Streaming detection device and streaming detection method

Abstract

The invention discloses a flow type detection device and a flow type detection method.The flow type detection device comprises a flow chamber assembly, the flow chamber assembly comprises a sheath flow pool and a sample needle, and the flow chamber assembly is used for enabling sample flow flowing out of a first opening of the sample needle to form axial laminar flow movement located at the axis of the sheath flow pool in sheath flow of the sheath flow pool, carrying out flow detection; the power module is connected with the flow chamber assembly; and the control module is used for controlling the power module to provide pressure for the sample flow in the sample needle, suck back the sample flow in the sample needle and enable the sheath flow to enter the sample needle through the first opening after the flow type detection of the flowing sample flow is completed by the flow chamber assembly. Based on the above mode, the accuracy of stream detection can be effectively improved.

Description

Technical Field

[0001] The present application relates to the field of detection technology, and in particular to a flow detection device and a flow detection method. Background Art

[0002] In the prior art, the flow chamber assembly includes a sheath flow pool and a sample needle. The sample flow provided by the sample needle and the sheath flow provided by the sheath flow pool form a laminar flow, thereby enabling the sample flow to flow to the detection area in the flow chamber assembly in the laminar flow for flow detection.

[0003] The defect of the prior art is that, in the flow chamber assembly, when the sheath flow of the sheath flow pool is unstable due to cleaning after the current flow detection operation is completed, the sample flow in the sample needle is likely to flow out and contaminate the inner wall of the sheath flow pool, thereby easily causing negative interference to the subsequent flow detection operation and reducing the accuracy of the flow detection. Summary of the invention

[0004] The main technical problem solved by this application is how to improve the accuracy of flow cytometry detection.

[0005] In order to solve the above technical problems, the first technical solution adopted in the present application is: a flow detection device, comprising: a flow chamber assembly, the flow chamber assembly comprising a sheath flow pool and a sample needle, the flow chamber assembly being used to make the sample flow flowing out of the first opening of the sample needle form an axial laminar flow motion located at the axis of the sheath flow pool in the sheath flow of the sheath flow pool to perform flow detection; a power module, the power module is connected to the flow chamber assembly; and a control module, the control module is used to: after the flow chamber assembly completes the flow detection of the outflowing sample flow, control the power module to provide pressure to the sample flow in the sample needle, suck back the sample flow in the sample needle, and make the sheath flow enter the sample needle through the first opening.

[0006] Wherein, the power module includes: a first suction and exhalation module, the first suction and exhalation module is connected to the second opening of the sample needle; the control module is used to: after the flow chamber component completes the flow detection of the outflowing sample flow, control the first suction and exhalation module to provide negative pressure, suck back the sample flow in the sample needle and make the sheath flow enter the sample needle through the first opening; and / or, the power module includes: a third suction and exhalation module, the third suction and exhalation module is connected to an opening of the sheath flow pool; the control module is used to: after the flow chamber component completes the flow detection of the outflowing sample flow, control the third suction and exhalation module to provide positive pressure to push the sheath flow into the sample needle through the first opening and suck back the sample flow in the sample needle.

[0007] Among them, the flow detection device also includes: a liquid aspiration needle, the first opening of the liquid aspiration needle is connected to the second opening of the sample needle; a waste liquid pool; the power module includes: a waste liquid pump, the waste liquid pump is used to connect the second opening of the liquid aspiration needle, and the waste liquid pump is connected to the waste liquid pool; the flow chamber component has a sample needle switch valve, and the sample needle switch valve is used to conduct or disconnect the connection between the sample needle and the sheath flow pool; the control module is used to: after the sample flow in the sample needle is sucked back and discharged from the sample needle, control the sample needle switch valve to close; control the waste liquid pump to provide negative pressure so that the corresponding sample flow passes through the liquid aspiration needle and the waste liquid pump into the waste liquid pool.

[0008] Among them, the flow detection device also includes: a cleaning module, the cleaning module is used to clean the outer wall of the pipette needle, and an opening of the cleaning module is connected to the waste liquid pump; a first three-way joint, the first opening of the first three-way joint is connected to the pipette needle, the second opening of the first three-way joint is connected to the sample needle, and the third opening of the first three-way joint is connected to the first suction and exhalation module; a first three-way valve, the first branch port of the first three-way valve is connected to another opening of the cleaning module, the second branch port of the first three-way valve is connected to the third opening of the first three-way joint, and the common port of the first three-way valve is connected to the first suction and exhalation module; controlling the waste liquid pump to provide negative pressure so that the corresponding sample flow passes through the pipette needle and the waste liquid pump into the waste liquid pool, including: when controlling the second opening of the pipette needle to move to the cleaning inner cavity of the cleaning module, controlling the waste liquid pump to provide negative pressure so that the corresponding sample flow passes through the pipette needle, the cleaning module and the waste liquid pump into the waste liquid pool.

[0009] Among them, the power module also includes: a second suction and exhalation module; the flow detection device also includes: a second three-way joint, the first opening of the second three-way joint is connected to the second opening of the sample needle, and the second opening of the second three-way joint is connected to the first three-way joint; a second suction and exhalation module, the second suction and exhalation module is connected to the third opening of the second three-way joint; controlling the waste liquid pump to provide negative pressure so that the corresponding sample flow passes through the suction needle and the waste liquid pump into the waste liquid pool, including: controlling the second suction and exhalation module to provide positive pressure, and / or controlling the first three-way valve to connect its second branch port and common port and controlling the first suction and exhalation module to provide positive pressure; controlling the waste liquid pump to provide negative pressure so that the corresponding sample flow passes through the suction needle, the cleaning module and the waste liquid pump into the waste liquid pool.

[0010] Among them, the flow detection device also includes a pipette needle; a first switch valve, one opening of the first switch valve is connected to the first opening of the pipette needle, and the second opening of the pipette needle is used to aspirate and exhale samples; a first three-way connector, the first opening of the first three-way connector is connected to the other opening of the first switch valve, the second opening of the first three-way connector is connected to the second opening of the sample needle, and the third opening of the first three-way connector is connected to the first aspiration module; the control module is used to: control the second opening of the pipette needle to move to the sample aspiration position; control the first switch valve to open; control the first aspiration module to provide negative pressure, and transport the sample flow to the pipeline between the first three-way connector and the pipette needle through the pipette needle.

