Preparation method, application and product of liver-protecting and hangover-alleviating abalone extract

Abstract

The invention discloses a preparation method of an abalone extract capable of protecting the liver and dispelling the effects of alcohol, application of the abalone extract and a product. The preparation method comprises the following steps: uniformly mixing abalone powder with low-concentration ethanol to obtain a mixture, and performing enzymolysis on the mixture to obtain abalone enzymatic hydrolysate; separating and purifying the obtained abalone enzymatic hydrolysate to obtain trapped fluid; and concentrating the obtained trapped fluid to obtain a concentrated solution. According to the method, a low-concentration ethanol enzymolysis technology is creatively used, and the abalone powder is subjected to enzymolysis in a low-concentration ethanol solution by utilizing alcohol-resistant neutral protease, so that effective active ingredients can be released, and impurities can be removed, thereby facilitating enrichment and purification of the active ingredients. The abalone extract obtained by the preparation method disclosed by the invention can be directly eaten and does not need to be treated again, and meanwhile, the abalone extract is safer due to low-concentration ethanol and no need of adding an acid-base reagent to adjust the pH value in a working section.

Description

Technical Field

[0001] The invention relates to the field of biological extracts, and in particular to a preparation method of a liver-protecting and alcohol-relieving abalone extract and its application and product. Background Art

[0002] After the human body ingests ethanol, about 25% is first absorbed by the gastric mucosal cells and metabolized by the alcohol dehydrogenase in the gastric mucosa. The remaining ethanol quickly enters the intestine and is absorbed. It then flows through the liver repeatedly with the blood and is metabolized by the alcohol dehydrogenase system in the liver to generate carbohydrates, water and a small amount of energy. In the end, less than 2% of the ethanol is excreted through urine, lung breathing or skin epidermis. Therefore, increasing the ethanol metabolism rate can reduce the burden on the liver after drinking, thereby protecting the liver.

[0003] Abalone, also known as mirror fish, nine-hole snail, eye-catching fish, and general cap, has more than 100 species of abalone in the world. It is widely loved for its rich nutritional value and delicious taste. It is known as "a mouthful of abalone is a mouthful of gold". According to analysis, the edible part of abalone contains 24% protein, 0.44% fat, and contains a variety of vitamins and some special trace components. It has high therapeutic effects and medicinal value, such as supplementing the protein and other nutrients needed by the human body. The active substances extracted from it also have the effects of sobering up, protecting the liver, anti-oxidation, and enhancing immunity. The current methods for extracting active substances from abalone are as follows:

[0004] The invention patent with application number 201510626266.1 discloses a method for preparing abalone viscera antioxidant: using abalone viscera as raw material, abalone viscera antioxidant peptide powder is obtained through defatting, protein removal, hot water extraction, high-speed centrifugation and dialysis, and then mixed with sodium alginate and added to calcium chloride, and finally vacuum dried to obtain a safe, non-toxic, stable, and strong antioxidant activity abalone viscera protein peptide antioxidant. However, the production process of this method is complicated, and the product is mainly used for meat preservation and is not suitable for direct consumption as food.

[0005] The invention patent with application number 202010299549.0 discloses an abalone viscera extract and its application. The method comprises homogenizing, freeze-drying, ultrasound-assisted extraction, constant temperature extraction, centrifugation, reduced pressure concentration, and freeze-drying the abalone viscera to obtain an abalone viscera extract that protects the reproductive system of female mice. However, this method requires two freeze-drying steps, which is costly and not suitable for large-scale industrial production.

[0006] The invention patent with application number 202010740272.0 discloses an industrial abalone peptide extraction method, which is to make abalone into minced meat, add alkali to defat, wash, adjust pH, extract, separate oil, enzymatically hydrolyze, inactivate enzymes, deodorize and decolorize, nanofilter, concentrate, and spray dry to obtain abalone peptides. Among the abalone peptides obtained by this method, oligopeptides with a molecular weight of less than 1000Da account for 86%. Although the degree of enzymatic hydrolysis is high, the efficacy of the obtained small molecule abalone peptides has not been disclosed.

