Repair composition for removing striae gravidarum as well as preparation method and application thereof

Abstract

The invention relates to a repair composition for removing striae gravidarum as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition comprises the following components: Inca inchi seed oil, a Chinese holly extract, a grape seed extract, an areca flower extract, alligator oil, vitamin E and polydeoxyribonucleotide. All the components are coordinated to promote the proliferation of skin fibroblasts, so that the stretch marks can be reduced and faded. According to the preparation method, the polydeoxynucleotide in the composition is prepared into the nano-liposome, so that the transdermal performance of the composition is enhanced, the stability of the composition is effectively improved, the polydeoxynucleotide is promoted to be slowly released and stored in a skin care target part, and the bioavailability of functional components of cosmetics is effectively improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular, relates to a repairing composition for removing stretch marks, a preparation method and an application thereof. Background Art

[0002] Stretch marks (Striae gravidarum, SG) are one of the most common connective tissue changes during pregnancy, and a large number of pregnant women are troubled by stretch marks. Early stretch marks often appear as flat, pink or red stripes, which gradually protrude from the skin surface and become longer and wider purple-red stripes (red stripes or immature stripes). Over a period of months to years, the skin lesions gradually atrophy and are accompanied by hypopigmentation and loss, forming scar-like atrophic lines (white stripes or mature stripes) parallel to the skin tension lines. The skin lesions are prone to occur on the breasts, abdomen, buttocks and thighs. Occasionally, there are subjective symptoms such as itching and burning pain. Although stretch marks do not threaten the patient's physical health, they can cause psychological diseases because they affect the beauty of the skin. Summary of the invention

[0003] The purpose of the present invention is to provide a repairing composition for removing stretch marks, which can effectively remove stretch marks.

[0004] The second object of the present invention is to provide a method for preparing the above composition.

[0005] The third object of the present invention is to provide application of the above composition.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A repair composition for removing stretch marks, comprising the following components in parts by weight:

[0008]

[0009]

[0010] As a preferred embodiment of the present invention, the composition further comprises 1 to 10 parts by weight of a moisturizing component, wherein the moisturizing component is at least one of squalane and polyethylene glycol.

[0011] As a preferred embodiment of the present invention, the composition further comprises 0.1 to 1 parts by weight of a thickener, wherein the thickener is at least one of starch, carbomer, collagen and sodium alginate.

[0012] As a preferred embodiment of the present invention, the Sijiqing extract is extracted with an ethanol aqueous solution with a mass concentration of 70-90%, the extraction temperature is 60° C., and the solid-liquid ratio is 1:10-20.

[0013] As a preferred embodiment of the present invention, the grape seed extract is extracted with an ethanol aqueous solution with a mass concentration of 50-70%, and the solid-liquid ratio is 1:15-30.

[0014] As a preferred embodiment of the present invention, the betel nut flower extract is extracted with an ethanol aqueous solution with a mass concentration of 60-80%, and the solid-liquid ratio is 1:10-30.

[0015] As a preferred embodiment of the present invention, the polydeoxyribonucleotide is replaced by a nanolipid coating, and the preparation method of the nanolipid coating comprises the following steps:

[0016] Quantitatively weigh lecithin and cholesterol, mix with anhydrous ethanol to obtain phase A, mix polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water, dissolve at 60-70° C. to obtain phase B, mix phase A and phase B and homogenize them under high pressure to obtain a nanolipid coating.

[0017] As a preferred embodiment of the present invention, the usage ratio of lecithin, cholesterol, anhydrous ethanol, polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water is 5-6g: 0.5-1g: 5-10mL: 1g: 1.5-2g: 0.5-1g: 10-20mL.

