Control preparation for northern corn leaf blight and application of control preparation

Abstract

The invention relates to a combined control agent for northern corn leaf blight, which comprises active bacteria, chelated zinc brassicae and a propiconazole-azoxystrobin suspending agent, and the active bacteria are bacillus cereus or fusarium avenaceum. When the bacillus cereus or the fusarium avenaceum is compounded with the zinc brassicae chelate, the dosage of propiconazole-azoxystrobin can be reduced, so that the relative control effect on northern corn leaf blight is greatly improved, the yield of corn is also increased, and the highest yield increase rate can reach 13.9%.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides and relates to a prevention and treatment preparation for corn leaf blight and application thereof. Background Art

[0002] Corn is an important food crop. Corn leaf spot (Corynes poracassiicola), also known as corn spot, is a disease caused by the pathogen Corynes poracassiicola. The pathogen invades corn leaves through stomata or wounds, and the hyphae expand inside the leaves and produce large spots on the leaves. Its metabolites also destroy the cell structure of the plant, causing the leaves to wilt and fall, thereby affecting photosynthesis and reducing corn yields. Studies have found that the spores of the pathogen are spread through air, water flow, seeds and other channels, and can spread rapidly under suitable environmental conditions, especially in environments with high humidity. The spread speed is fast, and if effective prevention and control measures are not taken, a large area of ​​leaves will be damaged. In addition to being harmful to corn, the pathogen Corynes poracassiicola can cause pathogenic damage to other perennial crops. Currently, commonly used fungicides include chlorfenapyr, thiophanate-methyl, chlorfenapyr, etc., but there is also a problem of drug resistance. Summary of the invention

[0003] In view of this, the object of the present invention is to provide a control preparation for corn leaf blight.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] 1. Application of Bacillus cereus or Fusarium solani in the preparation of a prevention and control preparation for corn leaf blight.

[0006] 2. Provide a combined control agent for corn leaf blight, which comprises active bacteria, chelated zinc and propiconazole-pyraclostrobin suspension.

[0007] Furthermore, in the combined control agent, the active bacteria is Bacillus cereus or Fusarium solani.

[0008] Furthermore, in the combined control agent, the chelated zinc is Brassica chelated zinc.

[0009] Furthermore, in the combined control agent, the propiconazole·azoxystrobin suspension concentrate is a 18.7% propiconazole·azoxystrobin suspension concentrate.

[0010] Furthermore, in the combined control agent, the dosage concentration of each component in the combined control agent is: active bacteria 400-800mL / hm 2 , chelated zinc 450mL / hm 2 -550mL / hm 2, Propiconazole·Azoxystrobin suspension 800mL / hm 2 -1000mL / hm 2 .

[0011] The concentration of active bacteria is 10 10 -10 11 cfu / mL.

[0012] Furthermore, in the combined control agent, the dosage concentration of each component in the combined control agent is: active bacteria 600-800mL / hm 2 , chelated zinc 450mL / hm 2 , Propiconazole·Azoxystrobin suspension 800mL / hm 2 .

[0013] 3. It also provides the use of combined control agents in inhibiting plant leaf spot disease.

[0014] Specifically, the combined control agent includes active bacteria, chelated zinc and propiconazole-pyraclostrobin suspension.

[0015] The active bacteria are Bacillus cereus or Fusarium avenae.

[0016] Furthermore, the plant is corn.

[0017] Further in the application, the dosage concentration of each component in the combined control agent is: active bacteria 600-800mL / hm 2 , chelated zinc 450mL / hm 2 , Propiconazole·Azoxystrobin suspension 800mL / hm 2 .

[0018] The beneficial effect of the present invention is that the present invention can significantly improve the relative control effect of corn leaf blight by using Bacillus cereus or Fusarium solani with brassica chelated zinc and propiconazole·pyraclostrobin in combination. When the dosage of propiconazole·pyraclostrobin is reduced, the disease index is significantly lower than that of using propiconazole·pyraclostrobin alone. When the disease index of the blank control is 18.57, the disease index of the agent of the present invention is only 3.49-4.42 after use, which has a significant control or prevention effect on corn leaf blight. At the same time, the dosage of propiconazole·pyraclostrobin is relatively reduced by 20%, reducing pollution to the environment. The relative control effect is 76.2%-81.2%, the yield is increased by 8.7-13.9%, it has good economic and ecological benefits, and has excellent application prospects for the control of corn leaf blight. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to make the purpose, technical solution and beneficial effects of the present invention clearer, the present invention provides the following drawings for illustration:

[0020] Figure 1 This is a photo of the blank control test site.