[0007] The invention patent with application number 202310677452.2 discloses a uric acid-lowering abalone visceral polypeptide and its preparation method and application. The method uses abalone viscera as raw material, and obtains a uric acid-lowering abalone visceral polypeptide through homogenization, enzymatic hydrolysis under weak alkaline conditions, enzymatic hydrolysis under acidic conditions, centrifugation, ultrafiltration, and chromatography, and its amino acid sequence is LES. This method requires the pH to be adjusted to 1.0-3.0 during enzymatic hydrolysis under acidic conditions, which is not suitable for industrial production.

[0008] The existing technology for extracting abalone active substances is relatively complex and costly, and the application of abalone extracts is limited. Therefore, there is an urgent need for a low-cost, simple process preparation method for abalone extracts, and further exploration of the application of abalone extracts. Summary of the invention

[0009] The purpose of the present invention is to provide a simple, safe and industrially producible method for preparing an abalone extract having liver-protecting and alcohol-resolving effects and a product.

[0010] To achieve the above object, the first aspect of the present invention provides a method for preparing a liver-protecting and hangover-relieving abalone extract, comprising:

[0011] S1, abalone powder and low concentration of ethanol are mixed to obtain mixture, and the mixture is hydrolyzed to obtain abalone hydrolyzate;

[0012] S2, separating and purifying the abalone hydrolyzate obtained in S1 to obtain a retentate;

[0013] S3, concentrating the retentate obtained in S2 to obtain a concentrated solution.

[0014] The low-concentration ethanol described in the present invention is ethanol with a volume percentage of less than 40%, and the ethanol concentration is preferably 20-30% by volume.

[0015] Furthermore, the concentrated solution obtained in step S3 is further spray-dried to obtain abalone peptide powder.

[0016] Furthermore, the enzymolysis in step S1 is performed by a neutral protease that is active in low-concentration ethanol; preferably, the low-concentration ethanol in step S1 is food-grade ethanol with a volume percentage of 20-30%. The neutral protease maintains 50%-70% of the original enzyme activity in 20%-30% ethanol, and the enzymolysis of abalone powder can release effective active ingredients. Ethanol can also be used to precipitate some soluble impurities so that impurities can be removed during separation and purification, thereby enriching and purifying active ingredients, so as to achieve the purpose of reducing steps.

[0017] Furthermore, in step S1, the mass ratio of abalone powder: low concentration ethanol: neutral protease is 1:3-5:0.005-0.01.

[0018] Furthermore, the abalone powder in step S1 is obtained by pre-treating abalone, drying and crushing the edible part of the abalone;

[0019] Preferably, the pretreatment is to remove the shell of the live abalone, take out the meat and viscera, and further wash, blanch, cool and drain to obtain the edible part;

[0020] Preferably, the drying temperature is 45-55°C;

[0021] Preferably, the edible part of the abalone is dried until it can be crushed into powder; more preferably, the drying time is 40-48 hours.

[0022] Blanching can kill some spoilage bacteria and endogenous enzymes, and washing with room temperature water can quickly cool down the temperature, preventing the center temperature from being high during drying, thereby preventing the raw materials from deteriorating. The edible part of the abalone is then dried at 45-55°C. Compared with sun exposure, it can speed up the drying rate and prevent the edible part of the abalone from deteriorating during long-term sun exposure. Drying at a lower temperature can also prevent the edible part of the abalone from maturation. After drying the edible part of the abalone, crushing it into abalone powder can allow the enzyme to fully contact with the protein, improve the enzymatic efficiency and product yield.

[0023] Furthermore, the temperature of enzymolysis in step S1 is 50-55° C.; preferably, the time of enzymolysis in step S1 is 1.5 h-2.5 h.

