Plant whitening and sunscreen composition as well as preparation method and application thereof

Abstract

The invention discloses a plant whitening and sunscreen composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The invention provides a plant whitening and sunscreen composition. The plant whitening and sunscreen composition is prepared from the following components in parts by weight: 0.5 to 5 parts of P / O / W composite coating body, 0.1 to 3 parts of bakuchiol and 0.1 to 3 parts of thyme extract. According to the invention, pterostilbene and verbascoside are stabilized in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite inclusion body, and the P / O / W composite inclusion body is mixed with the bakuchiol and thyme extract in specific parts by weight, so that on one hand, safe and efficient whitening can be realized by repairing, inhibiting melanin generation, inhibiting melanin transport and promoting melanin autophagy multi-channel synergistic interaction; on the other hand, whitening and sunscreen can be safely and efficiently achieved, the effect of external protection and internal whitening is achieved, the defensive barrier of the skin can be synergistically reinforced, and the problems of dark skin of sensitive skin and the like are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a plant whitening and sunscreen composition and a preparation method and application thereof. Background Art

[0002] People with sensitive skin have a thinner stratum corneum, and the barrier function of the epidermis is easily damaged, making the skin prone to allergic reactions under physiological or pathological conditions. Ultraviolet radiation and inflammation are the main factors that activate melanocytes to produce melanin. Sensitive skin is more susceptible to sensitization and dark spots due to ultraviolet radiation than normal skin due to damaged skin barrier, so it is more important to pay attention to sun protection and whitening. Sunscreens and whitening agents in cosmetics are two types of raw materials with more controversial safety issues. Sensitive skin is more susceptible to irritation from sunscreens and whitening agents, resulting in adverse reactions.

[0003] Current whitening sunscreens have the following shortcomings: (1) Current whitening sunscreen technologies mostly focus on the combination of whitening agents and sunscreens. They do not take into account the characteristics of sensitive skin, and consider efficacy while ignoring safety. (2) For people with sensitive skin, simply combining whitening and sunscreen is not enough. It is necessary to first repair the damaged barrier and strengthen the skin's defense function in order to achieve good whitening and sunscreen. (3) In the research on whitening sunscreens for sensitive skin, few people have considered the synergistic effect of the combination of the two.

[0004] Therefore, how to provide a plant-based whitening sunscreen composition that reduces the irritation of the formula while achieving effective sunscreen and whitening effects through ingredient screening and reasonable compatibility has broad application prospects. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a plant whitening sunscreen composition that achieves effective sunscreen and whitening effects while reducing the irritation of the formula through ingredient screening and reasonable compatibility, and has broad application prospects.

[0006] To achieve the above-mentioned purpose, the technical scheme adopted by the present invention is: a plant whitening sunscreen composition, comprising the following components in parts by weight: 0.5-5 parts of a P / O / W composite carrier, 0.1-3 parts of bakuchiol, and 0.1-3 parts of a thyme extract; the P / O / W composite carrier comprises the following three phases in percentage by weight: 8-20% of an alcohol phase, 20-35% of an oil phase, and 50-70% of an aqueous phase; the alcohol phase comprises verbascoside, pterostilbene, and a polyol; the oil phase comprises an emulsifier and oils; The aqueous phase comprises solid particles and deionized water; wherein the polyol comprises at least two of butylene glycol, glycerol, dipropylene glycol, propylene glycol, and PEG400; the oil comprises at least one of butyl octanol salicylate, C12-15 alcohol benzoate, isononyl isononanoate, and dicaprylyl carbonate; the solid particles are at least one of cellulose and modified starch; and in the P / O / W composite carrier, the mass percentage of verbascoside is 0.1-2%, and the mass percentage of pterostilbene is 0.1-5%.

