Natural fruit seed oil composition with high antioxidant activity as well as preparation method and application thereof

Abstract

The invention discloses a natural fruit seed oil composition with high antioxidant activity as well as a preparation method and application thereof, and relates to the field of cosmetics. The composition is obtained by extracting, filtering and centrifuging traditional Chinese medicine micro powder and a grease composition, the grease composition comprises simmondsia chinensis seed oil, raspberry seed oil, vaccinium macrocarpum seed oil, grape seed oil, coffee seed oil, rosa canina fruit oil, pomegranate seed oil and European acid cherry seed oil. The natural fruit seed oil is adopted to extract antioxidant active components in astragalus membranaceus and liquorice, so that the influence of an extraction solvent on the antioxidant activity of the extract is effectively avoided, and the dissolution and stability of the antioxidant active components are improved; the natural fruit seed oil is added to accelerate cell rupture and dissolution of a large amount of antioxidant active components, a micromicelle stable system is formed, meanwhile, the natural fruit seed oil has the moisturizing, repairing, anti-inflammatory and antioxidant effects, the antioxidant activity of the product is integrally improved, the antioxidant stability is good, and the storage time is prolonged.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a natural fruit seed oil composition with high antioxidant activity, a preparation method and application thereof. Background Art

[0002] With the development of urbanization, air pollution has intensified, which has a great impact on the health of human skin. In addition, human skin is often exposed to electronic appliances or ultraviolet rays from the sun. Long-term exposure can easily produce a large number of free radicals such as nitrogen, sulfur, and oxygen, causing damage to cells, proteins and other structures. The skin also shows signs of premature aging such as dullness, sagging, sensitivity, and wrinkles. The demand for skin antioxidants is gradually increasing, and cosmetics with high antioxidant activity are also being continuously developed and researched.

[0003] Natural plants have a well-established self-defense system that protects them from oxidative stress caused by many oxygen free radicals including peroxide free radicals. Therefore, some natural plants contain a large number of safe antioxidant active ingredients. Currently, the main methods for extracting antioxidant active ingredients are solvent extraction methods. Solvent extraction methods are to extract effective ingredients from solid raw materials with solvents. The solvents used must have the property of being miscible with the extracted solutes. The method is to crush the plant material, put it into a suitable container, add several times the amount of solvent, and extract it by immersion, percolation, decoction, reflux and continuous extraction.

[0004] However, most of the solvents currently used in extraction are flammable, and the use of large amounts of solvents can lead to the risk of fire. After solvent extraction, the solvent needs to be separated and processed, which is a cumbersome process, and trace amounts of solvent remain in the extract, which may change the antioxidant quality of the product. At the same time, the current solvent extraction lasts for a long time, consumes a lot of energy in production, and produces a large amount of solvent waste, which is not environmentally friendly. In addition, some natural plant antioxidant active ingredients are prone to loss of antioxidant activity during the extraction process and have poor antioxidant stability after extraction, resulting in low content of antioxidant active ingredients. Summary of the invention

[0005] The invention provides a natural fruit seed oil composition with high antioxidant activity, a preparation method and application thereof, and uses natural fruit seed oil to extract antioxidant active substances in astragalus and licorice, thereby effectively improving the antioxidant activity and stability of the composition.

[0006] In order to solve the above technical problems, one of the purposes of the present invention is to provide a natural fruit seed oil composition with high antioxidant activity, wherein the natural fruit seed oil composition is obtained by extracting, filtering and centrifuging 15wt%-30wt% of Chinese medicine micropowder and the rest of the oil composition; the oil composition comprises 30wt%-60wt% of jojoba (SIMMONDSIA CHINENSIS) seed oil and 20wt%-65wt% of fruit seed oil, which account for 30wt%-60wt% of the mass fraction of the natural fruit seed oil composition;

[0007] The fruit seed oil comprises raspberry (RUBUS IDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, small-fruit coffee (COFFEA ARABICA) seed oil, dog rose (ROSACANINA) fruit oil, pomegranate (PUNICA GRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in a mass ratio of (2.5-5.5): (2-5.5): (2-5): (1-5.5): (2.5-5.8): (3-5.5): (3-5.5).

[0008] By adopting the above scheme, the composite natural fruit seed oil added in the present application is rich in active ingredients such as palmitic acid, oleic acid, linoleic acid, linolenic acid, tocopherol, vitamins and phytosterols, has anti-inflammatory and antioxidant effects, and has a high ability to scavenge DPPH free radicals. At the same time, linoleic acid and linoleic acid have a positive effect on skin nutrition and moisturizing, and phytosterols have soothing and anti-inflammatory effects. When used in cosmetics, it can provide soothing, repairing, anti-inflammatory, moisturizing and antioxidant effects. Natural fruit seed oil is used to extract the antioxidant active ingredients in astragalus and licorice. The oil molecules of natural plant oils contain polar functional groups, and some natural plant oils also carry weak polar substances. These polar functional groups are combined with the antioxidant molecules in the astragalus and licorice extracts. The fatty acids in the fruit seed oil can improve the dissolution of the antioxidant active ingredients and increase the content of the antioxidant active ingredients in the composition. The polyphenols, tocopherol and other natural ingredients contained in the natural plant oils are beneficial to improving the antioxidant properties and stability of the oils and antioxidant active molecules. The extracted fruit seed oil does not need to be separated, which can avoid the influence of the current extraction solvent on the antioxidant activity of the extract, ensure the ability of the composition to scavenge free radicals, and have good antioxidant stability. The composition can still ensure high antioxidant activity when stored in a high temperature environment for a long time.

