High-precision detection device and method for dendrophenol

Abstract

The invention belongs to the technical field of dendrophenol detection, and particularly relates to a high-precision dendrophenol detection device and method. The invention provides a dendrophenol high-precision detection and extraction device, which comprises: an outer sleeve tank, which is arranged at the outermost part and has an opening at the top, and a sealing cover is arranged at the opening; the inner sleeve tank is arranged in the outer sleeve tank, an interlayer space is formed between the outer sleeve tank and the inner sleeve tank, the inner sleeve tank is rotationally arranged in the outer sleeve tank, the top of the inner sleeve tank is open, the bottom of the inner sleeve tank is connected with a discharging pipe, and the discharging pipe penetrates out of the bottom of the outer sleeve tank; the multifunctional controller is arranged above the outer sleeve tank, and the multifunctional controller comprises an adjusting rotating plate, a cleaning mechanism and a smashing mechanism; the invention provides a high-precision detection method for dendrophenol. According to the high-precision dendrophenol detection device and method provided by the invention, manual operation is replaced, various devices are integrated, and not only can the extraction efficiency be improved, but also the detection precision can be improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of dendrobium phenol detection, and specifically refers to a high-precision detection device and method for dendrobium phenol. Background Art

[0002] Dendrobium has a long history of medicinal use, and its medicinal ingredients are both rich and balanced, which is conducive to the treatment of various diseases. It is clinically used to treat chronic pharyngitis, gastrointestinal diseases, ophthalmic diseases, thrombotic occlusive diseases, diabetes, arthritis, and cancer. Treatment or adjuvant therapy. In addition, Dendrobium can also be used in food processing, can add natural sweetness and fragrance to juice and tea, improve nutritional value, and can be used in the production of baked goods such as bread and cakes to increase the taste and flavor of food. In addition, it is also widely used in the fields of condiments and health products. Dendrobium extract is a natural additive, which is healthy and safe, does not contain the advantages of chemical additives, and also has health functions such as anti-oxidation, anti-inflammatory, and anti-fatigue. The existing dendrobium phenol extraction method is relatively cumbersome, requires the use of a variety of large-scale equipment, is costly, and the extraction steps are complicated, and it relies more on manual operation, resulting in low detection accuracy and efficiency. Summary of the invention

[0003] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a high-precision detection device and method for dendrobium phenol, which replaces manual operation and integrates multiple devices into one, thereby improving both the extraction efficiency and the detection accuracy.

[0004] The technical solution adopted by the present invention is as follows: On the one hand, the present invention provides a high-precision detection device for dendrobium phenol, comprising:

[0005] The outer can is arranged at the outermost part, has an opening at the top and a sealing cover at the opening;

[0006] The inner tank is arranged inside the outer tank, and a sandwich space is provided between the outer tank and the inner tank. The inner tank is rotatably arranged inside the outer tank. The inner tank has an opening at the top and a feed pipe connected to the bottom. The feed pipe passes through the bottom of the outer tank.

[0007] A multifunctional controller is arranged above the outer sleeve tank, and the multifunctional controller includes an adjusting rotating plate, a cleaning mechanism and a crushing mechanism. The adjusting rotating plate can be rotatably arranged, and a motor for controlling its rotation is connected to it. The cleaning mechanism and the crushing mechanism are arranged on the adjusting rotating plate. The crushing mechanism includes a first telescopic cylinder, a first cover plate and a crushing shaft. The first telescopic cylinder is arranged on the adjusting rotating plate, and the first cover plate is connected to the first telescopic cylinder. The crushing shaft is rotatably arranged on the first cover plate, and a motor is connected to the crushing shaft. A crushing knife is arranged on the crushing shaft; the cleaning mechanism includes a second telescopic cylinder, a second cover plate and a cleaning pipe. The second telescopic cylinder is arranged on the adjusting rotating plate, and the second cover plate is arranged on the second telescopic cylinder. The cleaning pipe is arranged on the second cover plate, and a cleaning nozzle is arranged on the cleaning pipe. The first cover plate and the second cover plate are all adapted to the filling mouth of the inner sleeve tank.

[0008] Furthermore, the crushing blade includes a large crushing blade, a small crushing blade and an inclined crushing blade, and the inclined crushing blade includes a crushing blade inclined downward and a crushing blade inclined upward.

