Method for evaluating fertilizer efficiency based on model plant arabidopsis thaliana

Abstract

The invention relates to a method for evaluating fertilizer efficiency based on a model plant arabidopsis thaliana. The method comprises the following steps: acquiring culture condition information of the model plant arabidopsis thaliana; setting parameter information of an artificial climate box based on the culture conditions, and culturing the model plant arabidopsis thaliana; determining evaluation indexes of growth of model plant arabidopsis thaliana, wherein the evaluation indexes comprise one or more of dimension indexes of roots, stems, flowers, leaves and fruits; and collecting and counting the growth data of each dimension, evaluating the effect of the fertilizer, and determining the dominant action part of the fertilizer. Compared with the prior art, the fertilizer efficiency evaluation method provided by the invention has the advantages that a precise and efficient fertilizer effect test means is provided by performing multi-dimensional systematic evaluation on the model plant arabidopsis thaliana under the control condition; the growth data of roots, stems, flowers, leaves and fruits of plants can be comprehensively obtained in a single test period, and the comprehensiveness and accuracy of evaluation are ensured.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizer effect evaluation, and in particular to a method for evaluating fertilizer effect based on a model plant Arabidopsis thaliana. Background Art

[0002] Fertilizer is an important agricultural production material. Scientific evaluation of fertilizer application effect is of great significance for improving fertilization technology, improving fertilizer resource utilization efficiency, achieving agricultural production and efficiency increase, and ensuring sustainable agricultural development. The main methods and indicators for evaluating fertilizer application effect include fertilizer utilization rate, fertilizer agronomic efficiency, fertilizer partial productivity, etc.

[0003] In addition to verifying the effect of fertilizers by measuring fertilizer utilization rate and fertilizer partial productivity, plant growth tests are also often used to verify the effect of fertilizers after application. The potted crops that are more involved in the literature include leafy crops such as lettuce and pakchoi; field crops such as rice and corn; and crops used for special fertilizers such as flowers, specific vegetables and fruits.

[0004] There are few patents related to fertilizer efficiency evaluation. Among the patents found, patent CN201610044676.X discloses a method of evaluating zinc fertilizer by quantitatively analyzing the zinc in the tested part of the marked leaves by marking leaves with zinc isotope solution; patent CN201410700860.6 discloses a method of evaluating fertilizer efficiency by comparing soil nutrients before and after crop harvest and calculating fertilizer efficiency; patent CN202010541838.7 screens whether wheat is a nitrogen efficient utilization variety by calculating nitrogen fertilizer utilization rate, nitrogen fertilizer agronomic efficiency, nitrogen fertilizer partial productivity and other data. However, the evaluation methods used in the above patents involving fertilizer efficiency evaluation are completely different from the method described in this patent in terms of crops used and evaluation indicators.

[0005] The commonly used organs for evaluating the fertilizer effect of crops themselves can be divided into five dimensions: roots, stems, flowers, leaves, and fruits. According to the existing relevant literature and patents in my country, in addition to the development of special crop fertilizers, which requires the use of designated crops for fertilizer effect tests, leafy vegetables are most commonly used as representatives to verify the effect of fertilizers, because leafy vegetable crops are easy to obtain, have a short planting cycle, require a relatively small area for planting, and have a relatively low overall planting cost. Commonly used measurement indicators include fresh weight of leafy vegetables, root length, root weight, and chlorophyll content, with a focus on indicators in the two dimensions of roots and leaves. In addition, crops such as corn and rice are used to evaluate the fruit dimension, and flowers are used to evaluate the flower dimension.

[0006] Different crops have different functional requirements for fertilizers. For example, potato crops need good root expansion; sugarcane crops need to control the growth of stems to be moderate; flower crops need to control the quality of flowers; tea and other crops need better leaf taste; apples, pears and other fruit crops need to expand fruits, etc. According to the actual application of fertilizers, there are some problems with the current evaluation methods. For example, when evaluating the effect of fertilizers with leafy crops, only the roots and leaves can be evaluated in one test, and the evaluation dimensions are not comprehensive enough; for example, when using crops such as corn, rice, flowers, fruits and vegetables, the test cycle of one test is long, the planting area required is large, and the labor cost and management cost are high.

[0007] With the acceleration of agricultural modernization and the increasing requirements for precision fertilization, there is an urgent need for a fertilizer efficiency evaluation method that can take into account comprehensiveness, high efficiency and low cost while ensuring the accuracy of the evaluation. Existing evaluation methods cannot meet the needs of quickly screening a large number of fertilizer formulas and accurately determining the effects of fertilizers on different plant parts. This greatly limits the development speed and promotion of new fertilizers, and it is difficult to adapt to the long-term needs of sustainable agricultural development. Therefore, how to find a suitable model plant and a supporting evaluation system to solve these problems in the current fertilizer efficiency evaluation has become a key technical problem that needs to be overcome in this field. Summary of the invention

[0008] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and to provide a method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana. By conducting a multi-dimensional systematic evaluation of the model plant Arabidopsis thaliana under controlled conditions, an accurate and efficient means of testing the fertilizer effect is provided.

