Kit and hematology analyzer
By designing a kit containing micropore tablets and electrodes, the problems of complex cleaning systems of traditional blood cell analyzers and high cost of POCT blood cell analyzers are solved, and the effect of reducing reagent costs and simplifying the analyzer structure is achieved.
Patent Information
- Application Number
- CN202311130066.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-08-31
- Publication Date
- 2025-02-28
AI Technical Summary
The cleaning system of traditional blood cell analyzers is complex in structure, with many components, and the cleaning process is time-consuming and reagent-consuming. While POCT blood cell analyzers simplify the instrument components, the kit design needs to accommodate multiple detection areas, resulting in an increase in product costs.
A kit is designed, including a box body, a micropore piece, a front cell electrode and a rear cell electrode. The box body is arranged as an adjacent front cell and a rear cell. The micropore piece is located between the front cell and the rear cell. The front cell electrode is arranged corresponding to the through hole at the bottom of the second cell body. The rear cell electrode is located in the rear cell. The micropore piece is arranged close to the front cell electrode to reduce the amount of samples to be detected.
By reducing the amount of samples to be tested, reducing the cost of reagents, simplifying the structure of the blood cell analyzer, and improving the reliability and user experience of the kit.
Smart Images
Figure CN119534821A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of sample analysis, and in particular to a reagent kit and a blood cell analyzer. Background Art
[0002] The blood cell analyzer, also known as blood cell analyzer, blood cell meter, blood cell counter, etc., is one of the most widely used instruments in clinical testing in hospitals.
[0003] A large part of the components in traditional blood cell analyzers belong to the cleaning system, because before the next blood sample test, it is necessary to ensure that the traces of the previous blood sample are cleaned. The entire cleaning system is not only complex in structure and has many components, but also requires a large amount of reagents during the cleaning process, which takes a long time.
[0004] POCT (Point-of-care Testing) blood cell analyzers have greatly simplified instrument components compared to traditional blood cell analyzers. POCT blood cell analyzers can use independent test kits to load sample fluids and reagents, and completely remove the cleaning fluid path-related components in traditional blood analysis, greatly reducing product complexity and production costs.
[0005] In addition, the POCT blood cell analyzer can perform electrical impedance detection and colorimetric detection on the sample solution in the test kit. Due to the structure of the test kit, the sample solution in the test kit needs to be subjected to two detection items, and the reagent pool for loading the sample solution needs to include detection areas for the two detection items. Therefore, the reagent pool needs to be designed with a larger capacity, and the amount of sample solution loaded in the test kit needs to be greater, which increases the product cost. Summary of the invention
[0006] The present application provides a kit to solve the above technical problems, and the kit comprises:
[0007] The box body comprises a front pool and a rear pool which are arranged adjacent to each other, wherein the front pool comprises a first pool body and a second pool body which are arranged in sequence along the height direction of the front pool, and a first through hole is provided at the bottom of the second pool body;
[0008] A microporous sheet, located between the front pool and the rear pool;
[0009] A front cell electrode and a rear cell electrode, wherein one end of the rear cell electrode is located in the rear cell, and one end of the front cell electrode is arranged corresponding to the first through hole, so that the front cell electrode contacts the sample to be detected in the second cell body;
[0010] Wherein, the microporous sheet is arranged corresponding to the second cell body and is arranged close to the front cell electrode.
[0011] The test kit is placed in a blood cell analyzer, and the blood cell analyzer is electrically connected to the front cell electrode and the rear cell electrode to perform a first item detection on the first sample to be detected in the second cell body; the light beam generated by the optical detection component of the blood cell analyzer passes through the second cell body to perform a second item detection on the second sample to be detected in the second cell body;
[0012] Wherein, the direction of the central axis of the microporous sheet is perpendicular to the optical path direction of the light beam.
[0013] The area of the first sample to be detected in the fore cell at least partially overlaps with the area of the second sample to be detected in the fore cell.
[0014] Wherein, the front cell electrode abuts against the end surface of the first through hole.
[0015] Wherein, the cross-sectional area of the second pool body gradually decreases in the opposite direction of the height direction.
[0016] Wherein, the cross-sectional area of the second cell body is smaller than the cross-sectional area of the first cell body.
[0017] Wherein, the box body comprises a second through hole, the second through hole is arranged on the side wall of the second cell body, and the second cell body is connected with the rear cell through the second through hole;
[0018] The second through hole is arranged close to the front cell electrode, and the microporous sheet is arranged corresponding to the second through hole, so that the sample to be detected enters the back cell through the second through hole and the microporous sheet.
[0019] The reagent box includes a baffle plate, which is arranged in the back pool to divide the back pool into a waste liquid chamber and a negative pressure chamber.
[0020] The second cell body is connected to the waste liquid chamber through the second through hole, and the waste liquid chamber is used to place the sample to be detected that passes through the microporous sheet;
[0021] The baffle has at least one third through hole, and the waste liquid chamber is connected with the negative pressure chamber through the third through hole;
[0022] Wherein, the plurality of third through holes are arranged offset from the central axis of the baffle.
