Skin care composition containing perigord truffle and plant exosome and application of skin care composition

Abstract

The invention relates to a skin care composition containing perigord truffle and plant exosome and application of the skin care composition. The skin care composition containing perigord truffle and plant exosome comprises perigord truffle extract, chlorella exosome, malachite extract, fermentation product filtrate and soluble collagen. The fermentation product filtrate comprises any one or a combination of at least two of a Thermus thermophilus fermentation product, a lactobacillus / soybean milk fermentation product filtrate or a lactobacillus / mung bean seed extract / sodium glutamate fermentation product filtrate. The perigord truffle extract, the chlorella exosome, the malachite extract, the fermentation product filtrate and the soluble collagen are creatively combined to form the composition with the whitening, anti-allergy and anti-oxidation effects, and the composition has the effects of inhibiting melanin synthesis, relieving skin inflammation response, whitening skin, resisting allergy and resisting oxidation. And an unexpected synergistic effect is achieved in the aspects of oxidation resistance and aging resistance. The composition is mild in performance and free of irritation and toxic and side effects, and can be applied to cosmetic products more safely.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a skin care composition comprising black truffle and plant exosomes and an application thereof. Background Art

[0002] With the development and progress of society, people are paying more and more attention to the external manifestation of personal image and health status. However, due to the continuous contact with the outside world, including external pollution, ultraviolet radiation and other factors, the skin will not only have wrinkles, sagging and pigmentation, but also reduce the metabolism of the epidermis, weaken the ability of the epidermis to repair damage and the barrier protection ability. Therefore, in order to maintain the health of the skin during daily skin care, skin care products with multiple functions are needed.

[0003] CN117338626A discloses an anti-aging, whitening and moisturizing composition and a preparation method thereof, which belongs to the field of cosmetic technology. After the wall of Dendrobium officinale, oats and sea buckthorn is broken, enzymolysis is performed, filtration is performed, the solid is retained, zinc salt and calcium salt are added to the filtrate to obtain a protein peptide-amino acid chelated Zn-Ca complex; the solid is mixed with Tremella fuciformis and ginseng, extracted with boiling water, filtered, the first solid is retained, alcohol precipitation is performed to obtain a composite polysaccharide, licorice is subjected to supercritical fluid extraction to obtain licorice essential oil, the solid is mixed with the first solid, inoculated with fermentation bacteria for fermentation, filtered, the filtrate is extracted and purified, and dried to obtain an active extract, which is mixed with a protein peptide-amino acid chelated Zn-Ca complex, a composite polysaccharide, licorice essential oil, sodium hyaluronate, niacinamide, and a peptide to obtain an anti-aging, whitening and moisturizing composition, which has good antioxidant, whitening, anti-inflammatory and anti-allergic effects, has a repairing effect on damaged skin barriers, and has broad application prospects.

[0004] CN116747179A discloses a whitening, anti-allergic and moisturizing composition, which is prepared by mixing and compounding traditional Chinese medicines, and the traditional Chinese medicines are selected from one or more of peach kernel, safflower, angelica, chuanxiong, white peony root, prepared rehmannia root, zedoaria, motherwort, pollen, salvia miltiorrhiza, notoginseng, millettia spatholobi, etc. The composition is compounded with conventional whitening and antioxidant raw materials such as glabridin, 377, arbutin and astaxanthin, etc., which can synergize and reduce the irritation of the above ingredients, remove spots and whiten the skin, make the skin ruddy and shiny, and have antioxidant and anti-ultraviolet effects.

[0005] Although the above-mentioned cosmetics have multiple functions such as whitening, moisturizing, anti-allergic, and anti-oxidation, due to the barrier function of human skin itself, it is difficult for general active ingredients to truly penetrate deep into the skin to exert their effects. Therefore, it is necessary to develop a safe, environmentally friendly, and efficient product that has multiple functions such as anti-aging, brightening, and anti-allergic to meet the needs of consumers. Summary of the invention

[0006] In view of the deficiencies in the prior art, an object of the present invention is to provide a skin care composition comprising black truffle and plant exosomes and application thereof.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a skin care composition comprising black truffle and plant exosomes, wherein the skin care composition comprising black truffle and plant exosomes comprises black truffle extract, chlorella exosomes, malachite extract, fermentation product filtrate and soluble collagen;

[0009] The fermentation product filtrate includes any one of Thermus thermophilus fermentation product, Lactobacillus / soymilk fermentation product filtrate or Lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate or a combination of at least two thereof.

