Application of aglaia odorata stem and leaf essential oil in preparation of cosmetics

Abstract

The invention provides application of aglaia odorata stem and leaf essential oil in preparation of cosmetics, and belongs to the technical field of cosmetic development. The aglaia odorata stem and leaf essential oil is extracted by adopting a steam distillation method, and the aglaia odorata stem and leaf essential oil is found to have the effects of inhibiting fungi, inhibiting tyrosinase activity and promoting collagen secretion. The invention lays a material foundation for further exploring the function and biological activity of the aglaia odorata stem and leaf essential oil, and provides a scientific basis for reasonably developing and utilizing the plant resource. The aglaia odorata stem and leaf essential oil disclosed by the invention can achieve the effects of resisting bacteria, whitening and tendering skin as an effective component of cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetic development, and in particular relates to application of orchid stem and leaf essential oil in preparing cosmetics. Background Art

[0002] Aglaia odorata Lour., also known as Milan, tree orchid, and fish orchid, is a plant of the genus Aglaia in the Meliaceae family. It is an evergreen shrub or small tree with many branched stems and a fragrance similar to orchid.

[0003] Since the flowers of the iris are rich in volatile oils, essences can be extracted, so they become high-grade fragrance raw materials for preparing perfumes, soaps and cosmetics, and are also very good clove agents. The roots, stems, leaves, flowers and fruits of the iris can all be used as medicine, which has the functions of promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, and promoting qi and relieving depression. The branches and leaves of the iris are pungent and sweet, and are neutral in nature. They enter the lungs, stomach and liver meridians. The pharmacological effects include sobering up, clearing the lungs, refreshing the head and eyes, and relieving thirst. Therefore, they are often used in traditional Chinese medicine or health foods, and can also purify indoor air. The wood of the iris is yellow and dense, so it is often used for carving, furniture and other materials.

[0004] There are not many reports on the biological activities of the chemical components in the Mizilan plant. The main activities discovered so far include anti-tumor, anti-viral and anti-inflammatory activities. Summary of the invention

[0005] In view of this, the object of the present invention is to provide an application of the essential oil of the stems and leaves of the Artemisia selengensis in the preparation of cosmetics. As a cosmetic, the essential oil of the stems and leaves of the Artemisia selengensis can play an antibacterial, whitening and skin-rejuvenating role.

[0006] Preferably, the Myrica ovata stem and leaf essential oil is made from fresh Myrica ovata stem and leaves as raw materials through steam distillation.

[0007] More preferably, the steam distillation method comprises the following steps: crushing and sieving fresh stems and leaves of Orchidaceae, adding water at a ratio of 1g:3-4mL of solid to liquid, maintaining the distillation extraction for 3.5h-4.5h in a slightly boiling state, collecting the distillate, and removing the water to obtain the Orchidaceae stem and leaf essential oil.

[0008] More preferably, the crushing and sieving are performed until the particle size of the stems and leaves of the orchid is ≤10 mesh.

[0009] More preferably, the distillate is treated with anhydrous sodium sulfate to remove moisture.

[0010] Preferably, the cosmetics include lotions, creams, masks, sprays, essences, essential oils and soaps.

[0011] The invention also provides a cosmetic with antibacterial, whitening and skin-rejuvenating functions, which contains 0.01%-100% of the essential oil of the stems and leaves of the orchid.

[0012] Preferably, the antibacterial activity includes inhibiting Epidermophyton floccosum, Microsporum gypseum and Trichophyton rubrum.

[0013] Preferably, the whitening comprises inhibiting tyrosinase activity.

[0014] Preferably, the skin rejuvenation includes promoting collagen secretion.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The invention lays a material foundation for further exploring the functions and biological activities of the essential oil of the stems and leaves of the orchid, and provides a scientific basis for the rational development and utilization of this plant resource. As a common plant, the stems and leaves of the orchid are widely available and the cost is relatively low.

[0017] The essential oil from the stems and leaves of Artemisia selengensis has an inhibitory effect on common pathogenic fungi such as Epidermophyton floccosum, Microsporum gypseum and Trichophyton rubrum. This makes cosmetics containing this essential oil effective in preventing and improving skin infections caused by these fungi, such as tinea corporis, tinea cruris, tinea manuum and pedis, etc., providing antibacterial protection for the skin and keeping the skin healthy.