[0023] The kit further comprises a shell, the shell comprising a first penetration seat and a second penetration seat, one end of the front cell electrode is arranged in the first penetration seat, and one end of the rear cell electrode is arranged in the second penetration seat;
[0024] Wherein, the first penetration seat and the second penetration seat are arranged side by side along the height direction of the reagent box.
[0025] In order to solve the above technical problems, the present application also provides a blood cell analyzer, which is used to perform sample detection on the sample to be detected in the kit as described above, and the blood cell analyzer includes an electrode assembly and an optical detection assembly;
[0026] The electrode assembly is electrically connected to the front cell electrode and the back cell electrode of the test kit to perform a first item test on the sample to be tested in the test kit;
[0027] The optical detection component generates a light beam to pass through the reagent box to perform a second item detection on the sample to be detected in the reagent box.
[0028] Beneficial effects of the present application: Different from the prior art, the kit of the present application includes a box body, a microporous sheet, a front cell electrode and a rear cell electrode. The box body includes a front cell and a rear cell arranged adjacent to each other, the front cell includes a first cell body and a second cell body arranged in sequence along the height direction of the front cell, a first through hole is provided at the bottom of the second cell body, and one end of the front cell electrode is arranged corresponding to the first through hole so that the front cell electrode contacts the sample to be detected in the second cell body. The microporous sheet is located between the front cell and the rear cell, and one end of the rear cell electrode is located in the rear cell. Among them, the microporous sheet is arranged corresponding to the second cell body and is arranged close to the front cell electrode. The sample to be detected in the second cell body enters the rear cell through the microporous sheet and contacts the rear cell electrode. The sample to be detected must at least cover the upper hole edge of the microporous sheet to enter the rear cell. Since the microporous sheet is arranged close to the front cell electrode, the liquid level of the sample to be detected in the second cell body decreases as the microporous sheet approaches the front cell electrode, reducing the amount of the sample to be detected in the second cell body and reducing the reagent cost. BRIEF DESCRIPTION OF THE DRAWINGS
[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0030] in:
[0031] Figure 1 It is a schematic structural diagram of the first embodiment of the kit of the present application;
[0032] Figure 2 yes Figure 1 A schematic diagram of the cross-sectional structure of the test kit along the section line II';
[0033] Figure 3 yes Figure 2 Schematic diagram of the structure of the middle area A;
[0034] Figure 4It is a schematic structural diagram of the third embodiment of the kit of the present application.
[0035] Figure numbers: reagent box 1; box body 11; front pool 111; first pool body 1111; second pool body 1112; first through hole 1113; rear pool 112; waste liquid chamber 1121; negative pressure chamber 1122; second through hole 113; microporous sheet 12; front pool electrode 13; rear pool electrode 14; baffle 15; third through hole 151; shell 16; first penetration seat 161; first protrusion 1611; second penetration seat 162; second protrusion 1621; first direction X; second direction Y. DETAILED DESCRIPTION
[0036] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. It is to be understood that the specific embodiments described herein are only used to explain the present application, rather than to limit the present application. It should also be noted that, for ease of description, only some structures related to the present application are shown in the accompanying drawings, rather than all structures. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.
[0037] The terms "first", "second", etc. in this application are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally includes steps or units that are not listed, or optionally includes other steps or units inherent to these processes, methods, products or devices.
[0038] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.
[0039] See also Figure 1 , Figure 11 is a schematic diagram of the structure of the first embodiment of the test kit of the present application. The test kit 1 provided in the embodiment of the present application is applied to a blood cell analyzer, such as a POCT blood cell analyzer. The test kit 1 exists independently of the blood cell analyzer, and the test kit 1 includes multiple pools, such as a sample pool, a diluent pool, a reagent pool, a detection pool, and a waste liquid pool, etc. The sample pool is used to load the sample liquid; the dilution pool is used to load the diluent; the reagent pool is used to load the reagent corresponding to the test item, the detection pool is used to load the sample to be tested mixed with the sample liquid and the diluent or the reagent, and serves as a test site; the waste liquid pool is used to load the sample waste liquid after the test is completed.
[0040] Furthermore, a test kit 1 loaded with a sample liquid and a reagent or a diluent for detection is placed in a blood cell analyzer. The blood cell analyzer uses the reagent or diluent on the test kit 1 to detect and analyze the sample liquid to obtain a test result of the sample liquid. The detection process is completed on the test kit 1. No additional liquid loading (sample liquid, diluent, reagent or cleaning liquid, etc.) components are required inside the blood cell analyzer, thereby simplifying the structure of the blood cell analyzer.
[0041] For details, please refer to Figure 2 , Figure 2 yes Figure 1 The cross-sectional structure diagram of the reagent box along the section line II′. The reagent box 1 comprises a box body 11 , a microporous sheet 12 , a front cell electrode 13 and a rear cell electrode 14 .
