Method for preparing roxburgh rose powder

Abstract

The invention provides a method for preparing rosa roxburghii tratt fruit powder, which comprises the following steps: pretreating rosa roxburghii tratt fruits to obtain a pretreatment solution; performing ultrahigh pressure treatment on the pretreatment solution to obtain a treatment solution; and drying the treating fluid to obtain the roxburgh rose powder. According to the preparation method disclosed by the invention, the roxburgh rose fruit can be prepared into the roxburgh rose powder, the powder yield of the roxburgh rose powder is improved, and meanwhile, the vitamin C content and the SOD enzyme activity in the roxburgh rose powder are improved.

Description

Technical Field

[0001] The present invention relates to the field of food. Specifically, the present invention relates to a method for preparing roxburghii powder. Background Art

[0002] The fruit of the roxburghii is rich in vitamin C, superoxide dismutase, polyphenols and other bioactive substances, and has rich nutritional and medicinal value. Developing it into different products can meet the market's growing demand for healthy food and provide consumers with more diversified choices.

[0003] The development of roxburgh pear powder can effectively retain the nutrients in roxburgh pear, prolong the storage time, and can be widely used as a raw material in food, health products, cosmetics and other industries to enhance the added value of roxburgh pear industry. However, the powder yield of roxburgh pear during spray drying is low and the loss of nutrients is serious. Therefore, improving its powder yield is crucial for the roxburgh pear industry. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems existing in the prior art to a certain extent. To this end, the present invention provides a method for preparing roxburghii powder, which significantly improves the powder yield of roxburghii powder by the method of the present invention, effectively retains active substances such as SOD and vitamin C in roxburghii, improves the nutritional value of the product, enhances the market competitiveness of the roxburghii industry, meets the market demand for healthy food, is environmentally friendly, and has important economic and social value.

[0005] In one aspect of the present invention, the present invention provides a method for preparing roxburghii powder. According to an embodiment of the present invention, the method comprises: pre-treating roxburghii fruit to obtain a pre-treatment liquid; subjecting the pre-treatment liquid to ultra-high pressure treatment to obtain a treatment liquid; and drying the treatment liquid to obtain roxburghii powder.

[0006] According to an embodiment of the present invention, the pressure of the ultra-high pressure treatment is 100-500 MPa, and the time is 10-20 min.

[0007] According to an embodiment of the present invention, the ultra-high pressure treatment is carried out in an inert gas environment, wherein the inert gas is one of carbon dioxide, argon, helium and nitrogen; and the inflated amount of the inert gas is 10% to 30% based on the volume of the pretreatment liquid. Thus, the powder extraction rate of the roxburghii powder can be increased, and the vitamin C content and SOD enzyme activity in the roxburghii powder can be increased.

[0008] According to the method for preparing roxburghii powder of the present invention, roxburghii fruit is first pretreated, and a liquid part containing rich nutrients and bioactive substances is extracted from roxburghii fruit. Then, by ultra-high pressure treatment, inert gas can be used to prevent the treatment liquid from being oxidized during the ultra-high pressure treatment process, protect the active ingredients therein from being destroyed, and avoid the growth of bacteria and microorganisms, thereby ensuring the safety and stability of the product; ultra-high pressure can change the interaction between molecules in the liquid at the same time, promote the formation of a tighter combination between molecules, which helps to form a more uniform and stable powder structure during the spray drying process, thereby improving the powder yield, and ultra-high pressure treatment can inhibit certain chemical reactions that cause the destruction of active ingredients to a certain extent, such as oxidation reaction and heat-sensitive reaction, thereby protecting the active substances in roxburghii. Finally, drying treatment is carried out to remove the moisture in the treatment liquid to obtain a dry powder product. Thus, the powder yield is improved and the vitamin C content and SOD enzyme activity in the roxburghii powder are improved.

[0009] According to an embodiment of the present invention, the method for preparing roxburghii powder may also have the following additional features:

[0010] According to an embodiment of the present invention, the pretreatment includes: crushing the roxburghii fruit and performing solid-liquid separation to collect the supernatant; concentrating the supernatant to obtain a roxburghii concentrate; and mixing the roxburghii concentrate with a protective agent to obtain the pretreatment solution.

