Seedling recovering and root promoting type compound microbial agent and application thereof in bio-organic fertilizer

Abstract

The invention provides a complex microbial inoculant. The complex microbial inoculant comprises active ingredients, the active ingredients comprise bacillus megaterium and bacillus velezensis, the preservation number of the bacillus megaterium is CGMCC (China General Microbiological Culture Collection Center) No.21828, and the preservation number of the bacillus velezensis is CGMCC No.28558. The complex microbial inoculant can remarkably promote growth of root systems of crops in the seedling stage and effectively solve the problem of slow seedling recovery during crop transplanting.

Description

Technical Field

[0001] The present invention relates to the field of agricultural microorganisms, and in particular to a composite microbial agent for slowing seedling growth and promoting rooting, and its application in biological organic fertilizer. Background Art

[0002] Soil is the basis of agricultural production, and soil quality has an important impact on the growth and yield of crops. However, due to unreasonable utilization, climate change and other reasons, the soil quality has declined, especially the saline-alkali soil in some areas, which has a serious impact on the growth and yield of crops. Therefore, improving the quality of saline-alkali soil has become an urgent problem to be solved in current agricultural production. At present, chemical fertilizers and organic fertilizers are mainly used to improve saline-alkali soil. Although chemical fertilizers can quickly improve soil quality, long-term use will lead to problems such as soil compaction and acidification, and cause certain pollution to the environment. Although organic fertilizers can improve soil structure and increase soil fertility, they have poor improvement effects on saline-alkali soil. Therefore, for the improvement of saline-alkali soil, it is necessary to find a method that can improve saline-alkali soil without polluting the environment.

[0003] The biological organic fertilizer prepared by the composite microbial agent contains rich organic matter and microbial strains, which can improve the physical and chemical properties of the soil, increase the organic matter content of the soil, improve the disease resistance of crops, and improve the fertility of the soil. The organic matter and microbial strains can promote the decomposition and reproduction of microorganisms in the soil, increase the activity of the soil, and thus promote the growth of crops. It can also decompose the organic matter in the soil, release the nutrients in the soil, thereby increasing the nutrient content in the soil and improving the yield and quality of crops. Some microbial strains can produce acidic substances and lower the pH value of the soil, thereby improving the acid-base balance of saline-alkali land and making the soil more suitable for crop growth. There are also some microbial strains that can produce salt-alkali resistant enzymes and hormones to improve the salt-alkali tolerance of crops, thereby increasing the adaptability of crops.

[0004] In short, the biological organic fertilizer prepared from composite microbial agents plays a very important role in the improvement of saline-alkali land. It can not only improve the quality of the soil, increase the fertility and activity of the soil, but also reduce the pH value of the soil, improve the disease resistance of crops, increase the nutrients in the soil, and improve the salt-alkali tolerance of crops, thereby increasing the yield and quality of crops. Summary of the invention

[0005] The present invention aims to solve at least one of the technical problems in the related art to a certain extent. To this end, the present invention proposes a composite bacterial agent. According to an embodiment of the present invention, the composite bacterial agent includes: an active ingredient, the active ingredient includes Bacillus megaterium and Bacillus velez, the Bacillus megaterium has a preservation number of CGMCC No.21828, and the Bacillus velez has a preservation number of CGMCC No.28558. The composite bacterial agent according to the embodiment of the present invention can significantly promote the growth of the root system of crops in the seedling stage, and effectively improve the problem of slow seedling growth after crop transplanting.

[0006] In the second aspect of the present invention, the present invention proposes a compound fertilizer. According to an embodiment of the present invention, the compound fertilizer includes the aforementioned composite bacterial agent and plant-based organic fertilizer. The compound fertilizer according to the embodiment of the present invention has a significant growth-promoting effect on a variety of crops, significantly increases crop yields, and can effectively improve the physical and chemical properties of saline-alkali soils and effectively prevent and control crop diseases. The field application effect is significant and can be used in production.

[0007] In the third aspect of the present invention, the present invention proposes the use of the aforementioned composite microbial agent or the aforementioned composite fertilizer in promoting the growth of crops or preventing and controlling crop diseases. The composite microbial agent or composite fertilizer according to the embodiments of the invention has a significant growth-promoting effect on crops, especially on tomatoes, peppers, and cabbage, can significantly increase crop yields, effectively improve the physical and chemical properties of saline-alkali soils, and effectively prevent and control crop diseases, especially potato early blight, potato wilt, and potato scab.

[0008] In a fourth aspect of the present invention, the present invention provides a method for promoting the growth of crops or preventing and controlling crop diseases. According to an embodiment of the present invention, the method comprises applying the aforementioned composite bacterial agent or the aforementioned composite fertilizer to the crops. The method according to an embodiment of the present invention can significantly promote the growth of the root system of crops in the seedling stage, effectively improve the problem of slow seedling growth after crop transplanting, and can also effectively prevent and control the occurrence and development of crop diseases. DETAILED DESCRIPTION

[0009] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and intended to be used to explain the present invention, but should not be construed as limiting the present invention.

