Detection device for changing imaging method through optical system

Abstract

The invention belongs to the technical field of detection instruments, and discloses a detection device for changing an imaging method through an optical system.The detection device is internally provided with a fluorescence excitation module and a fluorescence receiving module, the fluorescence excitation module internally comprises a laser, a cuvette and a light transmission channel, and the optical system in the light transmission channel shapes a fluorescence beam to obtain a fluorescence signal; it is guaranteed that the focus of a fluorescent light beam is hit on the CCD, the focus of fluorescent light of different light transmission channels is hit on different positions of the CCD, equivalently, region division is conducted on the CCD, and different regions correspond to different light transmission channels; the fluorescence receiving module comprises a CCD, reflectors are arranged on the two sides of the CCD, one CCD can detect fluorescence in the three light transmission channels at the same time through cooperation of the reflectors, one CCD can be used for detecting three samples at the same time, and the detection efficiency is improved; the problems that a detection device in the prior art is low in efficiency and cannot detect a plurality of samples at the same time are solved, and the device is suitable for detecting ions in a solution.

Description

Technical Field

[0001] The present invention relates to the technical field of detection equipment, and in particular to a detection device for changing an imaging method through an optical system. Background Art

[0002] The fluorescence method is based on the principle that a substance emits light of a specific wavelength after absorbing light energy. The concentration of a substance is determined by measuring the fluorescence intensity emitted by the sample. The principle of the fluorescence method for determining chromium ions is to determine the concentration of chromium ions by measuring the fluorescence intensity in the sample. The specific principle is: a strong fluorescence signal with a peak value of 469.1nm is emitted under 405nm light. When the sample contains chromium ions, the electron transition orbit of the fluorescent probe will change, causing the excited electrons to have a new orbit to return to the ground state. This process significantly inhibits the electron transition mode of releasing energy in the form of radiation (i.e., the fluorescence emission process).

[0003] The existing technology of measuring chromium ion concentration by fluorescence method can only measure a single sample, and its detection efficiency is low. One CCD receiver can often only detect one sample. If multiple samples need to be detected, it is necessary to wait for the previous sample to be detected or use multiple detection devices, resulting in low efficiency or high cost. Therefore, the present invention proposes a device that can detect multiple samples according to needs, reduce costs and improve detection efficiency. Summary of the invention

[0004] The present invention is intended to provide a detection device that changes the imaging method through an optical system, so as to solve the problem that the detection device in the prior art is low in efficiency and cannot perform multiple sample detections at the same time.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A detection device for changing an imaging method through an optical system comprises a bearing shell, a fluorescence excitation module and a fluorescence receiving module, wherein the fluorescence excitation module comprises a laser fixing seat, a cuvette fixing seat and an optical transmission channel, wherein the laser fixing seat is hollow inside for placing a laser, the cuvette fixing seat is used for placing a cuvette, and the cuvette is used for placing a solution to be tested, and the laser and the optical transmission channel are at the same height; the optical transmission channel comprises a filter, a collecting lens and a shaping lens group, wherein the collecting lens is arranged between the filter and the shaping lens group, the filter is used for filtering laser light and only allowing fluorescence to pass through, the collecting lens is used for reducing fluorescence dissipation, and the shaping lens group is used for shaping the fluorescence beam to ensure fluorescence focusing, and ensure that the fluorescence passing through a specific optical system can hit a specific area corresponding to a CCD, thereby realizing fluorescence detection of a CCD regionalized specific sample;

[0007] A fluorescence receiving module corresponds to the three fluorescence excitation modules. A CCD receiver is arranged at the center of the fluorescence receiving module. The CCD receiver is used to receive fluorescence. A first channel port is arranged at the front side of the CCD receiver. A light transmission channel is placed in the first channel port. A second channel port and a third channel port are arranged on both sides of the CCD receiver. Light transmission channels are arranged in the second channel port and the third channel port. A first reflector is arranged opposite to the second channel port and the third channel port. A second reflector is arranged inside the fluorescence receiving module and on both sides of the first channel port. Through the cooperation of the first reflector and the second reflector, the fluorescence in the light transmission channels in the second channel port and the third channel port is reflected to the CCD receiver.

