Non-contact temperature control method and system for small animal experiment

By using non-contact laser heating and infrared temperature measurement technology in the experimental animal temperature control system, the problems of inaccurate mechanoreceptor activation and temperature control caused by contact heating are solved, and more accurate and local heating control is achieved, and the temperature control accuracy reaches within 0.1℃.

CN119536405APending Publication Date: 2025-02-28INST OF ACUPUNCTURE & MOXIBUSTION CHINA ACADEMY OF CHINESE MEDICAL SCI
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Patent Information

Application Number
CN202411689021.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-25
Publication Date
2025-02-28

AI Technical Summary

Technical Problem

The existing experimental animal temperature control system adopts contact heating, which leads to a large range of activation and heating of mechanoreceptors, and the accuracy of temperature control is not high.

Method used

The non-contact laser heating method is adopted to heat the target area through the laser heating unit, and the infrared temperature measurement module is used to monitor the temperature of the target skin in real time. The output power and focus distance of the laser heating unit are adjusted through the control system to achieve closed-loop control.

Benefits of technology

The disadvantages of contact heating are avoided, and the activation and heating range of mechanoreceptors are reduced, making heating more precise and local. At the same time, by real-time monitoring of the temperature of the target skin, more accurate temperature control is achieved, and the temperature control accuracy error is within 0.1℃.

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Abstract

The invention discloses a non-contact temperature control method and system for a small animal experiment, and belongs to the technical field of experimental animal temperature control. The temperature control method comprises the following steps: S01, fixing an experimental small animal; s02, setting a preset temperature, and carrying out non-contact heating on the target area by adopting a laser heating unit; s03, an infrared temperature measurement unit is adopted to accurately detect the temperature of the target area, the temperature detected in real time is fed back to a control system unit, and the control system unit compares temperature differences; the control system regulates and controls the output power of the laser heating unit; and S04, when the real-time detection temperature of the infrared temperature measurement unit is equal to the preset temperature, non-contact temperature control is completed. According to the invention, non-contact heating of the laser heating unit is adopted; real-time monitoring temperature feedback of the infrared temperature measuring unit is received through the control system unit, accurate heating is performed step by step in a heating mode, accurate control over non-contact temperature control is achieved, and the temperature control accuracy error is within 0.1 DEG C.
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Description

Technical Field

[0001] The invention belongs to the technical field of experimental animal temperature control, and in particular relates to a non-contact temperature control method and system for small animal experiments. Background Art

[0002] The somatosensory system is one of the most complex sensory systems in the body, and temperature sensation, as an important component of somatosensory sensation, has attracted much attention from researchers at home and abroad. The 2021 Nobel Prize in Physiology or Medicine was awarded to David Julius, a neuroscientist who made outstanding contributions to the study of thermal pain receptors. The premise of understanding the transmission of temperature information is to be able to accurately control the amplitude and range of temperature stimulation in animal experiments. However, there is currently a lack of experimental equipment in this area, which cannot meet the experimental requirements.

[0003] The commonly used heating method at present is to give cold or hot stimulation to the skin through contact methods such as water bath heating. However, this heating method will cause the activation of some mechanical receptors because the skin is in direct contact with water or other heating media, and the heating range is relatively large. In addition, the temperature control feedback of common experimental temperature control stimulation devices measures the temperature at the output end. Therefore, the current experimental animal temperature control system has the disadvantages of contact heating and the problem of low accuracy of temperature control feedback measurement at the output end. Summary of the invention

[0004] The embodiment of the present invention provides a non-contact temperature control method and system for small animal experiments. The invention aims to solve the problem that the existing temperature control system adopts contact heating and the temperature cannot be accurately detected in real time. The temperature control device provided by the present invention adopts a laser heating method to avoid the disadvantages of contact heating; and the infrared temperature measurement module equipped with the device measures the real-time temperature of the target skin, so that the temperature control is more accurate.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A non-contact temperature control method for small animal experiments, specifically comprising the following steps:

[0007] S01. Fix the experimental animal so that the distance between the target area to be heated and the laser heating unit is between 1mm and 20mm;

[0008] S02. Setting a preset temperature, using a laser heating unit to heat the target area, and achieving non-contact heating of the target area by adjusting the output power and focusing distance of the laser heating unit;

[0009] S03. Use an infrared temperature measurement unit to accurately detect the temperature of the target area, and feed back the real-time detected temperature to the control system unit. The control system unit compares the difference between the real-time detected temperature and the preset temperature; the control system unit adjusts the output power temperature of the laser heating unit;

[0010] S04. When the real-time detected temperature of the infrared temperature measuring unit is equal to the preset temperature, the temperature of the laser heating unit is stabilized; and the heating temperature is maintained according to the experimental needs; thereby achieving accurate non-contact temperature control of the target area of ​​the experimental small animals.