[0024] Furthermore, in step S2, the abalone hydrolysate is centrifuged to obtain a supernatant, the supernatant is coarsely filtered to obtain a filtrate, and the filtrate is ultrafiltered to obtain a retentate containing the active ingredient;

[0025] Preferably, the centrifugation is performed at 10000-12000 rpm for 25-30 min;

[0026] Preferably, the coarse filtration is performed using a filter cloth with a mesh size of 150-250;

[0027] Preferably, the ultrafiltration is to filter the filtrate using an ultrafiltration membrane separation device with a molecular weight cutoff of 3000Da.

[0028] In vitro experiments have found that the activation rate of abalone hydrolysate components with a molecular weight of more than 3000Da for alcohol dehydrogenase (ADH) is 5.15 times that of abalone hydrolysate components with a molecular weight of less than 3000Da. Therefore, in production, the precipitation and macromolecular substances are first removed by centrifugation and coarse filtration to make the ultrafiltration membrane separation equipment less likely to be blocked, slow down the rate of membrane equipment flux decline, and thus improve the separation and purification efficiency; then the filtrate is ultrafiltered to remove substances less than 3000Da in the filtrate to obtain a retentate with a high content of active ingredients.

[0029] Furthermore, the concentration of the concentrated solution is 15-20° Bx; preferably, the retentate in step S3 is concentrated at 50-60° C. to obtain the concentrated solution.

[0030] The abalone extract prepared by the preparation method provided in the first aspect of the present invention has the purpose of protecting the liver and relieving alcohol.

[0031] The second aspect of the present invention provides a product containing abalone extract for protecting liver and relieving alcohol, wherein the abalone extract obtained by the preparation method provided in the first aspect of the present invention is prepared into one or more of capsules, tablets, granules, oral liquids or beverages.

[0032] The preparation method proposed in the present invention prepares abalone powder from pretreated abalone and mixes it with low-concentration ethanol, and enzymolyzes the abalone powder with neutral protease at natural pH, thereby reducing the introduction of ash and making the purity of the effective ingredients in the product higher.

[0033] The ultrafiltration membrane retentate obtained by enzymolysis of abalone powder with low concentration ethanol has higher liver protection and alcohol sobering activity than the ultrafiltration membrane retentate obtained by enzymolysis of abalone powder in water. Therefore, when extracting the abalone extract with liver protection and alcohol sobering effect, the present invention uses a neutral protease that maintains 50%-70% of the original activity in ethanol to enzymolyze the abalone powder to obtain an enzymolysis solution with higher effective active ingredients, and then separates and purifies to remove insoluble impurities and macromolecular substances, and specifically obtains a retentate with higher active ingredient content. The abalone extract obtained by the preparation method of the present invention can be eaten directly without reprocessing, which reduces costs and saves work sections. At the same time, the low concentration of ethanol and the absence of adding acid-base reagents to adjust pH in the work section also make the preparation method proposed in the present invention safer.

[0034] The concentrate can be further spray-dried to prepare powdered abalone extract for storage, transportation and further processing. At the same time, in order to better adapt to the consumer market, the abalone extract containing active ingredients can be prepared into capsules, tablets, granules, oral liquids or beverages to cater to the preferences of different consumers.

[0035] The present invention creatively uses low-concentration ethanol enzymatic hydrolysis technology, and utilizes alcohol-resistant neutral protease to enzymatically hydrolyze abalone powder in a low-concentration ethanol solution, which can not only release effective active ingredients, but also utilize ethanol to precipitate some soluble impurities, making it easier to enrich the active ingredients through subsequent separation and purification, thereby specifically obtaining an abalone extract rich in active ingredients.

