Cosmetic composition containing gastrodia elata extract as well as preparation method and application of cosmetic composition
By adding ingredients such as Gastrodia elata extract to the cosmetic composition, it is solved that existing cosmetics are difficult to effectively solve skin aging, wrinkles, moisturizing and other problems, and achieve significant repair, anti-wrinkle, firming, soothing and moisturizing effects, and is suitable for sensitive skin.
Patent Information
- Application Number
- CN202311112138.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-08-31
- Publication Date
- 2025-02-28
AI Technical Summary
Existing cosmetic compositions are difficult to effectively solve problems such as skin aging, wrinkles, loose skin, insignificant moisturizing effect and sensitive skin.
A cosmetic composition containing Gastrodia elata extract is adopted, which contains glycerin, beetroot extract, Gastrodia elata extract and other ingredients. It is used in the form of a facial mask to provide repair, anti-wrinkle, firming, soothing and moisturizing effects.
The cosmetic composition and facial mask have significant repair, anti-wrinkle, firming, soothing and moisturizing effects. It is suitable for sensitive skin, with low toxicity, low irritation and high safety.
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Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of skin care products, and in particular to a cosmetic composition comprising a gastrodia elata extract, and a preparation method and application thereof. Background Art
[0002] As we age, our facial skin ages, and wrinkles and sagging follow. The aging of facial skin is mainly caused by insufficient blood supply and the deposition of toxins, which has a serious impact on people's image. The skin is located on the surface of the human body and is the first line of defense for the human body. In particular, the stratum corneum of the skin has a very important function. The water in the stratum corneum is an important plastic, and its relative content is about 10% to 15%. As we age, the content of natural moisturizing factors in the stratum corneum of the skin gradually decreases, causing the skin's hydration capacity to decrease to only 75% of normal skin. At the same time, the skin is affected by endogenous factors, and will show sagging, fine wrinkles, dryness, desquamation, increased brittleness, and decreased repair function.
[0003] It is known that skin wrinkles are affected by various factors, such as skin moisture content, collagen content and immunity to the external environment, collagen production and collagenase expression and activity. Among them, the gradual decrease in skin water content is one of the pathological mechanisms of aging appearance. When the water content of the stratum corneum is less than 10%, the skin becomes dry and flaky, and cracks appear in the stratum corneum layer arranged in parallel. The skin is easily eroded by physical factors of the external environment, and the water and electrolytes in the skin are also easily evaporated or lost. Skin moisturizing is the most important basic means to ensure the barrier function of the stratum corneum and skin care beauty. Moisturizing products mainly prevent the loss of water in the skin and absorb moisture from the external environment to achieve the purpose of containing a certain amount of water in the skin. At present, the more recognized moisturizing active ingredients are bionic agents, such as ceramide, hyaluronic acid, sodium pyrrolidonecarboxylate, etc. After being absorbed by the skin, they can combine with free water in the skin to make it less likely to volatilize and thus play a moisturizing role. The main problem is that the chemical properties of such ingredients are unstable, and the content in the product system is often gradually reduced due to internal and external factors, and it cannot reach the effective threshold, and its own moisturizing effect is not significant.
[0004] Sensitive skin refers to skin that is easily stimulated and causes some degree of discomfort. Inflammatory factors are the key factors in the formation of sensitive skin. The skin inflammation caused by them is reflected in three aspects: on the one hand, the human skin barrier function decreases, resulting in an increase in transepidermal water loss and irritant penetration; on the other hand, nerve sensitivity increases, resulting in an increase in nerve growth factor and enhanced stimulation of inflammatory mediators; finally, blood vessel dilation and increased permeability lead to facial flushing. The inflammatory reaction ultimately leads to: 1. The skin epidermis is thin, the cortex secretes less, it is drier, the microvessels are obvious, the skin shows a dry function decline, the ability of the stratum corneum to retain moisture is reduced, and the sebum film on the surface of the skin is not fully formed. 2. The skin is prone to unstable state due to seasonal changes. The main symptoms are itching, burning, stinging, itchy skin and rash. 3. It is easy to cause skin allergies after contact with cosmetics or seasonal allergies, resulting in redness, swelling and itching. The skin lacks luster, and the cheeks are prone to congestion and swelling. 4. It is easily affected by external environment or substances such as cold wind, ultraviolet rays, synthetic fibers, fragrance, pigments, food, water quality, etc. 5. When exposed to irritating substances such as plant pollen, cosmetics, perfume, etc., skin problems will occur. However, existing cosmetic compositions or facial masks and other products do not effectively solve the above problems. In view of this, the present invention is proposed. Summary of the invention
[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art, provide a cosmetic composition comprising a Gastrodia elata extract, a preparation method and application thereof, and provide a facial mask comprising the cosmetic composition. The cosmetic composition and facial mask have the advantages of repairing, anti-wrinkle, firming, soothing, moisturizing, low irritation, high safety, suitable for sensitive skin, and high acceptance.
[0006] In order to solve the above technical problems, the first technical solution adopted by the present invention is: a cosmetic composition, in which the following components are included in the cosmetic composition by mass percentage: 1-3% glycerin, 1-2% dipropylene glycol, 0.8-1.2% beetroot extract, 0.3-0.6% preservative-free, 0.3-0.6% polydimethylsiloxane, 0.1-0.3% allantoin, 0.1-0.3% 1,2-hexanediol, 0.1-0.2% cell revitalizing biopeptide, and 0.1-0.2% polyglycerol-10 Oleate 0.1-0.2%, Saccharomyces black tea fermentation product 0.1-0.2%, p-hydroxyacetophenone 0.1-0.2%, jojoba seed oil 0.1-0.13%, carbomer 0.1-0.13%, Gastrodia elata extract 0.1-0.13%, dipotassium glycyrrhizinate 0.8-0.11%, hydrolyzed sclerotium gum 0.9-0.11%, purple snow ginseng oil 0.9-0.11%.
[0007] More preferably, the cosmetic composition further comprises the following components, measured in percentage by mass: 0.06-0.07% tromethamine, 0.03-0.05% hydroxyethyl cellulose, 0.006-0.01% rosewood oil, 0.0006-0.0015% giant kelp extract, 0.0006-0.0015% ambergris fruit extract, 0.0006-0.0015% jasmine leaf cell extract, 0.0006-0.0001% light peptide, 0.0006-0.0015% cordyceps extract, 0.0006-0.0015% hyaluronic acid, 0.0006-0.0015% sea fennel callus culture filtrate, and 0.0006-0.0015% Centella asiatica extract.