[0011] Preferably, in step S01, the diameter of the target area ranges from 1 mm to 8 mm.

[0012] In step S01, due to the different sizes and shapes of the experimental animals and the different target locations, the distance between the target location to be heated and the laser heating unit is between 1mm-20mm, and it cannot be guaranteed that the distance is completely consistent in each experiment, which will cause the same output power of the laser heating unit to have different real-time temperatures for different target locations; therefore, for animal experiments with higher temperature control requirements, a more precise temperature control system is required.

[0013] Preferably, in step S02,

[0014] The laser heating unit includes a matching laser generator and a focusing lens; the position of the focusing lens is adjusted so that the laser energy emitted by the laser generator is concentrated on the target area to achieve non-contact heating. The relative position relationship between the laser generator, the focusing lens and the target area ensures efficient transmission of laser energy and precise heating of the target area.

[0015] The preset temperature value preferably ranges from 40°C to 52°C.

[0016] The heating temperature reached by the initial heating power of the laser heating unit is 2-3°C lower than the preset temperature; this avoids excessive temperature stimulation to the experimental animals due to the initial heating temperature of the laser heating unit being higher than the preset temperature, thereby affecting the experimental results; and if it is 2-3°C lower than the preset temperature, after the subsequent infrared temperature measurement unit accurately detects and feeds back to the control system unit, the control system unit gradually heats the laser heating unit in small steps, which will steadily raise the temperature to the preset temperature without causing excessive temperature stimulation exceeding the preset temperature to the experimental animals.

[0017] Preferably, in step S03,

[0018] When the infrared temperature measurement unit performs real-time temperature detection on the target area, the control system unit suspends the laser output of the laser heating unit, and resumes the laser output of the laser heating unit after the infrared temperature measurement unit completes a real-time temperature detection. By accurately controlling the laser heating unit by the control system unit, it is prevented that the laser output of the laser heating unit affects the authenticity of the real-time monitoring temperature of the target area detected by the infrared temperature measurement unit.

[0019] Preferably, in step S03, the control system unit regulates the output power temperature of the laser heating unit, specifically: when the real-time detected temperature is less than the preset temperature>3°C, the step-by-step temperature increase adjustment is performed according to a temperature increase step of 50% of the temperature difference; when the temperature difference between the real-time detected temperature and the preset temperature is less than 3°C, the step-by-step temperature increase adjustment is performed according to a temperature increase step of 0.1°C; when the difference between the real-time detected temperature and the preset temperature is less than 0.1°C, the control system adjustment is stopped to complete the temperature adjustment process; wherein, the temperature increase step time interval is 1s. Through the regulation of the above control system, when the real-time detected temperature is significantly different from the preset temperature, the temperature can be quickly increased by increasing the temperature by half (50%) of the difference temperature, and when the temperature is increased to the real-time detected temperature less than the preset temperature<3°C, the temperature is slowly and accurately increased by 0.1°C steps, avoiding the over-temperature stimulation caused by rapid temperature increase; and the entire adjustment process can be completed in 30s, and the final real-time monitoring temperature and the expected temperature achieve an accurate temperature control effect of less than 0.1°C.

[0020] Preferably, in step S04,

[0021] When the maintenance reaches a preset maintenance time, the laser heating unit is turned off, and the heating and temperature control process ends.

[0022] A non-contact temperature control system for small animal experiments, comprising:

[0023] A laser heating unit, which is used to perform non-contact heating on a target area by adjusting the output power and focusing distance of the laser generated by the laser heating unit;

[0024] Infrared temperature measurement unit monitors the temperature of the target area in real time and transmits the timely temperature signal to the control system;

[0025] The control system unit adjusts the output power and focusing distance of the laser heating unit according to the real-time temperature signal sent by the infrared temperature measuring unit by comparing the temperature signal with the target preset temperature, thereby realizing closed-loop control and ensuring that the temperature of the target area reaches the preset value.