[0011] Among them, the flow detection device also includes: a second three-way connector, the first opening of the second three-way connector is connected to the second opening of the sample needle, and the second opening of the second three-way connector is connected to the first opening of the first switch valve; a second switch valve, one opening of the second switch valve is connected to the third opening of the second three-way connector; a second suction and exhalation module, the second suction and exhalation module is connected to the other opening of the second switch valve; the control module is used to: control the first switch valve to open, and control the second switch valve to open; control the second suction and exhalation module to provide negative pressure to transport the sample flow, so that part of the sample flow is located between the first three-way connector and the second three-way connector, and part of the sample flow is located between the second three-way connector and the second suction and exhalation module; control the second switch valve to close; control the power module to provide negative pressure to the sample flow in the sample needle through the second opening of the sample needle, suck back the sample flow in the sample needle and allow the sheath flow to enter the sample needle through the first opening, including: after controlling the second switch valve to close, control the power module to provide negative pressure to the sample flow in the sample needle through the second opening of the sample needle, suck back the sample flow in the sample needle and allow the sheath flow to enter the sample needle through the first opening.

[0012] Among them, the flow detection device also includes: a third three-way connector, the first opening of the third three-way connector is connected to the third opening of the first three-way connector, and the second opening of the third three-way connector is connected to the first suction and exhalation module; a third switch valve, an opening of the third switch valve is connected to the third opening of the third three-way connector; a fourth three-way connector, the first opening of the fourth three-way connector is connected to the third opening of the third three-way connector, and the second opening of the fourth three-way connector is connected to an opening of the sheath flow pool; a third suction and exhalation module, the third suction and exhalation module is connected to the third opening of the fourth three-way connector; the control module is used to: control the first switch valve and the second switch valve to close, and control the third switch valve to open; control the first suction and exhalation module and the third suction and exhalation module to provide positive pressure to transport the sample flow into the sample needle, and push the sheath flow in the sheath flow pool, so that the sample flow flowing out of the first opening forms an axial laminar flow motion located at the axis of the sheath flow pool in the sheath flow of the sheath flow pool.

[0013] Among them, controlling the first switch valve and the second switch valve to close and controlling the third switch valve to open includes: controlling the first switch valve and the second switch valve to close, and controlling the third switch valve to open within a first preset time period; the control module is used to: control the third switch valve to close after the first preset time period.

[0014] In order to solve the above-mentioned technical problems, the second technical solution adopted in the present application is: a flow detection method, applied to the flow detection device as claimed in any one of claims 1 to 9; the flow detection method comprises: after the flow chamber assembly completes the flow detection of the outflowing sample flow, controlling the power module to provide negative pressure to the sample flow in the sample needle through the second opening of the sample needle, sucking back the sample flow in the sample needle and allowing the sheath flow to enter the sample needle through the first opening.

[0015] The beneficial effect of the present application lies in that, different from the prior art, in the technical solution of the present application, after the flow chamber assembly completes the corresponding flow detection operation, pressure is first applied to the sample needle so that the sample flow in the sheath flow pool is refluxed through the sample needle, and the sheath flow in the sheath flow pool is sucked into the sample needle, so that there is no sample flow between the sample needle and the sheath flow pool in the flow chamber assembly, thereby avoiding the situation in which the sample flow contaminates the inner wall of the sheath flow pool due to the instability of the subsequent sheath liquid during cleaning of the flow chamber assembly, thereby reducing the possibility of the current sample flow causing negative interference to the subsequent flow detection operation, and improving the accuracy of the flow detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1 It is a structural schematic diagram of an embodiment of the flow detection device of the present application.

[0018] Wherein: flow chamber assembly 101, sheath flow pool 1011, sample needle 1012, first suction and exhalation module 102, suction needle 103, waste liquid pool 104, waste liquid pump 105, first switch valve 106, first three-way joint 107, second three-way joint 108, second switch valve 109, second suction and exhalation module 110, third three-way joint 111, third switch valve 112, fourth three-way joint 113, third suction and exhalation module 114, cleaning module 115, first three-way valve 116, sheath liquid storage module 117, second three-way valve 118, third three-way valve 119, fourth three-way valve 120, fourth switch valve 121. DETAILED DESCRIPTION

[0019] The present application is further described in detail below in conjunction with the accompanying drawings and examples. It is particularly noted that the following examples are only used to illustrate the present application, but are not intended to limit the scope of the present application. Similarly, the following examples are only some embodiments of the present application rather than all embodiments, and all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present application.

[0020] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0021] In the description of this application, it is necessary to explain that, unless otherwise clearly specified and limited, the terms "install", "set", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or connected through an intermediate medium. For ordinary technicians in this field, the above-mentioned specific meanings belonging to this application can be connected according to specific circumstances.

[0022] It should be noted that the three-way connector, three-way valve, and other connectors or valves mentioned in this application can be specific devices or components with corresponding functions. A single component can be composed of multiple parts. For example:

[0023] A three-way valve can be made up of two two-way valves, or modified from a four-way valve; a three-way connector can be made up of two two-way connectors, or modified from a four-way connector. As can be seen from the above, the three-way connector or three-way valve of the present application can be simply replaced by a corresponding component with the function of a three-way connector or three-way valve, which is not limited here, and such simple replacement is included in the scope of patent protection of the present application.

[0024] This application proposes a flow detection device, see Figure 1 , Figure 1 is a schematic diagram of the structure of an embodiment of the flow detection device of the present application, such as Figure 1 As shown, the flow detection device includes a flow chamber assembly 101, a power module and a control module.