[0008] A battery is arranged in the carrying shell, and a central processing unit is arranged in the carrying shell. The central processing unit includes a display screen, a single-chip microcomputer, a warning light and an alarm. The single-chip microcomputer is used to control the switch of the laser, the result processing of the CCD receiver and the control of the display screen. The warning light and the alarm are used to provide light and sound prompts after the measurement is completed. The single-chip microcomputer is connected to the battery and the CCD receiver through a wiring groove, and the display screen is used to display the chromium ion concentration in the solution.

[0009] The optical system in the optical transmission channel of the detection device specifically includes: the plastic lens group in the optical transmission channel of the first channel port includes: a first lens, a second lens, a third lens and a fourth lens, the interval between the first lens and the second lens is 0.5mm, the interval between the second lens and the third lens is 14.1mm, the interval between the third lens and the fourth lens is 0.5mm, the center thickness of the first lens is 2.14mm, the radius of curvature of the object side is -29.11mm, and the radius of curvature of the image side is -11.056mm, the center thickness of the second lens is 3.1mm, the radius of curvature of the object side is 54.56mm, and the radius of curvature of the image side is -23.34mm, the center thickness of the third lens is 1mm, the radius of curvature of the object side is -8.96mm, and the radius of curvature of the image side is -16.68mm, the center thickness of the fourth lens is 6.64mm, the radius of curvature of the object side is 13.57mm, and the radius of curvature of the image side is -144.18mm;

[0010] The plastic lens group in the optical transmission channels of the second channel opening and the third channel opening includes: a fifth lens, a sixth lens, a seventh lens and an eighth lens. The interval between the fifth lens and the sixth lens is 0.6 mm, the interval between the sixth lens and the seventh lens is 15.45 mm, the interval between the seventh lens and the eighth lens is 0.58 mm, the center thickness of the fifth lens is 2.36 mm, the radius of curvature of the object side is -22.51 mm, and the radius of curvature of the image side is -11.15 mm, the center thickness of the sixth lens is 3.57 mm, the radius of curvature of the object side is 280.17 mm, and the radius of curvature of the image side is -21.09 mm, the center thickness of the seventh lens is 1 mm, the radius of curvature of the object side is -12.01 mm, and the radius of curvature of the image side is -21.55 mm, the center thickness of the eighth lens is 4.42 mm, the radius of curvature of the image side is 25.87 mm, and the radius of curvature of the object side is -72.09 mm.

[0011] Furthermore, in the device, two fluorescence receiving modules are provided, and six fluorescence excitation modules are correspondingly provided.

[0012] Furthermore, the dimensions of the bearing shell are set to be length*width*height: 240*170*50mm.

[0013] Furthermore, the battery is a rechargeable battery, and a charging port is provided on the outside of the carrying shell.

[0014] Furthermore, the model of the single-chip microcomputer is STM32 single-chip microcomputer.

[0015] Principle and beneficial effects of the technical solution: The fluorescence excitation module of the present invention irradiates the solution with laser, so that the solution emits fluorescence, and the fluorescence is irradiated on the CCD receiver through the optical transmission channel. After processing, the chromium ion concentration is finally displayed on the display screen. At the same time, the present invention can simultaneously transmit the fluorescence light beams in the three optical transmission channels to different positions of the CCD through the cooperation between the reflectors, and obtain different fluorescence intensities by reading the numbers at different positions of the CCD; the present invention can detect three samples using one CCD receiver, which effectively improves efficiency and reduces costs; the filter in the optical transmission channel of the present invention is used to filter the laser, the collection lens is used to reduce fluorescence dissipation, and the shaping lens group is used for fluorescence focusing, so as to improve the fluorescence collection rate and the accuracy of the light beam focusing position, thereby effectively improving the accuracy of the data; the present invention sets 6 optical paths, 3 of which are a unit, and the number of samples detected by the device at a single time can be changed according to needs. The present invention can detect multiple samples at the same time, and the display screen visualizes the results, which is more intuitive; the present invention concentrates the fluorescence of a sample to a fixed position of the CCD receiver through the optical transmission channel, thereby realizing multi-channel detection of the CCD. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is an external structural diagram of the device of the present invention;