[0009] As a preferred embodiment, the Chinese medicine powder comprises astragalus and licorice in a mass ratio of (1-2):(1-3).

[0010] As a preferred embodiment, the Chinese medicine micropowder includes astragalus and licorice in a mass ratio of 1:1.

[0011] By adopting the above scheme, the present application efficiently extracts the antioxidant active substances of calycosin isoflavones in Astragalus, which have the effects of resisting oxidative damage, anti-radiation and anti-cancer. At the same time, the antioxidant glabridin in licorice is efficiently extracted. Glabridin has anti-inflammatory, anti-allergic and antioxidant effects. The interaction between the natural active ingredients of fruit seed oil, Astragalus and licorice is utilized to promote the coordinated enhancement of their antioxidant activity, thereby maximizing the DPPH free radical scavenging ability.

[0012] As a preferred embodiment, the fruit seed oil includes raspberry (RUBUS IDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, small-fruit coffee (COFFEA ARABICA) seed oil, dog rose (ROSACANINA) fruit oil, pomegranate (PUNICAGRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in a mass ratio of (5-5.5): (2-3): (2-3): (1-2): (5-5.5): (5-5.5): (5-5.5).

[0013] As a preferred embodiment, the fruit seed oil includes raspberry (RUBUS IDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, small-fruit coffee (COFFEA ARABICA) seed oil, dog rose (ROSACANINA) fruit oil, pomegranate (PUNICAGRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in a mass ratio of 5.2:2.5:2.5:1.2:5.2:5.2:5.2.

[0014] By adopting the above scheme, the raspberry (RUBUS IDAEUS) seed oil added in the present application is the one with the highest tocopherol content among vegetable oils, and has excellent antioxidant activity. At the same time, more than 60% of essential fatty acids are added to the high content of tocopherol, which is crucial for skin hydration, inflammation regulation and nutrition; large-fruited blueberry (VACCINIUMMACROCARPON) seed oil can achieve a balance of fatty acids ω3 / ω6ω9, and has antioxidant, moisturizing, soothing and regenerative effects; grape (VITIS VINIFERA) seed oil contains a large amount of linoleic acid and phytosterols, which has a positive effect on skin moisturizing, nutrition and anti-inflammatory; small-fruited coffee (COFFEAARABICA) seed oil is rich in linoleic acid and presents a high unsaponifiable fraction, and has moisturizing, antioxidant and repair effects; dog rose (ROSA Canina fruit oil is rich in essential fatty acids, achieving a balance of ω3 / ω6ω9, and contains a considerable amount of lipophilic antioxidants such as tocopherols and carotenoids. It also contains phenolic acids, which have a high protective effect on inflammation and oxidative stress, and a strong effect on skin repair; pomegranate (PUNICAGRANATUM) seed oil is rich in punicic acid, has lipoxygenase inhibition and anti-inflammatory effects, and can coordinate with phytosterols to improve repair and anti-erythema; European sour cherry (PRUNUS CERASUS) seed oil is very rich in oleic acid, linoleic acid and eleostearic acid, and has moisturizing, antioxidant and nourishing effects. The present application blends seven natural oils in the fruit seed oil, and the rich fatty acids in the fruit seed oil can improve the solubility and stability of the antioxidant active ingredients of astragalus and licorice, thereby improving the antioxidant activity of the composition.

[0015] As a preferred embodiment, the oil composition comprises 43wt%-54wt% of jojoba (SIMMONDSIA CHINENSIS) seed oil and 27wt%-37wt% of fruit seed oil.

[0016] In order to solve the above technical problems, the second object of the present invention is to provide a method for preparing a natural fruit seed oil composition with high antioxidant activity, comprising the following steps:

[0017] (1) drying the astragalus and licorice to a moisture content of less than 20%, and then grinding the astragalus and licorice into Chinese medicine fine powder at a temperature ranging from -80°C to -50°C;

[0018] (2) impregnating and mixing the Chinese medicine powder with the oil composition to obtain a mixture;

[0019] (3) adding the mixture into an extraction device, and performing microwave and ultrasonic extraction alternately under nitrogen conditions to obtain an extract;

[0020] (4) The extract is subjected to microfiltration and then centrifugation to obtain a natural fruit seed oil composition.

[0021] By adopting the above scheme, the present application performs microwave and ultrasonic alternating extraction on the oil composition containing Chinese medicine micropowder. Microwaves can act on cells, causing the internal capacity of cells to expand and generate external stress, bursting the cells from the inside to the outside. Ultrasonic waves act on the outer wall of cells, causing local vacuum to form internal stress, squeezing and breaking the wall from the outside to the inside, which can accelerate cell rupture and large-scale dissolution of antioxidant active ingredients. Microwave and ultrasonic treatment do not affect the biological activity of antioxidant substances in a low-temperature environment. After treatment, non-polar biological molecules and polar biological molecules form a micro-micelle stabilization system, which can improve the biological activity stability of antioxidant substances in the composition, avoid inactivation, and extend the storage time.

[0022] As a preferred embodiment, in step (1), the particle size of the Chinese medicine micropowder after grinding is less than 100 μm.