[0010] In the first aspect of the present invention, the present invention proposes a composite bacterial agent. According to an embodiment of the present invention, the composite bacterial agent includes: an active ingredient, the active ingredient includes Bacillus megaterium and Bacillus velezensis, the Bacillus megaterium is Bacillus megaterium, the deposit number is CGMCC No.21828, the deposit unit is the General Microbiology Center of the China Microorganism Culture Collection Administration, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the deposit time is February 2021. The Bacillus velezensis is Bacillus velezensis, named BLS809, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on September 26, 2023, with an address of No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and a deposit number of CGMCCNo.28558.

[0011] The composite bacterial agent according to the embodiment of the present invention can significantly promote the growth of the root system of crops in the seedling stage, and effectively improve the problem of slow seedling growth after crop transplanting.

[0012] According to an embodiment of the present invention, the composite bacterial agent may further include at least one of the following additional technical features:

[0013] According to an embodiment of the present invention, the mass ratio of the Bacillus megaterium and the Bacillus velez is (0.5-1): (0.5-1), preferably 1: 1. For example, the mass ratio of the Bacillus megaterium and the Bacillus velez is 0.5: 1, 1: 0.5 or 1: 1. Among them, Bacillus megaterium has a phosphate-solubilizing effect, and can dissolve insoluble phosphates through its metabolites (such as organic acids, protons and polysaccharides), which is beneficial to soil improvement and plant nutrition. Bacillus velez can resist plant diseases and insect pests and promote plant growth. The inventors found that when the mass ratio of the Bacillus megaterium and the Bacillus velez is within the range of (0.5-1): (0.5-1), the synergistic effect of the two is significant, and the effect is significantly better in promoting the growth of the root system of the crop seedling stage, and the control effect of plant diseases is also significantly better.

[0014] According to an embodiment of the present invention, based on the total mass of the composite microbial agent, the amount of live bacteria of the active ingredient is 60 billion / g-100 billion / g. The inventors found that when the amount of live bacteria is within the above range, the composite microbial agent is significantly more effective in promoting the growth of crop root systems at the seedling stage and is also significantly more effective in preventing and controlling plant diseases.

[0015] According to an embodiment of the present invention, the ratio of the live bacteria amount of the Bacillus megaterium to the Bacillus Velez subtilis is (2-10):(6-15), for example, the ratio of the live bacteria amount of the Bacillus megaterium to the Bacillus Velez subtilis is preferably (6-10):(6-10) or (2-3):(8-10) or (3-5):(10-15), preferably (3-5):(10-15), for example, 3:10, 3:11, 3:12, 3:13, 3:14, 3:15, 4:10, 4:11, 4:12, 4:13, 4:14, 4:15, 5:10, 5:11, 5:12, 5:13, 5:14, 5:15. Bacillus megaterium has a phosphate-solubilizing effect and can dissolve insoluble phosphates through its metabolites (such as organic acids, protons and polysaccharides), which is beneficial to soil improvement and plant nutrition. Bacillus velez mainly produces antibacterial effects by secreting lipopeptide antibiotics, polyketide compounds and antibacterial proteins, etc., to fight plant diseases and insect pests and promote plant growth. The inventors found that the ratio of the live bacteria of the Bacillus megaterium and the Bacillus velez has a significant effect on the growth and disease control effect of crops. If the ratio is too high, the phosphate-solubilizing effect will be too strong, and the effect of inhibiting plant diseases and insect pests will be insufficient, which may affect the plant's absorption balance of nutrients and the control effect of plant diseases, thereby affecting the overall health and yield of crops. If the ratio is too low, plant diseases and insect pests will be overly inhibited, but the effects of soil improvement and plant nutrition will be insufficient, which may also lead to insufficient plant nutrition and affect the growth and yield of crops. The ratio of the live bacteria of the Bacillus megaterium and the Bacillus velez is in the range of (3-5): (10-15), which can maximize the synergistic effect of the two. This balance can ensure soil improvement and plant nutrition while effectively combating plant diseases and insect pests and promoting plant growth. Studies have shown that the ratio of the live bacteria of the Bacillus megaterium to the Bacillus velezinii in the range of (3-5): (10-15) can significantly increase the root length, plant height and fresh weight of crops, increase the content of chlorophyll, soluble sugar and soluble protein, thereby promoting plant growth. In addition, this balance can also enhance the disease resistance of plants and improve crop yield and quality.

[0016] According to an embodiment of the present invention, the composite bacterial agent further includes auxiliary materials.