[0009] Furthermore, the cleaning tube is rotatably arranged, and the second cover plate is provided with a cleaning drive mechanism, including a cleaning shell, a cleaning gear, a cleaning gear ring and a water inlet pipe. The cleaning tube is rotatably arranged on the cleaning shell, the cleaning gear ring is connected to the cleaning tube, the cleaning gear and the cleaning gear ring are meshed, the water inlet pipe penetrates into the cleaning shell and is rotatably connected to the cleaning tube, and the cleaning gear is connected to a driving motor.

[0010] Furthermore, the interlayer space includes an upper space and a lower space, and the upper space and the lower space are separated by a partition, the partition is located at the feed pipe, the partition is provided with an opening, the feed pipe passes through the partition and is rotatably connected to the partition, and a rotating seal is provided at the connection, a heating mechanism is provided in the upper space, and the upper space is connected with a water inlet pipe and a drain pipe; a solenoid valve is provided on the feed pipe, and a drain port is also provided at the bottom of the inner tank, a solenoid valve is provided in the drain port, a filter is provided in the drain port, the drain port is connected to the upper space, the upper space is connected to a reflux port, the reflux port is connected to a reflux pipe, the reflux pipe is a hose, the reflux pipe is connected to a reflux box, the reflux box is connected to a reflux pump, the reflux pipe is connected to the cover, and passes through the cover so that the reflux liquid can flow into the inner tank again.

[0011] Furthermore, a rotary drive mechanism is provided in the lower space, and the rotary drive mechanism includes a connecting gear ring and a rotary drive gear, the connecting gear ring is fixedly connected to the outside of the discharge pipe, the rotary drive gear and the connecting gear ring are meshed and connected, and the rotary drive gear is connected to a drive motor.

[0012] Furthermore, the inner wall of the inner tank is provided with crushing teeth, and the rotation of the inner tank is controlled by a rotary drive mechanism. When the crushing shaft crushes the material, the crushing teeth on the inner wall assist in crushing, and by setting the rotation directions of the two in opposite directions, relative motion is formed, which can improve the crushing effect.

[0013] Furthermore, the sealing cover is provided with a liquid injection connection port, and the liquid injection connection port is connected to a liquid injection pipeline for injecting enzymatic solution or clean water.

[0014] On the other hand, the present invention provides a high-precision detection method for dendrobium phenol, comprising the following steps:

[0015] Step 1: Wash and cut the dendrobium into small pieces, put the dendrobium into a container and heat it at 50 degrees for 30 minutes;

[0016] Step 2: preparing an enzymatic hydrolysis solution, firstly putting the enzyme into low temperature water to dissolve it to form an enzymatic hydrolysis solution, adding the enzymatic hydrolysis solution into a container of dendrobium, enzymolyzing the dendrobium, the enzymatic hydrolysis temperature is 40 degrees, and the enzymatic hydrolysis time is 2 hours;

[0017] Step 3: After enzymolysis, take out the dendrobium and crush it. During the crushing process, add an appropriate amount of clean water, the water level is 1 / 3 of the volume of the dendrobium, crush the dendrobium, and generate a crushed dendrobium solution;

[0018] Step 4: Put the crushed dendrobium solution into the enzymatic solution again for secondary dissolution, the enzymatic temperature is 40 degrees, and the enzymatic time is 3 hours;

[0019] Step 5: Filter out the dissolved solution and filter it through a filter with a 300-mesh filter;

[0020] Step 6: Add a purifying agent to the dissolved solution to remove impurities, and then allow the solution to settle. After 12 hours, extract the essence extract.

[0021] Step seven, the extract is subjected to ultra-high performance liquid chromatography-electrochemical detection method to detect the content of dendrobium phenol.

[0022] Furthermore, in step six, sedimentation can be carried out by centrifugal sedimentation.