[0009] The present invention is considered in the process of conception:

[0010] The present invention uses the "fruit fly" in plants-Arabidopsis to evaluate the effect of fertilizers. Arabidopsis is a common crop in plant genetic research and is also a model plant that is currently studied more thoroughly. Arabidopsis plants are small, close to the same size as leafy vegetables, saving planting space; one Arabidopsis plant contains five dimensions of roots, stems, leaves, flowers, and fruits at the same time, and one test can obtain the fertilizer effect of five dimensions at the same time. In the early stage of fertilizer development and application, fertilizer effect evaluation tests are mostly carried out in laboratories or in small-area planting demonstration fields, and the cost of the test site needs to be controlled. Compared with crops such as corn and flowers, Arabidopsis has a shorter planting cycle and can bloom and bear fruit in 7-8 weeks; Arabidopsis survives better and can be planted in an artificial climate box, the planting conditions are controllable and the required planting site is small. In summary, using Arabidopsis for fertilizer effect evaluation tests can save time costs and management costs such as site labor while obtaining multi-dimensional evaluation indicators. Fertilizer effect evaluation using Arabidopsis has the significant advantages of high efficiency and low cost. Compared with leafy crops or other commonly used field crops, the evaluation data obtained by evaluating fertilizer efficiency using Arabidopsis thaliana is highly comparable and repeatable, providing a large amount of data for subsequent fertilizer applications in a short period of time, which can be used to establish a standardized database and improve the efficiency of fertilizer product development. Through multiple tests, the advantageous dimensions of the developed fertilizer can be obtained to guide the research and development and application of fertilizers.

[0011] The purpose of the present invention can be achieved by the following technical solutions:

[0012] The present invention provides a method for evaluating fertilizer efficiency based on a model plant Arabidopsis thaliana, comprising the following steps:

[0013] S1: Obtain information on the culture conditions of Arabidopsis thaliana;

[0014] S2: Based on the culture condition information, setting parameter information of the artificial climate box to culture Arabidopsis thaliana;

[0015] S3: Setting an evaluation index for Arabidopsis growth, wherein the evaluation index includes one or more dimensional indexes of roots, stems, flowers, leaves, and fruits;

[0016] S4: Collect and compile statistics on the growth data of Arabidopsis thaliana in various dimensions, evaluate the effect of fertilizers, and determine the dominant sites of action of fertilizers on Arabidopsis thaliana.

[0017] Furthermore, in S1, the culture condition information includes the vernalization conditions of Arabidopsis seeds, the specific substrate type required for planting, the planting density, the culture solution product necessary for planting, the amount of culture solution, the time of adding the culture solution, and the time of adding the fertilizer preparation required to be evaluated.

[0018] Furthermore, in the culture condition information, the vernalization treatment condition of the seeds is 0-5°C, and the vernalization time is 48h-96h;

[0019] The soilless cultivation substrate used is 1-3 mm vermiculite and 2-5 mm quartz sand, and the vermiculite and quartz sand are evenly mixed in a ratio of 2:1.

[0020] Furthermore, the planting density should provide a growth space of no less than 80 mm*80 mm*100 mm for each Arabidopsis plant;

[0021] The culture liquid product necessary for planting is 1 / 2MS culture medium, and the 1 / 2MS culture medium does not contain sucrose and agar;

[0022] The amount of culture solution used for each Arabidopsis plant is 20ml-30ml;

[0023] The culture solution was added at the following time: the culture solution was poured when the Arabidopsis thaliana was 2 weeks old, and then once a week at a fixed time, for a total of 4 times.

[0024] Furthermore, the required fertilizer preparation for evaluation is watered when the Arabidopsis thaliana grows to 3 weeks old, and then watered once a week at a fixed time, for a total of 5 times, and the time interval between adding the culture solution and the required fertilizer preparation for evaluation is not less than 2-3 days.

[0025] Further, in S2, the parameter information of the artificial climate box includes temperature, humidity, and light conditions;

[0026] The temperature of the artificial climate chamber was set at 25°C under light conditions and 21°C under dark conditions;

[0027] Humidity is 70%-75%; light intensity is 5000-6000lux.

[0028] Furthermore, in S3, the process of determining the evaluation index of Arabidopsis thaliana growth includes:

[0029] determining root growth indicators, including one or more of root length, root fresh weight, root dry weight, and root quantity;

[0030] determining stem growth indicators, including one or more of stem length, stem fresh weight, and stem dry weight;

[0031] determining leaf growth indicators, including one or more of leaf number, rosette diameter, leaf fresh weight, leaf dry weight, leaf area, chlorophyll content, and photosynthesis efficiency;

[0032] Determine the growth index of flowers, including one or more of the number of flowers and the diameter of flowers;

[0033] Determine the growth index of the fruit, including one or more of the number of fruits, fruit size, fruit fresh weight, fruit dry weight, and fruit nutritional components.

[0034] Furthermore, in S3, the process of determining the evaluation index of the model plant growth also includes the data collection time, and the five-dimensional index data are collected when the Arabidopsis grows to 55-60 days.

[0035] Furthermore, in S4, the growth data of Arabidopsis thaliana in five dimensions, namely, root, stem, flower, leaf, and fruit, were comprehensively obtained within a single experimental cycle.