[0017] According to an embodiment of the present invention, the auxiliary material includes at least one of calcium carbonate or diatomaceous earth. For example, the calcium carbonate may be light calcium carbonate.

[0018] According to an embodiment of the present invention, the mass ratio of the excipient to the active ingredient is (0.5-2):(0.5-2), for example, 0.5:1, 0.5:2, 1:0.5, 1:2, 2:0.5, 2:1 or 1:1, preferably 1:1.

[0019] According to an embodiment of the present invention, the preparation method of the composite bacterial agent is as follows:

[0020] 1) Seed solution preparation: Bacillus megaterium and Bacillus velez preserved in the plate were inoculated into a 250 mL Erlenmeyer flask containing 100 mL LB culture solution using an inoculation loop, respectively, and cultured in a constant temperature incubator at 37° C., 150-250 rpm for 6 h until the logarithmic growth phase, when the OD600 value was 0.5-0.8, and the seed solution concentration was not less than 100 million / mL, thereby obtaining Bacillus megaterium seed solution and Bacillus velez seed solution inoculated into the fermentation medium, respectively.

[0021] 2) Fermentation medium (the following are all in mass percentage):

[0022] Formula A: 2% soybean meal, 3% corn flour, 0.5% starch, 0.25% glucose, 0.10% sodium chloride, 0.2% dipotassium hydrogen phosphate, 0.01% manganese sulfate, 0.3% defoaming agent, and the balance is water. The pH is adjusted to 7.0. Under 0.1MPa conditions, sterilize at 121℃ together with the fermentation tank for 60min, and then cool to normal pressure and temperature under reduced pressure for use.

[0023] Formula B: 2.5%-3% corn starch, 0.5%-1% sucrose, 3%-4% soybean meal, 0.10%-0.20% yeast powder, 0.15%-0.20% peptone, 0.3%-0.4% dipotassium hydrogen phosphate, 0.2%-0.3% potassium dihydrogen phosphate, 0.1%-0.2% calcium carbonate, 0.1%-0.15% sodium chloride, 0.2%-0.3% defoaming agent, the balance is water, the pH is adjusted to 7.0, under 0.1MPa conditions, sterilize at 121℃ together with the fermentation tank for 60min, and cool to normal pressure and temperature under reduced pressure for use.

[0024] 3) Liquid fermentation: The Bacillus megaterium seed solution and the Bacillus velezium seed solution prepared in step 1) are inoculated into pilot fermentation tanks (10L-50L-500L) respectively, wherein the Bacillus megaterium adopts fermentation medium A and the Bacillus velezium adopts fermentation medium B, and the initial concentration of the bacteria in each fermentation culture system is ensured to be not less than 1 million / mL, and the tank pressure is maintained at 0.05-0.10KPa at 35-37°C, the dissolved oxygen content is ensured to be 20%-25%, the rotation speed is 180r / min, the pH value is 7.0-8.0, and the culture is carried out for 36h-48h. After the fermentation, Bacillus megaterium and Bacillus velezium are obtained respectively, the spore production rate is more than 90%, and the number of viable bacteria in the fermentation liquid is not less than 5 billion / ml and 15 billion / ml respectively.

[0025] 4) Adding 2%-5% light calcium carbonate to the fermentation liquid prepared in step 3) in proportion, drying and packaging through a spray drying device, so as to prepare a composite bacterial agent of Bacillus megaterium and Bacillus velez that can be added to fertilizers, wherein the number of viable bacteria in the bacterial agent preparations reaches 30 billion / g-50 billion / g for Bacillus megaterium and 100 billion / g-150 billion / g for Bacillus velez.

[0026] 5) The bacterial preparation prepared in step 4) is fully mixed with Bacillus megaterium powder and Bacillus velez powder in a ratio of 1:1 to obtain a composite microbial bacterial preparation with a viable cell count of 60 billion / g to 100 billion / g.

[0027] In the second aspect of the present invention, the present invention proposes a compound fertilizer. According to an embodiment of the present invention, the compound fertilizer includes the aforementioned composite bacterial agent and plant-based organic fertilizer. The compound fertilizer according to the embodiment of the present invention has a significant growth-promoting effect on a variety of crops, significantly increases crop yields, and can effectively improve the physical and chemical properties of saline-alkali soils and effectively prevent and control crop diseases. The field application effect is significant and can be used in production.

[0028] According to an embodiment of the present invention, the compound fertilizer may further include at least one of the following additional technical features:

[0029] According to an embodiment of the present invention, the plant-based organic fertilizer is prepared by the following method: solid composting mushroom residues, decomposing and fermenting, drying, crushing, and screening to prepare plant-based organic fertilizer, wherein the organic matter content is ≥40%, the water content is ≤30%, and the pH is 5.5-8.5.