[0023] As a preferred embodiment, in step (3), the microwave power is 4kW-6kW, the ultrasonic power is 10kHz-80kHz, the ultrasonic and microwave extraction times are both 4-6 times, and the duration of each time is 1min-15min.

[0024] In order to solve the above technical problems, the third object of the present invention is to provide a natural fruit seed oil composition with high antioxidant activity for use in the preparation of cosmetics.

[0025] In order to solve the above technical problems, the fourth purpose of the present invention provides a cosmetic with high antioxidant activity, comprising the following components in parts by weight: 25-30 parts of isohexadecane, 8-12 parts of squalane, 18-22 parts of isononyl isononanoate, 18-22 parts of macadamia seed oil, 18-22 parts of caprylic capric triglyceride, 0.3-1 part of vitamin E, 0.1-0.5 parts of essence, and 0.1-5 parts of natural fruit seed oil composition.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] 1. The present application adopts natural fruit seed oil to extract the antioxidant active ingredients in astragalus and licorice. The natural fruit seed oil is rich in active ingredients such as palmitic acid, oleic acid, linoleic acid, linolenic acid, tocopherol, vitamins and phytosterols, and can achieve the balance of fatty acids ω3 / ω6 / ω9. The oil molecules of natural plant oils contain polar functional groups, which can be combined with the antioxidant active molecules in astragalus and licorice extracts. The fatty acids in the natural fruit seed oil can improve the solubility of antioxidant active ingredients, thereby improving the overall content of antioxidant active ingredients in the product, and the antioxidant stability of the composition is good.

[0028] 2. The natural fruit seed oil used in this application has anti-inflammatory and antioxidant effects, and has a high ability to scavenge DPPH free radicals. At the same time, linoleic acid and linoleic acid have a positive effect on skin nutrition and moisturizing. Phytosterols have soothing and anti-inflammatory effects. When used in cosmetics, they can provide soothing, repairing, anti-inflammatory, moisturizing and antioxidant effects.

[0029] 3. The present application performs microwave and ultrasonic alternating extraction on an oil composition containing Chinese medicine micropowder. Microwaves burst cells from the inside out, and ultrasonic waves squeeze and break the walls from the outside in, which can accelerate cell rupture and the dissolution of a large amount of antioxidant active ingredients. After treatment, non-polar biological molecules and polar biological molecules form a micro-micelle stabilization system, which can improve the biological activity stability of antioxidant substances in the composition and prolong the storage time. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 : Digital images of a natural fruit seed oil composition with high antioxidant activity in Examples 1-3 of the present invention (left - Example 1; middle - Example 2; right - Example 3). DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Table 1 - Sources of raw materials used in the examples or comparative examples of this application

[0033]

[0034]

[0035] Example 1

[0036] A method for preparing a natural fruit seed oil composition with high antioxidant activity comprises the following steps:

[0037] (1) Astragalus membranaceus and Glycyrrhiza glabra are mixed in a mass ratio of 1:1 and dried to a water content of less than 20%. In this embodiment, the water content is preferably controlled at about 10%, and then the temperature of the grinding equipment is controlled at about -80°C. The Astragalus membranaceus and Glycyrrhiza glabra are ground into Chinese medicine powder in a temperature range of -80°C to -50°C. The particle size of the powder after grinding and sieving is less than 80 μm;

[0038] (2) 18 wt % of Chinese medicine powder is impregnated and mixed with 82 wt % of oil composition to obtain a mixture, wherein the oil composition comprises 54 wt % of jojoba (SIMMONDSIACHINENSIS) seed oil and 28 wt % of fruit seed oil, wherein the fruit seed oil comprises 5.5 wt % of raspberry (RUBUS IDAEUS) seed oil, 3 wt % of vaccinium macrocarpon (VACCINIUMMACROCARPON) seed oil, 2 wt % of grape (VITIS VINIFERA) seed oil, 1 wt % of small-fruit coffee (COFFEAARABICA) seed oil, 5.5 wt % of dog rose (ROSA CANINA) fruit oil, 5.5 wt % of pomegranate (PUNICAGRANATUM) seed oil and 5.5 wt % of European sour cherry (PRUNUS CERASUS) seed oil;

[0039] (3) adding the mixture to an extraction device, and performing microwave and ultrasonic extraction alternately under nitrogen conditions, wherein microwaves act on the cells to rupture the cells from the inside out, and ultrasonic waves act on the cell outer wall to squeeze and rupture the cell wall from the outside in. Microwave and ultrasonic treatment allow non-polar biomolecules to encapsulate polar biomolecules, forming a micelle-stabilized system. The microwave power is 6 kW, the ultrasonic power is 50 kHz, and the ultrasonic and microwave extraction times are both 5 times, each time lasting 15 min, to obtain an extract;

[0040] (4) The extract is subjected to microfiltration with a micropore porosity of less than 5 μm, followed by centrifugation to obtain a clear oil, namely, a natural fruit seed oil composition.

[0041] like Figure 1 As shown in the middle left figure, the obtained natural fruit seed oil composition is a uniform yellow-orange transparent oil, and the smell is a nutty bitter almond smell with a sweet taste.