[0042] Among them, the box body 11 includes a front pool 111 and a rear pool 112 arranged adjacent to each other. The front pool 111 can be loaded with samples to be detected, serving as a detection site for the samples to be detected, that is, the detection pool mentioned above. In one embodiment, the front pool 111 and the rear pool 112 can be an integrally formed structure. In another embodiment, the front pool 111 and the rear pool 112 can also be a detachable assembly structure. The front pool 111 includes a first pool body 1111 and a second pool body 1112 arranged in sequence along a first direction X, and a first through hole 1113 is provided at the bottom of the second pool body 1112. The first direction X can be the height direction of the front pool 111, which is also the height direction of the reagent box 1.
[0043] The microporous sheet 12 is disposed between the front cell 111 and the rear cell 112. One end of the front cell electrode 13 is disposed corresponding to the first through hole 1113 so that the front cell electrode 13 contacts the sample to be detected in the second cell body 1112. One end of the rear cell electrode 14 is located in the rear cell 112.
[0044] Among them, the microporous sheet 12 includes micropores that allow blood cells to pass through one by one, and the front pool electrode 13 and the back pool electrode 14 are located on both sides of the microporous sheet 12 and are located in the same constant current circuit to perform impedance detection on the sample to be detected in the second pool body 1112. When the blood cells pass through the micropores of the microporous sheet 12 one by one, enter the back pool 112 and contact the back pool electrode 14, since the blood cells in the sample to be detected have poor conductor characteristics relative to the diluent, the resistance between the front pool electrode 13 and the back pool electrode 14 on both sides of the microporous sheet 12 changes (the resistance increases, and the degree of resistance change is proportional to the cell volume), thereby generating an instantaneous voltage change at both ends of the electrode to form a pulse signal, and the amplitude of the voltage pulse signal is proportional to the cell volume.
[0045] The blood cell analyzer can count the blood cells (leukocytes, basophils, red blood cells, platelets, etc.) in the sample to be tested by recording the pulse signal generated in the constant current circuit and the amplitude of the pulse signal.
[0046] The microporous sheet 12 is disposed corresponding to the second cell body 1112 and is disposed close to the front cell electrode 13 .
[0047] When the blood cell analyzer performs impedance detection on the sample to be detected, the sample to be detected must be in contact with the front cell electrode 13 and the rear cell electrode 14. The premise for the sample to be detected to enter the rear cell 112 and contact the rear cell electrode 14 is that the liquid level of the sample to be detected is higher than the upper hole edge of the microporous sheet 12. Therefore, the microporous sheet 12 and the second cell body 1112 are correspondingly arranged to ensure that the liquid level of the sample to be detected in the second cell body 1112 can submerge the upper hole edge of the microporous sheet 12. Among them, the upper hole edge of the microporous sheet 12 is the side of the micropores on the microporous sheet 12 away from the front cell electrode 13.
[0048] The microporous sheet 12 is arranged close to the forecell electrode 13. Figure 2 , the microporous sheet 12 is arranged close to the forecell electrode 13, that is, the microporous sheet 12 is arranged close to the forecell electrode 13 along the first direction X. As can be seen from the forecell electrode 13, the liquid level of the sample to be detected must be higher than the upper hole edge of the microporous sheet 12, and the closer the microporous sheet 12 is arranged to the forecell electrode 13, the lower the height of the upper hole edge of the microporous sheet 12 in the first direction X, and the liquid level of the sample to be detected in the second cell body 1112 can be lowered, thereby reducing the amount of the sample to be detected in the second cell body 1112 and reducing the reagent cost.
[0049] Among them, it can be obtained from the above that the prerequisite for the first item detection of the sample to be detected is that the sample to be detected is in contact with the front cell electrode 13 and in contact with the rear cell electrode 14 through the microporous sheet 12. Therefore, it can be obtained that when the sample to be detected in the reagent box 1 is detected for the first item, the amount of the sample to be detected in the reagent box 1 is the sum of the amount V1 of the sample to be detected required for the first item detection (hereinafter referred to as the amount V1), the amount V2 of the sample to be detected in the second cell body 1112 that is not passed through the upper hole edge of the microporous sheet 12 on the basis of the amount V1 (hereinafter referred to as the amount V2), and the amount V3 of the sample to be detected that is not passed through the upper hole edge of the microporous sheet 12 and enters the rear cell 112 and contacts the rear cell electrode 14 (hereinafter referred to as the amount V3).
[0050] Among them, the sample to be tested with a quantity V1 and the sample to be tested with a quantity V2 are located in the second cell body 1112, and the sum of the quantity V1 and the quantity V2 can just cover the upper hole edge of the microporous sheet 12; the sample to be tested with a quantity V3 is located in the rear cell 112, filling the accommodating space between the microporous sheet 12 and the rear cell electrode 14.
[0051] When the microporous sheet 12 is placed close to the forecell electrode 13 , the amount V1 is fixed, but the amounts V2 and V3 decrease as the microporous sheet 12 approaches the forecell electrode 13 , thereby reducing the amount of the sample to be tested in the reagent kit 1 and reducing the reagent cost in the reagent kit 1 .
[0052] The closer the microporous sheet 12 is to the front cell electrode 13, the better. It is necessary to ensure that the amount of the sample to be detected in the second cell body 1112 is sufficient for impedance detection. Therefore, the user can use the calculation of the amount of the sample to be detected in the reagent kit 1 and the idea of reducing the amount of the sample to be detected in the reagent kit 1 to set the position of the microporous sheet 12 in the reagent kit 1, thereby improving the accuracy of the position of the microporous sheet 12 in the reagent kit 1 and improving the reliability of the reagent kit 1.
