Device and method for full-automatic culture of mycobacterium tuberculosis

Abstract

The invention discloses a device and method for full-automatic culture of mycobacterium tuberculosis in the technical field of bacterial cultivation, and the device comprises a box body, a controller is fixedly connected in the box body, a placement rack is arranged in the box body, an injection assembly is arranged on the inner top wall of the box body, and an oscillation assembly is arranged on the side, away from the injection assembly, of the inner bottom wall of the box body; a driving assembly is arranged on one side in the box body, a test tube is detachably connected into the placement frame, a first conducting strip is fixedly connected to one side of the top of the test tube, an electromagnetic valve is fixedly connected into the test tube, the injection assembly comprises a solution storage box, an electric telescopic rod is fixedly connected to the top of the solution storage box, and the other end of the electric telescopic rod is fixedly connected to the inner top wall of the box body; the bottom of the solution storage box is communicated with an electrically-driven injection needle, the other end of the spring is fixedly connected with a second conducting strip, and the second conducting strip is electrically connected with a power source. Through the driving rod, the time-delay relay, the electrically-driven injection needle and the vortex oscillator, automation from sputum sample pretreatment to mycobacterium tuberculosis culture is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of bacteria cultivation, in particular to a device and method for fully automatic cultivation of Mycobacterium tuberculosis. Background Art

[0002] Tuberculosis, as an ancient infectious disease, remains one of the major challenges in the field of global public health. As the pathogen of tuberculosis, the rapid and accurate detection and cultivation of Mycobacterium tuberculosis is of vital importance for the diagnosis, treatment monitoring and epidemic control of the disease. Traditional methods for cultivating Mycobacterium tuberculosis are often time-consuming, cumbersome, and susceptible to contamination, making it difficult to meet the needs of rapid clinical diagnosis. Therefore, the development of an efficient and automated Mycobacterium tuberculosis culture device and method has become a hot topic in current research.

[0003] In the detection process of Mycobacterium tuberculosis, sputum specimens are one of the most commonly used clinical samples, and their pretreatment steps are particularly critical. Since sputum specimens often contain a large amount of impurities and non-specific microorganisms, direct culture is prone to contamination, affecting the accuracy of the results. Therefore, the sputum specimens need to be effectively disinfected in the pretreatment stage to kill or remove most non-tuberculous mycobacteria and other microorganisms, providing a clean sample environment for subsequent culture.

[0004] At present, although there are many Mycobacterium tuberculosis culture devices on the market, most of them are still in the semi-automatic or manual operation stage, and the pre-treatment process is cumbersome and difficult to ensure consistency. In addition, traditional culture methods usually take a long time, ranging from several days to several weeks, and it is difficult to meet the needs of rapid clinical diagnosis. Therefore, it is particularly urgent to develop a Mycobacterium tuberculosis culture device that integrates sputum specimen pre-treatment, automated culture and real-time monitoring. Summary of the invention

[0005] In order to solve the above problems, the purpose of the present invention is to provide a device and method for fully automatic culture of Mycobacterium tuberculosis, which realizes automation from sputum specimen pretreatment to Mycobacterium tuberculosis culture through a driving rod, a delay relay, an electric-driven injection needle and a vortex oscillator.

[0006] In order to achieve the above-mentioned purpose, the technical scheme of the present invention is as follows: A device for fully automatic cultivation of Mycobacterium tuberculosis comprises a box body and a culture bin, an operation port for connecting the box body with the outside is opened on one side of the box body, an operation door is hinged on the outside of the operation port, a controller is fixedly connected to the box body, a placement rack is provided in the box body, the placement rack is slidably matched with the bottom wall of the box body, an injection assembly for injecting NaOH solution is provided on the top wall of the box body, an oscillation assembly for oscillating the placement rack is provided on the side of the bottom wall of the box body away from the injection assembly, a driving assembly for driving the placement rack to move is provided on one side of the box body, the driving assembly is connected to the controller signal, a test tube is detachably connected to the placement rack, a first conductive sheet is fixedly connected to one side of the top of the test tube, an electromagnetic valve is fixedly connected to the test tube, the injection assembly comprises a solution storage box, an electric telescopic rod is fixedly connected to the top of the solution storage box, the other end of the electric telescopic rod is fixedly connected to the top wall of the box body, an electric drive injection needle is connected to the bottom of the solution storage box, the electric drive injection needle is connected to the controller signal, a spring is also fixedly connected to the bottom of the solution storage box, the other end of the spring is fixedly connected to the second conductive sheet, and the second conductive sheet is electrically connected to a power supply.