[0008] Optimally, the cosmetic composition comprises the following components by mass percentage: 92.7552% water; 2% glycerin; 1.5% dipropylene glycol; 1% beetroot extract; 0.5% preservative-free; 0.5% polydimethylsiloxane; 0.2% allantoin; 0.2% 1,2-hexanediol; 0.15% cell revitalizing biopeptide; 0.15% polyglycerol-10 Oleate 0.15%, Saccharomyces black tea fermentation product 0.15%, p-hydroxyacetophenone 0.15%, jojoba seed oil 0.11%, carbomer 0.11%, CMZZ-Gastrodia extract 0.11%, dipotassium glycyrrhizinate 0.101%, hydrolyzed sclerotium gum 0.1%, purple snow ginseng oil 0.1%, tromethamine 0.065%, hydroxyethyl cellulose 0.04%, rosewood oil 0.008%, giant kelp extract 0.0001%, coffee sunflower fruit extract 0.0001%, jasmine leaf cell extract 0.0001%, time peptide 0.0001%, cordyceps extract 0.0001%, hyaluronic acid 0.0001%, sea fennel callus culture filtrate 0.0001%, Centella asiatica extract 0.0001%.
[0009] The components are conventional raw materials in the art, preferably: 1. Water, 2. Glycerin 99.5% USP, 3. Dipropylene glycol (Dipropylene Glycol) DPG, 4. Beetroot extract trade name: Meihujian ® BE, 5. No added preservatives HL-B91, 6. Polydimethylsiloxane KF-96-350CS, 7. Allantoin; 8. 1,2-hexanediol EFFISIN HD, 9. Cell revitalizing biopeptide trade name: EMGPEPTIDE ® –BN Pro, 10. Polyaldo™ 10-1-O KFG, 11. Saccharomyces cerevisiae black tea fermentation product, 12. Parahydroxyacetophenone Trade name: SymSave ®H, 13. Jojoba seed oil DW JOJOBA COLORLESS, 14. Carbomer trade name is: CARBOPOL ® 940 POLYMER, 15. CMZZ-Gastrodia elata extract, 16. Dipotassium glycyrrhizinate, 17. Hydrolyzed sclerotium gum Chcogum HG, 18. Ganoderm T, 19. Cosmecare 715 tromethamine, 20. The trade name of hydroxyethyl cellulose is: Natrosol™ 250HHR PC HY, 21. ROSE WOOD OIL, 22. The trade name of giant kelp extract is: Meihujian ® CDMC_MZ, 23. The trade name of coffee sunflower fruit extract is: Meihujian ® AE 100, 24. LightWaves Defense [JS+M], 25. Time peptide, 26. Cordyceps sinensis extract, 27. EPIDERMOSIL SP, 28. CELTOSOME CRITHMUM MARITIMUM ST, 29. CENTELLA ASIATICA EXTRACT (WS) CH. The above components can be prepared and synthesized by conventional methods in the art, or obtained by commercial purchase.
[0010] The term "plant extract" or "extract" generally refers to an isolated substance obtained by extraction from a plant material, which itself does not pre-exist in nature.
[0011] The plant extract or extracts of the present invention are implemented in the composition in the form of a solution or a concentrate according to the present invention, or are referred to as "products containing the extracts", and the solvent of the concentrate may be an extraction solvent and / or an additional solvent. Therefore, the extracts shown in the composition examples are in the form of solutions. Reference will also be made to the products or products each containing the extract, some of which are commercial products.
[0012] Those skilled in the art know that plants or plants in general can produce many substances of cosmetic interest. Depending on the type of plant or flower used, these substances of interest can be present in different parts or organs of the plant, in particular in seeds, roots, stems, leaves, petals, flowers, corollas, fruits. For the same plant, or for the same flower, different extracts, in particular extracts of seeds, roots, bulbs, stems, leaves, petals, flowers, flowering tops, fruits will have different compositions of active molecules and therefore will have complementary cosmetic properties or even synergistic effects.
[0013] In the context of the present invention, the combination is characterized in that each plant extract is obtained from a part of the plant selected from the group consisting of seeds, roots, bulbs, stems, leaves, petals, flowers, corollas, fruits, the whole plant and mixtures thereof.
[0014] According to a specific embodiment, each plant extract is obtained from a part of the plant selected from fruits, roots and mixtures thereof. Many methods for extracting plant raw materials from the prior art are known, and those skilled in the art can easily implement and, if necessary, prepare the plant extract of the present invention using known methods.
[0015] In order to solve the above technical problems, the second technical solution adopted by the present invention is: a transmuscular facial mask containing Gastrodia elata extract, which comprises the cosmetic composition as described above, acceptable auxiliary materials in skin care products and acceptable solvents in skin care products.
[0016] Preferably, the skin-penetrating facial mask containing Gastrodia elata extract of the present invention comprises the following components, measured by mass percentage: 1-3% glycerin, 1-2% dipropylene glycol, 0.8-1.2% beetroot extract, 0.3-0.6% preservative-free, 0.3-0.6% polydimethylsiloxane, 0.1-0.3% allantoin, 0.1-0.3% 1,2-hexanediol, 0.1-0.3% cell revitalizing biopeptide, 0.1-0.2% polyglycerol-10 oleate, 0.1-0.2% yeast black tea fermentation product, 0.1-0.2% parahydroxyacetophenone, 0.1-0.2% jojoba seed oil, 0.1-0.13% carbomer, 0.1-0.13% Gastrodia elata extract, 0.8-0.11% dipotassium glycyrrhizate, 0.9-0.11% hydrolyzed sclerotium gum, and 0.9-0.11% purple snow ginseng nourishing oil.
[0017] More preferably, the skin-penetrating facial mask containing Gastrodia elata extract of the present invention further comprises the following components, measured in percentage by mass: 0.06-0.07% tromethamine, 0.03-0.05% hydroxyethyl cellulose, 0.006-0.01% rosewood oil, 0.0006-0.0015% giant kelp extract, 0.0006-0.0015% sunflower seed extract, 0.0006-0.0015% jasmine leaf cell extract, 0.0006-0.0015% light peptide, 0.0006-0.0015% cordyceps extract, 0.0006-0.0015% hyaluronic acid, 0.0006-0.0015% sea fennel callus culture filtrate, and 0.0006-0.0015% Centella asiatica extract.
[0018] Preferably, the transdermal facial mask containing Gastrodia elata extract of the present invention comprises the following components by weight percentage: 92.7552% water; 2% glycerin; 1.5% dipropylene glycol; 1% beetroot extract; 0.5% preservative-free agent; 0.5% polydimethylsiloxane; 0.2% allantoin; 0.2% 1,2-hexanediol; 0.15% cell revitalizing biopeptide; 0.15% polyglycerol-10 Oleate 0.15%, Saccharomyces black tea fermentation product 0.15%, p-hydroxyacetophenone 0.15%, jojoba seed oil 0.11%, carbomer 0.11%, CMZZ-Gastrodia extract 0.11%, dipotassium glycyrrhizinate 0.101%, hydrolyzed sclerotium gum 0.1%, purple snow ginseng oil 0.1%, tromethamine 0.065%, hydroxyethyl cellulose 0.04%, rosewood oil 0.008%, giant kelp extract 0.0001%, coffee sunflower fruit extract 0.0001%, jasmine leaf cell extract 0.0001%, time peptide 0.0001%, cordyceps extract 0.0001%, hyaluronic acid 0.0001%, sea fennel callus culture filtrate 0.0001%, Centella asiatica extract 0.0001%.
