Rinsing device and rinsing method for tissue slice antibody after frozen section

Abstract

The invention provides a rinsing device and a rinsing method for a tissue slice antibody after a frozen slice, and belongs to the field of biomedicine. Comprising a rinsing liquid box and a screen receiving plate, a plurality of placing holes are formed in the screen receiving plate, tissue screens are arranged in the placing holes, and a first driving assembly used for driving rinsing liquid is arranged on the rinsing liquid box. The height of a screen receiving plate is adjusted through a lifting assembly, a plurality of tissue screens are lowered into a rinsing liquid box, a plurality of rotating shafts and fan blades are located in gaps, then a first motor is started to drive one of the rotating shafts to rotate, the rotating shafts are driven to rotate at the same time under the action of a belt wheel and a transmission belt, and the multiple tissue screens are driven to rotate at the same time. The plurality of rotating shafts drive the circular block and the fan blades to rotate, so that the rinsing liquid in the rinsing liquid box flows from one end to the other end through the gap, and the rinsing liquid is circulated in sequence, so that the rinsing effect of the rinsing liquid on the tissue slices in the tissue screen is enhanced.

Description

Technical Field

[0001] The invention relates to the field of biomedicine, and more particularly to a rinsing device and a rinsing method for antibodies in tissue sections after frozen sections. Background Art

[0002] Cryosectioning is a commonly used tissue sectioning technique, which uses low temperature to quickly freeze and harden the tissue, and then uses a slicer to cut it into thin slices. This technique can preserve the morphology and structure of the tissue while reducing the loss of antigens. Therefore, it is widely used in experiments such as immunohistochemistry and immunofluorescence. Its purpose is to remove unbound antibodies and impurities in the washing solution to reduce background staining and nonspecific coloring, and improve the accuracy and reliability of the experiment.

[0003] During use of the existing tissue slice antibody rinsing device after frozen sectioning, a proper amount of rinsing liquid is usually poured into the rinsing liquid tank, and then the tissue screen is placed in the screen receiving plate. The assembled screen receiving plate is adjusted to a suitable height through a lifting mechanism, so that the tissue slice floats in the rinsing liquid, and the rinsing liquid is kept in a flowing state through a circulation mechanism to rinse the tissue slice. However, during the rinsing process, the tissue slice is easily directly impacted by the rinsing liquid, which causes the integrity of the tissue slice to be easily destroyed during the rinsing. Summary of the invention

[0004] In view of the problem in the prior art that tissue sections are easily directly impacted by the rinsing liquid during the rinsing process, thereby easily destroying the integrity of the tissue sections during the rinsing, the purpose of the present invention is to provide a rinsing device and a rinsing method for tissue section antibodies after frozen sectioning.

[0005] To solve the above problems, the present invention adopts the following technical solutions:

[0006] A rinsing device and method for tissue section antibodies after frozen sectioning, comprising a rinsing liquid tank and a screen receiving plate, characterized in that a plurality of placement holes are provided on the screen receiving plate, and tissue screens are arranged in the plurality of placement holes, a first driving component for driving the rinsing liquid is provided on the rinsing liquid tank, a vibration component for vibrating the tissue screen is provided on the screen receiving plate, a lifting component for lifting the screen receiving plate is provided on the rinsing liquid tank, a mounting component for installing the screen receiving plate is provided on the lifting component, and a receiving component for receiving the rinsing liquid is provided on the rinsing liquid tank.

[0007] Optionally, the first driving assembly includes multiple rotating shafts, multiple rotating shafts all rotate and pass through one end of the rinsing liquid tank, a circular block is fixedly connected to one side of the rotating shaft, a plurality of fan blades are fixedly connected to the outer surface of the circular block, and gaps that cooperate with the plurality of fan blades are provided between the multiple tissue screens, a fixed plate is fixedly connected to one side of the rinsing liquid tank, and multiple rotating shafts are rotatably connected to the fixed plate on one side away from the rinsing liquid tank, a first L-shaped plate is fixedly connected to one side of the rinsing liquid tank, and a first motor is fixedly connected to the inner side of the first L-shaped plate, one of the rotating shafts is fixedly connected to the output end of the first motor, and the output end of the first motor rotates and passes through the fixed plate, and pulleys are fixedly connected to the outer surfaces of the multiple rotating shafts, and the same transmission belt is provided between the multiple pulleys.

[0008] Optionally, the bottom ends of the multiple tissue screens are symmetrically fixedly connected with semicircular blocks at the front and back, a connecting shaft is fixedly connected between two corresponding semicircular blocks, the outer surface of the connecting shaft is rotatably connected with a rectangular plate, and the bottom end of the tissue screen is fixedly connected with a blocking plate, and the blocking plate is located between the two rectangular plates.

