Composite mildew preventive for in-situ treatment of plant rhizome specimens, composition and application

Abstract

The invention belongs to the technical field of mildew prevention and sterilization of plant specimens, and particularly relates to a composite mildew preventive for in-situ treatment of plant rhizome specimens, a composition and application. The invention firstly provides a composition for preventing mildew and sterilizing plant specimens. The composition is prepared from the following components in parts by weight: 20 to 30 parts of rhizoma coptidis extract, 10 to 30 parts of pepper extract, 5 to 10 parts of curcumine, 20 to 30 parts of fructus cnidii extract, 5 to 10 parts of sodium diacetate and 0 to 40 parts of auxiliary materials. The composition is sieved and uniformly mixed to prepare the composite mildew preventive. The composite mildew preventive is suitable for in-situ mildew prevention and removal of plant rhizome specimens, can be used for preventing mildew for a plurality of moulds for a long time, can also be used for effectively removing mildew spots and pigments on the surfaces of rhizome parts of the plant specimens, and has the advantages of being green, environmentally friendly, non-toxic and pollution-free.

Description

Technical Field

[0001] The invention belongs to the technical field of plant specimen mildew prevention and sterilization, and specifically relates to a composite mildew preventer for in-situ treatment of plant rhizome specimens, a composition and an application thereof. Background Art

[0002] Plant specimens refer to specimens of whole plants or parts of plants that can maintain their characteristics for a long time after being collected and properly processed. They are an indispensable and important resource in the fields of botanical research, teaching and cultural heritage protection. However, plant specimens are easily corroded by mold during the preservation process. In the plant specimen warehouse room in the museum, mold is the most serious biological pollution. Even in extremely harsh dry, high temperature and low temperature environments, mold spores can still survive for a long time. When the environmental conditions such as temperature and humidity are suitable, the mold spores attached to the surface of the plant specimen will germinate and grow. The high molecular organic matter proteins, fats and sugars in the plant specimens will be directly or indirectly absorbed and utilized by the mold. In addition, most molds contain colored chemical secretions. Once they break out, the surface of the plant specimens is covered with stains and plaques, which seriously changes the original appearance of the plant specimens. The acidic compounds produced by the reaction of some hydrolases secreted by molds with organic matter have a direct corrosive effect on plant specimens. Therefore, mold prevention and anti-corrosion are a key link in specimen preservation.

[0003] At present, toxic and harmful preparations such as formaldehyde and arsenic are mostly used for mildew and decay prevention of plant specimens. Such mildew inhibitors will continuously release a large amount of toxic substances into the room, often resulting in excessive concentrations of toxic substances in the indoor air, causing a large amount of unpleasant gases to accumulate in the place, causing direct harm to museum workers, visitors, school teachers and students, and polluting the environment. In addition, commonly used chemical mildew inhibitors often selectively inhibit a certain link in the metabolism of mold organisms, which can easily lead to the formation and development of microbial resistance. Many studies have shown that most molds have developed resistance to existing chemical mildew inhibitors. Moreover, the large-scale use of chemical mildew inhibitors has brought serious ecological and environmental pollution problems to the world. Therefore, it is urgent to develop safe, efficient and economical natural mildew inhibitors to replace chemical mildew inhibitors.

[0004] Plant-derived natural mildew inhibitors have become the most promising mildew inhibitor raw materials due to their broad spectrum, high efficiency, strong antibacterial properties, safety, non-toxicity, and stable performance. However, the research on plant-derived natural preservatives is still in its infancy and requires further exploration and research to improve the antiseptic and mildew-proof effects. In the existing technology,

[0005] The Chinese invention patent with publication number CN102067892A discloses an antiseptic Chinese medicine preparation and its application. The antiseptic Chinese medicine preparation comprises the following components: 38-43% of alum, 27-33% of camphor, 7-13% of boric acid, 7-13% of ginger and 7-13% of realgar, and the antiseptic Chinese medicine preparation is suitable for the preservation of animal skin specimens.