[0019] The components are conventional raw materials in the art, preferably: 1. Water, 2. Glycerin 99.5% USP, 3. Dipropylene glycol (Dipropylene Glycol) DPG, 4. Beetroot extract trade name: Meihujian ® BE, 5. No added preservatives HL-B91, 6. Polydimethylsiloxane KF-96-350CS, 7. Allantoin; 8. 1,2-hexanediol EFFISIN HD, 9. Cell revitalizing biopeptide trade name: EMGPEPTIDE ® –BN Pro, 10. Polyaldo™ 10-1-O KFG, 11. Saccharomyces cerevisiae black tea fermentation product, 12. Parahydroxyacetophenone Trade name: SymSave ® H, 13. Jojoba seed oil DW JOJOBA COLORLESS, 14. Carbomer trade name is: CARBOPOL ® 940 POLYMER, 15. CMZZ-Gastrodia elata extract, 16. Dipotassium glycyrrhizinate, 17. Hydrolyzed sclerotium gum Chcogum HG, 18. Ganoderm T, 19. Cosmecare 715 tromethamine, 20. The trade name of hydroxyethyl cellulose is: Natrosol™ 250HHR PC HY, 21. ROSE WOOD OIL, 22. The trade name of giant kelp extract is: Meihujian ®CDMC_MZ, 23. The trade name of coffee sunflower fruit extract is: Meihujian ® AE 100, 24. LightWaves Defense [JS+M], 25. Time peptide, 26. Cordyceps sinensis extract, 27. EPIDERMOSIL SP, 28. CELTOSOME CRITHMUM MARITIMUM ST, 29. CENTELLA ASIATICA EXTRACT (WS) CH. The above components can be prepared and synthesized by conventional methods in the art, or obtained by commercial purchase.
[0020] In order to solve the above technical problems, the third technical solution adopted by the present invention is: a method for preparing a cosmetic composition, the preparation method comprising the following steps: (1) Add the raw materials of phase A into the emulsifying pot, raise the temperature to 80-85℃ while stirring, stir for 8-10 minutes to mix evenly, and keep warm for 10 minutes. (2) Cool the product obtained in step (1) to 45-50°C, add the raw materials of phase B and phase C, and stir for 8-10 minutes; (3) Cooling the product obtained in step (2) to below 38°C, discharging the product, and allowing it to stand to obtain a finished product; The raw materials described in the preparation method and the mass percentage content of each raw material are: Phase A: Water 92.7552%; Glycerin 99.5% USP, 2%; Dipropylene glycol 1.5%; Dimethicone 0.5%; Allantoin 0.2%; Polyglyceryl-10 Oleate 0.15%; Parahydroxyacetophenone 0.15%, Jojoba Seed 0.11%, Carbomer 0.11%, Hydrolyzed Sclerotium Gum 0.1%, Hydroxyethylcellulose 0.04%; Phase B: Beetroot extract 1%, Cell Rejuvenating Biopeptide 0.15%, Saccharomyces cerevisiae black tea fermentation product 0.15%, Gastrodia elata extract 0.11%, Dipotassium glycyrrhizinate 0.101%, Purple Snow Ginseng Oil 0.1%, Sea Fennel Callus Culture Filtrate 0.0001%; Phase C: No added preservatives 0.5%, 1,2-hexanediol 0.2%, tromethamine 0.065%, rosewood oil 0.008%, giant kelp extract 0.0001%, ambrette fruit extract 0.0001%, jasmine leaf cell extract 0.0001%, time peptide 0.0001%, cordyceps extract 0.0001%, hyaluronic acid 0.0001%, Centella asiatica extract 0.0001%.
[0021] Preferably, in the preparation method of the present invention, the temperature in step (1) is 83°C and the stirring time is 9 minutes; the temperature in step (2) is 48°C and the stirring time is 10 minutes; the temperature in step (3) is 10-36°C and the standing time is 12-48 hours.
[0022] The raw materials are conventional raw materials in the art, preferably: 1. Water, 2. Glycerin 99.5% USP, 3. Dipropylene glycol (Dipropylene Glycol) DPG, 4. Beetroot extract trade name: Meihujian ® BE, 5. No added preservatives HL-B91, 6. Polydimethylsiloxane KF-96-350CS, 7. Allantoin; 8. 1,2-hexanediol EFFISIN HD, 9. Cell revitalizing biopeptide trade name: EMGPEPTIDE ® –BN Pro, 10. Polyaldo™ 10-1-O KFG, 11. Saccharomyces cerevisiae black tea fermentation product, 12. Parahydroxyacetophenone Trade name: SymSave ® H, 13. Jojoba seed oil DW JOJOBA COLORLESS, 14. Carbomer trade name is: CARBOPOL ® 940 POLYMER, 15. CMZZ-Gastrodia elata extract, 16. Dipotassium glycyrrhizinate, 17. Hydrolyzed sclerotium gum Chcogum HG, 18. Ganoderm T, 19. Cosmecare 715 tromethamine, 20. The trade name of hydroxyethyl cellulose is: Natrosol™ 250HHR PC HY, 21. ROSE WOOD OIL, 22. The trade name of giant kelp extract is: Meihujian ® CDMC_MZ, 23. The trade name of coffee sunflower fruit extract is: Meihujian ® AE 100, 24. LightWaves Defense [JS+M], 25. Time peptide, 26. Cordyceps sinensis extract, 27. EPIDERMOSIL SP, 28. CELTOSOME CRITHMUM MARITIMUM ST, 29. CENTELLA ASIATICA EXTRACT (WS) CH. The above components can be prepared and synthesized by conventional methods in the art, or obtained by commercial purchase.
[0023] After adopting the above technical scheme, the cosmetic composition containing Gastrodia elata extract and the preparation method thereof and a skin-penetrating facial mask containing the cosmetic composition of the present invention have the following beneficial effects compared with the prior art: 1. The cosmetic composition and the translucent facial mask containing the cosmetic composition have the functions and advantages of repairing, anti-wrinkle, firming, soothing and moisturizing the skin; 2. The cosmetic composition and the transdermal facial mask have low toxicity and irritation, can be applied to sensitive skin, and are highly accepted by the subjects; 3. The preparation method and process route of the cosmetic composition, the starting materials and reagents used are all commercial products, cheap and easily available, the technical scheme of the synthesis reaction is reasonable, the process flow has been simplified, and it is suitable for industrial production and meets large-scale commercial needs.