[0009] Optionally, the vibration assembly includes a connecting frame, which is arranged on a screen receiving plate, the front end of the connecting frame is fixedly connected to a second L-shaped plate, a rotating shaft is rotatably connected to the rear end of the second L-shaped plate and the connecting frame, a half-shaped gear is fixedly connected to the outer surface of the rotating shaft, the front end of the connecting frame is slidably connected to a rack plate, the rack plate and the rotating shaft are meshed with each other, a knocking rod is symmetrically fixedly connected to the rack plate on the left and right, the front end of the connecting frame is symmetrically fixedly connected to a fixed block on the left and right, the outer surface of the rotating shaft is fixedly connected to a first circular gear, and the output end of the first motor is fixedly connected to a second circular gear.

[0010] Optionally, the lifting assembly includes two screws, both of which are rotatably connected to the top of the rinsing liquid tank, and the outer surfaces of the two screws are fixedly connected to worm gears. The rinsing liquid tank is provided with two connecting plates, and one side of one of the connecting plates is fixedly connected to a third L-shaped plate, the inner side of the third L-shaped plate is fixedly connected to a second motor, and the output end of the second motor is fixedly connected to a worm, and the worm rotatably passes through one of the connecting plates, and the worm cooperates with the two worm gears. Two limit rods are fixedly connected to the top of the rinsing liquid tank, and guide blocks are provided on the outer surfaces of the screws and the limit rods, two of which are threadedly connected to the screws, and the other two screws are slidably connected to the limit rods.

[0011] Optionally, the mounting assembly includes four rectangular blocks, the four rectangular blocks are respectively fixedly connected to four guide blocks, the connecting frame is fixedly connected to the two front rectangular blocks, the top ends of the four rectangular blocks are rotatably connected to connecting columns, the top ends of the connecting columns are fixedly connected to a rotating block, one side of the rotating block is fixedly connected to a first magnet, the side of the guide block close to the rectangular block is fixedly connected to a second magnet, and the four corners of the screen receiving plate are fixedly connected to a fourth L-shaped plate, and the fourth L-shaped plate is provided with a rectangular groove that cooperates with the rotating block.

[0012] Optionally, the receiving assembly includes two inclined plates, the top ends of the two inclined plates are fixedly connected to a peripheral edge, a drainage port is provided on the peripheral edge, and a second driving assembly is provided on the rinse liquid tank.

[0013] Optionally, the second driving assembly includes a base plate, which is fixedly connected to the bottom end of the rinse liquid tank, the connecting plate is fixedly connected to the top of the base plate, the top of the base plate is fixedly connected to four limit plates, one side of one of the limit plates is fixedly connected to a fifth L-shaped plate, the inner side of the fifth L-shaped plate is fixedly connected to a third motor, the output end of the third motor is fixedly connected to a bidirectional threaded rod, the bidirectional threaded rod rotates and passes through one of the limit plates, two movable plates are threadedly connected to the bidirectional threaded rod, the two inclined plates are respectively fixedly connected to the movable plates, a guide rod is fixedly connected between the two limit plates, and the two movable plates are both slidably connected to the guide rod.

[0014] Optionally, the inner bottom wall of the rinse liquid tank is provided with an inclined surface, a drainage hole is opened on one side of the rinse liquid tank, a rubber plug is engaged in the drainage hole, and a pull ring is fixedly connected to one side of the rubber plug.

[0015] Optionally, S1: adding an appropriate amount of rinsing solution into the rinsing solution box to ensure that sufficient rinsing solution can cover all tissue sections;

[0016] S2: placing the tissue slice into the tissue sieve for carrying, and placing the tissue sieve into the sieve receiving plate;

[0017] S3: adjusting the height of the screen receiving plate through the lifting component, thereby controlling the immersion depth of the tissue slice in the rinse solution;

[0018] S4: The rinsing solution is kept flowing through the circulation component to increase the contact area between the rinsing solution and the tissue sections.

[0019] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects:

[0020] In the above scheme, when it is necessary to drive the rinsing liquid in the rinsing liquid tank, the height of the screen receiving plate and the multiple tissue screens is adjusted by the lifting component, and the multiple tissue screens are lowered into the rinsing liquid tank, so that the multiple rotating shafts and fan blades are located in the gap, and then the first motor is started, and the first motor drives one of the rotating shafts to rotate, and through the action of the pulley and the transmission belt, it can drive the multiple rotating shafts to rotate at the same time, and the multiple rotating shafts drive the circular block and the fan blades to rotate at the same time. During the rotation of the fan blades, the rinsing liquid in the rinsing liquid tank can flow from one end to the other end through the gap, and circulate in sequence, so that the rinsing liquid enhances the rinsing effect on the tissue slices in the tissue screen, and the fan blades can also prevent the rinsing liquid from directly impacting the tissue sample;

[0021] When the tissue screen needs to be rotated, the two rectangular plates are vertically downward under the action of gravity. When the first driving component is started to drive the rinsing liquid in the rinsing liquid box, the rinsing liquid can push the rectangular plate to rotate under the action of the blocking plate, and the rectangular plate can drive the tissue screen to rotate in the placement hole. The tissue screen drives the tissue slice to rotate, so that the tissue section is cleaned more cleanly. When the tissue screen needs to be placed on the platform, the two rectangular plates are rotated in opposite directions respectively, so that the two rectangular plates are parallel, and the tissue screen can be placed on the platform, which is convenient for placing and taking out the tissue slices.

