Multi-environment microorganism culture device and culture method thereof

Abstract

The invention relates to a multi-environment type microorganism culture device and a culture method thereof.The multi-environment type microorganism culture device comprises a base, a supporting column, a high-temperature culture chamber, a low-temperature culture chamber, a sliding groove, a fixing base and a push rod, the top face of the base is fixedly connected with the supporting column, the high-temperature culture chamber and the low-temperature culture chamber are arranged on the two sides of the supporting column respectively, and the fixing base is arranged in the high-temperature culture chamber; a sliding groove is formed in the top face of the base, the fixing base is movably connected with the sliding groove, a culture dish is fixedly installed on the fixing base, and a temperature adjusting pipeline is laid on the inner wall of the high-temperature culture chamber. Microorganisms are cultured by providing the high-temperature culture chamber and the low-temperature culture chamber with two different temperatures, so that the microorganisms are cultured in different environments at the same time, time difference is avoided, and microorganism comparison data are obtained more accurately.

Description

Technical Field

[0001] The invention belongs to the field of microorganism cultivation equipment, and in particular relates to a multi-environment microorganism cultivation device and a cultivation method thereof. Background Art

[0002] At present, in microbial monitoring operations, relevant microbial culture experiments need to be carried out. Sometimes, different types of microorganisms need to be cultured in liquid culture media of the same type and concentration, and the growth conditions of different microorganisms under the same culture conditions need to be compared. Then, the relevant optimal parameters can be screened or some corresponding reference data can be obtained.

[0003] Existing microbial culture devices can generally only provide a certain environment for cultivating microorganisms, and cannot provide different environments for cultivating organisms at the same time. When microorganisms need to be cultivated in different environments, at least two culture devices are required for cultivation, which is very troublesome. Summary of the invention

[0004] The present invention provides a multi-environment microorganism cultivation device and a cultivation method thereof to solve the problems existing in the prior art that different environments cannot be provided for simultaneous cultivation of organisms.

[0005] The technical solution adopted by the present invention to solve the technical problems existing in the known technology is: a multi-environment microorganism culture device and a culture method thereof, comprising a base, a pillar, a high-temperature culture chamber, a low-temperature culture chamber, a slide groove, a fixed seat and a push rod, the top surface of the base is fixedly connected to the pillar, and the high-temperature culture chamber and the low-temperature culture chamber are respectively arranged on both sides of the pillar, the high-temperature culture chamber is provided with a fixed seat, the top surface of the base is provided with a slide groove, the fixed seat is movably connected with the slide groove, a culture dish is fixedly installed on the fixed seat, the inner wall of the high-temperature culture chamber is paved with a temperature regulating pipe, the top of the high-temperature culture chamber is fixedly installed with a high-definition camera, and an alkali solution injection tube and an acid injection tube are arranged above the culture dish. Liquid injection tube, the left side of the pillar is fixedly connected to the first storage box, the first storage box is respectively provided with an alkaline liquid storage chamber, an acidic liquid storage chamber and a high-temperature liquid storage chamber, the high-temperature liquid storage chamber is equipped with an electric heating wire, and the bottom of the high-temperature liquid storage chamber is connected with the temperature regulating pipe through a hose, the alkaline liquid storage chamber and the acidic liquid storage chamber are respectively connected with the alkaline liquid injection tube and the acid liquid injection tube through a hose, the left side of the high-temperature cultivation chamber is rotatably connected with the door panel, the high-temperature cultivation chamber has the same structure as the low-temperature cultivation chamber, and the temperature regulating pipe in the low-temperature cultivation chamber is connected with the low-temperature liquid storage chamber through a hose, and the low-temperature liquid storage chamber is equipped with three semiconductor refrigeration plates.

[0006] Furthermore, the left end of the fixing seat is fixedly connected to the right end of the push rod, and the left end of the push rod is fixedly connected to a handle.

[0007] Furthermore, a display screen is fixedly mounted on the support, and the high-definition camera is electrically connected to the display screen.

[0008] Furthermore, the high-temperature liquid storage chamber, the alkaline liquid storage chamber, the acidic liquid storage chamber, the semiconductor refrigeration plate and the top of the low-temperature liquid storage chamber are all provided with liquid injection ports.