[0007] The principle of the basic scheme is: NaOH solution is stored in a solution storage box, and the solution storage box is driven downward by the electric telescopic rod in the injection assembly, so that the electric-driven injection needle can be inserted into the test tube, and the solenoid valve can be energized and opened through the first conductive sheet and the second conductive sheet.

[0008] The beneficial effects of the basic scheme are: 1. Automated operation significantly reduces manual intervention and improves the efficiency and accuracy of Mycobacterium tuberculosis culture.

[0009] 2. The closed box design and automated operation process reduce the contact between samples and the external environment, reducing the risk of cross contamination.

[0010] 3. Automated devices reduce dependence on professionals and reduce labor costs

[0011] Furthermore, the driving assembly includes a driving rod, which is connected to the controller signal and slidingly cooperates with the inner wall of the box body. The other end of the driving rod is fixedly connected to the first electromagnetic sheet, and the side of the placement frame opposite to the first electromagnetic sheet is fixedly connected to the first iron sheet.

[0012] The beneficial effect of the basic scheme is: through the principle of attraction between opposite poles after the first electromagnetic sheet and the first iron sheet are energized, the driving rod can be adsorbed to the placement rack, which is conducive to the subsequent control of the driving rod to drive the movement path of the placement rack through the controller.

[0013] Furthermore, the oscillation component includes a vortex oscillator, a first connecting rod is fixedly connected to the upper end of the vortex oscillator, a second electromagnetic sheet is fixedly connected to the other end of the first connecting rod, a second iron sheet is fixedly connected to the side of the placement rack close to the oscillation component, a second connecting rod is fixedly connected to the bottom end of the vortex oscillator, and the second electromagnetic sheet corresponds to the second iron sheet.

[0014] The beneficial effect of the basic solution is that the second electromagnetic sheet and the second iron sheet attract each other, so that the position of the placement rack is fixed, and after the vortex oscillator is started, the vibration of the placement rack can be driven by the vibration of the second connecting rod.

[0015] Furthermore, a delay relay is fixedly connected to the top of the test tube, the solenoid valve is electrically connected to the delay relay, the outer cover of the electric-driven injection needle is provided with a conical light-shielding sleeve, and a UV sterilizer is fixedly connected inside the conical light-shielding sleeve. The UV sterilizer is used to disinfect the inside of the conical light-shielding sleeve, and the delay relay is used to delay closing the UV sterilizer after power is turned on, and the delay relay is used to delay opening the solenoid valve after power is turned on.

[0016] The basic scheme has the following beneficial effects: the conical light shielding sleeve is conducive to constructing a closed environment when injecting the NaOH solution, and the ultraviolet disinfector can disinfect the closed environment before the injection. After the disinfection is completed, the delay relay is conducive to controlling the time for the ultraviolet disinfector to emit ultraviolet light and the opening time of the solenoid valve. The disinfection is completed before the solenoid valve is opened, reducing the probability of the ultraviolet disinfector's ultraviolet rays killing mycobacteria in the sputum specimen.

[0017] Furthermore, a culture rack for culturing Mycobacterium tuberculosis is fixedly connected to the bottom wall of the culture chamber, and a plurality of culture dishes are placed in the culture rack.

[0018] The beneficial effect of the basic scheme is to provide a suitable growth environment and various nutrients for Mycobacterium tuberculosis through the culture dish, thereby improving the survival rate of Mycobacterium tuberculosis.

[0019] Furthermore, a temperature sensor and a heater are fixedly connected to the inner wall of the culture chamber, and the temperature sensor is connected to the controller signal.

[0020] The beneficial effects of the basic solution are: the temperature sensor is helpful for real-time monitoring of the temperature in the culture chamber, and the heater is helpful for maintaining the temperature in the culture chamber stable, thereby providing a suitable temperature for Mycobacterium tuberculosis.

[0021] Furthermore, a camera is fixedly connected to the top wall of the culture chamber.

[0022] The beneficial effect of the basic plan is that through the camera, medical staff can observe the growth of Mycobacterium tuberculosis in real time, which is conducive to the medical staff's control over the growth process of Mycobacterium tuberculosis.