[0024] The specific embodiments of the present invention are described in further detail below. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] The accompanying drawings are part of the present invention and are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention, but do not constitute an improper limitation of the present invention. Obviously, the drawings described below are only some embodiments. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. In the accompanying drawings: Figure 1 This is a graph showing the results of a transepidermal water loss test of the cosmetic composition obtained in Example 1; Figure 2 This is a graph showing the free radical scavenging rate test results of the cosmetic composition obtained in Example 1; Figure 3 This is a graph showing the results of an elastase inhibition test of the cosmetic composition obtained in Example 1; Figure 4 This is a graph showing the results of a hyaluronidase inhibition test of the cosmetic composition obtained in Example 1; Figure 5 is the relative moisture retention change curve of the cosmetic composition obtained in Example 1; Figure 6 A comparison chart of the base value D0 and the test value D14 of the a* value of the cosmetic composition obtained in Example 1; Figure 7 This is a comparison chart of the EI value base value DO and the D14 test value of the cosmetic composition obtained in Example 1; Figure 8 This is a graph showing the evaluation results of the immediate satisfaction with the test product; Fig. 9 This is a chart showing the test subjects' evaluation of the product's effects.
[0026] It should be noted that these drawings and textual descriptions are not intended to limit the conceptual scope of the present invention in any way, but are intended to illustrate the concept of the present invention for those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION
[0027] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments will be described clearly and completely below. The following embodiments are used to illustrate the present invention, but are not used to limit the scope of the present invention. The chemical reagents used in the embodiments of the present invention are all conventional chemical reagents in the art. Unless otherwise specified, they are purchased from Sinopharm Chemical Reagent Co., Ltd.
[0028] Example 1 Preparation of a cosmetic composition containing Gastrodia elata extract For the percentage content, composition, INCI name, trade name and supplier of the above components, please see Table 1 and its continuation.
[0029] Table 1-1. Percentage content, composition, INCI name, trade name and supplier information of each component in the cosmetic composition
[0030] Table 1-2 (Continued Table 1)
[0031] Table 1-3 (Continued Table 1)
[0032] Table 1-4 (Continued Table 1)
[0033] Table 1-5 (Continued Table 1)
[0034] The preparation method of the above cosmetic composition is as follows: 1. Add the raw materials of phase A into the emulsifying pot, raise the temperature to 80-85℃ while stirring to mix evenly, and keep warm for 10 minutes. 2. Cool down to 45-50℃, add phase B and phase C materials, and stir thoroughly 3. Cool down to below 38℃, discharge and let stand. 4. After passing the inspection, it will be installed, packaged and inspected. 5. After passing the inspection, the goods will be put into storage.
[0035] The groups, serial numbers and mass percentages of the raw materials are as follows: Phase A: Water 92.7552%; Glycerin 99.5% USP, 2%; Dipropylene glycol 1.5%; Dimethicone 0.5%; Allantoin 0.2%; Polyglyceryl-10 Oleate 0.15%; Parahydroxyacetophenone 0.15%, Jojoba Seed 0.11%, Carbomer 0.11%, Hydrolyzed Sclerotium Gum 0.1%, Hydroxyethylcellulose 0.04%; Phase B: Beetroot extract 1%, Cell Rejuvenating Biopeptide 0.15%, Saccharomyces cerevisiae black tea fermentation product 0.15%, Gastrodia elata extract 0.11%, Dipotassium glycyrrhizinate 0.101%, Purple Snow Ginseng Oil 0.1%, Sea Fennel Callus Culture Filtrate 0.0001%; Phase C: No added preservatives 0.5%, 1,2-hexanediol 0.2%, tromethamine 0.065%, rosewood oil 0.008%, giant kelp extract 0.0001%, ambrette fruit extract 0.0001%, jasmine leaf cell extract 0.0001%, time peptide 0.0001%, cordyceps extract 0.0001%, hyaluronic acid 0.0001%, Centella asiatica extract 0.0001%.
[0036] According to the above preparation method, a cosmetic composition containing Gastrodia elata extract is prepared using the components described in Table 1.
[0037] Example 2 Toxicology test of cosmetic composition 1. Materials and Methods 1. Test substance: The cosmetic composition obtained in Example 1 was attached to a film cloth, and the liquid portion was taken for testing.
[0038] 2. Experimental animals and breeding environment: Experimental animals: 4 male common New Zealand rabbits, weighing 2.02-2.37 kg, with intact skin and no abnormalities. The experimental animals were purchased from Huadong Xinhua Experimental Animal Breeding Farm, Huadu District, Guangzhou City, with the experimental animal production license number: SCXK (Yue) 2019-0023 and the quality certificate number: No.44007600009918.
[0039] Feed: purchased from Beijing Huafukang Biotechnology Co., Ltd., production license: Beijing Feed Certificate (2019) 06076.
[0040] 3. Test method: Chapter 6 (5) of the Technical Specifications for Safety of Cosmetics (2015 edition) 3.1 Exposure method: Before the test, cut the hair on both sides of the spine of the experimental animal's back, the hair removal range is about 3cmx3cm on the left and right, and the application area is about 2.5cmx2.5cm. Apply 0.5mL of the test substance on one side of the skin, and the other side as a blank control, once a day, for 14 consecutive days. Starting from the second day, cut the hair before each application, and use water to remove the residual test substance. Observe the results after one hour. The control area and the test area are treated in the same way.
[0041] 3.2 Animal observation: The skin reaction was scored according to the skin irritation reaction scoring standard, and the skin irritation intensity was determined according to the skin irritation intensity grading standard using the following formula.
[0042]
[0043] 2. Test results Table 2. Results of multiple skin irritation tests on rabbits
[0044] 3. Test conclusion: The test substance caused multiple skin irritation to rabbits, and the results showed that the test substance was non-irritating to the skin.
[0045] Example 3 Acute eye irritation test of cosmetic composition 1. Materials and Methods 1. Test substance: The colorless liquid of the cosmetic composition obtained in Example 1 is attached to the membrane cloth, and the liquid portion is taken for testing.
[0046] 2. Experimental animals and breeding environment: Experimental animals: 3 male New Zealand rabbits of common grade, weighing 2.09-2.23 kg, with no abnormality in eyes after examination. The experimental animals were purchased from Xinhua Experimental Animal Farm, Huadong, Huadu District, Guangzhou, with the experimental animal production license number: SCXK (Yue) 2019-0023, and the quality certificate number: No.44007600009918.
[0047] Breeding environment: temperature 18-26°C, relative humidity 40-70%, experimental animal use license number: SYXK(Guangdong)2022-0198.
[0048] Feed: purchased from Beijing Huafukang Biotechnology Co., Ltd., production license: Beijing Feed Certificate (2019)06076.