[0030] According to an embodiment of the present invention, the mass ratio of the composite microbial agent and the plant-derived organic fertilizer is (3-5): 10000. The inventors found that the mass ratio of the composite microbial agent and the plant-derived organic fertilizer has a significant effect on the control effect of the composite fertilizer on plant diseases. When the mass ratio of the composite microbial agent and the plant-derived organic fertilizer is too large, the nutrients in the organic fertilizer will be over-consumed, affecting the absorption of nutrients by plants. At the same time, too many microorganisms may compete with plant roots for nutrients and living space, thereby inhibiting plant growth. In addition, an excessive amount of composite microbial agent may cause an imbalance of soil microorganisms, affecting soil health and function. When the mass ratio of the composite microbial agent and the plant-derived organic fertilizer is too small, the biological activity of the composite microbial agent cannot be fully exerted, such as promoting plant growth, improving soil fertility and inhibiting pathogens, which may lead to a decrease in the decomposition and conversion efficiency of the plant-derived organic fertilizer, thereby affecting the growth and yield of crops. When the mass ratio of the composite microbial agent and the plant-derived organic fertilizer is within the range of (3-5): 10000, the synergistic effect of the two can be maximized. The composite microbial agent can effectively promote the decomposition of plant-derived organic fertilizer, increase the organic matter content in the soil, improve the soil structure and biological function, and provide the nutrients required for plant growth. At the same time, the composite microbial agent can also enhance the disease resistance of plants and improve crop yield and quality. Studies have shown that a reasonable ratio of composite microbial agents to organic fertilizers can significantly increase the root length, plant height and fresh weight of crops, increase the content of chlorophyll, soluble sugars and soluble protein, thereby promoting plant growth.

[0031] According to an embodiment of the present invention, based on the total mass of the compound fertilizer, the amount of live bacteria is not less than 20 million / g.

[0032] In the third aspect of the present invention, the present invention proposes the use of the aforementioned composite microbial agent or the aforementioned composite fertilizer in promoting the growth of crops or preventing and controlling crop diseases. The composite microbial agent or composite fertilizer according to the embodiments of the invention has a significant growth-promoting effect on crops, especially on tomatoes, peppers, and cabbage, can significantly increase crop yields, effectively improve the physical and chemical properties of saline-alkali soils, and effectively prevent and control crop diseases, especially potato early blight, potato wilt, and potato scab.

[0033] According to an embodiment of the present invention, the above-mentioned use may further include at least one of the following additional technical features:

[0034] According to an embodiment of the present invention, the crop growth includes at least one of root growth and aboveground growth of the crop.

[0035] According to an embodiment of the present invention, the crop includes at least one of corn, rice, tomato, pepper, cabbage and potato.

[0036] According to an embodiment of the present invention, the crop growth includes at least one of corn, rice, tomato, pepper and cabbage, preferably tomato, pepper and cabbage.

[0037] According to an embodiment of the present invention, the crop diseases include at least one of potato early blight, potato wilt, and potato scab.

[0038] The composite microbial agent according to the embodiment of the present invention is used in the cultivation of peppers and tomatoes to promote root growth in the seedling stage and improve the problem of slow seedling growth after transplanting. The specific effect is that in a potted plant experiment, compared with no addition of microbial agents, the root fresh weight of the composite microbial agent treatment group increased by 10.1%-18.2%, and the aboveground biomass increased by 8.5%-11.7%.

[0039] The compound fertilizer according to the embodiment of the present invention has a significant growth-promoting effect on various crops such as tomatoes, peppers, cabbage, etc., and can effectively improve the physical and chemical properties of the soil. The specific effects are as follows: in the potted plant test, the compound fertilizer treatment with the addition of the compound bacterial agent increased the yield by 6.7%-9.2%, the soil organic matter content increased by 10.8%-28.3%, and the soil EC value decreased by 6.7%-10.5% compared with the compound fertilizer treatment without the addition of the compound bacterial agent; in the field test, the compound fertilizer treatment with the addition of the compound bacterial agent improved the prevention and control effect of potato early blight, potato wilt, and potato scab by 16.2%-42.2%, the yield increased by 8.8%-16.5%, and the soil organic matter content increased by 5.3%-8.1%, and the field application effect was significant.

[0040] In a fourth aspect of the present invention, the present invention provides a method for promoting the growth of crops or preventing and controlling crop diseases. According to an embodiment of the present invention, the method comprises applying the aforementioned composite bacterial agent or the aforementioned composite fertilizer to the crops. The method according to an embodiment of the present invention can significantly promote the growth of the root system of crops in the seedling stage, effectively improve the problem of slow seedling growth after crop transplanting, and can also effectively prevent and control the occurrence and development of crop diseases.

[0041] According to an embodiment of the present invention, the above method may further include at least one of the following additional technical features:

[0042] According to an embodiment of the present invention, the compound bacterial agent or the compound fertilizer is applied to at least one of the root and the leaf of the crop.