[0006] The Chinese invention patent with publication number CN112405749B discloses a natural high-efficiency antibacterial preservative, including: Sophora flavescens, Coptis chinensis, Cnidium monnieri and Cinnamomum camphora leaves. The invention uses Sophora flavescens, Coptis chinensis, Cnidium monnieri and Cinnamomum camphora leaves to work in synergy, and the resulting product has excellent antiseptic and insect repellent properties, and is suitable for wood antiseptic and antibacterial properties. Summary of the invention

[0007] In view of this, in order to overcome the deficiencies of the prior art, the present invention proposes a composite mildew-proof agent, composition and application for in-situ treatment of plant rhizome specimens. The composite mildew-proof agent is very environmentally friendly and is applied to plant specimens, especially in-situ preservation and treatment of plant rhizome specimens. It is highly efficient and non-toxic and has great promotion and application value.

[0008] One of the purposes of the present invention is to provide a composition for preventing mildew and sterilizing plant specimens.

[0009] To achieve the above object, the present invention adopts the following technical solutions:

[0010] A composition for preventing mildew and sterilizing plant specimens comprises the following components by weight: 20 to 30 parts of coptis root extract, 10 to 30 parts of prickly ash extract, 5 to 10 parts of curcumin, 20 to 30 parts of cnidium monnieri extract, 5 to 10 parts of sodium diacetate, and 0 to 40 parts of auxiliary materials.

[0011] Furthermore, the auxiliary materials include any one or more of silicon dioxide and tricalcium phosphate.

[0012] Furthermore, the berberine content in the Coptis chinensis extract is ≥85%; the zanthoxylum bungeanum content in the Zanthoxylum bungeanum extract is ≥15%; the osthole content in the Cnidium monnieri extract is ≥50%; the purity of the curcumin is ≥90%; and the purity of the sodium diacetate is ≥95%.

[0013] Preferably, the berberine content in the Coptis chinensis extract is ≥90%; the zanthoxylum bungeanum content in the Zanthoxylum bungeanum extract is ≥20%; the osthole content in the Cnidium monnieri extract is ≥60%; the curcumin purity is ≥95%; and the sodium diacetate purity is ≥96%.

[0014] Furthermore, the fineness of each component in the composition is ≥80 mesh.

[0015] Furthermore, the composition comprises the following components by weight: 20 to 30 parts of coptis root extract, 10 to 20 parts of prickly ash extract, 5 to 10 parts of curcumin, 20 to 30 parts of cnidium monnieri extract, 5 to 10 parts of sodium diacetate, and the auxiliary materials are supplemented to 100 parts; preferably, the auxiliary material is silicon dioxide.

[0016] As a preferred solution, the composition comprises the following components by weight: 25 parts of Coptis chinensis extract, 15 parts of Zanthoxylum bungeanum extract, 10 parts of curcumin, 20 parts of Cnidium monnieri extract, 10 parts of sodium diacetate, and 20 parts of silicon dioxide auxiliary material; (corresponding to Group 5 in Table 3)

[0017] Or the composition comprises the following components by weight: 25 parts of Coptis chinensis extract, 20 parts of Zanthoxylum bungeanum extract, 5 parts of curcumin, 25 parts of Cnidium monnieri extract, 5 parts of sodium diacetate, and 20 parts of silicon dioxide auxiliary material; (corresponding to Group 6 in Table 3)

[0018] Or the composition comprises the following components by weight: 30 parts of Coptis chinensis extract, 20 parts of Zanthoxylum bungeanum extract, 10 parts of curcumin, 20 parts of Cnidium monnieri extract, 10 parts of sodium diacetate, and 10 parts of silicon dioxide auxiliary material; (corresponding to Group 9 in Table 3)

[0019] Or the composition comprises the following components by weight: 20 parts of Coptis chinensis extract, 15 parts of Zanthoxylum bungeanum extract, 8 parts of curcumin, 25 parts of Cnidium monnieri extract, 5 parts of sodium diacetate, and 27 parts of silicon dioxide auxiliary material; (corresponding to Group 11 in Table 3)