[0036] Furthermore, in S4, the type of fertilizer includes one of inorganic fertilizer, organic fertilizer, microbial fertilizer, and biostimulant.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] 1) The method of the present invention is obviously advanced. The fertilizer effect evaluation method of the present invention provides an accurate and efficient means of testing the effect of fertilizers by conducting a multi-dimensional systematic evaluation of the model plant Arabidopsis thaliana under controlled conditions. This method can comprehensively obtain the growth data of the roots, stems, flowers, leaves, and fruits of the plant within a single test cycle, ensuring the comprehensiveness and accuracy of the evaluation. Since the test is carried out in an artificial climate chamber, planting conditions such as temperature, humidity, light, and culture medium can be precisely controlled, thereby reducing the interference of environmental variables, improving the reliability of the test, and making the evaluation results more stable and repeatable.

[0039] 2) The method of the present invention has significant economic advantages. Since the model plant Arabidopsis has a short growth cycle and requires less space and conditions for planting, the time and site costs required are greatly reduced. This not only improves the efficiency of fertilizer evaluation, but also makes frequent and large-scale fertilizer testing possible, which helps to quickly screen and optimize fertilizer formulas, thereby promoting the research and development and improvement of fertilizer products.

[0040] 3) The method of the present invention has certain reliability. Among the seed vernalization treatment conditions, the specific substrate types required for planting, the planting density, the culture solution products required for planting, the amount of culture solution, the time of adding the culture solution, the time of adding the fertilizer preparation required for evaluation, the determination of the growth evaluation index and the data acquisition method used in the invention, the conditions proposed by the present invention are the optimal conditions determined on the basis of repeated experiments, and these conditions are more conducive to the growth test of Arabidopsis and the collection of data. DETAILED DESCRIPTION

[0041] The present invention is described in detail below in conjunction with specific embodiments. Component models, material names, connection structures, control methods, algorithms and other features not clearly described in this technical solution are all considered to be common technical features disclosed in the prior art.

[0042] Example 1

[0043] This embodiment provides a method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana, comprising the following steps:

[0044] S1: Obtain the culture conditions of Arabidopsis thaliana;

[0045] In S1, the culture conditions include the vernalization conditions of Arabidopsis seeds, the specific substrate type required for planting, the planting density, the culture solution product necessary for planting, the amount of culture solution, the time of adding the culture solution, and the time of adding the fertilizer preparation required to be evaluated.

[0046] S1 of this embodiment involves collecting the culture conditions of Arabidopsis thaliana, ensuring the precise control of the planting process and the scientific management of plant growth conditions. The principle of this step is based on a deep understanding of the basic conditions for plant growth, including seed pretreatment, material specifications and nutrient supply required for planting Arabidopsis thaliana.

[0047] Vernalization of seeds is a biological process that simulates winter conditions in the natural environment by exposing them to a specific temperature for a period of time to promote seed germination and growth. In the present invention, the vernalization temperature of Arabidopsis seeds is 0-5°C, and the temperature range is acceptable. The vernalization time is 48h-96h, and the longest time is no more than 96h.

[0048] Soilless culture is a cultivation method that uses culture solution containing essential elements for plant growth and development to provide nutrition without using natural soil. The present invention relates to fertilizer efficiency evaluation. Compared with soil, soilless culture can avoid the influence of complex nutrients and microorganisms in the soil on plant growth, making the evaluation result more controllable. Soilless culture is divided into hydroponics and matrix culture. The present invention adopts matrix culture, and the commonly used materials for matrix culture are rock wool, perlite, vermiculite, coconut bran, etc. Compared with hydroponics, matrix culture has low equipment requirements and is easier for beginners to master. The soilless culture matrix used in the present invention is vermiculite (1-3 mm) and quartz sand (2-5 mm). Vermiculite and quartz sand are evenly mixed in a ratio of 2:1 as the growth matrix of Arabidopsis thaliana. Both materials have good moisturizing effects. The addition of quartz sand will make the entire matrix more breathable and loose, which is more conducive to the growth of Arabidopsis thaliana roots. The seeds of Arabidopsis thaliana are relatively small (1-3 mm) and can be selected within the material specifications provided by the present invention. The material specifications should not be too large, which is not conducive to the germination of Arabidopsis thaliana. In actual operation, the mixed matrix is ​​fully moistened with sterile water or deionized water, and the matrix does not drip and the surface is moist. Then, the Arabidopsis thaliana seeds that have been vernalized are dotted on the surface of the matrix with a dropper, a pipette gun or a toothpick. During germination, water should be replenished in time to keep the surface of the matrix moist.

[0049] The leaf spread (rosette diameter) of Arabidopsis thaliana was 7-15 cm on average during the test period, so the growth space required for each Arabidopsis thaliana plant should be no less than 80 mm*80 mm*100 mm.

[0050] The culture medium preparation necessary for the growth of Arabidopsis thaliana is 1 / 2 MS medium (without sucrose and agar).