[0018] The preparation method of the above composition comprises the following steps:

[0019] Heat water accounting for 60-70% of the total weight of the components of the composition to 60-70° C., disperse it evenly, and then add the evergreen extract, grape seed extract, and betel nut flower extract and mix them evenly to obtain a mixture A;

[0020] Add polydeoxyribonucleotide into water to prepare a suspension, and then mix the suspension with mixture A to obtain mixture B;

[0021] Mix Inca Inchi Seed Oil, Crocodile Oil and Vitamin E to make mixture C;

[0022] The mixture B and the mixture C are mixed, emulsified, cooled to room temperature, and homogenized to prepare a composition.

[0023] The above composition is used as an effective ingredient in the preparation of a product for removing stretch marks.

[0024] Beneficial effects of the present invention:

[0025] 1. The composition of the present invention comprises sacha inchi seed oil, iris extract, grape seed extract, betel nut flower extract, crocodile oil, vitamin E, and polydeoxyribonucleotides. Among them, sacha inchi seed oil is rich in various unsaturated fatty acids such as Omega-3, Omega-6, and Omega-9, which help to improve the elasticity and toughness of the skin. In addition, sacha inchi seed oil promotes the regeneration and repair of skin cells and enhances the elasticity of skin tissue, thereby helping to reduce and fade stretch marks.

[0026] Evergreen extract can promote the proliferation and migration of skin fibroblasts. Abundant fibroblasts can produce the greatest contraction force, help reduce and fade stretch marks, and have excellent anti-inflammatory ability. Grape seed extract and areca nut flower extract are rich in epicatechin and gallic acid. The synergistic effect of the three can protect fibroblasts by clearing intracellular reactive oxygen and inhibiting mitochondrial membrane damage, helping to reduce and fade stretch marks.

[0027] Crocodile oil is rich in various active ingredients, such as C20:5 (EPA) arachidonic acid, linolenic acid, etc. These ingredients help to repair and regenerate stretch marks skin. EPA in crocodile oil can effectively induce human epidermal keratinocyte proliferation and fibroblast differentiation, promote skin repair, arachidonic acid has the function of repairing skin damage and increasing the elasticity of connective tissue; linolenic acid gives cell membranes fluidity, flexibility and selective permeability. Therefore, crocodile oil can remove stretch marks by promoting skin cell regeneration and repairing broken fibrous tissue.

[0028] Vitamin E can protect the skin from damage by inhibiting the production of free radicals and stabilizing cell membranes. The antioxidant effect of vitamin E helps reduce inflammatory responses and promote collagen synthesis, thereby reducing the appearance and deepening of stretch marks. For stretch marks that have already formed, vitamin E also has a certain auxiliary lightening effect.

[0029] Polydeoxyribonucleotides are a substance that is highly homologous to human DNA and has the special ability to promote skin recovery and reorganization. In terms of removing stretch marks, polydeoxyribonucleotides penetrate into the sunken areas through transdermal administration, reactivate the proliferation and growth patterns of normal cells, and promote the growth and repair of skin cells.

[0030] Further, in the composition of the present invention, vitamin E enhances the stability of crocodile oil and inca inchi seed oil at the mass fraction of the present invention. Vitamin E can prevent the unsaturated fatty acids in inca inchi seed oil from being attacked by free radicals and undergoing oxidation reaction, and vitamin E can effectively slow down the oxidation rate of crocodile oil and enhance the stability of the resulting composition.

[0031] 2. In the preparation method of the present invention, the polydeoxynucleotide in the composition is made into nanoliposomes, which enhances the transdermal performance of the composition, effectively improves the stability of the composition, promotes the sustained release of the polydeoxynucleotide and its retention in the target skin care parts, and effectively improves the bioavailability of the cosmetic functional ingredients. DETAILED DESCRIPTION

[0032] The present invention is further described in detail below in conjunction with specific implementation methods.

[0033] Inca inchi seed oil was purchased from Greentech, France, batch number: II210807-AI-D-BIO; crocodile oil was purchased from Fujian Lulong Industrial Co., Ltd.; polydeoxyribonucleotides were purchased from Runhui Biotechnology (Weihai) Co., Ltd., which will not be repeated below.