[0043] According to an embodiment of the present invention, the application amount of the composite bacterial agent is 100-200 billion CFU / mu. Converted to mass, the application amount of the composite bacterial agent is 2-4 kg / mu.

[0044] According to an embodiment of the present invention, the application amount of the compound fertilizer is 30-60 kg / mu.

[0045] The present invention will be further described in detail below in conjunction with specific embodiments. The specific embodiments described below are exemplary and intended to be used to explain the present invention, but should not be construed as limiting the present invention.

[0046] Example 1 Preparation of composite microbial agent

[0047] A method for preparing a composite microbial agent is as follows:

[0048] 1) Seed solution preparation: Bacillus megaterium and Bacillus velez preserved in the plate were inoculated into a 250 mL flask containing 100 mL LB culture medium using an inoculation loop, respectively, and cultured in a constant temperature incubator at 37°C, 150-250 rpm for 6 h until the logarithmic growth phase. The OD 600 The value is 0.5-0.8, the seed solution concentration is not less than 100 million / mL, and the Bacillus megaterium seed solution and the Bacillus velez seed solution inoculated into the fermentation medium are obtained respectively.

[0049] 2) Fermentation medium (the following are all in mass percentage):

[0050] Formula A: 2% soybean meal, 3% corn flour, 0.5% starch, 0.25% glucose, 0.10% sodium chloride, 0.2% dipotassium hydrogen phosphate, 0.01% manganese sulfate, 0.3% defoaming agent, and the balance is water. The pH is adjusted to 7.0. Under 0.1MPa conditions, sterilize at 121℃ together with the fermentation tank for 60min, and then cool to normal pressure and temperature under reduced pressure for use.

[0051] Formula B: 2.5%-3% corn starch, 0.5%-1% sucrose, 3%-4% soybean meal, 0.10%-0.20% yeast powder, 0.15%-0.20% peptone, 0.3%-0.4% dipotassium hydrogen phosphate, 0.2%-0.3% potassium dihydrogen phosphate, 0.1%-0.2% calcium carbonate, 0.1%-0.15% sodium chloride, 0.2%-0.3% defoaming agent, the balance is water, the pH is adjusted to 7.0, under 0.1MPa conditions, sterilize at 121℃ together with the fermentation tank for 60min, and cool to normal pressure and temperature under reduced pressure for use.

[0052] 3) Liquid fermentation: The Bacillus megaterium seed solution and the Bacillus velezium seed solution prepared in step 1) are inoculated into pilot fermentation tanks (10L-50L-500L) respectively, wherein the Bacillus megaterium adopts fermentation medium A and the Bacillus velezium adopts fermentation medium B, and the initial concentration of the bacteria in each fermentation culture system is ensured to be not less than 1 million / ml, and the tank pressure is maintained at 0.05-0.10KPa at 35-37°C, the dissolved oxygen content is ensured to be 20%-25%, the rotation speed is 180r / min, the pH value is 7.0-8.0, and the culture is carried out for 36h-48h. After the fermentation, Bacillus megaterium and Bacillus velezium are obtained respectively, the spore production rate is more than 90%, and the number of viable bacteria in the fermentation liquid is not less than 5 billion / ml and 15 billion / ml respectively.

[0053] 4) Adding 2%-5% light calcium carbonate to the fermentation liquid prepared in step 3) in proportion, drying and packaging through a spray drying device, so as to prepare a composite bacterial agent of Bacillus megaterium and Bacillus velez for use in fertilizers, wherein the number of viable bacteria in the bacterial agent preparations reaches 30 billion / g-50 billion / g for Bacillus megaterium and 100 billion / g-150 billion / g for Bacillus velez.

[0054] 5) The bacterial preparation prepared in step 4) is fully mixed with Bacillus megaterium powder and Bacillus velez powder in a ratio of 1:1 to obtain a composite microbial bacterial preparation with a viable cell count of 60 billion / g to 100 billion / g.

[0055] Example 2 Preparation of compound fertilizer

[0056] The preparation method of compound fertilizer is as follows:

[0057] 1) The mushroom residue is subjected to solid composting, decomposition and fermentation, drying, crushing and screening to prepare a plant-based organic fertilizer, wherein the organic matter content is ≥40%, the water content is ≤30%, and the pH value is 5.5-8.5.

[0058] 2) Weigh 0.3-0.5 parts of the composite bacterial agent prepared in Example 1, and mix it thoroughly with 1000 parts of the above-mentioned plant-derived organic fertilizer to obtain a biological organic fertilizer, wherein the organic matter is ≥40%, N ≥1.5%, P2O5 ≥2.5%, K2O ≥0.5%, and the effective viable bacteria count is ≥20 million / g.