[0018] The essential oil of the stem and leaf of Mizilan can inhibit the activity of tyrosinase and reduce the production of melanin, thus helping to reduce spots, brighten the skin tone, and make the skin fairer and more translucent, meeting consumers' demand for whitening cosmetics. The essential oil of the stem and leaf of Mizilan can also promote the secretion of collagen, helping to improve the elasticity and texture of the skin. DETAILED DESCRIPTION

[0019] The present invention provides an application of Aglaia odorata Lour. stem and leaf essential oil in preparing cosmetics. Preferably, the Aglaia odorata Lour. stem and leaf essential oil includes Aglaia odorata stem and branch essential oil, Aglaia odorata leaf essential oil or a mixture of the two. The present invention finds that the Aglaia odorata stem and leaf essential oil has the effects of inhibiting fungi, inhibiting tyrosinase activity and promoting collagen secretion, and can achieve antibacterial, whitening and skin rejuvenation effects as an effective ingredient of cosmetics.

[0020] In the present invention, it is preferred that the stem and leaf essential oil of Mizilan is made from fresh Mizilan stems and leaves as raw materials by steam distillation; further preferably, the steam distillation method comprises the following steps: fresh Mizilan stems and leaves are crushed and sieved, water is added at a ratio of 1g:3-4mL of solid-liquid ratio, more preferably the solid-liquid ratio is 1g:4mL, and the distillation is maintained in a slightly boiling state for 3.5h-4.5h, more preferably for 4h of extraction, and the distillate is collected, and the Mizilan stem and leaf essential oil is obtained after removing water. In the present invention, it is preferred to crush and sieve to a particle size of ≤10 mesh of Mizilan stems and leaves; preferably, the distillate is treated with anhydrous sodium sulfate to remove water.

[0021] In the present invention, preferred cosmetics include lotions, creams, masks, sprays, essences, essential oils and soaps. The present invention has no special restrictions on other raw materials and preparation methods of various types of cosmetics, and the commonly used raw materials and preparation processes in the field can be used.

[0022] The present invention also provides a cosmetic with antibacterial, whitening and skin-rejuvenating functions, which contains 0.01%-100% (mass concentration) of the essential oil of the stems and leaves of the genus Myrtillus. In the present invention, the antibacterial function preferably includes inhibiting Epidermophyton floccosum, Microsporum gypseum and Trichophyton rubrum; the whitening function preferably includes inhibiting tyrosinase activity; and the skin-rejuvenating function preferably includes promoting collagen secretion.

[0023] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0024] Example 1

[0025] Preparation of essential oil from stems and leaves of Mizilan

[0026] Weigh fresh stems and leaves of Orchidaceae, crush them through a 10-mesh sieve, add water at a solid-liquid ratio of 1g:4mL, place them in a 10L round-bottom flask, heat them with an electric heating mantle, maintain a slightly boiling state for distillation and extraction for 4 hours, stop heating, collect the distillate after cooling, and dry it with anhydrous sodium sulfate to obtain Orchidaceae stem and leaf essential oil.

[0027] Example 2

[0028] The difference from Example 1 is that during the steam distillation extraction process, the solid-liquid ratio is 1 g:3 mL and the extraction time is 3.5 h.

[0029] Example 3

[0030] The difference from Example 1 is that during the steam distillation extraction process, the solid-liquid ratio is 1 g:3.5 mL and the extraction time is 4.5 h.

[0031] Example 4

[0032] A cymbidium orchid stem and leaf essential oil emulsion, which is prepared from the following components in percentage by weight:

[0033] Tween 60 2%, glycerin 3%, avocado oil 10%, disodium EDTA 0.01%, shea butter 2%, butylene glycol 6%, the essential oil of the stem and leaf of Artemisia selengensis in Example 1 0.5%, and the rest is ultrapure water.

[0034] The preparation method of the essential oil emulsion is a conventional process in the industry.

[0035] Example 5

[0036] A cymbidium orchid stem and leaf essential oil facial mask, comprising the following components in percentage by mass:

[0037] Glycerin 3%, polyethylene glycol 1%, propylene glycol 1%, avocado oil 2%, Carbomer 9411%, sodium hyaluronate 0.1%, disodium EDTA 0.01%, panthenol 1%, the essential oil of the stems and leaves of Mizigzag orchid in Example 1 0.5%, and the rest is ultrapure water.

[0038] The preparation method of the essential oil facial mask is a conventional process in the industry.

[0039] Example 6

[0040] A Mizilan stem and leaf essential oil spray, which is made from the following components in percentage by mass:

[0041] 5% of isobutane, 25% of vodka, 5% of ethylhexyl methoxycinnamate, 2% of glycerol, 0.2% of sodium hyaluronate, 5% of the essential oil of the stem and leaf of Orchidaceae in Example 1, and the rest of ultrapure water.

[0042] The preparation method of the essential oil spray is a conventional process in the industry.