[0022] When the tissue screen needs to be vibrated, the lifting assembly drives the screen receiving plate and the tissue screen to move downward, so that the first circular gear and the second circular gear are meshed with each other, and then the first motor is started, and the output end of the first motor drives the second circular gear to rotate, and the second circular gear drives the first circular gear and the rotating shaft to rotate, and the rotating shaft drives the half-shaped gear to rotate, and the half-shaped gear drives the rack plate to reciprocate left and right, and the rack plate drives the knocking rod to knock the fixed block, so that the fixed block, the connecting frame, the screen receiving plate and the tissue screen vibrate, and the tissue screen can enhance the rinsing effect on the tissue slices during the vibration process;

[0023] When the height of the screen receiving plate needs to be adjusted, start the second motor, and the output end of the second motor drives the worm to rotate, the worm drives the two worm wheels and the screw to rotate, the screw drives the guide block to move, and the guide block drives the screen receiving plate to move along the length direction of the limit rod, so that the height of the screen receiving plate can be adjusted. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, further serve to explain the principles of the invention and to enable those skilled in the relevant art to make and use the invention.

[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0026] Figure 2 It is a schematic diagram of the structure of the vibration assembly and the lifting assembly of the present invention;

[0027] Figure 3 It is a schematic diagram of the placement hole and tissue screen structure of the present invention;

[0028] Figure 4 It is a schematic diagram of the right side cross-sectional structure of the rinse liquid tank of the present invention;

[0029] Figure 5 It is a schematic structural diagram of the first driving assembly of the present invention;

[0030] Figure 6 It is a schematic diagram of the gap structure of the present invention;

[0031] Figure 7 It is a schematic diagram of the structure of the rectangular plate and the blocking plate of the present invention;

[0032] Figure 8 It is a schematic diagram of the first circular gear structure of the present invention;

[0033] Fig. 9 It is a schematic diagram of the structure of the vibration component of the present invention;

[0034] Fig.10 It is a schematic diagram of the structure of the lifting assembly of the present invention;

[0035] Fig.11 For the present invention Fig.10 A is an enlarged structural diagram;

[0036] Fig.12 It is a schematic structural diagram of the receiving assembly and the second driving assembly of the present invention;

[0037] Fig.13 For the present invention Figure 4 Enlarged structural diagram at point B.

[0038] [Reference Signs]

[0039] 101, rinse liquid tank; 102, screen receiving plate; 103, placement hole; 104, tissue screen; 201, rotating shaft; 202, circular block; 203, fan blade; 204, gap; 205, fixed plate; 206, first L-shaped plate; 207, first motor; 208, pulley; 209, transmission belt; 210, semicircular block; 211, connecting shaft; 212, rectangular plate; 213, blocking plate; 214, connecting frame; 215, second L-shaped plate; 216, rotating shaft; 217, semi-shaped gear; 218, rack plate; 219, knocking rod; 220, fixed block; 221, first circular gear; 222, second circular gear; 223, screw ; 224, worm gear; 225, connecting plate; 226, third L-shaped plate; 227, second motor; 228, worm; 229, limit rod; 230, guide block; 231, rectangular block; 232, connecting column; 233, rotating block; 234, first magnet; 235, second magnet; 236, fourth L-shaped plate; 237, rectangular groove; 238, inclined plate; 239, surrounding edge; 240, drain outlet; 241, bottom plate; 242, limit plate; 243, fifth L-shaped plate; 244, third motor; 245, bidirectional threaded rod; 246, movable plate; 247, guide rod; 248, inclined surface; 249, drain hole; 250, rubber plug; 251, pull ring.

[0040] As shown in the figure, in order to clearly implement the structure of the embodiment of the present invention, specific structures and devices are marked in the figure, but this is only for illustrative purposes and is not intended to limit the present invention to the specific structure, device and environment. According to specific needs, ordinary technicians in this field can adjust or modify these devices and environments. DETAILED DESCRIPTION

[0041] The following is a detailed description of a rinsing device and a rinsing method for a tissue section antibody after frozen sectioning provided by the present invention in conjunction with the accompanying drawings and specific embodiments. At the same time, it is explained here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and those skilled in the art may also adopt other alternatives to implement some known technologies; and the accompanying drawings are only for a more specific description of the embodiments, and are not intended to specifically limit the present invention.

[0042] It should be noted that the references to "one embodiment", "embodiment", "exemplary embodiments", "some embodiments" and the like in the specification indicate that the embodiments described may include specific features, structures or characteristics, but not every embodiment may include the specific features, structures or characteristics. In addition, when a specific feature, structure or characteristic is described in conjunction with an embodiment, it should be within the knowledge of a person skilled in the art to implement such feature, structure or characteristic in conjunction with other embodiments (whether or not explicitly described).

[0043] In general, a term can be understood, at least in part, from its use in context. For example, depending, at least in part, on the context, the term "one or more" as used herein can be used to describe any feature, structure, or characteristic in the singular sense, or can be used to describe a combination of features, structures, or characteristics in the plural sense. Additionally, the term "based on" can be understood as not necessarily intended to convey an exclusive set of factors, but can instead, depending, at least in part, on the context, allow for the presence of other factors that are not necessarily explicitly described.