[0010] The black truffle extract in the composition of the present invention is rich in amino acids and polysaccharides, which can inhibit the generation of free radicals, whiten and moisturize the skin, and has a significant anti-allergic effect, can calm the skin and reduce inflammatory reactions; the chlorella exosomes can promote the synthesis of collagen and elastin, thereby improving the brightness and uniformity of the skin, and it also has excellent antioxidant and soothing effects; the natural copper and other trace minerals in the malachite extract can provide antioxidant protection, help the skin resist the damage of free radicals, thereby reducing the occurrence of allergic reactions, and can also accelerate the microcirculation inside the skin, remove old dead skin cells, improve skin color, and restore the skin to a transparent and healthy base color; the soluble collagen can provide sufficient nutrients for the skin, regulate skin metabolic activities, and promote the excretion of metabolic substances to avoid the occurrence of large amounts of local skin melanin deposition; the fermentation product filtrate can inhibit the generation of melanin, thereby reducing skin spots and dullness, making the skin look brighter and more uniform, and the fermentation product filtrate also contains anti-inflammatory and anti-allergic ingredients, which can reduce the inflammatory reaction of the skin. The present invention selects the above five materials for use in combination, which has a synergistic effect, and the effective ingredients can penetrate into the skin better, so that the composition can have excellent whitening, anti-allergic and antioxidant effects.

[0011] Preferably, the skin care composition comprising black truffle and plant exosomes comprises, by weight, 1-3 parts of black truffle extract, 1-2 parts of chlorella exosomes, 1-2 parts of malachite extract, 5-10 parts of fermentation product filtrate and 3-6 parts of soluble collagen.

[0012] The amount of the black truffle extract added to the skin care composition comprising black truffle and plant exosomes of the present invention can be 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts, 2 parts, 2.1 parts, 2.3 parts, 2.5 parts, 2.7 parts or 2.9 parts, etc.;

[0013] The addition amount of the Chlorella exosomes can be 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts or 1.9 parts, etc.;

[0014] The amount of the malachite extract added can be 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts or 1.9 parts, etc.;

[0015] The fermentation product filtrate may be added in an amount of 5.2, 5.5, 5.8, 6, 6.2, 6.5, 6.8, 7, 7.2, 7.5, 7.8, 8, 8.2, 8.5, 8.8, 9, 9.2, 9.5 or 9.8, etc.;

[0016] The added amount of the soluble collagen can be 3.1 parts, 3.3 parts, 3.5 parts, 3.7 parts, 3.9 parts, 4 parts, 4.1 parts, 4.3 parts, 4.5 parts, 4.7 parts, 5.0 parts, 5.2 parts, 5.5 parts or 5.8 parts, etc.

[0017] Preferably, the fermentation product filtrate includes a Thermus thermophilus fermentation product, a Lactobacillus / soymilk fermentation product filtrate, and a Lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate.

[0018] The Thermus thermophilus fermentation product, Lactobacillus / soymilk fermentation product filtrate or Lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate in the present invention not only have excellent moisturizing and antioxidant effects, but also contain a variety of anti-inflammatory and anti-allergic ingredients, which can reduce the inflammatory response of the skin and relieve skin sensitivity. The combination of the three can make the above-mentioned effects of the composition more significant.

[0019] Preferably, the mass ratio of the Thermus thermophilus fermentation product, the Lactobacillus / soymilk fermentation product filtrate and the Lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate is (1-3):(1-2):(1-2).

[0020] Wherein "1-3" can be 1.1, 1.3, 1.5, 1.7, 1.9, 2, 2.1, 2.3, 2.5, 2.7 or 2.9, etc.;

[0021] The first "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0022] The second "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0023] Preferably, the skin care composition comprising black truffle and plant exosomes also comprises superoxide dismutase.

[0024] Preferably, the skin care composition comprising black truffle and plant exosomes further comprises 0.1-2 parts of superoxide dismutase by weight, for example, 0.2 parts, 0.5 parts, 0.7 parts, 0.9 parts, 1 parts, 1.2 parts, 1.4 parts, 1.5 parts, 1.7 parts or 1.9 parts, etc.

[0025] Preferably, the skin care composition comprising black truffle and plant exosomes also includes sodium DNA.

[0026] By adding superoxide dismutase and sodium DNA to the composition of the present invention, the two work synergistically, so that the composition can better promote the metabolism and regeneration of skin cells, inhibit the generation of free radicals, and reduce the frequency of binding between allergens and antibodies, reduce the occurrence of skin allergic reactions, thereby making the whitening, anti-allergic and anti-oxidation effects of the composition more significant.

[0027] Preferably, the skin care composition containing black truffle and plant exosomes also includes 0.1-1 parts of sodium DNA by weight, for example, it can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or 0.9 parts, etc.

[0028] Preferably, the skin care composition comprising black truffle and plant exosomes also includes a polypeptide.

[0029] Preferably, the skin care composition comprising black truffle and plant exosomes further comprises 2-4 parts of polypeptides by weight, for example, 2.1 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.7 parts or 3.9 parts, etc.

[0030] Preferably, the polypeptide comprises any one of palmitoyl tripeptide-8, tetrapeptide-1 or nonapeptide-1, or a combination of at least two thereof.