[0051] The amount of culture solution used for each Arabidopsis plant is 20ml-30ml, which can be adjusted appropriately according to the size of the culture container. When the Arabidopsis grows to 2 weeks old, water the culture solution, and then water it once a week at a fixed time, for a total of 4 times; when the Arabidopsis grows to 3 weeks old, water it with the required fertilizer preparation, and then water it once a week at a fixed time, for a total of 5 times. It should be noted that the time interval between the addition of the culture solution and the required fertilizer preparation should not be less than 2-3 days.

[0052] S2: Based on the culture conditions, setting control information of the artificial climate box to culture Arabidopsis thaliana;

[0053] In S2, the parameter information of the artificial climate box includes temperature, humidity, and light conditions. The artificial climate box is set to a temperature of 25°C under light conditions and 21°C under dark conditions; humidity is 70%-75%; and light intensity is 5000-6000 lux. In actual operation, the light condition time is 16 hours, and the dark condition time is 8 hours.

[0054] The principle of S2 is to use an artificial climate chamber to simulate and control the optimal environmental conditions for plant growth. By setting precise temperature, humidity and light conditions, a stable and optimal growth environment is provided for Arabidopsis. The dynamic adjustment of these conditions is based on the plant's response to changes in environmental factors at different growth stages, ensuring maximum plant growth and accurate evaluation of fertilizer effects. This environmental control method eliminates the interference of unstable external climate, making growth data more accurate and fertilizer effect evaluation more reliable.

[0055] S3: Determine an evaluation index for Arabidopsis growth, wherein the evaluation index includes one or more dimensional indexes of roots, stems, flowers, leaves, and fruits;

[0056] In S3, the process of determining the evaluation index of Arabidopsis growth includes:

[0057] determining root growth indicators, including one or more of root length, root fresh weight, root dry weight, and root quantity;

[0058] determining stem growth indicators, including one or more of stem length, stem fresh weight, and stem dry weight;

[0059] determining leaf growth indicators, including one or more of leaf number, rosette diameter, leaf fresh weight, leaf dry weight, leaf area, chlorophyll content, and photosynthesis efficiency;

[0060] Determine the growth index of flowers, including one or more of the number of flowers and the diameter of flowers;

[0061] Determine the growth index of the fruit, including one or more of the number of fruits, fruit size, fruit fresh weight, fruit dry weight, and fruit nutritional components.

[0062] In S3, the process of determining the evaluation index of Arabidopsis growth also includes designing data collection methods, including:

[0063] Select data collection tools suitable for various indicators. In the present invention, the length, height, and diameter indicators are measured with a ruler; the fresh weight and dry weight indicators are weighed with an analytical balance; the quantity indicators are counted by counting method; the area, material content, light and efficiency, and nutrient component indicators are detected with professional instruments. Users can select indicators for detection according to the functional requirements of the fertilizer to be evaluated.

[0064] The frequency and timing of data collection were determined to ensure that measurements were taken at key stages of plant growth. Data on the five dimensions were collected when Arabidopsis thaliana grew to 55-60 days.

[0065] In S3, the process of determining the evaluation index of Arabidopsis growth also includes establishing the quantitative standard of the evaluation index, which specifically includes:

[0066] Determine the weight of the indicators. If some indicators are more critical to the evaluation of fertilizer efficiency, they will be given higher weights. For example, in the root dimension, three indicators, root length, root weight, and root number, are selected for root dimension evaluation. The user can set the weight ratio of root length to 30%, root weight to 50%, and root number to 20%. According to the weight ratio, a comprehensive value can be obtained, which can represent the comprehensive effect of a certain fertilizer on the root. The user can set different weight ratios for calculation according to the function of the fertilizer to be evaluated, which is used for the comprehensive evaluation of a certain dimension.

[0067] In S3, the process of determining the evaluation index of Arabidopsis growth also includes the formulation of data recording and analysis plan, including:

[0068] Design data logging forms or databases to record all collected data.

[0069] Select data analysis tools to process and analyze the collected data. Usually, Excel is used for statistics and basic analysis of the data, and SPSS is used for differential analysis of the data.

[0070] The key of S3 of this embodiment is to establish a comprehensive plant growth evaluation system, and ensure a comprehensive evaluation of the plant growth status by selecting key dimensional indicators of roots, stems, flowers, leaves and fruits. The principle of this step is based on the basic knowledge of botany, that is, the growth status of plants can be comprehensively reflected by the development of their different organs. Indicators such as the length and weight of the root are selected because the root system is the main organ for plants to absorb water and nutrients, and its development status is directly related to the growth and health of the plant. The weight and length of the stem reflect the structural support and growth rate of the plant. Indicators such as the number and weight of leaves, chlorophyll content, and photosynthesis efficiency are important indicators for evaluating the energy conversion capacity of plants. The number of flowers is an important indicator of plant reproductive capacity, and the diameter of the flower is an important indicator for measuring the growth rate of plant reproductive organs and the economic value of flowers. Indicators such as the number and weight of fruits are directly related to the reproductive success rate, nutritional value and economic value of plants. Through the comprehensive analysis of these indicators, the key effects of fertilizers on plant growth can be accurately evaluated, and the dominant action sites of fertilizers at different growth stages can be determined. In addition, this step also includes designing data collection methods to ensure the accuracy and comparability of data, as well as establishing data recording and analysis programs to achieve accurate monitoring and evaluation of plant growth. These methods provide a scientific basis for the evaluation of fertilizer effects and help optimize fertilizer formulations and application strategies.