[0020] Or the composition comprises the following components by weight: 20 parts of Coptis chinensis extract, 20 parts of Zanthoxylum bungeanum extract, 5 parts of curcumin, 30 parts of Cnidium monnieri extract, 8 parts of sodium diacetate, and 17 parts of silicon dioxide auxiliary material; (corresponding to Group 12 in Table 3)

[0021] Or the composition comprises the following components by weight: 25 parts of Coptis chinensis extract, 20 parts of Zanthoxylum bungeanum extract, 8 parts of curcumin, 20 parts of Cnidium monnieri extract, 8 parts of sodium diacetate, and 19 parts of silicon dioxide auxiliary material; (corresponding to Group 15 in Table 3)

[0022] Or the composition comprises the following components by weight: 30 parts of Coptis chinensis extract, 10 parts of Zanthoxylum bungeanum extract, 8 parts of curcumin, 20 parts of Cnidium monnieri extract, 10 parts of sodium diacetate, and 22 parts of silicon dioxide auxiliary material; (corresponding to Group 16 in Table 3)

[0023] Or the composition comprises the following components by weight: 30 parts of Coptis chinensis extract, 15 parts of Zanthoxylum bungeanum extract, 10 parts of curcumin, 25 parts of Cnidium monnieri extract, 8 parts of sodium diacetate, and 12 parts of silicon dioxide auxiliary material. (Corresponding to Group 17 in Table 3)

[0024] As the most preferred solution, the composition comprises the following components by weight: 30 parts of Coptis chinensis extract, 10 parts of Zanthoxylum bungeanum extract, 8 parts of curcumin, 20 parts of Cnidium monnieri extract, 10 parts of sodium diacetate, and 22 parts of silicon dioxide auxiliary material.

[0025] The second object of the present invention is to provide a composite mildew-proof agent for in-situ treatment of plant rhizome specimens.

[0026] To achieve the above object, the present invention adopts the following technical solutions:

[0027] A composite mildew preventer for in-situ treatment of plant rhizome specimens, the composite mildew preventer being prepared by sieving the composition according to claim 1 and uniformly mixing the mixture.

[0028] Preferably, each material in the composition is passed through a 80-120 mesh sieve and then uniformly mixed.

[0029] The third object of the present invention is to provide a method for preventing mold in situ plant rhizome specimens using the aforementioned composite mold inhibitor.

[0030] To achieve the above object, the present invention adopts the following technical solutions:

[0031] The method for preventing mildew of plant rhizome specimens in situ using the composite mildew preventer is to evenly apply the composite mildew preventer to the rhizome of the plant specimen.

[0032] A fourth object of the present invention is to provide an application of the aforementioned composition and / or the aforementioned composite mildew inhibitor in preventing and removing mildew from plant specimens.

[0033] To achieve the above object, the present invention adopts the following technical solutions:

[0034] Application of the aforementioned composition and / or the aforementioned composite antifungal agent in preventing mildew and rot of plant specimens while maintaining the original color of the specimens.

[0035] Furthermore, the targeted molds include Trichoderma, Penicillium, Aspergillus niger and / or Aspergillus flavus.

[0036] Furthermore, the application includes: preventing mildew and removing mildew in situ from the rhizomes of plant wax leaf specimens, and / or preventing mildew and removing mildew in situ from the rhizomes of plant part specimens.