[0044] It will be understood that the meanings of “on,” “over,” and “above” in the present invention should be interpreted in the broadest manner, so that “on” not only means “directly on” something, but also includes the meaning of being “on” something with intervening features or layers therebetween, and “on” or “over” not only means “on” or “above” something, but also includes the meaning of being “on” or “above” something with no intervening features or layers therebetween.

[0045] Additionally, spatially relative terms such as "under," "beneath," "lower," "above," "upper," and the like may be used herein for descriptive convenience to describe the relationship of one element or feature to another element or features, as shown in the accompanying drawings. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the accompanying drawings. The device may be oriented in other ways, and the spatially relative descriptors used herein may be similarly interpreted accordingly.

[0046] like Figures 1 to 13 As shown, an embodiment of the present invention provides a rinsing device and a rinsing method for tissue section antibodies after frozen sectioning, comprising a rinsing liquid tank 101 and a screen receiving plate 102, wherein the screen receiving plate 102 is provided with a plurality of placement holes 103, and a tissue screen 104 is arranged in each of the plurality of placement holes 103, the rinsing liquid tank 101 is provided with a first driving component for driving the rinsing liquid, the screen receiving plate 102 is provided with a vibration component for vibrating the tissue screen 104, the rinsing liquid tank 101 is provided with a lifting component for lifting the screen receiving plate 102, the lifting component is provided with an installation component for installing the screen receiving plate 102, and the rinsing liquid tank 101 is provided with a receiving component for receiving the rinsing liquid.

[0047] When the device is needed, pour a proper amount of rinsing liquid into the rinsing liquid tank 101, then put the tissue screen 104 into the placement hole 103 on the screen receiving plate 102, place the assembled screen receiving plate 102 on the lifting component, fix the screen receiving plate 102 by installing the component, then start the lifting component, the lifting component drives the assembled screen receiving plate 102 to move downward, so that the tissue screen 104 is lowered into the rinsing liquid tank 101, so that the tissue slices in the tissue screen 104 naturally float in the rinsing liquid, start the first driving component and the vibration component, and the rinsing liquid tank 101 is vibrated by the first driving component. The rinsing liquid in the 01 is driven to enhance the rinsing effect of the tissue slices in the tissue screen 104. The driving component can also prevent the rinsing liquid from directly impacting the tissue sample. The vibration component can enhance the rinsing effect of the tissue slices in the tissue screen 104. When the rinsing liquid in the rinsing liquid box 101 needs to be replaced, the rinsing liquid dropped from the tissue screen 104 can be received by the receiving component, which is convenient for replacing the rinsing liquid in the main body and avoiding waiting for a long time. All tissues are sorted on the same screen receiving plate 102, which is convenient for counting and distinguishing the layers of continuously sliced ​​tissues.

[0048] like Figure 4 and Figure 5 As shown, the first driving assembly includes a plurality of rotating shafts 201, and the plurality of rotating shafts 201 are all rotated and penetrate one end of the rinsing liquid tank 101, a circular block 202 is fixedly connected to one side of the rotating shaft 201, and a plurality of fan blades 203 are fixedly connected to the outer surface of the circular block 202, and gaps 204 that cooperate with the plurality of fan blades 203 are provided between the plurality of tissue screens 104, a fixed plate 205 is fixedly connected to one side of the rinsing liquid tank 101, and the plurality of rotating shafts 201 are away from the rinsing liquid tank 101. One side is rotatably connected to the fixed plate 205, one side of the rinse liquid tank 101 is fixedly connected to a first L-shaped plate 206, the inner side of the first L-shaped plate 206 is fixedly connected to a first motor 207, one of the rotating shafts 201 is fixedly connected to the output end of the first motor 207, the output end of the first motor 207 rotates and passes through the fixed plate 205, the outer surfaces of the multiple rotating shafts 201 are fixedly connected to pulleys 208, and the same transmission belt 209 is arranged between the multiple pulleys 208.

[0049] When it is necessary to drive the rinsing liquid in the rinsing liquid box 101, the height of the screen receiving plate 102 and the multiple tissue screens 104 is adjusted by the lifting component, and the multiple tissue screens 104 are lowered into the rinsing liquid box 101, so that the multiple rotating shafts 201 and the fan blades 203 are located in the gap 204, and then the first motor 207 is started, and the first motor 207 drives one of the rotating shafts 201 to rotate, and through the action of the pulley 208 and the transmission belt 209, the multiple rotating shafts 201 can be driven to rotate at the same time, and the multiple rotating shafts 201 simultaneously drive the circular block 202 and the fan blades 203 to rotate. During the rotation of the fan blades 203, the rinsing liquid in the rinsing liquid box 101 can be driven from one end to the other end and circulated in sequence, so that the rinsing liquid enhances the rinsing effect on the tissue sections in the tissue screen 104, and the driving component can also prevent the rinsing liquid from directly impacting the tissue sample.

