Quantitative prediction method and system for endogenous volatile organic compounds in expired gas of human body

Abstract

The invention relates to a quantitative prediction system for endogenous volatile organic compounds in exhaled gas of a human body, and the system comprises an exhaled gas collection device which is used for obtaining the exhaled gas of a target human body; the input end of the flow-through type medium channel discharge source is directly connected with the output end of the exhaled gas collecting device, and the flow-through type medium channel discharge source is used for receiving the exhaled gas of the target human body and acting on the exhaled gas of the target human body to output soft ionization gaseous molecules; the input end of the GC-MS equipment is directly connected with the output end of the flow-through type medium path discharge source, and the GC-MS equipment is used for ionizing the soft ionization gaseous molecules and analyzing the ionized soft ionization gaseous molecules through a carbon dioxide isotope internal standard quantitative method to obtain a prediction result; and the PC equipment is in communication connection with the GC-MS equipment and is used for acquiring the prediction result and visually displaying the prediction result. According to the system provided by the invention, the whole quantitative prediction process of the human body exhaled gas is high in real-time performance and easy to operate, and the components of the human body exhaled gas can be rapidly and conveniently predicted.

Description

Technical Field

[0001] The present invention relates to the technical field related to gas composition prediction, and in particular to a quantitative prediction system for endogenous volatile organic compounds in human exhaled breath. Background Art

[0002] Endogenous compounds in human exhaled breath, including inorganic gases (such as NO and CO) and VOCs (such as isoprene, ethane, pentane, acetone), are metabolites of human tissues. By analyzing the components and predicting the concentrations of endogenous markers, relevant information on human tissue metabolism and health status can be obtained non-invasively, which helps to clarify the corresponding physiological processes, disease occurrence, disease progression, and pharmacological responses.

[0003] The main disadvantages of the current breath diagnosis technology based on gas chromatography are the lack of dynamics and difficulty in quantification. Breathing is a dynamic process, and the analytical methods currently reported are mostly static, which makes quantitative determination difficult. The quantitative method and accurate determination of endogenous markers need further research. Summary of the invention

[0004] The purpose of the present invention is to solve at least one of the deficiencies of the prior art and to provide a quantitative prediction system for endogenous volatile organic compounds in human exhaled breath.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: Specifically, a quantitative prediction system for endogenous volatile organic compounds in human exhaled breath is proposed, including the following: An exhaled gas collection device, used to obtain exhaled gas from a target human body; A flow-through medium channel discharge source, the input end of which is directly connected to the output end of the exhaled gas collection device, for receiving the exhaled gas of the target human body and acting on the exhaled gas of the target human body to output soft ionized gaseous molecules; A GC-MS device, the input end of which is directly connected to the output end of the flow-through medium passage discharge source, is used to ionize the soft ionized gaseous molecules, and analyze the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a prediction result; The PC device is connected to the GC-MS device for obtaining the prediction results and visually displaying the prediction results.

[0006] Further, specifically, the exhaled gas collection device includes an oral exhaled air collection module and a nasal exhaled air collection module, wherein: When sampling with the mouth-expiratory collection module, you need to pinch your nose and blow air into a disposable straw only with your mouth. When sampling with the nasal-expiratory collection module, you need to hold a fixing device in your mouth, place the head of the disposable straw on the outside of your nostrils, and exhale alternately through both nostrils.

[0007] Further, specifically, the flow-through medium passage discharge source is integrated with the GC-MS device, that is, the flow-through medium passage discharge source is connected in series with the GC-MS device and integrated in the same device housing.

[0008] Furthermore, the exhaled gas collection device further comprises: A disposable filter head is arranged at the front end of the disposable straw used for sampling by the oral exhalation collection module and the nasal exhalation collection module, and the disposable filter head is used to filter out suspended particles and water vapor in human exhaled air.