[0034] Example 1

[0035] The polydeoxyribonucleotides are made into nanolipid coatings, and the specific operation is as follows:

[0036] Quantitatively weigh lecithin and cholesterol, mix with anhydrous ethanol to obtain phase A, mix polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water, dissolve at 60°C to obtain phase B, mix phase A and phase B, stir for 30 minutes and then homogenize under high pressure to obtain 5 kg of nanolipid encapsulated bodies. Among them, the dosage ratio of lecithin, cholesterol, anhydrous ethanol, polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water is 5g:0.5g:5mL:1g:1.5g:0.5g:10mL.

[0037] Preparation of Evergreen extract: extraction with 70% ethanol aqueous solution for 2 h, extraction temperature of 60° C., and solid-liquid ratio of 1:10.

[0038] Preparation of grape seed extract: Extract with 50% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:15.

[0039] Preparation of Areca Flower Extract: Extract with 60% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:10.

[0040] The preparation of the repair composition for removing stretch marks is carried out as follows:

[0041] Heat water accounting for 60 wt% of the total weight of the components of the composition after addition to 60° C., disperse evenly, and then add the evergreen extract, grape seed extract, and betel nut flower extract and mix evenly to obtain a mixture A;

[0042] Adding the nanolipid inclusion body made of polydeoxyribonucleotide into water to prepare a suspension, and then mixing the suspension with mixture A to obtain mixture B;

[0043] Mix the inca inchi seed oil, crocodile oil, vitamin E and moisturizing components to obtain a mixture C;

[0044] Mixture B and mixture C were mixed and emulsified, and then a thickener was added and mixed evenly. The mixture was cooled to room temperature and homogenized with a high-pressure homogenizer to obtain 10 kg of a composition.

[0045] Example 2

[0046] The polydeoxyribonucleotides are made into nanolipid coatings, and the specific operation is as follows:

[0047] Quantitatively weigh lecithin and cholesterol, mix with anhydrous ethanol to obtain phase A, mix polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water, dissolve at 62°C to obtain phase B, mix phase A and phase B, stir for 30 minutes and then homogenize under high pressure to obtain 5 kg of nanolipid encapsulated bodies. Among them, the dosage ratio of lecithin, cholesterol, anhydrous ethanol, polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water is 5.2 g: 0.6 g: 6 mL: 1 g: 1.6 g: 0.6 g: 12 mL.

[0048] Preparation of Evergreen extract: extraction with 75% ethanol aqueous solution for 2 h, extraction temperature of 60° C., and solid-liquid ratio of 1:12.

[0049] Preparation of grape seed extract: Extract with 55% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:18.

[0050] Preparation of Areca Flower Extract: Extract with 65% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:15.

[0051] The preparation of the repair composition for removing stretch marks is carried out as follows:

[0052] Heat water accounting for 62 wt% of the total weight of the components of the composition after addition to 62° C., disperse it evenly, then add the evergreen extract, grape seed extract, and betel nut flower extract and mix evenly to obtain a mixture A;

[0053] Adding the nanolipid inclusion body made of polydeoxyribonucleotide into water to prepare a suspension, and then mixing the suspension with mixture A to obtain mixture B;

[0054] Mix the inca inchi seed oil, crocodile oil, vitamin E and moisturizing components to obtain a mixture C;

[0055] Mixture B and mixture C were mixed and emulsified, and then a thickener was added and mixed evenly. The mixture was cooled to room temperature and homogenized with a high-pressure homogenizer to obtain 10 kg of a composition.

[0056] Example 3

[0057] The polydeoxyribonucleotides are made into nanolipid coatings, and the specific operation is as follows:

[0058] Quantitatively weigh lecithin and cholesterol, mix with anhydrous ethanol to obtain phase A, mix polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water, dissolve at 65°C to obtain phase B, mix phase A and phase B, stir for 30 minutes and then homogenize under high pressure to obtain 5 kg of nano lipid encapsulated body. Among them, the dosage ratio of lecithin, cholesterol, anhydrous ethanol, polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water is 5.5 g: 0.8 g: 8 mL: 1 g: 1.8 g: 0.8 g: 15 mL.