[0050] like Figure 7 As shown, the bottom ends of the plurality of tissue screens 104 are symmetrically fixedly connected with semicircular blocks 210 at the front and rear ends, a connecting shaft 211 is fixedly connected between two corresponding semicircular blocks 210, the outer surface of the connecting shaft 211 is rotatably connected with a rectangular plate 212, and the bottom end of the tissue screen 104 is fixedly connected with a blocking plate 213, and the blocking plate 213 is located between the two rectangular plates 212.

[0051] When the tissue screen 104 needs to be rotated, the two rectangular plates 212 are vertically downward under the action of gravity. When the first driving component is started to drive the rinsing liquid in the rinsing liquid box 101, under the action of the blocking plate 213, the rinsing liquid can push the rectangular plate 212 to rotate, and the rectangular plate 212 can drive the tissue screen 104 to rotate in the placement hole 103. The tissue screen 104 drives the tissue slices to rotate, so that the tissue section is cleaned more cleanly. When the tissue screen 104 needs to be placed on the platform, the two rectangular plates 212 are rotated in opposite directions respectively, so that the two rectangular plates 212 are parallel, and the tissue screen 104 can be placed on the platform, which is convenient for placing and removing tissue slices.

[0052] like Figure 8 and Fig. 9As shown, the vibration assembly includes a connecting frame 214, which is arranged on the screen receiving plate 102, and the front end of the connecting frame 214 is fixedly connected to the second L-shaped plate 215, and the rear end of the second L-shaped plate 215 is rotatably connected to the connecting frame 214 with a rotating shaft 216, and the outer surface of the rotating shaft 216 is fixedly connected to a half-shaped gear 217, and the front end of the connecting frame 214 is slidably connected to a rack plate 218, and the rack plate 218 is meshed with the rotating shaft 216, and the rack plate 218 is symmetrically fixedly connected to a knocking rod 219 on the left and right, and the front end of the connecting frame 214 is symmetrically fixedly connected to a fixed block 220 on the left and right, and the outer surface of the rotating shaft 216 is fixedly connected to a first circular gear 221, and the output end of the first motor 207 is fixedly connected to a second circular gear 222.

[0053] When the tissue screen 104 needs to be vibrated, the screen receiving plate 102 and the tissue screen 104 are driven downward by the lifting assembly, so that the first circular gear 221 and the second circular gear 222 are meshed with each other, and then the first motor 207 is started, and the output end of the first motor 207 drives the second circular gear 222 to rotate, and the second circular gear 222 drives the first circular gear 221 and the rotating shaft 216 to rotate, and the rotating shaft 216 drives the half-gear 217 to rotate, and the half-gear 217 drives the rack plate 218 to reciprocate left and right, and the rack plate 218 drives the knocking rod 219 to knock the fixed block 220, so that the fixed block 220, the connecting frame 214, the screen receiving plate 102 and the tissue screen 104 vibrate, and the tissue screen 104 can increase the contact area between the rinsing liquid and the tissue slice and the rinsing effect during the vibration process.

[0054] like Fig.10 As shown, the lifting assembly includes two screw rods 223, both of which are rotatably connected to the top of the rinsing liquid tank 101, and the outer surfaces of the two screw rods 223 are fixedly connected to worm gears 224. The rinsing liquid tank 101 is provided with two connecting plates 225, one side of which is fixedly connected to a third L-shaped plate 226, the inner side of the third L-shaped plate 226 is fixedly connected to a second motor 227, and the output end of the second motor 227 is fixedly connected to a worm 228, the worm 228 rotatably passes through one of the connecting plates 225, and the worm 228 cooperates with the two worm gears 224. The top of the rinsing liquid tank 101 is fixedly connected to two limiting rods 229, and the outer surfaces of the screw rods 223 and the limiting rods 229 are provided with guide blocks 230, wherein two of the guide blocks 230 are threadedly connected to the screw rods 223, and the other two screws 223 are slidably connected to the limiting rods 229.

[0055] When the height of the screen receiving plate 102 needs to be adjusted, the second motor 227 is started, and the output end of the second motor 227 drives the worm 228 to rotate, and the worm 228 drives the two worm wheels 224 and the screw 223 to rotate, and the screw 223 drives the guide block 230 to move, and the guide block 230 drives the screen receiving plate 102 to move along the length direction of the limit rod 229, so that the height of the screen receiving plate 102 can be adjusted.

[0056] like Fig.11 As shown, the installation assembly includes four rectangular blocks 231, and the four rectangular blocks 231 are respectively fixedly connected to four guide blocks 230, and the connecting frame 214 is fixedly connected to the two front rectangular blocks 231, and the top ends of the four rectangular blocks 231 are rotatably connected to the connecting columns 232, and the top ends of the connecting columns 232 are fixedly connected to the rotating blocks 233, and one side of the rotating blocks 233 is fixedly connected to the first magnet 234, and the side of the guide block 230 close to the rectangular block 231 is fixedly connected to the second magnet 235, and the four corners of the screen receiving plate 102 are fixedly connected to the fourth L-shaped plate 236, and the fourth L-shaped plate 236 is provided with a rectangular groove 237 that cooperates with the rotating block 233.