[0009] Further, specifically, the ionized soft ionized gaseous molecules are analyzed by carbon dioxide isotope internal standard quantitative method to obtain prediction results, including: Determine the types of endogenous markers and the normal value range of each endogenous marker based on samples from a healthy population through preliminary experiments; Performing an endogenous marker quantitative analysis on the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a content value of a predetermined type of endogenous marker in the exhaled gas of a target human body; Determine whether the content value of any type of endogenous marker obtained by quantitative analysis is within its corresponding normal value range. If so, mark it as normal; if not and above the normal value range, mark it as abnormally high; if not and below the normal value range, mark it as abnormally low.

[0010] Further, specifically, the ionized soft ionized gaseous molecules are subjected to endogenous marker quantitative analysis by carbon dioxide isotope internal standard quantitative method to obtain the content value of the predetermined endogenous marker type in the target human exhaled gas, including: The carbon dioxide isotope peak used for quantification of endogenous markers is determined by preliminary experiments; By comparing the input data after ionization with the abundance of each endogenous marker, the isotope peak with a peak intensity close to that of the endogenous marker in exhaled breath was selected as the reference standard, and the relative ratios of different markers to the carbon dioxide isotope peak were analyzed for quantification.

[0011] Further, specifically, the PC terminal visually displays the prediction results, including: The name of each endogenous marker, the corresponding content value and the abnormal analysis situation are associated and displayed visually in the form of a chart.

[0012] Furthermore, when debugging instrument parameters, the GC-MS device includes: Through preliminary experiments, known endogenous volatile organic compounds commonly found in human exhaled breath are used as references for testing and analysis, and instrument parameters are debugged, including sensitivity.

[0013] The present invention also provides a quantitative prediction method for endogenous volatile organic compounds in human exhaled breath, which is applied to the quantitative prediction system for endogenous volatile organic compounds in human exhaled breath, comprising: Acquire the exhaled gas of the target human body through an exhaled gas collection device; The discharge source receives the exhaled gas of the target human body through the flow-through medium channel, and acts on the exhaled gas of the target human body to output soft ionized gaseous molecules; Ionizing the soft ionized gaseous molecules by a GC-MS device, and analyzing the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a prediction result; The prediction results are obtained through a PC device and visualized.

[0014] The beneficial effects of the present invention are: The present invention proposes a quantitative prediction system for endogenous volatile organic compounds in human exhaled breath, which quickly obtains human exhaled gas through an exhaled gas collection device, and then predicts the results of the obtained target human exhaled gas based on a carbon dioxide isotope internal standard quantitative method through a series of flow-through medium passage discharge sources and GC-MS equipment, and finally performs visual display and data analysis through a PC. The whole process is highly real-time and easy to operate, and can quickly and conveniently predict the components of human exhaled gas. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The above and other features of the present disclosure will become more apparent by describing in detail the embodiments shown in the accompanying drawings. The same reference numerals in the accompanying drawings of the present disclosure represent the same or similar elements. Obviously, the accompanying drawings described below are only some embodiments of the present disclosure. For those skilled in the art, other accompanying drawings can be obtained based on these accompanying drawings without creative work. In the accompanying drawings: Figure 1 Shown is a structural principle diagram of a quantitative prediction system for endogenous volatile organic compounds in human exhaled breath of the present invention. DETAILED DESCRIPTION

[0016] The following will be combined with the embodiments and drawings to clearly and completely describe the concept, specific structure and technical effects of the present invention, so as to fully understand the purpose, scheme and effect of the present invention. It should be noted that the embodiments in this application and the features in the embodiments can be combined with each other without conflict. The same reference numerals used throughout the drawings indicate the same or similar parts.

[0017] Example 1, reference Figure 1 The present invention proposes a quantitative prediction system for endogenous volatile organic compounds in human exhaled breath, comprising the following: An exhaled gas collection device, used to obtain exhaled gas from a target human body; A flow-through medium channel discharge source, the input end of which is directly connected to the output end of the exhaled gas collection device, for receiving the exhaled gas of the target human body and acting on the exhaled gas of the target human body to output soft ionized gaseous molecules; A GC-MS device, the input end of which is directly connected to the output end of the flow-through medium passage discharge source, is used to ionize the soft ionized gaseous molecules, and analyze the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a prediction result; The PC device is connected to the GC-MS device for obtaining the prediction results and visually displaying the prediction results.