[0053] Optionally, the reagent kit 1 is placed in a blood cell analyzer, and the blood cell analyzer is electrically connected to the front cell electrode 13 and the rear cell electrode 14 to perform a first item detection on the first sample to be detected in the second cell body 1112. The light beam generated by the optical detection component of the blood cell analyzer passes through the second cell body 1112 to perform a second item detection on the second sample to be detected in the second cell body 1112. The first item detection may be impedance detection, and the second item detection may be optical detection.
[0054] Specifically, the first sample to be tested for the first item test and the second sample to be tested for the second item test can be the same type of sample to be tested. The first sample to be tested and the second sample to be tested described in this embodiment are named after the item tests performed, not the types of samples to be tested.
[0055] For example, in the second cell body 1112, the front cell electrode 13 and the rear cell electrode 14 perform a first item detection on the liquid to be detected near the bottom of the second cell body 1112, and the sample to be detected participating in the first item detection is called the first sample to be detected; and the light beam generated by the optical detection component of the blood cell analyzer passes through the sample to be detected in the second cell body 1112 near the connection between the second cell body 1112 and the first cell body 1111, and performs a second item detection on the sample to be detected, and the sample to be detected in the second item detection is called the second sample to be detected. The blood cell analyzer can perform the first item detection and the second item detection on the sample to be detected in the second cell body 1112, respectively, that is, the first sample to be detected and the second sample to be detected can exist in the second cell body 1112 at the same time, therefore, the first sample to be detected and the second sample to be detected can be the same sample to be detected, which is only named differently according to the different items of detection performed.
[0056] Among them, optical detection is that the blood cell analyzer generates a light beam, and the light beam passes through the sample to be tested. The hemoglobin in the sample to be tested will absorb part of the light beam, and then the sample to be tested can obtain the concentration of hemoglobin in the sample to be tested by receiving and analyzing the transmitted light passing through the sample to be tested.
[0057] In one embodiment, the direction of the central axis of the microporous plate 12 is perpendicular to the optical path direction of the light beam.
[0058] The direction of the central axis of the microporous sheet 12 may be the second direction Y, which is also the length direction of the reagent box 1, and the optical path direction of the light beam is perpendicular to the second direction Y. Specifically, the optical detection device of the blood cell analyzer may include a light emitting component and a receiving component, which are respectively located on both sides of the second cell body 1112, so that the light beam emitted by the light emitting component enters one side of the second cell body 1112, and is emitted from the other side opposite to the second cell body 1112 after passing through the sample to be detected, and the receiving component is used to receive the emitted light beam.
[0059] The light emitting element and the receiving element may be located on the same horizontal plane, so that the light beam emitted by the light emitting element reaches the receiving element through the shortest distance, that is, the light beam enters the second cell body 1112 in a direction perpendicular to the first direction X and the second direction Y, and the area of the sample to be detected that the light beam passes through is the smallest, and at this time, the amount of the second sample to be detected for the second item detection is the smallest. If the light emitting element and the receiving element are not located on the same horizontal plane, the light beam emitted by the light emitting element enters the second cell body 1112 at an angle of non-90° with the first direction X, and the area of the sample to be detected that the light beam passes through becomes larger, that is, the area of the second detection item becomes larger, and the amount of the second sample to be detected becomes more.
[0060] In the case where the reagent kit 1 is to perform the first item detection and the second item detection, the amount of the sample to be detected in the second cell body 1112 is at least the sum of the amount of the first sample to be detected and the amount of the second sample to be detected. Therefore, if the amount of the second sample to be detected increases, the amount of the sample to be detected in the second cell body 1112 will increase. Therefore, the direction of the light path of the light beam emitted by the optical detection component is perpendicular to the direction (second direction Y) where the central axis of the microporous sheet 12 is located, and is perpendicular to the first direction X, so that the amount of the second sample to be detected is minimized, thereby reducing the total amount of the sample to be detected in the second cell body 1112 for the first item detection and the second item detection, reducing the reagent cost in the reagent kit 1, and improving the user experience of the reagent kit 1.
[0061] Moreover, since the central axis of the microporous sheet 12 is perpendicular to the optical path of the light beam emitted by the optical detection component, and the front cell electrode 13 is arranged at the bottom of the front cell 111, and the rear cell electrode 14 is arranged in the rear cell 112, the setting positions of the front cell electrode 13 and the rear cell electrode 14 will not block the light beam, and the light beam emitted by the optical detection component can be as close to the bottom of the front cell 111 as possible to lower the liquid level of the sample to be detected in the front cell 111.
[0062] Furthermore, the area of the first sample to be detected in the fore cell 111 at least partially overlaps with the area of the second sample to be detected in the fore cell 111 .