[0025] The flow chamber assembly 101 includes a sheath flow pool 1011 and a sample needle 1012. The first opening of the sample needle 1012 is located in the sheath flow pool 1011. The flow chamber assembly 101 is used to make the sample flow flowing out of the first opening of the sample needle 1012 form an axial laminar flow motion located at the axis center of the sheath flow pool 1011 in the sheath flow of the sheath flow pool 1011 to perform flow detection.

[0026] The power module is connected to the flow chamber assembly 101. The sample needle 1012 is used to provide power to the sample flow in the sample needle.

[0027] The power module may be connected to the sample needle 1012, and the power module is used to provide power to the sample flow in the sample needle so that the sample flow flows accordingly.

[0028] The flow chamber component may specifically refer to an optical flow chamber component, and the optical flow chamber component may specifically be a flow chamber component used for flow detection in a flow fluorescence immunoassay analyzer.

[0029] The measurement method of the flow fluorescence immunoassay analyzer is to apply the principle of fluid focusing, using sheath fluid to wrap the sample liquid to be tested, so that the sample liquid forms an axial laminar flow motion located at the axis of the sheath flow pool. The objects to be tested in the sample liquid pass through the detection area in turn, and the optical measurement module is used to detect the objects to be tested and obtain the test results.

[0030] During the measurement stage, the sheath liquid pressure and the sample flow pressure maintain a relatively stable state to ensure the sample flow state is stable, and the measurement results will be more accurate. After the measurement is completed, the sample liquid in the pipeline needs to be cleaned. At this time, the sheath liquid pressure and the sample liquid pressure will change and cannot maintain a stable state. At this time, the sample flow is prone to jitter, causing the sample liquid to hit the wall, that is, the sample liquid will contact the inner wall of the sheath flow cell, causing the inner wall of the sheath flow cell to be contaminated, the sheath flow cell carryover rate to increase, and the sheath flow cell cleaning efficiency to decrease, affecting the accuracy of the test results of subsequent continuous detection.

[0031] The power module can specifically be a suction and discharge module in a flow detection device, or a waste liquid pump, or other hydraulic or pneumatic power devices that can provide positive or negative pressure to the sample needle 1012. The specific configuration can be determined based on actual needs and is not limited here.

[0032] The control module is used to:

[0033] After the flow chamber assembly 101 completes the flow detection of the sample flow, the power module is controlled to provide pressure to the sample flow in the sample needle 1012 , to suck back the sample flow in the sample needle 1012 , and to allow the sheath flow to enter the sample needle 1012 through the first opening.

[0034] Specifically, after the flow chamber assembly 101 completes the flow detection of the sample flow, the power module can be controlled to provide negative pressure to the sample flow in the sample needle 1012 through the second opening of the sample needle 1012, so as to suck back the sample flow in the sample needle 1012 and allow the sheath flow to enter the sample needle 1012 through the first opening.

[0035] After the flow chamber assembly 101 completes the flow detection of the sample flow in the current flow detection operation, it is usually necessary to perform a cleaning operation on the flow chamber assembly 101. After the flow chamber assembly 101 completes the current flow detection operation, before the flow chamber assembly 101 is cleaned, a negative pressure can be provided to the sample needle 1012 through the power module to suck back the sample liquid in the sample needle 1012, and further, when there is no sample flow in the sheath flow pool, the sheath liquid in the sheath flow pool 1011 also flows into the sample needle, so that the sample liquid in the sample needle cannot directly contact the sheath flow pool, thereby reducing the possibility of the sample liquid in the sheath flow pool contaminating the inner wall of the sheath flow pool when the sheath flow is unstable during the subsequent cleaning operation, thereby improving the reliability and accuracy of the subsequent flow detection operation.

[0036] Different from the prior art, in the technical solution of the present application, after the flow chamber assembly completes the corresponding flow detection operation, pressure is first applied to the sample needle so that the sample flow in the sample can flow back through the sample needle, or the sheath flow in the sheath flow pool can be sucked into the sample needle, so that there is no sample flow between the sample needle in the flow chamber assembly and the sheath flow pool, thereby avoiding the situation where the sample flow contaminates the inner wall of the sheath flow pool due to the instability of the subsequent sheath liquid during cleaning of the flow chamber assembly, thereby reducing the possibility of the current sample flow causing negative interference to the subsequent flow detection operation and improving the accuracy of the flow detection.

[0037] In one embodiment, if Figure 1 As shown, the power module may include a first suction and discharge module 102 .

[0038] The first aspiration module 102 is connected to the second opening of the sample needle 1012 .

[0039] The control module is used to:

[0040] After the flow chamber assembly completes the flow detection of the outflowing sample flow, the first aspiration module 102 is controlled to provide negative pressure to suck back the sample flow in the sample needle and allow the sheath flow to enter the sample needle 1012 through the first opening.

[0041] and / or,

[0042] like Figure 1 As shown, the power module includes a third suction and exhalation module 114 , and the third suction and exhalation module 114 is connected to an opening of the sheath flow pool 1011 .

[0043] The control module is used to:

[0044] After the flow chamber assembly 101 completes the flow detection of the outflowing sample flow, the third aspiration module 114 is controlled to provide positive pressure to push the sheath flow into the sample needle 1012 through the first opening and to aspirate the sample flow in the sample needle 1012 .

[0045] The control module is used to:

[0046] After the flow chamber assembly 101 completes the flow detection of the outflowing sample flow, the third aspiration module 114 is controlled to provide positive pressure to push the sheath flow into the sample needle 1012 through the first opening and to aspirate the sample flow in the sample needle 1012 .

[0047] Specifically, in one example, the first suction module 102 can be a syringe with a second opening connected to the sample needle 1012, and the syringe can generally be used to push the liquid in the corresponding pipeline so that the sample liquid enters the flow chamber assembly 101 in a preset flow manner for flow detection.

[0048] After completing the flow detection operation, by controlling the first suction and exhalation module 102 to provide negative pressure to suck back the sample flow in the flow chamber assembly 101, all the sample flow and part of the sheath flow in the sample needle can flow into the sample needle 1012 in sequence, so that the sample liquid in the sample needle cannot directly contact the sheath flow pool, thereby reducing the possibility of the sample liquid in the sheath flow pool contaminating the inner wall of the sheath flow pool when the sheath flow is unstable during the subsequent cleaning operation, thereby improving the reliability and accuracy of the subsequent flow detection operation.