[0017] Figure 2 It is the internal structure diagram of the device of the present invention;

[0018] Figure 3 It is a cross-sectional view of the optical transmission channel of the present invention;

[0019] Figure 4 It is a top cross-sectional view of the fluorescence receiving module of the present invention;

[0020] Figure 5 This is the formal diagram of the fluorescence receiving module of the present invention;

[0021] Figure 6 It is a schematic diagram of light beam transmission of the present invention;

[0022] Figure 7 It is a schematic diagram of focusing of the shaping lens group of the light transmission channel in the first channel of the present invention;

[0023] Figure 8 It is a schematic diagram of focusing the shaping lens group of the second and third channel light transmission channels in the present invention;

[0024] Fig. 9 is a point diagram of the light transmission channel in the first channel port of the present invention;

[0025] Fig.10 is a point diagram of the light transmission channel in the second channel port of the present invention;

[0026] Fig.11 The MTF diagram of the optical system in the first channel of the present invention;

[0027] Fig.12 is the MTF diagram of the optical system in the second channel of the present invention;

[0028] The names of corresponding marks in the accompanying drawings are: carrying shell 1, fluorescence excitation module 2, fluorescence receiving module 3, laser 4, cuvette 5, battery 6, central processing unit 7, wiring groove 11, laser fixing seat 21, cuvette fixing seat 22, optical transmission channel 23, CCD receiver 31, first channel opening 32, second channel opening 33, third channel opening 34, first reflector 35, second reflector 36, charging port 61, display screen 71, single-chip microcomputer 72, warning light 73, alarm 74, filter 231, collecting lens 232, shaping lens group 8, first lens 81, second lens 82, third lens 83, fourth lens 84, fifth lens 85, sixth lens 86, seventh lens 87, eighth lens 88. DETAILED DESCRIPTION

[0029] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments:

[0030] like Figure 1-Figure 8 As shown, a detection device for changing an imaging method through an optical system includes a carrying shell 1, a wiring groove 11 is arranged on the left side of the carrying shell 1, and a groove body for placing a battery 6 and a central processing unit 7 are arranged on the upper and lower sides of the carrying shell 1, respectively. The central processing unit 7 includes an STM32 single-chip microcomputer 72, and a display screen 71 is arranged on the side of the STM32 single-chip microcomputer 72. The STM32 single-chip microcomputer 72 is used to control the switch of the laser 4, the result processing of the CCD receiver 31 and the control of the display screen 71. A warning light 73 and an alarm 74 are placed in the side groove body where the battery 6 is placed. The warning light 73 and the alarm 74 The alarm 74 is used to emit sound and light prompts after the sample detection is completed; two fluorescence receiving modules 3 and six fluorescence excitation modules 2 are arranged inside the supporting shell 1, each fluorescence receiving module 3 corresponds to three fluorescence excitation modules 2, and the fluorescence excitation module 2 includes a laser fixing seat 21, a cuvette fixing seat 22 and an optical transmission channel 23. The laser fixing seat 21 is hollow inside for placing the laser 4, and the cuvette fixing seat 22 is used to place and fix the cuvette 5. The optical transmission channel 23 is at the same height as the laser 4, and the optical transmission channel 23, the cuvette 5 and the laser 4 are in the same vertical direction, so that To ensure fluorescence collection, a filter 231, a collection lens 232 and a shaping lens group 8 are arranged in the optical transmission channel 23. The collection lens 232 is arranged between the filter 231 and the shaping lens group 8. The filter 231 is used to filter the laser and only allow fluorescence to pass through. The collection lens 232 can reduce the dissipation of fluorescence. The shaping lens group 8 realizes fluorescence beam shaping to ensure that the fluorescence is focused on the CCD receiver 31 after passing through the lens; the fluorescence receiving module 3 includes a CCD receiver 31, and a first channel opening 32 is arranged on the front side of the CCD receiver 31. A fixed optical transmission channel 2 is placed in the first channel opening 32. 3, the second channel opening 33 and the third channel opening 34 are respectively arranged on the left and right sides of the CCD receiver 31, the light transmission channel 23 is placed in the second channel opening 33 and the third channel opening 34, the first channel opening 32, the second channel opening 33 and the third channel opening 34 are at different heights, a first reflector 35 is arranged opposite to the second channel opening 33 and the third channel opening 34, inside the fluorescence receiving module 3, second reflectors 36 are arranged on both sides of the first channel opening 32, and the first reflector 35 and the second reflector 36 on the same side cooperate with each other to transmit the fluorescence in the light transmission channel 23 to the CCD receiver 31.