[0036] The abalone extract prepared by the present invention can increase the enzyme activity of ethanol metabolism-related enzymes and accelerate the metabolism of ethanol in the liver, thereby achieving the effect of protecting the liver and sobering up. Volunteer experiments have shown that after taking 1g of abalone extract and then drinking 40g of liquor with an alcohol concentration of 40%, the time for the blood ethanol concentration to return to 0 is 16.7% earlier than the time without taking abalone extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is a graph showing the experimental results of the activation rate of ADH by different components in Example 1 of the present invention;

[0038] Figure 2 It is a graph showing the experimental results of the activation rate of ADH by the retentate in Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 of the present invention;

[0039] Figure 3 This is a graph showing the experimental results of the effect of the product prepared in Example 1 of the present invention on the hangover sobering rate. DETAILED DESCRIPTION

[0040] Embodiments of the present invention are described in detail below, and the examples of the embodiments are intended to be used to explain the present invention, and should not be construed as limiting the present invention. In the embodiments, if specific techniques or conditions are not indicated, the techniques or conditions described in the literature in this area or the product specifications are carried out. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products that can be obtained commercially. In the following embodiments, if not clearly stated, "%" refers to weight percentage.

[0041] 1. The neutral protease used in the present invention is a neutral protease produced by Nanning Pangbo Bioengineering Co., Ltd., and the enzyme activity is 150000U / g.

[0042] 2. The abalone used in the embodiment of the present invention is any one of Haliotis discus hannai, Haliotis discus, Haliotis discus, Haliotis discus, and Haliotis discus that are fresh and have no obvious fishy smell.

[0043] 3. The preparation method of the abalone extract for protecting liver and relieving alcohol in the present invention is as follows:

[0044] S1, abalone powder and low concentration of ethanol are mixed to obtain mixture, and the mixture is hydrolyzed to obtain abalone hydrolyzate;

[0045] S2, separating and purifying the abalone hydrolyzate obtained in S1 to obtain a retentate;

[0046] S3, concentrating the retentate obtained in S2 to obtain a concentrated solution.

[0047] Preferably, the concentrated solution obtained in step S3 is further spray-dried to obtain abalone peptide powder.

[0048] Preferably, the enzymatic hydrolysis in step S1 is performed by a neutral protease that is active in low concentration ethanol; more preferably, the ethanol in step S1 is food grade ethanol with a volume percentage of 20-30%.

[0049] Preferably, in step S1, the mass ratio of abalone powder: ethanol: neutral protease is 1: 3-5: 0.005-0.01.

[0050] Preferably, the abalone powder in step S1 is obtained by pre-treating abalone, drying and crushing the edible part of the abalone;

[0051] More preferably, the pretreatment is to shell the live abalone, take out the meat and viscera, and further wash, blanch, cool and drain to obtain the edible part;

[0052] More preferably, the drying temperature is 45-55°C;

[0053] More preferably, the edible part of the abalone is dried until it can be crushed into powder; more preferably, the drying time is 40-48 hours.

[0054] Preferably, the temperature of enzymolysis in step S1 is 50-55° C.; more preferably, the time of enzymolysis in step S1 is 1.5 h-2.5 h.

[0055] Preferably, in step S2, the abalone hydrolysate is centrifuged to obtain a supernatant, the supernatant is coarsely filtered to obtain a filtrate, and the filtrate is ultrafiltered to obtain a retentate containing the active ingredient;

[0056] More preferably, the centrifugation is performed at 10000-12000 rpm for 25-30 min;

[0057] More preferably, the coarse filtration is performed using a filter cloth with a mesh size of 150-250;

[0058] More preferably, the ultrafiltration is to filter the filtrate using an ultrafiltration membrane separation device with a molecular weight cutoff of 3000Da.

[0059] Preferably, the retentate in step S3 is concentrated at 50-60°C to obtain a concentrated solution; more preferably, the concentration of the concentrated solution is 15-20°Bx.

[0060] Preferably, the concentrated solution obtained in step S3 is further spray-dried to obtain abalone extract powder.

[0061] Embodiment 1:

[0062] Take 50 kg of fresh abalone raw material, remove the shell, take out the meat and viscera, wash once with normal temperature water, blanch with boiling water for 5 minutes, wash once with normal temperature water, drain, dry at 50 ° C for 48 hours, crush with an ultrafine grinder and sieve to obtain abalone powder with a particle size of less than 120 mesh.