[0023] The beneficial effects achieved by the present invention using the above structure are as follows: This scheme provides a high-precision detection device and method for dendrobium phenol. By setting up an inner and outer double extraction tank, the dendrobium can be heated in water during extraction, thereby destroying the components in the dendrobium; a multi-functional controller is set to achieve replaceable use, and the dendrobium is crushed and the tank body is cleaned respectively; a rotatable inner sleeve tank is set, which can not only improve the crushing effect by generating a reverse rotation force, but also achieve rapid sedimentation and extraction of the dissolved liquid through centrifugal action, thereby improving the overall extraction effect. This scheme also discloses a high-precision detection method for dendrobium phenol. Through an efficient extraction method and a high-precision detection method, the extraction and utilization of dendrobium phenol are improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a three-dimensional diagram of the overall structure of this scheme;

[0025] Figure 2 A three-dimensional diagram of the jacket tank of this scheme;

[0026] Figure 3 It is a schematic diagram of the internal structure of the outer jacket tank;

[0027] Figure 4 It is a three-dimensional diagram of the inner sleeve tank;

[0028] Figure 5 It is a three-dimensional diagram of the crushing mechanism;

[0029] Figure 6 A three-dimensional diagram of a cleaning mechanism;

[0030] Figure 7 Schematic diagram of the internal structure of the cleaning drive mechanism.

[0031] Among them, 1. outer can; 2. inner can; 3. multi-function controller; 4. adjusting rotating plate; 5. cleaning mechanism; 6. crushing mechanism; 7. first telescopic cylinder; 8. first cover plate; 9. crushing shaft; 10. crushing knife; 11. second telescopic cylinder; 12. second cover plate; 13. cleaning pipe; 14. cleaning nozzle; 15. large crushing knife; 16. small crushing knife; 17. inclined crushing knife; 18. cleaning drive mechanism; 19. cleaning shell; 20. Cleaning gear; 21, cleaning gear ring; 22, water inlet pipe; 23, upper space; 24, lower space; 25, partition; 26, water inlet pipe; 27, drain pipe; 28, drain port; 29, reflux port; 30, reflux pipe; 31, reflux box; 32, reflux pump; 33, rotary drive mechanism; 34, connecting gear ring; 35, rotary drive gear; 36, crushing tooth; 37, injection connection port; 38, injection pipeline; 39, capping; 40, discharge pipe

[0032] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0034] In the description of the present invention, it should be understood that terms such as “upper”, “lower”, “front”, “back”, “left”, “right”, “top”, “bottom”, “inside” and “outside” indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0035] The technical solution adopted by the present invention is as follows: On the one hand, the present invention provides a high-precision detection device for dendrobium phenol, comprising:

[0036] The outer shell 1 is arranged at the outermost part, the top of which is open, and a sealing cover 39 is arranged at the opening;

[0037] The inner tank 2 is arranged inside the outer tank 1, and a sandwich space is provided between the outer tank 1 and the inner tank 2. The inner tank 2 is rotatably arranged inside the outer tank 1. The inner tank 2 has an opening at the top and a feed pipe 40 connected to the bottom. The feed pipe 40 passes through the bottom of the outer tank 1.

[0038] A multifunctional controller 3 is arranged above the outer sleeve tank 1, and the multifunctional controller 3 includes an adjusting rotating plate 4, a cleaning mechanism 5 and a crushing mechanism 6. The adjusting rotating plate 4 can be rotatably arranged, and a motor for controlling its rotation is connected thereto. The cleaning mechanism 5 and the crushing mechanism 6 are arranged on the adjusting rotating plate 4. The crushing mechanism 6 includes a first telescopic cylinder 7, a first cover plate 8 and a crushing shaft 9. The first telescopic cylinder 7 is arranged on the adjusting rotating plate 4, and the first cover plate 8 is connected to the first telescopic cylinder 7. The crushing shaft 9 is rotatably arranged on the first cover plate 8, and a motor is connected to the crushing shaft 9. A crushing knife 10 is arranged on the crushing shaft 9; the cleaning mechanism 5 includes a second telescopic cylinder 11, a second cover plate 12 and a cleaning pipe 13. The second telescopic cylinder 11 is arranged on the adjusting rotating plate 4, and the second cover plate 12 is arranged on the second telescopic cylinder 11. The cleaning pipe 13 is arranged on the second cover plate 12, and a cleaning nozzle 14 is arranged on the cleaning pipe 13. The first cover plate 8 and the second cover plate 12 are all adapted to the filling mouth of the inner sleeve tank 2.

[0039] The crushing blade 10 includes a large crushing blade 15, a small crushing blade 16 and an inclined crushing blade 17. The inclined crushing blade 17 includes a crushing blade inclined downward and a crushing blade inclined upward.