[0023] Furthermore, a humidity sensor is fixedly connected to the inner wall of the culture chamber, and the humidity sensor is connected to the controller signal.

[0024] The beneficial effect of the basic solution is that the humidity sensor helps medical staff monitor whether the humidity in the culture chamber is suitable for the growth of Mycobacterium tuberculosis, thereby facilitating the regulation of the humidity in the culture chamber.

[0025] Furthermore, a gas sensor for monitoring the gas composition in the culture chamber is fixedly connected to the inner side wall of the culture chamber.

[0026] The beneficial effects of the basic scheme are: the gas sensor is conducive to real-time monitoring of the content of oxygen, carbon dioxide and other gases in the culture chamber, and the inoculation survival rate of Mycobacterium tuberculosis is improved by controlling the oxygen, carbon dioxide and other gases in the culture chamber.

[0027] A method for fully automatic cultivation of Mycobacterium tuberculosis comprises: step 1: opening an operating door and transferring a sputum specimen into a test tube on a placement rack;

[0028] Step 2: Control the driving rod through the controller to adjust the position of the test tube on the placement rack so that the solenoid valve is aligned with the electric drive injection needle;

[0029] Step three, controlling the electric telescopic rod to extend through the controller, so that the second conductive sheet is against the first conductive sheet, and the ultraviolet sterilizer and the delay relay are energized;

[0030] Step 4, controlling the closing time of the UV sterilizer and the opening time of the solenoid valve through a time delay relay;

[0031] Step 5: After the solenoid valve is turned on, the controller injects NaOH solution into the test tube through the electrically driven injection needle, and then leaves it to stand for 15 minutes;

[0032] Step 6, driving the placement rack to move to the vortex oscillator through the driving rod, so that the second electromagnetic sheet and the second iron sheet are against each other;

[0033] Step 7, the controller controls the second electromagnetic sheet to absorb the second iron sheet and then starts the vortex oscillator, and transmits vibration to the placement rack through the second connecting rod to oscillate;

[0034] Step 8: After the oscillation is completed, the driving rod moves the placement rack to the door of the box, and the medical staff can open the door to take out the sputum sample that has been pre-treated;

[0035] Step nine, sampling the sputum sample and inoculating it into a culture dish in the culture chamber for culture. During the culture process, the temperature, humidity and gas content in the culture chamber are monitored by a temperature sensor, a humidity sensor and a gas sensor to maintain the temperature at about 37°C, the oxygen content at 40%-50%, and the carbon dioxide content at 5%-10%;

[0036] Step 10: Observe the growth of the bacterial colony through a camera.

[0037] The beneficial effects of the basic scheme are: through a fully automated process, automated operations are achieved from the transfer and pretreatment of sputum specimens to the monitoring of the culture process, which significantly improves the experimental efficiency and the accuracy of operations; through real-time monitoring and adjustment of equipment such as temperature sensors, humidity sensors and gas sensors, key parameters such as temperature, humidity and gas content in the culture chamber can be accurately controlled to provide the best environment for the growth of Mycobacterium tuberculosis; the introduction of cameras realizes real-time monitoring and recording of bacterial growth, making it convenient for experimenters to observe and analyze experimental results at any time. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 1 is a diagram of the method steps for the fully automatic culture method of Mycobacterium tuberculosis in an embodiment of the present invention.

[0039] Figure 2 It is an axonometric diagram of a fully automatic culture device for Mycobacterium tuberculosis in an embodiment of the present invention.

[0040] Figure 3 It is a cross-sectional view of the casing of the fully automatic culture device for Mycobacterium tuberculosis in an embodiment of the present invention.

[0041] Figure 4 It is a cross-sectional view of a culture chamber of a fully automatic culture device for Mycobacterium tuberculosis in an embodiment of the present invention.