[0031] There are two optical systems in the light transmission channel of the detection device, namely, an optical system located in the first channel port without passing through a reflector and an optical system located in the second and third channel ports through a reflector. The shaping lens group in the light transmission channel at the first channel port includes a first lens 81, a second lens 82, a third lens 83 and a fourth lens 84, and the shaping lens group in the light transmission channel at the second and third channel ports includes a fifth lens 85, a sixth lens 86, a seventh lens 87 and an eighth lens 88.

[0032] The lens parameters in the light transmission channel of the first channel are shown in Table 1:

[0033] Table 1 Lens parameters in the light transmission channel of the first channel

[0034]

[0035]

[0036] As shown in Table 1, the interval between the first lens and the object plane is 15 mm, the interval between the first lens and the second lens is 0.5 mm, the interval between the second lens and the third lens is 14.1 mm, the interval between the third lens and the fourth lens is 0.5 mm, the interval between the fourth lens and the image plane is 20 mm, the center thickness of the first lens is 2.14 mm, the radius of curvature of the object side surface is -29.11 mm, and the radius of curvature of the image side surface is -11.056 mm, the center thickness of the second lens is 3.1 mm, the radius of curvature of the object side surface is 54.56 mm, and the radius of curvature of the image side surface is -23.34 mm, the center thickness of the third lens is 1 mm, the radius of curvature of the object side surface is -8.96 mm, and the radius of curvature of the image side surface is -16.68 mm, the center thickness of the fourth lens is 6.64 mm, the radius of curvature of the object side surface is 13.57 mm, and the radius of curvature of the image side surface is -144.18 mm;

[0037] The lens parameters in the light transmission channels at the second channel port and the third channel port are shown in Table 2:

[0038] Table 2 Lens parameters in the light transmission channels of the second and third channel ports

[0039]

[0040]

[0041] As shown in Table 2, the interval between the fifth lens and the object plane is 15 mm, the interval between the fifth lens and the sixth lens is 0.6 mm, the interval between the sixth lens and the seventh lens is 15.45 mm, the interval between the seventh lens and the eighth lens is 0.58 mm, the interval between the eighth lens and the image plane is 50 mm, the center thickness of the fifth lens is 2.36 mm, the radius of curvature of the object side surface is -22.51 mm, and the radius of curvature of the image side surface is -11.15 mm, the center thickness of the sixth lens is 3.57 mm, the radius of curvature of the object side surface is 280.17 mm, and the radius of curvature of the image side surface is -21.09 mm, the center thickness of the seventh lens is 1 mm, the radius of curvature of the object side surface is -12.01 mm, and the radius of curvature of the image side surface is -21.55 mm, the center thickness of the eighth lens is 4.42 mm, the radius of curvature of the image side surface is 25.87 mm, and the radius of curvature of the object side surface is -72.09 mm.