[0049] 3. Test method: Chapter 6 (5) of the Technical Specifications for Safety of Cosmetics (2015 edition) 3.1 Exposure: Gently pull open the lower eyelid of one eye of the rabbit, and drop the test substance into the conjunctival sac at 0.1 mL / eye, so that the upper and lower eyelids are passively closed for 1 second. The other eye is not treated as a self-control. The eye is not rinsed within 24 hours after the test substance is dropped. After the 24-hour observation and recording, the eyes of all animals are further examined with sodium fluorescein.
[0050] 3.2 Animal Observation: The eyes of animals shall be examined at 1, 24, 48, 72 hours, and on the 4th and 7th days after the instillation of the test substance. If no irritation reaction occurs within 72 hours, the test can be terminated. If corneal involvement or other eye irritation is found, and the eye does not recover within 7 days, the observation time shall be extended to determine the reversibility or irreversibility of the damage, generally not exceeding 21 days, and observation reports for 7 days, 14 days and 21 days shall be provided. In addition to the observation of the cornea, iris and conjunctiva, other damaging effects shall be recorded and reported. In each examination, the score of eye irritation reaction shall be recorded according to the scoring standard for eye damage.
[0051] Table 3. Acute eye irritation test results
[0052] III. Test conclusion: According to the results in Table 3, the test substance is non-irritating to the acute eye irritation of rabbits without flushing.
[0053] Example 4 Experiment on removing freckles from cosmetic composition 1 Materials and Methods (1) The cosmetic composition prepared in Example 1; (2) Test method: The selected area shall not exceed 50mm 2 , depth of about 1mm qualified patch tester, add about 0.020g-0.025g (solid or semi-solid) or 0.020-0.025mL (liquid) of the test substance into the patch tester by closed patch test method, apply it to the curved side of the subject's forearm with low allergenic tape, press lightly with the palm of your hand to evenly apply it on the skin, remove the test substance after 24 hours, observe the skin reaction 0.5h (after the indentation disappears), 24h, and 48h after removal, and record the results according to the skin reaction grading standard in "Technical Specification for Safety of Cosmetics 2015".
[0054] Table 4 Grading criteria for adverse skin reactions Reaction severity rating scale Skin reaction - 0 Negative reaction ± 1 Suspected reaction, only slight erythema + 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, papules may be present ++ 3 Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes; reaction may extend beyond the test area +++ 4 Very strong positive reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, confluent herpes; reaction beyond the test area Table 5 Results of skin adverse reactions
[0055] Test Results: A total of 30 subjects were recruited, and 30 subjects completed the test. The results showed that the scores of all 30 subjects at the three observation time points of 0.5 h, 24 h, and 48 h after removing the patch were 0 points. Referring to the grading standard of skin adverse reactions in the "Technical Specifications for Cosmetics Safety" (2015 edition), 0 cases of skin adverse reactions occurred among the 30 subjects. The product did not cause skin adverse reactions, indicating relatively high product safety.
[0056] Example 5 Repair Test of Cosmetic Composition 1 Test Product: The cosmetic composition prepared in Example 1 2 Test Method: According to the experimental design, the enrolled subjects continuously used the test product for 2 weeks, once every two days. The trans-epidermal water loss rate of the subjects' skin was tracked and observed under the environmental conditions of temperature 21 ± 1°C and relative humidity 50 ± 10%. Photos and other data were collected at D0 and D14 respectively. Finally, the data changes and correlations between the two visits were analyzed.
[0057] 3 Test Instruments: Skin Moisture Loss Tester 4 Data Analysis: (1) Descriptive Statistics: Calculate the mean, standard deviation, maximum value, minimum value, and median of each skin parameter at each time point. (2) Normal Distribution Test: Conduct a normal distribution test on the above means. If the asymptotic significance (two-tailed) value of the normality test > 0.050, then this series of data follows a normal distribution. (3) Difference Analysis: Conduct a difference analysis on the above means. When both sets of data are normally distributed, use a paired T-test to analyze the differences between the two sets of data; when the two sets of data are not both normally distributed, use a rank sum test to analyze the differences between the two sets of data. (4) Judgment Criteria: p > 0.05 represents no statistical difference between the two sets of data, denoted by "n.s."; 0.01 < p < 0.05 represents statistical significance, denoted by "*"; 0.001 < p < 0.01 represents significant difference, denoted by "**"; p < 0.0010 represents extremely significant difference, denoted by "***".
[0058] 5. Test Results: As shown in Table 6 and Figure 1 shown: Table 6 Descriptive Statistical Results and Analysis of Trans-epidermal Water Loss Rate Detection Results
[0059] The test results showed that: After continuously using the test product for 14 days, the results were as Figure 1As shown. The transepidermal water loss rate of the subjects was significantly different from the baseline value (p<0.001), with an improvement rate of 10.46%. Test value: The smaller the TEWL, the better, indicating that the transepidermal water loss of the skin is reduced and the skin barrier is strengthened, indicating that the cosmetic composition has a strong skin repair effect.
[0060] Example 6 Anti-wrinkle test results of cosmetic composition 1. Principles and methods 1. Test Principle Excessive production of free radicals is the main cause of natural skin aging and photoaging. Therefore, scavenging free radicals can delay skin aging and reduce wrinkles. 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH for short) is a stable long-lived free radical. When there is a free radical scavenger, the light absorption of DPPH ethanol solution is weakened due to pairing with its single electron. The degree of fading of DPPH ethanol solution is linearly related to the number of electrons it accepts, which can be used to evaluate the ability of the test sample to scavenge free radicals, that is, the size of the antioxidant activity.
[0061] 2. Test substance: the cosmetic composition prepared in Example 1.
[0062] 3. Test method: Pre-treat the test sample and make a solution. Add the free radical DPPH, prepare multiple tubes of samples, shake well, let stand at room temperature for a period of time, and then test the absorbance at 517nm on a machine to calculate the ability of the sample to scavenge free radicals and calculate the statistical difference P value.
[0063] 4. The test results are as follows: Table 7 Free radical scavenging test results Test items Blank control group Test sample group P-value Free radical scavenging rate (%) 0.00 25.81 <0.05 Test conclusion: The test results are shown in Table 7 and Figure 2 As shown, when the concentration of the test sample was 400 mg / mL, its free radical scavenging rate was 25.81%, and the scavenging rate of the test sample was higher than that of the blank control group, and the statistical difference P value was <0.05, indicating that the test sample had significant anti-wrinkle effect.
[0064] Example 7 Cosmetic composition skin tightening test 1. Principles and methods 1. Test Principle As a highly selective and specific protease, elastase catalyzes the hydrolysis of many amino acids such as glycine, leucine, alanine and other hydroxyl-containing polypeptide bonds, and can decompose elastin in connective tissue proteins. Inhibiting the activity of elastase can effectively slow down the degradation of elastin and reduce skin aging, wrinkles and photoaging.