[0063] In one embodiment, the area of the first sample to be detected in the fore cell 111 at least partially overlaps with the area of the second sample to be detected in the fore cell 111, indicating that when the blood cell analyzer performs the first item detection and the second item detection in the fore cell 111, part of the samples to be detected can be reused. From the foregoing, it can be seen that the amount of the sample to be detected in the fore cell 111 is at least the sum of the amount of the first sample to be detected and the amount of the second sample to be detected. In this embodiment, part of the samples to be detected can be reused, which reduces the amount of the sample to be detected in the fore cell 111, achieves the technical purpose of reducing the amount of samples, reagents or diluents in the reagent kit 1, reduces the reagent cost in the reagent kit 1, and improves the user experience of the reagent kit 1.
[0064] Optionally, see Figure 3 , Figure 3 yes Figure 2 Schematic diagram of the structure of the middle area A. One end of the front cell electrode 13 abuts against the end surface of the first through hole 1113 .
[0065] Specifically, Figure 3As shown, the side of the forecell electrode 13 contacts the sample to be detected in the second cell body 1112 through the first through hole 1113. The forecell electrode 13 is arranged on the side of the first through hole 1113 away from the second cell body 1112, and is not located at the bottom of the second cell body 1112 through the first through hole 1113. And by the side of the forecell electrode 13 abutting against the end face of the first through hole 1113, the forecell electrode 13 seals the first through hole 1113, so as to prevent the sample to be detected in the second cell body 1112 from passing through the first through hole 1113 and causing leakage of the reagent kit 1.
[0066] In one embodiment, if the forecell electrode 13 is disposed at the bottom of the second cell body 1112 through one end of the first through hole 1113, due to the position of the forecell electrode 13, the second item detection area rises, and the liquid level of the sample to be detected in the second cell body 1112 rises, thereby increasing the amount of the sample to be detected. Therefore, in the present application, the forecell electrode 13 only abuts against the end surface of the first through hole 1113, reducing the amount of the sample to be detected in the second cell body 1112.
[0067] Optionally, the cross-sectional area of the second cell body 1112 gradually decreases in the opposite direction of the height direction (the first direction X).
[0068] In one embodiment, taking the first item test of the sample to be tested by the blood cell analyzer as an example, the liquid level of the sample to be tested loaded in the second pool body 1112 must be at least higher than the upper hole edge of the microporous sheet 12, and if the cross-sectional area of the second pool body 1112 gradually decreases in the opposite direction of the first direction X, it means that the second pool body 1112 is a structure that is narrow at the bottom and wide at the top. For the same amount of sample to be tested, the liquid level in the structure of the container with a constant cross-section is lower than the liquid level in the structure that is narrow at the bottom and wide at the top. Therefore, this embodiment can achieve that the liquid level of the sample to be tested is above the upper hole edge of the microporous sheet 12 with a smaller amount of sample to be tested, further reducing the amount of sample to be tested in the second pool body 1112 and reducing the reagent cost of the reagent kit 1.
[0069] Optionally, the cross-sectional area of the second cell body 1112 is smaller than the cross-sectional area of the first cell body 1111 .
[0070] Among them, the device in the blood cell analyzer draws the sample from the pool loaded with the sample on the test kit 1 and puts it into the second pool body 1112, and then draws the reagent or diluent (selected according to the requirements of the project test) from the pool loaded with the test kit 1 and puts it into the second pool body 1112, and mixes the sample and the reagent or diluent in the second pool body 1112 to form a sample to be tested.
[0071] In the process of injecting samples, reagents or diluents into the second cell body 1112, or in the process of mixing the samples to be tested, bubbles may be generated on the liquid surface of the liquid in the second cell body 1112. The cross-sectional area of the first cell body 1111 is larger than the cross-sectional area of the second cell body 1112, thereby preventing the second cell body 1112 from generating too many bubbles, causing the bubbles to overflow the front cell 111, or the bubbles from bursting and causing the liquid to splash out of the front cell 111, thereby improving the safety of the blood cell analyzer in performing sample testing on the samples in the test kit 1 and enhancing the user experience of the test kit 1.
[0072] Optionally, the box body 11 includes a second through hole 113 .
[0073] The second through hole 113 is disposed on the side wall of the second cell body 1112, and the second cell body 1112 is connected to the rear cell 112 through the second through hole 113. Specifically, the second through hole 113 is disposed on the side wall where the second cell body 1112 is connected to the rear cell 112, and the sample to be detected in the second cell body 1112 can reach the rear cell 112 through the second through hole 113 and the microporous sheet 12.
[0074] In one embodiment, the second through hole 113 is arranged near the front cell electrode 13, and the microporous sheet 12 is arranged corresponding to the second through hole 113. For example, the microporous sheet 12 is arranged on the side of the second through hole 113 close to the back cell 112, so that the sample to be detected in the second cell body 1112 enters the back cell 112 through the second through hole 113 and the microporous sheet 12. The microporous sheet 12 is arranged corresponding to the second through hole 113, ensuring that the sample to be detected through the second through hole 113 must pass through the microporous sheet 12 to enter the back cell 112.