[0063] S1, add 25% food-grade ethanol aqueous solution with a volume percentage of 5 times its weight to abalone powder, after mixing, warm up to 55 DEG C, add 500g neutral protease, enzymolysis 2h under natural pH condition, constantly stir during enzymolysis, enzymolysis ends, and obtains abalone enzymolysis solution.

[0064] S2, abalone enzymolysis liquid is centrifuged at 12000rpm for 30min, supernatant is taken, filtrate is obtained after coarse filtration with 200 mesh filter cloth, and then filtrate is ultrafiltered by ultrafiltration membrane separation equipment with molecular weight cut-off being 3000Da to obtain trapped liquid.

[0065] S3. The retentate is passed into a vacuum concentration tank and concentrated under reduced pressure to 15°Bx at 60°C to obtain a concentrated solution.

[0066] The concentrated solution is spray-dried and powdered at 180° C., and a product containing the liver-protecting and hangover-relieving abalone extract is obtained after packaging.

[0067] Test Example 1:

[0068] Activation experiment of alcohol dehydrogenase by components of different molecular weights:

[0069] The activation rate of alcohol dehydrogenase (ADH) by the filtrate (obtained by coarse filtration through filter cloth), the retentate (component with a molecular weight of >3000 Da obtained by ultrafiltration) and the permeate (component with a molecular weight of <3000 Da obtained by ultrafiltration) in Example 1 was determined using the alcohol dehydrogenase (ADH) activity assay kit of Nanjing Jiancheng Bioengineering Institute. The test method was carried out according to the instructions. The results are shown in FIG. Figure 1 shown.

[0070] The analysis results show that the activation rate of ADH by different components is 3.61% for the permeate and 18.63% for the retentate. The activation rate of ADH by the retentate is 5.16 times that of the permeate. Therefore, the retentate is selected for subsequent treatment in the production process to increase the content of effective ingredients in the liver protection and alcohol detoxification products.

[0071] Comparative Example 1:

[0072] Take 50 kg of fresh abalone raw material, remove the shell, take out the meat and viscera, wash once with normal temperature water, blanch with boiling water for 5 minutes, wash once with normal temperature water, drain, dry at 50 ° C for 48 hours, crush with an ultrafine grinder and sieve to obtain abalone powder with a particle size of less than 120 mesh.

[0073] S1, in abalone powder, add 5 times its weight of water, after mixing, be warming up to 55 DEG C, add the neutral protease of 500g, enzymolysis 2h under natural pH condition, constantly stir during enzymolysis, enzymolysis terminates, and obtains abalone enzymolysis solution.

[0074] S2, abalone enzymolysis liquid is centrifuged at 12000rpm for 30min, supernatant is taken, filtrate is obtained after coarse filtration with 200 mesh filter cloth, and then filtrate is ultrafiltered by ultrafiltration membrane separation equipment with molecular weight cut-off being 3000Da to obtain trapped liquid.

[0075] S3. The retentate is passed into a vacuum concentration tank and concentrated under reduced pressure to 15°Bx at 60°C to obtain a concentrated solution.

[0076] The concentrated solution is spray-dried and powdered at 180° C., and a product containing the liver-protecting and hangover-relieving abalone extract is obtained after packaging.

[0077] Comparative Example 2:

[0078] Take 50 kg of fresh abalone raw material, remove the shell, take out the meat and viscera, wash once with normal temperature water, blanch with boiling water for 5 minutes, wash once with normal temperature water, drain, dry at 50 ° C for 48 hours, crush with an ultrafine grinder and sieve to obtain abalone powder with a particle size of less than 120 mesh.

[0079] S1, add 15% food-grade ethanol aqueous solution with a volume percentage of 5 times its weight to abalone powder, after mixing, warm up to 55 DEG C, add 500g neutral protease, enzymolysis 2h under natural pH condition, constantly stir during enzymolysis, enzymolysis ends, and obtains abalone enzymolysis solution.