[0065] 2. Test substance: the cosmetic composition obtained in Example 1, with a concentration of 200 mg / mL.
[0066] 3. Test method: Determination of elastase inhibition rate: Add 90 μL buffer, 30 μL sample diluent of different concentrations and 10 μL substrate to a 96-well plate, mix well and incubate at 25°C for 20 minutes. Then add 20 μL elastase solution and incubate for 10 minutes to detect the absorbance, and calculate the statistical difference P value of the sample's effect on the activity of elastase.
[0067] 2. Test results are as follows Table 8 Elastase inhibition test results Test items Blank control group Test sample group P-value Elastase inhibition rate (%) 0.26 25.52 <0.05 The test results are shown in Table 8 and Figure 3 As shown, when the concentration of the test sample was 200 mg / mL, the elastase inhibition rate was 25.52%, and the inhibition rate of the test sample was higher than that of the blank control group, and the statistical difference P value was <0.05. The test results showed that the test sample had a significant firming effect on the skin.
[0068] Example 8 Skin Soothing Test of Cosmetic Composition 1. Principles and methods Test principle: Hyaluronidase is a participant in type I allergic reactions. Hyaluronidase is strongly correlated with inflammation and allergy. Studies have reported that various drugs that promote the release of histamine from mast cells can regulate hyaluronidase activity. Some anti-allergic drugs have strong inhibitory effects on hyaluronidase activity. Therefore, inhibition of hyaluronidase activity is used as an indicator for studying anti-allergic effects.
[0069] Test substance: the cosmetic composition prepared in Example 1, with a test concentration of 100 mg / mL.
[0070] Hyaluronidase inhibition rate detection method: take the test sample solution and hyaluronidase solution, and incubate at 37℃ for 20min; add 0.1mL of CaCl2 solution and incubate at 37℃ for 20min; add substrate, keep at 37℃ for 40min, and place at room temperature for 10min; add color developer, shake thoroughly, add anhydrous ethanol to 8mL, and place at room temperature for 30min; measure absorbance at 530nm. Calculate the statistical difference P value of the sample's effect on the activity of hyaluronidase.
[0071] 2. Test results Table 9 Hyaluronidase inhibition test results Test items Blank control group Test sample group P-value Hyaluronidase inhibition rate (%) 0.00 48.89 <0.05 The test results are shown in Table 9 and Figure 4As shown, when the concentration of the test sample was 100 mg / mL, the hyaluronidase inhibition rate was 48.89%, and the inhibition rate of the test sample was higher than that of the blank control group, and the statistical difference P value was <0.05. The results showed that the test sample had a significant soothing effect.
[0072] Example 9 Test on moisturizing effect of cosmetic composition 1. Materials and Methods 1. Test Principle Due to the different forces that different moisturizer molecules in cosmetics exert on water molecules, their ability to absorb and retain moisture is slightly different. Testing the moisturizing rate of a moisturizer by a laboratory method (weighing method) can characterize the moisturizing effect of the ingredient.
[0073] 2. Instruments and Materials: Instrument: Constant temperature and humidity chamber Test materials: test substance, water, 10% glycerol solution 3. Test substance: the cosmetic composition obtained in Example 1 4. Blank control: water 5. Standard reference material: 10% glycerol solution 6. Test environment conditions: Test environment temperature is 20 ℃ ± 1 ℃, humidity is 45 ± 2RH% 7. Test method: Weigh the sample, place it in a constant temperature and humidity chamber, weigh the sample at 2, 4, 8, and 24 hours, record the data and calculate the moisture retention rate. Select a reagent with known moisture retention capacity as a standard reference.
[0074] 8. Test result calculation According to the experimental design, the Mt, Yt, and Ht values of each period were measured respectively, and the moisturizing rate was calculated according to the formula: Moisturizing rate P sample = (Mt / M0) × 100% Moisture retention rate Pempty = (Yt / Y0) × 100% Moisture retention rate Pstandard = (Ht / H0) × 100% Relative moisture retention rate % = [P sample - P empty] / [P standard - P empty] × 100% Calculate the relative moisture retention rates of the test samples, blank controls and standard references at each time period.
[0075] 2. Test results are as follows Table 10 Results of moisturizing test of cosmetic compositions
[0076] According to Table 10 and Figure 5It can be seen from the content that according to the relative moisturizing rate and its change curve of the test sample after being placed for 24 hours, it can be obtained that the relative moisturizing rate of the test sample is relatively strong, maintaining between 93.79% and 111.02% within 24 hours.
[0077] Example 10 Test on the applicability of cosmetic composition to sensitive skin 1 Test product Product name: KPC Gastrodia elata repairing and soothing facial mask (provided by Kunming Pharmaceutical Group Health Industry Co., Ltd.) How to use: Open the sample packaging, take out the mask, unfold it, apply it on the facial skin, press lightly to make the mask stick to the skin, and keep it on for 10-20 minutes each time.
[0078] Frequency of use: 4 times a week, 1 tablet each time.
[0079] 2 Testing period: 4 weeks 3 Purpose of testing: The product will be tested on humans for 28 days, and the product’s consumer satisfaction and suitability for sensitive skin will be evaluated through questionnaires filled out by the subjects and records of adverse reactions by dermatologists.
[0080] 4 Test Methods 4.1 Method reference: Comprehensive evaluation is conducted by combining subjective evaluation questionnaires with objective testing methods.
[0081] 4.2 Testing Process The test period was 28 days, and the questionnaires were collected twice (D1, D28). The adverse reactions of the subjects were observed at (D0, D7, D14, D21, D28).
[0082] Table 11 Sensitive skin rating
[0083] 5. Test results are as follows Table 12 Sensitive skin test results
[0084] The test results showed that none of the 30 subjects had adverse skin reactions. The test evaluation results showed that 30 subjects with sensitive skin had no adverse reactions after using the product for 28 days. The results showed that the test product has good safety for subjects with sensitive skin and is suitable for sensitive skin.
[0085] Example 11 Test on skin improvement rate of cosmetic composition 1. Purpose of the test: Use a multifunctional 3D skin imaging analyzer to detect the a* value. The smaller the a* value in the test results, the lighter the red in the skin color.
[0086] 2. Test Results and Analysis: Table 13 Descriptive Statistical Results and Analysis of a* Value Detection Results in Sensitive Skin Test
[0087] All data are expressed as mean ± standard deviation (X±s). a: The improvement rate is the improvement rate relative to that before use. According to Table 13 and Figure 6, the test results show that: after continuous use of the test product for 14 days, the a* value of the subjects is statistically significant compared with the a* value of the baseline value (0.01 < p < 0.05), and the improvement rate is 3.04%, indicating that the red color in the skin color is significantly fading.
