Extraction method of edible torreya grandis protein

Abstract

The invention provides an extraction method of edible torreya grandis protein, which comprises the following steps: step 1, shelling raw torreya grandis seeds, removing impurities, and cleaning to obtain raw torreya grandis pulp; step 2, crushing the raw torreya grandis pulp by using an ultrafine crusher to obtain torreya grandis powder; step 3, mixing the Chinese torreya powder with petroleum ether, stirring, and performing suction filtration by using a suction filtration device to obtain degreased Chinese torreya powder; step 4, mixing the degreased Chinese torreya powder with deionized water, stirring, and centrifuging by using a centrifugal machine to obtain a supernatant and a precipitate; and step 5, mixing the precipitate with deionized water, stirring, centrifuging by using a centrifugal machine, taking supernate and the precipitate, mixing the obtained supernate, and repeating the step 3-5 times. According to the method, protein in the torreya grandis seeds can be fully extracted while grease is squeezed from the torreya grandis seeds, usable torreya grandis protein is generated to serve as food or food additives, high-quality food and food additives with nutritive value are provided for the majority of people, especially patients, extracted waste can be used for extracting torreya grandis oil, the process is simple, and the method is suitable for industrial production. Operation is convenient, and the protein extraction rate is high.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and particularly relates to a method for extracting edible Torreya grandis protein. Background Art

[0002] Torreya grandis is a unique dried fruit tree species in China. It is native to Zhejiang Province and is currently mainly produced in southeastern China. It is an ecological and economic tree species that can maintain water and soil and conserve water resources. Torreya grandis seeds are extremely nutritious and high-quality dried fruits. The kernels have a high oil content. The seeds can be used to extract edible oil and can be used to produce lubricants and waxes.

[0003] However, what is often overlooked is that Torreya grandis seeds are also rich in protein, vitamins, minerals and other nutrients, especially protein, which shows high edible and medicinal value in anti-inflammatory, anti-hypertensive, anti-tumor and sterilization. The amino acids contained in its protein are similar to the amino acid recommended by the World Health Organization and the Food and Agriculture Organization of the United Nations, and it is a protein with high nutritional value. Torreya grandis protein is also of fine quality, good taste and unique flavor, making it very suitable as a nutritional additive for patients.

[0004] As mentioned above, oil extraction, production of lubricants and waxes are the common industrial uses of Torreya grandis seeds both at home and abroad. In the above production process, the defatted Torreya grandis raw materials will be directly discarded, resulting in a large waste of nutrients.

[0005] Therefore, in order to extract edible protein from Torreya grandis seeds and avoid the waste of nutrients, it is necessary to provide a method for extracting a large amount of edible Torreya grandis protein from Torreya grandis seeds. Summary of the invention

[0006] Based on the above-mentioned deficiencies of the prior art, the present invention provides a method for extracting edible Torreya grandis protein. The method can fully extract the protein in Torreya grandis seeds while squeezing oil from Torreya grandis seeds, thereby producing Torreya grandis protein that can be used as food or food additive. The waste after extraction can also be used for the extraction of Torreya grandis oil, thereby solving the technical problems mentioned in the prior art.

[0007] A method for extracting edible Torreya grandis protein, characterized in that the method comprises the following steps:

[0008] Step 1, shelling, removing impurities and washing the raw Torreya grandis seeds to obtain raw Torreya grandis pulp;

[0009] Step 2: Use an ultrafine grinder to grind the raw Torreya grandis pulp to obtain Torreya grandis powder;

[0010] Step 3, mixing and stirring the Torreya grandis powder and petroleum ether, and filtering using a suction filtration device to obtain defatted Torreya grandis powder;

[0011] Step 4, mixing and stirring the defatted Torreya grandis powder and deionized water, and centrifuging using a centrifuge to obtain a supernatant and a precipitate;

[0012] Step 5, mixing the precipitate with deionized water, stirring, centrifuging using a centrifuge, taking the supernatant and the precipitate, mixing the obtained supernatants, and repeating this step 3-5 times;