[0080] S2, abalone enzymolysis liquid is centrifuged at 12000rpm for 30min, supernatant is taken, filtrate is obtained after coarse filtration with 200 mesh filter cloth, and then filtrate is ultrafiltered by ultrafiltration membrane separation equipment with molecular weight cut-off being 3000Da to obtain trapped liquid.

[0081] S3. The retentate is passed into a vacuum concentration tank and concentrated under reduced pressure to 15°Bx at 60°C to obtain a concentrated solution.

[0082] The concentrated solution is spray-dried and powdered at 180° C., and a product containing the liver-protecting and hangover-relieving abalone extract is obtained after packaging.

[0083] Comparative Example 3:

[0084] Take 50 kg of fresh abalone raw material, remove the shell, take out the meat and viscera, wash once with normal temperature water, blanch with boiling water for 5 minutes, wash once with normal temperature water, drain, dry at 50 ° C for 48 hours, crush with an ultrafine grinder and sieve to obtain abalone powder with a particle size of less than 120 mesh.

[0085] S1, add 35% food-grade ethanol aqueous solution with a volume percentage of 5 times its weight to abalone powder, after mixing, warm up to 55 DEG C, add 500g neutral protease, enzymolysis 2h under natural pH condition, constantly stir during enzymolysis, enzymolysis ends, and obtains abalone enzymolysis solution.

[0086] S2, abalone enzymolysis liquid is centrifuged at 12000rpm for 30min, supernatant is taken, filtrate is obtained after coarse filtration with 200 mesh filter cloth, and then filtrate is ultrafiltered by ultrafiltration membrane separation equipment with molecular weight cut-off being 3000Da to obtain trapped liquid.

[0087] S3. The retentate is passed into a vacuum concentration tank and concentrated under reduced pressure to 15°Bx at 60°C to obtain a concentrated solution.

[0088] The concentrated solution was spray dried at 180°C to make powder, and then packaged to obtain a product containing abalone extract for protecting liver and relieving alcohol. Test Example 2:

[0089] (1) Flux test during separation and purification using ultrafiltration membrane separation equipment:

[0090] The filtrate obtained by coarse filtration after centrifugation was passed into an ultrafiltration membrane separation device with a molecular weight cutoff of 3000Da. The inlet and outlet pressures of the device were controlled to be 2Mpa, and the flux of the permeate within 1 hour was measured.

[0091] Table 1 Changes in membrane flux during ultrafiltration

[0092]

[0093] From the above results, it can be seen that the membrane flux of Comparative Example 1 decreases faster than that of Example 1, the membrane flux changes of Comparative Example 2 and Example 1 are similar, and the membrane flux of Comparative Example 3 decreases rapidly and is obviously seriously blocked. It shows that enzymatic hydrolysis of abalone in an ethanol solution of suitable concentration is helpful to remove impurities, slow down the decline of membrane flux caused by impurities, and thus accelerate the efficiency of ultrafiltration membrane separation and purification.

[0094] (2) Alcohol dehydrogenase activation experiment:

[0095] The activation rate of alcohol dehydrogenase (ADH) by the ultrafiltration membrane retentate in Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 was determined using the alcohol dehydrogenase (ADH) activity assay kit of Nanjing Jiancheng Bioengineering Institute. The test method was carried out according to the instructions. The results are as follows: Figure 2 shown.

[0096] The results show that the activation rate of ADH by the retentate in Example 1 is 18.63%, that of Comparative Example 1 is 3.53%, that of Comparative Example 2 is 9.27%, and that of Comparative Example 3 is 1.72%. Therefore, using an appropriate concentration of ethanol to enzymatically hydrolyze abalone powder during the production process can release more active substances, thereby increasing the content of effective ingredients in the liver protection and alcohol sobering products.

[0097] (3) Verification of in vivo hangover-relief effect:

[0098] Ten adult volunteers were recruited to test the effect of the product containing the liver-protecting and hangover-relieving abalone extract prepared in Example 1 on the hangover-relieving rate.