[0007] The present invention stabilizes pterostilbene and verbascoside in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite encapsulation carrier. On the one hand, pterostilbene and verbascoside have good activity, but poor stability, especially poorer stability under light conditions. The present invention stabilizes pterostilbene and verbascoside in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite encapsulation carrier, which can better dissolve pterostilbene and verbascoside, and at the same time, specific weight portions of bakuchiol and thyme extract can well block the influence of light on the stability of these components, thereby maintaining the biological activity of each component to the maximum extent. On the second hand, the encapsulation form of the P / O / W composite encapsulation carrier solves the defects existing in the application of components, such as poor stability, low activity in actual application, etc., improves the utilization rate, and is more suitable for sensitive skin. At the same time, the present invention mixes the P / O / W composite carrier encapsulating pterostilbene and verbascoside with specific weight portions of bakuchiol and European thyme extract, which can achieve safe and efficient whitening by repairing, inhibiting melanin production, inhibiting melanin transport and promoting melanin autophagy multi-pathway synergistic enhancement; it can also achieve safe and efficient whitening and sun protection, achieve the effect of "external protection and internal whitening", and can synergistically strengthen the skin's defensive barrier and improve the problems of dull sensitive skin. In the third aspect, the encapsulation of the specific P / O / W composite carrier of the present invention, combined with the plant whitening and sunscreen composition formed by specific weight portions of bakuchiol and European thyme extract, can achieve long-term efficacy after transdermal absorption during the action process.

[0008] Preferably, the weight ratio of the P / O / W composite carrier, bakuchiol and thyme extract is P / O / W composite carrier: bakuchiol: thyme extract = 1: (0.1-0.8): (0.1-0.8).

[0009] Preferably, in the plant whitening and sunscreen composition, the mass percentage of the P / O / W composite carrier is 45-60%.

[0010] The inventors found in actual experiments that the weight of the components in the plant whitening and sunscreen composition will affect the performance of the product. When the weight of the components is further selected to be within the above range, the obtained product has a better whitening and sunscreen effect.

[0011] Preferably, the polyol is a mixture of glycerol and dipropylene glycol, and the weight ratio of the glycerol to dipropylene glycol is (2-8): (2-8).

[0012] Preferably, the dielectric constant of the grease is 2-4.

[0013] Further preferably, the polyol is a mixture of glycerol and dipropylene glycol, the weight ratio of the glycerol to dipropylene glycol is 1:1, and the oil is isononyl isononanoate.

[0014] The inventors found in actual experiments that the selection of the above-mentioned specific polyols and oils can better prepare the P / O / W composite packaging carrier, taking into account both the utilization of the active ingredients and the stability of the composite packaging carrier.

[0015] Preferably, the method for preparing the P / O / W composite carrier comprises the following steps:

[0016] S1, dissolving verbascoside and pterostilbene in polyol to prepare an inner alcohol phase;

[0017] S2, adding an emulsifier to the oil to obtain an oil phase;

[0018] S3, adding the inner alcohol phase to the oil phase, and homogenizing and dispersing to obtain P / O colostrum;

[0019] S4, adding solid particles into deionized water to disperse and obtain an aqueous phase;

[0020] S5. Add the P / O colostrum into the aqueous phase and disperse it homogeneously to obtain a P / O / W composite carrier.

[0021] Preferably, in step S1, the weight ratio of verbascoside, pterostilbene and polyol is verbascoside: pterostilbene: polyol=(0.1-2):(0.1-5):10;

[0022] And / or, in step S2, the weight ratio of oil to emulsifier is oil: emulsifier = (1-10): 1; and / or, in step S3, the weight ratio of alcohol phase to oil phase is alcohol phase: oil phase = (2-4): (6-8); and / or, in step S4, the weight ratio of solid particles to deionized water is solid particles: deionized water = 1: (10-30); and / or, in step S5, the weight ratio of P / O colostrum to water phase is P / O colostrum: water phase = (2-5): (5-8).

[0023] Preferably, in step S2, the emulsifier is a polyglycerol emulsifier, and preferably, the polyglycerol emulsifier is at least one of polyglyceryl-3 diisostearate, polyglyceryl-2 triisostearate, and polyglyceryl-2 dipolyhydroxystearate.

[0024] In addition, the present invention provides the use of the plant whitening and sunscreen composition in the preparation of skin products.