[0042] Example 2

[0043] A method for preparing a natural fruit seed oil composition with high antioxidant activity comprises the following steps:

[0044] (1) Astragalus membranaceus and Glycyrrhiza glabra are mixed in a mass ratio of 1:1 and dried to a water content of about 5%, and then the temperature of the grinding equipment is controlled at about -80°C, and the Astragalus membranaceus and Glycyrrhiza glabra are ground into Chinese medicine powder in a temperature range of -80°C to -50°C, and the particle size of the powder after grinding is less than 100 μm;

[0045] (2) 20 wt% of Chinese medicine powder is impregnated and mixed with 80 wt% of oil composition to obtain a mixture, wherein the oil composition includes 53 wt% of jojoba (SIMMONDSIA CHINENSIS) seed oil and 27 wt% of fruit seed oil, and the fruit seed oil includes 5.2 wt% of raspberry (RUBUS IDAEUS) seed oil, 2.5 wt% of vaccinium macrocarpon (VACCINIUM MACROCARPON) seed oil, 2.5 wt% of grape (VITIS VINIFERA) seed oil, 1.2 wt% of small-fruit coffee (COFFEAARABICA) seed oil, 5.2 wt% of dog rose (ROSACANINA) fruit oil, 5.2 wt% of pomegranate (PUNICAGRANATUM) seed oil and 5.2 wt% of European sour cherry (PRUNUS CERASUS) seed oil;

[0046] (3) adding the mixture into an extraction device, performing microwave and ultrasonic extraction alternately under nitrogen conditions, with a microwave power of 6 kW, an ultrasonic power of 60 kHz, and ultrasonic and microwave extraction times of 4 times, each time for 10 min, to obtain an extract;

[0047] (4) The extract is subjected to microfiltration with a micropore porosity of less than 5 μm, followed by centrifugation to obtain a clear oil, namely, a natural fruit seed oil composition.

[0048] like Figure 1 As shown in the middle figure, the obtained natural fruit seed oil composition is a uniform yellow-orange transparent oil, and the smell is a nutty bitter almond smell with a sweet taste.

[0049] Example 3

[0050] A method for preparing a natural fruit seed oil composition with high antioxidant activity comprises the following steps:

[0051] (1) Astragalus membranaceus and Glycyrrhiza glabra are mixed in a mass ratio of 1:1 and dried to a water content of about 10%, and then the temperature of the grinding equipment is controlled at about -80°C, and the Astragalus and Glycyrrhiza glabra are ground into Chinese medicine powder in a temperature range of -80°C to -50°C, and the particle size of the powder after grinding and sieving is less than 80 μm;

[0052] (2) 22wt% of Chinese medicine micropowder is impregnated and mixed with 78wt% of oil composition to obtain a mixture, wherein the oil composition includes 51wt% of jojoba (SIMMONDSIA CHINENSIS) seed oil and 27wt% of fruit seed oil, and the fruit seed oil includes 5wt% of raspberry (RUBUS IDAEUS) seed oil, 2wt% of vaccinium macrocarpon (VACCINIUMMACROCARPON) seed oil, 3wt% of grape (VITIS VINIFERA) seed oil, 2wt% of small-fruit coffee (COFFEAARABICA) seed oil, 5wt% of dog rose (ROSACANINA) fruit oil, 5wt% of pomegranate (PUNICAGRANATUM) seed oil and 5wt% of European sour cherry (PRUNUS CERASUS) seed oil;

[0053] (3) adding the mixture into an extraction device, performing microwave and ultrasonic extraction alternately under nitrogen conditions, with a microwave power of 6 kW, an ultrasonic power of 70 kHz, and ultrasonic and microwave extraction times of 4 times, each time for 12 min, to obtain an extract;

[0054] (4) The extract is subjected to microfiltration with a micropore porosity of less than 5 μm, followed by centrifugation to obtain a clear oil, namely, a natural fruit seed oil composition.

[0055] like Figure 1 As shown in the middle right figure, the obtained natural fruit seed oil composition is a uniform orange transparent oil with a nutty bitter almond smell and a sweet taste.

[0056] Example 4

[0057] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (1), the mass ratio of Astragalus membranaceus to Glycyrrhiza glabra is 2:1.

[0058] Example 5

[0059] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (1), the mass ratio of Astragalus membranaceus to Glycyrrhiza glabra is 1:3.

[0060] Example 6

[0061] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (1), astragalus and licorice are ground into Chinese medicine powder at a temperature range of -80°C to -50°C, and the particle size of the powder after grinding and sieving is between 100-200 μm.

[0062] Example 7

[0063] The invention discloses a method for preparing a natural fruit seed oil composition with high antioxidant activity. The steps and the reagents, equipment and process parameters used in the steps are the same as those in Example 2, except that in step (2), the oil composition comprises 53 wt% of jojoba (SIMMONDSIA CHINENSIS) seed oil and 27 wt% of fruit seed oil, and the fruit seed oil comprises 4.2 wt% of raspberry (RUBUS IDAEUS) seed oil, 3.6 wt% of vaccinium macrocarpon (VACCINIUM MACROCARPON) seed oil, 2 wt% of grape (VITIS VINIFERA) seed oil, 2.6 wt% of coffee (COFFEAARABICA) seed oil, 5.8 wt% of dog rose (ROSA CANINA) fruit oil, 4.2 wt% of pomegranate (PUNICAGRANATUM) seed oil and 4.6 wt% of European sour cherry (PRUNUS CERASUS) seed oil.