[0057] When the screen receiving plate 102 needs to be installed, rotate the rotating block 233 from the horizontal state to the vertical state, and then place the four fourth L-shaped plates 236 on the screen receiving plate 102 on the top of the corresponding four guide blocks 230, so that the rectangular groove 237 on the fourth L-shaped plate 236 passes through the rotating block 233, and then rotate the rotating block 233 again to rotate the rotating block 233 from the vertical state to the horizontal state, so that the first magnet 234 and the second magnet 235 attract each other, thereby restricting the screen receiving plate 102 and facilitating the installation and disassembly of the screen receiving plate 102.

[0058] like Fig.12 As shown, the receiving assembly includes two inclined plates 238 , the top ends of the two inclined plates 238 are fixedly connected with a peripheral edge 239 , a drainage port 240 is formed on the peripheral edge 239 , and a second driving assembly is provided on the rinse liquid tank 101 .

[0059] When the rinsing liquid in the rinsing liquid box 101 needs to be replaced, the screen receiving plate 102 is moved to the upper end of the inclined plate 238 by the lifting assembly, and then the second driving assembly is started to drive the two inclined plates 238 to approach each other so that the two inclined plates 238 are in contact, and the rinsing liquid dropped from the tissue screen 104 is received by the two inclined plates 238 and the surrounding edge 239, and the received rinsing liquid can be discharged through the drain port 240, so as to facilitate the replacement of the rinsing liquid in the main body and avoid waiting for a long time.

[0060] like Fig.12As shown, the second driving assembly includes a bottom plate 241, which is fixedly connected to the bottom end of the rinse liquid tank 101, and the connecting plate 225 is fixedly connected to the top of the bottom plate 241. Four limiting plates 242 are fixedly connected to the top of the bottom plate 241, and one side of one of the limiting plates 242 is fixedly connected to a fifth L-shaped plate 243, and the inner side of the fifth L-shaped plate 243 is fixedly connected to a third motor 244, and the output end of the third motor 244 is fixedly connected to a bidirectional threaded rod 245, and the bidirectional threaded rod 245 rotates and passes through one of the limiting plates 242, and two movable plates 246 are threadedly connected to the bidirectional threaded rod 245, and the two inclined plates 238 are respectively fixedly connected to the movable plates 246, and a guide rod 247 is fixedly connected between the two limiting plates 242, and the two movable plates 246 are both slidably connected to the guide rod 247.

[0061] When it is necessary to drive the two inclined plates 238, the third motor 244 is started, and the output end of the third motor 244 drives the bidirectional threaded rod 245 to rotate, and the bidirectional threaded rod 245 drives the two inclined plates 238 to approach each other along the length direction of the guide rod 247, so that the two inclined plates 238 contact each other, and the rinsing liquid dropped from the tissue screen 104 is received. The received rinsing liquid can be discharged through the drain port 240, which is convenient for replacing the rinsing liquid in the main body and avoids waiting for a long time.

[0062] like Fig.13 As shown, the inner bottom wall of the rinse liquid tank 101 is provided with an inclined surface 248 , and a drainage hole 249 is opened on one side of the rinse liquid tank 101 . A rubber plug 250 is engaged in the drainage hole 249 , and a pull ring 251 is fixedly connected to one side of the rubber plug 250 .

[0063] When the rinsing liquid in the rinsing liquid tank 101 needs to be discharged, the pull ring 251 is pulled, and the pull ring 251 drives the rubber plug 250 to leave the drainage hole 249 , and the rinsing liquid in the rinsing liquid tank 101 is discharged from the drainage hole 249 through the action of the inclined surface 248 .

[0064] like Figure 1 - Fig.13 As shown, S1: adding an appropriate amount of rinsing liquid into the rinsing liquid box 101 to ensure that sufficient rinsing liquid can cover all tissue slices;

[0065] S2: placing the tissue slice into the tissue sieve 104 for carrying, and placing the tissue sieve 104 into the sieve receiving plate 102;

[0066] S3: adjusting the height of the screen receiving plate 102 by means of the lifting assembly, thereby controlling the immersion depth of the tissue slice in the rinse solution;

[0067] S3: The rinsing solution is kept flowing through the circulation component to increase the contact area between the rinsing solution and the tissue sections.

[0068] The specific working process of the technical solution of the present invention is as follows:

[0069] When in use, pour an appropriate amount of rinsing liquid into the rinsing liquid box 101, then place the tissue screen 104 into the placement hole 103 on the screen receiving plate 102, rotate the rotating block 233, and rotate the rotating block 233 from a horizontal state to a vertical state. Then, place the four fourth L-shaped plates 236 on the screen receiving plate 102 on the top of the corresponding four guide blocks 230 respectively, so that the rectangular groove 237 on the fourth L-shaped plate 236 passes through the rotating block 233, and rotate the rotating block 233 again, and rotate the rotating block 233 from a vertical state to a horizontal state, so that the first magnet 234 and the second magnet 235 attract each other, thereby restricting the screen receiving plate 102.

