Selenium-enriched yeast production method
By simplifying the fermentation process of selenium-rich yeast and using specific substrates and fermentation steps, the problems of complex processes and environmental pollution are solved, efficient and environmentally friendly production is achieved, and product quality is improved.
Patent Information
- Application Number
- CN202410980787.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-22
- Publication Date
- 2025-03-04
AI Technical Summary
The existing selenium-rich yeast production process is complex and has environmental pollution problems, including the discharge of waste slag and wastewater.
Using a simple fermentation process, the production of filtration and emissions is achieved through specific matrix and fermentation steps, including nutrient solution preparation, yeast culture medium preparation, selenium-rich yeast preparation and fermentation.
It greatly simplifies production equipment and operations, improves production efficiency, avoids environmental pollution, reduces production costs, and ensures product quality.
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Abstract
Description
Technical Field
[0001] The invention relates to a method for producing selenium-enriched yeast. Background Art
[0002] Selenium-enriched yeast mainly plays the role of supplementing the selenium element required by the human body. It can provide the human body with the appropriate amount of selenium needed to maintain normal physiological functions and participate in antioxidant reactions, immune regulation and other processes. The existing selenium-enriched yeast production process mainly includes slant strain culture, primary liquid seed culture, secondary liquid seed culture, fermentation tank culture, separation, drying, etc. In the existing traditional production process, its fermentation culture is all liquid fermentation. The main problems include complex fermentation production process, and the discharge of waste residues, wastewater and other waste materials, causing environmental pollution, etc. Summary of the invention
[0003] The purpose of the present invention is to provide a method for producing selenium-enriched yeast in view of the problems existing in the above-mentioned prior art. The method for producing selenium-enriched yeast is simple, efficient and environmentally friendly.
[0004] The technical solution of the selenium-enriched yeast production method of the present invention comprises the following steps: S1. Preparation of nutrient components of culture medium: A nutrient solution with a selenium concentration of 3000 μg / ml and a pH value of 4.8-6.8 is prepared by using selenite, sugar, purified water and amino acids in a certain ratio; S2. Preparation of yeast culture medium: The nutrient solution prepared in step S1 is added with 0.5-5% dry yeast at a temperature of 28-30° C. to activate the yeast culture medium; S3, preparation of selenium-enriched yeast: Mix flour and the yeast culture medium obtained in step S2 in a certain ratio, and ferment to obtain selenium-enriched yeast; S4. Fermentation: The selenium-enriched yeast obtained in step S3 is added to the nutrient solution in a certain proportion and stirred, and then flour is added to knead the dough, and then sealed and fermented. After that, the kneading and sealed fermentation of the sealed and fermented dough are repeated several times.
[0005] The mass ratio of the flour in step S3 to the yeast culture medium obtained in step S2 is 6:4.
[0006] Since the present invention adopts a special matrix and a unique fermentation process method, the production process is extremely simple and stable, the product quality is high, the production equipment and operation are greatly simplified, the production efficiency is doubled, and there is no emission and no need for filtration, thus avoiding the pollution problem of the environment caused by the production of selenium-enriched yeast, improving the production operation environment, and correspondingly reducing the production cost. DETAILED DESCRIPTION
[0007] In order to further understand the technical solution of the present invention, the present invention is now further clearly and completely described through the following examples. The description of its specific implementation method is only for the convenience of explaining the principle of the technical solution of the present invention. Those skilled in the art can make various modifications or improvements based on the basic idea of the present invention, but as long as they do not deviate from the basic technical idea of the present invention, they are all within the scope of the present invention.
[0008] The selenium-enriched yeast production method of the invention comprises: preparing nutrient components, preparing yeast culture medium, preparing selenium-enriched yeast, fermenting selenium-enriched yeast and the like.
[0009] The selenium-enriched yeast fermentation of the present invention mainly comprises the following steps: adding the obtained selenium-enriched yeast to the nutrient solution and flour in a certain proportion, stirring and sealing fermentation in turn, respectively, and then repeating the stirring fermentation and sealing fermentation. That is, firstly, the selenium-enriched yeast and the nutrient solution are mixed and stirred and fermented for a certain period of time in a certain proportion, and then a certain mass ratio of flour is added to stir and ferment for a certain period of time and sealed fermentation for a certain period of time, and then the dough that has been sealed and fermented for a certain period of time is subjected to multiple stirring fermentation and sealed fermentation without adding materials, and the stirring fermentation includes stirring with a stirrer and / or kneading and fermenting with a dough mixer.