[0031] Preferably, the polypeptides include palmitoyl tripeptide-8, tetrapeptide-1 and nonapeptide-1.

[0032] As a preferred technical solution of the present invention, the palmitoyl tripeptide-8, tetrapeptide-1 and nonapeptide-1 cooperate synergistically to better promote the synthesis of collagen in the skin, neutralize free radicals, and reduce skin inflammatory reactions. After being added to the composition of the present invention, the whitening, antioxidant and anti-allergic effects of the composition can be made more significant.

[0033] Preferably, the mass ratio of palmitoyl tripeptide-8, tetrapeptide-1 and nonapeptide-1 can be (2-4):(1-3):(1-4).

[0034] Wherein "2-4" can be 2.1, 2.3, 2.5, 2.7, 2.9, 3, 3.1, 3.3, 3.5, 3.7 or 3.9, etc.;

[0035] "1-3" can be 1.1, 1.3, 1.5, 1.7, 1.9, 2, 2.1, 2.3, 2.5, 2.7 or 2.9, etc.;

[0036] “1-4” can be 1.2, 1.4, 1.6, 1.8, 2, 2.2, 2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6 or 3.8, etc.

[0037] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0038] In a second aspect, the present invention provides a method for preparing a skin care composition comprising black truffle and plant exosomes as described in the first aspect, the preparation method comprising the following steps: mixing and stirring black truffle extract, chlorella exosomes, malachite extract, fermentation product filtrate and soluble collagen to obtain the skin care composition comprising black truffle and plant exosomes.

[0039] Preferably, the preparation method comprises:

[0040] (1) mixing black truffle extract, Chlorella exosomes, fermentation product filtrate and water to obtain solution A;

[0041] (2) mixing the soluble collagen, superoxide dismutase, and polypeptide with the solution A obtained in step (1) to obtain a solution B;

[0042] (3) Finally, the malachite extract, sodium DNA and solution B obtained in step (2) are mixed to obtain the skin care composition containing black truffle and plant exosomes.

[0043] Preferably, the mixing temperature in step (1) is 40-50°C (for example, 40°C, 42°C, 45°C, 48°C or 50°C, etc.), the mixing is carried out in a stirring manner, the stirring time is 0.5-1h (for example, 0.6h, 0.7h, 0.8h, 0.9h or 1h, etc.), and the stirring speed is 1000-2000rpm (for example, 1100rpm, 1300rpm, 1500rpm, 1700rpm or 1900rpm, etc.).

[0044] Preferably, the mixing temperature in step (2) is 40-50°C (for example, 40°C, 42°C, 45°C, 48°C or 50°C, etc.), the mixing is carried out in a stirring manner, the stirring time is 0.5-1h (for example, 0.6h, 0.7h, 0.8h, 0.9h or 1h, etc.), and the stirring speed is 1000-2000rpm (for example, 1100rpm, 1300rpm, 1500rpm, 1700rpm or 1900rpm, etc.).

[0045] Preferably, the mixing temperature in step (3) is 35-45°C (for example, it can be 36°C, 38°C, 40°C, 42°C or 44°C, etc.), the mixing is carried out in a stirring manner, the stirring time is 20-40min (for example, it can be 22min, 25min, 28min, 30min, 32min, 35min or 38min, etc.), and the stirring speed is 1000-2000rpm (for example, it can be 1100rpm, 1300rpm, 1500rpm, 1700rpm or 1900rpm, etc.).

[0046] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0047] In a third aspect, the present invention provides a use of a skin care composition comprising black truffle and plant exosomes as described in the first aspect in the preparation of cosmetics.

[0048] Preferably, the cosmetics include any one of essence, essence milk, face cream, face cleanser or lotion.

[0049] Preferably, the mass percentage of the skin care composition comprising black truffle and plant exosomes in the cosmetic is 1-20%, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18% or 19%, etc.

[0050] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0051] Compared with the prior art, the present invention has the following beneficial effects:

[0052] The present invention creatively combines black truffle extract, chlorella exosomes, malachite extract, fermentation product filtrate and soluble collagen to form a composition with whitening, anti-allergy and antioxidant effects. The five combinations of black truffle extract, chlorella exosomes, malachite extract, fermentation product filtrate and soluble collagen in the composition are scientifically matched, and have unexpected synergistic effects in inhibiting melanin synthesis, reducing skin inflammatory reactions, and anti-oxidation and anti-aging. In addition, the composition is mild and non-irritating, has no toxic side effects, and can be more safely applied to cosmetic products. DETAILED DESCRIPTION

[0053] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0054] The weight percentages involved in the following embodiments and comparative examples are all calculated based on the actual effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0055] The following materials and comparative examples are as follows:

[0056]

[0057]

[0058] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0059] Example 1

[0060] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the skin care composition comprising black truffle and plant exosomes comprises, by weight, 2 parts of black truffle extract, 1.5 parts of chlorella exosomes, 1.5 parts of malachite extract, 8 parts of fermentation product filtrate, 5 parts of soluble collagen, 1 part of superoxide dismutase, 0.5 parts of sodium DNA, 3 parts of polypeptide, and water is added to 50 parts;

[0061] The fermentation product filtrate is a Thermus thermophilus fermentation product, a Lactobacillus / soymilk fermentation product filtrate, and a Lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate in a mass ratio of 2:1.5:1.5;

[0062] The polypeptides are palmitoyl tripeptide-8, tetrapeptide-1 and nonapeptide-1 in a mass ratio of 3:2:3.