[0049] In another example, after completing the flow detection operation, by controlling the third suction and discharge module 114 to provide positive pressure to push the sample flow in the flow chamber assembly 101, at least part of the sheath flow in the sheath flow pool 1011 can flow into the sample needle 1012, so that the sample liquid in the sample needle cannot directly contact the sheath flow pool, thereby reducing the possibility of the sample liquid in the sheath flow pool contaminating the inner wall of the sheath flow pool when the sheath flow is unstable due to the subsequent cleaning operation, thereby improving the reliability and accuracy of the subsequent flow detection operation.

[0050] Optionally, the flow detection device may further include a liquid aspiration needle 103 and a waste liquid pool 104 .

[0051] The first opening of the pipetting needle 103 is connected to the second opening of the sample needle 1012 .

[0052] The power module may include a waste pump 105 .

[0053] The waste liquid pump 105 is used to connect to the second opening of the aspiration needle 103 , and the waste liquid pump 105 is connected to the waste liquid tank 104 .

[0054] The flow chamber assembly has a sample needle switch valve, which is used to open or close the connection between the sample needle 1012 and the sheath flow cell 1011 .

[0055] The control module is used to:

[0056] After the sample flow in the sample needle 1012 is sucked back and discharged from the sample needle 1012 , the sample needle switch valve is controlled to close.

[0057] The waste liquid pump 105 is controlled to provide negative pressure so that the corresponding sample flow passes through the aspiration needle 103 and the waste liquid pump 105 and enters the waste liquid tank 104 .

[0058] Specifically, in practice, the waste liquid pump 105 can be connected to an opening of the cleaning module 115 set on the pipette needle 103, and the other opening of the cleaning module 115 is connected to the corresponding suction and exhalation module. The outer wall of the pipette needle can be cleaned by the cleaning liquid (such as sheath liquid) provided by the corresponding suction and exhalation module, and then the cleaning waste liquid is transported to the waste liquid tank 104 by the waste liquid pump 105. The second opening of the pipette needle 103 can be moved to the cleaning space of the cleaning module 115, and the suction force provided by the waste liquid pump 105 makes the sample flow flowing out from the second opening of the pipette needle 103 be sucked into the waste liquid tank 104.

[0059] like Figure 1 As shown, positive pressure can be provided to the sheath flow pool 1011 through the third suction and discharge module 114 to provide positive pressure to the sample flow in the sample needle 1012 through the first opening of the sample needle 1012, so that the sample flow in the sample needle 1012 flows out from the second opening of the sample needle 1012 and the sheath flow enters the sample needle 1012 through the first opening. At this time, the sample needle switch valve can be closed to block the connection between the sample needle 1012 and the sheath flow pool 1011, and then the negative pressure provided by the waste liquid pump 105 is used to push the sample flow flowing out from the second opening of the sample needle 1012 to the aspiration needle 103 for discharge. Based on the above method, different power sources can be used to efficiently perform the action of sucking back the sample flow at different positions, thereby improving the sample flow sucking efficiency, thereby improving the overall efficiency of sample detection, and thus improving the reliability of the flow detection device.

[0060] Furthermore, the power module also includes:

[0061] The first suction and exhalation module 102.

[0062] The flow detection device also includes:

[0063] The cleaning module 115 is used to clean the outer wall of the liquid aspiration needle 103 . An opening of the cleaning module 115 is connected to the waste liquid pump 105 .

[0064] The first three-way connector 107 has a first opening connected to the liquid aspiration needle 103 , a second opening connected to the sample needle, and a third opening connected to the first aspiration module 102 .

[0065] The first three-way valve 116 , the first branch port of the first three-way valve 116 is connected to another opening of the cleaning module 115 , the second branch port of the first three-way valve 116 is connected to the third opening of the first three-way connector 107 , and the common port of the first three-way valve 116 is connected to the first suction and exhalation module 102 .

[0066] Controlling the waste liquid pump 105 to provide negative pressure so that the corresponding sample flows through the aspiration needle 103 and the waste liquid pump 105 into the waste liquid pool 104, including:

[0067] When the second opening of the pipetting needle 103 is controlled to move to the cleaning inner cavity of the cleaning module 115 , the waste liquid pump 105 is controlled to provide negative pressure so that the corresponding sample flow passes through the pipetting needle 103 , the cleaning module 115 and the waste liquid pump 105 and enters the waste liquid pool 104 .

[0068] Specifically, Figure 1 As shown, the washable module 115 can specifically be a cleaning swab, and the cleaning inner cavity can be an inner cavity in the cleaning swab for cleaning the outer wall of the aspiration needle 103 for allowing the cleaning liquid to flow through, so that the sample liquid flowing out of the aspiration needle 103 can be sucked into the waste liquid pool 104 by the waste liquid pump 105, so as to complete the waste liquid collection, avoid the need to set up an additional waste liquid discharge channel between the waste liquid pool 104 and the aspiration needle 103, and instead use the connection between the aspiration needle 103 and the cleaning module 115, thereby improving the utilization efficiency of each component in the flow detection device and reducing the cost of the flow detection device.

[0069] Furthermore, the power module also includes:

[0070] The second suction and discharge module 110 .

[0071] The flow detection device also includes:

[0072] The second three-way connector 108 has a first opening connected to the second opening of the sample needle, and a second opening of the second three-way connector 108 connected to the first three-way connector 107 .

[0073] The second suction and exhalation module 110 is connected to the third opening of the second three-way connector 108 .

[0074] Controlling the waste liquid pump 105 to provide negative pressure so that the corresponding sample flows through the aspiration needle 103 and the waste liquid pump 105 into the waste liquid pool 104, including:

[0075] The second suction and exhalation module 110 is controlled to provide positive pressure, and / or the first three-way valve 116 is controlled to connect its second branch port and the common port and the first suction and exhalation module 102 is controlled to provide positive pressure.