[0040] The cleaning tube 13 is rotatably arranged, and a cleaning drive mechanism 18 is provided on the second cover plate 12, including a cleaning shell 19, a cleaning gear 20, a cleaning gear ring 21 and a water inlet pipe 26. The cleaning tube 13 is rotatably arranged on the cleaning shell 19, and the cleaning gear ring 21 is connected to the cleaning tube 13. The cleaning gear 20 is meshed with the cleaning gear ring 21. The water inlet pipe 26 penetrates into the cleaning shell 19 and is rotatably connected to the cleaning tube 13. The cleaning gear 20 is connected to a driving motor.

[0041] The interlayer space includes an upper space 23 and a lower space 24, and the upper space 23 and the lower space 24 are separated by a partition 25, and the partition 25 is located at the feed pipe 40, and the partition 25 is provided with an opening, and the feed pipe 40 passes through the partition 25 and is rotatably connected to the partition 25, and a rotary seal is provided at the connection, and a heating mechanism is provided in the upper space 23, and the upper space 23 is connected to a water inlet pipe 22 and a drain pipe 27; the feed pipe 40 is provided with a solenoid valve, and the inner sleeve tank 2 A drain port 28 is also provided at the bottom, a solenoid valve is provided in the drain port 28, a filter screen is provided in the drain port 28, the drain port 28 is connected to the upper space 23, the upper space 23 is connected to a reflux port 29, the reflux port 29 is connected to a reflux pipe 30, the reflux pipe 30 is a hose, the reflux pipe 30 is connected to a reflux box 31, the reflux box 31 is connected to a reflux pump 32, the reflux pipe 30 is connected to a sealing cover 39, and passes through the sealing cover 39, so that the reflux liquid can flow into the inner sleeve tank 2 again.

[0042] A rotary drive mechanism 33 is provided in the lower space 24 , and the rotary drive mechanism 33 includes a connecting gear ring 34 and a rotary drive gear 35 . The connecting gear ring 34 is fixedly connected to the outside of the discharge pipe 40 , and the rotary drive gear 35 is meshedly connected to the connecting gear ring 34 . The rotary drive gear 35 is connected to a drive motor.

[0043] The inner wall of the inner tank 2 is provided with crushing teeth 36, and the rotation of the inner tank 2 is controlled by the rotary drive mechanism 33. When the crushing shaft 9 crushes the material, the crushing teeth 36 on the inner wall assist in crushing, and by setting the rotation directions of the two in opposite directions, a relative motion is formed, which can improve the crushing effect.

[0044] The sealing cover 39 is provided with a liquid injection connection port 37 , and the liquid injection connection port 37 is connected to a liquid injection pipeline 38 for injecting an enzymatic solution or clean water.

[0045] On the other hand, the present invention provides a high-precision detection method for dendrobium phenol, comprising the following steps:

[0046] Step 1: Wash and cut the dendrobium into small pieces, put the dendrobium into a container and heat it at a temperature of 50 degrees for 30 minutes; in this embodiment, the dendrobium is first placed in the inner tank 2, and water is injected into the outer tank 1, and the specific water injection position is the upper space 23 of the interlayer space, so as to achieve the effect of water insulation heating;

[0047] Step 2: preparing an enzymatic hydrolysis solution, firstly putting the enzyme into low temperature water to dissolve it to form an enzymatic hydrolysis solution, adding the enzymatic hydrolysis solution into a container of dendrobium, enzymolyzing the dendrobium, the enzymatic hydrolysis temperature is 40 degrees, and the enzymatic hydrolysis time is 2 hours;