[0025] Furthermore, the present invention provides a skin product, which includes the plant whitening sunscreen composition; the skin product includes one of sunscreen milk, sunscreen lotion, sunscreen cream, sunscreen liquid, sunscreen powder, sunscreen spray, sunscreen gel, isolation cream, isolation milk, isolation lotion, and BB cream.

[0026] Preferably, the skin product comprises the following components in percentage by mass: 0.03-13% of plant whitening sunscreen composition, 2-30% of chemical sunscreen and / or inorganic ultraviolet blocker, 5-40% of cosmetic base, and the balance of water.

[0027] Preferably, the chemical sunscreen is at least one of bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl triazone, polysilicone-15, methylene bis-benzotriazolyl tetramethylbutylphenol, terephthalylidene dicamphor sulfonic acid, drometrizole trisiloxane, ethylhexyl methoxycinnamate, and ethylhexyl salicylate; and the inorganic UV blocker is at least one of zinc oxide and titanium dioxide.

[0028] Preferably, the cosmetic matrix includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, a flavor, and a pH regulator. Optionally, the thickener includes at least one of carbomer, xanthan gum, acrylate / C10-30 alkyl acrylate cross-linked polymer, and sodium polyacrylate, the moisturizer includes at least one of trehalose, sodium hyaluronate, erythritol, glyceryl polyether-26, xylitol glucoside, tremella extract, and β-glucan, the emulsifier includes at least one of hydrogenated lecithin, cetearyl glucoside, cetearyl alcohol polyether-20, polyglyceryl-10 stearate, and cetearyl alcohol polyether-20, the pH regulator includes at least one of arginine, triethanolamine, and sodium hydroxide, and the preservative is phenoxyethanol, sodium benzoate, and a moisturizer with preservative properties such as p-hydroxyacetophenone and capryloyl hydroxyvaleric acid.

[0029] Preferably, the present invention provides a whitening sunscreen lotion / cream comprising the following components in percentage by mass:

[0030] Deionized water balance, carbomer 0.1-0.5%, disodium EDTA 0.01-0.05%, butanediol 1-5%;

[0031] MONTANOV L (C14-22 alcohol, C12-20 alkyl glucoside) 1-5%, cetearyl alcohol 0.5-1.5%, diisopropyl sebacate 3-8%, dicaprylyl carbonate 3-8%, isononyl isononanoate 1-5%, bis-ethylhexyloxyphenol methoxyphenyl triazine 3-8%, ethylhexyl triazone 1-5%, drometrizole trisiloxane 1-5%, polysilicone-15 1-5%, titanium dioxide 1-3%;

[0032] 0.03-13% of plant whitening and sunscreen composition; 0.05-0.5% of essence; and 0.5-1% of preservative.

[0033] Optionally, the preservative is at least one of phenoxyethanol and sodium benzoate.

[0034] The present invention also provides a method for preparing the whitening sunscreen lotion / cream, comprising the following steps:

[0035] (1) Mix deionized water, carbomer, disodium EDTA, and butylene glycol components evenly, heat to 75-85° C., and stir until completely dissolved;

[0036] (2) Mix MONTANOV L (C14-22 alcohol, C12-20 alkyl glucoside), cetearyl alcohol, diisopropyl sebacate, dicaprylyl carbonate, isononyl isononanoate, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl triazone, drometrizole trisiloxane, polysilicone-15, and titanium dioxide, heat to 75-85° C., and stir until completely dissolved;

[0037] (3) Add the components in step (1) to the components in step (2), homogenize for 5-20 minutes, cool to 40-45° C., add the plant whitening sunscreen composition, flavor and preservative, stir and cool to below 38° C., and obtain the whitening sunscreen lotion / cream.