[0037] The beneficial effects of the present invention are:

[0038] 1. The present invention proposes a composite mildew preventer for in-situ treatment of plant rhizome specimens. The main components of the composite mildew preventer include coptis chinensis extract, prickly ash extract, curcumin, cnidium monnieri extract and sodium diacetate. First, the present invention uses plant-derived extracts for mildew prevention, which has good mildew removal effect and lasting mildew prevention, is non-toxic and efficient, and has little damage to the human body and the environment. Secondly, the present invention also uses long-acting non-toxic chemical mildew prevention ingredients. At present, there is no report on the use of sodium diacetate as a specimen mildew preventive preservative. The composite mildew preventer of the present invention can make plant specimens have a strong and continuous mildew prevention effect. Furthermore, the present invention also uses plant-derived pigments at the same time, which also have a certain color preservation and color restoration effect on rhizome specimens.

[0039] 2. The present invention overcomes the shortcoming that traditional rhizome plant specimens need to be processed in a special workshop for mildew prevention and mildew removal, and the rhizome plant specimens are processed in situ, which is convenient and quick.

[0040] 3. The composite mildew preventer of the present invention does not use traditional toxic and harmful substances such as camphor, formaldehyde, arsenic, etc., and has no toxic side effects on human body and environment.

[0041] 4. The composite mildew-proof agent of the present invention is particularly suitable for plant rhizome specimens, which can keep the specimens in their original color and has good preservation, fixation, sterilization, mildew-proofing and antiseptic effects on plant rhizome specimens.

[0042] 5. The present invention can not only permanently prevent molds from various molds such as Trichoderma, Penicillium, Aspergillus niger, and Aspergillus flavus, but also effectively remove mold spots and pigments on the surface of the rhizomes of plant specimens without causing environmental pollution.

[0043] 6. The mildew inhibitor of the present invention is prepared from commercially available raw materials, the required raw materials are easy to obtain, the price is low, the operation is simple and convenient, the antiseptic effect is good, and the plant rhizome specimens after use can be preserved for a long time, and it has a good promotion and application value.

[0044] 7. The anti-mildew and antibacterial effect tests showed that the composite anti-mildew agent of the present invention had obvious inhibitory effects on Penicillium, Aspergillus niger, Trichoderma and Aspergillus flavus, and the diameter of the inhibition zone was between 20 and 40 mm. DETAILED DESCRIPTION

[0045] The technical solution of the present invention will be further described clearly and completely in conjunction with specific embodiments below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Therefore, based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work belong to the protection scope of the present invention.

[0046] The present invention proposes a composite mildew-proof agent for in-situ treatment of plant rhizome specimens. Considering that a single mildew-proof component has effective mildew-proof effect and range, the present invention composites a composite mildew-proof agent by compounding a coptis root extract, a prickly ash extract, curcumin, a cnidium monnieri extract, sodium diacetate and a silicon dioxide auxiliary material. Among them, coptis root and coptis root extract have obvious antibacterial efficacy and have significant inhibitory effects on a variety of bacteria, viruses and fungi. Prickly ash and prickly ash extract as natural antibacterial agents have obvious antibacterial effects, especially good antibacterial effects on penicillium and aspergillus niger. The main active ingredients in cnidium monnieri include osthole, prickly ash toxin, etc., which have broad-spectrum antibacterial properties, and osthole is the monomer with the strongest antibacterial activity, with antibacterial, antiviral and anti-inflammatory pharmacological effects, and these ingredients have significant inhibitory effects on a variety of bacteria and fungi. Curcumin is a good natural pigment preservative, integrating coloring, preservation and sterilization. Sodium diacetate mainly interferes with the interaction of enzymes by effectively penetrating into the cell wall of molds, inhibiting the production of molds, thereby achieving functions such as efficient mildew prevention and antiseptic. Sodium diacetate has a better inhibitory effect on Aspergillus niger, Rhizopus niger, and Aspergillus flavus than potassium sorbate. In short, the composite mildew inhibitor of the present invention is prepared by a certain proportion of several mildew-proof components with synergistic effects, and its synergistic effect overcomes the limitations of a single mildew inhibitor in mildew-proof efficacy, and expands the antibacterial efficacy and range. The composite mildew inhibitor of the present invention has the advantages of a wide antibacterial spectrum, good mildew-proof effect, little influence by other factors, small dosage, low acidity requirement, non-corrosiveness and irritation.