[0059] Example 3 Application of composite microbial agent on corn and rice

[0060] The test site was the artificial climate chamber of Linyi Agricultural Research and Development Center of Sinochem Fertilizer Co., Ltd. in Linyi City, Shandong Province. The test crops were corn (Zhengdan 958) and rice (Shenyou 28). Hydroponics was used. The hydroponic bottles used in the experiment were black cylindrical bottles with a diameter of 10 cm and a height of 20 cm. 500 mL of 1 / 2 Hoagland culture medium was added to the hydroponic water.

[0061] The experiment set up 4 treatments, namely CK (blank treatment), T1 (Bacillus megaterium), T2 (Bacillus velez), T3 (composite microbial agent, the mass ratio of Bacillus megaterium and Bacillus velez is 1:1), T4 (composite microbial agent, the mass ratio of Bacillus megaterium and Bacillus velez is 0.5:1), T5 (composite microbial agent, the mass ratio of Bacillus megaterium and Bacillus velez is 1:0.5), and each treatment added bacterial powder according to the amount of live bacteria of 40 million / pot (the bacterial powder was dissolved in 500mL of 1 / 2 times of Hoagland culture solution). Twenty pots were planted in each treatment, and one corn seedling or five rice seedlings with consistent germination were immediately transplanted into each pot. Ten days after transplanting, data such as root system and aboveground biomass were collected, and the test results are shown in Table 1.

[0062] Table 1 Composite bacterial agent hydroponic test data

[0063]

[0064]

[0065] Experimental results: The composite bacterial agents T3, T4, and T5 formed by Bacillus megaterium and Bacillus Velez had significantly improved root-promoting effects and aboveground growth-promoting effects compared with CK, Bacillus megaterium alone, and Bacillus Velez alone (P>0.5). Refer to Table 1: Taking the effect of increasing root fresh weight of corn after 10 days of bacterial agent treatment as an example, when corn was applied with T3 (composite bacterial agent 1:1), the root fresh weight increased by 54.30% compared with CK at 10 days, while the root fresh weight of T1 (Bacillus megaterium) and T2 (Bacillus Velez) increased by 19.87% and 29.14% respectively compared with CK at 10 days, which was less than T3 (composite bacterial agent 1:1). Compared with CK, T3 (compound microbial agent 1:1) treatment showed the best root-promoting effect (corn root fresh weight increased by 54.3%, rice root fresh weight increased by 40.77%) at 10 days after transplanting, followed by aboveground growth-promoting effect (corn aboveground fresh weight increased by 12.43%, rice aboveground fresh weight increased by 17.22%); and T3 (compound microbial agent 1:1) treatment effect was better than T4 and T5. The above results show that T3 (compound microbial agent 1:1) treatment has a significant effect on the root growth of corn and rice in the transplanting seedling stage.

[0066] Example 4 Application of composite microbial agent on tomatoes and cucumbers

[0067] The test site was the artificial climate room of Linyi Agricultural Research and Development Center of Sinochem Fertilizer Co., Ltd. in Linyi City, Shandong Province. The test crops were tomatoes (pink tomatoes) and cucumbers (Shengdong No. 8). Potted planting was adopted. The test soil was taken from the brown soil in Linyi, Shandong. After removing impurities and screening, it was potted. Each pot contained 1 kg of soil. The specifications of the experimental pots were 15cm x 15cm square pots and the pot height was 15cm.

[0068] The experiment set up 6 treatments, namely CK (blank treatment), T1 (Bacillus megaterium), T2 (Bacillus velez), T3 (composite microbial agent, the mass ratio of Bacillus megaterium and Bacillus velez is 1:1), T4 (composite microbial agent, the mass ratio of Bacillus megaterium and Bacillus velez is 0.5:1), T5 (composite microbial agent, the mass ratio of Bacillus megaterium and Bacillus velez is 1:0.5), each treatment added bacterial powder according to the amount of live bacteria of 40 million / pot (after dilution with water, irrigate the roots). Twenty pots were planted in each treatment, and one tomato or cucumber seedling with the same growth was transplanted in each pot. The agent was irrigated once at the time of transplanting and 10 days after transplanting. 10 days after transplanting, the data of root system and aboveground biomass were collected. The test results are shown in Table 2.