[0018] In this embodiment 1, human exhaled gas is quickly acquired through an exhaled gas collection device, and then the acquired target human exhaled gas is passed through a series of flow-through medium passage discharge sources and a GC-MS device to perform result prediction based on a carbon dioxide isotope internal standard quantitative method, and finally a visual display and data analysis are performed through a PC. The whole process is highly real-time and easy to operate, and can quickly and conveniently predict the composition of human exhaled gas.

[0019] As a preferred embodiment of the present invention, specifically, the exhaled gas collection device includes an oral exhaled air collection module and a nasal exhaled air collection module, wherein: When sampling with the mouth-expiratory collection module, you need to pinch your nose and blow air into a disposable straw only with your mouth. When sampling with the nasal-expiratory collection module, you need to hold a fixing device in your mouth, place the head of the disposable straw on the outside of your nostrils, and exhale alternately through both nostrils.

[0020] In this preferred embodiment, the breath sample collection method is optimized based on two modes: oral exhalation mode and nasal exhalation mode, and two breath collection devices are built, namely oral exhalation and nasal exhalation. For oral exhalation sampling, the nose needs to be pinched, and air is blown into a disposable straw only with the mouth. For nasal exhalation sampling, a fixed device needs to be placed in the mouth, the disposable straw head is placed outside the nostril, and both nostrils are exhaled alternately. Compare the differences between oral exhalation and nasal exhalation, and adopt the breath collection setting with high prediction sensitivity, small external air interference, and good data repeatability under the current environment as needed.

[0021] As a preferred embodiment of the present invention, specifically, the flow-through medium passage discharge source is integrated with the GC-MS device, that is, the flow-through medium passage discharge source is connected in series with the GC-MS device and integrated in the same device housing.

[0022] In this preferred embodiment, the flow-through medium channel discharge source and the GC-MS device are "connected in series" in the above manner, and the components of the exhaled gas can be quickly predicted by simply collecting the exhaled gas from the human body, which is very convenient and efficient.

[0023] As a preferred embodiment of the present invention, the exhaled gas collection device further comprises: A disposable filter head is arranged at the front end of the disposable straw used for sampling by the oral exhalation collection module and the nasal exhalation collection module, and the disposable filter head is used to filter out suspended particles and water vapor in human exhaled air.

[0024] In this preferred embodiment, a disposable filter is installed at the front end of the straw to filter out suspended particles and most of the water vapor in the exhaled breath (it is known from previous experimental experience that the water vapor in the exhaled breath will condense and block the sample inlet) to avoid affecting the operating stability of the instrument and the test results.

[0025] As a preferred embodiment of the present invention, specifically, the ionized soft ionized gaseous molecules are analyzed by carbon dioxide isotope internal standard quantitative method to obtain prediction results, including: Determine the types of endogenous markers and the normal value range of each endogenous marker based on samples from a healthy population through preliminary experiments; Performing an endogenous marker quantitative analysis on the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a content value of a predetermined type of endogenous marker in the exhaled gas of a target human body; Determine whether the content value of any type of endogenous marker obtained by quantitative analysis is within its corresponding normal value range. If so, mark it as normal; if not and above the normal value range, mark it as abnormally high; if not and below the normal value range, mark it as abnormally low.

[0026] In this preferred embodiment, the 100 breath samples collected were first classified according to the research cohort, and the molecular ion peaks were identified and identified using the analysis software provided by the instrument, and quantitative analysis was performed using the standard gas external standard method. The content and relative proportion of common endogenous VOCs in the human body were studied, and a data array was established using control samples.