[0038] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention stabilizes pterostilbene and verbascoside in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite encapsulation carrier. On the one hand, pterostilbene and verbascoside have good activity, but poor stability, especially worse stability under light conditions; the present invention stabilizes pterostilbene and verbascoside in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite encapsulation carrier, which can better dissolve pterostilbene and verbascoside, and at the same time, specific weight portions of bakuchiol and thyme extract can well block the influence of light on the stability of these components, thereby maintaining the biological activity of each component to the maximum extent; on the second hand, the encapsulation form of the P / O / W composite encapsulation carrier solves the defects existing in the application of components, such as poor stability, low activity in actual application, etc., improves the utilization rate, and is more suitable for sensitive skin. At the same time, the present invention mixes the P / O / W composite carrier encapsulating pterostilbene and verbascoside with specific weight portions of bakuchiol and European thyme extract, which can achieve safe and efficient whitening by repairing, inhibiting melanin production, inhibiting melanin transport and promoting melanin autophagy multi-pathway synergistic enhancement; it can also achieve safe and efficient whitening and sun protection, achieve the effect of "external protection and internal whitening", and can synergistically strengthen the skin's defensive barrier and improve the problems of dull sensitive skin. In the third aspect, the encapsulation of the specific P / O / W composite carrier of the present invention, combined with the plant whitening and sunscreen composition formed by specific weight portions of bakuchiol and European thyme extract, can achieve long-term efficacy after transdermal absorption during the action process. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is the HPLC standard curve of pterostilbene;

[0040] Figure 2 HPLC chart of the content of W3 in the pterostilbene P / O / W composite carrier;

[0041] Figure 3 This is the HPLC chart of the content of W2 part in the pterostilbene P / O / W composite packaging carrier. DETAILED DESCRIPTION

[0042] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0043] The raw materials used in the embodiments and comparative examples are described below, but are not limited to these materials:

[0044] Polyol-1: a mixture of glycerol and dipropylene glycol, the weight ratio of glycerol to dipropylene glycol = 1:1;

[0045] Polyol-2: a mixture of glycerol and dipropylene glycol, with a weight ratio of glycerol to dipropylene glycol = 2:8;

[0046] Polyol-3: a mixture of glycerol and dipropylene glycol, with a weight ratio of glycerol to dipropylene glycol = 8:2;

[0047] Polyol-4: a mixture of glycerol and dipropylene glycol, the weight ratio of glycerol to dipropylene glycol = 1:9;

[0048] Polyol-5: glycerol;

[0049] Polyol-6: Butanediol;

[0050] Oil-1: Isononyl Isononanoate

[0051] Oil-2: C12-15 Alkyl Benzoate

[0052] Grease-3: White oil (<2)

[0053] Oil-4: Castor oil (>4)

[0054] Bakuchiol: Purchased from Guangdong Yue Microbiology Technology Co., Ltd.

[0055] Thyme extract: Japan Ichimaru Natural Beauty Co., Ltd.;

[0056] Verbascoside: Shanghai source leaf;

[0057] Pterostilbene: Sabinsa (Nanjing) Co., Ltd.;

[0058] Examples 1-12 and Comparative Examples 1-9

[0059] The components and weight proportions of the P / O / W composite carrier of the present invention are shown in Table 1 below. The preparation method of the P / O / W composite carrier of the present invention comprises the following steps:

[0060] S1, dissolving verbascoside and pterostilbene in polyol to prepare an inner alcohol phase;

[0061] S2, adding an emulsifier to the oil to obtain an oil phase;

[0062] S3, adding the inner alcohol phase to the oil phase, and homogenizing and dispersing to obtain P / O colostrum;

[0063] S4, adding solid particles into deionized water to disperse and obtain an aqueous phase;

[0064] S5. Add the P / O colostrum into the aqueous phase and disperse it homogeneously to obtain a P / O / W composite carrier.

[0065] The emulsifier used in the preparation method of the P / O / W composite carrier of the present invention is polyglycerol-3 diisostearate, and the solid particles used are octenyl succinic anhydride modified starch. PC / PURE, the emulsifier and the solid particles of the present invention are conventionally selected and can be conventionally selected by those skilled in the art.

[0066] Among them, the P / O / W composite package carrier-1 in Table 1 is omitted in the table and filled in P / O / W-1. Similarly, the total weight parts of the P / O / W composite package carrier in Table 1 is 100 parts.