[0075] If the microporous sheet 12 and the second through hole 113 are not arranged correspondingly, for example, the microporous sheet 12 and the second through hole 113 are arranged misaligned, it cannot be guaranteed that the sample to be detected entering the back pool 112 through the second through hole 113 passes through the microporous sheet 12, and the sample to be detected that does not pass through the microporous sheet 12 will not cause the resistance value between the front pool electrode 13 and the back pool electrode 14 to change, and the blood cell analyzer cannot perform impedance detection on the sample to be detected in the reagent kit 1. Therefore, the microporous sheet 12 and the second through hole 113 are arranged correspondingly, which ensures the objective conditions for the blood cell analyzer to perform impedance detection on the sample to be detected, and improves the reliability of the blood cell analyzer in performing sample detection on the sample in the reagent kit 1.
[0076] Optionally, see Figure 4 , Figure 4 The reagent kit 1 includes a baffle 15 disposed in the rear pool 112 to divide the rear pool 112 into a waste liquid chamber 1121 and a negative pressure chamber 1122 .
[0077] Among them, the second cell body 1112 is connected to the waste liquid chamber 1121 through the second through hole 113, and the waste liquid chamber 1121 is used to place the sample to be detected that passes through the microporous sheet 12. Specifically, the sample to be detected in the second cell body 1112 enters the back cell 112 and contacts the back cell electrode 14 after passing through the second through hole 113 and the microporous sheet 12. After the sample to be detected entering the back cell 112 contacts the back cell electrode 14, the sample to be detected becomes a sample liquid that has completed the test, also known as sample waste liquid. At this time, the sample waste liquid further enters the waste liquid chamber 1121, and the waste liquid chamber 1121 stores the sample waste liquid. There is no need to set up an additional container for loading the sample waste liquid. After the sample to be detected completes the first item test, it immediately enters the waste liquid chamber 1121 from the liquid flow direction for storage. The test kit 1 has a simple structure, removes the sample waste liquid processing step of the blood cell analyzer for the first item test of the sample to be detected, simplifies the sample detection step, and improves the efficiency of the blood cell analyzer in performing sample detection on the sample to be detected in the test kit 1.
[0078] The baffle 15 has at least one third through hole 151, and the waste liquid chamber 1121 is connected to the negative pressure chamber 1122 through the third through hole 151. Specifically, the negative pressure chamber 1122 provides negative pressure to the waste liquid chamber 1121 through the third through hole 151, so that the sample to be detected in the second cell body 1112 enters the waste liquid chamber 1121 through the second through hole 113 and the microporous sheet 12 under the action of the negative pressure and contacts the rear cell electrode 14, and finally the sample waste liquid is stored in the waste liquid chamber 1121.
[0079] In one embodiment, the plurality of third through holes 151 are disposed offset from the central axis of the baffle 15 .
[0080] For details, please refer to Figure 4 From the perspective of the present invention, the plurality of third through holes 151 in the reagent box 1 are arranged to the left or right of the central axis of the baffle 15. For example, the plurality of third through holes 151 are arranged to the left of the central axis of the baffle 15, and when the reagent box 1 is tipped to the right, the height of the third through holes 151 is higher than the liquid level of the sample waste liquid in the waste liquid chamber 1121, thereby preventing the sample waste liquid in the waste liquid chamber 1121 from flowing back into the negative pressure chamber 1122 and leaking out of the negative pressure chamber 1122 when the reagent box 1 is tipped, thereby improving the safety of the reagent box 1.
[0081] In one embodiment, after the test of the sample to be tested in the reagent kit 1 is completed, the user takes the reagent kit 1 out of the blood cell analyzer and places it on the operating table. At this time, the reagent kit 1 placed on the operating table may be tipped over due to the user's operation and touch. If the third through hole 151 is set at the central axis of the baffle 15 at this time, the liquid level of the sample waste liquid in the waste liquid chamber 1121 will be higher than the height of the third through hole 151. The sample waste liquid enters the negative pressure chamber 1122 from the waste liquid chamber 1121 through the third through hole 151 and leaks out of the negative pressure chamber 1122, causing leakage of the reagent kit 1.
[0082] Therefore, the embodiment of the present application proposes that a plurality of third through holes 151 are arranged offset from the central axis of the baffle 15. It is understandable that the third through holes 151 can be arranged according to the user's operating habits or the placement of the device. For example, when operating, the user is accustomed to placing the reagent kit 1 unloaded from the hematology analyzer on the left hand side, that is, the possibility that the reagent kit 1 is accidentally touched by the user and tilted to the left is relatively high, then the third through hole 151 can be arranged to the right of the central axis of the baffle 15; or, the position on the operating table for placing the unloaded reagent kit 1 is located on the right hand side of the user, and the possibility that the reagent kit 1 is accidentally touched by the user and tilted to the right is relatively high, then the third through hole 151 can be arranged to the left of the central axis of the baffle 15. The present application does not limit the setting position of the third through hole 151, and the user can set it according to their own needs.
[0083] Optionally, see Figure 3 The reagent kit 1 also includes a shell 16 .
[0084] The housing 16 includes a first penetration seat 161 and a second penetration seat 162. One end of the front cell electrode 13 is disposed in the first penetration seat 161, and one end of the rear cell electrode 14 is disposed in the second penetration seat 162. The first penetration seat 161 and the second penetration seat 162 are disposed in parallel along the first direction X.