[0027] In order to organically combine breath analysis data with actual samples, multivariate statistical methods are an effective means of mathematical statistics, especially suitable for the analysis of complex high-throughput data, and are increasingly used in the study of breath characteristics. At present, the statistical methods involved include principal component analysis, cluster analysis, discriminant analysis, partial least squares analysis, etc., and the statistical analysis software involved includes SIMCA-P, SPSS, R, SAS, MATLAB, etc. This project intends to use SIMCA-P for principal component analysis and partial least squares analysis to find the corresponding characteristic substances of breath, and then use regression analysis and cluster analysis for data verification, and combine multiple statistical methods and statistical software for cross-validation to obtain more accurate and reliable analysis results.

[0028] Through data comparison and analysis, we finally screened out the characteristic endogenous VOCs in human exhaled breath and the relative content range of each compound, established an analysis model, and carried out experimental verification to determine the accuracy and sensitivity of the screening model. We established a dynamic, wide-range quantitative analysis method for exhaled VOCs based on "SOFT IONIZATION + DIRECT MASS".

[0029] As a preferred embodiment of the present invention, specifically, the ionized soft ionized gaseous molecules are subjected to endogenous marker quantitative analysis by carbon dioxide isotope internal standard quantitative method to obtain the content value of the predetermined endogenous marker type in the target human exhaled gas, including: The carbon dioxide isotope peak used for quantification of endogenous markers is determined by preliminary experiments; By comparing the input data after ionization with the abundance of each endogenous marker, the isotope peak with a peak intensity close to that of the endogenous marker in exhaled breath was selected as the reference standard, and the relative ratios of different markers to the carbon dioxide isotope peak were analyzed for quantification.

[0030] As a preferred embodiment of the present invention, specifically, the PC terminal visually displays the prediction results including: The name of each endogenous marker, the corresponding content value and the abnormal analysis situation are associated and displayed visually in the form of a chart.

[0031] As a preferred embodiment of the present invention, the GC-MS device, when debugging instrument parameters, includes: Through preliminary experiments, known endogenous volatile organic compounds commonly found in human exhaled breath are used as references for testing and analysis, and instrument parameters are debugged, including sensitivity.

[0032] Embodiment 2, the present invention further proposes a quantitative prediction method for endogenous volatile organic compounds in human exhaled breath, which is applied to the quantitative prediction system for endogenous volatile organic compounds in human exhaled breath, comprising: Acquire the exhaled gas of the target human body through an exhaled gas collection device; The discharge source receives the exhaled gas of the target human body through the flow-through medium channel, and acts on the exhaled gas of the target human body to output soft ionized gaseous molecules; Ionizing the soft ionized gaseous molecules by a GC-MS device, and analyzing the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a prediction result; The prediction results are obtained through a PC device and visualized.

[0033] Although the description of the present invention has been quite detailed and specifically describes several described embodiments, it is not intended to be limited to any of these details or embodiments or any particular embodiment, but should be regarded as providing a broad possible interpretation of these claims in view of the prior art by reference to the appended claims, thereby effectively covering the intended scope of the present invention. In addition, the above description of the present invention is based on the embodiments foreseeable by the inventor, and its purpose is to provide a useful description, and those non-substantial changes to the present invention that have not yet been foreseen may still represent equivalent changes to the present invention.

[0034] The above is only a preferred embodiment of the present invention. The present invention is not limited to the above implementation. As long as the technical effect of the present invention is achieved by the same means, it should belong to the protection scope of the present invention. Within the protection scope of the present invention, its technical scheme and / or implementation method can have various modifications and changes.

Claims

1. A quantitative prediction system for endogenous volatile organic compounds in human exhaled breath, characterized in that: Includes the following: An exhaled gas collection device, used to obtain exhaled gas from a target human body; A flow-through medium channel discharge source, the input end of which is directly connected to the output end of the exhaled gas collection device, for receiving the exhaled gas of the target human body and acting on the exhaled gas of the target human body to output soft ionized gaseous molecules; A GC-MS device, the input end of which is directly connected to the output end of the flow-through medium passage discharge source, is used to ionize the soft ionized gaseous molecules, and analyze the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a prediction result; The PC device is connected to the GC-MS device for obtaining the prediction results and visually displaying the prediction results.

