin, polyvinyl alcohol, hyaluronic acid, and polyglutamic acid.

[0004] Furthermore, the thickness of the wax gourd slices in S1 is 0.5-3 mm, and the mass concentration of baking soda is 0.05%-0.5%.

[0005] Furthermore, the molecular weight of the polyethylene glycol in S2 is 4000-20000, the heating temperature is 50-80° C., and the concentration of the ethanol solution is 75%-100%.

[0006] Furthermore, the mass concentration of the polymer solution in S3 is 0.5% to 10%, and the polymer is one or more of chitosan, sodium alginate, gelatin, silk fibroin, polyvinyl alcohol, hyaluronic acid, and polyglutamic acid.

[0007] Furthermore, the ethanol or glycerol solution in S3 contains a small amount of metal salt, and the metal salt is one of zinc sulfate, silver nitrate, copper sulfate, and calcium chloride, and its mass concentration is 0.01%~1%.

[0008] In addition, the present invention provides a wax gourd biomass hydrogel film obtained by the above preparation method.

[0009] Furthermore, the present invention also provides the use of wax gourd biomass hydrogel film in the preparation of tissue engineering scaffolds, anti-postoperative mucosal membranes, drug-loaded patches, and facial masks. Beneficial Effects

[0010] (1) Using the natural three-dimensional porous structure of wax gourd to build biomass hydrogel membrane, the hydrogel membrane has good formability, strong mechanical properties, good biodegradability, strong product application expansion, and great potential market demand; (2) The technical method of the present invention has simple process and mild conditions, and can be used to produce wax gourd biomass hydrogel film on a large scale, thereby increasing the added value of the product, meeting the development requirements of green, low-carbon and environmental protection, and being suitable for large-scale promotion and application. DETAILED DESCRIPTION

[0011] The present invention is further described below in conjunction with specific embodiments, but is not intended to limit the present invention. Example

[0012] The wax gourd was peeled and the pulp removed, and sliced ​​into 1 mm thick slices along the circumference. The wax gourd slices were then immersed in a 0.05% baking soda solution for cleaning. The washed wax gourd slices were immersed in a 50°C polyethylene glycol 4000 solution for dehydration, and then immersed in 100% ethanol for cleaning, followed by vacuum drying to obtain wax gourd aerogel. The obtained wax gourd aerogel was immersed in a 0.5% chitosan solution, and then transferred to an ethanol solution containing 1% calcium chloride to obtain a wax gourd biomass hydrogel film. Example

[0013] The wax gourd was peeled and the pulp removed, and sliced ​​into 3 mm thick slices along the circumference. The wax gourd slices were then immersed in a 0.5% baking soda solution for cleaning. The washed wax gourd slices were immersed in a polyethylene glycol 20000 solution at 80°C for dehydration, and then immersed in 75% ethanol for cleaning, followed by vacuum drying to obtain wax gourd aerogel. The obtained wax gourd aerogel was immersed in a 10% gelatin solution, and then transferred to a glycerol solution containing 0.01% silver nitrate to obtain a wax gourd biomass hydrogel film. Example

[0014] The wax gourd was peeled and the pulp removed, and sliced ​​into 2 mm thick slices along the circumference. The wax gourd slices were then immersed in a 0.2% baking soda solution for cleaning. The washed wax gourd slices were immersed in a 60°C polyethylene glycol 8000 solution for dehydration, then immersed in 85% ethanol for cleaning, and then vacuum dried to obtain wax gourd aerogel. The obtained wax gourd aerogel was immersed in a 5% polyvinyl alcohol solution, and then transferred to an ethanol solution containing 0.2% zinc sulfate to obtain a wax gourd biomass hydrogel film. Example

[0015] The wax gourd was peeled and the pulp removed, and sliced ​​into 1 mm thick slices along the circumference. The wax gourd slices were then immersed in a 0.05% baking soda solution for cleaning. The washed wax gourd slices were immersed in a 50°C polyethylene glycol 6000 solution for dehydration, then immersed in 90% ethanol for cleaning, and then vacuum dried to obtain wax gourd aerogel. The obtained wax gourd aerogel was immersed in a 2% hyaluronic acid solution, and then transferred to a glycerol solution containing 0.05% copper sulfate to obtain a wax gourd biomass hydrogel film.

Claims

1. A method for preparing a wax gourd biomass hydrogel film, characterized in that: Using wax gourd as the substrate and introducing other polymers to form a composite hydrogel film specifically includes the following steps: S1. Cut the wax gourd into continuous slices of equal thickness along the circumference, and wash the wax gourd slices in a baking soda solution; S2. The washed wax gourd slices were immersed in a heated polyethylene glycol solution for dehydration, then immersed in an ethanol solution for washing, and vacuum dried to obtain a wax gourd aerogel film; S3. Immerse the wax gourd aerogel film in a polymer solution, or then transfer it to an ethanol or glycerol solution to obtain a wax gourd biomass hydrogel film, wherein the polymer is one or more of chitosan, sodium alginate, gelatin, silk fibroin, polyvinyl alcohol, hyaluronic acid, and polyglutamic acid.

2. The method for preparing a wax gourd biomass hydrogel film according to claim 1, characterized in that: The thickness of the wax gourd slices in S1 is 0.5~3 mm, and the mass concentration of baking soda is 0.05%~0.5%.

3. The method for preparing a wax gourd biomass hydrogel film according to claim 1, characterized in that: The molecular weight of the polyethylene glycol in S2 is 4000-20000, the heating temperature is 50-80° C., and the concentration of the ethanol solution is 75%-100%.

4. The method for preparing a wax gourd biomass hydrogel film according to claim 1, characterized in that: The mass concentration of the polymer solution in S3 is 0.5% to 10%, and the polymer is one or more of chitosan, sodium alginate, gelatin, silk fibroin, polyvinyl alcohol, hyaluronic acid, and polyglutamic acid.

5. The method for preparing a wax gourd biomass hydrogel film according to claim 1, characterized in that: The ethanol or glycerol solution in S3 contains a small amount of metal salt, and the metal salt is one of zinc sulfate, silver nitrate, copper sulfate, and calcium chloride, and its mass concentration is 0.01%~1%.

6. The wax gourd biomass hydrogel film obtained according to any one of the preparation methods described in claims 1 to 5.

7. The wax gourd biomass hydrogel film according to claim 6 is used to prepare tissue engineering scaffolds, anti-postoperative mucosal membranes, drug-loaded patches, and facial masks.