[0063] The preparation method of the skin care composition comprising black truffle and plant exosomes is:

[0064] (1) mixing black truffle extract, Chlorella exosomes, fermentation product filtrate and water, and stirring at 45° C. and 1500 rpm for 0.8 h to obtain solution A;

[0065] (2) mixing the soluble collagen, superoxide dismutase, and polypeptide with the solution A obtained in step (1), and stirring at 45° C. and 1500 rpm for 0.8 h to obtain a solution B;

[0066] (3) Finally, the malachite extract, sodium DNA and solution B obtained in step (2) are mixed, and stirred at 40° C. and 1500 rpm for 30 min to obtain the skin care composition containing black truffle and plant exosomes.

[0067] Example 2

[0068] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the skin care composition comprising black truffle and plant exosomes comprises, by weight, 3 parts of black truffle extract, 1 part of chlorella exosomes, 2 parts of malachite extract, 5 parts of fermentation product filtrate, 6 parts of soluble collagen, 0.5 parts of superoxide dismutase, 1 part of sodium DNA, 2 parts of polypeptide, and water is added to 50 parts;

[0069] The fermentation product filtrate is a Thermus thermophilus fermentation product, a Lactobacillus / soymilk fermentation product filtrate, and a Lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate in a mass ratio of 3:1:2;

[0070] The polypeptides are palmitoyl tripeptide-8, tetrapeptide-1 and nonapeptide-1 in a mass ratio of 4:1:4.

[0071] The preparation method of the skin care composition comprising black truffle and plant exosomes is:

[0072] (1) mixing black truffle extract, Chlorella exosomes, fermentation product filtrate and water, and stirring at 50° C. and 1000 rpm for 0.5 h to obtain solution A;

[0073] (2) mixing the soluble collagen, superoxide dismutase, and polypeptide with the solution A obtained in step (1), and stirring at 40° C. and 2000 rpm for 1 h to obtain a solution B;

[0074] (3) Finally, the malachite extract, sodium DNA and solution B obtained in step (2) are mixed, and stirred at 45° C. and 1000 rpm for 20 min to obtain the skin care composition containing black truffle and plant exosomes.

[0075] Example 3

[0076] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the skin care composition comprising black truffle and plant exosomes comprises, by weight, 1 part of black truffle extract, 2 parts of chlorella exosomes, 1 part of malachite extract, 9 parts of fermentation product filtrate, 3 parts of soluble collagen, 2 parts of superoxide dismutase, 0.3 parts of sodium DNA, 4 parts of polypeptide, and water is added to 50 parts;

[0077] The fermentation product filtrate is a Thermus thermophilus fermentation product, a Lactobacillus / soymilk fermentation product filtrate, and a Lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate in a mass ratio of 1:2:1;

[0078] The polypeptides are palmitoyl tripeptide-8, tetrapeptide-1 and nonapeptide-1 in a mass ratio of 2:3:1.

[0079] The preparation method of the skin care composition comprising black truffle and plant exosomes is:

[0080] (1) mixing black truffle extract, Chlorella exosomes, fermentation product filtrate and water, and stirring at 40° C. and 2000 rpm for 1 h to obtain solution A;

[0081] (2) mixing the soluble collagen, superoxide dismutase, and polypeptide with the solution A obtained in step (1), and stirring at 50° C. and 1000 rpm for 0.5 h to obtain a solution B;

[0082] (3) Finally, the malachite extract, sodium DNA and solution B obtained in step (2) are mixed, and stirred at 35° C. and 2000 rpm for 40 min to obtain the skin care composition containing black truffle and plant exosomes.