[0085] The first penetration seat 161 and the second penetration seat 162 are arranged in parallel along the first direction X, that is, the first penetration seat 161 and the second penetration seat 162 are arranged up and down in the first direction X. In one embodiment, the second penetration seat 162 is arranged close to the first penetration seat 161, so that the rear cell electrode 14 is arranged close to the front cell electrode 13, so as to reduce the amount of samples to be detected in the first item detection and reduce the amount of samples to be detected in the second cell body 1112.
[0086] The first penetration seat 161 is provided with at least one first protrusion 1611 , and the first protrusion 1611 abuts against the fore cell electrode 13 .
[0087] In one embodiment, since one end of the forecell electrode 13 contacts the sample to be detected in the second cell body 1112 through the first through hole 1113, the sample to be detected in the second cell body 1112 may flow through one end of the forecell electrode 13 to the other end of the forecell electrode 13, that is, leak out of the reagent box 1, causing leakage of the reagent box 1. If there is a first protrusion 1611 in the first penetration seat 161 that abuts against the forecell electrode 13, the first protrusion 1611 will block the flow of the sample to be detected flowing from one end of the forecell electrode 13 to the other end, avoiding leakage of the reagent box 1 and improving the safety of the reagent box 1. Further, the forecell electrode 13 may include a groove corresponding to the first protrusion 1611, and then the first protrusion 1611 cooperates with the groove to further fix the forecell electrode 13 on the first penetration seat 161, further improving the safety of the reagent box 1.
[0088] Optionally, the second penetration seat 162 is provided with at least one second protrusion 1621 , and the second protrusion 1621 abuts against the rear cell electrode 14 .
[0089] In one embodiment, since one end of the back cell electrode 14 is located in the back cell 112 and contacts the sample to be detected that enters the back cell 112 through the microporous sheet 12, the sample to be detected in the back cell 112 may flow through one end of the back cell electrode 14 to the other end of the back cell electrode 14. And leak out of the reagent box 1. A second protrusion 1621 that abuts against the back cell electrode 14 is provided in the second penetration seat 162, and the second protrusion 1621 can block the flow of the sample to be detected that flows from one end of the back cell electrode 14 to the other end, thereby improving the safety of the reagent box 1. Further, the back cell electrode 14 may include a groove corresponding to the second protrusion 1621, and the second protrusion 1621 cooperates with the groove to fix the back cell electrode 14 on the second penetration seat 162.
[0090] In summary, the reagent kit 1 proposed in the embodiment of the present application includes a front pool 111 and a rear pool 112 arranged adjacent to each other, and the front pool 111 and the rear pool 112 are connected through the second through hole 113 and the microporous sheet 12, one end of the front pool electrode 13 is arranged at the bottom of the front pool 111, one end of the rear pool electrode 14 is arranged in the rear pool, and the microporous sheet 12 is arranged close to the front pool electrode 13, so as to reduce the amount of the sample to be detected in the second pool body 1112. And the cross-sectional area of the second pool body 1112 gradually decreases in the opposite direction of the height direction, and the amount of the sample to be detected is reduced when the liquid level of the sample to be detected in the second pool body 1112 is increased.
[0091] Moreover, the area where the blood cell analyzer performs the first item test on the samples in the test kit 1 and the area where the second item test is performed at least partially overlap, and some of the samples in the second pool body 1112 can be reused when performing the first item test and the second item test, which can further reduce the amount of samples to be tested and reduce the reagent cost of the test kit 1.
[0092] The reagent kit 1 also includes a baffle 15 that divides the rear pool 112 into a waste liquid chamber 1121 and a negative pressure chamber 1122. The baffle 15 has a third through hole 151 that is set away from the central axis of the baffle 15. The off-center axis setting of the third through hole 151 can prevent the reagent kit 1 from tipping over and leaking. In addition, a first protrusion 1611 prevents the sample to be tested from leaking due to the front pool electrode 13; a second protrusion 1621 prevents the sample to be tested from leaking due to the rear pool electrode 14, thereby improving the safety of the reagent kit 1 and enhancing the user experience of the reagent kit 1.
[0093] The present application also provides a blood cell analyzer (not shown), wherein the blood cell analyzer may be a POCT blood cell analyzer, etc. The blood cell analyzer is used to perform sample detection on the sample to be detected in the kit 1 .
[0094] Among them, the blood cell analyzer includes an electrode assembly and an optical detection assembly. The electrode assembly is electrically connected to the front cell electrode 13 and the rear cell electrode 14 of the reagent kit 1 to perform a first item detection on the sample to be detected in the reagent kit 1; the optical detection assembly generates a light beam to pass through the sample to be detected in the reagent kit 1, and performs a second item detection on the sample to be detected.
[0095] In one embodiment, the blood cell analyzer may first perform the first item test on the sample to be tested in the reagent kit 1, and then perform the second item test; or, first perform the second item test on the sample to be tested, and then perform the first item test; or, the blood cell analyzer simultaneously performs the first item test and the second item test on the reagent to be tested in the reagent kit 1. In the case where the blood cell analyzer performs the first item test on the sample to be tested and a loop with a large current is formed in the sample to be tested, the current loop may affect the light beam entering the sample to be tested, and in this case, it is preferred that the blood cell analyzer performs the first item test and the second item test in time.