[0064] Example 8

[0065] The invention discloses a method for preparing a natural fruit seed oil composition with high antioxidant activity. The steps and the reagents, equipment and process parameters used in the steps are the same as those in Example 2, except that in step (2), the oil composition comprises 53 wt% of jojoba (SIMMONDSIACHINENSIS) seed oil and 27 wt% of fruit seed oil, and the fruit seed oil comprises 2.5 wt% of raspberry (RUBUS IDAEUS) seed oil, 5.5 wt% of vaccinium macrocarpon (VACCINIUM MACROCARPON) seed oil, 4 wt% of grape (VITIS VINIFERA) seed oil, 5.5 wt% of coffee (COFFEAARABICA) seed oil, 2.5 wt% of dog rose (ROSACANINA) fruit oil, 5 wt% of pomegranate (PUNICA GRANATUM) seed oil and 2 wt% of European sour cherry (PRUNUS CERASUS) seed oil.

[0066] Example 9

[0067] The invention discloses a method for preparing a natural fruit seed oil composition with high antioxidant activity. The steps and the reagents, equipment and process parameters used in the steps are the same as those in Example 2, except that in step (2), the oil composition comprises 53 wt% of jojoba (SIMMONDSIA CHINENSIS) seed oil and 27 wt% of fruit seed oil, and the fruit seed oil comprises 3.5 wt% of raspberry (RUBUS IDAEUS) seed oil, 4 wt% of vaccinium macrocarpon (VACCINIUM MACROCARPON) seed oil, 5 wt% of grape (VITIS VINIFERA) seed oil, 5 wt% of coffee (COFFEA ARABICA) seed oil, 3.5 wt% of dog rose (ROSACANINA) fruit oil, 3 wt% of pomegranate (PUNICAGRANATUM) seed oil and 3 wt% of European sour cherry (PRUNUS CERASUS) seed oil.

[0068] Example 10

[0069] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (2), the oil composition comprises jojoba (SIMMONDSIA CHINENSIS) seed oil accounting for 43wt% of the system and fruit seed oil accounting for 37wt%, and the ratio of raspberry (RUBUS IDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, coffee (COFFEAARABICA) seed oil, dog rose (ROSACANINA) fruit oil, pomegranate (PUNICAGRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in the fruit seed oil is the same as that in Example 2, and the specific ratio is 5.2:2.5:2.5:1.2:5.2:5.2:5.2.

[0070] Embodiment 11

[0071] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (2), the oil composition comprises 60 wt% of jojoba (SIMMONDSIACHINENSIS) seed oil and 20 wt% of fruit seed oil, and the ratio of raspberry (RUBUS IDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, coffee (COFFEA ARABICA) seed oil, dog rose (ROSA CANINA) fruit oil, pomegranate (PUNICAGRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in the fruit seed oil is the same as that in Example 2, and the specific ratio is 5.2:2.5:2.5:1.2:5.2:5.2:5.2.

[0072] Comparative Example 1

[0073] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (2), 20wt% of Chinese medicine micropowder is impregnated and mixed with 80wt% of the oil composition to obtain a mixture, and the oil composition is jojoba (SIMMONDSIA CHINENSIS) seed oil.

[0074] Comparative Example 2

[0075] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (1), Astragalus membranaceus is dried at low temperature to a water content of about 5%, and then the temperature of the grinding equipment is controlled at about -80°C, and the Astragalus is ground into Chinese medicine powder within a temperature range of -80°C to -50°C, and the particle size of the powder after grinding is <100 μm.

[0076] Comparative Example 3

[0077] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (1), Glycyrrhiza glabra (GLYCYRRHIZA GLABRA) is dried at low temperature to a water content of about 5%, and then the temperature of the grinding equipment is controlled at about -80°C, and the Glycyrrhiza glabra is ground into Chinese medicine powder within a temperature range of -80°C to -50°C, and the particle size of the powder after grinding is less than 100 μm.

[0078] Comparative Example 4

[0079] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (2), the oil composition comprises 70 wt% of jojoba (SIMMONDSIACHINENSIS) seed oil and 10 wt% of fruit seed oil, and the ratio of raspberry (RUBUS IDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, coffee (COFFEAARABICA) seed oil, dog rose (ROSACANINA) fruit oil, pomegranate (PUNICAGRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in the fruit seed oil is the same as that in Example 2, and the specific ratio is 5.2:2.5:2.5:1.2:5.2:5.2:5.2.

[0080] Comparative Example 5

[0081] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (2), the oil composition comprises 53 wt% of jojoba (SIMMONDSIACHINENSIS) seed oil and 27 wt% of fruit seed oil, and the ratio of vaccinium macrocarpon (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in the fruit seed oil is the same as that in Example 2, specifically, the ratio is 2.5:2.5:5.2.

[0082] Comparative Example 6

[0083] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (2), the oil composition comprises 53 wt% of jojoba (SIMMONDSIACHINENSIS) seed oil and 27 wt% of fruit seed oil, and the ratio of raspberry (RUBUS IDAEUS) seed oil, small-fruit coffee (COFFEA ARABICA) seed oil, pomegranate (PUNICAGRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in the fruit seed oil is the same as that in Example 2, and the specific ratio is 5.2:1.2:5.2:5.2.