[0011] According to an embodiment of the present invention, the protective agent includes dextrin; according to an embodiment of the present invention, the dextrin includes β-cyclodextrin and / or maltodextrin; based on the volume of the roxburghii concentrate, the added amount of dextrin is 2 to 20% by volume, preferably 3 to 10% by volume. Thus, the powder extraction rate, the vitamin C content and the SOD enzyme activity in the roxburghii powder can be further improved.

[0012] According to an embodiment of the present invention, the solid-liquid separation process includes centrifugal treatment; the rotation speed of the centrifugal treatment is 3000-7000g, and the time is 5-15min.

[0013] According to an embodiment of the present invention, the concentration treatment comprises an ultrafiltration concentration treatment, the molecular weight cut-off of the ultrafiltration concentration treatment is 1 to 20 Kda, and the transmembrane pressure is 0.1 to 1 MPa.

[0014] According to an embodiment of the present invention, the drying process comprises a spray drying process. Thus, efficient conversion of roxburghii roxburghii juice into roxburghii roxburghii powder with high active ingredients is achieved.

[0015] In another aspect of the present invention, the present invention proposes a method for preparing roxburghii powder. According to an embodiment of the present invention, the method comprises: after the roxburghii fruit is crushed, centrifuged at 5000g for 10min, and the supernatant is collected; the supernatant is subjected to ultrafiltration and concentration treatment under the conditions of a molecular weight cutoff of 10KDa and a transmembrane pressure of 0.5MPa to obtain a roxburghii concentrate; the roxburghii concentrate is mixed with dextrin to obtain a pre-treated liquid; under an inert gas environment, 20% of the volume of the pre-treated liquid is charged into the pre-treated liquid, and the pre-treated liquid is treated at 300MPa for 15min to obtain a treated liquid; the treated liquid is spray-dried at an inlet air temperature of 135°C and a feed flow rate of 5mL / min to obtain roxburghii powder; the paste is selected from β-cyclodextrin and / or maltodextrin; based on the volume of the roxburghii concentrate, the amount of dextrin added is 5% by volume. The inventors obtained the above-mentioned preferred conditions through a large number of experiments, which further improved the powder extraction rate of roxburghii powder and increased the vitamin C content and SOD enzyme activity in the roxburghii powder.

[0016] It should be noted that the characteristics and advantages described above for the method for preparing roxburghii powder are also applicable to this method and will not be repeated here.

[0017] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0019] Figure 1 This is a graph showing the powder yield of roxburghii powder in Test Example 1 of the present invention;

[0020] Figure 2 This is a graph showing the results of investigating the SOD activity of roxburghii powder in Test Example 2 of the present invention;

[0021] Figure 3 This is a graph showing the results of investigating the vitamin C content of roxburghii powder in Test Example 2 of the present invention. DETAILED DESCRIPTION

[0022] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be understood as limiting the present invention.

[0023] It should be noted that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. Further, in the description of the present invention, unless otherwise specified, the meaning of "plurality" is two or more.

[0024] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0025] In this document, the terms “include” or “comprising” are open expressions, that is, including the contents specified in the present invention but not excluding other contents.

[0026] As used herein, the terms "optionally", "optional" or "optionally" generally mean that the subsequently described event or circumstance may but need not occur, and that the description includes instances where the event or circumstance occurs and instances where it does not.

[0027] The present invention proposes a method for preparing roxburghii powder. According to an embodiment of the present invention, the method comprises: pre-treating roxburghii fruit to obtain a pre-treatment liquid; subjecting the pre-treatment liquid to ultra-high pressure treatment to obtain a treatment liquid; and drying the treatment liquid to obtain roxburghii powder. The inventors obtained the above-mentioned optimal conditions through a large number of experiments, thereby increasing the powder extraction rate of roxburghii powder and the content of active ingredients. If the ultra-high pressure treatment pressure is too low or the time is too short, it is easy to cause a decrease in the powder extraction rate and a loss of active substances. If the ultra-high pressure treatment pressure is too high or the time is too long, it may cause the destruction of active substances and the denaturation of active substances.