[0048] Step 3, after enzymolysis, the dendrobium is taken out and crushed; in this embodiment, the drain port 28 of the inner sleeve tank 2 is opened to discharge the enzymolysis solution, and the solution enters the upper space 23; during the crushing process, an appropriate amount of clean water is added, and the water level is 1 / 3 of the volume position of the dendrobium, and the dendrobium is crushed to generate a crushed dendrobium solution; in this embodiment, the rotating plate 4 is operated and adjusted to rotate, so that the crushing mechanism 6 docks with the inner sleeve tank 2, and the first telescopic cylinder 7 is controlled to push the crushing shaft 9 to the inner sleeve tank 2, and the motor is started to drive the crushing knife to crush. By setting multiple crushing knives of different lengths and angles, the crushing efficiency and accuracy are improved, thereby improving the extraction efficiency. At the same time, the rotary drive mechanism 33 is started, and the motor drives the rotary drive gear 35 to rotate, thereby driving the connecting gear ring 34 to rotate, thereby driving the inner sleeve tank 2 to rotate, and the inner sleeve tank 2 and the crushing shaft 9 rotate in the opposite direction. Under the dual action of the crushing teeth 36 on the inner wall of the inner sleeve tank 2 and the crushing knife on the crushing shaft 9, the crushing effect of the dendrobium is further improved.

[0049] Step 4: Put the crushed dendrobium solution into the enzymatic solution again for secondary dissolution, the enzymatic temperature is 40 degrees, and the enzymatic time is 3 hours; in this embodiment, the enzymatic solution flows back from the reflux pipe 30 to the reflux box 31, and then pumped into the inner sleeve tank 2 again for enzymatic hydrolysis;

[0050] Step 5: Filter out the dissolved liquid and use a filter screen with a mesh size of 300. In this embodiment, the dissolved liquid is discharged from the inner tank 2 into the upper space 23, filtered during discharge, and then temporarily stored in the reflux box 31 through the reflux port 29, and then the solenoid valve of the feed pipe is opened to discharge the dendrobium residue, and the cleaning mechanism 5 is controlled to enter the inner tank 2 for flushing.

[0051] Step 6: Add a purifying agent to the dissolved solution to remove impurities, and then allow the solution to settle. After 12 hours, extract the essence extract.

[0052] Step seven, the extract is subjected to ultra-high performance liquid chromatography-electrochemical detection method to detect the content of dendrobium phenol.

[0053] In step six, the sedimentation can be carried out by centrifugal sedimentation. In this solution, the dissolved liquid is pumped from the reflux box 31 into the inner tank 2, and the inner tank 2 is controlled to rotate, thereby accelerating the sedimentation efficiency.

[0054] When used specifically, the dendrobium is cleaned and cut into small pieces, and the dendrobium is placed in the inner sleeve tank 2, and water is injected into the outer sleeve tank 1, and the specific water injection position is the upper space 23 of the interlayer space, and then heated, the heating temperature is 50 degrees, and the heating time is 30 minutes; to prepare the enzymatic hydrolyzate, first put the enzyme into low-temperature water, dissolve it to form an enzymatic hydrolyzate solution, add the enzymatic hydrolyzate solution into the inner sleeve tank 2, enzymolyze the dendrobium, the enzymatic hydrolysis temperature is 40 degrees, and the enzymatic hydrolysis time is 2 hours; after the enzymatic hydrolysis, the drain port 28 of the inner sleeve tank 2 is opened to discharge the enzymatic hydrolyzate solution , the solution enters the upper space 23 and is stored in the reflux box 31 through the reflux pipe 30; clean water is injected into the inner sleeve tank 2, and the water level is adjusted by operating the rotating plate 4 to rotate, so that the crushing mechanism 6 docks with the inner sleeve tank 2, and the first telescopic cylinder 7 is controlled to push the crushing shaft 9 to the inner sleeve tank 2, and the motor is started to drive the crushing knife to crush. By setting multiple crushing knives of different lengths and angles, the crushing efficiency and accuracy are improved, thereby improving the extraction efficiency. At the same time, the rotary drive mechanism 33 is started, and the motor drives the rotary drive gear 35 to rotate, thereby driving The dynamic connection tooth ring 34 rotates, thereby driving the inner sleeve tank 2 to rotate, and the inner sleeve tank 2 and the crushing shaft 9 rotate in the opposite direction. Under the dual action of the crushing teeth 36 on the inner wall of the inner sleeve tank 2 and the crushing knife on the crushing shaft 9, the crushing effect of the dendrobium is further improved; then the enzymatic solution is pumped from the reflux box 31 to the inner sleeve tank 2 again for enzymatic hydrolysis, the enzymatic hydrolysis temperature is 40 degrees, and the enzymatic hydrolysis time is 3 hours; the dissolved liquid is discharged from the inner sleeve tank 2 to the upper space 23, filtered during discharge, and then temporarily stored in the reflux box 31 through the reflux port 29; Then open the solenoid valve of the discharge pipe to discharge the dendrobium residue, and control the cleaning mechanism 5 to enter the inner sleeve tank 2 for flushing, start the motor to drive the cleaning gear 20 to rotate, and then drive the cleaning gear ring 21 to rotate, and then drive the cleaning tube 13 to rotate, and then rotate during spraying to achieve a rotary spray effect and clean the residue in the tank; pump the dissolved liquid into the inner sleeve again, and control the rotation of the inner sleeve tank 2 to achieve centrifugal sedimentation, extract the essence extract, and detect the dendrobium phenol content by ultra high performance liquid chromatography-electrochemical detection method.