[0021] Figure 2 18.7% propiconazole·azoxystrobin suspension 1000mL / hm 2 Processing photos of the test site.

[0022] Figure 3 Fusarium solani 600ml / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Photographs of the treated test plots. DETAILED DESCRIPTION

[0023] The preferred embodiment of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. The experimental methods for which specific conditions are not specified in the embodiments are usually carried out under conventional conditions or under conditions recommended by the manufacturer.

[0024] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0025] 18.7% propiconazole·azoxystrobin suspension: Syngenta Crop Protection GmbH, Switzerland;

[0026] Brassica chelated zinc: North American Agricultural University Group;

[0027] Example 1

[0028] 1. Experimental materials: The test was conducted on the corn variety Xianyu 335 susceptible to northern leaf spot disease. The test was divided into 3 blocks (3 replicates), each with 12 treatments, and randomized block arrangement was used.

[0029] 2. Activate Bacillus cereus and Fusarium avenae and culture them to the logarithmic phase, dilute to 10 10 -10 11 cfu / mL was stored for future use. The pollution-free preparations (Bacillus cereus / Fusarium solani, Brassica chelated zinc) were sprayed at the jointing stage of corn, and the application period of 18.7% propiconazole·pyraclostrobin suspension was the small trumpet stage, and the total volume of the application at each period was 800L / hm 2 The corn leaves were sprayed with the pathogen of U. maydis at the trumpet stage. The conidia concentration in the inoculation suspension was 1×10 5 / ml~1×10 6 / mL, the inoculation amount is controlled at 5-10mL / plant. The occurrence of corn leaf spot was investigated during the milky stage. The investigation method is to randomly select 20-25 plants in each plot, investigate the disease level of all its leaves according to the standard, and calculate the disease index and relative control effect. After the corn matures, select the middle section of each plot, collect 1 ear for every 5 ears, and harvest a total of 30 ears. After threshing and drying, the yield is measured.

[0030] Relative control effect (%) = (disease index of blank control area - disease index of treated area) / disease index of blank control area × 100%

[0031] 3. A total of 12 treatment methods were designed, namely T1: Bacillus licheniformis 600mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T2: Bacillus thuringiensis 600mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T3: Bacillus licheniformis 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T4: Bacillus thuringiensis 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T5: Bacillus licheniformis 400mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T6: Bacillus thuringiensis 400mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T7: Bacillus licheniformis 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T8: Bacillus thuringiensis 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T9: Brassica chelated zinc 450mL / hm 2+18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 , T10: 18.7% propiconazole·pyraclostrobin suspension 800mL / hm 2 (reduced by 20%), T11: 18.7% propiconazole·pyraclostrobin suspension 1000mL / hm 2 (conventional dosage), T12: blank control test.

[0032] The specific classification standards for corn leaf blight are as follows:

[0033] Level 0: no disease;

[0034] Level 1: The lesion area accounts for less than 5% of the leaf area;

[0035] Level 3: The lesion area accounts for 6%-10% of the leaf area;

[0036] Level 5: The lesion area accounts for 11%-25% of the leaf area;

[0037] Level 7: The lesion area accounts for 26%-50% of the leaf area;

[0038] Level 9: The lesion area accounts for more than 51% of the leaf area.

[0039] Example 2

[0040] The test results of the combined application of the pollution-free agent and 18.7% propiconazole·azoxystrobin suspension are shown in Table 1:

[0041]

[0042]

[0043] Figure 1 This is a photo of the blank control test site. It can be seen from the details that the area of ​​lesions accounts for more than 50% of the leaf area; Figure 2 18.7% propiconazole·azoxystrobin suspension 1000mL / hm 2 Processing photos of the test site; Figure 3 Fusarium solani 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 The results in Table 1 show that when the disease index of the blank control was 18.57, the avenaceae Fusarium 400mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2Treatment, Bacillus cereus 400mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Fusarium solani 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Fusarium solani 800mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 800mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, 18.7% propiconazole·azoxystrobin suspension 1000mL / hm 2 The disease indexes of the treatment of large spot disease were all below 5, namely 4.10 and 4.4 respectively. 2 , 3.49, 3.99, 3.86, 3.94 and 4.57, with control effects of 77.9%, 76.2%, 81.2%, 78.5%, 79.2%, 78.8% and 75.4% respectively, while reducing the dosage of propiconazole·azoxystrobin suspension by 18.7%, 800mL / hm 2 The disease index of the treatment was 5.11, and the control effect was 72.5%. At the same dosage of 18.7% propiconazole·pyraclostrobin suspension, when only combined with avenae Fusarium solani or Bacillus cereus, the disease index and control effect were equivalent, only 5.07 and 5.26, and the relative control effects were 72.7 and 71.7 respectively.