[0084] Comparative Example 7

[0085] A method for preparing a natural fruit seed oil composition with high antioxidant activity, wherein each step and the reagents, equipment and process parameters used in each step are the same as those in Example 2, except that in step (2), the oil composition comprises 53 wt% of jojoba (SIMMONDSIACHINENSIS) seed oil and 27 wt% of fruit seed oil, and the ratio of vaccinium macrocarpon (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, rosa canina (ROSACANINA) fruit oil and pomegranate (PUNICA GRANATUM) seed oil in the fruit seed oil is the same as that in Example 2, and the specific ratio is 2.5:2.5:5.2:5.2.

[0086] Application Example 1

[0087] A cosmetic with high antioxidant activity comprises 28.8 wt % of isohexadecane, 10 wt % of squalane, 20 wt % of isononyl isononanoate, 20 wt % of macadamia seed oil, 20 wt % of caprylic capric triglyceride, 0.5 wt % of vitamin E, 0.5 wt % of essence, and 0.2 wt % of the natural fruit seed oil composition of Example 2.

[0088] Comparative application example 1

[0089] A cosmetic comprises 28.8 wt % of isohexadecane, 10 wt % of squalane, 20 wt % of isononyl isononanoate, 20 wt % of macadamia seed oil, 20 wt % of caprylic capric triglyceride, 0.5 wt % of vitamin E, 0.5 wt % of essence and 0.2 wt % of jojoba seed oil.

[0090] Performance testing

[0091] 1. In vitro antioxidant activity assay: The in vitro antioxidant activity of the composition of the embodiment or comparative example was assayed according to the DPPH and ABTS methods in the polypeptide oxidative activity assay of GBT39100-2020.

[0092] DPPH is a dark purple prismatic crystal that is dark purple in anhydrous ethanol solution. The DPPH free radical has a strong absorption peak at 517nm in the visible light region. The method combines a stable DPPH free radical provided by DPPH with an electron provided by an antioxidant active substance to make the characteristic purple color of the DPPH free radical disappear and turn it into colorless or light yellow. The absorbance after the reaction is measured by an ultraviolet-visible spectrophotometer to detect the free radical scavenging rate of the 3.125wt% concentration natural fruit seed oil composition on a 50ppm DPPH solution. The measurement results are shown in Table 2 below.

[0093] 2. Determination of Glabridin Content: The glabridin content of the composition prepared in the embodiment or comparative example was determined by high performance liquid chromatography. The determination results are shown in Table 2 below, which comprises the following steps:

[0094] 1) Accurately weigh 0.5g oil sample (accurate to 0.001g) into a 10ml volumetric flask, add anhydrous ethanol and ultrasonically extract for 10min, then make up to volume, filter with a 0.22um organic phase filter membrane into a 2ml liquid phase injection vial for later use;

[0095] 2) Prepare the standard curve of 2 mg / L, 5 mg / L, 10 mg / L, 15 mg / L and 20 mg / L of glabridin standard using anhydrous ethanol, transfer into 2 ml liquid phase injection vials for later use;

[0096] 3) Agilent 1220 InfinityⅡLC high performance liquid chromatograph was used for injection analysis. The specific conditions were as follows: chromatographic column: Agilent 5TC-C18(2) 250*4.6 mm; column temperature: 30 degrees; detection wavelength: 282 nm; injection volume: 5 uL; mobile phase: 45% perchloric acid aqueous solution (0.08%): 55% acetonitrile; flow rate: 1 ml / min.

[0097] 3. Determination of total polyphenols content, including the following steps:

[0098] 1) Accurately weigh 3 g of the oil sample (accurate to 0.001 g) in a 15 ml screw-cap plastic centrifuge tube, add 3 ml of methanol, vortex and mix, perform ultrasonic extraction for 10 min, centrifuge at 10000 rpm for 10 min, take the supernatant methanol layer in another 15 ml screw-cap plastic centrifuge tube, add 3 ml of methanol to the remaining oil phase, repeat the extraction centrifugation operation once, combine the methanol layers, add 3 ml of n-heptane to about 6 ml of the extracted methanol layer, vortex and mix, centrifuge at 10000 rpm for 10 min, discard the n-heptane layer, and keep the methanol layer as the sample solution;

[0099] 2) Oleuropein (CAS32619-42-4) was used as a standard and diluted stepwise with methanol to obtain 100 mg / L, 200 mg / L, 500 mg / L, 800 mg / L, and 1000 mg / L standard solutions. The pretreatment process of the standard curve was the same as that of the sample;

[0100] 3) Add 5 ml of deionized water, 2 ml of the prepared sample solution, and 1 ml of Folin phenol reagent to a 15 ml centrifuge tube, shake well and let stand for 3 min, add 5 ml of 10% Na2CO3 solution, shake well, and let stand in the dark for 30 min;

[0101] 4) The absorbance of the color developing solution was measured at 760 nm using a spectrophotometer to calculate the polyphenol content in the sample. The test results are shown in Table 2 below.