[0055] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0056] The present invention and its embodiments are described above, and such description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.

Claims

1. A high-precision detection device for dendrobium phenol, characterized in that: include: An outer can (1) is arranged at the outermost part, has an opening at the top and a sealing cover at the opening; The inner tank (2) is arranged inside the outer tank (1), and an interlayer space is provided between the outer tank (1) and the inner tank (2). The inner tank (2) is rotatably arranged inside the outer tank (1). The inner tank (2) has an opening at the top and a discharge pipe connected to the bottom. The discharge pipe passes through the bottom of the outer tank (1); A multifunctional controller (3) is arranged above the outer casing (1). The multifunctional controller (3) comprises an adjusting rotating plate (4), a cleaning mechanism (5) and a crushing mechanism (6). The adjusting rotating plate (4) is rotatably arranged and is connected to a motor for controlling its rotation. The cleaning mechanism (5) and the crushing mechanism (6) are arranged on the adjusting rotating plate (4). The crushing mechanism (6) comprises a first telescopic cylinder (7), a first cover plate (8) and a crushing shaft (9). The first telescopic cylinder (7) is arranged on the adjusting rotating plate (4). The first cover plate (8) is connected to the first telescopic cylinder (7). The crushing shaft (9) The first cover plate (8) is rotatably mounted on the pulverizing shaft (9), a motor is connected to the pulverizing shaft (9), and a pulverizing blade (10) is arranged on the pulverizing shaft (9); the cleaning mechanism (5) comprises a second telescopic cylinder (11), a second cover plate (12) and a cleaning pipe (13), the second telescopic cylinder (11) is arranged on the adjusting rotating plate (4), the second cover plate (12) is arranged on the second telescopic cylinder (11), the cleaning pipe (13) is arranged on the second cover plate (12), a cleaning nozzle (14) is arranged on the cleaning pipe (13), and the first cover plate (8) and the second cover plate (12) are all adapted to the filling port of the inner sleeve tank (2).

2. A high-precision detection device for dendrobium phenol according to claim 1, characterized in that: The cleaning tube (13) is rotatably arranged. The second cover plate (12) is provided with a cleaning drive mechanism (18), comprising a cleaning shell (19), a cleaning gear (20), a cleaning gear ring (21) and a water inlet pipe (26). The cleaning tube (13) is rotatably arranged on the cleaning shell (19). The cleaning gear (20) is connected to the cleaning tube (13). The cleaning gear (20) is meshed with the cleaning gear ring (21). The water inlet pipe (26) penetrates into the cleaning shell (19) and is rotatably connected to the cleaning tube (13). The cleaning gear (20) is connected to a driving motor.

3. A high-precision detection device for dendrobium phenol according to claim 1, characterized in that: The interlayer space comprises an upper space (23) and a lower space (24), the upper space (23) and the lower space (24) are separated by a partition (25), the partition (25) is located at the feed pipe, an opening is provided on the partition (25), the feed pipe passes through the partition (25), and is rotatably connected to the partition (25), and a rotary seal is provided at the connection, a heating mechanism is provided in the upper space (23), and the upper space (23) is connected to a water inlet pipe (22) and a drain pipe (27); the feed pipe is provided with a solenoid valve, and the The bottom of the inner tank (2) is also provided with a liquid discharge port (28), a solenoid valve is provided in the liquid discharge port (28), a filter screen is provided in the liquid discharge port (28), the liquid discharge port (28) is connected to the upper space (23), the upper space (23) is connected to a reflux port (29), the reflux port (29) is connected to a reflux pipe (30), the reflux pipe (30) is a hose, the reflux pipe (30) is connected to a reflux box (31), the reflux box (31) is connected to a reflux pump (32), the reflux pipe (30) is connected to the sealing cover and passes through the sealing cover.