[0070] Start the second motor 227, and the output end of the second motor 227 drives the worm 228 to rotate, and the worm 228 drives the two worm wheels 224 and the screw 223 to rotate, and the screw 223 drives the guide block 230 to move, and the guide block 230 drives the screen receiving plate 102 to move downward along the length direction of the limit rod 229, so that the tissue screen 104 is lowered into the rinse liquid tank 101, so that the tissue sections in the tissue screen 104 naturally float in the rinse liquid, and the screen receiving plate 102 drives the vibration assembly to move downward at the same time during the descending process, and when the tissue screen 104 is lowered into the rinse liquid tank 101, the multiple rotating shafts 201 and the fan blades 203 are located in the gap 204, and the first circular gear 221 and the second circular gear 222 are meshed with each other.

[0071] Start the first motor 207, and the output end of the first motor 207 drives the second circular gear 222 and one of the rotating shafts 201 to rotate simultaneously. Through the action of the pulley 208 and the transmission belt 209, multiple rotating shafts 201 can be driven to rotate simultaneously. Multiple rotating shafts 201 simultaneously drive the circular block 202 and the fan blades 203 to rotate. During the rotation of the fan blades 203, the rinsing liquid in the rinsing liquid tank 101 can be driven from one end to the other end and circulated in sequence, so that the rinsing liquid can enhance the rinsing effect on the tissue sections in the tissue screen 104.

[0072] When the second circular gear 222 rotates, the second circular gear 222 can drive the first circular gear 221 and the rotating shaft 216 to rotate, and the rotating shaft 216 drives the half-shaped gear 217 to rotate. The half-shaped gear 217 drives the rack plate 218 to reciprocate left and right, and the rack plate 218 drives the knocking rod 219 to knock the fixed block 220, so that the fixed block 220, the connecting frame 214, the screen receiving plate 102 and the tissue screen 104 vibrate. The tissue screen 104 can enhance the rinsing effect on the tissue slices during the vibration process. Subsequently, when the rinsing liquid in the rinsing liquid tank 101 is driven, the rinsing liquid can push the rectangular plate 212 to rotate. The rectangular plate 212 can drive the tissue screen 104 to rotate in the placement hole 103, and the tissue screen 104 drives the tissue slices to rotate, so that the tissue sections are cleaned more cleanly. When the rinsing liquid in the rinsing liquid tank 101 needs to be replaced, the second motor 227 is started, and the output end of the second motor 227 drives the worm 228 to rotate, and the worm 228 drives the two worm wheels 224 and the screw 223 to rotate, and the screw 223 drives the guide block 230 to move, and the guide block 230 drives the screen receiving plate 102 to move upward along the length direction of the limit rod 229, so that the tissue screen 104 moves to a position higher than the inclined plate 238.

[0073] Then, the third motor 244 is started, and the output end of the third motor 244 drives the bidirectional threaded rod 245 to rotate, and the bidirectional threaded rod 245 drives the two inclined plates 238 to approach each other along the length direction of the guide rod 247, so that the two inclined plates 238 contact each other, and the rinsing liquid dropped from the tissue screen 104 is received, and the received rinsing liquid can be discharged through the drain port 240, and the pull ring 251 is pulled, and the pull ring 251 drives the rubber plug 250 to leave the drain hole 249, and the rinsing liquid in the rinsing liquid tank 101 is discharged from the drain hole 249 through the action of the inclined surface 248.

[0074] The present invention covers any substitution, modification, equivalent method and scheme made on the essence and scope of the present invention. In order to make the public have a thorough understanding of the present invention, specific details are described in detail in the following preferred embodiments of the present invention, but those skilled in the art can fully understand the present invention without the description of these details. In addition, in order to avoid unnecessary confusion about the essence of the present invention, well-known methods, processes, procedures, components and circuits are not described in detail.

[0075] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A rinsing device for tissue section antibodies after frozen sectioning, comprising a rinsing liquid tank and a screen receiving plate, characterized in that: The screen receiving plate is provided with a plurality of placement holes, each of the plurality of placement holes being provided with a tissue screen, the rinse liquid tank is provided with a first driving component for driving the rinse liquid, the screen receiving plate is provided with a vibration component for vibrating the tissue screen, the rinse liquid tank is provided with a lifting component for lifting the screen receiving plate, the lifting component is provided with an installation component for installing the screen receiving plate, and the rinse liquid tank is provided with a receiving component for receiving the rinse liquid.

2. The rinsing device for antibodies in tissue sections after frozen sections according to claim 1, characterized in that: The first driving component includes multiple rotating shafts, multiple rotating shafts all rotate and pass through one end of the rinsing liquid tank, a circular block is fixedly connected to one side of the rotating shaft, and multiple fan blades are fixedly connected to the outer surface of the circular block, and gaps that cooperate with the multiple fan blades are arranged between the multiple tissue screens, a fixed plate is fixedly connected to one side of the rinsing liquid tank, and multiple rotating shafts are rotatably connected to the fixed plate on one side away from the rinsing liquid tank, a first L-shaped plate is fixedly connected to one side of the rinsing liquid tank, and a first motor is fixedly connected to the inner side of the first L-shaped plate, one of the rotating shafts is fixedly connected to the output end of the first motor, and the output end of the first motor rotates and passes through the fixed plate, and the outer surfaces of the multiple rotating shafts are fixedly connected to pulleys, and the same transmission belt is arranged between the multiple pulleys.