[0009] Furthermore, the hoses between the alkaline liquid injection tube and the acid liquid injection tube and the alkaline liquid storage chamber and the acid liquid storage chamber are all installed with electronic valves.

[0010] Furthermore, a clamping block is fixedly connected to one end of the fixing seat close to the pillar.

[0011] The present invention provides a multi-environment microbial culture device and a culture method thereof, comprising the following steps: Step 1: Pull the fixed seat by the handle on the push rod to pull the culture dish out of the high temperature culture chamber or the low temperature culture chamber; The second step: put the microbial strains into the culture dish, and then push the culture dish into the high-temperature culture chamber or the low-temperature culture chamber through the push rod, and then supply power to the heating wire to make the heating wire heat up to heat the water in the high-temperature culture chamber, and transfer heat through the temperature regulating pipe to increase the temperature inside the high-temperature culture chamber, or supply power to the semiconductor refrigeration chip to cool the liquid in the low-temperature liquid storage chamber, and lower the temperature in the low-temperature liquid storage chamber through the temperature regulating pipe.

[0012] Step 3: By controlling the electronic valves between the alkaline liquid injection tube and the acid liquid injection tube and the alkaline liquid storage chamber and the acid liquid storage chamber, the amount of acid and alkali liquid dripping into the culture dish is realized, thereby controlling the pH value of the culture dish.

[0013] Step 4: Use a high-definition camera to capture the growth of microbial colonies in the culture dish and observe it on the display screen.

[0014] The advantages and positive effects of the present invention are as follows: 1. This multi-environment microbial culture device cultivates microorganisms by providing two culture chambers with different temperatures, a high-temperature culture chamber and a low-temperature culture chamber, so as to cultivate microorganisms in different environments at the same time without time difference, and obtain microbial comparison data more accurately.

[0015] 2. This multi-environment microbial cultivation device captures the cultivation status of microorganisms by setting up a high-definition camera, and intuitively reflects it through a display screen, which is convenient for personnel to observe. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is an overall structural diagram of a device provided by an embodiment of the present invention; In the figure: 1. base, 2. support, 3. high temperature culture chamber, 4. low temperature culture chamber, 5. slide, 6. fixed seat, 7. push rod, 8. block, 9. culture dish, 10. door panel, 11. temperature regulating pipe, 12. high-definition camera, 13. alkali liquid injection tube, 14. acid liquid injection tube, 15. first storage box, 16. second storage box, 17. heating wire, 18. high temperature liquid storage chamber, 19. alkaline liquid storage chamber, 20. acid liquid storage chamber, 21. semiconductor refrigeration sheet, 22. low temperature liquid storage chamber, 23. display screen. DETAILED DESCRIPTION

[0017] In order to further understand the content, features and effects of the present invention, the following embodiments are listed and described in detail with reference to the accompanying drawings.