[0059] Preparation of Evergreen extract: extraction with 80% ethanol aqueous solution for 2 h, extraction temperature of 60° C., solid-liquid ratio of 1:15.

[0060] Preparation of grape seed extract: Extract with 60% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:22.

[0061] Preparation of Areca Flower Extract: Extract with 70% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:20.

[0062] The preparation of the repair composition for removing stretch marks is carried out as follows:

[0063] Heat water accounting for 65 wt% of the total weight of the components of the composition after addition to 65° C., disperse it evenly, and then add the evergreen extract, grape seed extract, and betel nut flower extract and mix them evenly to prepare a mixture A;

[0064] Adding the nanolipid inclusion body made of polydeoxyribonucleotide into water to prepare a suspension, and then mixing the suspension with mixture A to obtain mixture B;

[0065] Mix the inca inchi seed oil, crocodile oil, vitamin E and moisturizing components to obtain a mixture C;

[0066] Mixture B and mixture C were mixed and emulsified, and then a thickener was added and mixed evenly. The mixture was cooled to room temperature and homogenized with a high-pressure homogenizer to obtain 10 kg of a composition.

[0067] Example 4

[0068] The polydeoxyribonucleotides are made into nanolipid coatings, and the specific operation is as follows:

[0069] Quantitatively weigh lecithin and cholesterol, mix with anhydrous ethanol to obtain phase A, mix polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water, dissolve at 68°C to obtain phase B, mix phase A and phase B, stir for 30 minutes and then homogenize under high pressure to obtain 5 kg of nano lipid encapsulated body. Among them, the amount ratio of lecithin, cholesterol, anhydrous ethanol, polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water is 5.8g:0.9g:9mL:1g:1.9g:0.9g:18mL.

[0070] Preparation of Evergreen extract: extraction with 85% ethanol aqueous solution for 2 h, extraction temperature of 60° C., and solid-liquid ratio of 1:18.

[0071] Preparation of grape seed extract: Extract with 65% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:27.

[0072] Preparation of Areca Flower Extract: Extract with 75% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:25.

[0073] The preparation of the repair composition for removing stretch marks is carried out as follows:

[0074] Heat water accounting for 68 wt% of the total weight of the components of the composition after addition to 68° C., disperse evenly, and then add the evergreen extract, grape seed extract, and betel nut flower extract and mix evenly to obtain a mixture A;

[0075] Adding the nanolipid inclusion body made of polydeoxyribonucleotide into water to prepare a suspension, and then mixing the suspension with mixture A to obtain mixture B;

[0076] Mix the inca inchi seed oil, crocodile oil, vitamin E and moisturizing components to obtain a mixture C;

[0077] Mixture B and mixture C were mixed and emulsified, and then a thickener was added and mixed evenly. The mixture was cooled to room temperature and homogenized with a high-pressure homogenizer to obtain 10 kg of a composition.

[0078] Example 5

[0079] The polydeoxyribonucleotides are made into nanolipid coatings, and the specific operation is as follows:

[0080] Quantitatively weigh lecithin and cholesterol, mix with anhydrous ethanol to obtain phase A, mix polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water, dissolve at 70°C to obtain phase B, mix phase A and phase B, stir for 30 minutes and then homogenize under high pressure to obtain 5 kg of nano-lipid encapsulated bodies. Among them, the usage ratio of lecithin, cholesterol, anhydrous ethanol, polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water is 6g: 1g: 10mL: 1g: 2g: 1g: 20mL.

[0081] Preparation of Evergreen extract: extraction with 90% ethanol aqueous solution for 2 h, extraction temperature of 60° C., and solid-liquid ratio of 1:20.

[0082] Preparation of grape seed extract: Extract with 70% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:30.

[0083] Preparation of Areca Flower Extract: Extract with 80% ethanol aqueous solution for 2 h, with a solid-liquid ratio of 1:30.