[0099] The volunteers were aged between 24 and 45, including 5 males and 5 females. In the blank group, each volunteer drank 40g of a commercially available liquor with an alcohol concentration of 40% within 1 minute at 15:00 on the first day. The blood alcohol concentration was measured once every 15 minutes using a breath alcohol content tester until the blood alcohol concentration reached 0mg / 100mL. The results were as follows: Figure 3 In the blank group, it took 180 minutes for the ethanol concentration in the volunteers' blood to drop from 25 mg / 100 mL to 0 mg / 100 mL.

[0100] Three days later, each volunteer took 1g of the product containing the abalone extract for protecting liver and relieving alcohol prepared in Example 1 at 14:30, and drank 40g of a commercially available liquor with an alcohol concentration of 40% within 1min at 15:00. The blood ethanol concentration was measured once every 15min using a breath alcohol content tester as the experimental group data until the blood ethanol concentration reached 0mg / 100mL. The results are as follows: Figure 3 In the experimental group, the initial ethanol concentration in the volunteers' blood dropped from 25 mg / 100 mL to 20 mg / 100 mL, and it only took 150 minutes for the ethanol concentration in the blood to drop to 0 mg / 100 mL.

[0101] After taking the liver-protecting and hangover-relieving product, the hangover-relieving time of drinking is 30 minutes earlier than that without taking it, and the hangover-relieving rate is increased by 16.7%. At the same time after drinking, the ethanol concentration in the blood is lower than that without taking the liver-protecting and hangover-relieving product. Therefore, it is speculated that the liver-protecting and hangover-relieving product prepared by the present invention also has the effect of slowing down the time of drunkenness. Since most of the ingested ethanol is metabolized in the liver, the abalone extract prepared by the present invention can increase the activity of ADH, accelerate ethanol metabolism, and reduce the burden on the liver, so it has the effect of protecting the liver.

[0102] Embodiment 2:

[0103] S1, get 50kg fresh abalone raw material, shell, get meat and viscera group, after washing 1 time with normal temperature water, blanch with boiling water 5min, then wash 1 time with normal temperature water, drain, dry 45h under 45 ℃ of conditions, pulverize with superfine grinder after sieving, obtain particle diameter and be less than 120 purpose abalone powder.

[0104] S2, add 20% food-grade ethanol aqueous solution with a volume fraction of 3 times its weight to abalone powder, after mixing, warming to 50 DEG C, adding 250g of neutral protease, enzymolysis 2.5h under natural pH condition, constantly stirring during enzymolysis, enzymolysis ends, and obtains abalone enzymolysis solution.

[0105] S3, abalone enzymolysis liquid is centrifuged at 12000rpm for 30min, supernatant is taken, filtrate is obtained after coarse filtration with 200 mesh filter cloth, and then filtrate is ultrafiltered by ultrafiltration membrane separation equipment with molecular weight cut-off being 3000Da to obtain trapped liquid.

[0106] S4. The retentate is passed into a vacuum concentration tank and concentrated under reduced pressure at 60°C until the solid content is 20°Bx.

[0107] The concentrated solution is spray-dried and powdered at 180° C., and a product containing the liver-protecting and hangover-relieving abalone extract is obtained after packaging.

[0108] The activation rate of the retentate obtained in this example on alcohol dehydrogenase was 17.62%.

[0109] Embodiment 3:

[0110] S1, get 50kg fresh abalone raw material, shell, get meat and viscera group, after washing once with normal temperature water, blanch with boiling water for 5min, then wash once with normal temperature water, drain, dry under 55 ℃ of conditions for 40h, pulverize with superfine grinder after sieving, obtain particle diameter and be less than 120 mesh abalone powder.

[0111] S2, add 30% food-grade ethanol aqueous solution with a volume fraction of 4 times its weight to abalone powder, after mixing, warm up to 53 ° C, add 300g of neutral protease, enzymolysis 1.5h under natural pH conditions, constantly stir during enzymolysis, enzymolysis ends, and obtains abalone enzymolysis solution.