[0076] The waste liquid pump 105 is controlled to provide negative pressure so that the corresponding sample flow passes through the aspiration needle 103 , the cleaning module 115 and the waste liquid pump 105 and enters the waste liquid tank 104 .

[0077] Specifically, after the sample needle switch valve is closed, the waste liquid pump 105 can be controlled to provide negative pressure, and at least one of the second suction and exhalation module 110 and the first suction and exhalation module 102 can be controlled to provide corresponding thrust to the sample flow between the sample needle 1012 and the aspiration needle 103, so that the sample flow flows toward the aspiration needle 103. The more suction and exhalation modules are used, the higher the thrust efficiency is, which can improve the overall efficiency of sample detection, thereby improving the reliability of the flow detection device.

[0078] Alternatively, if Figure 1 As shown, the power module includes the above-mentioned first suction and discharge module 102, and the flow detection device also includes the above-mentioned liquid aspiration needle 103.

[0079] The flow detection device further includes a first switch valve 106 and a first three-way connector 107 .

[0080] An opening of the first switch valve 106 is connected to a first opening of the aspiration needle 103 , and a second opening of the aspiration needle 103 is used for aspirating the sample.

[0081] The first opening of the first three-way joint 107 is connected to the other opening of the first switch valve 106 , the second opening of the first three-way joint 107 is connected to the aspiration needle 103 , and the third opening of the first three-way joint 107 is connected to the first aspiration module 102 .

[0082] The control module is used to:

[0083] The second opening of the liquid aspiration needle 103 is controlled to move to the sample aspiration position.

[0084] The first switch valve 106 is controlled to open.

[0085] The first suction and discharge module 102 is controlled to provide negative pressure, and the sample flow is delivered to the pipeline between the first three-way connector 107 and the suction needle 103 through the suction needle 103 .

[0086] Specifically, when performing flow detection operations, it is usually necessary to first obtain samples. The second opening of the aspiration needle 103 can be moved to the sample aspiration position. The sample aspiration position can specifically refer to a test tube position, or other sample aspiration positions containing samples, which is not limited here.

[0087] Then open the first switch valve 106, and provide negative pressure through the first suction module 102 to suck the sample from the sample suction position into the pipeline between the first three-way connector 107 and the aspiration needle 103, so as to prevent the sample from entering the pipeline between the first three-way connector 107 and the first suction module 102, thereby reducing the possibility of contamination of the first suction module 102 by the sample flow and improving the accuracy of flow detection.

[0088] Furthermore, if Figure 1 As shown, the flow detection device also includes a second three-way connector 108 , a second switch valve 109 and a second suction and discharge module 110 .

[0089] The first opening of the second three-way connector 108 is connected to the second opening of the sample needle 1012 , and the second opening of the second three-way connector 108 is connected to the first opening of the first switch valve 106 .

[0090] One opening of the second switch valve 109 is connected to the third opening of the second three-way connector 108 .

[0091] The second suction and discharge module 110 is connected to the other opening of the second switch valve 109 .

[0092] The control module is used to:

[0093] The first on-off valve 106 is controlled to be open, and the second on-off valve 109 is controlled to be open.

[0094] The second suction and exhalation module 110 is controlled to provide negative pressure to transport the sample flow so that part of the sample flow is located between the first three-way connector 107 and the second three-way connector 108 , and part of the sample flow is located between the second three-way connector 108 and the second suction and exhalation module 110 .

[0095] The second switch valve 109 is controlled to be closed.

[0096] The power module is controlled to provide negative pressure to the sample flow in the sample needle 1012 through the second opening of the sample needle 1012, so as to suck back the sample flow in the sample needle 1012 and allow the sheath flow to enter the sample needle 1012 through the first opening, including:

[0097] After controlling the second switch valve 109 to close, the control power module provides negative pressure to the sample flow in the sample needle 1012 through the second opening of the sample needle 1012, sucks back the sample flow in the sample needle 1012 and allows the sheath flow to enter the sample needle 1012 through the first opening.

[0098] Specifically, after the aspiration needle 103 delivers the sample flow to the pipeline between the first three-way connector 107 and the aspiration needle 103, the second aspiration and exhalation module 110 can be controlled to deliver the sample flow along the corresponding pipeline toward the second aspiration and exhalation module 110 through the second switch valve 109, the second three-way connector 108, the first three-way connector 107 and the first switch valve 106, so that the front end part of the sample flow is located between the second three-way connector 108 and the second aspiration and exhalation module 110, and at this time, the second switch valve 109 is closed to isolate the front end part of the sample flow from other parts of the sample flow. Since the front end part of the sample flow is usually in contact with and diluted by the sheath fluid in the pipeline, the above method can ensure that all samples entering the flow chamber assembly 101 are as undiluted as possible, thereby improving the accuracy of flow detection.

[0099] In addition, by controlling the second switch valve 109 to close and then executing the above-mentioned step of aspirating the sample flow in the sample needle 1012, it can be ensured that the sample flow that flows back due to aspiration can accurately flow in the direction of the aspiration needle 103 for discharge, and will not mistakenly flow to the second aspiration and discharge module 110 and eventually cause contamination, thereby improving the reliability of flow detection.

[0100] Furthermore, if Figure 1 As shown, the flow detection device also includes a third three-way connector 111, a third switch valve 112, a fourth three-way connector 113, and a third suction and discharge module 114.

[0101] The first opening of the third three-way joint 111 is connected to the third opening of the first three-way joint 107 , and the second opening of the third three-way joint 111 is connected to the first suction and discharge module 102 .

[0102] An opening of the third switch valve 112 is connected to the third opening of the third three-way connector 111 .

[0103] The first opening of the fourth three-way joint 113 is connected to the third opening of the third three-way joint 111 , and the second opening of the fourth three-way joint 113 is connected to an opening of the sheath flow cell 1011 .

[0104] The third suction and discharge module 114 is connected to the third opening of the fourth three-way connector 113 .