[0042] The specific implementation process is as follows: first, the solution to be tested is placed in the cuvette 5 according to the needs, and then the cuvette 5 is placed on the cuvette fixing seat 22, and the laser 4 is controlled to be turned on by the STM single-chip computer 72 to emit laser light. After the laser light penetrates the cuvette 5 and irradiates the solution, the electrons in the solution absorb energy and transition to an excited state, and the electrons are released in the form of radiated photons, that is, fluorescence is emitted. The fluorescence passes through the optical transmission channel 23, passes through the filter 231, the collecting lens 232, and the shaping lens group 8 in the optical transmission channel 23, and finally irradiates the CCD receiver 31. Since the optical transmission channel 23 in the first channel port 32 is directly facing the CCD receiver 31, the fluorescence is directly irradiated on the CCD receiver 31, and the fluorescence in the second channel port 33 and the third channel port 34 is reflected by the first reflector 35 and the second reflector 36, and finally the fluorescence is irradiated on the CCD receiver 31. The process is as follows Figure 6 As shown, the dotted line is the process of light transmission; after the fluorescence is irradiated on the CCD receiver 31, the fluorescence intensity at different positions on the CCD receiver 31 is measured and processed. After the fluorescence is received on the CCD receiver 31, the warning light 73 lights up, the alarm 74 emits a sound prompt, and finally the measurement result of the ion concentration is displayed on the display screen 71.

[0043] The two optical systems in the detection device of the present invention are simulated, and the fluorescence wavelength (λ) used in the simulation wavelength is 469.1 nm. Fig. 9 As shown in FIG. 1 , it is a point diagram of the light transmission channel in the first channel port of the detection device of the present invention; Fig.10 As shown, it is a point diagram of the light transmission channel in the second channel port of the detection device of the present invention; Fig. 9 It can be seen from the figure that the RMS radius in the spot diagram is 0.002λ and the GEO radius is 0.003λ; Fig.10It can be seen that the RMS radius in the point diagram is 0.004λ, and the GEO radius is 0.006λ. The smaller the corresponding values ​​are, the better the imaging quality (beam shaping and focusing effects). It can be seen that the optical system in the detection device of the present invention has good imaging quality.

[0044] like Fig.11 The figure shows the MTF diagram of the optical system in the light transmission channel of the first channel port. Fig.12 Shown is the MTF diagram of the optical system in the light transmission channel of the second channel port. In the MTF diagram, the horizontal axis represents different spatial frequencies and the vertical axis represents the modulation degree. It can be seen from the figure that the change trend of the MTF curve is relatively flat, and it can ensure a high modulation degree in a wide range of spatial frequencies. The slope is small, indicating that the effect aberration and distortion after lens imaging are small, and the imaging resolution is high.

[0045] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the technical solution of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A detection device for changing the imaging method through an optical system, characterized in that: The invention comprises a bearing shell (1), a fluorescence excitation module (2) and a fluorescence receiving module (3); the fluorescence excitation module (2) comprises a laser fixing seat (21), a cuvette fixing seat (22) and a light transmission channel (23); the laser fixing seat (21) is hollow inside and is used to place a laser (4); the cuvette fixing seat (22) is used to place a cuvette (5); the cuvette (5) is used to place a solution to be measured; the laser (4) and the light transmission channel (23) are at the same height; the light transmission channel (23) comprises a filter (231), a collecting lens (232) and a shaping lens group (8); the collecting lens (232) is arranged between the filter (231) and the shaping lens group (8); the filter (231) is used to filter laser light and only allow fluorescence to pass through; the collecting lens (232) is used to reduce fluorescence dissipation; and the shaping lens group (8) is used to shape the fluorescence beam to ensure fluorescence focusing; The fluorescence receiving module (3) corresponds to the three fluorescence excitation modules (2). A CCD receiver (31) is arranged at the center of the fluorescence receiving module (3). The CCD receiver (31) is used to receive fluorescence. A first channel opening (32) is arranged at the front side of the CCD receiver (31). A light transmission channel (23) is placed in the first channel opening (32). A second channel opening (33) and a third channel opening (34) are arranged on both sides of the CCD receiver (31). The first channel opening (32), the second channel opening (33) and the third channel opening (34) are located at different positions. height, a light transmission channel (23) is provided in the second channel opening (33) and the third channel opening (34), a first reflector (35) is provided opposite to the second channel opening (33) and the third channel opening (34), and a second reflector (36) is provided inside the fluorescence receiving module (3) and on both sides of the first channel opening (32), and through the cooperation of the first reflector (35) and the second reflector (36), the fluorescence in the light transmission channel (23) in the second channel opening (33) and the third channel opening (34) is reflected onto the CCD receiver (31); A battery (6) is arranged in the carrying shell (1), and a central processing unit (7) is arranged in the carrying shell (1). The central processing unit (7) comprises a display screen (71), a single chip computer (72), a warning light (73) and an alarm (74). The single chip computer (72) is used to control the switch of the laser (4), the result processing of the CCD receiver (31) and the control of the display screen (71). The warning light (73) and the alarm (74) are used to complete the light and sound prompts after the measurement. The single chip computer (72) is connected to the battery (6) and the CCD receiver (31) through a wiring groove (11). The display screen (71) is used to display the chromium ion concentration in the solution.