[0088] Example 12 Detection and Analysis of EI Value by Multifunctional 3D Skin Imaging Analyzer 1. Test Purpose: Use a multifunctional 3D skin imaging analyzer to test the EI value of the skin. The smaller the skin heme (EI value), the better, indicating that the product has a better effect on relieving skin flushing.
[0089] Table 14 Descriptive Statistical Results and Analysis of EI Value Detection Results
[0090] All data are expressed as mean ± standard deviation (X±s). a: The improvement rate is the improvement rate relative to that before use. According to Table 14 and Figure 7 the results show that: after continuous use of the test product for 14 days, the EI value of the subjects is extremely significantly different from the EI value of the baseline value (p < 0.001), and the improvement rate is 4.29%, indicating that the product has a better effect on relieving skin flushing.
[0091] Example 13 Results of Subject Evaluation 1. Test Product: KPC Gastrodia Elata Soothing and Repairing Skin Mask (provided by Kunming Pharmaceutical Group Health Industry Co., Ltd.).
[0092] 2. Key points of the test method: A total of 30 subjects evaluated the immediate satisfaction with the test product at D0 (immediately after using the product), and evaluated the product effect at D14. The results of the immediate satisfaction with the use of the test product are shown in the following table: Scoring standard (5-point system) The results of the immediate satisfaction with the use of the test product are shown in the following table: Scoring standard (5-point system): 1 point is dissatisfied, 2 points are relatively dissatisfied, 3 points are average, 4 points are satisfied, and 5 points are very satisfied. The statistical score ≥ 3 points is the effective number of people who choose to be satisfied; Satisfaction = the effective number of people who choose to be satisfied ÷ the effective number of test participants × 100%; the results of the product effect evaluation are shown in the following table: Scoring standard (4-point system): 0 points: no improvement; 1 point: slight improvement; 2 points: moderate improvement; 3 points: obvious improvement. Statistical percentage = the number of people with each score ÷ the effective number of test participants × 100%.
[0093] 3. Test results are shown in the following table: Table 15 Evaluation results of immediate use satisfaction of the test products
[0094] Table 16 Results of product effect evaluation by subject D14
[0095] Test results: According to Tables 15, 16 and Figure 8 and Fig. 9 The results showed that the subjects had a high degree of acceptance and satisfaction with the product, and that the product significantly improved the subjects' overall skin condition.
[0096] Example 14 Detection test of diethylene glycol in cosmetic composition 1. Test product: the cosmetic composition prepared in Example 1 2. Main instruments and equipment: gas chromatograph, hydrogen flame ionization detector; balance; gas chromatograph-mass spectrometer, etc.
[0097] 3. Summary of detection method: (1) After sample extraction, the sample is analyzed by gas chromatography, qualitative analysis is performed based on retention time, quantitative analysis is performed based on peak area, and the content is calculated using the standard curve method. If necessary, positive results can be further confirmed by gas chromatography-mass spectrometry. The instrument detection limit of diethylene glycol in this method is 0.3 mg / L, the instrument quantitative limit is 0.9 mg / L, and the method detection concentration is 0.003%.
[0098] (2) Preparation of standard series solutions: Take the diethylene glycol standard stock solution and use anhydrous ethanol to prepare a series of diethylene glycol standard solutions with concentrations of 1 μg / mL, 2 μg / mL, 4 μg / mL, 8 μg / mL, 10 μg / mL, and 16 μg / mL.
[0099] (3) Sample preparation: Weigh 1g of sample (accurate to 0.001g) into a 100mL glass test tube, add appropriate amount of anhydrous ethanol to the scale, vortex to mix and then test.
[0100] (4) Chromatographic conditions: Chromatographic column: DB-Wax 52 CB (50m×0.25mm×0.2μm), or equivalent chromatographic column; Column temperature program: starting temperature is 160°C, maintained for 10 min, increased to 220°C at a rate of 20°C / min, maintained for 4 min; Inlet temperature: 230°C; Detector temperature: 250°C; Carrier gas: nitrogen (99.999%), flow rate: 1mL / min; Hydrogen flow rate: 40mL / min; Air flow rate: 400mL / min; Tail gas nitrogen flow rate: 30mL / min; Injection mode: split injection, split ratio: 5:1; Injection volume: 1.0μL.
[0101] (5) Determination: Under the chromatographic conditions of "(4)", sample the standard series solutions of "(2)" and perform gas chromatography analysis. Draw a standard curve with the concentration of the standard series solutions as the horizontal axis and the peak area as the vertical axis. Sample the solution to be tested under "(3)" and perform gas chromatography analysis. Qualitative analysis is performed based on the retention time, and the peak area is measured. The concentration of diethylene glycol in the solution to be tested is obtained based on the standard curve.
[0102] 4. The test results are shown in the following table Table 17 Diethylene glycol test results Test items unit Test Method Test Results Detection limit Index requirements Single item evaluation Diethylene glycol % See above for details Not detected 0.003 Do not add conform to The test results showed that no diethylene glycol was detected in the test product, which met the requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition).
[0103] The above is only a preferred embodiment of the present invention, and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment, it is not used to limit the present invention. Any technician familiar with this patent can make some changes or modify the technical contents suggested above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the solution of the present invention.
Claims
1. A cosmetic composition, characterized in that The cosmetic composition comprises the following components by mass percentage: 1-3% glycerin, 1-2% dipropylene glycol, 0.8-1.2% beetroot extract, 0.3-0.6% preservative-free, 0.3-0.6% polydimethylsiloxane, 0.1-0.3% allantoin, 0.1-0.3% 1,2-hexanediol, 0.1-0.3% cell revitalizing biopeptide, 0.1-0.2% polyglycerol-10 oleate, 0.1-0.2% yeast black tea fermentation product, 0.1-0.2% p-hydroxyacetophenone, 0.1-0.13% jojoba seed oil, 0.1-0.13% carbomer, 0.1-0.13% gastrodia elata extract, 0.8-0.11% dipotassium glycyrrhizinate, 0.9-0.11% hydrolyzed sclerotium gum, and 0.9-0.11% purple snow ginseng nourishing oil.
2. The cosmetic composition according to claim 1, characterized in that The cosmetic composition further comprises the following components, measured by mass percentage: 0.06-0.07% tromethamine, 0.03-0.05% hydroxyethyl cellulose, 0.006-0.01% rosewood oil, 0.0006-0.0015% giant kelp extract, 0.0006-0.0015% ambergris fruit extract, 0.0006-0.0015% jasmine leaf cell extract, 0.0006-0.0015% light peptide, 0.0006-0.0015% cordyceps extract, 0.0006-0.0015% hyaluronic acid, 0.0006-0.0015% sea fennel callus culture filtrate, and 0.0006-0.0015% Centella asiatica extract.