[0067] The present application provides a plant whitening sunscreen composition, the components and weight portions of the plant whitening sunscreen composition are shown in Tables 2 and 3 below, and the preparation method of the plant whitening sunscreen composition is: P / O / W composite package carrier, bakuchiol, and thyme extract are mixed evenly to obtain the plant whitening sunscreen composition. When preparing the plant whitening sunscreen composition of the comparative example, if there is no relevant component, then it is not necessary to add it, and if there are other components, they are also added together and mixed evenly.

[0068] Table 1

[0069]

[0070] Table 2

[0071]

[0072]

[0073] Table 3

[0074]

[0075] Comparative Example 10

[0076] A plant whitening and sunscreen composition, comprising the following components in parts by weight: 0.03 parts of pterostilbene, 0.06 parts of verbascoside, 1 part of bakuchiol, and 1 part of thyme extract;

[0077] The preparation method of the plant whitening sunscreen composition comprises the following steps: evenly mixing pterostilbene, verbascoside, bakuchiol and thyme extract to obtain the plant whitening sunscreen composition.

[0078] Application examples 1-13, comparative application examples 1-10, blank application examples

[0079] Application Examples 1-13, Comparative Application Examples 1-10 and Blank Application Examples of the present invention provide a whitening sunscreen lotion / cream, the components (mass percentage) of the whitening sunscreen lotion / cream are shown in Table 4; wherein the plant whitening sunscreen compositions in Application Examples 1-12 are respectively the plant whitening sunscreen compositions prepared in Examples 1-12, and the plant whitening sunscreen compositions in Comparative Application Examples 1-10 are respectively the plant whitening sunscreen compositions prepared in Comparative Examples 1-10; for example, the plant whitening sunscreen composition used in Application Example 1 is the plant whitening sunscreen composition prepared in Example 1, the plant whitening sunscreen composition used in Application Example 2 is the plant whitening sunscreen composition prepared in Example 2, the plant whitening sunscreen composition used in Comparative Application Example 1 is the plant whitening sunscreen composition prepared in Comparative Example 1, and the rest can be deduced by analogy.

[0080] The plant whitening sunscreen composition used in Application Example 13 is the plant whitening sunscreen composition prepared in Example 1.

[0081] Table 4

[0082]

[0083]

[0084] Performance Test-1 Safety Evaluation.

[0085] Test method: human patch test

[0086] Testing process: 30 volunteers were recruited and the safety of the product was tested using a qualified spot tester in accordance with the methods specified in the Technical Specifications for Safety of Cosmetics.

[0087] Using the closed patch test method, equal amounts (0.020mL-0.025mL) of test samples (i.e., Application Examples 1-13, Comparative Application Examples 1-10, and Blank Application Examples) were placed in a specific patch tester, which was applied to the volunteer's arm with a low-allergenic tape, and gently pressed to evenly apply it to the skin for 24 hours; after 24 hours, the patch tester was removed, and the skin reaction was observed after 0.5h, 24h, and 48h, and the 48h skin results were recorded. The levels of adverse skin reactions are shown in Table 5 below, and the test results are shown in Table 6 below.

[0088] Table 5

[0089]

[0090] Table 6

[0091]

[0092]

[0093] The results of human patch tests show that the whitening sunscreen lotions / creams provided by Application Examples 1-13, Comparative Application Examples 1-10 and Blank Application Examples of the present invention are safe for use on human skin.

[0094] Performance test-2 Sun protection performance test

[0095] Using a sunlight simulator, a sun protection test was conducted on 5 volunteers according to the sun protection index determination method in the "Technical Specifications for Safety of Cosmetics".

[0096] Specifically: Determine the MED value of the volunteers in advance. After the back is divided into sections, one section is not coated with the sample, and the rest of the parts are coated with (2.00±0.05) mg / cm 2 Apply the sample with a fingertip and wait for 30 minutes. Then irradiate with a solar simulator and record the MED value of the sample after application. Calculate the SPF by calculating the MED value of the applied sample and the MED value of the unapplied sample.

[0097] The following are the test results, as shown in Table 7.

[0098] Table 7

[0099]

[0100]

[0101] It can be seen from the above table that the sunscreen product prepared using the whitening sunscreen composition prepared by the embodiment of the present invention has a very good SPF value of more than 30 and has a very good sunscreen effect.