[0071] S4: Collect and compile growth data in various dimensions, evaluate the effect of fertilizers, and determine the dominant sites of action of fertilizers.

[0072] S4 specifically includes the following steps:

[0073] Collect growth data of roots, stems, flowers, leaves, and fruits of Arabidopsis;

[0074] Perform data cleaning and preprocessing to ensure data quality. When processing data, you can remove the maximum and minimum values ​​and values ​​with large differences and then take the average value.

[0075] Use statistical methods to analyze data and find out the impact of fertilizers on various growth dimensions;

[0076] Evaluate fertilizer effectiveness and determine the role of fertilizers in different growth dimensions.

[0077] In S4, the specific process of collecting the growth data of roots, stems, flowers, leaves, and fruits of Arabidopsis thaliana includes:

[0078] Collect physical and physiological data of roots, stems, flowers, leaves, and fruits of Arabidopsis at different growth stages.

[0079] Use standardized data collection tools and methods to ensure data consistency and comparability,

[0080] Record the environmental and culture conditions, including temperature, humidity, light, vernalization temperature and time, amount of culture solution added, composition and amount of fertilizer preparations, and time of adding culture solution and fertilizer preparations.

[0081] In S4, the specific process of using statistical methods to analyze data and find out the effect of fertilizer on each growth dimension includes at least one of the following processes:

[0082] Use charts and graphs to visually display the distribution and trends of data, which can be represented using bar charts or radar charts.

[0083] Generate growth curves to compare the growth of Arabidopsis thaliana under different fertilizer treatments.

[0084] In S4, the types of fertilizers that can be evaluated by this patent include inorganic fertilizers, organic fertilizers, microbial fertilizers, and biostimulants. Fertilizer products are dissolved in sterile water or deionized water and then added.

[0085] The principle of S4 is to use precise data collection and statistical analysis to evaluate the effect of fertilizers. First, physical and physiological data of Arabidopsis roots, stems, flowers, leaves, and fruits are collected through standardized tools, which reflect the plant's response to fertilizer nutrients. Data collection not only includes direct measurement of plant growth, but also involves recording environmental and culture conditions, ensuring data consistency and comparability. Next, data cleaning and preprocessing are used to ensure data quality and lay the foundation for accurate analysis. Then, statistical methods are used to analyze the data to identify the specific effects of fertilizers on various growth dimensions of plants, which involves key physiological processes such as plant growth rate and biomass allocation. Finally, through comprehensive analysis, the effect of fertilizers is evaluated and its role in different growth dimensions of plants is determined, providing a scientific basis for the optimization of fertilizer formulations. In addition, S4 can also include the use of charts and graphs to intuitively display the distribution and trend of data, generate growth curves, compare the growth of Arabidopsis under different fertilizer treatments, and make bar charts or radar charts to display growth data and fertilizer effects in different dimensions. These visualization tools not only help researchers understand the data more intuitively, but also reveal patterns and trends in the data, thereby more accurately evaluating the dominant sites of action of fertilizers. In addition, S4 also includes fertilizer categories that can be evaluated to better serve the evaluation and development of fertilizer products.

[0086] Application Example 1

[0087] S1. Arabidopsis seedling cultivation: The Arabidopsis seeds used were Columbia wild type. The Arabidopsis seeds required for the experiment were rinsed with sterile water three times, protected from light, and placed in a 4°C refrigerator for vernalization for 48 hours. Vermiculite (1-3 mm) and quartz sand (2-5 mm) were mixed in a ratio of 2:1 and evenly placed in a flowerpot (8cm*8cm*10cm). The surface was flattened and placed in a tray (35.6cm*26cm). Sterile water was added to the bottom of the tray to make the vermiculite surface absorb water and moisten it. Use a toothpick to place the vernalized Arabidopsis seeds on the moist substrate surface. During the germination process, water was added in time to keep the substrate surface moist.

[0088] S2. Arabidopsis cultivation: The cultivation process is carried out in an artificial climate chamber, and the parameters are set as follows: temperature and time are 25°C / 16h during the day, 21°C / 8h at night, humidity is 70%, and light intensity is 5000lx. The above tray is placed in the incubator, and when the Arabidopsis grows to 2 weeks, the culture solution is irrigated with 20ml / flower pot, and then irrigated once a week at a fixed time, and irrigated 4 times throughout the process. When the Arabidopsis grows to 3 weeks, the required biostimulant preparation for evaluation is irrigated, and then irrigated once a week at a fixed time, and irrigated 5 times throughout the process.

[0089] S3. Effect evaluation index monitoring: The root dimension is based on the root fresh weight index; the stem dimension is based on the stem length index; the flower dimension is based on the number of flowers; the leaf dimension is based on the number of leaves; the fruit dimension is based on the fruit fresh weight index. When Arabidopsis grows to 55 days, the results of the five dimensional indicators are statistically analyzed. When statistics are performed, 10 plants with uniform growth are selected for each sample treatment; when analyzing each dimension, the maximum, minimum and difference values ​​are removed, and the average value of each dimension is used as the judgment value. According to the statistical results, the dominant action site of the evaluated fertilizer is analyzed.