[0105] The control module is used to:

[0106] The first on-off valve 106 and the second on-off valve 109 are controlled to be closed, and the third on-off valve 112 is controlled to be opened.

[0107] The first suction and exhalation module 102 and the third suction and exhalation module 114 are controlled to provide positive pressure to deliver the sample flow into the sample needle 1012 and push the sheath flow in the sheath flow pool 1011, so that the sample flow flowing out of the first opening of the sample needle 1012 forms an axial laminar flow motion located at the axis center of the sheath flow pool 1011 in the sheath flow of the sheath flow pool 1011.

[0108] Specifically, based on the above method, when both the first suction and exhalation module 102 and the third suction and exhalation module 114 are controlled to provide positive pressure, a part of the thrust of the third suction and exhalation module 114 is used to promote the sheath liquid in the sheath flow pool 1011 to flow, and another part of the thrust of the third suction and exhalation module 114 is used together with the entire thrust of the first suction and exhalation module 102 to promote the flow of the sample flow in the flow chamber assembly 101, thereby improving the stability of the constructed laminar flow motion and improving the sample flow delivery efficiency.

[0109] Furthermore, if Figure 1 As shown, controlling the first switch valve 106 and the second switch valve 109 to close, and controlling the third switch valve 112 to open may specifically include:

[0110] The first switch valve 106 and the second switch valve 109 are controlled to be closed, and the third switch valve 112 is controlled to be opened within a first preset time period.

[0111] The control module is used to:

[0112] After the first preset time period, the third switch valve 112 is controlled to close.

[0113] Specifically, after the laminar flow motion is stabilized, that is, after the sample flows stably in the sheath flow in the flow chamber assembly 101, the third switch valve 112 can be closed so that all the thrust of the third suction and exhalation module 114 is used to flow the sheath liquid in the sheath flow pool 1011, further ensuring the durability of the stable laminar flow motion formed and improving the accuracy of flow detection.

[0114] In one embodiment, if Figure 1 As shown, when the flow detection device further includes the aspiration needle 103, the first switch valve 106, the first three-way connector 107, the second three-way connector 108, the second switch valve 109 and the second aspiration module 110 described in the above embodiment, the control module is used to:

[0115] The first on-off valve 106 and the second on-off valve 109 are controlled to be open.

[0116] The second suction and discharge module 110 is controlled to provide positive pressure to transport the liquid in the corresponding pipeline for cleaning.

[0117] Specifically, the stored sheath fluid can be pushed along the pipeline between the aspiration needle 103, the first switch valve 106, the first three-way connector 107, the second three-way connector 108, the second switch valve 109 and the second aspiration module 110 through the second aspiration module 110, so as to achieve the cleaning of the corresponding pipeline and the aspiration needle 103. Based on the above method, all the pipelines and the aspiration needle 103 through which the sample flow passes outside the flow chamber assembly 101 can be cleaned at one time, thereby improving the cleaning efficiency.

[0118] In one embodiment, if Figure 1 As shown, when the flow detection device further includes the liquid aspiration needle 103 , the waste liquid pool 104 and the waste liquid pump 105 described in the previous embodiment, the flow detection device further includes a cleaning module 115 and a first three-way valve 116 .

[0119] The cleaning module 115 is used to clean the outer wall of the pipette needle 103 , and an opening of the cleaning module 115 is connected to the waste liquid pump 105 .

[0120] The first branch port of the first three-way valve 116 is connected to another opening of the cleaning module 115 , the second branch port of the first three-way valve 116 is connected to the third opening of the first three-way connector 107 , and the common port of the first three-way valve 116 is connected to the first suction and discharge module 102 .

[0121] The control module is used to:

[0122] The first three-way valve 116 is controlled to connect its first branch port and its common port.

[0123] The first suction and discharge module 102 is controlled to provide positive pressure to transport the liquid in the corresponding pipeline for cleaning.

[0124] Specifically, the cleaning module 115 can be a cleaning swab for cleaning the outer wall of the pipette needle 103. The cleaning swab can slide up and down relative to the pipette needle 103, that is, the cleaning swab can slide up and down relative to the pipette needle 103, and the pipette needle 103 can also slide up and down relative to the cleaning swab. It is not limited here. The outer wall of the pipette needle 103 is cleaned through the sheath liquid flow space therein, and the waste liquid after cleaning can be pumped away into the waste liquid pool 104 through the waste liquid pump 105.

[0125] The stored sheath liquid can flow through the first suction and discharge module 102 along the pipeline between the first three-way valve 116, the cleaning module 115 and the waste liquid pool 104, so as to clean the outer wall of the aspiration needle 103 and improve the cleaning reliability.

[0126] In one embodiment, if Figure 1As shown, the flow detection device also includes the third switch valve 112, the fourth three-way connector 113, the third suction and discharge module 114, the waste liquid pool 104 and the waste liquid pump 105 described in the above embodiments.

[0127] The waste liquid reservoir 104 is connected to another opening of the sheath flow reservoir 1011 .

[0128] Flow cytometry devices are used for:

[0129] The third switch valve 112 is controlled to be closed.

[0130] The third suction and discharge module 114 is controlled to provide positive pressure to transport the liquid in the corresponding pipeline through the sheath flow pool 1011 to the waste liquid pool 104 .

[0131] Specifically, based on the above method, the flow chamber assembly 101 can be cleaned through the third suction module 114, and the cleaning waste liquid can be transported to the waste liquid pool 104, so as to achieve efficient cleaning of the flow chamber assembly 101 without passing through other pipelines, thereby improving the cleaning efficiency.

[0132] In one embodiment, if Figure 1 As shown, based on the structure described in any of the above embodiments, the flow detection device may further include a sheath fluid storage module 117 , a second three-way valve 118 , a third three-way valve 119 , a fourth three-way valve 120 and a fourth switch valve 121 .

[0133] The common port of the second three-way valve 118 is connected to the second suction and discharge module 110 , and the first branch port of the second three-way valve 118 is connected to the sheath liquid storage module 117 .