[0083] Example 4

[0084] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the components of the skin care composition comprising black truffle and plant exosomes do not include superoxide dismutase, and the reduced amount thereof is supplemented by sodium DNA, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0085] Example 5

[0086] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the components of the skin care composition comprising black truffle and plant exosomes do not include sodium DNA, and the reduced amount thereof is supplemented by superoxide dismutase, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0087] Example 6

[0088] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the components of the skin care composition comprising black truffle and plant exosomes do not include polypeptides, and the reduced amount thereof is supplemented by water, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0089] Example 7

[0090] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the polypeptides in the skin care composition comprising black truffle and plant exosomes are palmitoyl tripeptide-8 and tetrapeptide-1 in a mass ratio of 3:2, and the added amount of the polypeptides is kept unchanged, and the other components and added amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0091] Example 8

[0092] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the polypeptides in the skin care composition comprising black truffle and plant exosomes are tetrapeptide-1 and nonapeptide-1 in a mass ratio of 2:3, and the added amount of the polypeptides is kept unchanged, and the other components and added amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0093] Example 9

[0094] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the polypeptides in the skin care composition comprising black truffle and plant exosomes are palmitoyl tripeptide-8 and nonapeptide-1 in a mass ratio of 1:1, and the added amount of the polypeptides is kept unchanged, and the other components and added amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0095] Example 10

[0096] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the fermentation product filtrate in the skin care composition comprising black truffle and plant exosomes is a fermentation product of Thermus thermophilus and a filtrate of a Lactobacillus / soy milk fermentation product in a mass ratio of 2:1.5, and the addition amount of the fermentation product filtrate is kept unchanged, and the other components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0097] Embodiment 11

[0098] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the fermentation product filtrate in the skin care composition comprising black truffle and plant exosomes is a fermentation product of Thermus thermophilus and a fermentation product filtrate of Lactobacillus / mung bean seed extract / monosodium glutamate in a mass ratio of 2:1.5, and the addition amount of the fermentation product filtrate is kept unchanged, and the other components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0099] Example 12

[0100] The present embodiment provides a skin care composition comprising black truffle and plant exosomes, wherein the fermentation product filtrate in the skin care composition comprising black truffle and plant exosomes is a lactobacillus / soymilk fermentation product filtrate and a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate in a mass ratio of 1:1, and the addition amount of the fermentation product filtrate is kept unchanged, and the other components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0101] Comparative Example 1

[0102] This comparative example provides a skin care composition comprising black truffle and plant exosomes, wherein the components of the skin care composition comprising black truffle and plant exosomes do not include black truffle extract, and the reduced amount thereof is distributed to Chlorella exosomes, malachite extract, fermentation product filtrate and soluble collagen in proportion to the number of parts, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0103] Comparative Example 2

[0104] This comparative example provides a skin care composition comprising black truffle and plant exosomes, wherein the components of the skin care composition comprising black truffle and plant exosomes do not include Chlorella exosomes, and the reduced amount thereof is distributed to the black truffle extract, the malachite extract, the fermentation product filtrate and the soluble collagen in proportion to the number of parts, and the remaining components and the addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0105] Comparative Example 3

[0106] This comparative example provides a skin care composition comprising black truffle and plant exosomes, wherein the components of the skin care composition comprising black truffle and plant exosomes do not include malachite extract, and the reduced amount thereof is distributed to black truffle extract, Chlorella exosomes, fermentation product filtrate and soluble collagen in proportion to the number of parts, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0107] Comparative Example 4

[0108] This comparative example provides a skin care composition comprising black truffle and plant exosomes, wherein the components of the skin care composition comprising black truffle and plant exosomes do not include fermentation product filtrate, and the reduced amount thereof is distributed to black truffle extract, chlorella exosomes, malachite extract and soluble collagen in proportion to the number of parts, and the remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0109] Comparative Example 5

[0110] This comparative example provides a skin care composition comprising black truffle and plant exosomes, wherein the components of the skin care composition comprising black truffle and plant exosomes do not include soluble collagen, and the reduced amount thereof is distributed to the black truffle extract, the chlorella exosomes, the malachite extract and the fermentation product filtrate in proportion, and the remaining components and the addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0111] Application Example 1

[0112] This application example provides an essence, the formula of which is as follows, and the dosage unit of each component in the table is "part".

[0113]

[0114]

[0115] Its preparation method is:

[0116] (1) stirring water, a humectant, and a thickener, heating the mixture to 70° C., and then maintaining the temperature for 0.5 h to obtain an aqueous phase liquid;

[0117] (2) stirring the oil and emulsifier and heating to 80°C for 1 hour to obtain an oil phase liquid;

[0118] (2) After the heat preservation is completed, the aqueous phase liquid and the oil phase liquid are mixed and homogenized at 70° C., and after cooling to 45° C., the skin care composition containing black truffle and plant exosomes and the essence are added, and the essence is obtained after homogenization.

[0119] Application Example 2-12

[0120] This application example provides 12 kinds of essences. The components of the essences differ from those of Application Example 1 only in that the skin care composition containing black truffle and plant exosomes prepared in Example 1 is replaced by an equal amount of the skin care composition containing black truffle and plant exosomes prepared in Examples 2-12, and other conditions remain unchanged. The preparation method thereof refers to Application Example 1.