[0026] Preferably, the laser heating unit is a non-contact laser heating unit.

[0027] Further preferably, the laser heating unit includes a laser generator and a focusing lens; the focusing lens is arranged at an adjustable distance at the front end of the laser generator, the laser generator generates laser to perform non-contact heating on the target area; the focusing lens focuses and adjusts the laser to concentrate its energy on the target area.

[0028] The infrared temperature measurement unit is preferably a non-contact infrared temperature measurement sensor.

[0029] Preferably, the control system unit is a control system based on an ATmega 168P single chip microcomputer.

[0030] The temperature control system of the present invention generates laser light from a laser generator, and concentrates the energy to the target area of ​​the experimental small animal through a focusing lens for non-contact heating; the infrared temperature sensor monitors the temperature of the target area in real time, and transmits the temperature signal to the control system based on the ATmega 168P single-chip microcomputer. The control system adjusts the output power of the laser generator according to the temperature signal to ensure that the temperature of the target area reaches a preset value. Through this process, the present invention realizes non-contact temperature stimulation and real-time and accurate temperature control.

[0031] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0032] 1. The non-contact temperature control method and system for small animal experiments of the present invention avoid the disadvantages of contact heating: Compared with traditional contact methods such as water bath heating, the present invention adopts a non-contact laser heating method to avoid direct contact between the skin and the heating medium. This non-contact heating method reduces the activation of mechanical receptors, reduces the heating range, and makes the heating more precise and local. It is particularly suitable for small animal experiments and can more effectively study the effects of temperature stimulation on experimental samples.

[0033] 2. The non-contact temperature control method and system for small animal experiments of the present invention achieve more accurate temperature control: Unlike common experimental temperature control stimulation devices, the non-contact temperature control device of the present invention uses an infrared temperature measurement module to monitor the temperature of the target skin in real time, rather than the temperature of the output end. This makes the temperature control more accurate and effectively avoids the error caused by the difference between the output end temperature and the target skin temperature. Through the control system based on the ATmega 168P single-chip microcomputer, precise control of parameters such as the preset temperature range and heating time can be achieved, further improving the reliability and accuracy of the experiment.

[0034] 3. The non-contact temperature control method for small animal experiments of the present invention adopts a method in which the heating temperature achieved by the initial heating power of the laser heating unit is 2-3°C lower than the preset temperature; and the control system unit receives the real-time monitoring temperature feedback of the infrared temperature measurement unit after startup, and gradually and accurately heats the temperature through a step heating method to achieve precise control of non-contact temperature control, and the temperature control accuracy error is within 0.1°C.

[0035] 4. The present invention uses a non-contact laser heating unit for heating, adopts laser as a heat source, and realizes non-contact heating of the target area by adjusting the output power and focusing distance of the laser; the laser heating unit adopted has the advantages of fast heating speed, local heating, and easy control. In the present invention, the relative position relationship between the laser generator, the focusing lens and the target area ensures the efficient transmission of laser energy and the precise heating of the target area. The present invention adopts an infrared temperature sensor to monitor the temperature of the target area in real time and transmit the temperature signal to the control system; the control system adjusts the output power and focusing adjustment of the laser heating unit according to the temperature signal to realize closed-loop control and ensure that the temperature of the target area reaches the preset value.

[0036] 5. The non-contact temperature control system for small animal experiments of the present invention adopts non-contact laser heating and real-time accurate infrared temperature measurement technology to achieve accurate temperature control under different distances, diameters, temperatures and durations. The system can be widely used in biological, medical, physical and other experimental fields to perform temperature stimulation on experimental samples to study the effect of temperature on sample performance and biological activity. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 A schematic flow chart of a non-contact temperature control method for small animal experiments provided by an embodiment of the present invention;

[0038] Figure 2 A schematic structural diagram of a non-contact temperature control system for small animal experiments provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0041] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element at the same time.