[0102] Performance test-3 Encapsulation efficiency test

[0103] Test process: Draw the HPLC standard curve of pterostilbene. The chromatographic conditions are C18 column, mobile phase 65% potassium dihydrogen phosphate aqueous solution (10mmol / L) + 35% acetonitrile, flow rate 0.8ml / min, detection wavelength 306nm. Standard curve: y = 7.6789x + 0.9313, pterostilbene has a good linear relationship between 1-100ppm.

[0104] Accurately measure 1 ml of the P / O / W composite carrier AK prepared by the present invention, centrifuge (10000 r / 15 min), take the bottom liquid, and measure it by HPLC to obtain the free pterostilbene content, which is recorded as W2. After soaking in 5 ml of DMSO solution, ultrasonically crush and filter, measure the pterostilbene content by HPLC and record it as W3.

[0105] Encapsulation efficiency = (W3-W2) / W3*100%.

[0106] in, Figure 1 This is the HPLC standard curve of pterostilbene; Figure 2 HPLC chart of the content of W3 in the pterostilbene P / O / W composite carrier; Figure 3 This is the HPLC chart of the content of W2 part in the pterostilbene P / O / W composite packaging carrier.

[0107] The following are the encapsulation efficiency test results. P / O / W composite carrier-1 is omitted in the table and filled in P / O / W-1, and so on, as shown in Table 8:

[0108] Table 8

[0109]

[0110]

[0111] It can be seen from the above table that the encapsulation rate of the P / O / W composite carrier of the present invention is above 70%, and has a very good encapsulation effect.

[0112] Performance Test-4: Tyrosinase Inhibition

[0113] Tyrosinase inhibition was tested using an in vitro mushroom tyrosinase assay as follows:

[0114] 1. Solution preparation: (1) 100U / mL tyrosinase solution: dilute 25KU tyrosinase with PBS buffer to a 25mL brown volumetric flask, dilute to the mark, divide into 5 5mL centrifuge tubes and store in a -14℃ refrigerator. When taking, take 1mL of 1000U tyrosinase solution and dilute 10 times to obtain 100U tyrosinase solution; (2) 5% L-DOPA solution: accurately weigh 0.0125g of L-DOPA, place in a 25mL brown volumetric flask, dilute to the mark with pH7.4 PBS buffer, and prepare for use. (3) Test sample preparation: dissolve the sample with DMSO at a ratio of 1:1, and then filter with a filter membrane to obtain the test sample.

[0115] Test: Prepare 5 groups of sample tubes and blank control tubes according to the solutions in tubes A, B, C, and D as follows. Add 0.5 mL of 5% L-DOPA solution to each tube, react at 37°C for 30 minutes, measure the absorbance of the mixed solution at 475 nm, and repeat 3 times for each sample.

[0116] The solutions in test tubes A, B, C, and D are as follows: tube A: 3 mL PBS buffer + 0.5 mL tyrosinase; tube B: 3.5 mL PBS buffer; tube C: 1 mL sample solution + 2 mL PBS buffer + 0.5 mL tyrosinase; tube D: 1 mL sample solution + 2.5 mL PBS buffer.

[0117] Data analysis: SPSS20.0 and Excel 2020 software were used for statistical analysis. The data were first tested for normal distribution using the single-sample "KS" test and homogeneity of variance. When the data conformed to the normal distribution, the two groups of data were tested using the independent sample t test, and the three or more groups of data were subjected to one-way ANOVA analysis of variance, expressed as the mean ± standard deviation of three independent repeated data samples. For statistical differences between groups, the LSD test was used if the variance was not equal. If the variance was not equal, the data were tested using Dunnett's T3. P < 0.05 was considered statistically significant. When the data did not conform to the normal distribution, a nonparametric test was used to analyze the data, and the data were expressed as M (P25-P75). GraphPad Prism 8.0 software was used to prepare all data graphs and calculate IC50. The smaller the IC50 value, the stronger the inhibitory ability against tyrosinase. Calculation of tyrosinase inhibition rate: [1-(CD) / (AB)] × 100%