[0013] Step 6, adding HCl solution to the mixed supernatant obtained in step 5, adjusting the pH value of the supernatant to 3.5, standing and centrifuging, and collecting Torreya grandis protein precipitate;

[0014] Step 7, adding the collected Torreya grandis protein precipitate to deionized water for redissolution, adding NaOH solution to adjust to neutrality, stirring at room temperature, and obtaining a Torreya grandis protein solution after the Torreya grandis protein precipitate is completely dissolved;

[0015] Step 8, placing the Torreya grandis protein solution into a dialysis bag of 8000-14000Da, and dialyzing the dialysis bag in deionized water for 48 hours to obtain a Torreya grandis protein dialysate;

[0016] Step 9: freeze-dry the dialyzate to obtain Torreya grandis protein.

[0017] In this technical solution, preferably, in step 3, the mass ratio of Torreya grandis powder to petroleum ether is 1:5 to 1:10.

[0018] In this technical solution, preferably, in steps 3, 4 and 5, stirring is performed using a magnetic stirrer at room temperature for 2 hours or longer.

[0019] In this technical solution, preferably, in step 4, the mass ratio of defatted Torreya grandis powder to deionized water is 1:10.

[0020] In this technical solution, preferably, in step 5, the mass ratio of the precipitate to the deionized water is 1:5 to 1:20.

[0021] In this technical solution, preferably, in steps 4 and 5, the centrifugal speed is 10000 rpm, the centrifugal temperature is 4° C., and the centrifugal time is greater than or equal to 15 minutes.

[0022] In this technical solution, preferably, in step 6, the concentration of the HCl solution is 2 mol / L.

[0023] In this technical solution, preferably, in step 6, the standing time is greater than or equal to 30 minutes, the centrifugal speed is 8000 rpm, the centrifugal temperature is 4° C., and the centrifugal time is greater than or equal to 10 minutes.

[0024] In this technical solution, preferably, in step 7, the mass ratio of Torreya grandis protein precipitate and ionized water is 1:5.

[0025] In this technical solution, preferably, in step 7, the concentration of the NaOH solution is 1 mol / L.

[0026] The method for extracting edible Torreya grandis protein provided by the present invention can fully extract protein from Torreya grandis seeds while squeezing oil from Torreya grandis seeds, thereby producing usable Torreya grandis protein as food or food additive, providing high-quality food and food additive with nutritional value for the general public, especially patients. The waste after extraction can also be used for extracting Torreya grandis oil. The process is simple, easy to operate, and has a high protein extraction rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a flow chart of Example 1 of the method for extracting edible Torreya grandis protein of the present invention;

[0028] Figure 2 It is the extracted edible Torreya grandis protein powder. DETAILED DESCRIPTION

[0029] In order to make the technical content of the present invention more easily understood, the technical solution of the present invention is described in detail below in conjunction with embodiments.