2. The optical system in the detection device for changing the imaging method through the optical system according to claim 1, characterized in that: The plastic lens group (8) in the light transmission channel of the first channel opening (32) comprises: a first lens (81), a second lens (82), a third lens (83) and a fourth lens (84), the interval between the first lens (81) and the second lens (82) is 0.5 mm, the interval between the second lens (82) and the third lens (83) is 14.1 mm, the interval between the third lens (83) and the fourth lens (84) is 0.5 mm, the center thickness of the first lens (81) is 2.14 mm, the curvature of the object side is half The diameter of the lens is -29.11mm, and the radius of curvature of the image side is -11.056mm; the center thickness of the second lens (82) is 3.1mm, the radius of curvature of the object side is 54.56mm, and the radius of curvature of the image side is -23.34mm; the center thickness of the third lens (83) is 1mm, the radius of curvature of the object side is -8.96mm, and the radius of curvature of the image side is -16.68mm; the center thickness of the fourth lens (84) is 6.64mm, the radius of curvature of the object side is 13.57mm, and the radius of curvature of the image side is -144.18mm; The plastic lens group (8) in the light transmission channel of the second channel opening (33) and the third channel opening (34) comprises: a fifth lens (85), a sixth lens (86), a seventh lens (87) and an eighth lens (88), the interval between the fifth lens (85) and the sixth lens (86) is 0.6 mm, the interval between the sixth lens (86) and the seventh lens (87) is 15.45 mm, the interval between the seventh lens (87) and the eighth lens (88) is 0.58 mm, the center thickness of the fifth lens (85) is 2.36 mm, The object side curvature radius is -22.51 mm, and the image side curvature radius is -11.15 mm. The center thickness of the sixth lens (86) is 3.57 mm, the object side curvature radius is 280.17 mm, and the image side curvature radius is -21.09 mm. The center thickness of the seventh lens (87) is 1 mm, the object side curvature radius is -12.01 mm, and the image side curvature radius is -21.55 mm. The center thickness of the eighth lens (88) is 4.42 mm, the image side curvature radius is 25.87 mm, and the object side curvature radius is -72.09 mm.

3. The detection device for changing the imaging method by an optical system according to claim 1, characterized in that: In the device, two fluorescence receiving modules (3) are provided, and correspondingly, six fluorescence excitation modules (2) are provided.

4. The detection device for changing the imaging method by an optical system according to claim 1, characterized in that: The dimensions of the bearing shell (1) are set to be length*width*height: 240*170*50 mm.

5. The detection device for changing the imaging method by an optical system according to claim 1, characterized in that: The battery (6) is a rechargeable battery, and a charging port (61) is provided on the outside of the carrying shell (1).

6. The detection device for changing the imaging method by an optical system according to claim 1, characterized in that: The model of the single chip microcomputer (72) is an STM32 single chip microcomputer.

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