[0090] As shown in the table below, among the three samples, sample 3 has better growth-promoting effects on five dimensions; sample 2 has better growth-promoting effects on roots, stems and flowers; and sample 1 only has a growth-promoting effect on stems and flowers.

[0091] Table 1 Effects of different biostimulants

[0092]

[0093]

[0094] Application Example 2

[0095] S1. Arabidopsis seedling cultivation: The Arabidopsis seeds used were Columbia wild type. The Arabidopsis seeds required for the experiment were rinsed with sterile water for 3 times, protected from light, and placed in a 4°C refrigerator for vernalization for 72 hours. Vermiculite (1-3 mm) and quartz sand (2-5 mm) were mixed in a ratio of 2:1 and evenly placed in a flowerpot (8cm*8cm*10cm). The surface was flattened and placed in a tray (35.6cm*26cm). Sterile water was added to the bottom of the tray to make the vermiculite surface absorb water and moisten it. Use a toothpick to place the vernalized Arabidopsis seeds on the moist substrate surface. During the germination process, water was added in time to keep the substrate surface moist.

[0096] S2. Arabidopsis cultivation: The cultivation process is carried out in an artificial climate box, and the parameters are set as follows: temperature time is 25°C / 16h during the day, 21°C / 8h at night, humidity is 75%, and light intensity is 6000lx. The above tray is placed in the incubator, and when the Arabidopsis grows to 2 weeks, the culture solution is irrigated with 20ml / flower pot, and then irrigated once a week at a fixed time, and irrigated 4 times throughout the process. When the Arabidopsis grows to 3 weeks, the required potassium fertilizer preparation is irrigated, and then irrigated once a week at a fixed time, and irrigated 5 times throughout the process.

[0097] S3. Effect evaluation index monitoring: The root dimension is based on the root fresh weight index; the stem dimension is based on the stem length index; the flower dimension is based on the number of flowers; the leaf dimension is based on the number of leaves; the fruit dimension is based on the fruit fresh weight index. When Arabidopsis grows to 55 days, the results of the five dimensional indicators are statistically analyzed. When statistics are performed, 7 plants with uniform growth are selected for each sample treatment; when analyzing each dimension, the maximum, minimum and difference values ​​are removed, and the average value of each dimension is used as the judgment value. According to the statistical results, the dominant action parts of different potassium fertilizers are analyzed.

[0098] As shown in the table below, sample 1 has the best effect in promoting growth in both stems and leaves.

[0099] Table 2 Effects of different potassium fertilizers

[0100]

[0101]

[0102] Application Example 3

[0103] S1. Arabidopsis seedling cultivation: The Arabidopsis seeds used were Columbia wild type. The Arabidopsis seeds required for the experiment were rinsed with sterile water three times, protected from light, and placed in a 5°C refrigerator for vernalization for 96 hours. Vermiculite (1-3 mm) and quartz sand (2-5 mm) were mixed in a ratio of 2:1 and evenly placed in a flowerpot (8cm*8cm*10cm). The surface was flattened and placed in a tray (35.6cm*26cm). Sterile water was added to the bottom of the tray to make the vermiculite surface absorb water and moisten it. Use a pipette to place the vernalized Arabidopsis seeds on the moist substrate surface. During the germination process, water was added in time to keep the substrate surface moist.

[0104] S2. Arabidopsis cultivation: The cultivation process is carried out in an artificial climate box, and the parameters are set as follows: temperature time is 25℃ / 16h during the day, 21℃ / 8h at night, humidity is 75%, and light intensity is 6000lx. The above tray is placed in the incubator, and when the Arabidopsis grows to 2 weeks, the culture solution is irrigated with 20ml / flower pot, and then irrigated once a week at a fixed time, and irrigated 4 times throughout the process. When the Arabidopsis grows to 3 weeks, the required potassium fertilizer preparation is irrigated, and then irrigated once a week at a fixed time, and irrigated 5 times throughout the process.

[0105] S3. Effect evaluation index monitoring: The root dimension is based on the root length index; the stem dimension is based on the stem fresh weight index; the flower dimension is based on the number of flowers; the leaf dimension is based on the rosette diameter; the fruit dimension is based on the fruit weight index. When Arabidopsis grows to 55 days, the results of the five dimensional indicators are statistically analyzed. When statistics are performed, 7 plants with uniform growth are selected for each sample treatment; when analyzing each dimension, the maximum, minimum and difference values ​​are removed, and the average value of each dimension is used as the judgment value. According to the statistical results, the dominant action site of the evaluated fertilizer is analyzed.

[0106] As shown in the table below, among the five samples, sample 2 has the best growth-promoting effect on root length, there is no obvious difference in the growth-promoting effect of the five samples on leaves, and sample 3 has the best growth-promoting effect on stems, and also has a growth-promoting effect on fruits.