[0028] According to an embodiment of the present invention, the pressure of the ultra-high pressure treatment is 100-500 MPa, and the time is 10-20 min. Thus, the powder extraction rate of the roxburghii powder is improved, and the vitamin C content and SOD enzyme activity in the roxburghii powder are improved.

[0029] According to an embodiment of the present invention, the ultra-high pressure treatment is carried out in an inert gas environment, wherein the inert gas is one of carbon dioxide, argon, helium and nitrogen; and the inert gas filling amount is 10% to 30% based on the volume of the pretreatment liquid. Thus, the quality and stability of the roxburghii powder are improved.

[0030] According to an embodiment of the present invention, the pretreatment comprises: crushing the roxburghii fruit and performing solid-liquid separation, collecting the supernatant; concentrating the supernatant to obtain a roxburghii concentrate; and mixing the roxburghii concentrate with a protective agent to obtain the pretreatment solution. Thus, the active ingredients in the roxburghii are extracted and enriched, laying a foundation for subsequent ultra-high pressure treatment and spray drying.

[0031] According to an embodiment of the present invention, the protective agent includes dextrin.

[0032] According to an embodiment of the present invention, the dextrin includes β-cyclodextrin and / or maltodextrin, and the added amount of the dextrin is 2 to 20% by volume, preferably 3 to 10% by volume, based on the volume of the roxburghii concentrate, thereby further improving the powder extraction rate and active substance content of the roxburghii powder.

[0033] According to an embodiment of the present invention, the solid-liquid separation process includes centrifugation; the rotation speed of the centrifugation process is 3000-7000g, and the time is 5-15min. Thus, a supernatant rich in active substances is obtained.

[0034] According to an embodiment of the present invention, the concentration process includes ultrafiltration concentration process, and the molecular weight cut-off of the ultrafiltration concentration process is 1 to 20Kda, for example, 1Kda, 2Kda, 3Kda, 4Kda, 5Kda, 6Kda, 7Kda, 8Kda, 9Kda, 10Kda, 11Kda, 12Kda, 13Kda, 14Kda, 15Kda, 16Kda, 17Kda, 18Kda, 19Kda, 20Kda; the transmembrane pressure is 0.1 to 1MPa, for example, 0.1MPa, 0.2MPa, 0.3MPa, 0.4MPa, 0.5MPa, 0.6MPa, 0.7MPa, 0.8MPa, 0.9MPa, 1MPa. Thus, the concentration of active ingredients in the roxburghii concentrate is effectively increased, and small molecule impurities are removed at the same time.

[0035] According to an embodiment of the present invention, the drying process includes spray drying, thereby rapidly removing the water in the roxburghii concentrate to obtain roxburghii powder with a high active substance content.

[0036] In addition, the present invention also proposes a method for preparing roxburghii powder. According to an embodiment of the present invention, the method comprises: after crushing roxburghii fruit, centrifuging at 5000g for 10 minutes, collecting the supernatant; ultrafiltration and concentration of the supernatant under the conditions of a molecular weight cutoff of 10KDa and a transmembrane pressure of 0.5MPa to obtain a roxburghii concentrate; mixing the roxburghii concentrate with dextrin to obtain a pre-treated liquid; in an inert gas environment, charging the pre-treated liquid with nitrogen gas of 20% of the volume of the pre-treated liquid, treating at 300MPa for 15 minutes to obtain a treated liquid; spray drying the treated liquid at an inlet air temperature of 135°C and a feed flow rate of 5mL / min to obtain roxburghii powder; the dextrin is selected from β-cyclodextrin and / or maltodextrin; based on the volume of the roxburghii concentrate, the amount of dextrin added is 5% by volume. The inventors obtained the above-mentioned preferred conditions through a large number of experiments. Therefore, the method of the present invention can improve the powder extraction rate of roxburghii powder, and the prepared roxburghii powder has a higher vitamin C content and a higher SOD enzyme activity.