[0042] The figure marks in the drawings of the specification include: 1. box body; 2. conical light-shielding sleeve; 3. spring; 4. test tube; 5. driving rod; 6. placement rack; 7. culture rack; 8. culture dish; 9. culture chamber; 10. solution storage box; 11. second conductive sheet; 12. first conductive sheet; 13. vortex oscillator; 14. second connecting rod; 15. first connecting rod; 16. second electromagnetic sheet; 17. camera; 18. humidity sensor; 19. heater; 20. temperature sensor; 21. gas sensor; 22. second iron sheet. DETAILED DESCRIPTION

[0043] The following is further described in detail through specific implementation methods:

[0044] Embodiment 1:

[0045] Basically as attached Figure 2 -Attached Figure 4As shown: a device for fully automatic cultivation of Mycobacterium tuberculosis, comprising a box body 1 and a culture chamber 9, an operation port for connecting the box body 1 with the outside is opened on one side of the box body 1, an operation door is hinged on the outside of the operation port, a controller is fixedly connected to the box body 1 by bolts, a placement rack 6 is provided in the box body 1, the placement rack 6 is slidably matched with the inner bottom wall of the box body 1, an injection assembly for injecting NaOH solution is provided on the inner top wall of the box body 1, an oscillation assembly for oscillating the placement rack 6 is provided on the side of the inner bottom wall of the box body 1 away from the injection assembly, the oscillation assembly comprises a vortex oscillator 13, the upper end of the vortex oscillator 13 is fixedly connected to a first connecting rod 15 by bolts, the other end of the first connecting rod 15 is fixedly connected to a second electromagnetic sheet 16 by bolts, a second iron sheet 22 is fixedly connected to the side of the placement rack 6 close to the oscillation assembly by bolts, the bottom end of the vortex oscillator 13 is fixedly connected to a second connecting rod 14 by bolts, and the second iron sheet 22 corresponds to the second electromagnetic sheet 16.

[0046] A driving component for driving the placement rack 6 to move is provided on one side of the box body 1. The driving component is connected to the controller signal. The driving component includes a driving rod 5. The driving rod 5 is connected to the controller signal. The driving rod 5 slides with the inner wall of the box body 1. The other end of the driving rod 5 is fixedly connected to the first electromagnetic sheet by bolts, and the side of the placement rack 6 opposite to the first electromagnetic sheet is fixedly connected to the first iron sheet by bolts.

[0047] A test tube 4 is detachably connected in the placement rack 6, a first conductive sheet 12 is fixedly connected to one side of the top of the test tube 4 by bolts, a solenoid valve is fixedly connected in the test tube 4 by bolts, an injection assembly includes a solution storage box 10, an electric telescopic rod is fixedly connected to the top of the solution storage box 10 by bolts, the other end of the electric telescopic rod is fixedly connected to the top wall of the box body 1 by bolts, an electric drive injection needle is connected to the bottom of the solution storage box 10, the electric drive injection needle is connected to the controller signal, a delay relay is also fixedly connected to the top of the test tube 4 by bolts, the solenoid valve is electrically connected to the delay relay, a conical shading sleeve 2 is provided on the outer sleeve of the electric drive injection needle, an ultraviolet disinfector is fixedly connected to the top of the test tube 4 by bolts, the ultraviolet disinfector is used to disinfect the inside of the conical shading sleeve 2, the delay relay is used to delay closing the ultraviolet disinfector after power-on, the delay relay is used to delay opening the solenoid valve after power-on, a spring 3 is also fixedly connected to the bottom of the solution storage box 10 by bolts, the other end of the spring 3 is fixedly connected to the second conductive sheet 11 by bolts, and the second conductive sheet 11 is electrically connected to a power supply.

[0048] The specific implementation process is as follows: first, the medical staff needs to prepare a sputum specimen for culturing Mycobacterium tuberculosis, and then the medical staff can open the operating door, place the sputum specimen in the test tube 4 of the placement rack 6 through the operating port, and then close the operating door.

[0049] After the first electromagnetic sheet is energized, the first electromagnetic sheet will attract the first iron sheet to fix the position of the placement rack 6. The controller will first control the driving rod 5 to drive the placement rack 6 to move so as to adjust the position of the placement rack 6. When the position of the placement rack 6 is adjusted to correspond to the electromagnetic valve and the electric-driven injection needle, the controller can control the electric telescopic rod to extend so that the solution storage tank 10 moves downward. During the downward movement of the solution storage tank 10, the second conductive sheet 11 contacts the first conductive sheet 12. After the second conductive sheet 11 contacts the first conductive sheet 12, the delay relay will be energized and turned on to control the ultraviolet disinfector in the conical light-shielding sleeve 2 to emit ultraviolet rays to disinfect the electric-driven injection needle. The conical light-shielding sleeve 2 is helpful to reduce the refraction of ultraviolet light and reduce the impact of ultraviolet light on the sputum sample in the test tube. After a certain period of disinfection, the delay relay will control the ultraviolet disinfector to cut off the power, and then open the solenoid valve, so that the electric-driven injection needle can pass through the solenoid valve into the test tube 4. The solution storage box 10 stores NaOH solution. After the electric-driven injection needle enters the test tube 4, the controller can control the electric-driven injection needle to inject NaOH solution into the test tube 4. After the injection of the electric-driven injection needle is completed, the controller can control the electric telescopic rod to retract, so that the electric-driven injection needle returns to its original position. After the second conductive sheet 11 and the first conductive sheet 12 are disconnected, the solenoid valve will also be closed accordingly.