[0047] The raw materials used in the present invention can all be purchased through regular commercial channels. In the embodiments of the present invention, the coptis root extract was purchased from Shaanxi Jiuzhou Kangyuan Biotechnology Co., Ltd.; the prickly ash extract was purchased from Shaanxi Botao Biological Co., Ltd.; the curcumin was purchased from Handan Tianxu Natural Pigment Co., Ltd.; the cnidium monnieri extract was purchased from Shaanxi Botao Biological Co., Ltd.; sodium diacetate was purchased from Qingdao Dawei Bioengineering Co., Ltd.; and silicon dioxide was purchased from Zhenhan New Materials (Suzhou) Co., Ltd.

[0048] In the embodiment of the present invention, the berberine content in the Coptis chinensis extract used is ≥90%; the scutellarin content in the Zanthoxylum bungeanum extract is ≥20%; the purity of curcumin is ≥95%; the osthole content in the Cnidium monnieri extract is ≥60%; and the purity of sodium diacetate is ≥96%.

[0049] In the embodiment of the present invention, the method for detecting the anti-mildew effect is as follows:

[0050] 1. Selected test species: Penicillium, Aspergillus niger, Trichoderma, Aspergillus flavus (all isolated from plant specimens);

[0051] 2. Detection method: plate inhibition zone method (using nutrient agar plate);

[0052] 3. Detection temperature: 25℃±2℃;

[0053] 4. Detection time: 48 hours;

[0054] 5. Test results: measured with a caliper.

[0055] In the embodiment of the present invention, the antibacterial effect testing method is as follows:

[0056] 1. Selected strains: Trichoderma, Penicillium, Aspergillus niger, Aspergillus flavus (all isolated from plant specimens); 2. Detection method: plate inhibition zone method (using nutrient agar plate);

[0057] 3. Detection temperature: 30℃±1℃;

[0058] 4. Detection time: 24 hours;

[0059] 5. Test results: measured with a caliper.

[0060] Example 1. Antifungal agent design

[0061] (1) Antibacterial effect of antifungal agent combination on different molds

[0062] First, the antibacterial effects of different extract combinations of mildew inhibitors on different molds were investigated. The results are shown in Table 1.

[0063] Table 1. Antibacterial effect of antifungal agent combination on different molds (mm)

[0064]

[0065]

[0066] Note: Different letters indicate significant differences at the 5% level.

[0067] (2) Orthogonal design

[0068] Table 2. Orthogonal factors and levels (weight parts)

[0069]

[0070] Table 3. Orthogonal design table and results

[0071]

[0072]

[0073] As shown in Table 3, the inhibition zones of the composite mildew inhibitors of different combinations are all between 20 and 40 mm, and the composite mildew inhibitor composed of 30 parts of coptis chinensis extract, 10 parts of pepper extract, 8 parts of curcumin, 20 parts of cnidium monnieri extract, 10 parts of sodium diacetate, and 22 parts of auxiliary materials has the largest inhibition zone, reaching 39.88 mm.

[0074] Example 2

[0075] A composite mildew preventer for in-situ treatment of plant rhizome specimens, comprising the following components by weight:

[0076] 30 parts of Coptis chinensis extract (berberine ≥ 90%), 20 parts of Zanthoxylum bungeanum extract (Zanthoxylum bungeanum ≥ 20%), 10 parts of curcumin (purity ≥ 95%), 25 parts of Cnidium monnieri extract (Cnidium monnieri ≥ 60%), 10 parts of sodium diacetate (purity ≥ 96%), and 5 parts of silicon dioxide auxiliary material.

[0077] The above materials were sieved through a 100-mesh sieve and then uniformly mixed to obtain a composite mildew inhibitor. The anti-mildew effect test showed that the composite mildew inhibitor had significant inhibitory effects on Penicillium, Aspergillus niger, Trichoderma and Aspergillus flavus, and the diameters of the inhibition zones were all ≥38 mm.