[0037] The scheme of the present invention will be explained below in conjunction with the embodiments. It will be appreciated by those skilled in the art that the following embodiments are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. Where specific techniques or conditions are not indicated in the embodiments, the techniques or conditions described in the literature in this area or the product specifications are used. The reagents or instruments used are not indicated by the manufacturer and are all conventional products that can be obtained commercially.

[0038] Embodiment 1:

[0039] 1. Select fresh sea buckthorn fruit, wash it, use a spiral juicer to squeeze the juice, filter it, centrifuge it at 5000g for 10 minutes, and take the supernatant.

[0040] 2. The supernatant was ultrafiltered, the ultrafiltration membrane was 10 kDa, the transmembrane pressure was 0.5 MPa, and the intercepted liquid was the roxburghii juice concentrate.

[0041] 3. Add 5% by volume of β-cyclodextrin to the Rosa roxburghii concentrate, continuously fill with 20% by volume of nitrogen, and stir evenly to obtain a first treated solution.

[0042] 4. Place the first treatment liquid into an ultra-high pressure container with a pressure parameter of 300 MPa and a treatment time of 15 min to obtain a second treatment liquid.

[0043] 5. Put the second treated liquid into the injection cup of the spray dryer, set the parameters as inlet air temperature of 135°C and feed flow rate of 5 mL / min, and obtain high-activity roxburghii powder.

[0044] Embodiment 2:

[0045] 1. Select fresh sea buckthorn fruit, wash it, use a spiral juicer to squeeze the juice, filter it, centrifuge it at 3000g for 5 minutes, and take the supernatant.

[0046] 2. The supernatant was ultrafiltered, the ultrafiltration membrane was 1 kDa, the transmembrane pressure was 0.1 MPa, and the intercepted liquid was the roxburghii juice concentrate.

[0047] 3. Add 2% by volume of β-cyclodextrin to the roxburghii concentrate, continuously fill with 10% by volume of nitrogen, and stir evenly to obtain a first treated solution.

[0048] 4. Place the first treatment liquid into an ultra-high pressure container with a pressure parameter of 100 MPa and a treatment time of 10 min to obtain a second treatment liquid.

[0049] 5. Put the second treated liquid into the injection cup of the spray dryer, set the parameters as inlet air temperature of 135°C and feed flow rate of 5 mL / min, and obtain high-activity roxburghii powder.

[0050] Embodiment 3:

[0051] 1. Select fresh sea buckthorn fruit, wash it, use a spiral juicer to squeeze the juice, filter it, centrifuge it at 7000g for 15 minutes, and take the supernatant.

[0052] 2. The supernatant is ultrafiltered, the ultrafiltration membrane is 20 kDa, the transmembrane pressure is 1 MPa, and the intercepted liquid is the roxburghii juice concentrate.

[0053] 3. Add 20% by volume of β-cyclodextrin to the roxburghii concentrate, continuously fill with 30% by volume of nitrogen, and stir evenly to obtain a first treated solution.

[0054] 4. Place the first treatment liquid into an ultra-high pressure container with a pressure parameter of 500 MPa and a treatment time of 20 min to obtain a second treatment liquid.

[0055] 5. Put the second treated liquid into the injection cup of the spray dryer, set the parameters as inlet air temperature of 135°C and feed flow rate of 5 mL / min, and obtain high-activity roxburghii powder.

[0056] Comparative Example 1:

[0057] The difference between Comparative Example 1 and Example 1 is that the ultrahigh pressure treatment in step 4 is not performed, and the first treatment liquid obtained in step 3 is directly subjected to the spray drying treatment in step 5.

[0058] Test example:

[0059] 1. Effect of ultrahigh pressure on the yield of roxburghii powder

[0060] The yields of the roxburghii powders obtained in Examples 1 to 3 and Comparative Example 1 were investigated to evaluate the effect of ultrahigh pressure on the yield of the roxburghii powder.

[0061] The results are as follows Figure 1 As shown, the results show that the powder yield of the roxburghii powder treated with ultra-high pressure is significantly higher than that of the roxburghii powder not treated with ultra-high pressure, and the powder yield of the roxburghii powder prepared in Example 1 is the highest (79%) among all groups, while the powder yield of the roxburghii powder in Comparative Example 1 is the lowest (52%) among all groups.