[0018] Combine the following Figure 1An integrated fresh milk microbial detector of the present invention is described in detail: an integrated fresh milk microbial detector comprises a base 1, a pillar 2, a high temperature culture chamber 3, a low temperature culture chamber 4, a chute 5, a fixed seat 6 and a push rod 7, wherein the top surface of the base 1 is fixedly connected to the pillar 2, and the high temperature culture chamber 3 and the low temperature culture chamber 4 are respectively arranged on both sides of the pillar 2, the high temperature culture chamber 3 is equipped with a fixed seat 6, the top surface of the base 1 is provided with a chute 5, the fixed seat 6 is movably connected with the chute 5, a culture dish 9 is fixedly installed on the fixed seat 6, and the inner wall of the high temperature culture chamber 3 is paved with a temperature The high temperature culture chamber 3 is provided with a temperature regulating pipe 11, a high definition camera 12 is fixedly installed on the top of the high temperature culture chamber 3, an alkaline liquid injection pipe 13 and an acid liquid injection pipe 14 are arranged above the culture dish 9, the left side of the pillar 2 is fixedly connected to the first storage box 15, and the first storage box 15 is provided with an alkaline liquid storage chamber 19, an acid liquid storage chamber 20 and a high temperature liquid storage chamber 18, the high temperature liquid storage chamber 18 is provided with an electric heating wire 17, and the bottom of the high temperature liquid storage chamber 18 is connected to the temperature regulating pipe 11 through a hose, and the alkaline liquid storage chamber 19 and the acid liquid storage chamber 20 are connected. The high temperature culture chamber 3 is connected to the alkaline liquid injection tube 13 and the acid liquid injection tube 14 through a hose respectively, and the acidic liquid and the alkaline liquid are dripped through the alkaline liquid injection tube 13 and the acid liquid injection tube 14 to adjust the pH value of the culture dish. The left side of the high temperature culture chamber 3 is rotatably connected to the door panel 10. The high temperature culture chamber 3 has the same structure as the low temperature culture chamber 4, and the temperature regulating pipe 11 in the low temperature culture chamber 4 is connected to the low temperature liquid storage chamber 22 through a hose. The low temperature liquid storage chamber 22 has three semiconductor refrigeration sheets built in. The left end of the fixing seat 6 is fixedly connected to the right end of the push rod 7, and the left end of the push rod 7 is fixedly connected to a handle. A display screen 23 is fixedly installed on the pillar 2, and the high-definition camera 12 is electrically connected to the display screen 23. The image of the colony monitored by the high-definition camera 12 is visually monitored through the display screen 23. Liquid injection ports are provided on the tops of the high-temperature liquid storage chamber 18, the alkaline liquid storage chamber 19, the acidic liquid storage chamber 20, the semiconductor refrigeration plate 21 and the low-temperature liquid storage chamber 22. Electronic valves are installed on the hoses between the alkaline liquid injection tube 13 and the acid liquid injection tube 14 and the alkaline liquid storage chamber 19 and the acidic liquid storage chamber 20. A block 8 is fixedly connected to the fixing seat 6 near one end of the pillar 2.

[0019] The present invention provides a multi-environment microbial culture device and a culture method thereof, comprising the following steps: Step 1: Pull the fixing seat 6 by the handle on the push rod 7, and then pull the culture dish 9 out of the high temperature culture chamber 3 or the low temperature culture chamber 4; Step 2: Place the microbial strains into the culture dish 9, and then push the culture dish 9 into the high-temperature culture chamber 3 or the low-temperature culture chamber 4 through the push rod 7, and then supply power to the heating wire 17 to make the heating wire 17 generate heat to heat the water in the high-temperature culture chamber 3, and transfer heat through the temperature regulating pipe 11 to increase the temperature inside the high-temperature culture chamber 3, or supply power to the semiconductor refrigeration sheet 21 to cool the liquid in the low-temperature liquid storage chamber 22, and reduce the temperature inside the low-temperature liquid storage chamber 22 through the temperature regulating pipe 11.

[0020] Step 3: By controlling the electronic valves between the alkaline liquid injection tube 13 and the acid liquid injection tube 14 and the alkaline liquid storage chamber 19 and the acid liquid storage chamber 20, the amount of acid and alkali liquid dripping into the culture dish 9 is realized, thereby controlling the pH value of the culture dish 9.

[0021] Step 4: The growth of the microbial colonies in the culture dish 9 is captured by the high-definition camera 12 and observed through the display screen 23 .

[0022] Working principle: by providing two culture chambers with different temperatures, a high-temperature culture chamber 3 and a low-temperature culture chamber 4, microorganisms are cultured, so that microorganisms can be cultured in different environments at the same time without time difference, and microbial comparison data can be obtained more accurately. A high-definition camera 12 is set to capture the culture status of microorganisms, and it is intuitively reflected through the display screen 23, which is convenient for personnel to observe.

[0023] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Any simple modification, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are within the scope of the technical solution of the present invention.