[0102] 4. Determination of peroxide value, including the following steps:

[0103] 1) Weigh 5g of oil sample (accurate to 0.001g) into the titration cup of the potentiometric titrator, add 50mL of isooctane-glacial acetic acid mixture, shake gently to dissolve the sample completely, accurately add 0.5mL of saturated potassium iodide solution into the titration cup, start the magnetic stirrer, react for 60s±1s at a suitable stirring speed, immediately add 100mL of water into the titration cup, insert the electrode and titration head, set the titration parameters, run the titration program, use the dynamic titration mode to titrate and observe the titration curve and potential changes. The amount of sodium thiosulfate standard solution added is generally controlled at 0.1mL / drop. After reaching the titration endpoint, record the volume V of the standard solution consumed at the titration endpoint;

[0104] 2) At the same time, a blank test is performed, and the titration is performed in equal titration mode and the titration curve and potential changes are observed. The amount of sodium thiosulfate standard solution added is generally controlled at 0.005 mL / drop. After reaching the titration endpoint, the volume of standard solution consumed at the titration endpoint is recorded. The volume of 0.01 mol / L sodium thiosulfate solution consumed in the blank test shall not exceed 0.1 mL.

[0105] 3) The peroxide value was calculated according to the formula. The test results are shown in Table 2 below.

[0106] The peroxide value P is calculated according to the following formula:

[0107]

[0108] Wherein, V is the volume of sodium thiosulfate solution used for determination, in mL; V0 is the volume of sodium thiosulfate solution used for blank test, in mL; c is the concentration of sodium thiosulfate solution, in mol / L; m is the mass of the sample, in g.

[0109] 5. Antioxidant stability: Place the sample in an electric constant temperature incubator at 40°C, seal it and place it for 15 days. Measure the peroxide value. The test results are shown in Table 2 below.

[0110] 6. Human efficacy testing, including the following steps:

[0111] 1) Test environment temperature: 20℃-22℃, humidity: 40%-60%, and real-time dynamic monitoring;

[0112] Number of subjects: 8 effective subjects, divided into experimental group (application example 1) and control group (comparative application example 1), with 4 effective samples in each group;

[0113] Inclusion criteria: subjects aged 18-45 years, with dark yellow, dull and pigmented skin, in good general health and without major systemic diseases;

[0114] Exclusion criteria: women who are currently pregnant, breastfeeding or preparing for pregnancy; those with facial damage, inflammation or other skin diseases; those with highly sensitive constitutions or allergies to cosmetics.

[0115] 2) Preparation before the test: The test area is the face. No products can be used on the test area on the day of the test. Before the test, the subject cleans the test area, calibrates the measurement area, and sits quietly in the human efficacy evaluation room (temperature: 20℃-22℃, humidity: 50%-60%) for 30 minutes, avoiding touching and not eating during this period.

[0116] 3) How to use the test product: After cleansing your face in the morning and evening, apply 2 pumps of the sample evenly on your face and gently massage until completely absorbed. Use for 28 consecutive days. Do not use other products with whitening effects during this period.

[0117] 4) Test plan: 30 minutes after cleaning, use Antera 3D multifunctional 3D skin imaging analyzer to take pictures and analyze, and measure the ITA value in the test area (AOI, Area of ​​Interest), and use Delfin VapoMeter transepidermal water loss meter and SkinGlossMeter skin brightness meter to measure and analyze in all test areas as the initial value (D0), then distribute the product and guide the use method. After the subjects have used the product continuously for 7 days, 14 days, and 28 days, they will return for a visit and measure the same indicators. Each measurement will be carried out in parallel for each area for at least 3 times, and the difference between the maximum and minimum values ​​of the recorded data shall not exceed 1 unit, and the average value of the values ​​shall be calculated. The test of the same subject shall be completed by the same tester using the same instrument, and the test site shall be kept consistent between the two test time points. The test results are shown in Table 3 below.

[0118] Table 2 - Performance test results of the compositions of the present application examples or comparative examples

[0119]

[0120]

[0121] As shown in Table 2, Examples 1-3 are as follows: raspberry (RUBUS IDAEUS) seed oil, vaccinium macrocarpon (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, coffee (COFFEAARABICA) seed oil, dog rose (ROSA CANINA) fruit oil, pomegranate (PUNICA GRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil, the oil molecules of natural plant oils contain polar functional groups, and some natural plant oils also carry weak polar substances. By combining these polar functional groups with the antioxidant molecules in the extracts of Astragalus and Licorice, the content of antioxidant active ingredients in the composition can be increased, and the polyphenols, tocopherols and other natural ingredients contained in the natural plant oils are conducive to improving the antioxidant and stability of oils and antioxidant active molecules, avoiding the failure of antioxidant active molecules, and the content of glabridin and calycosin isoflavones and total polyphenols in the composition are significantly increased, and the peroxide value is significantly reduced; and the antioxidant active molecules form a micro-micelle stabilization system in the fruit seed oil system, and the antioxidant activity is still guaranteed to be high when stored in a high temperature environment for a long time. However, in Comparative Example 1, no natural fruit seed oil is added, and only jojoba oil is used to extract the Chinese medicine micropowder at low temperature. The prepared composition has a low polyphenol content, the content of active antioxidant components in the Chinese medicine is reduced, the antioxidant performance is reduced, and the high temperature storage stability is poor.

[0122] As shown in Table 2, in Comparative Example 2, only the Chinese medicinal ingredient of Astragalus was added, and the antioxidant active ingredient of Glycyrrhiza glabra was lacking, and the antioxidant capacity was obviously inferior to that of Example 2; in Comparative Example 3, only the Chinese medicinal ingredient of Glycyrrhiza glabra was added, and the polyphenols, antioxidant and other active ingredients of Astragalus were lacking, so that the antioxidant activity of the final composition was significantly reduced, the total polyphenol content was less, and the antioxidant stability stored in a long-term high temperature environment was poor.