[0050] After standing for 15 minutes, the controller will drive the driving rod 5 to move along the inner wall of the box body 1. Since the first electromagnetic sheet and the first iron sheet are attracted to each other, the driving rod 5 will drive the placement rack 6 to move when it moves. When the driving rod 5 drives the placement rack 6 to move to the side close to the vortex oscillator 13, the second iron sheet 22 on the first connecting rod 15 will resist the second electromagnetic sheet 16. After power is turned on, the second iron sheet 22 and the second electromagnetic sheet 16 will be attracted to fix the placement rack 6. At this time, the second connecting rod 14 resists the placement rack 6. The continuous oscillation of the second connecting rod 14 will cause the test tube 4 in the placement rack 6 to oscillate accordingly, so that the sputum sample is fully liquefied.

[0051] After liquefaction is completed, the controller will control the driving rod 5 to move the placement rack 6 to the operation port. The medical staff can open the operation door, take out the liquefied sputum sample, and then inoculate the sputum sample on the culture dish 8 and place it in the culture chamber 9.

[0052] Embodiment 2:

[0053] The difference from the above embodiment is that the bottom wall of the culture bin 9 is fixedly connected with a culture rack 7 for culturing Mycobacterium tuberculosis by bolts, and a number of culture dishes 8 are placed in the culture rack 7. The inner wall of the culture bin 9 is fixedly connected with a temperature sensor 20 and a heater 19 by bolts, and the temperature sensor 20 is connected to the controller signal. The top wall of the culture bin 9 is fixedly connected with a camera 17 by bolts. The inner wall of the culture bin 9 is fixedly connected with a humidity sensor 18 by bolts, and the humidity sensor 18 is connected to the controller signal. The inner wall of the culture bin 9 is also fixedly connected with a gas sensor 21 for monitoring the gas composition in the culture bin 9 by bolts.

[0054] The specific implementation process is as follows: the temperature in the culture chamber 9 can be monitored in real time by the temperature sensor 20, and the temperature in the culture chamber 9 is maintained at about 37°C by the controller and the heater 19. The humidity sensor 18 can monitor the change of humidity in the culture chamber 9 in real time, which is helpful for medical staff to grasp the humidity change in the culture chamber 9 in real time. The gas sensor 21 can monitor the change of gas in the culture chamber 9 in real time, such as carbon dioxide and oxygen. Mycobacterium tuberculosis grows better when the oxygen content is maintained at 40%-50% and the carbon dioxide content is maintained at 5%-10%. Through the camera 17 in the culture chamber 9, medical staff can observe the growth of the flora in real time, realizing real-time monitoring and recording of the growth of the flora, which is convenient for experimenters to observe and analyze experimental results at any time.

[0055] Embodiment 3:

[0056] Basically as attached Figure 1 As shown: a method for fully automatic cultivation of Mycobacterium tuberculosis, comprising: step 1: opening the operating door, transferring the sputum specimen to the test tube 4 on the placement rack 6;

[0057] Step 2: Control the driving rod 5 through the controller to adjust the position of the placement rack 6 so that the solenoid valve is aligned with the electric drive injection needle;

[0058] Step 3, the controller is used to control the electric telescopic rod to extend, so that the second conductive sheet 11 and the first conductive sheet 12 are against each other, so that the ultraviolet sterilizer and the time delay relay are both energized;

[0059] Step 4, controlling the closing time of the UV sterilizer and the opening time of the solenoid valve through a time delay relay;

[0060] Step 5: After the solenoid valve is opened, the controller injects NaOH solution into the test tube 4 through the electrically driven injection needle, and then leaves it to stand for 15 minutes;

[0061] Step 6, driving the placement rack 6 to move to the vortex oscillator 13 through the driving rod 5, so that the second electromagnetic sheet 16 and the second iron sheet 22 are against each other;