Claims

1. A multi-environment microorganism culture device, comprising a base (1), a support (2), a high-temperature culture chamber (3), a low-temperature culture chamber (4), a slide (5), a fixing seat (6) and a push rod (7), characterized in that: The top surface of the base (1) is fixedly connected to the support (2); a high-temperature culture chamber (3) and a low-temperature culture chamber (4) are respectively arranged on both sides of the support (2); a fixed seat (6) is built into the high-temperature culture chamber (3); a slide groove (5) is provided on the top surface of the base (1); the fixed seat (6) is movably connected to the slide groove (5); a culture dish (9) is fixedly installed on the fixed seat (6); a temperature regulating pipe (11) is laid on the inner wall of the high-temperature culture chamber (3); a high-definition camera (12) is fixedly installed on the top of the high-temperature culture chamber (3); an alkali liquid injection pipe (13) and an acid liquid injection pipe (14) are arranged above the culture dish (9); the left side of the support (2) is fixedly connected to a first storage box (15); and the first storage box (15) is respectively provided with a plurality of An alkaline liquid storage chamber (19), an acidic liquid storage chamber (20) and a high-temperature liquid storage chamber (18); the high-temperature liquid storage chamber (18) is provided with a heating wire (17), and the bottom of the high-temperature liquid storage chamber (18) is connected to a temperature regulating pipe (11) via a hose; the alkaline liquid storage chamber (19) and the acidic liquid storage chamber (20) are respectively connected to an alkaline liquid injection pipe (13) and an acid liquid injection pipe (14) via a hose; the left side of the high-temperature culture chamber (3) is rotatably connected to a door panel (10); the high-temperature culture chamber (3) and the low-temperature culture chamber (4) have the same structure; the temperature regulating pipe (11) in the low-temperature culture chamber (4) is connected to a low-temperature liquid storage chamber (22) via a hose; and the low-temperature liquid storage chamber (22) is provided with three semiconductor cooling plates.

2. The multi-environment microorganism cultivation device according to claim 1, characterized in that: The left end of the fixing seat (6) is fixedly connected to the right end of the push rod (7), and the left end of the push rod (7) is fixedly connected to a handle.

3. The multi-environment microorganism cultivation device according to claim 1, characterized in that: A display screen (23) is fixedly mounted on the support (2), and the high-definition camera (12) is electrically connected to the display screen (23).

4. The multi-environment microorganism cultivation device according to claim 1, characterized in that: Liquid injection ports are provided at the tops of the high-temperature liquid storage chamber (18), the alkaline liquid storage chamber (19), the acidic liquid storage chamber (20), the semiconductor refrigeration plate (21) and the low-temperature liquid storage chamber (22).

5. The multi-environment microorganism cultivation device according to claim 1, characterized in that: The hoses between the alkaline liquid injection tube (13) and the acid liquid injection tube (14) and the alkaline liquid storage chamber (19) and the acid liquid storage chamber (20) are all installed with electronic valves.

6. The multi-environment microorganism cultivation device according to claim 1, characterized in that: A clamping block (8) is fixedly connected to one end of the fixing seat (6) close to the pillar (2).

7. A multi-environment microorganism cultivation device according to any one of claims 1 to 5, characterized in that: The multi-environment microbial culture device and culture method thereof include the following steps: Step 1: Pull the fixing seat (6) by the handle on the push rod (7), thereby pulling the culture dish (9) out of the high temperature culture chamber (3) or the low temperature culture chamber (4); Step 2: placing the microorganism strain into a culture dish (9), and then pushing the culture dish (9) into a high-temperature culture chamber (3) or a low-temperature culture chamber (4) through a push rod (7), and then supplying power to the heating wire (17) to heat the water in the high-temperature culture chamber (3), and heat the water in the high-temperature culture chamber (3) through a temperature regulating pipe (11), or supplying power to a semiconductor refrigeration chip (21) to cool the liquid in the low-temperature liquid storage chamber (22), and lowering the temperature in the low-temperature liquid storage chamber (22) through the temperature regulating pipe (11); Step 3: by controlling the electronic valves between the alkaline liquid injection tube (13) and the acid liquid injection tube (14) and the alkaline liquid storage chamber (19) and the acid liquid storage chamber (20), the amount of acid and alkali liquid dripped into the culture dish (9) is achieved, thereby achieving control of the pH value of the culture dish (9); Step 4: The growth of the microbial colony in the culture dish (9) is captured by a high-definition camera (12) and observed through a display screen (23).

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