[0030] Example 1

[0031] In Example 1, a method for extracting edible Torreya grandis protein is provided. Figure 1The method first peels the raw Torreya grandis seeds, removes impurities, and cleans them to obtain raw Torreya grandis pulp raw material. Then, the raw Torreya grandis pulp is crushed for 2 hours using a food-grade ultrafine grinder to obtain Torreya grandis powder. Then, defatting is performed, Torreya grandis powder is mixed with petroleum ether at a mass ratio of 1:5, and magnetic stirring is performed for 4 hours. After stirring, Torreya grandis powder and petroleum ether are fully mixed, and a suction filtration device is used for suction filtration to obtain defatted Torreya grandis powder. The waste liquid obtained after suction filtration can be used for the extraction of Torreya grandis oil. Then, the defatted Torreya grandis powder is mixed with deionized water at a mass ratio of 1:10, magnetic stirring is performed for 3 hours, and centrifugation is performed using a centrifuge to obtain a supernatant and a precipitate. Then the precipitate was mixed with deionized water at a mass ratio of 1:10, stirred, and centrifuged using a centrifuge, with a centrifugal speed of 10000rpm, a centrifugal temperature of 4°C, and a centrifugal time of 15 minutes. The supernatant and the precipitate were taken, and the obtained supernatant was mixed, and then the precipitate was mixed with deionized water for several times, stirred, and centrifuged, with a centrifugal speed of 10000rpm, a centrifugal temperature of 4°C, and a centrifugal time of 45 minutes. After repeating 5 times, all the supernatants were mixed. Then, a 2 mol / L HCl solution was added to the obtained mixed supernatant, and the pH value of the supernatant was adjusted to 3.5, and the mixture was allowed to stand for 30 minutes, and then centrifuged at a centrifugal speed of 8000rpm, a centrifugal temperature of 4°C, and a centrifugal time of 10 minutes to collect the Torreya grandis protein precipitate. Then the collected Torreya grandis protein precipitate was added to deionized water for re-dissolution, the mass ratio of Torreya grandis protein precipitate to deionized water was 1:5, 1 mol / L NaOH solution was added to adjust to neutrality, stirred at room temperature, and after the Torreya grandis protein precipitate was completely dissolved, a Torreya grandis protein solution was obtained. The Torreya grandis protein solution was placed in an 8000Da dialysis bag, and the dialysis bag was dialyzed in deionized water for 48 hours to obtain a Torreya grandis protein dialysate. The dialysate was freeze-dried to finally obtain Torreya grandis protein.

[0032] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution, which should be included in the scope of the claims of the present invention.

Claims

1. A method for extracting edible Torreya grandis protein, characterized in that: The method comprises the following steps: Step 1, shelling, removing impurities and washing the raw Torreya grandis seeds to obtain raw Torreya grandis pulp; Step 2: Use an ultrafine grinder to grind the raw Torreya grandis pulp to obtain Torreya grandis powder; Step 3, mixing and stirring the Torreya grandis powder and petroleum ether, and filtering using a suction filtration device to obtain defatted Torreya grandis powder; Step 4, mixing and stirring the defatted Torreya grandis powder and deionized water, and centrifuging using a centrifuge to obtain a supernatant and a precipitate; Step 5, mixing the precipitate with deionized water, stirring, centrifuging using a centrifuge, taking the supernatant and the precipitate, mixing the obtained supernatant, and repeating this step 3-5 times; Step 6, adding HCl solution to the mixed supernatant obtained in step 5, adjusting the pH value of the supernatant to 3.5, standing and centrifuging, and collecting Torreya grandis protein precipitate; Step 7, adding the collected Torreya grandis protein precipitate to deionized water for redissolution, adding NaOH solution to adjust to neutrality, stirring at room temperature, and obtaining a Torreya grandis protein solution after the Torreya grandis protein precipitate is completely dissolved; Step 8, placing the Torreya grandis protein solution into a dialysis bag of 8000-14000Da, and dialyzing the dialysis bag in deionized water for 48 hours to obtain a Torreya grandis protein dialysate; Step 9: freeze-dry the dialyzate to obtain Torreya grandis protein.

2. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In step 3, the mass ratio of Torreya grandis powder to petroleum ether is 1:5 to 1:

10.

3. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In steps 3, 4 and 5, stirring is performed using a magnetic stirrer at room temperature for 2 hours or longer.

4. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In step 4, the mass ratio of defatted Torreya grandis powder to deionized water is 1:

10.

5. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In step 5, the mass ratio of the precipitate to the deionized water is 1:5 to 1:

20.

6. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In steps 4 and 5, the centrifugal speed is 10000 rpm, the centrifugal temperature is 4° C., and the centrifugal time is greater than or equal to 15 minutes.

7. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In step 6, the concentration of the HCl solution is 2 mol / L.

8. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In step 6, the standing time is greater than or equal to 30 minutes, the centrifugal speed is 8000 rpm, the centrifugal temperature is 4° C., and the centrifugal time is greater than or equal to 10 minutes.

9. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In step 7, the mass ratio of Torreya grandis protein precipitate to ionized water is 1:

5.

10. The method for extracting edible Torreya grandis protein according to claim 1, characterized in that: In step 7, the concentration of the NaOH solution is 1 mol / L.

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