[0121] Comparative Application Examples 1-5

[0122] This application example provides 5 kinds of essences. The components of the essences differ from those of Application Example 1 only in that the skin care composition containing black truffle and plant exosomes prepared in Example 1 is replaced by an equal amount of the skin care composition containing black truffle and plant exosomes prepared in Comparative Examples 1-5, and other conditions remain unchanged. The preparation method thereof refers to Application Example 1.

[0123] Test Example 1 Antioxidant Efficacy Study - DPPH Free Radical Scavenging Experiment

[0124] Test samples: compositions provided in Examples 1-12 and Comparative Examples 1-5;

[0125] Test method:

[0126] Accurately weigh 47.7 mg of DPPH reagent, dilute to a 100 ml brown volumetric flask, take 5 ml of it and dilute to a 50 ml brown volumetric flask with anhydrous ethanol to obtain a DPPH test solution (47.7 mg / L). Pipette 2 mL of DPPH test solution respectively, add 0.1 mL of the sample provided in Examples 1-16 and Comparative Examples 1-5 as shown in the table below, and then add an appropriate amount of anhydrous ethanol to make the total mass equal. After fully mixing, let stand in the dark for 30 min, use anhydrous ethanol as a blank control, measure its absorbance at 517 nm, and set vitamin C as a control, and calculate the scavenging rate of each extract for DPPH according to the following formula:

[0127] DPPH removal rate (%) = [1-(As-Ar) / A0] × 100%

[0128] Where: As is the absorbance of the extract and DPPH reaction system; Ar is the absorbance of the extract-anhydrous ethanol; A0 is the absorbance of the blank control anhydrous ethanol.

[0129]

[0130] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. The higher the scavenging rate, the better the antioxidant degree and the better the anti-aging effect. Therefore, the anti-aging effect of the essence can be judged by studying its ability to scavenge DPPH free radicals. Vitamin C is a highly effective antioxidant recognized by the cosmetics industry. This experiment used vitamin C as a control to examine its antioxidant capacity. The test results are shown in Table 1.

[0131] Table 1

[0132] Sample name DPPH scavenging rate of anti-wrinkle composition (%) Vitamin C 93.16 Example 1 91.13 Example 2 89.28 Example 3 90.53 Example 4 80.12 Example 5 81.28 Example 6 79.62 Example 7 83.51 Example 8 84.17 Example 9 84.72 Example 10 81.25 Embodiment 11 81.96 Example 12 90.46 Comparative Example 1 75.83 Comparative Example 2 82.46 Comparative Example 3 73.78 Comparative Example 4 74.61 Comparative Example 5 73.82

[0133] As can be seen from Table 1, the skin care compositions containing black truffle and plant exosomes of Examples 1-3 have better scavenging effects on DPPH, and the scavenging rates are all above 89%, which is close to that of vitamin C, while the scavenging effects of Examples 4-6 and 7-9 on DPPH are reduced, indicating that the addition of superoxide dismutase, sodium DNA and polypeptide to the composition can make the antioxidant effect of the composition better, and the type of polypeptide selection also affects the antioxidant effect; the type of fermentation product filtrate in Examples 10-12 also affects the antioxidant effect of the composition; the scavenging effect of the composition in Comparative Examples 1-5 on DPPH is reduced, indicating that the four components of black truffle extract, Chlorella exosomes, malachite extract, fermentation product filtrate and soluble collagen promote each other in antioxidant and have a synergistic effect.

[0134] Test Example 2 Whitening effect - in vitro tyrosinase inhibition test

[0135] Test samples: compositions provided in Examples 1-12 and Comparative Examples 1-5;

[0136] Test method:

[0137] Prepare 5mM L-tyrosine solution (L-tyrosine can be dissolved with a small amount of hydrochloric acid and then fixed to volume with PBS buffer) and tyrosinase solution (diluted to 100U / mL with deionized water). Use a micropipette to transfer 0.4mL of sample, 1mL of 0.05mol / L phosphate buffer (pH=6.8) to the cuvette, then add 0.8mL of 0.5mmol / L tyrosine solution, and finally add 0.40mL of tyrosinase solution, mix quickly, and measure the growth curve of the optical density value with a wavelength of 475nm over time at a constant temperature of 30℃, and obtain the inhibition rate from the slope of the curve. Measure the inhibition rate of extracts with different concentrations respectively, draw a straight line with the concentration as the horizontal axis and the inhibition rate as the vertical axis, and after obtaining the linear equation, calculate the extract concentration when the inhibition rate is 50%, which is IC50. The results are shown in Table 2:

[0138] Table 2

[0139]

[0140]

[0141] As shown in Table 2, the skin care composition comprising black truffle and plant exosomes of the present invention has a good inhibitory effect on tyrosinase. Among them, the IC50 values ​​of Examples 1-3 are lower than those of Examples 4-9, indicating that the addition of superoxide dismutase, sodium DNA and polypeptide in the composition can better improve the whitening effect; as shown in Examples 10-12, the type of fermentation product filtrate selection also significantly affects the efficacy of the final product; the IC50 value of Example 1 is lower than that of Comparative Examples 1-5, indicating that the black truffle extract, Chlorella exosomes, Malachite extract, fermentation product filtrate and soluble collagen in the composition of the present invention have a synergistic effect in whitening, verifying the rationality of the composition combination.