[0118] Where:

[0119] C—absorbance value of the sample tube, i.e., absorbance value of the solution after the sample reacts with tyrosinase;

[0120] D—sample background absorbance;

[0121] A—absorbance of enzyme reaction tube, i.e. absorbance of tyrosinase and DOPA reaction without adding sample;

[0122] B—Solvent background absorbance;

[0123] The test results are shown in Table 9 below: Tyrosinase inhibition rate

[0124] Table 9

[0125]

[0126] It can be seen from the above table that the tyrosinase inhibition rate of the whitening sunscreen composition prepared in the embodiment of the present invention is above 80%.

[0127] Performance Test-5 Repair Barrier Test

[0128] Filaggrin (FLG): It is a water-soluble acid / neutral protein present in human epidermal keratinocytes (KC). It is easily soluble in water and is a matrix protein between the filaments of the eukaryotic cytoskeleton cells. It participates in the formation of the intercellular corneum cell capsule (CE) during the terminal differentiation of KC. Its precursor is Profilaggrin, which becomes Filaggrin after being dephosphorylated by Caspase-14, and then hydrolyzed into various natural skin barrier factors, which maintain the water content of the stratum corneum, participate in skin hydration, and maintain the skin barrier. Filaggrin can also initiate the apoptosis of KC, thereby transforming KC from granular layer cells with nuclei to corneous cells without nuclei. When keratinocytes are damaged, the expression of the filaggrin gene decreases, and the decrease in the content of filaggrin causes damage to the skin barrier. This patent stimulates keratinocytes through UV to simulate skin damage. The skin barrier is characterized by comparing the expression of the FLG gene. The repair ability of the sample is judged by comparing the expression of the FLG gene before and after the use of the sample.

[0129] Specifically, HaCaT cells were plated on a 6-well plate, and the culture medium was discarded after culturing for 24 h. The cells were washed with PBS. The blank group was not irradiated with UVB, and the model group (irradiated with UVB) and the experimental group (irradiated with UVB + tested samples) were exposed to 80 mJ / cm 2 The cells were irradiated with UVB, and then complete culture medium containing different concentrations of the tested samples was added. After 48 hours, total RNA was extracted using TRIzon, and RNA purity and concentration were tested using NanoDrop. Subsequently, cDNA was synthesized and amplified by PCR, and the target gene band was confirmed by agarose gel electrophoresis analysis. Finally, cDNA was used for Real-Time PCR quantitative detection to obtain the effect of the tested samples on the expression of genes related to the skin barrier of UV-damaged HaCaT cells.

[0130] The experimental results are as follows: The expression levels of FLG gene in different samples are shown in Table 10.

[0131] Table 10

[0132] project FLG expression project FLG expression project FLG expression Blank Group 100±7.46 Example 9 117.00±4.49* Comparative Example 7 70.2±12.45 Model Group 65.67±4.31### Example 10 151.33±13.33* Comparative Example 8 69.8±5.12 Example 1 185.6±23.50*** Embodiment 11 126.00±6.93* Comparative Example 9 69.08±6.97 Example 2 175.33±13.81*** Example 12 121.33±13.33* Comparative Example 10 73.12±17.21 Example 3 177.67±11.63*** Comparative Example 1 82.33±4.58* - - Example 4 160.33±10.40*** Comparative Example 2 82.33±4.58* - - Example 5 120.33±11.26*** Comparative Example 3 95.2±4.03* - - Example 6 119.33±2.54*** Comparative Example 4 85.4±7.24* - - Example 7 138.67±9.99** Comparative Example 5 71.67±6.20 - - Example 8 134.33±11.03** Comparative Example 6 67.33±0.27 - -

[0133] Note: ### represents p<0.001 between the model group and the blank group, * represents p<0.05 between the embodiment and the model group, ** represents p<0.01 between the embodiment and the model group, and *** represents p<0.001 between the embodiment and the model group.

[0134] It can be seen from the above table that the whitening sunscreen composition prepared in the embodiment of the present invention has an FLG gene expression level of more than 100, and has a good skin barrier repair effect.