[0044] According to variance analysis, Fusarium solani 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Fusarium solani 800mL / hm2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 800mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 The disease index of the treatment was significantly lower than that of Fusarium solani 600 mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 600mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Fusarium solani 400mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 400mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, 18.7% propiconazole·azoxystrobin suspension 800mL / hm 2 Treatment, 18.7% propiconazole·azoxystrobin suspension 1000mL / hm 2 The disease index of the treated animals was significantly lower than that of the blank control.

[0045] Corn yield relative to blank control 8315.0 kmL / hm 2 Fusarium solani 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 600mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Fusarium solani 800mL / hm 2 + Brassica chelated zinc 450mL / hm 2+18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 The yield increase rates of the treatments were all above 10%, namely 13.9%, 10.2% and 12.8%, and the yields were 9473kg / hm 2 、9163kg / hm 2 、9130kg / hm 2 Relatively speaking, the treatment of Fusarium solani combined with Brassica chelated zinc and propiconazole·pyraclostrobin suspension was more significant.

[0046] Fusarium solani 600mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 600mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Fusarium solani 400mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 400mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Bacillus cereus 800mL / hm 2 + Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, Brassica chelated zinc 450mL / hm 2 +18.7% Propiconazole·Azoxystrobin Suspension 800mL / hm 2 Treatment, 18.7% propiconazole·azoxystrobin suspension 800mL / hm 2 Treatment, 18.7% propiconazole·azoxystrobin suspension 1000mL / hm 2 The treatment output is 8939kg / hm 2 、8847kg / hm 2 、907 2 kg / hm 2 、9038kg / hm 2 、9130kg / hm 2 、8606kg / hm 2 、8914kg / hm 2 , 90 2 2kg / hm 2The yield increase rates were 7.5%, 6.4%, 9.1%, 8.7%, 9.8%, 3.5%, 7.2% and 8.5%, respectively, all of which were improved, indicating that the treatment with Fusarium solani or Bacillus cereus combined with Brassica chelated zinc and propiconazole·pyraclostrobin suspension achieved better prevention and control effects on corn leaf blight, and relatively reduced the dosage of propiconazole·pyraclostrobin suspension by 18.7%.

[0047] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. Application of Bacillus cereus or Fusarium solani in the preparation of a prevention and control preparation for corn leaf blight.

2. A combined control agent for corn leaf blight, characterized in that: The combined control agent comprises active bacteria, chelated zinc and propiconazole-pyraclostrobin.

3. The combined control agent according to claim 1, characterized in that: The active bacteria are Bacillus cereus or Fusarium avenae.

4. The combined control agent according to claim 1, characterized in that: The chelated zinc is brassica chelated zinc.

5. The combined control agent according to claim 1, characterized in that: The propiconazole·azoxystrobin is a 18.7% propiconazole·azoxystrobin suspension.

6. The combined control agent according to any one of claims 1 to 4, characterized in that: The dosage concentration of each component in the combined control agent is: active bacteria 400-800mL / hm 2 , chelated zinc 450mL / hm 2 -550 mL / hm 2 , Propiconazole·Azoxystrobin suspension 800mL / hm 2 -1000 mL / hm 2 .

7. The combined control agent according to claim 5, characterized in that: The dosage concentration of each component in the combined control agent is: active bacteria 600-800mL / hm 2 , chelated zinc 450mL / hm 2 , Propiconazole·Azoxystrobin suspension 800mL / hm 2 .

8. Use of the combined control agent according to any one of claims 1 to 6 in inhibiting plant leaf spot disease.

9. The use according to claim 7, characterized in that: The plant is corn.

10. The use according to claim 7, characterized in that: The dosage concentration of each component in the combined control agent is: active bacteria 400-800mL / hm 2 , chelated zinc 450mL / hm 2 , Propiconazole·Azoxystrobin suspension 800mL / hm 2 .

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