[0142] Test Example 3 Hyaluronidase In vitro Inhibition Test

[0143] Hyaluronic acid is a component in the tissue matrix that limits the diffusion of water and other extracellular substances. After being hydrolyzed by hyaluronidase, the cells become non-sticky, causing cell degranulation and the exudation of new synthesized media, exerting biological effects and leading to the occurrence of immediate allergic reactions. Hyaluronidase is a participant in allergic reactions and has a strong correlation with allergies. Allergic phenomena can be alleviated and skin can be improved by inhibiting the activity of hyaluronidase.

[0144] Test samples: compositions provided in Examples 1-12 and Comparative Examples 1-5;

[0145] Test method:

[0146] The hyaluronidase inhibition method was used. Take 0.1mL CaCl2 solution (0.25mmol / L) and 0.5mL hyaluronidase solution (100U / mL) and react in a 37℃ water bath for 20min; add 0.5mL sample and keep warm for 20min; then add 0.5mL sodium hyaluronate solution (0.5g / L), react for 30min, then take out and cool for 5min; add 0.1mL sodium hydroxide solution (0.4mol / L) and 0.5mL acetylacetone solution (3.5mL acetylacetone dissolved in 50mL 1.0mol / L sodium carbonate solution), boil in a water bath for 15min, then immediately transfer to an ice water bath for 5min; add 1mL Ehrlich reagent (0.8g p-dimethylaminobenzaldehyde dissolved in 15mL concentrated hydrochloric acid and 15mL anhydrous ethanol), dilute with 3mL anhydrous ethanol, place at 25℃ for 20min to develop color, measure its absorbance at 540nm, and use dipotassium glycyrrhizinate as a control. The hyaluronidase inhibition rate was calculated according to the following formula:

[0147] Hyaluronidase inhibition rate = {[(A-B)-(C-D)] / (AB)}×100%

[0148] In the formula: A is the absorbance of the control solution (using acetate buffer solution instead of the sample solution); B is the absorbance of the control blank solution (using acetate buffer solution instead of the sample solution and enzyme solution); C is the absorbance of the sample solution; D is the absorbance of the sample blank solution (using acetate buffer solution instead of the enzyme solution).

[0149] Using 5% dipotassium glycyrrhizinate as a positive control, the inhibition rate of each group of samples on hyaluronidase was examined. The results are shown in Table 3:

[0150] Table 3

[0151] Sample name Inhibition rate of hyaluronidase (%) 5% Dipotassium Glycyrrhizinate 82.1 Example 1 85.9 Example 2 84.7 Example 3 85.2 Example 4 78.6 Example 5 79.1 Example 6 78.4 Example 7 82.3 Example 8 83.8 Example 9 83.6 Example 10 80.1 Embodiment 11 79.8 Example 12 81.2 Comparative Example 1 76.4 Comparative Example 2 77.8 Comparative Example 3 73.8 Comparative Example 4 76.4 Comparative Example 5 75.1

[0152] As shown in Table 3, the skin care composition comprising black truffle and plant exosomes of the present invention has a good inhibitory effect on hyaluronidase, and the inhibition rate is above 85%. Among them, the inhibition rate of Example 1 is higher than that of Examples 4-9, indicating that the addition of superoxide dismutase, sodium DNA and polypeptide in the composition can significantly affect the anti-allergic effect of the final product, and can make the anti-allergic effect of the composition better, and the type of polypeptide selection also affects this effect; as shown in Examples 10-12, the type of fermentation product filtrate selection also significantly affects the anti-allergic effect of the final product; the inhibition rate of Example 1 is higher than that of Comparative Examples 1-5, indicating that the black truffle extract, Chlorella exosomes, Malachite extract, fermentation product filtrate and soluble collagen in the composition of the present invention have a synergistic effect in terms of anti-allergic effect, which verifies the rationality of the composition combination.

[0153] Test Example 4: Human evaluation test:

[0154] 102 volunteers aged 20-45 were selected and randomly divided into 17 groups, with 6 people in each group, half of them male and half female. The 17 groups of volunteers used the essence prepared in Application Examples 1-12 and Comparative Application Examples 1-5 on their left sides, once in the morning and evening every day, applying 5±0.5g of the product on the face each time, and massaging for 2 minutes, and continued to use it for 30 days. The volunteers scored the skin whitening ability, skin elasticity, and anti-allergic soothing effects of the left and right faces, with the highest score being 5 points and the lowest score being 1 point. The evaluation results are shown in Table 4 (average score):

[0155] Table 4

[0156]

[0157]

[0158] As shown in Table 4, the composition of the present invention can be added into cosmetic products to significantly improve the sensory scores of skin whitening, elasticity, anti-allergy and soothing.