[0135] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A plant whitening and sunscreen composition, characterized in that: The invention comprises the following components in parts by weight: 0.5-5 parts of a P / O / W composite carrier, 0.1-3 parts of bakuchiol, and 0.1-3 parts of a thyme extract; the P / O / W composite carrier comprises three phases in the following mass percentages: 8-20% of an alcohol phase, 20-35% of an oil phase, and 50-70% of an aqueous phase; the alcohol phase comprises verbascoside, pterostilbene, and a polyol; the oil phase comprises an emulsifier and oil; and the aqueous phase comprises solid particles and deionized water; wherein The polyol comprises at least two of butylene glycol, glycerol, dipropylene glycol, propylene glycol, and PEG400; the oil comprises at least one of butyl octanol salicylate, C12-15 alcohol benzoate, isononyl isononanoate, and dicaprylyl carbonate; the solid particles are at least one of cellulose and modified starch; in the P / O / W composite carrier, the mass percentage of verbascoside is 0.1-2%, and the mass percentage of pterostilbene is 0.1-5%.

2. The plant whitening sunscreen composition according to claim 1, characterized in that: The weight ratio of the P / O / W composite carrier, bakuchiol and thyme extract is P / O / W composite carrier: bakuchiol: thyme extract = 1: (0.1-0.8): (0.1-0.8).

3. The plant whitening sunscreen composition according to claim 1, characterized in that: In the plant whitening and sunscreen composition, the mass percentage of the P / O / W composite carrier is 45-60%.

4. The plant whitening sunscreen composition according to claim 1, characterized in that: The polyol is a mixture of glycerol and dipropylene glycol, and the weight ratio of the glycerol to dipropylene glycol is (2-8): (2-8).

5. The plant whitening sunscreen composition according to claim 1, characterized in that: The polyol is a mixture of glycerol and dipropylene glycol, and the weight ratio of the glycerol to dipropylene glycol is 1:

1.

6. The plant whitening sunscreen composition according to claim 1, characterized in that: The preparation method of the P / O / W composite carrier comprises the following steps: S1, dissolving verbascoside and pterostilbene in polyol to prepare an inner alcohol phase; S2, adding an emulsifier to the oil to obtain an oil phase; S3, adding the inner alcohol phase to the oil phase, and homogenizing and dispersing to obtain P / O colostrum; S4, adding solid particles into deionized water to disperse and obtain an aqueous phase; S5. Add the P / O colostrum into the aqueous phase and disperse it homogeneously to obtain a P / O / W composite carrier.

7. The plant whitening sunscreen composition according to claim 6, characterized in that: In the step S1, the weight ratio of verbascoside, pterostilbene and polyol is verbascoside: pterostilbene: polyol = (0.1-2): (0.1-5): 10; And / or, in step S2, the weight ratio of oil to emulsifier is oil: emulsifier = (1-10): 1; And / or, in step S3, the weight ratio of the inner alcohol phase to the oil phase is inner alcohol phase: oil phase = (2-4): (6-8); And / or, in step S4, the weight ratio of solid particles to deionized water is solid particles: deionized water = 1: (10-30); And / or, in step S5, the weight ratio of P / O colostrum to aqueous phase is P / O colostrum: aqueous phase = (2-5): (5-8).

8. Use of the plant whitening and sunscreen composition according to any one of claims 1 to 7 in the preparation of skin products.

9. A skin care product, characterized in that: The skin products include the plant whitening sunscreen composition according to any one of claims 1 to 7; the skin products include one of sunscreen milk, sunscreen lotion, sunscreen cream, sunscreen liquid, sunscreen powder, sunscreen spray, sunscreen gel, isolation cream, isolation milk, isolation lotion, and BB cream.

10. The skin care product according to claim 9, wherein: The skin product comprises the following components in percentage by mass: 0.03-13% of the plant whitening sunscreen composition according to any one of claims 1 to 7, 2-30% of a chemical sunscreen and / or an inorganic ultraviolet blocker, 5-40% of a cosmetic base, and the balance being water; the cosmetic base comprises at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster.

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