[0069] Table 2 Composite microbial agent potted plant test data

[0070]

[0071] Experimental results: The composite bacterial agents T3, T4, and T5 formed by Bacillus megaterium and Bacillus Velez had significantly improved root-promoting effects and aboveground growth-promoting effects compared with CK, Bacillus megaterium alone, and Bacillus Velez alone (P>0.5). Refer to Table 2: Taking the effect of increasing root fresh weight of tomatoes after 10 days of bacterial agent treatment as an example, when tomatoes were applied with T3 (composite bacterial agent 1:1), the root fresh weight increased by 40.69% compared with CK at 10 days, while the root fresh weight of T1 (Bacillus megaterium) and T2 (Bacillus Velez) increased by 10.39% and 11.26% respectively compared with CK at 10 days, which was less than T3 (composite bacterial agent 1:1). Compared with CK, T3 (compound microbial agent 1:1) treatment showed the best root-promoting effect at 10 days after transplanting (tomato root fresh weight increased by 40.69%, cucumber root fresh weight increased by 40.6%), followed by aboveground growth-promoting effect (tomato aboveground fresh weight increased by 10.23%, cucumber aboveground fresh weight increased by 15.88%); and T3 (compound microbial agent 1:1) treatment effect was better than T4 and T5. The above results show that T3 (compound microbial agent 1:1) treatment has a significant effect on the root growth of tomatoes and cucumbers in the transplanting seedling stage.

[0072] Example 5 Application of composite microbial agent on potted peppers and dwarf tomatoes

[0073] The potted test was conducted at the greenhouse of Linyi Agricultural Research and Development Center of Sinochem Fertilizer Co., Ltd. in Linyi City, Shandong Province. The test crops were pepper (Keyu No. 8) and tomato (dwarf tomato). The test soil was taken from the slightly saline-alkali soil in Wudi, Binzhou (total salt content 1.8g / Kg). After removing impurities and screening, it was potted. Each pot contained 2.0Kg of soil. The experimental pots were 25cm in diameter and 20cm in height.

[0074] The experiment set up 4 treatments, namely CK (blank treatment), T1 (Bacillus megaterium), T2 (Bacillus velezii), T3 (composite microbial agent, the mass ratio of Bacillus megaterium and Bacillus velezii was 1:1), and each treatment added bacterial powder at a live bacteria amount of 40 million / pot (diluted with clean water and then irrigated the roots). Twenty pots were planted in each treatment, and one pepper seedling or tomato seedling with the same growth was immediately transplanted to each pot. The agent was applied once on the day of transplanting, 7 days after transplanting, and 14 days after transplanting. 15 days after transplanting, the data of root system and aboveground biomass were collected. The test results are shown in Table 3.

[0075] Table 3 Data of the microbial pot test

[0076]

[0077] The test results showed that the T3 (composite microbial agent 1; 1) treatment significantly increased the fresh weight of crop roots compared with CK in the seedling stage (the fresh weight of pepper roots increased by 25.64%, and the fresh weight of dwarf tomato roots increased by 24.55%), followed by the increase in the fresh weight of the aboveground parts of crops (the aboveground fresh weight of peppers increased by 12.18%, and the aboveground fresh weight of dwarf tomatoes increased by 10.04%. The above results show that the application of composite microbial agents can promote rapid rooting and seedling recovery of crops after transplanting, and the effect of applying composite microbial agents is better than that of applying single microbial agents.

[0078] Example 6 Application of biological organic fertilizer on potted tomatoes, peppers and cabbages

[0079] The potted test site is the greenhouse of Linyi Agricultural Research and Development Center of Sinochem Fertilizer Co., Ltd. in Linyi City, Shandong Province. The test crops are pepper (Keyu No. 8), tomato (dwarf tomato), and cabbage (Kuaicai No. 5). The test soil is taken from the slightly saline-alkali soil in Wudi, Binzhou (total salt content 1.8g / Kg). After impurities and screening, it is potted. Each pot contains 2000g of soil. The experimental pot specifications are round pots with a pot diameter of 20cm and a pot height of 15cm. The physical and chemical properties of the potted soil are: organic matter 1.35g / kg, pH value 8.35, alkaline nitrogen 123.1mg / kg, available phosphorus 18.2mg / kg, and available potassium 110mg / kg.

[0080] The experiment set up three treatments, namely CK (blank treatment), T1 (plant-derived organic fertilizer without compound bacteria, which is the same as the plant-derived organic fertilizer in T2), and T2 (compound fertilizer with compound bacteria, including compound bacteria and plant-derived organic fertilizer). Each treatment applied fertilizer at 100g / pot (converted to 500Kg / mu) and mixed it evenly with the potting soil in advance. Twenty pots were planted in each treatment, and one pepper seedling, tomato seedling or cabbage seedling with the same growth was transplanted or sown in each pot. According to the growth of crops, daily water and fertilizer management was carried out, crop yield data during the harvest period was collected, and changes in potting soil were analyzed. The test results are shown in Table 4.

[0081] Table 4 Data of bio-organic fertilizer in pot test

[0082]

[0083] From the test results, it can be seen that the application of biological organic fertilizer can effectively increase crop yields and improve the physical and chemical properties of saline-alkali soil. Specifically, compared with T1 (organic fertilizer without compound bacterial agent), the application of T2 (compound fertilizer with compound bacterial agent) can increase the yield of pepper, tomato and cabbage by 6.7%-9.2%, increase the soil organic matter content by 10.8%-28.3%, and reduce the soil EC value by 6.7%-10.5%. The above results show that the application of biological organic fertilizer has a significant effect on regulating saline-alkali soil and promoting crop yield.