[0159] Test Example 5: Safety Evaluation Experiment

[0160] This project evaluates irritation through the red blood cell hemolysis test. The red blood cell hemolysis test is one of the alternative methods to the rabbit eye irritation test (Draize test). The basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of hemoglobin dissolution and the degree of denaturation. Internationally, the RBC test is mainly used to evaluate the eye irritation of chemicals such as cosmetics and raw materials. The classification standards are shown in the following table.

[0161]

[0162]

[0163] The results of the red blood cell hemolysis experiment on the samples of Examples 1-12 are shown in Table 5, where L / D is the ratio of HD50 (the sample concentration when 50% of the red blood cells are hemolyzed) to DI (protein denaturation index).

[0164] Table 5

[0165] Sample name L / D evaluate Example 1 ﹥100 Non-irritating Example 2 ﹥100 Non-irritating Example 3 ﹥100 Non-irritating Example 4 ﹥100 Non-irritating Example 5 ﹥100 Non-irritating Example 6 ﹥100 Non-irritating Example 7 ﹥100 Non-irritating Example 8 ﹥100 Non-irritating Example 9 ﹥100 Non-irritating Example 10 ﹥100 Non-irritating Embodiment 11 ﹥100 Non-irritating Example 12 ﹥100 Non-irritating

[0166] It can be seen from Table 5 that the skin care composition comprising black truffle and plant exosomes of the present invention is mild and non-irritating, and has high safety in use.

[0167] The applicant declares that the present invention illustrates a skin care composition containing black truffle and plant exosomes and its application through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of the raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0168] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0169] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A skin care composition comprising black truffle and plant exosomes, characterized in that: The skin care composition comprising black truffle and plant exosomes comprises black truffle extract, chlorella exosomes, malachite extract, fermentation product filtrate and soluble collagen; The fermentation product filtrate includes any one of Thermus thermophilus fermentation product, Lactobacillus / soymilk fermentation product filtrate or Lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate or a combination of at least two thereof.

2. The skin care composition comprising black truffle and plant exosomes according to claim 1, characterized in that The skin care composition containing black truffle and plant exosomes includes, by weight, 1-3 parts of black truffle extract, 1-2 parts of chlorella exosomes, 1-2 parts of malachite extract, 5-10 parts of fermentation product filtrate and 3-6 parts of soluble collagen.

3. The skin care composition comprising black truffle and plant exosomes according to claim 1 or 2, characterized in that: The fermentation product filtrate includes a Thermus thermophilus fermentation product, a lactobacillus / soymilk fermentation product filtrate and a lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate.

4. The skin care composition comprising black truffle and plant exosomes according to claim 3, characterized in that The mass ratio of the Thermus thermophilus fermentation product, the lactobacillus / soymilk fermentation product filtrate and the lactobacillus / mung bean seed extract / monosodium glutamate fermentation product filtrate is (1-3):(1-2):(1-2).

5. The skin care composition comprising black truffle and plant exosomes according to any one of claims 1 to 4, characterized in that: The skin care composition comprising black truffle and plant exosomes also comprises superoxide dismutase; Preferably, the skin care composition comprising black truffle and plant exosomes further comprises 0.1-2 parts of superoxide dismutase by weight.

6. The skin care composition comprising black truffle and plant exosomes according to any one of claims 1 to 5, characterized in that: The skin care composition comprising black truffle and plant exosomes also includes sodium DNA; Preferably, the skin care composition comprising black truffle and plant exosomes further comprises 0.1-1 parts of sodium DNA by weight.

7. The skin care composition comprising black truffle and plant exosomes according to any one of claims 1 to 6, characterized in that: The skin care composition comprising black truffle and plant exosomes also comprises a polypeptide; Preferably, the skin care composition comprising black truffle and plant exosomes further comprises 2-4 parts of polypeptides by weight.

8. The skin care composition comprising black truffle and plant exosomes according to claim 7, characterized in that: The polypeptide comprises any one or a combination of at least two of palmitoyl tripeptide-8, tetrapeptide-1 or nonapeptide-1; Preferably, the polypeptides include palmitoyl tripeptide-8, tetrapeptide-1 and nonapeptide-1.

9. The skin care composition comprising black truffle and plant exosomes according to claim 8, characterized in that The mass ratio of the palmitoyl tripeptide-8, tetrapeptide-1 and nonapeptide-1 is (2-4):(1-3):(1-4).

10. Use of the skin care composition comprising black truffle and plant exosomes according to any one of claims 1 to 9 in the preparation of cosmetics.

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