[0078] Example 3

[0079] A composite mildew preventer for in-situ treatment of plant rhizome specimens, comprising the following components by weight:

[0080] 20 parts of Coptis chinensis extract (berberine ≥ 90%), 30 parts of Zanthoxylum bungeanum extract (Zanthoxylum bungeanum ≥ 20%), 5 parts of curcumin (purity ≥ 95%), 30 parts of Cnidium monnieri extract (Cnidium monnieri ≥ 60%), 5 parts of sodium diacetate (purity ≥ 96%), and 10 parts of tricalcium phosphate auxiliary material.

[0081] The above materials were passed through an 80-mesh sieve and then uniformly mixed to obtain a composite mildew inhibitor. The anti-mildew effect test showed that the composite mildew inhibitor had obvious inhibitory effects on Penicillium, Aspergillus niger, Trichoderma and Aspergillus flavus, and the diameter of the inhibition zone was ≥38 mm.

[0082] Example 4

[0083] A composite mildew preventer for in-situ treatment of plant rhizome specimens, comprising the following components by weight:

[0084] 25 parts of Coptis chinensis extract (berberine ≥ 90%), 25 parts of Zanthoxylum bungeanum extract (Zanthoxylum bungeanum ≥ 20%), 5 parts of curcumin (purity ≥ 95%), 25 parts of Cnidium monnieri extract (osmitoyl ester ≥ 60%), 5 parts of sodium diacetate (purity ≥ 96%), and 15 parts of silicon dioxide auxiliary material.

[0085] The above materials were passed through a 120-mesh sieve and then uniformly mixed to obtain a composite mildew preventer. The anti-mildew effect test showed that the composite mildew preventer had obvious inhibitory effects on Penicillium, Aspergillus niger, Trichoderma and Aspergillus flavus, and the diameter of the inhibition zone was ≥38 mm.

[0086] Example 5

[0087] A composite mildew preventer for in-situ treatment of plant rhizome specimens, comprising the following components by weight:

[0088] 30 parts of Coptis chinensis extract (berberine ≥ 90%), 15 parts of Zanthoxylum bungeanum extract (Zanthoxylum bungeanum ≥ 20%), 8 parts of curcumin (purity ≥ 95%), 25 parts of Cnidium monnieri extract (osmatolin ≥ 60%), 10 parts of sodium diacetate (purity ≥ 96%), and 12 parts of silicon dioxide auxiliary material.

[0089] The above materials were passed through a 120-mesh sieve and then uniformly mixed to obtain a composite mildew preventer. The anti-mildew effect test showed that the composite mildew preventer had obvious inhibitory effects on Penicillium, Aspergillus niger, Trichoderma and Aspergillus flavus, and the diameter of the inhibition zone was ≥38 mm.

[0090] Example 6. In situ mildew prevention of plant rhizomes

[0091] (1) Mildew prevention of rhizomes of wax leaf specimens

[0092] The composite mildew inhibitor with the best antibacterial effect prepared in Example 1 (Group 16 in Table 3) was evenly applied to the rhizome of the wax leaf specimens with a brush or other tools, and the specimens without application were used as controls. The specimens were placed in a constant temperature box at 30°C and 85% humidity and observed after 5 days.

[0093] After 5 days, it was found that the wax leaf specimens coated with the composite mildew inhibitor did not have any bacterial plaques, while the uncoated controls all had bacterial plaques.

[0094] (2) Mold prevention for rhizome plant specimens

[0095] An appropriate amount of the composite mildew preventer prepared after optimization (Group 16 in Table 3) was directly placed in a sealed glass bottle containing rhizome plant specimens, while a sealed glass bottle without the composite mildew preventer was used as a control. The bottle was placed in a constant temperature box at 30°C and 85% humidity and observed after 5 days.

[0096] After 5 days, it was found that there was no bacterial plaque in the plant specimen bottle that had been placed in the composite mildew inhibitor, while a small amount of bacterial plaque appeared in the control bottle that had not been placed in it.

