Awnless brome callus induction medium and application thereof

By optimizing the ratio and conditions of callus induction medium for the callus induction medium of Mangle-free seeds, the problem of long and low efficiency induction of callus in the prior art was solved, and efficient callus induction was achieved , supports the rapid reproduction and genetic engineering applications of Wumeng Choumai.

CN119488064APending Publication Date: 2025-02-21INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Application Number
CN202411909179.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-24
Publication Date
2025-02-21

AI Technical Summary

Technical Problem

The prior art has problems such as poor material selection, long induction time and long differentiation time in the induction and genetic transformation of callus in the seeds of Mangle Mai, which limits the in-depth understanding of the molecular biological characteristics of Mangle Mai.

Method used

Provide a callus induction medium without marsh, including MS basic medium, sucrose, agar, 6-BA and 2,4-D. By optimizing the media ratio and conditions, the seeds of marsh, Callus.

Benefits of technology

By optimizing the culture medium and conditions, the induction efficiency of callus seeds of Mangle-free Cyclope is significantly improved, and is suitable for rapid reproduction, germplasm resource preservation and genetic engineering applications of Mangle-free Cyclope.

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Abstract

The invention relates to a tissue culture technology, in particular to an awnless brome callus induction culture medium and application thereof, and the awnless brome callus induction culture medium is characterized by comprising MS, sucrose, agar, 6-BA and 2, 4-D. The induction is performed by using awnless brome seeds. The awnless brome seed callus is cultivated, a new experimental material is provided for molecular biology and genetic engineering research of awnless brome, and a solid foundation is laid for molecular biology research of awnless brome. Finally, according to the culture medium, the hormone proportion and variety difference are optimized, more calluses can be induced to be generated, the overall induction efficiency is improved, the quality of the calluses can be improved, the growth stability of the seed calluses can be kept through accurately controlled environmental conditions, the difference between batches is reduced, and the yield of the seed calluses is increased. Therefore, the method is more suitable for subsequent awnless brome regeneration or genetic engineering application.
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Citation Information

Patent Citations

  • Regeneration method of Bromus inermis using hypocotyl as explant

    CN117730781B

  • Tissue culture regeneration method of mature awnless brome seeds

    CN104137774A

  • Method for regenerating awnless brome by taking hypocotyl as explant

    CN117730781A