3. The rinsing device for antibodies in tissue sections after frozen sections according to claim 2, characterized in that: The bottom ends of the multiple tissue screens are symmetrically fixedly connected with semicircular blocks at the front and back, a connecting shaft is fixedly connected between two corresponding semicircular blocks, the outer surface of the connecting shaft is rotatably connected with a rectangular plate, and the bottom end of the tissue screen is fixedly connected with a blocking plate, and the blocking plate is located between the two rectangular plates.

4. The rinsing device for antibodies in tissue sections after frozen sections according to claim 3, characterized in that: The vibration assembly includes a connecting frame, which is arranged on a screen receiving plate, a second L-shaped plate is fixedly connected to the front end of the connecting frame, a rotating shaft is rotatably connected between the rear end of the second L-shaped plate and the connecting frame, a half-shaped gear is fixedly connected to the outer surface of the rotating shaft, a rack plate is slidably connected to the front end of the connecting frame, the rack plate and the rotating shaft are meshed with each other, a knocking rod is symmetrically fixedly connected to the rack plate on the left and right, a fixed block is symmetrically fixedly connected to the front end of the connecting frame on the left and right, a first circular gear is fixedly connected to the outer surface of the rotating shaft, and a second circular gear is fixedly connected to the output end of the first motor.

5. The rinsing device for antibodies in tissue sections after frozen sections according to claim 4, characterized in that: The lifting assembly includes two screw rods, both of which are rotatably connected to the top of the rinsing liquid tank, and the outer surfaces of the two screw rods are fixedly connected to worm gears. Two connecting plates are provided on the rinsing liquid tank, and a third L-shaped plate is fixedly connected to one side of one of the connecting plates, and a second motor is fixedly connected to the inner side of the third L-shaped plate, and a worm is fixedly connected to the output end of the second motor. The worm rotates and passes through one of the connecting plates, and the worm cooperates with the two worm gears. Two limit rods are fixedly connected to the top of the rinsing liquid tank, and guide blocks are provided on the outer surfaces of the screw rods and the limit rods, two of which are threadedly connected to the screw rods, and the other two screws are slidably connected to the limit rods.

6. The rinsing device for antibodies in tissue sections after frozen sections according to claim 5, characterized in that: The mounting assembly includes four rectangular blocks, which are respectively fixedly connected to four guide blocks, the connecting frame is fixedly connected to the two front rectangular blocks, the top ends of the four rectangular blocks are rotatably connected to connecting columns, the top ends of the connecting columns are fixedly connected to a rotating block, one side of the rotating block is fixedly connected to a first magnet, the side of the guide block close to the rectangular block is fixedly connected to a second magnet, and the four corners of the screen receiving plate are fixedly connected to a fourth L-shaped plate, and the fourth L-shaped plate is provided with a rectangular groove that cooperates with the rotating block.

7. The rinsing device for antibodies in tissue sections after frozen sections according to claim 6, characterized in that: The receiving assembly includes two inclined plates, the top ends of the two inclined plates are fixedly connected with a peripheral edge, a drainage port is provided on the peripheral edge, and a second driving assembly is arranged on the rinse liquid tank.

8. The rinsing device for antibodies in tissue sections after frozen sections according to claim 7, characterized in that: The second driving assembly includes a base plate, which is fixedly connected to the bottom end of the rinse liquid tank, the connecting plate is fixedly connected to the top of the base plate, and four limit plates are fixedly connected to the top of the base plate, one side of one of the limit plates is fixedly connected to a fifth L-shaped plate, the inner side of the fifth L-shaped plate is fixedly connected to a third motor, and the output end of the third motor is fixedly connected to a bidirectional threaded rod, the bidirectional threaded rod rotates and passes through one of the limit plates, and two movable plates are threadedly connected to the bidirectional threaded rod, and the two inclined plates are respectively fixedly connected to the movable plates, wherein a guide rod is fixedly connected between the two limit plates, and the two movable plates are both slidably connected to the guide rod.

9. The rinsing device for antibodies in tissue sections after frozen sections according to claim 8, characterized in that: The inner bottom wall of the rinse liquid tank is provided with an inclined surface, and a drainage hole is opened on one side of the rinse liquid tank. A rubber plug is clamped in the drainage hole, and a pull ring is fixedly connected to one side of the rubber plug.

10. A rinsing method for a tissue section antibody rinsing device after frozen sectioning according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: Add an appropriate amount of rinse solution into the rinse solution box to ensure that there is enough rinse solution to cover all tissue sections; S2: placing the tissue slice into the tissue sieve for carrying, and placing the tissue sieve into the sieve receiving plate; S3: adjusting the height of the screen receiving plate through the lifting component, thereby controlling the immersion depth of the tissue slice in the rinse solution; S4: The rinsing solution is kept flowing through the circulation component to increase the contact area between the rinsing solution and the tissue sections.

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