[0097] Example 7. In situ mildew removal of plant rhizome specimens

[0098] (1) Mold removal of rhizomes of wax leaf specimens

[0099] On the rhizome part of the wax leaf specimen with mold, the composite mildew inhibitor prepared after optimization (Group 16 in Table 3) was evenly applied with a brush or other tools and placed in a constant temperature box. A commercial mildew remover was used as a control, and it was placed in a constant temperature box with a temperature of 30°C and a humidity of 85%, and observed after 5 days.

[0100] After 5 days of observation, it was found that the fungal plaques on the wax leaf specimens coated with the composite mildew inhibitor and the commercial mildew remover disappeared, but the color of the rhizomes of the wax leaf specimens coated with the composite mildew inhibitor remained original.

[0101] (2) Mold removal of rhizome plant specimens

[0102] An appropriate amount of the prepared composite mildew inhibitor (Group 16 in Table 3) was placed in a sealed glass bottle containing rhizome plant specimens with mold, and the bottle was placed in a constant temperature box. A commercial mildew remover was used as a control, and the bottle was placed in a constant temperature box at 30°C and 85% humidity, and observed after 5 days.

[0103] After 5 days, it was found that there were no bacterial plaques in the plant specimen bottles placed in the composite mildew inhibitor and the commercial mildew remover, but the color of the rhizomes of the wax leaf specimens coated with the composite mildew inhibitor remained original.

Claims

1. A composition for preventing mildew and sterilizing plant specimens, characterized in that: The composition comprises the following components by weight: 20 to 30 parts of coptis root extract, 10 to 30 parts of prickly ash extract, 5 to 10 parts of curcumin, 20 to 30 parts of cnidium monnieri extract, 5 to 10 parts of sodium diacetate and 0 to 40 parts of auxiliary materials.

2. The composition according to claim 1, characterized in that The auxiliary materials include any one or more of silicon dioxide and tricalcium phosphate.

3. The composition according to claim 1, characterized in that The berberine content in the coptis extract is ≥85%; the scutellarin content in the zanthoxylum extract is ≥15%; the osthole content in the cnidium monnieri extract is ≥50%; the curcumin purity is ≥90%; and the sodium diacetate purity is ≥95%.

4. The composition according to claim 1, characterized in that The composition comprises the following components by weight: 20 to 30 parts of coptis root extract, 10 to 20 parts of prickly ash extract, 5 to 10 parts of curcumin, 20 to 30 parts of cnidium monnieri extract, 5 to 10 parts of sodium diacetate, and auxiliary materials are supplemented to 100 parts.

5. The composition according to claim 1, characterized in that The composition comprises the following components by weight: 30 parts of coptis root extract, 10 parts of prickly ash extract, 8 parts of curcumin, 20 parts of cnidium monnieri extract, 10 parts of sodium diacetate, and 22 parts of auxiliary materials; the auxiliary materials are silicon dioxide or tricalcium phosphate.

6. A composite mildew-proofing agent for in-situ treatment of plant rhizome specimens, characterized in that: The composite mildew preventer is prepared by sieving the composition according to claim 1 and uniformly mixing them.

7. A method for preventing mold in situ plant rhizomes using the composite mold inhibitor according to claim 6, characterized in that: The composite mildew preventer is evenly applied to the root and stem parts of the plant specimen.

8. Use of the composition according to any one of claims 1 to 5 and / or the composite mildew inhibitor according to claim 6 in preventing and removing mildew from plant specimens while maintaining the original color of the specimens.

9. The use according to claim 8, characterized in that: The targeted molds include Trichoderma, Penicillium, Aspergillus niger and / or Aspergillus flavus.

10. The use according to claim 8, characterized in that: The application includes: preventing mildew and removing mildew in situ from the rhizomes of plant wax leaf specimens, and / or preventing mildew and removing mildew in situ from the rhizomes of plant part specimens.

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