[0134] The common port of the third three-way valve 119 is connected to the third suction and discharge module 114 , the first branch port of the third three-way valve 119 is connected to the sheath liquid storage module 117 , and the second branch port of the third three-way valve 119 is connected to the third opening of the fourth three-way connector 113 .

[0135] The common port of the fourth three-way valve 120 is connected to the second branch port of the second three-way valve 118 , the first branch port of the fourth three-way valve 120 is connected to the other opening of the second switch valve 109 , and the second branch port of the fourth three-way valve 120 is connected to the first suction and discharge module 102 .

[0136] One opening of the fourth switch valve 121 is connected to another opening of the sheath flow cell 1011 , and the other opening of the fourth switch valve 121 is connected to the waste liquid cell 104 .

[0137] Specifically, the second suction and exhalation module 110 and the third suction and exhalation module 114 can be connected to the sheath liquid storage module 117 through corresponding three-way valves to replenish the sheath liquid therein, and connected to corresponding pipelines through corresponding three-way valves to suction and exhale the sheath liquid.

[0138] The fourth three-way valve 120 can be switched so that the second suction and exhalation module 110 is connected to the second switch valve 109 or the first suction and exhalation module 102 .

[0139] The fourth switch valve 121 can be used to open or close the pipeline between the flow chamber assembly 101 and the waste liquid pool 104. For example, it can be used to open the pipeline when cleaning the flow chamber assembly.

[0140] Based on the above method, the flow detection device can have stronger flexibility and improve the applicability of the flow detection device.

[0141] The present application also proposes a flow detection method, which can be applied to the flow detection device described in any of the above embodiments.

[0142] Flow cytometry methods include:

[0143] After the flow chamber assembly 101 completes the flow detection of the sample flow, the control power module provides negative pressure to the sample flow through the sample needle 1012 to suck back the sample flow and allow the sheath flow to enter the sample needle 1012 through the first opening.

[0144] Specifically, the streaming detection method may be the execution logic of the control module described above, which will not be described in detail here.

[0145] Different from the prior art, in the technical solution of the present application, after the flow chamber assembly completes the corresponding flow detection operation, pressure is first applied to the sample needle so that the sample flow in the sample can flow back through the sample needle, or the sheath flow in the sheath flow pool can be sucked into the sample needle, so that there is no sample flow between the sample needle in the flow chamber assembly and the sheath flow pool, thereby avoiding the situation where the sample flow contaminates the inner wall of the sheath flow pool due to the instability of the subsequent sheath liquid during cleaning of the flow chamber assembly, thereby reducing the possibility of the current sample flow causing negative interference to the subsequent flow detection operation and improving the accuracy of the flow detection.

[0146] In the description of the present application, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradicting each other.

[0147] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0148] Any process or method description in a flowchart or otherwise described herein may be understood to represent a module, segment or portion of code that includes one or more executable instructions for implementing the steps of a specific logical function or process, and the scope of the preferred embodiments of the present application includes alternative implementations in which functions may not be performed in the order shown or discussed, including performing functions in a substantially simultaneous manner or in the reverse order depending on the functions involved, which should be understood by technicians in the technical field to which the embodiments of the present application belong.

[0149] The logic and / or steps represented in the flowchart or otherwise described herein, for example, can be considered as an ordered list of executable instructions for implementing the logical functions, and can be embodied in any computer-readable medium for use by an instruction execution system, device or apparatus (which can be a personal computer, server, network device or other system that can fetch instructions from the instruction execution system, device or apparatus and execute the instructions), or in combination with these instruction execution systems, devices or apparatuses. For the purpose of this specification, "computer-readable medium" can be any device that can contain, store, communicate, propagate or transmit a program for use by an instruction execution system, device or apparatus or in combination with these instruction execution systems, devices or apparatuses. More specific examples of computer-readable media (a non-exhaustive list) include the following: an electrical connection with one or more wires (electronic device), a portable computer disk box (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable and programmable read-only memory (EPROM or flash memory), a fiber optic device, and a portable compact disk read-only memory (CDROM). In addition, the computer-readable medium may even be paper or other suitable medium on which the program is printed, since the program may be obtained electronically, for example, by optically scanning the paper or other medium and then editing, interpreting or processing in other suitable ways if necessary, and then stored in a computer memory.

[0150] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A flow detection device, characterized in that: include: A flow chamber assembly, the flow chamber assembly comprising a sheath flow cell and a sample needle, the flow chamber assembly being used to make the sample flow flowing out of the first opening of the sample needle form an axial laminar flow motion located at the axis of the sheath flow cell in the sheath flow of the sheath flow cell, so as to perform flow detection; a power module, the power module being connected to the flow chamber assembly; A control module, wherein the control module is used to: After the flow chamber assembly completes the flow detection of the outflowing sample flow, the power module is controlled to provide pressure to the sample flow in the sample needle, to suck back the sample flow in the sample needle, and to allow the sheath flow to enter the sample needle through the first opening.

2. The flow detection device according to claim 1, characterized in that: The power module comprises: A first suction and exhalation module, wherein the first suction and exhalation module is connected to the second opening of the sample needle; The control module is used for: After the flow chamber assembly completes the flow detection of the outflowing sample flow, controlling the first suction and discharge module to provide negative pressure, sucking back the sample flow in the sample needle and allowing the sheath flow to enter the sample needle through the first opening; and / or, The power module comprises: a third suction and exhalation module, the third suction and exhalation module being connected to an opening of the sheath flow pool; The control module is used for: After the flow chamber assembly completes the flow detection of the outflowing sample flow, the third aspiration module is controlled to provide positive pressure to push the sheath flow into the sample needle through the first opening and to aspirate the sample flow in the sample needle.

3. The flow detection device according to claim 2, characterized in that: The flow detection device also includes: A pipette needle, wherein a first opening of the pipette needle is connected to a second opening of the sample needle; Wastewater tank; The power module comprises: A waste liquid pump, the waste liquid pump is used to connect to the second opening of the aspiration needle, and the waste liquid pump is connected to the waste liquid tank; The flow chamber assembly has a sample needle switch valve, and the sample needle switch valve is used to open or close the connection between the sample needle and the sheath flow cell; The control module is used for: After the sample flow in the sample needle is sucked back and discharged from the sample needle, controlling the sample needle switch valve to close; The waste liquid pump is controlled to provide negative pressure so that the corresponding sample flow passes through the aspiration needle and the waste liquid pump and enters the waste liquid tank.