[0042] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or suggesting their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0043] At present, the existing temperature control system has the defects of using contact heating and the inability to accurately detect the temperature in real time. The embodiment of the present invention provides a non-contact temperature control method and system for small animal experiments. The temperature control device provided by the present invention adopts a laser heating method to avoid the disadvantages of contact heating; and the infrared temperature measurement module equipped with the device measures the real-time temperature of the target skin, so that the temperature control is more accurate.

[0044] Example 1

[0045] like Figure 1 As shown, a non-contact temperature control method for small animal experiments specifically includes the following steps:

[0046] S01. Fix the experimental animal so that the distance between the target area to be heated and the laser heating unit is between 1mm and 20mm;

[0047] S02. Setting a preset temperature, using a laser heating unit to heat the target area, and achieving non-contact heating of the target area by adjusting the output power and focusing distance of the laser heating unit;

[0048] S03. Use an infrared temperature measurement unit to accurately detect the temperature of the target area, and feed back the real-time detected temperature to the control system unit. The control system unit compares the difference between the real-time detected temperature and the preset temperature; the control system adjusts the output power of the laser heating unit;

[0049] S04. When the real-time detected temperature of the infrared temperature measuring unit is equal to the preset temperature, the temperature of the laser heating unit is stabilized; and the heating temperature is maintained according to the experimental needs; thereby achieving accurate non-contact temperature control of the target area of ​​the experimental small animals.

[0050] Preferably, in step S01, the diameter of the target area ranges from 1 mm to 8 mm.

[0051] In step S01, due to the different sizes and shapes of the experimental animals and the different target locations, the distance between the target location to be heated and the laser heating unit is between 1mm-20mm, and it cannot be guaranteed that the distance is completely consistent in each experiment, which will cause the same output power of the laser heating unit to have different real-time temperatures for different target locations; therefore, for animal experiments with higher temperature control requirements, a more precise temperature control system is required.

[0052] Preferably, in step S02,

[0053] The laser heating unit includes a matching laser generator and a focusing lens; the position of the focusing lens is adjusted so that the laser energy emitted by the laser generator is concentrated on the target area to achieve non-contact heating. The relative position relationship between the laser generator, the focusing lens and the target area ensures efficient transmission of laser energy and precise heating of the target area.

[0054] The preset temperature value preferably ranges from 40°C to 52°C.

[0055] The heating temperature reached by the initial heating power of the laser heating unit is 2-3°C lower than the preset temperature; this avoids excessive temperature stimulation to the experimental animals due to the initial heating temperature of the laser heating unit being higher than the preset temperature, thereby affecting the experimental results; and if it is 2-3°C lower than the preset temperature, after the subsequent infrared temperature measurement unit accurately detects and feeds back to the control system unit, the control system unit gradually heats the laser heating unit in small steps, which will steadily raise the temperature to the preset temperature without causing excessive temperature stimulation exceeding the preset temperature to the experimental animals.

[0056] Preferably, in step S03,

[0057] When the infrared temperature measurement unit performs real-time temperature detection on the target area, the control system unit suspends the laser output of the laser heating unit, and resumes the laser output of the laser heating unit after the infrared temperature measurement unit completes a real-time temperature detection. By accurately controlling the laser heating unit by the control system unit, it is prevented that the laser output of the laser heating unit affects the authenticity of the real-time monitoring temperature of the target area detected by the infrared temperature measurement unit.

[0058] Preferably, in step S03, the control system regulates the temperature of the laser heating unit, specifically: when the real-time detected temperature is less than the preset temperature>3°C, the step-by-step temperature increase adjustment is performed according to a temperature increase step of 50% of the temperature difference; when the temperature difference between the real-time detected temperature and the preset temperature is less than 3°C, the step-by-step temperature increase adjustment is performed according to a temperature increase step of 0.1°C; when the difference between the real-time detected temperature and the preset temperature is less than 0.1°C, the control system adjustment is stopped to complete the temperature adjustment process; wherein, the temperature increase step time interval is 1s. Through the regulation of the above control system, when the real-time detected temperature is significantly different from the preset temperature, the temperature can be quickly increased by increasing the temperature by half (50%) of the difference temperature, and when the temperature is increased to the real-time detected temperature less than the preset temperature<3°C, the temperature is slowly and accurately increased by 0.1°C steps, avoiding the over-temperature stimulation caused by rapid temperature increase; and the entire adjustment process can be completed in 30s, and the final real-time monitoring temperature and the expected temperature achieve an accurate temperature control effect of less than 0.1°C.