[0107] Table 3 Effects of different potassium fertilizers

[0108]

[0109]

[0110] Application Example 4

[0111] S1. Arabidopsis seedling cultivation: The Arabidopsis seeds used were Columbia wild type. The Arabidopsis seeds required for the experiment were rinsed with sterile water three times, protected from light, and placed in a 5°C refrigerator for vernalization for 48 hours. Vermiculite (1-3 mm) and quartz sand (2-5 mm) were mixed in a ratio of 2:1 and evenly placed in a flowerpot (10 cm*10 cm*10 cm). The surface was flattened and placed in a tray (40 cm*30 cm). Sterile water was added to the bottom of the tray to make the vermiculite surface absorb water and moisten it. Use a toothpick to place the vernalized Arabidopsis seeds on the moist substrate surface. During the germination process, water was added in time to keep the substrate surface moist.

[0112] S2. Arabidopsis cultivation: The cultivation process is carried out in an artificial climate chamber, and the parameters are set as follows: temperature and time are 25°C / 16h during the day, 21°C / 8h at night, humidity is 72%, and light intensity is 6000lx. The above tray is placed in the incubator, and when the Arabidopsis grows to 2 weeks, 30ml / flower pot of culture solution is watered, and then irrigated once a week at a fixed time, and irrigated 4 times throughout the process. When the Arabidopsis grows to 3 weeks, the required biostimulant preparation for evaluation is watered, and then irrigated once a week at a fixed time, and irrigated 5 times throughout the process.

[0113] S3. Monitoring of effect evaluation indicators: The root dimension is based on the root length indicator; the stem dimension is based on the stem length indicator; the flower dimension is based on the flower diameter; the leaf dimension is based on the number of leaves; the fruit dimension is based on the fruit fresh weight indicator. When Arabidopsis grows to 55 days, the results of the five dimensional indicators are statistically analyzed. When statistics are performed, 8 plants with uniform growth are selected for each sample treatment; when analyzing each dimension, the maximum, minimum and difference values ​​are removed, and the average value of each dimension is used as the judgment value. According to the statistical results, the dominant action site of the evaluated fertilizer is analyzed.

[0114] As shown in the following table, among the three samples, sample 1 has a poorer overall plant growth-promoting effect compared with the other two samples, sample 2 has a better overall plant growth-promoting effect compared with sample 1, and sample 3 has a better growth-promoting effect on stems and leaves.

[0115] Table 4 Effects of different biostimulants

[0116]

[0117]

[0118] Application Example 5

[0119] S1. Arabidopsis seedling cultivation: The Arabidopsis seeds used were Columbia wild type. The Arabidopsis seeds required for the experiment were rinsed with sterile water for 3 times, protected from light, and placed in a 3°C refrigerator for vernalization for 48 hours. Vermiculite (1-3 mm) and quartz sand (2-5 mm) were mixed in a ratio of 2:1 and evenly placed in a flowerpot (10 cm*10 cm*10 cm). The surface was flattened and placed in a tray (40 cm*30 cm). Sterile water was added to the bottom of the tray to make the vermiculite surface absorb water and moisten it. Use a rubber dropper to place the vernalized Arabidopsis seeds on the moist substrate surface. During the germination process, water was added in time to keep the substrate surface moist.

[0120] S2, Arabidopsis cultivation: The cultivation process is carried out in an artificial climate box, and the parameters are set as follows: the temperature time is 25°C / 16h during the day, 21°C / 8h at night, the humidity is 72%, and the light intensity is 6000lx. The above tray is placed in the incubator, and when the Arabidopsis grows to 2 weeks, the culture solution is irrigated with 25ml / flower pot, and then irrigated once a week at a fixed time, and irrigated 4 times throughout the whole process. When the Arabidopsis grows to 3 weeks, the required evaluation biological organic fertilizer preparation is irrigated, and then irrigated once a week at a fixed time, and irrigated 5 times throughout the whole process.

[0121] S3. Effect evaluation index monitoring: The root dimension is based on the root fresh weight index; the stem dimension is based on the stem length index; the flower dimension is based on the flower diameter; the leaf dimension is based on the rosette diameter; the fruit dimension is based on the fruit fresh weight index. When Arabidopsis grows to 55 days, the results of the five dimensional indicators are statistically analyzed. When statistics are performed, 8 plants with uniform growth are selected for each sample treatment; when analyzing each dimension, the maximum, minimum and difference values ​​are removed, and the average value of each dimension is used as the judgment value. According to the statistical results, the dominant action site of the evaluated fertilizer is analyzed.

[0122] As shown in the following table, among the three samples, sample 1 has a growth-promoting effect on the stems and leaves of plants, sample 2 has a growth-promoting effect on the stems and flowers of plants, and sample 3 has a growth-promoting effect on the fruits of plants.

[0123] Table 5 Effects of different biological organic fertilizers

[0124]

[0125]

[0126] Application Example 6

[0127] S1. Arabidopsis seedling cultivation: The Arabidopsis seeds used were Columbia wild type. The Arabidopsis seeds required for the test were rinsed with sterile water three times, protected from light, and placed in a 3°C refrigerator for vernalization for 72 hours. Vermiculite (1-3 mm) and quartz sand (2-5 mm) were mixed in a ratio of 2:1 and evenly placed in a flowerpot (10 cm*10 cm*10 cm). The surface was flattened and placed in a tray (40 cm*30 cm). Sterile water was added to the bottom of the tray to make the vermiculite surface absorb water and moisten it. Use a toothpick to place the vernalized Arabidopsis seeds on the moist substrate surface. During the germination process, water was added in time to keep the substrate surface moist.