[0096] When the blood cell analyzer performs the first item test and the second item test in different time periods, it is preferred to perform the second item test first and then the first item test, so as to reduce the amount of the sample to be tested in the second pool body 1112. If the first item test is performed first, part of the sample liquid to be tested in the second pool body 1112 will enter the back pool 112, and the amount of the sample to be tested in the second pool body 1112 will be reduced. However, since the second item test is to be performed, it is necessary to ensure that after the first item test is performed, the amount of the sample to be tested required for the second item test still exists in the second pool body 1112, that is, the initial amount of the sample to be tested in the second pool body 1112 is to be increased.
[0097] If the second item is tested first, and if the amount of samples to be tested required for the second item is more than that required for the first item, it is only necessary to ensure that the amount of samples to be tested in the second pool body 1112 is the amount of samples to be tested for the second item. After the second item is tested, the samples to be tested in the second pool body 1112 are directly tested for the first item.
[0098] Alternatively, if the amount of the sample to be tested required for the first item detection is greater than the amount of the sample to be tested required for the second item detection, it is only necessary to ensure that the amount of the sample to be tested in the second pool body 1112 is the amount of the sample to be tested for the first item detection. After the second item detection is completed, the amount of the sample to be tested in the second pool body 1112 is still the amount of the sample to be tested required for the first item detection, and the blood cell analyzer directly performs the first item detection on the sample to be tested in the second pool body 1112. By performing the second item detection first and then the first item detection, the amount of the sample to be tested in the kit 1 is further reduced, and the reagent cost of the kit 1 is reduced.
[0099] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.
Claims
1. A kit, characterized in that: include: The box body comprises a front pool and a rear pool which are adjacently arranged, wherein the front pool comprises a first pool body and a second pool body which are sequentially arranged along the height direction of the front pool, and a first through hole is provided at the bottom of the second pool body; A microporous sheet, located between the front pool and the rear pool; A front cell electrode and a rear cell electrode, wherein one end of the rear cell electrode is located in the rear cell, and one end of the front cell electrode is arranged corresponding to the first through hole, so that the front cell electrode contacts the sample to be detected in the second cell body; Wherein, the microporous sheet is arranged corresponding to the second cell body and is arranged close to the front cell electrode.
2. The kit according to claim 1, characterized in that The test kit is placed in a blood cell analyzer, and the blood cell analyzer is electrically connected to the front cell electrode and the rear cell electrode to perform a first item test on the first sample to be tested in the second cell body; the light beam generated by the optical detection component of the blood cell analyzer passes through the second cell body to perform a second item test on the second sample to be tested in the second cell body; Wherein, the direction of the central axis of the microporous sheet is perpendicular to the optical path direction of the light beam.
3. The kit according to claim 2, characterized in that The area of the first sample to be detected in the fore cell at least partially overlaps with the area of the second sample to be detected in the fore cell.
4. The kit according to claim 1, characterized in that The fore cell electrode abuts against an end surface of the first through hole.
5. The kit according to claim 1, characterized in that The cross-sectional area of the second pool body gradually decreases in the opposite direction of the height direction.
6. The kit according to any one of claims 1 to 5, characterized in that The cross-sectional area of the second cell body is smaller than the cross-sectional area of the first cell body.
7. The kit according to any one of claims 1 to 5, characterized in that The box body comprises a second through hole, the second through hole is arranged on the side wall of the second cell body, and the second cell body is connected with the rear cell through the second through hole; The second through hole is arranged close to the front cell electrode, and the microporous sheet is arranged corresponding to the second through hole, so that the sample to be detected enters the back cell through the second through hole and the microporous sheet.
8. The kit according to claim 7, characterized in that The reagent box includes a baffle plate, which is arranged in the back pool to divide the back pool into a waste liquid chamber and a negative pressure chamber. The second cell body is connected to the waste liquid chamber through the second through hole, and the waste liquid chamber is used to place the sample to be detected that passes through the microporous sheet; The baffle has at least one third through hole, and the waste liquid chamber is connected with the negative pressure chamber through the third through hole; Wherein, the plurality of third through holes are arranged offset from the central axis of the baffle.
9. The kit according to claim 1, characterized in that The kit also includes a shell, the shell including a first penetration seat and a second penetration seat, one end of the front cell electrode is arranged in the first penetration seat, and one end of the rear cell electrode is arranged in the second penetration seat; Wherein, the first penetration seat and the second penetration seat are arranged side by side along the height direction of the reagent box.
10. A blood cell analyzer, characterized in that: Applicable to sample detection of a sample to be detected in a kit as claimed in any one of claims 1 to 9, the blood cell analyzer comprises an electrode assembly and an optical detection assembly; The electrode assembly is electrically connected to the front cell electrode and the back cell electrode of the test kit to perform a first item test on the sample to be tested in the test kit; The optical detection component generates a light beam to pass through the reagent box to perform a second item detection on the sample to be detected in the reagent box.