[0112] S3, abalone enzymolysis liquid is centrifuged at 12000rpm for 30min, supernatant is taken, filtrate is obtained after coarse filtration with 200 mesh filter cloth, and then filtrate is ultrafiltered by ultrafiltration membrane separation equipment with molecular weight cut-off being 3000Da to obtain trapped liquid.

[0113] S4. The retentate is passed into a vacuum concentration tank and concentrated under reduced pressure at 60°C until the solid content is 18°Bx.

[0114] The concentrated solution is spray-dried and powdered at 180° C., and a product containing the liver-protecting and hangover-relieving abalone extract is obtained after packaging.

[0115] The activation rate of the retentate obtained in this example on alcohol dehydrogenase was 18.74%.

[0116] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention without departing from the principles and purpose of the present invention.

Claims

1. A method for preparing abalone extract for protecting liver and relieving alcohol, characterized in that: include: S1, abalone powder and low concentration of ethanol are mixed to obtain mixture, and the mixture is hydrolyzed to obtain abalone hydrolyzate; S2, separating and purifying the abalone hydrolyzate obtained in S1 to obtain a retentate; S3, concentrating the retentate obtained in S2 to obtain a concentrated solution.

2. The method for preparing the liver-protecting and hangover-relieving abalone extract according to claim 1, characterized in that: The concentrated solution obtained in step S3 is further spray-dried to obtain abalone peptide powder.

3. The method for preparing the liver-protecting and hangover-relieving abalone extract according to claim 1 or 2, characterized in that: The enzymatic hydrolysis in step S1 is performed by a neutral protease that is active in low-concentration ethanol; preferably, the ethanol in step S1 is food-grade ethanol with a volume percentage of 20-30%.

4. The method for preparing the liver-protecting and hangover-relieving abalone extract according to claim 1 or 2, characterized in that: In the step S2, the mass ratio of abalone powder: low concentration ethanol: neutral protease is 1:3-5:0.005-0.

01.

5. The method for preparing the liver-protecting and hangover-relieving abalone extract according to claim 1 or 2, characterized in that: The abalone powder in step S1 is obtained by pre-treating abalone, drying and crushing the edible part of the abalone; Preferably, the pretreatment is to remove the shell of the live abalone, take out the meat and viscera, and further wash, blanch, cool and drain to obtain the edible part; Preferably, the drying temperature is 45-55°C; Preferably, the edible part of the abalone is dried until it can be crushed into powder; more preferably, the drying time is 40-48 hours.

6. The method for preparing the liver-protecting and hangover-relieving abalone extract according to claim 1 or 2, characterized in that: The temperature of enzymolysis in step S1 is 50-55° C.; preferably, the time of enzymolysis in step S1 is 1.5 h-2.5 h.

7. The method for preparing the liver-protecting and hangover-relieving abalone extract according to claim 1 or 2, characterized in that: In the step S1, the abalone hydrolysate is centrifuged to obtain a supernatant, the supernatant is coarsely filtered to obtain a filtrate, and the filtrate is ultrafiltered to obtain a retentate containing the active ingredient; Preferably, the centrifugation is performed at 10000-12000 rpm for 25-30 min; Preferably, the coarse filtration is performed using a filter cloth with a mesh size of 150-250; Preferably, the ultrafiltration is to filter the filtrate using an ultrafiltration membrane separation device with a molecular weight cutoff of 3000Da.

8. The method for preparing the liver-protecting and hangover-relieving abalone extract according to claim 1 or 2, characterized in that: The concentration of the concentrated solution is 15-20° Bx; preferably, the retentate in step S3 is concentrated at 50-60° C. to obtain the concentrated solution.

9. The abalone extract prepared according to the preparation method according to any one of claims 1 to 8 has the purpose of protecting the liver and sobering up.

10. A product containing abalone extract for protecting liver and relieving alcohol, characterized in that: The abalone extract obtained by the preparation method according to any one of claims 1 to 8 is prepared into one or more of capsules, tablets, granules, oral liquids or beverages.

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