[0084] The preparation of the repair composition for removing stretch marks is carried out as follows:

[0085] Heat water accounting for 70 wt% of the total weight of the components of the composition after addition to 70° C., disperse it evenly, and then add the evergreen extract, grape seed extract, and betel nut flower extract and mix them evenly to prepare a mixture A;

[0086] Adding the nanolipid inclusion body made of polydeoxyribonucleotide into water to prepare a suspension, and then mixing the suspension with mixture A to obtain mixture B;

[0087] Mix the inca inchi seed oil, crocodile oil, vitamin E and moisturizing components to obtain a mixture C;

[0088] Mixture B and mixture C were mixed and emulsified, and then a thickener was added and mixed evenly. The mixture was cooled to room temperature and homogenized with a high-pressure homogenizer to obtain 10 kg of a composition.

[0089] The weight parts of each component in Examples 1 to 5 are shown in Table 1:

[0090] Table 1

[0091]

[0092] Based on Example 3, the weight parts of the components were adjusted, and the rest were the same as in Example 3 to obtain a composition, and the rest were the same as in Example 3. The specific weight parts are shown in Table 2:

[0093] Table 2

[0094]

[0095]

[0096] Comparative Example 7

[0097] Compared with Example 3, polydeoxyribonucleotides are used instead of polydeoxyribonucleotides (nano-lipid coatings), that is, polydeoxyribonucleotides are not made into nano-lipid coatings, and the rest are the same as Example 3.

[0098] The compositions prepared in Examples 1 to 5 and Comparative Examples 1 to 7 were tested as follows:

[0099] Test Example 1 Cell proliferation experiment

[0100] Fibroblasts in the logarithmic growth phase were collected and counted at 4 × 10 3 The cells were inoculated in a 96-well plate at a density of 100 μL per well, and cultured at 5% CO2 and 37°C for 24 hours. The cells were divided into a blank control group and a drug-treated group. The blank control group was added with 100 μL of DMEM complete medium, and the drug-treated group was added with 100 μL of the composition prepared in Examples 1 to 5 and Comparative Examples 1 to 7 containing 50 μg / mL, with 3 replicates per group. The culture was continued for 48 hours, 10 μL of CCK-8 solution was added to each well, and the culture was continued for 2 hours. The absorbance of each well was then measured at a wavelength of 450 nm using an ELISA reader, and the survival rate of the fibroblasts was calculated. The results are shown in Table 3.

[0101] Table 3

[0102] Group Survival rate of fibroblasts / % Blank Group 100.0 Example 1 106.5 Example 2 109.7 Example 3 112.4 Example 4 110.1 Example 5 108.0 Comparative Example 1 101.3 Comparative Example 2 102.2 Comparative Example 3 103.7 Comparative Example 4 103.9 Comparative Example 5 102.2 Comparative Example 6 103.4 Comparative Example 7 104.3

[0103] As shown in Table 3, the survival rates of fibroblasts in the compositions prepared in Examples 1 to 5 are all greater than 105% in the cell proliferation experiment test, which shows that the compositions prepared in Examples 1 to 5 can promote cell proliferation. Comparative Examples 1 to 6 replace the components of Sacha Inchi seed oil, Evergreen extract, grape seed extract, Areca nut flower extract, crocodile oil, and vitamin E, respectively, but the survival rates of fibroblasts are all less than 105%, which shows that the components Sacha Inchi seed oil, Evergreen extract, grape seed extract, Areca nut flower extract, crocodile oil, and vitamin E have a synergistic effect on the survival rate of fibroblasts. In Comparative Example 7, the survival rate of fibroblasts is 104.3%, because the polydeoxyribonucleotides are not made into nano-lipid coatings in Comparative Example 7, so the large particle size of the polydeoxyribonucleotides affects the survival rate of fibroblasts.

[0104] Test Example 2 Stability Test

[0105] The compositions obtained in Examples 1 to 5 and Comparative Example 6 were oxidized in a 60° C. thermostat for 10 days and then the peroxide values ​​of each group were measured by the Schaal method before and after oxidation. The results are shown in Table 4.