[0059] Preferably, in step S04,

[0060] When the maintenance reaches a preset maintenance time, the laser heating unit is turned off, and the heating and temperature control process ends.

[0061] like Figure 2 As shown, a non-contact temperature control system for small animal experiments includes:

[0062] A laser heating unit, which is used to perform non-contact heating on a target area by adjusting the output power and focusing distance of the laser generated by the laser heating unit;

[0063] Infrared temperature measurement unit monitors the temperature of the target area in real time and transmits the timely temperature signal to the control system;

[0064] The control system unit adjusts the output power and focusing distance of the laser heating unit according to the real-time temperature signal sent by the infrared temperature measuring unit by comparing the temperature signal with the target preset temperature, thereby realizing closed-loop control and ensuring that the temperature of the target area reaches the preset value.

[0065] Preferably, the laser heating unit is a non-contact laser heating unit.

[0066] Further preferably, the laser heating unit includes a laser generator and a focusing lens; the focusing lens is arranged at an adjustable distance at the front end of the laser generator, the laser generator generates laser to perform non-contact heating on the target area; the focusing lens focuses and adjusts the laser to concentrate its energy on the target area.

[0067] The infrared temperature measurement unit is preferably a non-contact infrared temperature measurement sensor.

[0068] Preferably, the control system unit is a control system based on an ATmega 168P single chip microcomputer.

[0069] The temperature control system of the present invention generates laser light from a laser generator, and concentrates the energy to the target area of ​​the experimental small animal through a focusing lens for non-contact heating; the infrared temperature sensor monitors the temperature of the target area in real time, and transmits the temperature signal to the control system based on the ATmega 168P single-chip microcomputer. The control system adjusts the output power of the laser generator according to the temperature signal to ensure that the temperature of the target area reaches a preset value. Through this process, the present invention realizes non-contact temperature stimulation and real-time and accurate temperature control.

[0070] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0071] 1. The non-contact temperature control method and system for small animal experiments of the present invention avoid the disadvantages of contact heating: Compared with traditional contact methods such as water bath heating, the present invention adopts a non-contact laser heating method to avoid direct contact between the skin and the heating medium. This non-contact heating method reduces the activation of mechanical receptors, reduces the heating range, and makes the heating more precise and local. It is particularly suitable for small animal experiments and can more effectively study the effects of temperature stimulation on experimental samples.

[0072] 2. The non-contact temperature control method and system for small animal experiments of the present invention achieve more accurate temperature control: Unlike common experimental temperature control stimulation devices, the non-contact temperature control device of the present invention uses an infrared temperature measurement module to monitor the temperature of the target skin in real time, rather than the temperature of the output end. This makes the temperature control more accurate and effectively avoids the error caused by the difference between the output end temperature and the target skin temperature. Through the control system based on the ATmega 168P single-chip microcomputer, precise control of parameters such as the preset temperature range and heating time can be achieved, further improving the reliability and accuracy of the experiment.

[0073] 3. The non-contact temperature control method for small animal experiments of the present invention adopts a method in which the heating temperature achieved by the initial heating power of the laser heating unit is 2-3°C lower than the preset temperature; and after startup, the control system unit monitors the temperature feedback in real time through the infrared temperature measurement unit, and gradually and accurately heats the room through a step heating method, thereby achieving precise control of non-contact temperature control, and the temperature control accuracy error is within 0.1°C.

[0074] 4. The present invention uses a non-contact laser heating unit for heating, adopts laser as a heat source, and realizes non-contact heating of the target area by adjusting the output power and focusing distance of the laser; the laser heating unit adopted has the advantages of fast heating speed, local heating, and easy control. In the present invention, the relative position relationship between the laser generator, the focusing lens and the target area ensures the efficient transmission of laser energy and the precise heating of the target area. The present invention adopts an infrared temperature sensor to monitor the temperature of the target area in real time and transmit the temperature signal to the control system; the control system adjusts the output power and focusing adjustment of the laser heating unit according to the temperature signal to realize closed-loop control and ensure that the temperature of the target area reaches the preset value.