4. A high-precision detection device for dendrobium phenol according to claim 3, characterized in that: A rotary drive mechanism (33) is provided in the lower space (24), and the rotary drive mechanism (33) comprises a connecting gear ring (34) and a rotary drive gear (35). The connecting gear ring (34) is fixedly connected to the outside of the discharge pipe, the rotary drive gear (35) is meshedly connected with the connecting gear ring (34), and the rotary drive gear (35) is connected to a drive motor.

5. The high-precision detection device for dendrobium phenol according to claim 3, characterized in that: The inner wall of the inner casing tank (2) is provided with crushing teeth (36).

6. A high-precision detection device for dendrobium phenol according to claim 1, characterized in that: The crushing blade (10) comprises a large crushing blade (15), a small crushing blade (16) and an inclined crushing blade (17), and the inclined crushing blade (17) comprises a crushing blade inclined downward and a crushing blade inclined upward.

7. The high-precision detection device for dendrobium phenol according to claim 1, characterized in that: The sealing cover is provided with a liquid injection connection port (37), and the liquid injection connection port (37) is connected to a liquid injection pipeline (38).

8. A high-precision detection method for dendrobium phenol, characterized in that: The steps include: Step 1: Wash and cut the dendrobium into small pieces, put the dendrobium into a container and heat it at 50 degrees for 30 minutes; Step 2: preparing an enzymatic hydrolysis solution, firstly putting the enzyme into low temperature water to dissolve it to form an enzymatic hydrolysis solution, adding the enzymatic hydrolysis solution into a container of dendrobium, enzymolyzing the dendrobium, the enzymatic hydrolysis temperature is 40 degrees, and the enzymatic hydrolysis time is 2 hours; Step 3: After enzymolysis, take out the dendrobium and crush it. During the crushing process, add an appropriate amount of clean water, the water level is 1 / 3 of the volume of the dendrobium, crush the dendrobium, and generate a crushed dendrobium solution; Step 4: Put the crushed dendrobium solution into the enzymatic solution again for secondary dissolution, the enzymatic temperature is 40 degrees, and the enzymatic time is 3 hours; Step 5: Filter out the dissolved solution and filter it through a filter with a 300-mesh filter; Step 6: Add a purifying agent to the dissolving solution to remove impurities, and then perform sedimentation. After 12 hours, extract the essence extract; Step seven, the extract is subjected to ultra-high performance liquid chromatography-electrochemical detection method to detect the content of dendrobium phenol.

9. A high-precision detection method for dendrobium phenol according to claim 8, characterized in that: The steps include: Step 1: Wash and cut the dendrobium into small pieces, put the dendrobium into the inner pot, and fill the upper space of the outer pot with water. The heating temperature is 50 degrees and the heating time is 30 minutes. Step 2: Prepare enzymatic hydrolysis solution. First, put the enzyme into low-temperature water to dissolve it to form an enzymatic hydrolysis solution. Add the enzymatic hydrolysis solution into the inner sleeve tank to enzymolyze the dendrobium. The enzymatic hydrolysis temperature is 40 degrees and the enzymatic hydrolysis time is 2 hours. Step 3: transport the enzymatic solution to the reflux box, and crush the dendrobium through the crushing mechanism and the inner sleeve tank. During the crushing process, add an appropriate amount of clean water, the water level is 1 / 3 of the volume of the dendrobium, and after the dendrobium is crushed, a crushed dendrobium solution is generated; Step 5: inject the enzymatic solution into the inner tank again to dissolve the dendrobium solution for a second time, the enzymatic hydrolysis temperature is 40 degrees, and the enzymatic hydrolysis time is 3 hours; Step 6: store the dissolved liquid in a reflux box, discharge the dendrobium residue, and clean the inner can; Step 7, transporting the dissolved liquid to the inner tank, and centrifuging and settling the liquid in the inner tank to obtain a supernatant essence extract; Step 8: Detect the content of dendrobium phenol in the essence extract by ultra-high performance liquid chromatography-electrochemical detection method.

Images