[0084] Example 7 Application of biological organic fertilizer in potato fields

[0085] The experiments were carried out on continuous cropping soils in the main potato producing areas of Linyi City, Shandong Province, Feicheng City, Shandong Province, Shangdu County, Inner Mongolia, and Guyuan County, Hebei Province. Mechanized fertilization was used, with an organic fertilizer dosage of 500 kg / mu.

[0086] The experiment set up three treatments, namely CK (blank treatment), T1 (plant-derived organic fertilizer without compound bacteria, which is the same as the plant-derived organic fertilizer in T2), T2 (compound fertilizer with compound bacteria, including compound bacteria and plant-derived organic fertilizer), each treatment has 3 replicates, and each replicate is ≥50㎡. According to the growth of crops and local topdressing methods, daily water and fertilizer management was carried out, the incidence of common diseases in the middle and late stages of potatoes was investigated, the yield data during the harvest period was collected, and the soil changes after harvest were analyzed. The test results are shown in Table 5.

[0087] Table 5 Tomato pot test data

[0088]

[0089] From the test results, it can be seen that the application of biological organic fertilizer can effectively increase the yield of potatoes per mu, prevent and control a variety of soil-borne diseases, and increase the content of soil organic matter. Specifically, compared with T1 (plant-derived organic fertilizer without the addition of compound bacteria), the application of T2 (compound fertilizer with compound bacteria) can increase the yield of potatoes per mu by 8.8%-16.5%, prevent and control potato soil-borne diseases by 16.2%-42.2%, and increase the content of soil organic matter by 5.3%-8.1%. The above results show that the application of biological organic fertilizer has a significant effect on supplementing soil organic matter, promoting crop yield, and preventing and controlling soil-borne diseases. At the same time, the results show that the application of biological organic fertilizer is better than the application of ordinary organic fertilizer.

[0090] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0091] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0092] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A composite bacterial agent, characterized in that: include: The active ingredient comprises Bacillus megaterium and Bacillus velez. The deposit number of the Bacillus megaterium is CGMCC No. 21828, the deposit number of the Bacillus velez is CGMCC No. 28558, and the mass ratio of the Bacillus megaterium to the Bacillus velez is (0.5-1): (0.5-1).

2. The composite bacterial agent according to claim 1, characterized in that The mass ratio of the Bacillus megaterium to the Bacillus velezii is 1:

1.

3. The composite bacterial agent according to claim 1, characterized in that Based on the total mass of the composite bacterial agent, the amount of live bacteria of the active ingredient is 60 billion / g-100 billion / g.

4. The composite bacterial agent according to claim 1, characterized in that The ratio of the amount of live bacteria of the Bacillus megaterium to that of the Bacillus velez is (2-10):(6-15); preferably, the ratio of the amount of live bacteria of the Bacillus megaterium to that of the Bacillus velez is (3-5):(10-15).

5. The composite bacterial agent according to claim 1, characterized in that: The composite bacterial agent further includes auxiliary materials; Optionally, the auxiliary material includes at least one of calcium carbonate or diatomaceous earth; Preferably, the mass ratio of the excipient to the active ingredient is (0.5-2):(0.5-2); preferably 1:

1.

6. A compound fertilizer, characterized in that: The invention comprises the composite bacterial agent as described in any one of claims 1 to 5 and a plant-derived organic fertilizer.

7. The compound fertilizer according to claim 6, characterized in that The mass ratio of the composite bacterial agent to the plant-derived organic fertilizer is (3-5):10000; Preferably, based on the total mass of the compound fertilizer, the amount of live bacteria is not less than 20 million / g.

8. Use of the composite bacterial agent according to any one of claims 1 to 5 or the composite fertilizer according to any one of claims 6 to 7 in promoting the growth of crops or preventing and controlling crop diseases; Optionally, the crop growth includes at least one of root growth and aboveground growth of the crop; Optionally, the crops include at least one of corn, rice, tomato, pepper, cabbage and potato; Preferably, the crop growth includes at least one of corn, rice, tomato, pepper and cabbage; Preferably, the crop diseases include at least one of potato early blight, potato wilt, and potato scab.

9. A method for promoting crop growth or preventing and controlling crop diseases, characterized in that: Applying the composite bacterial agent according to any one of claims 1 to 5 or the composite fertilizer according to any one of claims 6 to 7 to the crops; Optionally, applying the compound bacterial agent or the compound fertilizer to at least one of the root and the leaf of the crop; Optionally, the application amount of the composite bacterial agent is 100-200 billion CFU / mu; Optionally, the application amount of the compound fertilizer is 30-60 kg / mu.

Images