4. The flow detection device according to claim 3, characterized in that: The flow detection device also includes: A cleaning module, the cleaning module is used to clean the outer wall of the liquid-pipetting needle, an opening of the cleaning module is connected to the waste liquid pump; A first three-way connector, wherein a first opening of the first three-way connector is connected to the aspiration needle, a second opening of the first three-way connector is connected to the sample needle, and a third opening of the first three-way connector is connected to the first aspiration module; A first three-way valve, wherein a first branch port of the first three-way valve is connected to another opening of the cleaning module, a second branch port of the first three-way valve is connected to a third opening of the first three-way connector, and a common port of the first three-way valve is connected to the first suction and discharge module; The step of controlling the waste liquid pump to provide negative pressure so that the corresponding sample flows through the aspiration needle and the waste liquid pump into the waste liquid pool comprises: When controlling the second opening of the aspiration needle to move to the cleaning inner cavity of the cleaning module, controlling the waste liquid pump to provide negative pressure, so that the corresponding sample flow passes through the aspiration needle, the cleaning module and the waste liquid pump into the waste liquid pool.

5. The flow detection device according to claim 4, characterized in that: The power module also includes: The second suction and discharge module; The flow detection device also includes: A second three-way connector, wherein a first opening of the second three-way connector is connected to a second opening of the sample needle, and a second opening of the second three-way connector is connected to the first three-way connector; a second suction and exhalation module, the second suction and exhalation module being connected to the third opening of the second three-way connector; The step of controlling the waste liquid pump to provide negative pressure so that the corresponding sample flows through the aspiration needle and the waste liquid pump into the waste liquid pool comprises: Control the second suction and exhalation module to provide positive pressure, and / or control the first three-way valve to connect its second branch port and common port and control the first suction and exhalation module to provide positive pressure; The waste liquid pump is controlled to provide negative pressure so that the corresponding sample flow passes through the aspiration needle, the cleaning module and the waste liquid pump and enters the waste liquid pool.

6. The flow detection device according to any one of claims 2 to 5, characterized in that: The flow detection device also includes The aspiration needle; A first switch valve, wherein an opening of the first switch valve is connected to a first opening of the aspiration needle, and a second opening of the aspiration needle is used for aspirating a sample; A first three-way connector, wherein a first opening of the first three-way connector is connected to another opening of the first switch valve, a second opening of the first three-way connector is connected to a second opening of the sample needle, and a third opening of the first three-way connector is connected to the first suction module; The control module is used for: Controlling the second opening of the aspiration needle to move to a sample aspiration position; Controlling the first switch valve to open; The first suction and exhalation module is controlled to provide negative pressure, and the sample flow is delivered to the pipeline between the first three-way connector and the suction needle through the suction needle.

7. The flow detection device according to claim 6, characterized in that: The flow detection device also includes: a second three-way connector, wherein a first opening of the second three-way connector is connected to a second opening of the sample needle, and a second opening of the second three-way connector is connected to a first opening of the first switch valve; a second switch valve, wherein an opening of the second switch valve is connected to a third opening of the second three-way connector; a second suction and exhalation module, the second suction and exhalation module being connected to another opening of the second switch valve; The control module is used for: Controlling the first switch valve to open, and controlling the second switch valve to open; Controlling the second suction and exhalation module to provide negative pressure to deliver the sample flow, so that part of the sample flow is located between the first three-way connector and the second three-way connector, and part of the sample flow is located between the second three-way connector and the second suction and exhalation module; Controlling the second switch valve to close; The controlling the power module to provide negative pressure to the sample flow in the sample needle through the second opening of the sample needle, to suck back the sample flow in the sample needle and to allow the sheath flow to enter the sample needle through the first opening, comprises: After controlling the second switch valve to close, controlling the power module to provide negative pressure to the sample flow in the sample needle through the second opening of the sample needle, sucking back the sample flow in the sample needle and allowing the sheath flow to enter the sample needle through the first opening.

8. The flow detection device according to claim 7, characterized in that: The flow detection device also includes: A third three-way connector, wherein a first opening of the third three-way connector is connected to a third opening of the first three-way connector, and a second opening of the third three-way connector is connected to the first suction and discharge module; a third switch valve, wherein an opening of the third switch valve is connected to a third opening of the third three-way connector; a fourth three-way connector, wherein a first opening of the fourth three-way connector is connected to a third opening of the third three-way connector, and a second opening of the fourth three-way connector is connected to an opening of the sheath flow cell; A third suction and exhalation module, the third suction and exhalation module is connected to the third opening of the fourth three-way connector; The control module is used for: Controlling the first switch valve and the second switch valve to close, and controlling the third switch valve to open; The first suction and exhalation module and the third suction and exhalation module are controlled to provide positive pressure to deliver the sample flow into the sample needle and push the sheath flow in the sheath flow pool, so that the sample flow flowing out of the first opening forms an axial laminar flow motion located at the axis center of the sheath flow pool in the sheath flow of the sheath flow pool.

9. The flow detection device according to claim 8, characterized in that: The controlling the first switch valve and the second switch valve to close and the controlling the third switch valve to open comprises: Controlling the first switch valve and the second switch valve to close, and controlling the third switch valve to open within a first preset time period; The control module is used for: After the first preset time period, the third switch valve is controlled to close.

10. A flow cytometry method, characterized in that: Applicable to the flow detection device according to any one of claims 1 to 9; The flow detection method comprises: After the flow chamber assembly completes the flow detection of the outflowing sample flow, the power module is controlled to provide negative pressure to the sample flow in the sample needle through the second opening of the sample needle, so as to suck back the sample flow in the sample needle and allow the sheath flow to enter the sample needle through the first opening.

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