[0043] Example 7

[0044] A kind of orchid stem and leaf essential oil soap, made from the following components in parts by weight:

[0045] Coconut oil 100g, glycerin 50g, avocado oil 100g, shea butter 50g, sodium hydroxide 50g, ultrapure water 150mL, and the essential oil of the stem and leaf of Mizilan of Example 1 1g.

[0046] The preparation method of the essential oil soap is a conventional process in the industry.

[0047] Example 8

[0048] A kind of orchid stem and leaf essential oil cream, is made from the following components in percentage by weight:

[0049] Stearic acid 7.5%, nonionic emulsifier 4.5%, cyclopentasiloxane (DC-345) 2.3%, lanolin 5%, white oil 5%, glycerin 4.5%, the essential oil of Mizilan stem and leaf of Example 1 5%, 1.3-butylene glycol essence 3%, and the rest is deionized water.

[0050] The preparation method of the essential oil cream is a conventional process in the industry.

[0051] Prepare the essential oil of Mizilan stem and branch and the essential oil of Mizilan leaf respectively, and the preparation method is as follows:

[0052] Weigh fresh stems and leaves of Mizilan, crush them through a 10-mesh sieve, add water at a solid-liquid ratio of 1g:4mL, place in a 10L round-bottom flask, heat with an electric heating mantle, maintain a slightly boiling state for distillation and extraction for 4h, stop heating, collect the distillate after cooling, and dry with anhydrous sodium sulfate to obtain Mizilan stem essential oil (MZL-S) and Mizilan leaf essential oil (MZL-L). The obtained essential oils were packed in brown essential oil bottles and stored in a 4°C refrigerator for subsequent antibacterial tests, tyrosinase activity inhibition tests, and collagen secretion promotion tests.

[0053] Example 9

[0054] Antibacterial test of essential oil from stems and leaves of Orchidaceae

[0055] 1. Test samples and strains

[0056] Prepare the test samples of essential oils from stems and leaves of Orchidaceae according to Table 1.

[0057] Table 1 Antibacterial test sample information

[0058] Sample name Solvents Concentration (μg / mL) MZL-L DMSO 128 MZL-S DMSO 128

[0059] The test strains were four fungi: Epidermophyton floccosum CBS566.94, Trichophyton rubrum ATCC 4438, Microsporum gypseum CBS118893, purchased from the Chinese Academy of Medical Sciences Medical Fungi Collection Center; Candida albicans ATCC 10231 purchased from Microbiologics, Inc., USA.

[0060] 2. Reagents

[0061] Terbinafine hydrochloride, amphotericin B, and DMSO were purchased from Sigma.

[0062] 3. Test methods

[0063] Take a 96-well culture plate, dilute the sample to be tested, add fungal liquid to each well, and the final concentration is 1×10 5 CFU / mL (Candida albicans) and 5×10 5 CFU / mL (filamentous fungi - Epidermophyton floccosum, Trichophyton rubrum, Microsporum gypseum), Candida albicans was cultured at 37℃ for 24h, filamentous fungi were cultured at 25℃ for 5d, and the absorbance at 625nm was detected by ELISA.

[0064] The experiment also set up a culture medium blank control, a fungal control, and amphotericin B and terbinafine hydrochloride positive drug controls.

[0065] 4. Test results

[0066] As shown in Table 2, among the two sample essential oils in this test, the essential oil of Mizilan leaf (MZL-L) has a strong inhibitory effect on Epidermophyton floccosum and Microsporum gypseum; the essential oil of Mizilan stem and branch (MZL-S) has a strong inhibitory effect on Epidermophyton floccosum and Microsporum gypseum, and has a relatively strong inhibitory effect on Trichophyton rubrum.

[0067] Table 2 The inhibitory effect of the essential oil of Mizilan stem and leaf on fungi

[0068]

[0069] Example 10

[0070] Inhibition test of tyrosinase activity of essential oil from stems and leaves of Orchidaceae

[0071] 1. Test samples

[0072] Prepare the test samples of essential oils from stems and leaves of Orchidaceae according to Table 3.

[0073] Table 3 Tyrosinase activity inhibition test sample information

[0074] Sample name Solvents Concentration (μg / mL) MZL-L DMSO 100 MZL-S DMSO 100

[0075] 2. Reagents

[0076] Phenethyl resorcinol (377) was purchased from Aladdin Reagent (Shanghai) Co., Ltd.; L-Dopa was purchased from Sigma.

[0077] 3. Test methods

[0078] The sample to be tested was mixed with L-Dopa (final concentration 1.25 mM), and tyrosinase (final concentration 25 U / mL) was added to start the reaction. Three replicate wells were set, and a blank control without drug and a positive control of phenylethylresorcinol (377) were set at the same time. The OD value was measured by a microplate reader at room temperature for 5 minutes, and the detection wavelength was 490 nm. The tyrosinase activity inhibition rate was calculated.