[0062] 2. Effect of ultrahigh pressure on active substances in roxburghii powder

[0063] The active substances of the roxburghii powder prepared in Examples 1 to 3 and Comparative Example 1 were investigated to evaluate the effect of ultrahigh pressure on the active substances of the roxburghii powder.

[0064] The results are as follows Figures 2-3 As shown, the results show that the SOD activity in the roxburghii powder treated with ultra-high pressure is significantly higher than that in the roxburghii powder not treated with ultra-high pressure, and the SOD activity in the roxburghii powder prepared in Example 1 is the highest among all groups (750 U / mg), while the SOD activity in the roxburghii powder of Comparative Example 1 is the lowest among all groups (540 U / mg); the vitamin C content in the roxburghii powder treated with ultra-high pressure is significantly higher than that in the roxburghii powder not treated with ultra-high pressure, and the vitamin C content in the roxburghii powder prepared in Example 1 is the highest among all groups (790 mg / 100 g), while the vitamin C content in the roxburghii powder of Comparative Example 1 is the lowest among all groups (650 mg / 100 g).

[0065] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0066] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A method for preparing roxburghii powder, characterized in that: include: Pre-treating roxburghii fruit to obtain a pre-treated liquid; The pre-treated liquid is subjected to ultra-high pressure treatment to obtain a treated liquid; The treated liquid is dried to obtain roxburghii powder.

2. The method according to claim 1, characterized in that The ultra-high pressure treatment is performed under a pressure of 100 to 500 MPa and lasts for 10 to 20 minutes.

3. The method according to claim 1 or 2, characterized in that: The ultra-high pressure treatment is carried out in an inert gas environment, wherein the inert gas is one of carbon dioxide, argon, helium and nitrogen; Based on the volume of the pretreatment liquid, the filling amount of the inert gas is 10% to 30%.

4. The method according to claim 1, characterized in that: The pre-processing comprises: The roxburghii fruit is crushed and solid-liquid separated, and the supernatant is collected; The supernatant is concentrated to obtain a roxburghii concentrate; The roxburghii concentrated solution is mixed with a protective agent to obtain the pretreatment solution.

5. The method according to claim 4, characterized in that The protective agent includes dextrin.

6. The method according to claim 5, characterized in that The dextrin includes β-cyclodextrin and / or maltodextrin; Based on the volume of the roxburghii concentrate, the added amount of the dextrin is 2 to 20 volume %.

7. The method according to claim 4, characterized in that The solid-liquid separation process includes centrifugal treatment; The rotation speed of the centrifugal treatment is 3000-7000g, and the time is 5-15min.

8. The method according to claim 4, characterized in that The concentration treatment comprises an ultrafiltration concentration treatment, wherein the cut-off molecular weight of the ultrafiltration concentration treatment is 1 to 20 Kda and the transmembrane pressure is 0.1 to 1 MPa.

9. The method according to claim 1, characterized in that: The drying process includes a spray drying process.

10. A method for preparing roxburghii powder, characterized in that: The method comprises: After the roxburghii fruit was crushed, it was centrifuged at 5000 g for 10 min and the supernatant was collected; The supernatant is subjected to ultrafiltration concentration treatment under the conditions of a molecular weight cut-off of 10 KDa and a transmembrane pressure of 0.5 MPa to obtain a roxburghii concentrate; The roxburghii concentrate is mixed with dextrin to obtain a pretreatment solution; In an inert gas environment, nitrogen gas accounting for 20% of the volume of the pretreatment liquid is charged into the pretreatment liquid, and the pretreatment liquid is treated at 300 MPa for 15 minutes to obtain a treatment liquid; The treated liquid is spray-dried at an inlet air temperature of 135° C. and a feed flow rate of 5 mL / min to obtain roxburghii powder; The paste is selected from β-cyclodextrin and / or maltodextrin; Based on the volume of the roxburghii concentrate, the added amount of the dextrin is 5 volume %.

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