3. The cosmetic composition according to claim 2, characterized in that The cosmetic composition comprises the following components by weight percentage: 92.7552% water; 2% glycerin; 1.5% dipropylene glycol; 1% beetroot extract; 0.5% preservative-free; 0.5% polydimethylsiloxane; 0.2% allantoin; 0.2% 1,2-hexanediol; 0.15% cell revitalizing biopeptide; 0.15% polyglyceryl-10 oleate; Saccharomyces black tea fermentation product 0.15%, p-hydroxyacetophenone 0.15%, jojoba seed oil 0.11%, carbomer 0.11%, CMZZ-Gastrodia extract 0.11%, dipotassium glycyrrhizinate 0.101%, hydrolyzed sclerotium gum 0.1%, purple snow ginseng oil 0.1%, tromethamine 0.065%, hydroxyethyl cellulose 0.04%, rosewood oil 0.008%, giant kelp extract 0.0001%, coffee sunflower fruit extract 0.0001%, jasmine leaf cell extract 0.0001%, time peptide 0.0001%, cordyceps extract 0.0001%, hyaluronic acid 0.0001%, sea fennel callus culture filtrate 0.0001%, Centella asiatica extract 0.0001%.
4. The cosmetic composition according to any one of claims 1 to 3, for preparing cosmetics or cosmetic compositions for dermatology, comprising: Use in solutions, gels, hydrogels, gel-creams, gel-pastes, creams, ointments, temporary mask preparations, patches and in general skin cosmetics (makeup), which can be used in the formulation of cosmetic products that can be used to prevent and correct the characteristic symptoms of skin aging produced by endogenous and exogenous stress factors.
5. A skin-penetrating facial mask comprising a Gastrodia elata extract, characterized in that: The skin-penetrating facial mask comprises the cosmetic composition according to any one of claims 1 to 3, an auxiliary material acceptable in skin care products, and a solvent acceptable in skin care products.
6. The skin-penetrating facial mask containing Gastrodia elata extract according to claim 5, characterized in that: The skin-penetrating mask comprises the following components by weight percentage: 1-3% glycerin, 1-2% dipropylene glycol, 0.8-1.2% beetroot extract, 0.3-0.6% preservative-free, 0.3-0.6% polydimethylsiloxane, 0.1-0.3% allantoin, 0.1-0.3% 1,2-hexanediol, 0.1-0.3% cell-rejuvenating biopeptide, 0.1-0.2% polyglycerol-10 oleate, 0.1-0.2% yeast black tea fermentation product, 0.1-0.2% p-hydroxyacetophenone, 0.1-0.13% jojoba seed oil, 0.1-0.13% carbomer, 0.1-0.13% gastrodia elata extract, 0.8-0.11% dipotassium glycyrrhizate, 0.9-0.11% hydrolyzed sclerotium gum, and 0.9-0.11% purple snow ginseng nourishing oil.
7. The skin-penetrating facial mask containing Gastrodia elata extract according to claim 6, characterized in that: The skin-penetrating facial mask further comprises the following components, measured by weight percentage: 0.06-0.07% tromethamine, 0.03-0.05% hydroxyethyl cellulose, 0.006-0.01% rosewood oil, 0.0006-0.0015% giant kelp extract, 0.0006-0.0015% sunflower seed extract, 0.0006-0.0015% jasmine leaf cell extract, 0.0006-0.0015% light peptide, 0.0006-0.0015% cordyceps extract, 0.0006-0.0015% hyaluronic acid, 0.0006-0.0015% sea fennel callus culture filtrate, and 0.0006-0.0015% Centella asiatica extract.
8. The skin-penetrating facial mask containing Gastrodia elata extract according to claim 7, characterized in that: The skin-penetrating mask contains the following components by weight percentage: 92.7552% water; 2% glycerin, 1.5% dipropylene glycol; 1% beetroot extract; 0.5% preservative-free; 0.5% polydimethylsiloxane; 0.2% allantoin; 0.2% 1,2-hexanediol; 0.15% cell revitalizing biopeptide; polyglycerol-10 Oleate 0.15%, Saccharomyces black tea fermentation product 0.15%, p-hydroxyacetophenone 0.15%, jojoba seed oil 0.11%, carbomer 0.11%, CMZZ-Gastrodia extract 0.11%, dipotassium glycyrrhizinate 0.101%, hydrolyzed sclerotium gum 0.1%, purple snow ginseng oil 0.1%, tromethamine 0.065%, hydroxyethyl cellulose 0.04%, rosewood oil 0.008%, giant kelp extract 0.0001%, coffee sunflower fruit extract 0.0001%, jasmine leaf cell extract 0.0001%, time peptide 0.0001%, cordyceps extract 0.0001%, hyaluronic acid 0.0001%, sea fennel callus culture filtrate 0.0001%, Centella asiatica extract 0.0001%.
9. A method for preparing a cosmetic composition, characterized in that: The preparation method comprises the following steps: (1) Add the raw materials of phase A into the emulsifying pot, raise the temperature to 80-85℃ while stirring, stir for 8-10 minutes to mix evenly, and keep warm for 10 minutes. (2) Cool the product obtained in step (1) to 45-50°C, add the raw materials of phase B and phase C, and stir for 8-10 minutes; (3) Cooling the product obtained in step (2) to below 38°C, discharging the product, and allowing it to stand to obtain a finished product; The raw materials described in the preparation method and the mass percentage content of each raw material are: Phase A: Water 92.7552%; Glycerin 99.5% USP, 2%; Dipropylene glycol 1.5%; Dimethicone 0.5%; Allantoin 0.2%; Polyglyceryl-10 Oleate 0.15%; Parahydroxyacetophenone 0.15%, Jojoba Seed 0.11%, Carbomer 0.11%, Hydrolyzed Sclerotium Gum 0.1%, Hydroxyethylcellulose 0.04%; Phase B: Beetroot extract 1%, Cell Rejuvenating Biopeptide 0.15%, Saccharomyces cerevisiae black tea fermentation product 0.15%, Gastrodia elata extract 0.11%, Dipotassium glycyrrhizinate 0.101%, Purple Snow Ginseng Oil 0.1%, Sea Fennel Callus Culture Filtrate 0.0001%; Phase C: No added preservatives 0.5%, 1,2-hexanediol 0.2%, tromethamine 0.065%, rosewood oil 0.008%, giant kelp extract 0.0001%, ambrette fruit extract 0.0001%, jasmine leaf cell extract 0.0001%, time peptide 0.0001%, cordyceps extract 0.0001%, hyaluronic acid 0.0001%, Centella asiatica extract 0.0001%.
10. The preparation method according to claim 9, characterized in that: The temperature in step (1) is 83°C and the stirring time is 9 minutes; the temperature in step (2) is 48°C and the stirring time is 10 minutes; the temperature in step (3) is 10-36°C and the standing time is 12-48 hours.
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