[0123] Table 3 - Human efficacy test results of cosmetics in the application examples of this application and the comparative application examples

[0124]

[0125]

[0126] As shown in Table 3, compared with the initial value, the skin color ITA value and skin brightness L value of Application Example 1 have different degrees of improvement at D28, indicating that the antioxidant effect is good and promotes skin whitening, and the transepidermal water loss rate TEWL decreases at D28, and the water loss is slow. The human test results prove that the antioxidant and moisturizing effects are good. In contrast, the skin color ITA value and skin brightness L value of Application Example 1 are equivalent to the initial value D0 at D28, especially the skin brightness L value, indicating that the antioxidant capacity is insufficient, and the transepidermal water loss rate TEWL is not much different at D28, indicating that the moisturizing property has not been significantly improved.

[0127] The specific embodiments described above further illustrate the purpose, technical solutions and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. It is particularly pointed out that for those skilled in the art, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of protection of the present invention.

Claims

1. A natural fruit seed oil composition with high antioxidant activity, characterized in that: The natural fruit seed oil composition is obtained by extracting, filtering and centrifuging 15wt%-30wt% of Chinese medicine micropowder and the rest of the oil composition; the oil composition comprises 30wt%-60wt% of jojoba (SIMMONDSIA CHINENSIS) seed oil and 20wt%-65wt% of fruit seed oil, which account for 30wt%-60wt% of the mass fraction of the natural fruit seed oil composition; The fruit seed oil comprises raspberry (RUBUS IDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, small-fruit coffee (COFFEA ARABICA) seed oil, dog rose (ROSA CANINA) fruit oil, pomegranate (PUNICA GRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in a mass ratio of (2.5-5.5): (2-5.5): (2-5): (1-5.5): (2.5-5.8): (3-5.5): (3-5.5).

2. A natural fruit seed oil composition with high antioxidant activity as claimed in claim 1, characterized in that: The Chinese medicine micro powder comprises astragalus and liquorice in a mass ratio of (1-2):(1-3).

3. A natural fruit seed oil composition with high antioxidant activity as claimed in claim 1, characterized in that: The fruit seed oil includes raspberry (RUBUSIDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, small-fruit coffee (COFFEA ARABICA) seed oil, dog rose (ROSA CANINA) fruit oil, pomegranate (PUNICA GRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in a mass ratio of (5-5.5): (2-3): (2-3): (1-2): (5-5.5): (5-5.5): (5-5.5).

4. A natural fruit seed oil composition with high antioxidant activity as claimed in claim 1, characterized in that: The fruit seed oil includes raspberry (RUBUS IDAEUS) seed oil, blueberry (VACCINIUM MACROCARPON) seed oil, grape (VITIS VINIFERA) seed oil, small-fruit coffee (COFFEA ARABICA) seed oil, dog rose (ROSA CANINA) fruit oil, pomegranate (PUNICAGRANATUM) seed oil and European sour cherry (PRUNUS CERASUS) seed oil in a mass ratio of 5.2:2.5:2.5:1.2:5.2:5.2:5.

2.

5. A natural fruit seed oil composition with high antioxidant activity as claimed in claim 1, characterized in that: The oil composition comprises 43wt%-54wt% of jojoba (SIMMONDSIA CHINENSIS) seed oil and 27wt%-37wt% of fruit seed oil.

6. A method for preparing a natural fruit seed oil composition with high antioxidant activity as claimed in any one of claims 1 to 5, characterized in that: The following steps are involved: (1) drying the astragalus and licorice to a moisture content of less than 20%, and then grinding the astragalus and licorice into Chinese medicine fine powder at a temperature ranging from -80°C to -50°C; (2) impregnating and mixing the Chinese medicine powder with the oil composition to obtain a mixture; (3) adding the mixture into an extraction device, and performing microwave and ultrasonic extraction alternately under nitrogen conditions to obtain an extract; (4) The extract is subjected to microfiltration and then centrifugation to obtain a natural fruit seed oil composition.

7. The method for preparing a natural fruit seed oil composition with high antioxidant activity as claimed in claim 6, characterized in that: In step (1), the particle size of the Chinese medicine micropowder after grinding is less than 100 μm.

8. The method for preparing a natural fruit seed oil composition with high antioxidant activity as claimed in claim 6, characterized in that: In step (3), the microwave power is 4kW-6kW, the ultrasonic power is 10kHz-80kHz, the ultrasonic and microwave extraction times are both 4-6 times, and the duration of each time is 1min-15min.

9. Use of the natural fruit seed oil composition with high antioxidant activity as claimed in any one of claims 1 to 8 in the preparation of cosmetics.

10. A cosmetic with high antioxidant activity, characterized in that: A natural fruit seed oil composition with high antioxidant activity as described in any one of claims 1 to 8 is used, comprising the following components in parts by weight: 25 to 30 parts of isohexadecane, 8 to 12 parts of squalane, 18 to 22 parts of isononyl isononanoate, 18 to 22 parts of macadamia seed oil, 18 to 22 parts of caprylic capric triglyceride, 0.3 to 1 part of vitamin E, 0.1 to 0.5 parts of flavor, and 0.1 to 5 parts of natural fruit seed oil composition.

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