[0079] Tyrosinase activity inhibition rate (%) = [1-sample OD 490 / OD of experimental control well 490 ]×100

[0080] 4. Test results

[0081] As shown in Table 4, the essential oil of the leaves of Mizilan (MZL-L) has a strong inhibitory effect on tyrosinase, and the inhibition rate is more than 40%.

[0082] Table 4 Inhibitory effect of the essential oil of Mizilan stem and leaf on tyrosinase activity

[0083] sample Concentration (μg / mL) Tyrosinase inhibition rate (%) 377 0.1 70.125±1.339 MZL-L 100 40.614±1.013 MZL-S 100 12.491±1.89

[0084] Embodiment 11

[0085] Experiment on promoting collagen secretion by essential oil from stems and leaves of Orchidaceae

[0086] 1. Test samples

[0087] Prepare the test samples of essential oils from stems and leaves of Orchidaceae according to Table 5.

[0088] Table 5 Sample information of the test for promoting collagen secretion

[0089] Sample name Solvents Concentration (μg / mL) MZL-L DMSO 1 MZL-S DMSO 1

[0090] 2. Cells and Reagents

[0091] Human dermal fibroblasts-adult (HDFa) were purchased from ATCC.

[0092] Transforming growth factor beta (TGF-β) was purchased from Peprotech; collagen ELISA kit was purchased from TaKaRa; MTS reagent was purchased from Promega.

[0093] 3. Test methods

[0094] HDFa cells were mixed with the samples to be tested on a 96-well cell culture plate, and a blank control without drugs and a TGF-β positive control were set up; the cells were cultured at 37°C and 5% CO2 for 3 days, and the cell culture supernatant was collected and stored at -80°C; MTS was added, and the OD value at 490nm was detected by MTS colorimetry; the secretion of collagen was detected according to the method provided in the collagen ELISA kit, and the OD value was measured by a microplate reader with a detection wavelength of 450nm. The increase rate of collagen secretion was calculated.

[0095] Collagen secretion increase rate (%) = (OD 450 / Cell viability / blank well OD 450 -1 )×100%

[0096] 4. Test results

[0097] As shown in Table 6, both M. zilch stem and branch essential oil (MZL-S) and M. zilch leaf essential oil (MZL-L) had a weak promoting effect on HDFa collagen secretion.

[0098] Table 6 Effect of promoting collagen secretion of Mizilan stem and leaf essential oil

[0099] sample Concentration (μg / mL) Cell survival rate (%) Increase rate of collagen secretion (%) TGF-β 0.01 123.091 107.301±3.07 MZL-L 1 107.406 12.452±2.565 MZL-S 1 109.510 16.813±2.097

[0100] From the above, it can be seen that the essential oil of the stems and leaves of Mizilan has the effects of inhibiting fungi, inhibiting tyrosinase activity and promoting collagen secretion. The development of cosmetics can achieve antibacterial and whitening effects.

[0101] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Application of the essential oil of stems and leaves of Mizilan in the preparation of cosmetics.

2. The use according to claim 1, characterized in that: The iris orchid stem and leaf essential oil is prepared from fresh iris orchid stem and leaf as raw materials through steam distillation.

3. The use according to claim 2, characterized in that: The steam distillation method comprises the following steps: crushing and sieving fresh stems and leaves of Orchidaceae, adding water at a ratio of 1g:3-4mL of solid to liquid, maintaining a slightly boiling state for distillation and extraction for 3.5h-4.5h, collecting the distillate, and removing water to obtain the Orchidaceae stem and leaf essential oil.

4. The use according to claim 3, characterized in that: The crushing and screening are performed until the particle size of the stems and leaves of the mizilan is ≤10 meshes.

5. The use according to claim 3, characterized in that: The distillate is treated with anhydrous sodium sulfate to remove moisture.

6. The use according to claim 1, characterized in that: The cosmetics include lotions, creams, facial masks, sprays, essences, essential oils and soaps.

7. A cosmetic with antibacterial, whitening and skin-rejuvenating functions, characterized in that: The cosmetic contains 0.01%-100% of the essential oil of the stem and leaf of Orchidaceae according to any one of claims 1 to 3.

8. The cosmetic according to claim 7, characterized in that: The antibacterial activity includes inhibition of Epidermophyton floccosum, Microsporum gypseum and Trichophyton rubrum.

9. The cosmetic according to claim 7, characterized in that: The whitening includes inhibiting tyrosinase activity.

10. The cosmetic according to claim 7, characterized in that: The skin rejuvenation includes promoting collagen secretion.

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