[0128] S2. Arabidopsis cultivation: The cultivation process is carried out in an artificial climate chamber, and the parameters are set as follows: temperature and time are 25℃ / 16h during the day, 21℃ / 8h at night, humidity is 75%, and light intensity is 5000lx. When Arabidopsis grows to 2 weeks, water the culture solution 30ml / flower pot, and then water it once a week at a fixed time, and water it 4 times throughout the process. Place the above tray in the incubator, and when Arabidopsis grows to 3 weeks, water it with the required microbial agent for evaluation, and then water it once a week at a fixed time, and water it 5 times throughout the process.

[0129] S3. Effect evaluation index monitoring: The root dimension is based on the root length index; the stem dimension is based on the stem length index; the flower dimension is based on the number of flowers; the leaf dimension is based on the number of leaves; the fruit dimension is based on the fruit fresh weight index. When Arabidopsis grows to 56 days, the results of the five dimensional indicators are statistically analyzed. When statistics are performed, 8 plants with uniform growth are selected for each sample treatment; when analyzing each dimension, the maximum, minimum and difference values ​​are removed, and the average value of each dimension is used as the judgment value. According to the statistical results, the dominant action site of the evaluated fertilizer is analyzed.

[0130] As shown in the table below, among the three samples, sample 1 has a better overall growth-promoting effect on plants, while samples 2 and 3 have an inhibitory effect on plant growth.

[0131] Table 6 Effects of different microbial agents

[0132]

[0133]

[0134] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana, characterized in that: The following steps are involved: S1: Obtain information on the culture conditions of Arabidopsis thaliana; S2: Based on the culture condition information, setting parameter information of the artificial climate box to culture Arabidopsis thaliana; S3: Setting an evaluation index for Arabidopsis growth, wherein the evaluation index includes one or more dimensional indexes of roots, stems, flowers, leaves, and fruits; S4: Collect and compile statistics on the growth data of Arabidopsis thaliana in various dimensions, evaluate the effect of fertilizers, and determine the dominant sites of action of fertilizers on Arabidopsis thaliana.

2. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 1, characterized in that: In S1, the culture condition information includes the vernalization treatment conditions of Arabidopsis seeds, the specific substrate type required for planting, the planting density, the culture solution product required for planting, the amount of culture solution, the time of adding the culture solution, and the time of adding the fertilizer preparation required to be evaluated.

3. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 2, characterized in that: In the culture condition information, the vernalization treatment condition of the seeds is 0-5°C, and the vernalization time is 48h-96h; The soilless cultivation substrate used is 1-3 mm vermiculite and 2-5 mm quartz sand, and the vermiculite and quartz sand are evenly mixed in a ratio of 2:

1.

4. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 2, characterized in that: The planting density should provide a growth space of no less than 80mm*80mm*100mm for each Arabidopsis plant; The culture liquid product necessary for planting is 1 / 2MS culture medium, and the 1 / 2MS culture medium does not contain sucrose and agar; The amount of culture solution used for each Arabidopsis plant is 20ml-30ml; The culture solution was added at the following time: the culture solution was poured when the Arabidopsis thaliana was 2 weeks old, and then once a week at a fixed time, for a total of 4 times.

5. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 2, characterized in that: When Arabidopsis thaliana grows to 3 weeks old, water it with the required fertilizer preparation for evaluation, and then water it once a week at a fixed time, for a total of 5 times. The time interval between adding the culture solution and the required fertilizer preparation for evaluation should not be less than 2-3 days.

6. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 1, characterized in that: In S2, the parameter information of the artificial climate box includes temperature, humidity, and light conditions; The temperature of the artificial climate box was set at 25°C under light conditions and 21°C under dark conditions, the humidity was 70%-75%, and the light intensity was 5000-6000 lux.

7. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 1, characterized in that: In S3, the process of determining the evaluation index of Arabidopsis growth includes: determining root growth indicators, including one or more of root length, root fresh weight, root dry weight, and root quantity; determining stem growth indicators, including one or more of stem length, stem fresh weight, and stem dry weight; determining leaf growth indicators, including one or more of leaf number, rosette diameter, leaf fresh weight, leaf dry weight, leaf area, chlorophyll content, and photosynthesis efficiency; Determine the growth index of flowers, including one or more of the number of flowers and the diameter of flowers; Determine the growth index of the fruit, including one or more of the number of fruits, fruit size, fruit fresh weight, fruit dry weight, and fruit nutritional components.

8. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 1, characterized in that: In S3, the process of determining the evaluation index of model plant growth also includes data collection time. The data of five dimensional indicators are collected when Arabidopsis grows to 55-60 days.

9. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 1, characterized in that: In S4, the growth data of Arabidopsis thaliana in five dimensions, namely, root, stem, flower, leaf, and fruit, were comprehensively obtained within a single experimental cycle.

10. The method for evaluating fertilizer efficiency based on the model plant Arabidopsis thaliana according to claim 1, characterized in that: In S4, the type of fertilizer includes one of inorganic fertilizer, organic fertilizer, microbial fertilizer, and biostimulant.

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