[0106] Table 4

[0107] Group 0d peroxide value (mmol / kg) 10d peroxide value (mmol / kg) Example 1 0.13 5.34 Example 2 0.06 5.57 Example 3 0.11 5.49 Example 4 0.02 5.16 Example 5 0.07 5.69 Comparative Example 6 0.09 23.12

[0108] As shown in Table 4, the peroxide values ​​of the compositions prepared in Comparative Examples 1 to 5 were all less than 6 mmol / kg at 10 days, which was much lower than the peroxide value of 23.12 mmol / kg at 10 days in Comparative Example 6. It can be seen that the presence of vitamin E in the components significantly reduced the peroxide value of the composition and enhanced the stability of crocodile oil and inca inchi seed oil in the composition.

[0109] The above is only an implementation method of the invention, and does not limit the scope of patent protection. Those skilled in the art can make non-substantial changes or substitutions based on the invention, which still fall within the scope of patent protection.

Claims

1. A repairing composition for removing stretch marks, characterized in that: Contains the following components by weight: 10-20 parts of Inca Inchi Seed Oil 1~5 parts of Sijiqing extract Grape seed extract 1~10 parts Areca nut flower extract 0.1~3 parts 5~15 parts crocodile oil Vitamin E 1~5 parts 0.1~2 parts of polydeoxyribonucleotides.

2. The composition according to claim 1, characterized in that The composition also contains 1 to 10 parts by weight of a moisturizing component, wherein the moisturizing component is at least one of squalane and polyethylene glycol.

3. The composition according to claim 1, characterized in that The composition also contains 0.1 to 1 parts by weight of a thickener, wherein the thickener is at least one of starch, carbomer, collagen and sodium alginate.

4. The composition according to claim 1, characterized in that The Sijiqing extract is extracted with an ethanol aqueous solution with a mass concentration of 70-90%, an extraction temperature of 60°C, and a solid-liquid ratio of 1:10-20.

5. The composition according to claim 1, characterized in that The grape seed extract is extracted with an ethanol aqueous solution having a mass concentration of 50-70% and a solid-liquid ratio of 1:15-30.

6. The composition according to claim 1, characterized in that The betel nut flower extract is extracted with an ethanol aqueous solution with a mass concentration of 60-80%, and the solid-liquid ratio is 1:10-30.

7. The composition according to claim 1, characterized in that The polydeoxyribonucleotide is replaced by a nanolipid coating, and the preparation method of the nanolipid coating comprises the following steps: Quantitatively weigh lecithin and cholesterol, mix with anhydrous ethanol to obtain phase A, mix polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water, dissolve at 60-70°C to obtain phase B, mix phase A and phase B and homogenize them under high pressure to obtain nanolipid encapsulation.

8. The composition according to claim 7, characterized in that The dosage ratio of the lecithin, cholesterol, anhydrous ethanol, polydeoxyribonucleotide, PEG-40 hydrogenated castor oil, glycerol and water is 5-6g: 0.5-1g: 5-10mL: 1g: 1.5-2g: 0.5-1g: 10-20mL.

9. A method for preparing the composition according to any one of claims 1 to 8, characterized in that: Heat water accounting for 60-70% of the total weight of the components of the composition after addition to 60-70° C., slowly add vitamin E and disperse it evenly, then add the evergreen extract, grape seed extract, and betel nut flower extract and mix them evenly to obtain a mixture A; Adding polydeoxyribonucleotide into water to prepare a suspension, and then mixing the suspension with mixture A to obtain mixture B; Mix Inca Inchi Seed Oil, Crocodile Oil and Vitamin E to make Mixture C; The mixture B and the mixture C are mixed, emulsified, cooled to room temperature, and homogenized to prepare a composition.

10. Use of the composition according to any one of claims 1 to 8 as an effective ingredient in the preparation of a product for removing stretch marks.

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