2. The quantitative prediction system for endogenous volatile organic compounds in human exhaled breath according to claim 1, characterized in that: Specifically, the exhaled gas collection device includes an oral exhaled gas collection module and a nasal exhaled gas collection module, wherein: When sampling with the mouth-expiratory collection module, you need to pinch your nose and blow air into a disposable straw only with your mouth. When sampling with the nasal-expiratory collection module, you need to hold a fixing device in your mouth, place the head of the disposable straw on the outside of your nostrils, and exhale alternately through both nostrils.

3. The quantitative prediction system for endogenous volatile organic compounds in human exhaled breath according to claim 1, characterized in that: Specifically, the flow-through medium passage discharge source and the GC-MS device are integrated, that is, the flow-through medium passage discharge source and the GC-MS device are connected in series and integrated in the same device housing.

4. The quantitative prediction system for endogenous volatile organic compounds in human exhaled breath according to claim 2, characterized in that: The exhaled gas collection device further comprises: A disposable filter head is arranged at the front end of the disposable straw used for sampling by the oral exhalation collection module and the nasal exhalation collection module, and the disposable filter head is used to filter out suspended particles and water vapor in human exhaled air.

5. The quantitative prediction system for endogenous volatile organic compounds in human exhaled breath according to claim 1, characterized in that: Specifically, the ionized soft ionized gaseous molecules are analyzed by carbon dioxide isotope internal standard quantitative method to obtain prediction results, including: Determine the types of endogenous markers and the normal value range of each endogenous marker based on samples from a healthy population through preliminary experiments; Performing an endogenous marker quantitative analysis on the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a content value of a predetermined type of endogenous marker in the exhaled gas of a target human body; Determine whether the content value of any type of endogenous marker obtained by quantitative analysis is within its corresponding normal value range. If so, mark it as normal; if not and above the normal value range, mark it as abnormally high; if not and below the normal value range, mark it as abnormally low.

6. The quantitative prediction system for endogenous volatile organic compounds in human exhaled breath according to claim 5, characterized in that: Specifically, the ionized soft ionized gaseous molecules are subjected to endogenous marker quantitative analysis by carbon dioxide isotope internal standard quantitative method to obtain the content value of the predetermined endogenous marker type in the target human exhaled gas, including: The carbon dioxide isotope peak used for quantification of endogenous markers is determined by preliminary experiments; By comparing the input data after ionization with the abundance of each endogenous marker, the isotope peak with a peak intensity close to that of the endogenous marker in exhaled breath was selected as the reference standard, and the relative ratios of different markers to the carbon dioxide isotope peak were analyzed for quantification.

7. The quantitative prediction system for endogenous volatile organic compounds in human exhaled breath according to claim 5, characterized in that: Specifically, the PC terminal performs a visual display of the prediction result including: The name of each endogenous marker, the corresponding content value and the abnormal analysis situation are associated and displayed visually in the form of a chart.

8. The quantitative prediction system for endogenous volatile organic compounds in human exhaled breath according to claim 1, characterized in that: When debugging the instrument parameters of the GC-MS device, the following steps are included: Through preliminary experiments, known endogenous volatile organic compounds commonly found in human exhaled breath are used as references for testing and analysis, and instrument parameters are debugged, including sensitivity.

9. A quantitative prediction method for endogenous volatile organic compounds in human exhaled breath, characterized in that: A quantitative prediction system for endogenous volatile organic compounds in human exhaled breath as described in any one of claims 1 to 8, comprising: Acquire the exhaled gas of the target human body through an exhaled gas collection device; The discharge source receives the exhaled gas of the target human body through the flow-through medium channel, and acts on the exhaled gas of the target human body to output soft ionized gaseous molecules; Ionizing the soft ionized gaseous molecules by a GC-MS device, and analyzing the ionized soft ionized gaseous molecules by a carbon dioxide isotope internal standard quantitative method to obtain a prediction result; The prediction results are obtained through a PC device and visualized.

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