[0062] Step 7, the controller controls the second electromagnetic sheet 16 to absorb the second iron sheet 22 and then starts the vortex oscillator 13, and transmits vibration to the placement rack 6 through the second connecting rod 14;

[0063] Step eight, after the oscillation is completed, the driving rod 5 moves the placement rack 6 to the door of the box, and the medical staff can open the door to take out the sputum sample that has been pre-treated;

[0064] Step nine, sampling the sputum sample and inoculating it into the culture dish 8 in the culture chamber 9 for culture. During the culture process, the temperature, humidity and gas content in the culture chamber 9 are monitored by the temperature sensor 20, the humidity sensor 18 and the gas sensor 21 to maintain the temperature at about 37°C, the oxygen content at 40%-50%, and the carbon dioxide content at 5%-10%;

[0065] Step ten, observing the growth of the bacterial colony through the camera 17.

[0066] The specific implementation process is as follows: first, the medical staff can open the box door to transfer the sputum sample to the test tube 4 on the placement rack 6, and then the controller controls the driving rod 5 to align the solenoid valve with the electric drive injection needle. After the solenoid valve is aligned with the electric drive injection needle, the controller can control the electric telescopic rod to extend so that the second conductive sheet 11 and the first conductive sheet 12 are against each other. At this time, the delay relay will control the closing time of the ultraviolet disinfector and the opening time of the solenoid valve, and the electric drive injection needle will be disinfected by the ultraviolet disinfector. The solenoid valve will not open until the ultraviolet disinfector has completed the disinfection of the electric drive injection needle. At this time, the controller can control the electric drive injection needle to inject NaOH solution into the test tube 4. After the injection is completed, the controller controls the electric telescopic rod to retract and the solenoid valve is closed.

[0067] After the electromagnetic valve is closed, the test tube 4 is left to stand for 15 minutes, and then the test tube 4 is moved to the vortex oscillator 13, and the test tube 4 is oscillated and liquefied by the vortex oscillator 13. After liquefaction is completed, the medical staff takes out the liquefied sputum specimen and inoculates it into the culture dish 8. The temperature in the culture chamber 9 is maintained at about 37°C, the oxygen content is maintained at 40%-50%, and the carbon dioxide content is maintained at 5%-10%, so as to better promote the growth of the flora. The camera 17 is conducive to real-time monitoring and recording of the growth of the flora, which is conducive to improving the medical staff's grasp of the flora culture process.

[0068] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0069] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A device for fully automatic cultivation of Mycobacterium tuberculosis, characterized in that: The invention comprises a box body (1) and a culture chamber (9), wherein an operation port for connecting the box body (1) with the outside is provided on one side of the box body (1), an operation door is hingedly connected to the outside of the operation port, a controller is fixedly connected inside the box body (1), a placement rack (6) is provided inside the box body (1), the placement rack (6) is slidably matched with the inner bottom wall of the box body (1), an injection assembly for injecting NaOH solution is provided on the inner top wall of the box body (1), an oscillation assembly for oscillating the placement rack (6) is provided on the inner bottom wall of the box body (1) away from the injection assembly, a driving assembly for driving the placement rack (6) to move is provided on one side of the box body (1), the driving assembly is connected to the controller signal, and the placement rack (6) is provided with a plurality of control components. A test tube (4) is detachably connected therein, a first conductive sheet (12) is fixedly connected to one side of the top of the test tube (4), a solenoid valve is fixedly connected therein, the injection assembly comprises a solution storage box (10), an electric telescopic rod is fixedly connected to the top of the solution storage box (10), the other end of the electric telescopic rod is fixedly connected to the top wall of the box body (1), the bottom of the solution storage box (10) is connected to an electric drive injection needle, the electric drive injection needle is connected to a controller signal, the bottom of the solution storage box (10) is also fixedly connected to a spring (3), the other end of the spring (3) is fixedly connected to a second conductive sheet (11), and the second conductive sheet (11) is electrically connected to a power source.

2. The device for fully automatic cultivation of Mycobacterium tuberculosis according to claim 1, characterized in that: The driving assembly comprises a driving rod (5), the driving rod (5) is connected to a controller signal, the driving rod (5) is slidably matched with an inner wall of a box body (1), the other end of the driving rod (5) is fixedly connected to a first electromagnetic sheet, and the side of a placement frame (6) opposite to the first electromagnetic sheet is fixedly connected to a first iron sheet.