[0010] In Example 1 of the present invention, the specific production process steps include: S1. Nutritional base nutritional ingredients preparation: The mass ratio of selenate or selenite, amino acid and white sugar culture medium is: 5 grams of selenite, 80 grams of white sugar, 80-200 grams of amino acid with a pH value of 3.5-4.5 and 800 milliliters of purified water; the preparation method is: 5 grams of selenite and 80 grams of white sugar are added into 800 milliliters of purified water to dissolve, then 80-200 grams of amino acid with a pH value of 3.5-4.5 is added, and the selenium concentration is adjusted to 3000 μg / ml and the pH value is 4.8-6.8 to prepare a nutrient solution; S2. Preparation of yeast culture medium: Add 0.5-5% dry yeast to the nutrient solution prepared in step S1 at a temperature of 28-30° C., activate the dry yeast for 30 minutes, and obtain an activated yeast culture medium.
[0011] S3, preparation of selenium-enriched yeast: The flour and the yeast culture medium activated in step S2 are put into a dough mixer in a mass ratio of 6:4 for 20-30 minutes, and the dough is fermented for 6-8 hours to obtain selenium-enriched yeast. In actual production operation, the dough can be put into a fermentation basin for fermentation for 6-8 hours.
[0012] S4. Fermentation: The fermentation production of selenium-enriched yeast of the present invention mainly comprises the following steps: adding nutrient solution to selenium-enriched yeast in a certain proportion and stirring for a certain time, then adding it to flour in a certain proportion, kneading the dough for a certain time, and then sealing and fermenting the sealed fermented dough for several times.
[0013] The stirring, kneading and fermenting of the present invention are carried out in a sterile environment space.
[0014] The specific fermentation process steps of this embodiment include: S4-1, adding the selenium-enriched yeast obtained in step S3 to the nutrient solution obtained in step S1, stirring and mixing for about 30 minutes to obtain a culture solution, adding the obtained culture solution to flour, and kneading the dough with a dough mixer for 20 to 30 minutes, and then kneading the dough for 20 to 30 minutes for 30 to 35 minutes. o C. Sealed fermentation for 8-12 hours. The mass ratio of the culture solution to the flour is 6:4.5. The mass ratio of selenium-enriched yeast to the nutrient solution can be 5-8:100. In actual operation, sealed fermentation can be done by putting the dough into a stainless steel barrel with a lid for sealed fermentation. The dough loading amount of the stainless steel barrel can be controlled at about 50% of the barrel volume, and the barrel lid is covered for sealed fermentation. The volume of the dough after sealed fermentation for 8-12 hours will expand to more than 80%.
[0015] S4-2, take out the dough fermented in step S4-1 and put it into a dough mixer for 20 to 30 minutes, and then seal and ferment at a temperature of 30 to 35 o C, ferment for 8-12 hours. In the specific operation of sealed fermentation, the dough that has been kneaded in the dough mixer for 20-30 minutes can be taken out and placed in a stainless steel barrel with a lid for fermentation. The volume of the dough after fermentation will expand by 50% or more.
[0016] S4-3, repeat the dough kneading and fermentation operation for several times according to the method of step S4-2, until the volume of the fermented dough does not increase and the color is light yellow or brownish yellow, take out the dough sample for testing, when the organic selenium accounts for more than 97% of the total selenium mass, the selenium content is more than 2000 mg / kg, it is qualified, the fermentation is completed, take out the fermented dough, dry it, put it into a grinder, crush it, inactivate and sterilize it, and then pack it for storage. The specific fermentation process operation number of step S4-3 can be 5 to 6 times. No other materials need to be added during the entire fermentation process after the first sealed fermentation.
[0017] The product produced by the selenium-enriched yeast production method of the present invention has been inspected by the Food Inspection and Testing Institute of Jiangxi Provincial Inspection, Testing and Certification Institute, and the inspection results are shown in the following inspection report (inspection sample name: selenium-enriched yeast, inspection report number WT2023050192, inspection date: 2023.5.25-2023.6.13):
[0018]
[0019] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.
Claims
1. A method for producing selenium-enriched yeast, characterized in that: The steps include: S1. Preparation of nutrient components of culture medium: A nutrient solution with a selenium concentration of 3000 μg / ml and a pH value of 4.8-6.8 is prepared by using selenite, sugar, purified water and amino acids in a certain ratio; S2. Preparation of yeast culture medium: The nutrient solution prepared in step S1 is added with 0.5-5% dry yeast at a temperature of 28-30° C. to activate the yeast culture medium; S3, preparing selenium-enriched yeast: mixing flour and the yeast culture medium obtained in step S2 in a certain ratio, and fermenting to obtain selenium-enriched yeast; S4. Fermentation: The selenium-enriched yeast obtained in step S3 is added to the nutrient solution in a certain proportion and stirred, and then flour is added to knead the dough, and then sealed and fermented. After that, the kneading and sealed fermentation of the sealed and fermented dough are repeated several times.
2. The method for producing selenium-enriched yeast according to claim 1, characterized in that: The mass ratio of the flour in step S3 to the yeast culture medium obtained in step S2 is 6:4.