[0075] 5. The non-contact temperature control system for small animal experiments of the present invention adopts non-contact laser heating and real-time accurate infrared temperature measurement technology to achieve accurate temperature control under different distances, diameters, temperatures and durations. The system can be widely used in biological, medical, physical and other experimental fields to perform temperature stimulation on experimental samples to study the effect of temperature on sample performance and biological activity.

[0076] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A non-contact temperature control method for small animal experiments, characterized in that: The specific steps include: S01. Fix the experimental animal so that the distance between the target area to be heated and the laser heating unit is between 1mm and 20mm; S02. Setting a preset temperature, using a laser heating unit to heat the target area, and achieving non-contact heating of the target area by adjusting the output power and focusing distance of the laser heating unit; S03. Use an infrared temperature measurement unit to accurately detect the temperature of the target area, and feed back the real-time detected temperature to the control system unit. The control system unit compares the difference between the real-time detected temperature and the preset temperature; the control system adjusts the output power of the laser heating unit; S04. When the real-time detected temperature of the infrared temperature measuring unit is equal to the preset temperature, the temperature of the laser heating unit is stabilized; and the heating temperature is maintained according to the experimental needs; thereby achieving accurate non-contact temperature control of the target area of ​​the experimental small animals.

2. The non-contact temperature control method for small animal experiments according to claim 1, characterized in that: In step S01, the diameter of the target area ranges from 1 mm to 8 mm.

3. The non-contact temperature control method for small animal experiments according to claim 1, characterized in that: In step S02, the laser heating unit includes a matching laser generator and a focusing lens; the position of the focusing lens is adjusted so that the laser energy emitted by the laser generator is concentrated on the target location to achieve non-contact heating.

4. The non-contact temperature control method for small animal experiments according to claim 1, characterized in that: In step S02, the preset temperature value preferably ranges from 40°C to 52°C.

5. The non-contact temperature control method for small animal experiments according to claim 1, characterized in that: In step S02, the heating temperature reached by the initial heating power of the laser heating unit is 2-3°C lower than the preset temperature.

6. The non-contact temperature control method for small animal experiments according to claim 1, characterized in that: In step S03, when the infrared temperature measurement unit performs real-time temperature detection on the target area, the control system unit suspends the laser output of the laser heating unit, and resumes the laser output of the laser heating unit after the infrared temperature measurement unit completes a real-time temperature detection.

7. The non-contact temperature control method for small animal experiments according to claim 1, characterized in that: In step S03, the control system unit regulates the output power temperature of the laser heating unit, specifically: when the real-time detected temperature is less than the preset temperature by more than 3°C, the step-by-step temperature increase adjustment is performed according to a temperature increase step of 50% of the temperature difference; when the temperature difference between the real-time detected temperature and the preset temperature is less than 3°C, the step-by-step temperature increase adjustment is performed according to a temperature increase step of 0.1°C; when the difference between the real-time detected temperature and the preset temperature is less than 0.1°C, the adjustment of the control system is stopped to complete the temperature adjustment process; wherein, the temperature increase step time interval is 1s.

8. The non-contact temperature control method for small animal experiments according to claim 1, characterized in that: In step S04, when the maintenance reaches a preset maintenance time, the laser heating unit is turned off, and the heating and temperature control process ends.

9. A non-contact temperature control system for small animal experiments, characterized in that: The temperature control method according to any one of claims 1 to 8 comprises: A laser heating unit, which is used to perform non-contact heating on a target area by adjusting the output power and focusing distance of the laser generated by the laser heating unit; Infrared temperature measurement unit monitors the temperature of the target area in real time and transmits the timely temperature signal to the control system; The control system unit adjusts the output power and focusing distance of the laser heating unit according to the real-time temperature signal sent by the infrared temperature measuring unit by comparing the temperature signal with the target preset temperature, thereby realizing closed-loop control and ensuring that the temperature of the target area reaches the preset value.

10. The non-contact temperature control system for small animal experiments according to claim 9, characterized in that: The laser heating unit comprises a laser generator and a focusing lens; the focusing lens is arranged at the front end of the laser generator with an adjustable distance, the laser generator generates laser to perform non-contact heating on the target area; the focusing lens focuses and adjusts the laser to concentrate its energy on the target area; The control system unit is a control system based on ATmega 168P single chip microcomputer.