3. The device for fully automatic cultivation of Mycobacterium tuberculosis according to claim 2, characterized in that: The oscillation component comprises a vortex oscillator (13), the upper end of the vortex oscillator (13) is fixedly connected to a first connecting rod (15), the other end of the first connecting rod (15) is fixedly connected to a second electromagnetic sheet (16), a second iron sheet (22) is fixedly connected to a side of the placement frame (6) close to the oscillation component, the bottom end of the vortex oscillator (13) is fixedly connected to a second connecting rod (14), and the second electromagnetic sheet (16) corresponds to the second iron sheet (22).

4. The device for fully automatic cultivation of Mycobacterium tuberculosis according to claim 3, characterized in that: A time delay relay is also fixedly connected to the top of the test tube (4), the electromagnetic valve is electrically connected to the time delay relay, the outer jacket of the electrically driven injection needle is provided with a conical light shielding sleeve (2), the top of the test tube (4) is fixedly connected to an ultraviolet sterilizer, the ultraviolet sterilizer is used to sterilize the inside of the conical light shielding sleeve (2), the time delay relay is used to delay closing the ultraviolet sterilizer after power is turned on, and the time delay relay is used to delay opening the electromagnetic valve after power is turned on.

5. The device for fully automatic cultivation of Mycobacterium tuberculosis according to claim 4, characterized in that: A culture rack (7) for culturing Mycobacterium tuberculosis is fixedly connected to the inner bottom wall of the culture bin (9), and a plurality of culture dishes (8) are placed in the culture rack (7).

6. The device for fully automatic cultivation of Mycobacterium tuberculosis according to claim 5, characterized in that: A temperature sensor (20) and a heater (19) are fixedly connected to the inner wall of the culture chamber (9), and the temperature sensor (20) is connected to the controller signal.

7. The device for fully automatic cultivation of Mycobacterium tuberculosis according to claim 6, characterized in that: A camera (17) is fixedly connected to the top wall of the culture chamber (9).

8. The device for fully automatic cultivation of Mycobacterium tuberculosis according to claim 7, characterized in that: A humidity sensor (18) is fixedly connected to the inner wall of the culture chamber (9), and the humidity sensor (18) is connected to the controller signal.

9. The device for fully automatic cultivation of Mycobacterium tuberculosis according to claim 8, characterized in that: The inner wall of the culture chamber (9) is also fixedly connected with a gas sensor (21) for monitoring the gas composition in the culture chamber (9).

10. A method for fully automatic cultivation of Mycobacterium tuberculosis, characterized in that: include: Step 1: Open the operating door and transfer the sputum sample to the test tube (4) on the placement rack (6); Step 2: Control the driving rod (5) through the controller to adjust the position of the placement rack (6) so that the solenoid valve is aligned with the electric drive injection needle; Step 3, controlling the electric telescopic rod to extend through the controller, so that the second conductive sheet (11) and the first conductive sheet (12) are against each other, so that the ultraviolet sterilizer and the time delay relay are both energized; Step 4, controlling the closing time of the UV sterilizer and the opening time of the solenoid valve through a time delay relay; Step 5: After the solenoid valve is opened, the controller injects NaOH solution into the test tube (4) through the electrically driven injection needle, and then the test tube (4) is left to stand for 15 minutes; Step six, driving the placement rack (6) to move to the vortex oscillator (13) through the driving rod (5), so that the second electromagnetic sheet (16) and the second iron sheet (22) are against each other; Step 7, the controller controls the second electromagnetic sheet (16) to attract the second iron sheet (22) and then starts the vortex oscillator (13), and transmits vibrations through the second connecting rod (14) to oscillate the placement rack (6); Step eight, after the oscillation is completed, the driving rod (5) moves the placement rack (6) to the door of the box, opens the door and takes out the sputum sample that has been pre-treated; Step nine, sampling the sputum sample and inoculating it into a culture dish (8) in a culture chamber (9) for culture. During the culture process, the temperature, humidity, gas content and other data in the culture chamber (9) are monitored by a temperature sensor (20), a humidity sensor (18) and a gas sensor (21), so that the temperature is maintained at about 37° C., the oxygen content is maintained at 40%-50%, and the carbon dioxide content is maintained at 5%-10%; Step 10: Observe the growth of the bacterial colony through the camera (17).

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